AlfalfaGEDB | MS.gene49513

M. sativa cultivar XinJiangDaYe / MS.gene49513

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF08387.13 PF12937.10

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49513.t1	XP_013446248.1	92	440	33	1	1	438	1	440	2.90E-234	820.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49513.t1	Q9FM89	26.8	441	276	15	15	432	6	422	1.0e-22	109.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49513.t1	A0A072U383	92.0	440	33	1	1	438	1	440	2.1e-234	820.8

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene03750	MS.gene49513	PPI
MS.gene03752	MS.gene49513	PPI
MS.gene055880	MS.gene49513	PPI
MS.gene055883	MS.gene49513	PPI
MS.gene065234	MS.gene49513	PPI
MS.gene065516	MS.gene49513	PPI
MS.gene072547	MS.gene49513	PPI
MS.gene072985	MS.gene49513	PPI
MS.gene09360	MS.gene49513	PPI
MS.gene09362	MS.gene49513	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49513.t1	MTR_8g070950	92.045	440	33	1	1	438	1	440	0.0	805
MS.gene49513.t1	MTR_8g062010	62.416	447	134	11	9	436	3	434	3.20e-178	505
MS.gene49513.t1	MTR_8g062080	57.516	459	162	9	1	436	1	449	1.39e-170	486
MS.gene49513.t1	MTR_8g062310	59.624	426	152	8	15	437	25	433	1.85e-166	475
MS.gene49513.t1	MTR_8g058965	56.211	475	154	13	1	436	1	460	6.81e-161	462
MS.gene49513.t1	MTR_8g469020	55.240	458	162	9	15	437	7	456	1.37e-158	456
MS.gene49513.t1	MTR_8g062300	53.265	490	166	13	1	436	1	481	6.01e-154	445
MS.gene49513.t1	MTR_8g467790	56.915	376	118	7	15	376	148	493	3.41e-132	390
MS.gene49513.t1	MTR_4g050250	51.991	427	149	13	1	378	1	420	1.36e-125	371
MS.gene49513.t1	MTR_2g083840	42.797	472	215	19	1	432	1	457	2.58e-93	289

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene49513.t1	AT1G78750	24.841	471	291	17	2	429	5	455	6.60e-26	109
MS.gene49513.t1	AT1G78750	24.841	471	291	17	2	429	5	455	6.60e-26	109
MS.gene49513.t1	AT5G56420	26.471	442	278	15	14	432	5	422	2.37e-25	107
MS.gene49513.t1	AT5G56420	26.471	442	278	15	14	432	5	422	2.37e-25	107
MS.gene49513.t1	AT5G56420	26.471	442	278	15	14	432	5	422	2.37e-25	107
MS.gene49513.t1	AT5G56560	27.790	439	277	17	12	432	2	418	3.42e-24	104
MS.gene49513.t1	AT5G53840	26.991	452	271	20	10	429	13	437	4.13e-22	98.6
MS.gene49513.t1	AT1G78760	25.764	458	289	20	7	433	8	445	1.65e-21	97.1
MS.gene49513.t1	AT1G69630	26.166	493	248	23	3	429	6	448	9.83e-21	94.7
MS.gene49513.t1	AT2G26030	27.093	454	278	18	15	429	4	443	2.05e-20	93.6

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Find 111 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTTGCCAACGATGCCCA+TGG	0.622590	8.1:+33851926	None:intergenic
AACCATCACCGTTGAGAACT+TGG	0.381357	8.1:-33851400	MS.gene49513:CDS
AACTCTGGATAATTTAGAAC+AGG	0.400095	8.1:+33851666	None:intergenic
AAGATTGACTGACATACCAA+AGG	0.615806	8.1:-33851186	MS.gene49513:CDS
AAGCTCTTGGAGCTGAAGGA+AGG	0.556513	8.1:+33852008	None:intergenic
AATGTGAATATAAGTCAGGT+GGG	0.643217	8.1:+33851340	None:intergenic
ACAAGTACCGGAGGGAAATG+AGG	0.559875	8.1:+33852416	None:intergenic
ACACCTCTGGGAGCATCTCC+AGG	0.444775	8.1:-33852701	MS.gene49513:CDS
ACCATCACCGTTGAGAACTT+GGG	0.571797	8.1:-33851399	MS.gene49513:CDS
ACCGGCTCCCAAGTTCTCAA+CGG	0.652084	8.1:+33851392	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGTGAATATAAGTCAGGT+GGG	+	chr8.1:33852646-33852665	None:intergenic	30.0%
ATTCACATTGAAGGATATCA+AGG	-	chr8.1:33852657-33852676	MS.gene49513:CDS	30.0%
CTGACTTATATTCACATTGA+AGG	-	chr8.1:33852648-33852667	MS.gene49513:CDS	30.0%
CTTCAATGTGAATATAAGTC+AGG	+	chr8.1:33852650-33852669	None:intergenic	30.0%
GAGAATGTTGAACTTTGATA+TGG	+	chr8.1:33851806-33851825	None:intergenic	30.0%
TGAAATAAATTGCGAAACTC+TGG	+	chr8.1:33852335-33852354	None:intergenic	30.0%
TTATGAGAACTTGAAGCATA+TGG	+	chr8.1:33852418-33852437	None:intergenic	30.0%
AAGATTGACTGACATACCAA+AGG	-	chr8.1:33852797-33852816	MS.gene49513:CDS	35.0%
AGATTGACTGACATACCAAA+GGG	-	chr8.1:33852798-33852817	MS.gene49513:CDS	35.0%
ATGTACACGATAACAAGTGA+CGG	+	chr8.1:33852278-33852297	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	33851136	33852869	33851136	ID=MS.gene49513
chr8.1	mRNA	33851136	33852869	33851136	ID=MS.gene49513.t1;Parent=MS.gene49513
chr8.1	exon	33852424	33852869	33852424	ID=MS.gene49513.t1.exon1;Parent=MS.gene49513.t1
chr8.1	CDS	33852424	33852869	33852424	ID=cds.MS.gene49513.t1;Parent=MS.gene49513.t1
chr8.1	exon	33851791	33852220	33851791	ID=MS.gene49513.t1.exon2;Parent=MS.gene49513.t1
chr8.1	CDS	33851791	33852220	33851791	ID=cds.MS.gene49513.t1;Parent=MS.gene49513.t1
chr8.1	exon	33851554	33851703	33851554	ID=MS.gene49513.t1.exon3;Parent=MS.gene49513.t1
chr8.1	CDS	33851554	33851703	33851554	ID=cds.MS.gene49513.t1;Parent=MS.gene49513.t1
chr8.1	exon	33851136	33851426	33851136	ID=MS.gene49513.t1.exon4;Parent=MS.gene49513.t1
chr8.1	CDS	33851136	33851426	33851136	ID=cds.MS.gene49513.t1;Parent=MS.gene49513.t1

Gene Sequence

>MS.gene49513

ATGGCGGAGTCAAGTTTGAAACGGCGGAAAGCAGAAGAAGAGGACCGATTGAGTAGTTTACCGGACTCCATTCTATGCCATATACTCTCCTTCCTCCCAACTCGAACCTCCGTCACAATTATGACTCTCGTCTCTCACAGGTGGCGACACCTCTGGGAGCATCTCCAGGTCTTCGACTTTGACGACTGGGATCCTCATTTTTACGACAAAAGTTTCGAAAAGTTTGCATCGTTCGTCAATGCTGTGCTCACTCTCCGTAGATCCCGCAACATTCGTAAGTTCCGTCTCAACTGTGATTACCCGGATCCCAAAATGTTTGAGCTGTGGTTCCGTGCTGCCACTGGTCCCCACCTTCAAGAACTCTCTCTCACCGTTCTAAACGTCGATGATTTGGTTAAACTCCCTCCTTCCCTCTTCATCAACTGCAACAACCTCATTTCCCTCCGGTACTTGTTTCAAATTTGTTTTTATTTCTATGAAACTGTCGAAATACACTTGTTTTAATGGTGGAAATGAAATTTGTTATCGATTTTACATATTATTATATCGATGCTCACTTCCATAATTTCATCTCTTGTTCCCTTTCTACTTCCAACACTGAAACAAATAAAGAATGTTATATATAACCTGTCTTTTGTTAATACTGCAGTCTGCATGGTGTACAAGTCCATATCAAAGTTCAACATTCTCCACTTCATTTTCCATCACTCAAGAGACTCAAACTTGGTGTAGAGACTGTGTATCCGGTAGTTGCTTTTCTCTCTGCCTGCCCTGTGCTGGAAACTATGGATATTAACTTTTTCCTTGAAGACATATCTTTGAACGAAGTTCCCATTCCACCTTCCTTCAGCTCCAAGAGCTTGAAATCCACCAGTGACAATTTCACTTGGACTCACTTTAAGTTTGATATATATCCCCATGCCATGGGCATCGTTGGCAACTTTCATAGTATGGTGGAAGCATTTCTCGATGTCTTTTCCTCACGTGAAAGTGAATTTGTCGACCCTATTCTCAACAACCTCTTCCAAGATAACAAAAACAATGAAATAAACCTACTTTCGCGTCATTTGACATCAAAGGTGAAGTTTTATTTTTATGGTTTGTATTTTTTTATATATGTAGTTGGTACTAATACTGCGCCGTCACTTGTTATCGTGTACATGCAGTTGCCACTTCACGCCCCTGTTCTAAATTATCCAGAGTTTCGCAATTTATTTCATCTAAAGTTCATTCTTCCCTGCTTCAACTCGAATCTTCTGGTCGACGTGCTTGAAAAATGCCATATGCTTCAAGTTCTCATAATTAAGAGCAGCAAGGTTTGTTGACTTCTCAAGTTTAATTTAATCAATATTTGGTATGTCTATATGTGCATGAATGTATGAATCTATTTGTGCATACAAATATATTTTTGAGTTGTGGCACGGTGTCCATTGTTACTTGTAGGAGGAACCATCACCGTTGAGAACTTGGGAGCCGGTGTCAACAACAGTTTCTGAATGTCTCAAATCCCACCTGACTTATATTCACATTGAAGGATATCAAGGATTTGAAGATGAGCTGACATTTGCTGAATATATTTTGCGGAACGGACTTGTTTTGAAGACAATGCTTATTTTTGTTGATATTTCAATGGACAAAACAAACAAGTATTGTTCTCTCAAAAGATTGACTGACATACCAAAGGGTTCTGTGTCATGCGAACTTAAATTTGACCCAGCTCCGTCTCCAAATTAA

Protein sequence

>MS.gene49513.t1

MAESSLKRRKAEEEDRLSSLPDSILCHILSFLPTRTSVTIMTLVSHRWRHLWEHLQVFDFDDWDPHFYDKSFEKFASFVNAVLTLRRSRNIRKFRLNCDYPDPKMFELWFRAATGPHLQELSLTVLNVDDLVKLPPSLFINCNNLISLRLHGVQVHIKVQHSPLHFPSLKRLKLGVETVYPVVAFLSACPVLETMDINFFLEDISLNEVPIPPSFSSKSLKSTSDNFTWTHFKFDIYPHAMGIVGNFHSMVEAFLDVFSSRESEFVDPILNNLFQDNKNNEINLLSRHLTSKLPLHAPVLNYPEFRNLFHLKFILPCFNSNLLVDVLEKCHMLQVLIIKSSKEEPSPLRTWEPVSTTVSECLKSHLTYIHIEGYQGFEDELTFAEYILRNGLVLKTMLIFVDISMDKTNKYCSLKRLTDIPKGSVSCELKFDPAPSPN