AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25296

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25296.t1 MTR_8g062270 88.889 414 21 1 1 389 41 454 0.0 741
MS.gene25296.t1 MTR_8g468760 61.962 418 96 8 1 388 34 418 5.14e-163 464
MS.gene25296.t1 MTR_5g092960 57.957 421 136 7 3 388 47 461 5.62e-152 437
MS.gene25296.t1 MTR_8g468840 57.692 416 139 8 2 387 40 448 1.68e-148 428
MS.gene25296.t1 MTR_8g467800 51.914 418 159 8 2 388 46 452 6.16e-127 373
MS.gene25296.t1 MTR_2g083840 49.048 420 168 7 3 388 49 456 2.70e-118 351
MS.gene25296.t1 MTR_4g032730 47.344 433 172 10 3 388 43 466 4.94e-114 341
MS.gene25296.t1 MTR_5g092960 57.975 326 101 5 3 295 47 369 5.42e-112 333
MS.gene25296.t1 MTR_8g468810 45.794 428 176 12 1 388 51 462 1.43e-108 327
MS.gene25296.t1 MTR_8g058970 46.188 446 168 13 1 388 45 476 2.94e-104 316
MS.gene25296.t1 MTR_8g468810 47.222 324 131 8 1 292 51 366 1.07e-81 254
MS.gene25296.t1 MTR_8g014260 39.906 426 196 16 4 388 44 450 1.61e-75 241
MS.gene25296.t1 MTR_8g445780 43.213 361 153 9 4 335 41 378 3.79e-75 238
MS.gene25296.t1 MTR_8g058965 40.139 431 199 12 2 388 40 455 1.43e-72 234
MS.gene25296.t1 MTR_8g469020 36.490 433 213 10 2 388 34 450 7.16e-69 224
MS.gene25296.t1 MTR_8g468810 48.485 264 104 5 1 239 51 307 4.38e-68 218
MS.gene25296.t1 MTR_8g468810 48.485 264 104 5 1 239 51 307 4.41e-68 218
MS.gene25296.t1 MTR_8g468810 48.485 264 104 5 1 239 51 307 1.08e-67 218
MS.gene25296.t1 MTR_8g070950 38.242 421 199 14 1 388 41 433 2.29e-65 214
MS.gene25296.t1 MTR_8g062010 37.560 418 208 13 1 388 35 429 3.85e-64 211
MS.gene25296.t1 MTR_8g062310 37.530 421 180 17 4 388 54 427 1.70e-62 207
MS.gene25296.t1 MTR_8g467800 48.000 275 103 7 2 247 46 309 2.23e-62 204
MS.gene25296.t1 MTR_4g050250 40.053 377 176 9 1 335 47 415 5.26e-60 200
MS.gene25296.t1 MTR_8g062080 36.150 426 204 15 1 388 49 444 2.21e-58 196
MS.gene25296.t1 MTR_8g014210 47.083 240 112 7 154 388 1 230 2.43e-55 182
MS.gene25296.t1 MTR_8g062300 33.624 458 201 18 1 388 52 476 1.83e-52 182
MS.gene25296.t1 MTR_4g024710 40.149 269 145 7 126 388 88 346 2.62e-48 167
MS.gene25296.t1 MTR_8g077460 32.250 400 193 16 4 388 38 374 7.78e-45 159
MS.gene25296.t1 MTR_4g024745 38.790 281 157 7 95 370 10 280 2.71e-40 144
MS.gene25296.t1 MTR_8g014160 37.545 277 137 9 2 247 67 338 5.70e-38 142
MS.gene25296.t1 MTR_8g467790 31.956 363 169 12 1 333 174 488 4.43e-36 138
MS.gene25296.t1 MTR_2g086020 31.078 399 179 15 1 388 1 314 1.53e-32 125
MS.gene25296.t1 MTR_5g080710 29.976 417 226 18 2 381 59 446 3.67e-26 109
MS.gene25296.t1 MTR_5g084070 29.540 413 226 19 2 381 39 419 1.62e-25 107
MS.gene25296.t1 MTR_8g014290 44.628 121 60 5 4 118 38 157 5.72e-21 89.4
MS.gene25296.t1 MTR_5g021550 45.833 120 54 5 4 118 47 160 1.58e-20 88.2
MS.gene25296.t1 MTR_4g080787 27.924 419 241 22 2 376 45 446 8.90e-15 76.3
MS.gene25296.t1 MTR_4g024720 37.226 137 57 5 4 118 45 174 1.25e-13 69.7
MS.gene25296.t1 MTR_5g039950 27.807 374 215 15 2 357 49 385 2.61e-12 68.2
MS.gene25296.t1 MTR_4g056070 28.689 366 195 17 25 343 89 435 1.13e-11 66.2
MS.gene25296.t1 MTR_4g113810 32.215 149 74 4 78 199 18 166 2.70e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25296.t1 AT5G53840 25.178 421 252 17 2 386 44 437 4.78e-24 103
MS.gene25296.t1 AT1G16930 25.172 437 244 18 2 386 41 446 1.26e-17 84.7
MS.gene25296.t1 AT1G16930 25.172 437 244 18 2 386 41 446 1.26e-17 84.7
MS.gene25296.t1 AT1G16930 25.172 437 244 18 2 386 41 446 1.26e-17 84.7
MS.gene25296.t1 AT5G56700 27.195 353 188 17 46 356 22 347 3.12e-17 82.8
MS.gene25296.t1 AT5G53635 25.175 429 250 16 3 389 27 426 6.37e-17 82.4
MS.gene25296.t1 AT1G13780 25.566 442 247 18 2 386 36 452 1.08e-16 82.0
MS.gene25296.t1 AT5G38570 26.404 356 210 16 46 388 59 375 4.63e-16 79.3
MS.gene25296.t1 AT4G00160 30.155 388 200 22 2 351 42 396 1.01e-15 79.0
MS.gene25296.t1 AT1G78750 24.036 441 256 18 2 389 44 458 5.65e-15 76.6
MS.gene25296.t1 AT1G78750 24.036 441 256 18 2 389 44 458 5.65e-15 76.6
MS.gene25296.t1 AT5G56420 24.292 424 250 17 2 388 32 421 8.62e-15 75.9
MS.gene25296.t1 AT5G56420 24.292 424 250 17 2 388 32 421 8.62e-15 75.9
MS.gene25296.t1 AT5G56420 24.292 424 250 17 2 388 32 421 8.62e-15 75.9
MS.gene25296.t1 AT5G22660 25.445 393 215 18 56 389 73 446 9.25e-15 75.9
MS.gene25296.t1 AT5G27750 25.542 415 231 18 2 359 31 424 1.21e-14 75.5
MS.gene25296.t1 AT1G69630 22.851 442 253 16 2 389 44 451 1.87e-14 75.1
MS.gene25296.t1 AT3G52680 29.293 396 199 22 2 355 47 403 6.61e-14 73.6
MS.gene25296.t1 AT5G60610 26.209 393 215 18 2 356 28 383 7.97e-14 72.8
MS.gene25296.t1 AT5G60610 26.209 393 215 18 2 356 28 383 7.97e-14 72.8
MS.gene25296.t1 AT1G06630 25.387 323 199 9 2 292 38 350 1.12e-13 72.0
MS.gene25296.t1 AT1G06630 25.387 323 199 9 2 292 38 350 1.12e-13 72.0
MS.gene25296.t1 AT1G06630 25.000 336 205 10 2 300 38 363 1.23e-13 72.4
MS.gene25296.t1 AT1G06630 25.000 336 205 10 2 300 38 363 1.23e-13 72.4
MS.gene25296.t1 AT1G06630 25.000 336 205 10 2 300 38 363 1.23e-13 72.4
MS.gene25296.t1 AT1G06630 25.000 336 205 10 2 300 38 363 1.23e-13 72.4
MS.gene25296.t1 AT1G06630 25.000 336 205 10 2 300 47 372 1.50e-13 72.0
MS.gene25296.t1 AT5G56820 26.744 430 223 22 2 386 41 423 2.03e-13 71.6
MS.gene25296.t1 AT4G26340 23.239 426 253 16 2 388 28 418 2.11e-13 71.6
MS.gene25296.t1 AT3G50710 25.000 432 240 20 3 386 29 424 2.33e-13 71.6
MS.gene25296.t1 AT5G22730 23.476 443 248 17 2 386 54 463 2.33e-13 71.6
MS.gene25296.t1 AT5G22730 23.476 443 248 17 2 386 54 463 2.33e-13 71.6
MS.gene25296.t1 AT1G06630 25.000 336 205 10 2 300 38 363 2.72e-13 71.6
MS.gene25296.t1 AT1G13780 27.869 305 158 14 2 257 36 327 1.29e-12 68.6
MS.gene25296.t1 AT1G78760 22.274 431 257 19 2 386 42 440 2.41e-12 68.6
MS.gene25296.t1 AT5G22720 23.326 463 234 21 2 389 49 465 4.94e-12 67.8
MS.gene25296.t1 AT4G10400 25.635 394 214 21 2 359 28 378 5.38e-12 67.4
MS.gene25296.t1 AT4G10400 25.635 394 214 21 2 359 28 378 5.38e-12 67.4
MS.gene25296.t1 AT4G10400 25.635 394 214 21 2 359 28 378 5.38e-12 67.4
MS.gene25296.t1 AT4G10420 24.484 339 205 13 82 388 2 321 5.57e-12 66.6
MS.gene25296.t1 AT5G22610 23.460 422 256 20 2 387 44 434 7.86e-12 67.0
MS.gene25296.t1 AT5G22700 24.802 379 206 15 2 333 32 378 1.06e-11 66.6
MS.gene25296.t1 AT5G22700 24.802 379 206 15 2 333 32 378 1.06e-11 66.6
MS.gene25296.t1 AT5G22700 24.802 379 206 15 2 333 32 378 1.06e-11 66.6
MS.gene25296.t1 AT5G22700 24.802 379 206 15 2 333 32 378 1.06e-11 66.6
MS.gene25296.t1 AT5G22700 24.802 379 206 15 2 333 69 415 1.21e-11 66.6
MS.gene25296.t1 AT5G38386 23.433 367 243 13 1 359 27 363 1.28e-11 66.2
MS.gene25296.t1 AT5G38386 23.433 367 243 13 1 359 27 363 1.28e-11 66.2
MS.gene25296.t1 AT2G26860 26.185 401 215 19 2 356 28 393 1.46e-11 65.9
MS.gene25296.t1 AT2G26860 26.185 401 215 19 2 356 28 393 1.46e-11 65.9
MS.gene25296.t1 AT5G56370 26.047 430 238 18 2 388 28 420 2.11e-11 65.5
MS.gene25296.t1 AT5G56370 26.047 430 238 18 2 388 28 420 2.11e-11 65.5
MS.gene25296.t1 AT4G13985 21.860 430 275 14 1 386 44 456 4.50e-11 64.7
MS.gene25296.t1 AT5G56410 23.058 399 249 13 2 359 29 410 5.99e-11 64.3
MS.gene25296.t1 AT1G66310 24.648 426 255 17 2 389 45 442 7.16e-11 63.9

Find 93 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAATGAGCTGGAATTTAT+TGG 0.228561 8.2:-38307741 MS.gene25296:CDS
TTACTTTAGTCCTGAATCTT+TGG 0.234049 8.2:-38308489 MS.gene25296:CDS
AAACTCCGGAAAATGTAAAA+TGG 0.241590 8.2:+38308077 None:intergenic
CTGAATCTTTGGACATAATT+CGG 0.268479 8.2:-38308478 MS.gene25296:CDS
ATTCGTTGCCTTCATATAGA+TGG 0.301405 8.2:-38307836 MS.gene25296:CDS
AAATGGGATATGAGGCATTT+AGG 0.311419 8.2:+38307792 None:intergenic
CGAATTATGTCCAAAGATTC+AGG 0.318966 8.2:+38308479 None:intergenic
CAATGATTGTTGATGAATAT+TGG 0.328994 8.2:-38307686 MS.gene25296:CDS
AATCTCCTTCGAGCTCTTTC+CGG 0.331425 8.2:-38308248 MS.gene25296:CDS
TTCCGTGATGCCGCGGCTGT+TGG 0.352559 8.2:-38308347 MS.gene25296:CDS
AGAGGGTGAGATTAAATTCC+TGG 0.363398 8.2:+38309020 None:intergenic
AAAATGAGCTGGAATTTATT+GGG 0.368042 8.2:-38307740 MS.gene25296:CDS
CTCAAGTACCTTAGCCTTGA+AGG 0.369562 8.2:-38308377 MS.gene25296:CDS
TCCGTGATGCCGCGGCTGTT+GGG 0.374852 8.2:-38308346 MS.gene25296:CDS
TCCGTCGCTCACGCGTCGTT+CGG 0.384887 8.2:-38309135 MS.gene25296:CDS
AAAAGTGAATAAAGGTCAAA+TGG 0.385833 8.2:+38307775 None:intergenic
CTCGCAAATGGCGCAACCTC+TGG 0.386923 8.2:-38309279 MS.gene25296:CDS
TGAATCTTTGGACATAATTC+GGG 0.390671 8.2:-38308477 MS.gene25296:CDS
CTGTCCTGTTCTCGAGAATC+TGG 0.392606 8.2:-38308516 MS.gene25296:CDS
TAGTTCTAGACGTTTAATTC+CGG 0.398389 8.2:+38308229 None:intergenic
ATCACTGTTGATAATAATGT+TGG 0.404587 8.2:-38308428 MS.gene25296:CDS
AACTCCGGAAAATGTAAAAT+GGG 0.406590 8.2:+38308078 None:intergenic
CGCCATTGGGCCCCACCTCC+AGG 0.411986 8.2:-38309038 MS.gene25296:CDS
TCACTGTTGATAATAATGTT+GGG 0.420825 8.2:-38308427 MS.gene25296:CDS
AAGAGAAGAGTGTGAGAGGT+AGG 0.423108 8.2:+38308975 None:intergenic
AAGCAAAGAATGGATCGTTT+AGG 0.433146 8.2:+38309086 None:intergenic
TTTAACCTCTTCAAGGAAGA+TGG 0.437384 8.2:+38308452 None:intergenic
TGTTCGTTGAATTCGTCATC+GGG 0.437656 8.2:+38309199 None:intergenic
GAAAACTTCAGTCACCTTTG+TGG 0.437723 8.2:+38308196 None:intergenic
AATCTCACCCTCTTTACTGC+TGG 0.442889 8.2:-38309010 MS.gene25296:CDS
CACGGAATGTGATCCCTTCA+AGG 0.450691 8.2:+38308363 None:intergenic
AGTTCCAGATTCTCGAGAAC+AGG 0.455066 8.2:+38308512 None:intergenic
TAAAGGTCAAATGGGATATG+AGG 0.466574 8.2:+38307784 None:intergenic
TTGATAATAATGTTGGGGCT+TGG 0.473819 8.2:-38308421 MS.gene25296:CDS
TTGTTCGTTGAATTCGTCAT+CGG 0.482869 8.2:+38309198 None:intergenic
TGAGCCTCGTCTCTCGCAAA+TGG 0.483372 8.2:-38309291 MS.gene25296:CDS
CGTGGCTCAGTATCGCCATT+GGG 0.488486 8.2:-38309051 MS.gene25296:CDS
GCTATGAGAGTTTCTAGCAT+TGG 0.489509 8.2:+38307979 None:intergenic
GAGCAAGAGAAGAGTGTGAG+AGG 0.493002 8.2:+38308971 None:intergenic
TTCGTTGCCTTCATATAGAT+GGG 0.495093 8.2:-38307835 MS.gene25296:CDS
ACATCCGTAAAAGTGAATAA+AGG 0.501125 8.2:+38307767 None:intergenic
TTCCTGGAGGTGGGGCCCAA+TGG 0.507405 8.2:+38309036 None:intergenic
GAGATTAAATTCCTGGAGGT+GGG 0.510070 8.2:+38309027 None:intergenic
AAAGTGAATAAAGGTCAAAT+GGG 0.512234 8.2:+38307776 None:intergenic
TGTGAGAGGTAGGTTGTTGA+AGG 0.512486 8.2:+38308985 None:intergenic
TGAGATTAAATTCCTGGAGG+TGG 0.517495 8.2:+38309026 None:intergenic
GAGGACAGTGTTGACGAAAA+CGG 0.521173 8.2:+38309162 None:intergenic
TGGCACATGGCAGATCCTCT+TGG 0.523168 8.2:+38307621 None:intergenic
CACTGTTGATAATAATGTTG+GGG 0.535843 8.2:-38308426 MS.gene25296:CDS
TCGACTGAGTAAGCAAAGAA+TGG 0.539936 8.2:+38309076 None:intergenic
CAGATTCTCGAGAACAGGAC+AGG 0.544753 8.2:+38308517 None:intergenic
GGCGAGACCAACCAGACCGC+TGG 0.544821 8.2:-38307665 MS.gene25296:CDS
ACGTGGCTCAGTATCGCCAT+TGG 0.547510 8.2:-38309052 MS.gene25296:CDS
TCCGAACGACGCGTGAGCGA+CGG 0.553880 8.2:+38309134 None:intergenic
TTTGCCTCCCATCTATATGA+AGG 0.559497 8.2:+38307828 None:intergenic
CGCGTGAGCGACGGAGAGAG+AGG 0.560043 8.2:+38309143 None:intergenic
ATGTGATCCCTTCAAGGCTA+AGG 0.560362 8.2:+38308369 None:intergenic
TCAGTCAGAGAAATTTAACT+TGG 0.568365 8.2:+38307601 None:intergenic
TGAAGGCACCAGCAGTAAAG+AGG 0.569444 8.2:+38309002 None:intergenic
TGAAGCAAATAGTGAAACTC+CGG 0.573562 8.2:+38308063 None:intergenic
CGGATGTCCAGAAAATGAGC+TGG 0.574811 8.2:-38307751 MS.gene25296:CDS
AGAATGGATCGTTTAGGATG+TGG 0.575368 8.2:+38309092 None:intergenic
TCCCAACAGCCGCGGCATCA+CGG 0.577725 8.2:+38308345 None:intergenic
GGGTGCCATCTTCCTTGAAG+AGG 0.580167 8.2:-38308457 MS.gene25296:CDS
GAACAGGACAGGCAGAGAGA+AGG 0.583613 8.2:+38308528 None:intergenic
TCAAGTACCTTAGCCTTGAA+GGG 0.585522 8.2:-38308376 MS.gene25296:CDS
GGATGTGGTCGGAGGAGAGA+CGG 0.588995 8.2:+38309107 None:intergenic
AGATTAAATTCCTGGAGGTG+GGG 0.594581 8.2:+38309028 None:intergenic
TTCGTTGAATTCGTCATCGG+GGG 0.595692 8.2:+38309201 None:intergenic
AGACTTCGAGATTCTTCCAG+AGG 0.596831 8.2:+38309263 None:intergenic
GAAGGCACCAGCAGTAAAGA+GGG 0.604956 8.2:+38309003 None:intergenic
GATCACATTCCGTGATGCCG+CGG 0.609193 8.2:-38308354 MS.gene25296:CDS
TGGCTTGAAATAGATGCACC+TGG 0.612723 8.2:-38308401 MS.gene25296:CDS
TTGCTTACTCAGTCGACACG+TGG 0.615356 8.2:-38309069 MS.gene25296:CDS
GCTCGAAGGAGATTGAGTAA+AGG 0.617059 8.2:+38308257 None:intergenic
GGCAGAGAGAAGGATATGCA+TGG 0.624602 8.2:+38308538 None:intergenic
AGGCTAAGGTACTTGAGACC+AGG 0.624770 8.2:+38308383 None:intergenic
ATTTAGTTTCGACATCGACA+TGG 0.626202 8.2:-38307956 MS.gene25296:intron
GTGCAAGTTCCCAACAGCCG+CGG 0.628805 8.2:+38308337 None:intergenic
ACTCCGGAAAATGTAAAATG+GGG 0.632256 8.2:+38308079 None:intergenic
AAAATTTCAGATCTACCAAG+AGG 0.633493 8.2:-38307636 MS.gene25296:CDS
GAGAAATTTAACTTGGCACA+TGG 0.635924 8.2:+38307608 None:intergenic
TGCGCCATTTGCGAGAGACG+AGG 0.637473 8.2:+38309287 None:intergenic
TGTATTCATTGCATTTGTCG+CGG 0.641710 8.2:+38309233 None:intergenic
ATCGTTTAGGATGTGGTCGG+AGG 0.648456 8.2:+38309099 None:intergenic
GTTCGTTGAATTCGTCATCG+GGG 0.649274 8.2:+38309200 None:intergenic
CTCCGGAAAATGTAAAATGG+GGG 0.656185 8.2:+38308080 None:intergenic
TGGATCGTTTAGGATGTGGT+CGG 0.656581 8.2:+38309096 None:intergenic
ACTACATAATTCAACCACAA+AGG 0.657271 8.2:-38308210 MS.gene25296:intron
TAATTCCGGAAAGAGCTCGA+AGG 0.661187 8.2:+38308243 None:intergenic
GTTGCCTTCATATAGATGGG+AGG 0.669069 8.2:-38307832 MS.gene25296:CDS
TGTTGGGAACTTGCACAGCG+TGG 0.697966 8.2:-38308330 MS.gene25296:CDS
GGGTGAGATTAAATTCCTGG+AGG 0.740357 8.2:+38309023 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACAAAAATAAAGAAAAG+AGG + chr8.2:38307993-38308012 None:intergenic 15.0%
!! GCTATATATAAGAGAAAATT+GGG + chr8.2:38308110-38308129 None:intergenic 20.0%
!! TGCTATATATAAGAGAAAAT+TGG + chr8.2:38308111-38308130 None:intergenic 20.0%
! AAAAGTGAATAAAGGTCAAA+TGG + chr8.2:38309125-38309144 None:intergenic 25.0%
! AAAATGAGCTGGAATTTATT+GGG - chr8.2:38309157-38309176 MS.gene25296:CDS 25.0%
! AAAGTGAATAAAGGTCAAAT+GGG + chr8.2:38309124-38309143 None:intergenic 25.0%
! TAAAGAAAAGAGGATGAAAT+AGG + chr8.2:38307983-38308002 None:intergenic 25.0%
!! CAATGATTGTTGATGAATAT+TGG - chr8.2:38309211-38309230 MS.gene25296:CDS 25.0%
!! TTTTCTCTTATATATAGCAC+CGG - chr8.2:38308112-38308131 MS.gene25296:intron 25.0%
!!! AACAAAAAAGTTGAGTTGAA+AGG + chr8.2:38308879-38308898 None:intergenic 25.0%
!!! ACAAAAAAGTTGAGTTGAAA+GGG + chr8.2:38308878-38308897 None:intergenic 25.0%
!!! ATCACTGTTGATAATAATGT+TGG - chr8.2:38308469-38308488 MS.gene25296:CDS 25.0%
!!! TCACTGTTGATAATAATGTT+GGG - chr8.2:38308470-38308489 MS.gene25296:CDS 25.0%
!!! TTGACCTTTATTCACTTTTA+CGG - chr8.2:38309126-38309145 MS.gene25296:CDS 25.0%
AAAATTTCAGATCTACCAAG+AGG - chr8.2:38309261-38309280 MS.gene25296:CDS 30.0%
AAACTCCGGAAAATGTAAAA+TGG + chr8.2:38308823-38308842 None:intergenic 30.0%
AACTCCGGAAAATGTAAAAT+GGG + chr8.2:38308822-38308841 None:intergenic 30.0%
ACATCCGTAAAAGTGAATAA+AGG + chr8.2:38309133-38309152 None:intergenic 30.0%
ACTACATAATTCAACCACAA+AGG - chr8.2:38308687-38308706 MS.gene25296:intron 30.0%
ATCAGGTTCAAAATTTGGAT+CGG - chr8.2:38308064-38308083 MS.gene25296:CDS 30.0%
CTGAATCTTTGGACATAATT+CGG - chr8.2:38308419-38308438 MS.gene25296:CDS 30.0%
GAAAATGAGCTGGAATTTAT+TGG - chr8.2:38309156-38309175 MS.gene25296:CDS 30.0%
GATGTATCAGGTTCAAAATT+TGG - chr8.2:38308059-38308078 MS.gene25296:CDS 30.0%
TAAAGTTAAACTACTTCCTC+CGG + chr8.2:38308134-38308153 None:intergenic 30.0%
TGAATCTTTGGACATAATTC+GGG - chr8.2:38308420-38308439 MS.gene25296:CDS 30.0%
TTACTTTAGTCCTGAATCTT+TGG - chr8.2:38308408-38308427 MS.gene25296:CDS 30.0%
! AGTGATTTTTAACCTCTTCA+AGG + chr8.2:38308455-38308474 None:intergenic 30.0%
! ATAAATTCCAGCTCATTTTC+TGG + chr8.2:38309156-38309175 None:intergenic 30.0%
! ATTGGGTATATTTTGCAACA+TGG - chr8.2:38309174-38309193 MS.gene25296:CDS 30.0%
! CATTCCATTGTTATTGTCTA+GGG - chr8.2:38308016-38308035 MS.gene25296:CDS 30.0%
!! AAAAAAGTTGAGTTGAAAGG+GGG + chr8.2:38308876-38308895 None:intergenic 30.0%
!! AATTGGAAAGTTGATGTATC+AGG - chr8.2:38308047-38308066 MS.gene25296:CDS 30.0%
!! CAAAAAAGTTGAGTTGAAAG+GGG + chr8.2:38308877-38308896 None:intergenic 30.0%
!! CACTGTTGATAATAATGTTG+GGG - chr8.2:38308471-38308490 MS.gene25296:CDS 30.0%
!! TAGTTCTAGACGTTTAATTC+CGG + chr8.2:38308671-38308690 None:intergenic 30.0%
!!! CTGAAATTTTTTTCATCCAG+CGG + chr8.2:38309251-38309270 None:intergenic 30.0%
!!! GTGTTCGTGTTTTTTTTGTT+TGG - chr8.2:38308991-38309010 MS.gene25296:CDS 30.0%
AAGCAAAGAATGGATCGTTT+AGG + chr8.2:38307814-38307833 None:intergenic 35.0%
ACTCCGGAAAATGTAAAATG+GGG + chr8.2:38308821-38308840 None:intergenic 35.0%
ATGTAATGTAACAGTCTCAG+TGG - chr8.2:38308241-38308260 MS.gene25296:CDS 35.0%
ATTCGTTGCCTTCATATAGA+TGG - chr8.2:38309061-38309080 MS.gene25296:CDS 35.0%
CGAATTATGTCCAAAGATTC+AGG + chr8.2:38308421-38308440 None:intergenic 35.0%
CTAACCCTAGACAATAACAA+TGG + chr8.2:38308023-38308042 None:intergenic 35.0%
TAAAGGTCAAATGGGATATG+AGG + chr8.2:38309116-38309135 None:intergenic 35.0%
TGAAGCAAATAGTGAAACTC+CGG + chr8.2:38308837-38308856 None:intergenic 35.0%
TGTATTCATTGCATTTGTCG+CGG + chr8.2:38307667-38307686 None:intergenic 35.0%
TTCGTTGCCTTCATATAGAT+GGG - chr8.2:38309062-38309081 MS.gene25296:CDS 35.0%
TTGTTCGTTGAATTCGTCAT+CGG + chr8.2:38307702-38307721 None:intergenic 35.0%
TTTAACCTCTTCAAGGAAGA+TGG + chr8.2:38308448-38308467 None:intergenic 35.0%
! AAATGGGATATGAGGCATTT+AGG + chr8.2:38309108-38309127 None:intergenic 35.0%
! ATTTAGTTTCGACATCGACA+TGG - chr8.2:38308941-38308960 MS.gene25296:intron 35.0%
! GCATTCCATTGTTATTGTCT+AGG - chr8.2:38308015-38308034 MS.gene25296:CDS 35.0%
! TGTCTAGGGTTAGAGAAAAT+TGG - chr8.2:38308030-38308049 MS.gene25296:CDS 35.0%
! TTGATAATAATGTTGGGGCT+TGG - chr8.2:38308476-38308495 MS.gene25296:CDS 35.0%
!! ATCAGATTCACTTTTAGGAG+TGG + chr8.2:38308617-38308636 None:intergenic 35.0%
!! GAGAAATTTAACTTGGCACA+TGG + chr8.2:38309292-38309311 None:intergenic 35.0%
!! TCAGATTCACTTTTAGGAGT+GGG + chr8.2:38308616-38308635 None:intergenic 35.0%
AAATAGCCACTGCTTGTACA+CGG + chr8.2:38308793-38308812 None:intergenic 40.0%
AATCAAATGATCGATCCACC+TGG + chr8.2:38309044-38309063 None:intergenic 40.0%
ACGGTAAGCAAATTTCCGAA+GGG + chr8.2:38308291-38308310 None:intergenic 40.0%
AGAGGGTGAGATTAAATTCC+TGG + chr8.2:38307880-38307899 None:intergenic 40.0%
AGATTAAATTCCTGGAGGTG+GGG + chr8.2:38307872-38307891 None:intergenic 40.0%
CAACAAAGCTCGAGTTAAAC+TGG + chr8.2:38308972-38308991 None:intergenic 40.0%
CTCCGGAAAATGTAAAATGG+GGG + chr8.2:38308820-38308839 None:intergenic 40.0%
GAAAACTTCAGTCACCTTTG+TGG + chr8.2:38308704-38308723 None:intergenic 40.0%
GAGATTAAATTCCTGGAGGT+GGG + chr8.2:38307873-38307892 None:intergenic 40.0%
TCGACTGAGTAAGCAAAGAA+TGG + chr8.2:38307824-38307843 None:intergenic 40.0%
TGAGATTAAATTCCTGGAGG+TGG + chr8.2:38307874-38307893 None:intergenic 40.0%
TGTTCGTTGAATTCGTCATC+GGG + chr8.2:38307701-38307720 None:intergenic 40.0%
TTTGCCTCCCATCTATATGA+AGG + chr8.2:38309072-38309091 None:intergenic 40.0%
! AATTTGCTTACCGTCGTTGA+AGG - chr8.2:38308297-38308316 MS.gene25296:CDS 40.0%
! AGAATGGATCGTTTAGGATG+TGG + chr8.2:38307808-38307827 None:intergenic 40.0%
! TCTCTTATATATAGCACCGG+AGG - chr8.2:38308115-38308134 MS.gene25296:intron 40.0%
! TGCCCCCATTTTACATTTTC+CGG - chr8.2:38308815-38308834 MS.gene25296:intron 40.0%
!! GCTATGAGAGTTTCTAGCAT+TGG + chr8.2:38308921-38308940 None:intergenic 40.0%
!! GGCTCATCAGATTCACTTTT+AGG + chr8.2:38308622-38308641 None:intergenic 40.0%
!! TCAAGTACCTTAGCCTTGAA+GGG - chr8.2:38308521-38308540 MS.gene25296:CDS 40.0%
!!! ATTTTTTTCATCCAGCGGTC+TGG + chr8.2:38309246-38309265 None:intergenic 40.0%
AATCTCACCCTCTTTACTGC+TGG - chr8.2:38307887-38307906 MS.gene25296:intron 45.0%
AATCTCCTTCGAGCTCTTTC+CGG - chr8.2:38308649-38308668 MS.gene25296:intron 45.0%
AGACTTCGAGATTCTTCCAG+AGG + chr8.2:38307637-38307656 None:intergenic 45.0%
AGTTCCAGATTCTCGAGAAC+AGG + chr8.2:38308388-38308407 None:intergenic 45.0%
ATGTGATCCCTTCAAGGCTA+AGG + chr8.2:38308531-38308550 None:intergenic 45.0%
ATTTCCGAAGGGTCTTGCAA+TGG + chr8.2:38308280-38308299 None:intergenic 45.0%
GACGGTAAGCAAATTTCCGA+AGG + chr8.2:38308292-38308311 None:intergenic 45.0%
GCTCGAAGGAGATTGAGTAA+AGG + chr8.2:38308643-38308662 None:intergenic 45.0%
GGGTGAGATTAAATTCCTGG+AGG + chr8.2:38307877-38307896 None:intergenic 45.0%
GTTCGTTGAATTCGTCATCG+GGG + chr8.2:38307700-38307719 None:intergenic 45.0%
GTTGCCTTCATATAGATGGG+AGG - chr8.2:38309065-38309084 MS.gene25296:CDS 45.0%
TAATTCCGGAAAGAGCTCGA+AGG + chr8.2:38308657-38308676 None:intergenic 45.0%
TGGCTTGAAATAGATGCACC+TGG - chr8.2:38308496-38308515 MS.gene25296:CDS 45.0%
TGTGAGAGGTAGGTTGTTGA+AGG + chr8.2:38307915-38307934 None:intergenic 45.0%
TTCGTTGAATTCGTCATCGG+GGG + chr8.2:38307699-38307718 None:intergenic 45.0%
! AAGAGAAGAGTGTGAGAGGT+AGG + chr8.2:38307925-38307944 None:intergenic 45.0%
! TGGATCGTTTAGGATGTGGT+CGG + chr8.2:38307804-38307823 None:intergenic 45.0%
!! CTCAAGTACCTTAGCCTTGA+AGG - chr8.2:38308520-38308539 MS.gene25296:CDS 45.0%
!! GAGGACAGTGTTGACGAAAA+CGG + chr8.2:38307738-38307757 None:intergenic 45.0%
AGTTCCAGCACCTTCAACGA+CGG + chr8.2:38308310-38308329 None:intergenic 50.0%
ATCGATCCACCTGGCAACAA+GGG + chr8.2:38309035-38309054 None:intergenic 50.0%
CACGGAATGTGATCCCTTCA+AGG + chr8.2:38308537-38308556 None:intergenic 50.0%
CAGATTCTCGAGAACAGGAC+AGG + chr8.2:38308383-38308402 None:intergenic 50.0%
CGGATGTCCAGAAAATGAGC+TGG - chr8.2:38309146-38309165 MS.gene25296:CDS 50.0%
CTGTCCTGTTCTCGAGAATC+TGG - chr8.2:38308381-38308400 MS.gene25296:CDS 50.0%
GCTCTAACCTTGTCTCTCTC+AGG - chr8.2:38307945-38307964 MS.gene25296:intron 50.0%
GGCAGAGAGAAGGATATGCA+TGG + chr8.2:38308362-38308381 None:intergenic 50.0%
GTTGCCATTGCAAGACCCTT+CGG - chr8.2:38308273-38308292 MS.gene25296:CDS 50.0%
TTGCATGCCTGAGAGAGACA+AGG + chr8.2:38307955-38307974 None:intergenic 50.0%
TTGCTTACTCAGTCGACACG+TGG - chr8.2:38307828-38307847 MS.gene25296:CDS 50.0%
! AGGCTAAGGTACTTGAGACC+AGG + chr8.2:38308517-38308536 None:intergenic 50.0%
! ATCGTTTAGGATGTGGTCGG+AGG + chr8.2:38307801-38307820 None:intergenic 50.0%
! CTTTTGCCGTGTACAAGCAG+TGG - chr8.2:38308784-38308803 MS.gene25296:intron 50.0%
! GAGCAAGAGAAGAGTGTGAG+AGG + chr8.2:38307929-38307948 None:intergenic 50.0%
! TTTTCATCCAGCGGTCTGGT+TGG + chr8.2:38309242-38309261 None:intergenic 50.0%
!! GAAGGCACCAGCAGTAAAGA+GGG + chr8.2:38307897-38307916 None:intergenic 50.0%
!! TGAAGGCACCAGCAGTAAAG+AGG + chr8.2:38307898-38307917 None:intergenic 50.0%
ACGTGGCTCAGTATCGCCAT+TGG - chr8.2:38307845-38307864 MS.gene25296:CDS 55.0%
ATCAGTTGCCCCTTGTTGCC+AGG - chr8.2:38309023-38309042 MS.gene25296:CDS 55.0%
CGTGGCTCAGTATCGCCATT+GGG - chr8.2:38307846-38307865 MS.gene25296:CDS 55.0%
GAACAGGACAGGCAGAGAGA+AGG + chr8.2:38308372-38308391 None:intergenic 55.0%
GATCACATTCCGTGATGCCG+CGG - chr8.2:38308543-38308562 MS.gene25296:CDS 55.0%
GATCGATCCACCTGGCAACA+AGG + chr8.2:38309036-38309055 None:intergenic 55.0%
TCGATCCACCTGGCAACAAG+GGG + chr8.2:38309034-38309053 None:intergenic 55.0%
TGAATTCGTCATCGGGGGCT+TGG + chr8.2:38307694-38307713 None:intergenic 55.0%
TGAGCCTCGTCTCTCGCAAA+TGG - chr8.2:38307606-38307625 MS.gene25296:CDS 55.0%
TGGCACATGGCAGATCCTCT+TGG + chr8.2:38309279-38309298 None:intergenic 55.0%
TGTTGGGAACTTGCACAGCG+TGG - chr8.2:38308567-38308586 MS.gene25296:CDS 55.0%
! CTTACCGTCGTTGAAGGTGC+TGG - chr8.2:38308303-38308322 MS.gene25296:CDS 55.0%
! GGGTGCCATCTTCCTTGAAG+AGG - chr8.2:38308440-38308459 MS.gene25296:CDS 55.0%
AGTTGCCCCTTGTTGCCAGG+TGG - chr8.2:38309026-38309045 MS.gene25296:CDS 60.0%
CTCGCAAATGGCGCAACCTC+TGG - chr8.2:38307618-38307637 MS.gene25296:CDS 60.0%
GGATGTGGTCGGAGGAGAGA+CGG + chr8.2:38307793-38307812 None:intergenic 60.0%
GTGCAAGTTCCCAACAGCCG+CGG + chr8.2:38308563-38308582 None:intergenic 60.0%
TGCGCCATTTGCGAGAGACG+AGG + chr8.2:38307613-38307632 None:intergenic 60.0%
TCCCAACAGCCGCGGCATCA+CGG + chr8.2:38308555-38308574 None:intergenic 65.0%
TCCGAACGACGCGTGAGCGA+CGG + chr8.2:38307766-38307785 None:intergenic 65.0%
TCCGTCGCTCACGCGTCGTT+CGG - chr8.2:38307762-38307781 MS.gene25296:CDS 65.0%
TCCGTGATGCCGCGGCTGTT+GGG - chr8.2:38308551-38308570 MS.gene25296:CDS 65.0%
TTCCGTGATGCCGCGGCTGT+TGG - chr8.2:38308550-38308569 MS.gene25296:CDS 65.0%
TTCCTGGAGGTGGGGCCCAA+TGG + chr8.2:38307864-38307883 None:intergenic 65.0%
CGCGTGAGCGACGGAGAGAG+AGG + chr8.2:38307757-38307776 None:intergenic 70.0%
GGCGAGACCAACCAGACCGC+TGG - chr8.2:38309232-38309251 MS.gene25296:CDS 70.0%
CGCCATTGGGCCCCACCTCC+AGG - chr8.2:38307859-38307878 MS.gene25296:intron 75.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 38307605 38309314 38307605 ID=MS.gene25296
chr8.2 mRNA 38307605 38309314 38307605 ID=MS.gene25296.t1;Parent=MS.gene25296
chr8.2 exon 38308964 38309314 38308964 ID=MS.gene25296.t1.exon1;Parent=MS.gene25296.t1
chr8.2 CDS 38308964 38309314 38308964 ID=cds.MS.gene25296.t1;Parent=MS.gene25296.t1
chr8.2 exon 38308211 38308600 38308211 ID=MS.gene25296.t1.exon2;Parent=MS.gene25296.t1
chr8.2 CDS 38308211 38308600 38308211 ID=cds.MS.gene25296.t1;Parent=MS.gene25296.t1
chr8.2 exon 38307957 38308115 38307957 ID=MS.gene25296.t1.exon3;Parent=MS.gene25296.t1
chr8.2 CDS 38307957 38308115 38307957 ID=cds.MS.gene25296.t1;Parent=MS.gene25296.t1
chr8.2 exon 38307605 38307874 38307605 ID=MS.gene25296.t1.exon4;Parent=MS.gene25296.t1
chr8.2 CDS 38307605 38307874 38307605 ID=cds.MS.gene25296.t1;Parent=MS.gene25296.t1
Gene Sequence

>MS.gene25296

ATGAGCCTCGTCTCTCGCAAATGGCGCAACCTCTGGAAGAATCTCGAAGTCTTCGATTTCCGCGACAAATGCAATGAATACAGCTACCAAGCCCCCGATGACGAATTCAACGAACAATTCATGCTGTTCACCGTTTTCGTCAACACTGTCCTCTCTCTCCGTCGCTCACGCGTCGTTCGGAAGTTCCGTCTCTCCTCCGACCACATCCTAAACGATCCATTCTTTGCTTACTCAGTCGACACGTGGCTCAGTATCGCCATTGGGCCCCACCTCCAGGAATTTAATCTCACCCTCTTTACTGCTGGTGCCTTCAACAACCTACCTCTCACACTCTTCTCTTGCTCTAACCTTGTCTCTCTCAGGCATGCAACAATTCCTATTTCATCCTCTTTTCTTTATTTTTGTTTTCTGCATTCCATTGTTATTGTCTAGGGTTAGAGAAAATTGGAAAGTTGATGTATCAGGTTCAAAATTTGGATCGGATACATCAACTTTTTGTTAACCCAATTTTCTCTTATATATAGCACCGGAGGAAGTAGTTTAACTTTAATTTTTATGCTTGTTCAATTTAGCTGTTTTGATTTAATTTAAATGAGATATGATACATGATGATAACTTAACATAACACATTCATGAATGTAATGTAACAGTCTCAGTGGATATATATTGTTGCCATTGCAAGACCCTTCGGAAATTTGCTTACCGTCGTTGAAGGTGCTGGAACTACTACAAATGCATCACTTGAATTTGAGTTCCATGCATATCCTTCTCTCTGCCTGTCCTGTTCTCGAGAATCTGGAACTTTACTTTAGTCCTGAATCTTTGGACATAATTCGGGTGCCATCTTCCTTGAAGAGGTTAAAAATCACTGTTGATAATAATGTTGGGGCTTGGCTTGAAATAGATGCACCTGGTCTCAAGTACCTTAGCCTTGAAGGGATCACATTCCGTGATGCCGCGGCTGTTGGGAACTTGCACAGCGTGGAAGAAGCATATCTTGATGTATTTCCCACTCCTAAAAGTGAATCTGATGAGCCTTTACTCAATCTCCTTCGAGCTCTTTCCGGAATTAAACGTCTAGAACTACATAATTCAACCACAAAGGTGACTGAAGTTTTCATTATTTGTAGTATATGTTGTTTTGTAGTAATAATTATTAAATAATCATTTAATCACTTGCTTTTGCCGTGTACAAGCAGTGGCTATTTGCTGCCCCCATTTTACATTTTCCGGAGTTTCACTATTTGCTTCATCTAAAGCTTAAGTATCCCCCTTTCAACTCAACTTTTTTGTTCGACGTGCTTCAAAAATGTCCAATGCTAGAAACTCTCATAGCATTTAGTTTCGACATCGACATGGTTTGTCCAGTTTAACTCGAGCTTTGTTGTGTTCGTGTTTTTTTTGTTTGGATCTTATATATCAGTTGCCCCTTGTTGCCAGGTGGATCGATCATTTGATTCGTTGCCTTCATATAGATGGGAGGCAAAGTCTAAAAGTGTTCCTAAATGCCTCATATCCCATTTGACCTTTATTCACTTTTACGGATGTCCAGAAAATGAGCTGGAATTTATTGGGTATATTTTGCAACATGGACTTGTTTTGAAGACAATGATTGTTGATGAATATTGGCGAGACCAACCAGACCGCTGGATGAAAAAAATTTCAGATCTACCAAGAGGATCTGCCATGTGCCAAGTTAAATTTCTCTGA
Protein sequence

>MS.gene25296.t1

MSLVSRKWRNLWKNLEVFDFRDKCNEYSYQAPDDEFNEQFMLFTVFVNTVLSLRRSRVVRKFRLSSDHILNDPFFAYSVDTWLSIAIGPHLQEFNLTLFTAGAFNNLPLTLFSCSNLVLELLQMHHLNLSSMHILLSACPVLENLELYFSPESLDIIRVPSSLKRLKITVDNNVGAWLEIDAPGLKYLSLEGITFRDAAAVGNLHSVEEAYLDVFPTPKSESDEPLLNLLRALSGIKRLELHNSTTKWLFAAPILHFPEFHYLLHLKLKYPPFNSTFLFDVLQKCPMLETLIAFSFDIDMVDRSFDSLPSYRWEAKSKSVPKCLISHLTFIHFYGCPENELEFIGYILQHGLVLKTMIVDEYWRDQPDRWMKKISDLPRGSAMCQVKFL