AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63185

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63185.t1 MTR_8g469020 92.544 456 34 0 1 456 1 456 0.0 864
MS.gene63185.t1 MTR_8g058965 66.000 450 150 2 7 455 13 460 0.0 580
MS.gene63185.t1 MTR_4g050250 64.109 404 132 7 5 397 19 420 7.02e-171 487
MS.gene63185.t1 MTR_8g070950 55.675 467 148 10 7 456 15 439 4.71e-160 460
MS.gene63185.t1 MTR_8g062010 53.712 458 171 7 7 455 9 434 9.16e-152 438
MS.gene63185.t1 MTR_8g062080 52.026 469 173 13 2 455 18 449 8.12e-145 421
MS.gene63185.t1 MTR_8g062310 52.863 454 165 11 7 456 25 433 4.42e-142 414
MS.gene63185.t1 MTR_8g062300 47.541 488 185 11 7 455 26 481 4.01e-128 380
MS.gene63185.t1 MTR_2g083840 43.929 453 234 7 5 451 19 457 5.14e-102 312
MS.gene63185.t1 MTR_8g467790 46.269 402 147 13 7 395 148 493 1.85e-99 307
MS.gene63185.t1 MTR_4g032730 40.506 474 230 12 7 450 15 466 6.16e-95 294
MS.gene63185.t1 MTR_8g468810 41.901 463 227 16 7 451 25 463 3.76e-94 292
MS.gene63185.t1 MTR_8g058970 41.929 477 227 17 7 451 19 477 1.61e-93 291
MS.gene63185.t1 MTR_8g062270 39.651 459 241 11 7 450 16 453 1.96e-87 275
MS.gene63185.t1 MTR_8g468840 40.708 452 242 9 7 449 14 448 7.53e-87 273
MS.gene63185.t1 MTR_8g467800 42.060 466 227 12 7 456 20 458 1.53e-86 272
MS.gene63185.t1 MTR_5g092960 38.478 460 250 8 7 450 19 461 3.31e-82 261
MS.gene63185.t1 MTR_8g014260 39.868 454 246 15 7 451 16 451 1.64e-78 251
MS.gene63185.t1 MTR_8g468810 41.779 371 176 14 7 361 25 371 2.08e-68 223
MS.gene63185.t1 MTR_8g468760 37.908 459 221 13 7 450 9 418 2.26e-68 224
MS.gene63185.t1 MTR_8g445780 39.409 406 210 13 7 404 13 390 2.96e-64 212
MS.gene63185.t1 MTR_2g086020 69.863 146 44 0 311 456 175 320 3.09e-64 210
MS.gene63185.t1 MTR_2g086020 46.358 151 65 4 34 178 2 142 3.51e-30 119
MS.gene63185.t1 MTR_8g014160 38.178 461 205 20 1 451 35 425 8.65e-64 212
MS.gene63185.t1 MTR_8g468810 40.979 327 161 11 7 324 25 328 1.26e-58 196
MS.gene63185.t1 MTR_8g468810 42.357 314 149 11 7 311 25 315 1.30e-58 196
MS.gene63185.t1 MTR_8g468810 42.357 314 149 11 7 311 25 315 2.68e-58 196
MS.gene63185.t1 MTR_5g092960 38.147 367 201 6 7 361 19 371 2.78e-56 192
MS.gene63185.t1 MTR_4g024710 39.159 309 163 12 149 451 58 347 7.56e-44 157
MS.gene63185.t1 MTR_8g467800 41.801 311 154 9 7 311 20 309 1.51e-43 156
MS.gene63185.t1 MTR_8g014210 39.916 238 131 8 219 451 1 231 1.60e-37 137
MS.gene63185.t1 MTR_4g024745 38.194 288 155 12 147 429 10 279 3.57e-35 132
MS.gene63185.t1 MTR_5g021550 51.370 146 65 6 7 148 19 162 3.36e-33 123
MS.gene63185.t1 MTR_1g041245 28.970 466 226 19 4 451 8 386 1.92e-32 127
MS.gene63185.t1 MTR_8g077460 45.833 144 74 4 309 451 235 375 2.33e-30 121
MS.gene63185.t1 MTR_8g077460 38.462 234 122 7 7 234 10 227 2.42e-30 121
MS.gene63185.t1 MTR_5g080710 28.764 445 278 16 7 443 33 446 5.95e-29 119
MS.gene63185.t1 MTR_5g084070 27.517 447 276 15 6 443 12 419 1.05e-28 117
MS.gene63185.t1 MTR_3g099800 56.322 87 37 1 363 448 19 105 5.20e-27 104
MS.gene63185.t1 MTR_4g024720 48.246 114 53 4 7 116 17 128 4.86e-24 99.8
MS.gene63185.t1 MTR_7g056433 26.211 454 254 15 5 451 10 389 9.67e-24 102
MS.gene63185.t1 MTR_6g022500 27.766 461 232 17 7 451 101 476 1.07e-23 103
MS.gene63185.t1 MTR_8g014290 42.208 154 75 5 7 149 10 160 6.00e-23 95.5
MS.gene63185.t1 MTR_5g008950 25.439 456 236 17 9 451 1 365 1.06e-21 96.7
MS.gene63185.t1 MTR_7g026500 26.350 463 254 17 2 452 9 396 3.02e-20 92.8
MS.gene63185.t1 MTR_2g036470 26.247 461 248 19 5 450 12 395 6.63e-20 91.7
MS.gene63185.t1 MTR_3g023630 25.934 455 237 19 5 448 6 371 2.65e-19 89.7
MS.gene63185.t1 MTR_7g032370 24.138 435 244 15 19 451 2 352 5.06e-19 88.6
MS.gene63185.t1 MTR_7g033915 25.758 462 223 18 4 450 8 364 5.32e-19 88.6
MS.gene63185.t1 MTR_7g033990 26.292 445 248 15 5 445 9 377 7.18e-19 89.0
MS.gene63185.t1 MTR_3g105060 24.000 450 253 15 4 451 11 373 1.05e-18 87.8
MS.gene63185.t1 MTR_5g045170 27.051 451 240 20 9 448 16 388 1.88e-18 87.4
MS.gene63185.t1 MTR_3g087260 26.572 493 265 15 7 450 12 456 4.54e-18 86.7
MS.gene63185.t1 MTR_3g108150 31.481 216 116 9 1 213 7 193 2.42e-17 84.0
MS.gene63185.t1 MTR_7g056447 31.905 210 110 8 4 209 6 186 3.46e-17 82.4
MS.gene63185.t1 MTR_1g038710 27.126 435 270 16 7 419 19 428 4.49e-17 83.6
MS.gene63185.t1 MTR_7g032380 31.905 210 116 8 4 211 8 192 2.35e-16 80.5
MS.gene63185.t1 MTR_7g032380 31.210 157 94 4 301 451 208 356 7.47e-12 67.0
MS.gene63185.t1 MTR_2g007500 30.047 213 111 7 2 211 9 186 4.45e-16 79.7
MS.gene63185.t1 MTR_7g088260 24.276 449 259 18 7 451 91 462 1.07e-15 79.3
MS.gene63185.t1 MTR_1g108820 28.062 449 249 21 4 448 5 383 1.37e-15 78.6
MS.gene63185.t1 MTR_8g014230 42.857 84 46 2 361 444 17 98 1.60e-15 72.8
MS.gene63185.t1 MTR_4g056040 26.667 465 258 19 7 419 9 442 3.54e-15 78.2
MS.gene63185.t1 MTR_5g073420 30.986 213 118 9 3 210 7 195 6.27e-15 76.6
MS.gene63185.t1 MTR_8g085430 29.817 218 117 9 4 211 8 199 1.16e-14 75.1
MS.gene63185.t1 MTR_6g011460 32.444 225 122 9 7 227 46 244 1.33e-14 75.9
MS.gene63185.t1 MTR_3g107160 34.906 212 111 9 7 214 10 198 1.56e-14 75.5
MS.gene63185.t1 MTR_3g099830 25.751 466 220 21 5 448 10 371 3.25e-14 74.3
MS.gene63185.t1 MTR_5g008920 32.227 211 112 8 5 211 7 190 3.79e-14 74.3
MS.gene63185.t1 MTR_4g056030 33.010 206 117 8 7 211 9 194 4.21e-14 74.7
MS.gene63185.t1 MTR_2g007400 24.043 470 260 16 7 454 12 406 5.00e-14 73.9
MS.gene63185.t1 MTR_4g098590 24.171 422 240 13 9 418 1 354 1.13e-13 72.8
MS.gene63185.t1 MTR_7g115270 29.545 220 122 8 4 214 9 204 1.36e-13 72.8
MS.gene63185.t1 MTR_7g055943 32.857 210 111 10 5 210 38 221 1.57e-13 72.8
MS.gene63185.t1 MTR_4g080773 24.059 478 282 17 7 447 19 452 2.01e-13 72.4
MS.gene63185.t1 MTR_7g034110 29.767 215 120 9 4 213 8 196 2.03e-13 71.6
MS.gene63185.t1 MTR_4g080773 24.059 478 282 17 7 447 19 452 2.47e-13 72.4
MS.gene63185.t1 MTR_4g080590 24.766 428 277 15 7 419 23 420 3.56e-13 71.6
MS.gene63185.t1 MTR_1g053025 25.328 458 253 20 7 448 2 386 3.72e-13 71.2
MS.gene63185.t1 MTR_7g098570 30.702 228 113 11 7 222 20 214 5.07e-13 71.2
MS.gene63185.t1 MTR_4g039440 26.809 470 230 21 6 451 33 412 7.35e-13 70.5
MS.gene63185.t1 MTR_5g087940 33.484 221 115 10 7 220 26 221 2.03e-12 68.9
MS.gene63185.t1 MTR_5g058100 30.415 217 120 8 2 214 4 193 2.03e-12 68.9
MS.gene63185.t1 MTR_2g082880 24.408 422 269 14 7 413 10 396 3.30e-12 68.6
MS.gene63185.t1 MTR_7g021630 27.304 293 170 10 7 289 17 276 3.57e-12 68.2
MS.gene63185.t1 MTR_7g021630 27.304 293 170 10 7 289 17 276 4.05e-12 67.8
MS.gene63185.t1 MTR_2g100590 25.455 385 224 14 5 378 40 372 4.06e-12 68.2
MS.gene63185.t1 MTR_3g107120 25.108 462 252 17 7 452 9 392 4.36e-12 68.2
MS.gene63185.t1 MTR_2g100590 25.455 385 224 14 5 378 40 372 4.73e-12 68.2
MS.gene63185.t1 MTR_3g115430 37.838 111 63 3 342 451 20 125 6.08e-12 63.2
MS.gene63185.t1 MTR_5g058000 29.756 205 112 8 7 207 8 184 6.72e-12 67.4
MS.gene63185.t1 MTR_2g100590 25.455 385 224 14 5 378 40 372 6.78e-12 67.8
MS.gene63185.t1 MTR_6g022360 31.068 206 103 7 7 210 43 211 1.05e-11 67.0
MS.gene63185.t1 MTR_0543s0010 30.660 212 114 10 1 211 80 259 1.09e-11 67.0
MS.gene63185.t1 MTR_2g007450 29.493 217 124 7 7 210 16 216 1.22e-11 66.6
MS.gene63185.t1 MTR_3g107100 32.524 206 115 8 7 211 11 193 1.23e-11 66.6
MS.gene63185.t1 MTR_0492s0020 30.660 212 114 10 1 211 80 259 1.30e-11 66.6
MS.gene63185.t1 MTR_7g075820 28.440 218 129 6 4 208 3 206 1.41e-11 66.2
MS.gene63185.t1 MTR_7g034115 27.490 251 152 11 4 251 8 231 1.86e-11 65.9
MS.gene63185.t1 MTR_7g007340 28.959 221 93 10 3 211 4 172 2.32e-11 65.5
MS.gene63185.t1 MTR_6g022390 34.921 126 74 4 7 126 5 128 3.09e-11 62.0
MS.gene63185.t1 MTR_3g036250 29.665 209 118 8 6 210 4 187 6.50e-11 64.3
MS.gene63185.t1 MTR_3g110010 33.495 206 108 10 7 211 13 190 6.69e-11 65.1
MS.gene63185.t1 MTR_3g109982 33.971 209 105 11 7 212 13 191 8.33e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63185.t1 AT4G10400 29.167 456 262 20 7 450 2 408 2.80e-25 107
MS.gene63185.t1 AT4G10400 29.167 456 262 20 7 450 2 408 2.80e-25 107
MS.gene63185.t1 AT4G10400 29.167 456 262 20 7 450 2 408 2.80e-25 107
MS.gene63185.t1 AT5G56420 27.430 463 272 18 7 451 6 422 6.59e-24 103
MS.gene63185.t1 AT5G56420 27.430 463 272 18 7 451 6 422 6.59e-24 103
MS.gene63185.t1 AT5G56420 27.430 463 272 18 7 451 6 422 6.59e-24 103
MS.gene63185.t1 AT1G16930 27.027 481 273 18 5 451 13 449 8.38e-23 100
MS.gene63185.t1 AT1G16930 27.027 481 273 18 5 451 13 449 8.38e-23 100
MS.gene63185.t1 AT1G16930 27.027 481 273 18 5 451 13 449 8.38e-23 100
MS.gene63185.t1 AT4G26340 27.426 474 259 18 7 451 2 419 1.11e-22 100
MS.gene63185.t1 AT4G09920 33.624 229 127 8 7 233 2 207 5.88e-22 95.9
MS.gene63185.t1 AT4G09920 33.624 229 127 8 7 233 2 207 5.88e-22 95.9
MS.gene63185.t1 AT4G09920 33.624 229 127 8 7 233 2 207 5.88e-22 95.9
MS.gene63185.t1 AT4G09920 34.348 230 124 9 7 233 2 207 7.00e-22 96.3
MS.gene63185.t1 AT4G09920 34.348 230 124 9 7 233 2 207 7.00e-22 96.3
MS.gene63185.t1 AT5G53840 27.802 464 277 18 7 452 18 441 1.70e-21 97.1
MS.gene63185.t1 AT3G42770 27.876 452 274 14 9 443 1 417 2.57e-19 90.9
MS.gene63185.t1 AT4G26350 26.483 472 275 20 7 451 2 428 2.98e-19 90.1
MS.gene63185.t1 AT5G22730 24.576 472 290 17 7 448 28 463 4.91e-19 89.7
MS.gene63185.t1 AT5G22730 24.576 472 290 17 7 448 28 463 4.91e-19 89.7
MS.gene63185.t1 AT3G59200 33.023 215 109 8 5 210 5 193 1.51e-18 87.4
MS.gene63185.t1 AT1G69630 25.265 471 285 18 6 448 17 448 2.67e-18 87.4
MS.gene63185.t1 AT3G59200 32.243 214 112 7 5 210 5 193 3.73e-18 87.4
MS.gene63185.t1 AT5G22720 23.958 480 291 14 7 450 23 464 6.16e-18 86.3
MS.gene63185.t1 AT1G06630 27.419 372 225 13 7 361 12 355 8.06e-18 85.1
MS.gene63185.t1 AT1G06630 27.419 372 225 13 7 361 12 355 8.06e-18 85.1
MS.gene63185.t1 AT1G06630 30.000 230 142 6 7 233 12 225 1.13e-17 84.0
MS.gene63185.t1 AT1G06630 27.371 369 223 13 7 358 12 352 2.47e-17 84.0
MS.gene63185.t1 AT1G06630 27.371 369 223 13 7 358 12 352 2.47e-17 84.0
MS.gene63185.t1 AT1G06630 27.371 369 223 13 7 358 12 352 2.47e-17 84.0
MS.gene63185.t1 AT1G06630 27.371 369 223 13 7 358 12 352 2.47e-17 84.0
MS.gene63185.t1 AT2G26860 28.571 427 242 16 7 410 2 388 2.65e-17 84.0
MS.gene63185.t1 AT2G26860 28.571 427 242 16 7 410 2 388 2.65e-17 84.0
MS.gene63185.t1 AT4G13985 24.477 478 287 18 6 448 18 456 2.82e-17 84.3
MS.gene63185.t1 AT1G06630 28.369 282 170 9 7 279 21 279 2.90e-17 84.0
MS.gene63185.t1 AT5G44950 26.190 462 280 22 7 442 4 430 3.44e-17 84.0
MS.gene63185.t1 AT1G06630 27.371 369 223 13 7 358 12 352 4.76e-17 83.6
MS.gene63185.t1 AT5G56400 24.731 465 292 16 5 451 31 455 7.23e-17 83.2
MS.gene63185.t1 AT5G60610 25.882 425 256 16 7 415 2 383 9.40e-17 82.4
MS.gene63185.t1 AT5G60610 25.882 425 256 16 7 415 2 383 9.40e-17 82.4
MS.gene63185.t1 AT1G32375 26.042 480 261 24 7 451 2 422 1.37e-16 82.0
MS.gene63185.t1 AT1G32375 26.042 480 261 24 7 451 2 422 1.37e-16 82.0
MS.gene63185.t1 AT4G00315 26.752 471 286 18 7 450 2 440 2.01e-16 81.6
MS.gene63185.t1 AT5G56560 26.201 458 279 17 8 451 6 418 2.27e-16 81.3
MS.gene63185.t1 AT1G55660 26.407 462 299 16 7 450 53 491 2.57e-16 81.6
MS.gene63185.t1 AT3G44060 25.054 459 292 14 9 450 1 424 4.45e-16 80.5
MS.gene63185.t1 AT3G44810 32.800 250 138 12 1 240 1 230 9.62e-16 79.7
MS.gene63185.t1 AT4G00160 27.426 474 274 20 7 453 16 446 1.08e-15 79.3
MS.gene63185.t1 AT5G50270 26.914 431 258 15 7 418 2 394 1.47e-15 79.0
MS.gene63185.t1 AT5G56440 33.824 204 112 7 7 210 2 182 1.52e-15 79.0
MS.gene63185.t1 AT3G60040 27.103 428 237 18 5 400 11 395 2.19e-15 79.0
MS.gene63185.t1 AT1G51370 25.478 471 261 21 7 446 19 430 2.22e-15 78.6
MS.gene63185.t1 AT5G56690 27.990 418 232 19 9 399 4 379 2.48e-15 78.2
MS.gene63185.t1 AT3G60040 27.103 428 237 18 5 400 230 614 2.65e-15 79.0
MS.gene63185.t1 AT3G60040 27.103 428 237 18 5 400 231 615 2.67e-15 79.0
MS.gene63185.t1 AT3G60040 27.103 428 237 18 5 400 228 612 2.84e-15 78.6
MS.gene63185.t1 AT3G60040 27.103 428 237 18 5 400 230 614 2.95e-15 78.6
MS.gene63185.t1 AT1G05080 25.431 464 288 16 7 450 13 438 7.42e-15 76.6
MS.gene63185.t1 AT3G59190 33.889 180 106 5 1 175 6 177 1.18e-14 75.9
MS.gene63185.t1 AT3G59190 33.889 180 106 5 1 175 6 177 1.54e-14 76.3
MS.gene63185.t1 AT5G22610 23.890 473 286 18 7 455 18 440 1.63e-14 75.9
MS.gene63185.t1 AT1G13780 24.370 476 299 19 4 448 7 452 1.96e-14 75.5
MS.gene63185.t1 AT5G53635 25.370 473 273 20 9 450 2 425 2.64e-14 75.1
MS.gene63185.t1 AT4G13965 26.840 462 269 23 6 448 9 420 2.98e-14 74.7
MS.gene63185.t1 AT5G56370 24.520 469 279 17 7 450 2 420 5.50e-14 73.9
MS.gene63185.t1 AT5G56370 24.520 469 279 17 7 450 2 420 5.50e-14 73.9
MS.gene63185.t1 AT5G22610 23.299 485 304 18 7 455 18 470 6.10e-14 74.3
MS.gene63185.t1 AT5G56410 24.944 449 245 14 14 418 10 410 9.80e-14 73.6
MS.gene63185.t1 AT5G22670 26.009 446 244 19 5 413 9 405 3.00e-13 72.0
MS.gene63185.t1 AT4G15060 32.524 206 115 8 7 210 26 209 3.00e-13 71.6
MS.gene63185.t1 AT1G66310 26.767 467 273 22 6 448 18 439 3.35e-13 71.6
MS.gene63185.t1 AT4G15060 33.010 206 114 8 7 210 172 355 3.71e-13 72.0
MS.gene63185.t1 AT4G14103 29.707 239 136 9 7 240 8 219 4.13e-13 71.2
MS.gene63185.t1 AT3G58920 25.100 498 289 19 7 451 2 468 5.54e-13 71.2
MS.gene63185.t1 AT4G14103 29.461 241 138 9 5 240 6 219 5.66e-13 70.5
MS.gene63185.t1 AT4G14103 29.461 241 138 9 5 240 6 219 5.66e-13 70.5
MS.gene63185.t1 AT3G52680 27.479 484 259 19 7 453 21 449 5.98e-13 70.9
MS.gene63185.t1 AT5G38590 24.454 458 260 19 7 436 2 401 3.43e-12 68.6
MS.gene63185.t1 AT5G22660 24.375 480 276 19 7 448 13 443 3.78e-12 68.6
MS.gene63185.t1 AT3G52680 33.010 206 111 7 7 210 21 201 1.16e-11 66.6
MS.gene63185.t1 AT1G51370 30.097 206 109 9 7 210 19 191 1.73e-11 65.9
MS.gene63185.t1 AT5G62970 24.370 476 284 17 14 450 10 448 1.77e-11 66.2
MS.gene63185.t1 AT1G51370 30.097 206 109 9 7 210 19 191 1.95e-11 66.2
MS.gene63185.t1 AT2G42720 30.622 209 120 8 7 210 2 190 2.31e-11 65.9
MS.gene63185.t1 AT5G22730 25.066 379 225 15 7 357 28 375 2.88e-11 65.5
MS.gene63185.t1 AT5G41840 29.032 217 125 10 5 210 12 210 3.58e-11 65.9
MS.gene63185.t1 AT5G18780 24.353 464 293 15 7 448 11 438 4.24e-11 65.1
MS.gene63185.t1 AT5G18780 24.353 464 293 15 7 448 11 438 4.24e-11 65.1
MS.gene63185.t1 AT3G26922 26.296 270 134 10 7 243 14 251 5.41e-11 63.9
MS.gene63185.t1 AT3G26922 26.296 270 134 10 7 243 14 251 5.41e-11 63.9
MS.gene63185.t1 AT5G38590 27.211 294 151 13 7 279 2 253 7.83e-11 63.2

Find 115 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGCTCCCATGTTCTTAA+AGG 0.198525 8.4:-41042962 None:intergenic
TGATGTAAATTGCAAAATTC+TGG 0.267891 8.4:-41042720 None:intergenic
TAGAACAGTTCTTGTTAGTT+AGG 0.297797 8.4:-41043141 None:intergenic
TTGGGAGGAAGGAGAGTATA+TGG 0.316717 8.4:-41040791 None:intergenic
CGTCGTCGCCACGGAGGTTC+TGG 0.328724 8.4:-41040814 None:intergenic
TCATTACTTGTCTTTCTCTC+TGG 0.347561 8.4:+41042252 MS.gene63185:CDS
CTCAGCTACCTCCGAAATAA+TGG 0.349487 8.4:+41042540 MS.gene63185:CDS
TTCAAGAGGTTGAAATTAAC+CGG 0.370371 8.4:+41042348 MS.gene63185:CDS
CTGACCTTTATTCACATAGA+AGG 0.373907 8.4:+41043019 MS.gene63185:CDS
GATGTATATCACCATTATTT+CGG 0.382996 8.4:-41042551 None:intergenic
TAACCGGCGTAAATTTCTCT+TGG 0.385560 8.4:+41042364 MS.gene63185:CDS
GTAGCGATTGCTTCGAGGTA+TGG 0.388778 8.4:+41041039 MS.gene63185:CDS
GTATGGGTCCGTGCTGCCAT+TGG 0.397086 8.4:+41041056 MS.gene63185:CDS
AATTCTGGATAATTTAGAAC+AGG 0.399159 8.4:-41042705 None:intergenic
ATATCCTTCTATGTGAATAA+AGG 0.410094 8.4:-41043023 None:intergenic
CCTCCGTGAAAGTGAATTTG+TGG 0.417614 8.4:+41042509 MS.gene63185:CDS
AAAACCACATTGGGCATCAT+TGG 0.426834 8.4:+41042444 MS.gene63185:CDS
TATGGGTCCGTGCTGCCATT+GGG 0.427675 8.4:+41041057 MS.gene63185:CDS
TTGAATACTGTGATCAAGAT+TGG 0.431394 8.4:+41041108 MS.gene63185:CDS
GATTCAGCTGTGTCTCCATA+AGG 0.432456 8.4:+41043217 MS.gene63185:CDS
TAGCGATTGCTTCGAGGTAT+GGG 0.441737 8.4:+41041040 MS.gene63185:CDS
GCAAACCTTCTGAACAATCT+TGG 0.441940 8.4:-41040924 None:intergenic
AGAGTATATGGAAGAGGAGT+GGG 0.452182 8.4:-41040779 None:intergenic
GCACCCATCTCGTTTCCCTT+AGG 0.453151 8.4:+41041162 MS.gene63185:CDS
ATTCACATAGAAGGATATCA+AGG 0.454447 8.4:+41043028 MS.gene63185:CDS
ATATGGAAGAGGAGTGGGTC+TGG 0.454721 8.4:-41040774 None:intergenic
TCCCATGTTCTTAAAGGTGA+TGG 0.457043 8.4:-41042956 None:intergenic
GTAGGAATTGTTGTTGACTT+TGG 0.458815 8.4:-41042980 None:intergenic
CAGCTGAATCAAATTTAAGT+TGG 0.465585 8.4:-41043204 None:intergenic
ACTCACACGAAGACGGAAAA+CGG 0.467859 8.4:+41040901 MS.gene63185:CDS
AATTTCAACCTCTTGAAGAA+AGG 0.468007 8.4:-41042342 None:intergenic
TCCGTCTTCGTGTGAGTCGT+CGG 0.468119 8.4:-41040895 None:intergenic
CTCGCAGGTGGCGAAATCTC+TGG 0.470853 8.4:+41040847 MS.gene63185:CDS
AGTTTCAACATCTTCAATGA+TGG 0.471452 8.4:-41042162 None:intergenic
GTTTCGTAGCGATTGCTTCG+AGG 0.472167 8.4:+41041034 MS.gene63185:CDS
CCGTGTCACCTTTCTTCAAG+AGG 0.472204 8.4:+41042334 MS.gene63185:CDS
GTCGGTGGTCGGCGGCGCCA+TGG 0.472690 8.4:-41040736 None:intergenic
CAGAAGACTCATGTTGAAGC+AGG 0.473912 8.4:-41042760 None:intergenic
GTCGCCACGGAGGTTCTGGT+TGG 0.481028 8.4:-41040810 None:intergenic
AGCACGGGCAAAACCACATT+GGG 0.482216 8.4:+41042435 MS.gene63185:CDS
GGAAGGAGAGTATATGGAAG+AGG 0.484769 8.4:-41040785 None:intergenic
GGAGGTTCTGGTTGGGAGGA+AGG 0.491388 8.4:-41040802 None:intergenic
CTTCTATGTGAATAAAGGTC+AGG 0.500493 8.4:-41043018 None:intergenic
AACGGCCAAGATTGTTCAGA+AGG 0.503657 8.4:+41040919 MS.gene63185:CDS
ATCTTGATCACAGTATTCAA+TGG 0.506654 8.4:-41041105 None:intergenic
TTTCGGAGGTAGCTGAGCAC+AGG 0.510489 8.4:-41042534 None:intergenic
CAATCTAGAGTTTCGAGTAC+GGG 0.517008 8.4:-41042279 None:intergenic
GTCTTCAAATAGATTCTGAC+TGG 0.517364 8.4:+41042391 MS.gene63185:CDS
GGTCCACAAATTCACTTTCA+CGG 0.521103 8.4:-41042512 None:intergenic
GAAAGGTGACACGGGAACAT+TGG 0.528334 8.4:-41042325 None:intergenic
TCGCCACGGAGGTTCTGGTT+GGG 0.531406 8.4:-41040809 None:intergenic
TCAAGGATTTGAAGATGAGC+TGG 0.532840 8.4:+41043045 MS.gene63185:CDS
TTCTTCAAGTTGAGGCCCAA+TGG 0.546289 8.4:-41041072 None:intergenic
TGGTAGAGTGCTTAATCTGT+CGG 0.552734 8.4:-41040754 None:intergenic
GAGAGTATATGGAAGAGGAG+TGG 0.553915 8.4:-41040780 None:intergenic
AACCATCACCTTTAAGAACA+TGG 0.553947 8.4:+41042954 MS.gene63185:CDS
GCAATCTAGAGTTTCGAGTA+CGG 0.554117 8.4:-41042280 None:intergenic
CTTACCTAAGGGAAACGAGA+TGG 0.555855 8.4:-41041166 None:intergenic
CACAAATTCACTTTCACGGA+GGG 0.558062 8.4:-41042508 None:intergenic
GGCGTTGTGGAATTCGACAT+TGG 0.558444 8.4:+41042192 MS.gene63185:CDS
TATGCGTCATTTCACATCAA+AGG 0.563852 8.4:+41042578 MS.gene63185:CDS
GAAACTCGGCGCAGGCGTTG+TGG 0.568607 8.4:+41042179 MS.gene63185:CDS
TTACCTAAGGGAAACGAGAT+GGG 0.569257 8.4:-41041165 None:intergenic
CATTGGAAACTTGCAGAGTG+TGG 0.571216 8.4:+41042461 MS.gene63185:CDS
GTGCAATCAAGAAGAGAAGA+AGG 0.574566 8.4:-41041143 None:intergenic
GATGGAAGATGAATTGAAGA+AGG 0.577459 8.4:-41042144 None:intergenic
CTTAATCTGTCGGTGGTCGG+CGG 0.577807 8.4:-41040744 None:intergenic
GTGCTTAATCTGTCGGTGGT+CGG 0.579525 8.4:-41040747 None:intergenic
CGACGAGCCTCATCTCTCGC+AGG 0.580759 8.4:+41040832 MS.gene63185:CDS
TAATGTCGCGGGATCGACGG+AGG 0.582154 8.4:-41040971 None:intergenic
GTGGTCTGTTGAATGATGAC+TGG 0.582340 8.4:+41041012 MS.gene63185:CDS
ACAAACAGTTCTTCAAGTTG+AGG 0.586776 8.4:-41041080 None:intergenic
AAGATGTTGAAACTCGGCGC+AGG 0.590475 8.4:+41042171 MS.gene63185:CDS
CCACGGAGGTTCTGGTTGGG+AGG 0.593707 8.4:-41040806 None:intergenic
CTATGTGAATAAAGGTCAGG+TGG 0.594985 8.4:-41043015 None:intergenic
ACCATCACCTTTAAGAACAT+GGG 0.597069 8.4:+41042955 MS.gene63185:CDS
CCTCTTGAAGAAAGGTGACA+CGG 0.598686 8.4:-41042334 None:intergenic
TCTCTTAATTCAGAGTAGCA+AGG 0.599848 8.4:+41042818 MS.gene63185:CDS
AAGATTAACTGATATACCAA+GGG 0.602286 8.4:+41043168 MS.gene63185:CDS
GTCATCATTCAACAGACCAC+TGG 0.602406 8.4:-41041009 None:intergenic
GTAGACGACAGTCGTAGCAC+GGG 0.604782 8.4:+41042420 MS.gene63185:CDS
TGTAGACGACAGTCGTAGCA+CGG 0.604863 8.4:+41042419 MS.gene63185:CDS
TAGCACGGGCAAAACCACAT+TGG 0.607098 8.4:+41042434 MS.gene63185:CDS
TCCGACGACTCACACGAAGA+CGG 0.609103 8.4:+41040894 MS.gene63185:CDS
TGGCATGTGACAGAGCCCCT+TGG 0.611510 8.4:-41043184 None:intergenic
CTCTTGAAGAAAGGTGACAC+GGG 0.612206 8.4:-41042333 None:intergenic
GATGAGGCTCGTCGTCGCCA+CGG 0.613003 8.4:-41040823 None:intergenic
GAAGATTAACTGATATACCA+AGG 0.613068 8.4:+41043167 MS.gene63185:CDS
AATGTCGCGGGATCGACGGA+GGG 0.613106 8.4:-41040970 None:intergenic
ATTCGACAACATAGACATTG+TGG 0.613283 8.4:+41042227 MS.gene63185:CDS
TTCGGAGGTAGCTGAGCACA+GGG 0.616465 8.4:-41042533 None:intergenic
AGAAGACTCATGTTGAAGCA+GGG 0.620873 8.4:-41042759 None:intergenic
GTATATCACCATTATTTCGG+AGG 0.623940 8.4:-41042548 None:intergenic
TTCTGGATAATTTAGAACAG+GGG 0.624941 8.4:-41042703 None:intergenic
GTTGAGGCCCAATGGCAGCA+CGG 0.625930 8.4:-41041064 None:intergenic
ATTCTGGATAATTTAGAACA+GGG 0.628560 8.4:-41042704 None:intergenic
AGGTGGGATTTGAAACATGT+AGG 0.629413 8.4:-41042998 None:intergenic
TTCGCCACCTGCGAGAGATG+AGG 0.637648 8.4:-41040839 None:intergenic
TATGTGAATAAAGGTCAGGT+GGG 0.640734 8.4:-41043014 None:intergenic
TCATTGAAGATGTTGAAACT+CGG 0.641916 8.4:+41042165 MS.gene63185:CDS
TTTAGAACAGGGGTGCGAAG+TGG 0.642724 8.4:-41042693 None:intergenic
TGAGTCGTCGGAGAATTCGA+AGG 0.644607 8.4:-41040883 None:intergenic
TGGAAACTTGCAGAGTGTGG+AGG 0.645099 8.4:+41042464 MS.gene63185:CDS
AATCTAGAGTTTCGAGTACG+GGG 0.649721 8.4:-41042278 None:intergenic
GTTTCCAATGATGCCCAATG+TGG 0.653602 8.4:-41042448 None:intergenic
TTAAGAGAACTTGAAGCACA+TGG 0.655061 8.4:-41042803 None:intergenic
TAGAGTGCTTAATCTGTCGG+TGG 0.663885 8.4:-41040751 None:intergenic
CCTCCCAACCAGAACCTCCG+TGG 0.670176 8.4:+41040806 MS.gene63185:CDS
CGAGCCTCATCTCTCGCAGG+TGG 0.671427 8.4:+41040835 MS.gene63185:CDS
GAGGCTCGTCGTCGCCACGG+AGG 0.682787 8.4:-41040820 None:intergenic
AGTCCAAGAGAAATTTACGC+CGG 0.686917 8.4:-41042367 None:intergenic
GTTTGCATTCTTCGTCAACG+CGG 0.698485 8.4:+41040941 MS.gene63185:CDS
ATTCGACATTGGCAACACTG+TGG 0.711162 8.4:+41042203 MS.gene63185:CDS
CCACAAATTCACTTTCACGG+AGG 0.721101 8.4:-41042509 None:intergenic
AGATTAACTGATATACCAAG+GGG 0.721766 8.4:+41043169 MS.gene63185:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GCTTTATTTAAATTAATTTT+TGG + chr8.4:41042844-41042863 MS.gene63185:intron 10.0%
!!! TTTTAATAATATCCAAAATA+TGG + chr8.4:41042000-41042019 MS.gene63185:intron 10.0%
!! AATGTTTATCTGTAAAAATT+AGG + chr8.4:41041269-41041288 MS.gene63185:intron 15.0%
!! GTGTAAAAAAAAAAAAAACA+AGG - chr8.4:41041800-41041819 None:intergenic 15.0%
!! TTGAAACTAAAAAAACTAAA+AGG + chr8.4:41041500-41041519 MS.gene63185:intron 15.0%
!!! ATTTTTGAAAGTATGTAATT+TGG + chr8.4:41041610-41041629 MS.gene63185:intron 15.0%
!!! TAGTTTTTTTAGTTTCAAAT+TGG - chr8.4:41041498-41041517 None:intergenic 15.0%
!!! TTTTTTTTTTTTTACACAAG+AGG + chr8.4:41041803-41041822 MS.gene63185:intron 15.0%
!! AACCTATAATACCAATAATA+AGG + chr8.4:41041303-41041322 MS.gene63185:intron 20.0%
!! GTTACAAAAAAGAAAATTAG+AGG + chr8.4:41041443-41041462 MS.gene63185:intron 20.0%
!! TACATACATTTCCTTATTAT+TGG - chr8.4:41041317-41041336 None:intergenic 20.0%
!! TGTATGTAAGTAATCATATT+TGG + chr8.4:41041329-41041348 MS.gene63185:intron 20.0%
!! TTCCTTATTATTGGTATTAT+AGG - chr8.4:41041308-41041327 None:intergenic 20.0%
!! TTTATGTTGGCAAATAAAAA+TGG + chr8.4:41041578-41041597 MS.gene63185:intron 20.0%
!!! AAGGTGAAGTTTTATTATTA+TGG + chr8.4:41042597-41042616 MS.gene63185:intron 20.0%
!!! ATTTGATTTGCTAAAATACA+GGG - chr8.4:41041950-41041969 None:intergenic 20.0%
! AAAAAAAAACAAGGTAAGAC+TGG - chr8.4:41041791-41041810 None:intergenic 25.0%
! AAAAAAAACAAGGTAAGACT+GGG - chr8.4:41041790-41041809 None:intergenic 25.0%
! AAAAACTAAAAGGACTAATC+TGG + chr8.4:41041510-41041529 MS.gene63185:intron 25.0%
! AAGATTAACTGATATACCAA+GGG + chr8.4:41043168-41043187 MS.gene63185:CDS 25.0%
! AGAGAGATATATACATACAT+AGG - chr8.4:41042878-41042897 None:intergenic 25.0%
! AGTAACTAACAAAAATGAGT+AGG - chr8.4:41041192-41041211 None:intergenic 25.0%
! ATATCCTTCTATGTGAATAA+AGG - chr8.4:41043026-41043045 None:intergenic 25.0%
! CAGTTTCTAAATCAACAAAA+GGG + chr8.4:41041695-41041714 MS.gene63185:intron 25.0%
! GATGTATATCACCATTATTT+CGG - chr8.4:41042554-41042573 None:intergenic 25.0%
! TCAGTTTCTAAATCAACAAA+AGG + chr8.4:41041694-41041713 MS.gene63185:intron 25.0%
! TGATGTAAATTGCAAAATTC+TGG - chr8.4:41042723-41042742 None:intergenic 25.0%
!! AATTCTGGATAATTTAGAAC+AGG - chr8.4:41042708-41042727 None:intergenic 25.0%
!! ATTCTGGATAATTTAGAACA+GGG - chr8.4:41042707-41042726 None:intergenic 25.0%
!! GTTCAATTTTACTGACATTA+AGG + chr8.4:41041471-41041490 MS.gene63185:intron 25.0%
!!! ATTTCGCTTAGTTTTTATGT+TGG + chr8.4:41041565-41041584 MS.gene63185:intron 25.0%
!!! CATTTGATTTGCTAAAATAC+AGG - chr8.4:41041951-41041970 None:intergenic 25.0%
!!! TAAGCACTTTTTAACAAAGT+TGG + chr8.4:41041400-41041419 MS.gene63185:intron 25.0%
AATTTCAACCTCTTGAAGAA+AGG - chr8.4:41042345-41042364 None:intergenic 30.0%
AGAATCTTGCCAAATTCTTA+AGG - chr8.4:41041976-41041995 None:intergenic 30.0%
AGATTAACTGATATACCAAG+GGG + chr8.4:41043169-41043188 MS.gene63185:CDS 30.0%
AGTTTCAACATCTTCAATGA+TGG - chr8.4:41042165-41042184 None:intergenic 30.0%
ATCTTGATCACAGTATTCAA+TGG - chr8.4:41041108-41041127 None:intergenic 30.0%
ATTCACATAGAAGGATATCA+AGG + chr8.4:41043028-41043047 MS.gene63185:CDS 30.0%
CAGCTGAATCAAATTTAAGT+TGG - chr8.4:41043207-41043226 None:intergenic 30.0%
GAAGATTAACTGATATACCA+AGG + chr8.4:41043167-41043186 MS.gene63185:CDS 30.0%
TAAAATCCTAGGATGCATTT+GGG + chr8.4:41041542-41041561 MS.gene63185:intron 30.0%
TAAAGTTGTTGTCATATGAC+TGG - chr8.4:41041858-41041877 None:intergenic 30.0%
TAGAACAGTTCTTGTTAGTT+AGG - chr8.4:41043144-41043163 None:intergenic 30.0%
TCAAATGCACCTTAAGAATT+TGG + chr8.4:41041964-41041983 MS.gene63185:intron 30.0%
TCATTGAAGATGTTGAAACT+CGG + chr8.4:41042165-41042184 MS.gene63185:CDS 30.0%
TTAAAATCCTAGGATGCATT+TGG + chr8.4:41041541-41041560 MS.gene63185:intron 30.0%
TTCAAGAGGTTGAAATTAAC+CGG + chr8.4:41042348-41042367 MS.gene63185:CDS 30.0%
TTGAATACTGTGATCAAGAT+TGG + chr8.4:41041108-41041127 MS.gene63185:CDS 30.0%
! ATTTTTGTTAGTTACTGCCT+CGG + chr8.4:41041196-41041215 MS.gene63185:intron 30.0%
! GTTGATTTAGAAACTGATCA+GGG - chr8.4:41041691-41041710 None:intergenic 30.0%
! TCATGAAAGCAACCATATTT+TGG - chr8.4:41042015-41042034 None:intergenic 30.0%
! TTCTGGATAATTTAGAACAG+GGG - chr8.4:41042706-41042725 None:intergenic 30.0%
!! CATTTGCTGAATATGTTTTG+CGG + chr8.4:41043068-41043087 MS.gene63185:CDS 30.0%
!! TGTTGATTTAGAAACTGATC+AGG - chr8.4:41041692-41041711 None:intergenic 30.0%
!!! CAAGAATTCTTGTTTTTGTC+CGG + chr8.4:41041664-41041683 MS.gene63185:intron 30.0%
!!! CGTTTTTTTCGATACATCAA+TGG + chr8.4:41043117-41043136 MS.gene63185:CDS 30.0%
AAAATGAGTAGGCTTACCTA+AGG - chr8.4:41041181-41041200 None:intergenic 35.0%
AAATGAGTAGGCTTACCTAA+GGG - chr8.4:41041180-41041199 None:intergenic 35.0%
AAGAATTTGGCAAGATTCTC+AGG + chr8.4:41041977-41041996 MS.gene63185:intron 35.0%
ATTCGACAACATAGACATTG+TGG + chr8.4:41042227-41042246 MS.gene63185:CDS 35.0%
CTGACCTTTATTCACATAGA+AGG + chr8.4:41043019-41043038 MS.gene63185:CDS 35.0%
CTTCTATGTGAATAAAGGTC+AGG - chr8.4:41043021-41043040 None:intergenic 35.0%
GATGGAAGATGAATTGAAGA+AGG - chr8.4:41042147-41042166 None:intergenic 35.0%
GGTACAAACGTTAAAATCCT+AGG + chr8.4:41041531-41041550 MS.gene63185:intron 35.0%
GTATATCACCATTATTTCGG+AGG - chr8.4:41042551-41042570 None:intergenic 35.0%
GTCTTCAAATAGATTCTGAC+TGG + chr8.4:41042391-41042410 MS.gene63185:CDS 35.0%
TATGCGTCATTTCACATCAA+AGG + chr8.4:41042578-41042597 MS.gene63185:CDS 35.0%
TATGTGAATAAAGGTCAGGT+GGG - chr8.4:41043017-41043036 None:intergenic 35.0%
TCATTACTTGTCTTTCTCTC+TGG + chr8.4:41042252-41042271 MS.gene63185:CDS 35.0%
TCTCTTAATTCAGAGTAGCA+AGG + chr8.4:41042818-41042837 MS.gene63185:CDS 35.0%
TTAAGAGAACTTGAAGCACA+TGG - chr8.4:41042806-41042825 None:intergenic 35.0%
TTGGTCAAAACATTGTCACA+TGG + chr8.4:41041629-41041648 MS.gene63185:intron 35.0%
TTTGCTAAAATACAGGGAAC+TGG - chr8.4:41041944-41041963 None:intergenic 35.0%
! AACCATCACCTTTAAGAACA+TGG + chr8.4:41042954-41042973 MS.gene63185:CDS 35.0%
! ACCATCACCTTTAAGAACAT+GGG + chr8.4:41042955-41042974 MS.gene63185:CDS 35.0%
! ACGAAACTTTTTAATGTCGC+GGG - chr8.4:41040985-41041004 None:intergenic 35.0%
! GACGAAACTTTTTAATGTCG+CGG - chr8.4:41040986-41041005 None:intergenic 35.0%
! TGAAAAAACTGCAGTCTTGT+TGG + chr8.4:41042099-41042118 MS.gene63185:intron 35.0%
!! ACAAACAGTTCTTCAAGTTG+AGG - chr8.4:41041083-41041102 None:intergenic 35.0%
!! CCTTCTAGCTCAACTTTAAA+CGG + chr8.4:41041372-41041391 MS.gene63185:intron 35.0%
!! GTAGGAATTGTTGTTGACTT+TGG - chr8.4:41042983-41043002 None:intergenic 35.0%
!!! TTGGTGTCATAAGCAGATTT+TGG + chr8.4:41041348-41041367 MS.gene63185:intron 35.0%
AAAAAGTTTCGTCTCACCAG+TGG + chr8.4:41040993-41041012 MS.gene63185:CDS 40.0%
AAAACCACATTGGGCATCAT+TGG + chr8.4:41042444-41042463 MS.gene63185:CDS 40.0%
AAATCAACAAAAGGGCAACC+CGG + chr8.4:41041703-41041722 MS.gene63185:intron 40.0%
ACTGGGTACAATACACCAAA+TGG - chr8.4:41041773-41041792 None:intergenic 40.0%
AGAAGACTCATGTTGAAGCA+GGG - chr8.4:41042762-41042781 None:intergenic 40.0%
AGAGTATATGGAAGAGGAGT+GGG - chr8.4:41040782-41040801 None:intergenic 40.0%
AGTCCAAGAGAAATTTACGC+CGG - chr8.4:41042370-41042389 None:intergenic 40.0%
CACAAATTCACTTTCACGGA+GGG - chr8.4:41042511-41042530 None:intergenic 40.0%
CCTCAGTTTCTGAATCAACA+GGG + chr8.4:41041898-41041917 MS.gene63185:intron 40.0%
CTATGTGAATAAAGGTCAGG+TGG - chr8.4:41043018-41043037 None:intergenic 40.0%
GCAAACCTTCTGAACAATCT+TGG - chr8.4:41040927-41040946 None:intergenic 40.0%
GGTACAATACACCAAATGGT+GGG - chr8.4:41041769-41041788 None:intergenic 40.0%
GGTCCACAAATTCACTTTCA+CGG - chr8.4:41042515-41042534 None:intergenic 40.0%
GTACGCATAATCACAAGTGA+TGG - chr8.4:41042664-41042683 None:intergenic 40.0%
GTGCAATCAAGAAGAGAAGA+AGG - chr8.4:41041146-41041165 None:intergenic 40.0%
GTTGTTGTCATATGACTGGA+AGG - chr8.4:41041854-41041873 None:intergenic 40.0%
TAACCGGCGTAAATTTCTCT+TGG + chr8.4:41042364-41042383 MS.gene63185:CDS 40.0%
TCAAGGATTTGAAGATGAGC+TGG + chr8.4:41043045-41043064 MS.gene63185:CDS 40.0%
TCCCATGTTCTTAAAGGTGA+TGG - chr8.4:41042959-41042978 None:intergenic 40.0%
TCTGTTTCAAACTAAGACCG+AGG - chr8.4:41041216-41041235 None:intergenic 40.0%
TTACCTAAGGGAAACGAGAT+GGG - chr8.4:41041168-41041187 None:intergenic 40.0%
TTTAGAAACTGATCAGGGAC+CGG - chr8.4:41041686-41041705 None:intergenic 40.0%
! AATCTAGAGTTTCGAGTACG+GGG - chr8.4:41042281-41042300 None:intergenic 40.0%
! AGGTGGGATTTGAAACATGT+AGG - chr8.4:41043001-41043020 None:intergenic 40.0%
! CAATCTAGAGTTTCGAGTAC+GGG - chr8.4:41042282-41042301 None:intergenic 40.0%
! CCGTTTAAAGTTGAGCTAGA+AGG - chr8.4:41041375-41041394 None:intergenic 40.0%
! GCAATCTAGAGTTTCGAGTA+CGG - chr8.4:41042283-41042302 None:intergenic 40.0%
! GCTGAATATGTTTTGCGGAA+CGG + chr8.4:41043073-41043092 MS.gene63185:CDS 40.0%
! TGGTAGAGTGCTTAATCTGT+CGG - chr8.4:41040757-41040776 None:intergenic 40.0%
! TTTGGCTCCCATGTTCTTAA+AGG - chr8.4:41042965-41042984 None:intergenic 40.0%
!! CCTGTTGATTCAGAAACTGA+GGG - chr8.4:41041900-41041919 None:intergenic 40.0%
!! CGTCTTTTGTGCTAGTTTCT+TGG + chr8.4:41042302-41042321 MS.gene63185:CDS 40.0%
!! CTGTTGATTCAGAAACTGAG+GGG - chr8.4:41041899-41041918 None:intergenic 40.0%
AACGGCCAAGATTGTTCAGA+AGG + chr8.4:41040919-41040938 MS.gene63185:CDS 45.0%
ACTCACACGAAGACGGAAAA+CGG + chr8.4:41040901-41040920 MS.gene63185:CDS 45.0%
ATTCGACATTGGCAACACTG+TGG + chr8.4:41042203-41042222 MS.gene63185:CDS 45.0%
CAGAAGACTCATGTTGAAGC+AGG - chr8.4:41042763-41042782 None:intergenic 45.0%
CATTGGAAACTTGCAGAGTG+TGG + chr8.4:41042461-41042480 MS.gene63185:CDS 45.0%
CCACAAATTCACTTTCACGG+AGG - chr8.4:41042512-41042531 None:intergenic 45.0%
CCCTCAGTTTCTGAATCAAC+AGG + chr8.4:41041897-41041916 MS.gene63185:intron 45.0%
CCTCCGTGAAAGTGAATTTG+TGG + chr8.4:41042509-41042528 MS.gene63185:CDS 45.0%
CCTCTTGAAGAAAGGTGACA+CGG - chr8.4:41042337-41042356 None:intergenic 45.0%
CGAAATCCCAAATGCATCCT+AGG - chr8.4:41041551-41041570 None:intergenic 45.0%
CTCAGCTACCTCCGAAATAA+TGG + chr8.4:41042540-41042559 MS.gene63185:CDS 45.0%
CTCTTGAAGAAAGGTGACAC+GGG - chr8.4:41042336-41042355 None:intergenic 45.0%
CTTACCTAAGGGAAACGAGA+TGG - chr8.4:41041169-41041188 None:intergenic 45.0%
GGGTACAATACACCAAATGG+TGG - chr8.4:41041770-41041789 None:intergenic 45.0%
GTCATATGACTGGAAGGTCA+CGG - chr8.4:41041848-41041867 None:intergenic 45.0%
GTCATCATTCAACAGACCAC+TGG - chr8.4:41041012-41041031 None:intergenic 45.0%
GTGATTATGCGTACAAGCAG+TGG + chr8.4:41042670-41042689 MS.gene63185:intron 45.0%
GTGGTCTGTTGAATGATGAC+TGG + chr8.4:41041012-41041031 MS.gene63185:CDS 45.0%
GTTTCCAATGATGCCCAATG+TGG - chr8.4:41042451-41042470 None:intergenic 45.0%
GTTTGCATTCTTCGTCAACG+CGG + chr8.4:41040941-41040960 MS.gene63185:CDS 45.0%
TAGCGATTGCTTCGAGGTAT+GGG + chr8.4:41041040-41041059 MS.gene63185:CDS 45.0%
TCATATGACTGGAAGGTCAC+GGG - chr8.4:41041847-41041866 None:intergenic 45.0%
! GAGAGTATATGGAAGAGGAG+TGG - chr8.4:41040783-41040802 None:intergenic 45.0%
! GGAAGGAGAGTATATGGAAG+AGG - chr8.4:41040788-41040807 None:intergenic 45.0%
! TAGAGTGCTTAATCTGTCGG+TGG - chr8.4:41040754-41040773 None:intergenic 45.0%
! TTACACAAGAGGCTGTTTCC+AGG + chr8.4:41041814-41041833 MS.gene63185:intron 45.0%
! TTGGGAGGAAGGAGAGTATA+TGG - chr8.4:41040794-41040813 None:intergenic 45.0%
! TTTTAATGTCGCGGGATCGA+CGG - chr8.4:41040977-41040996 None:intergenic 45.0%
!! CCCTGTTGATTCAGAAACTG+AGG - chr8.4:41041901-41041920 None:intergenic 45.0%
!! TTCTTCAAGTTGAGGCCCAA+TGG - chr8.4:41041075-41041094 None:intergenic 45.0%
AAGATGTTGAAACTCGGCGC+AGG + chr8.4:41042171-41042190 MS.gene63185:CDS 50.0%
ACTAAAGCTCCCGCATACAC+AGG + chr8.4:41041729-41041748 MS.gene63185:intron 50.0%
AGCACGGGCAAAACCACATT+GGG + chr8.4:41042435-41042454 MS.gene63185:CDS 50.0%
ATATGGAAGAGGAGTGGGTC+TGG - chr8.4:41040777-41040796 None:intergenic 50.0%
CAACTTTACCAGTTGCGCCA+AGG + chr8.4:41041870-41041889 MS.gene63185:intron 50.0%
CAGAAACTGAGGGGTAACCT+TGG - chr8.4:41041890-41041909 None:intergenic 50.0%
CCGTGTCACCTTTCTTCAAG+AGG + chr8.4:41042334-41042353 MS.gene63185:CDS 50.0%
CTAAAGCTCCCGCATACACA+GGG + chr8.4:41041730-41041749 MS.gene63185:intron 50.0%
GAAAGGTGACACGGGAACAT+TGG - chr8.4:41042328-41042347 None:intergenic 50.0%
GGCGTTGTGGAATTCGACAT+TGG + chr8.4:41042192-41042211 MS.gene63185:CDS 50.0%
GTAGCGATTGCTTCGAGGTA+TGG + chr8.4:41041039-41041058 MS.gene63185:CDS 50.0%
GTGCTTAATCTGTCGGTGGT+CGG - chr8.4:41040750-41040769 None:intergenic 50.0%
GTTCCTCCTAGCAGCAACAA+GGG - chr8.4:41042937-41042956 None:intergenic 50.0%
GTTTCGTAGCGATTGCTTCG+AGG + chr8.4:41041034-41041053 MS.gene63185:CDS 50.0%
TAGCACGGGCAAAACCACAT+TGG + chr8.4:41042434-41042453 MS.gene63185:CDS 50.0%
TGAGTCGTCGGAGAATTCGA+AGG - chr8.4:41040886-41040905 None:intergenic 50.0%
TGGAAACTTGCAGAGTGTGG+AGG + chr8.4:41042464-41042483 MS.gene63185:CDS 50.0%
TGTAGACGACAGTCGTAGCA+CGG + chr8.4:41042419-41042438 MS.gene63185:CDS 50.0%
TTGTGTCCCTTGTTGCTGCT+AGG + chr8.4:41042928-41042947 MS.gene63185:intron 50.0%
! TTTAGAACAGGGGTGCGAAG+TGG - chr8.4:41042696-41042715 None:intergenic 50.0%
AAGGTCACGGGTTCAAGTCC+TGG - chr8.4:41041835-41041854 None:intergenic 55.0%
CTTAATCTGTCGGTGGTCGG+CGG - chr8.4:41040747-41040766 None:intergenic 55.0%
GCACCCATCTCGTTTCCCTT+AGG + chr8.4:41041162-41041181 MS.gene63185:CDS 55.0%
GGTTCCTCCTAGCAGCAACA+AGG - chr8.4:41042938-41042957 None:intergenic 55.0%
GTAGACGACAGTCGTAGCAC+GGG + chr8.4:41042420-41042439 MS.gene63185:CDS 55.0%
TATGGGTCCGTGCTGCCATT+GGG + chr8.4:41041057-41041076 MS.gene63185:CDS 55.0%
TCCGACGACTCACACGAAGA+CGG + chr8.4:41040894-41040913 MS.gene63185:CDS 55.0%
TCCGTCTTCGTGTGAGTCGT+CGG - chr8.4:41040898-41040917 None:intergenic 55.0%
TGTCCCTTGTTGCTGCTAGG+AGG + chr8.4:41042931-41042950 MS.gene63185:intron 55.0%
TTCGGAGGTAGCTGAGCACA+GGG - chr8.4:41042536-41042555 None:intergenic 55.0%
TTTCGGAGGTAGCTGAGCAC+AGG - chr8.4:41042537-41042556 None:intergenic 55.0%
! ATGCGGGAGCTTTAGTGCAC+CGG - chr8.4:41041725-41041744 None:intergenic 55.0%
AATGTCGCGGGATCGACGGA+GGG - chr8.4:41040973-41040992 None:intergenic 60.0%
AGGGGTAACCTTGGCGCAAC+TGG - chr8.4:41041881-41041900 None:intergenic 60.0%
CATACACAGGGTCCGGGAAG+GGG + chr8.4:41041742-41041761 MS.gene63185:intron 60.0%
CTCGCAGGTGGCGAAATCTC+TGG + chr8.4:41040847-41040866 MS.gene63185:CDS 60.0%
CTTCCCGGACCCTGTGTATG+CGG - chr8.4:41041742-41041761 None:intergenic 60.0%
GCATACACAGGGTCCGGGAA+GGG + chr8.4:41041741-41041760 MS.gene63185:intron 60.0%
GTATGGGTCCGTGCTGCCAT+TGG + chr8.4:41041056-41041075 MS.gene63185:CDS 60.0%
GTTGAGGCCCAATGGCAGCA+CGG - chr8.4:41041067-41041086 None:intergenic 60.0%
TAATGTCGCGGGATCGACGG+AGG - chr8.4:41040974-41040993 None:intergenic 60.0%
TGGCATGTGACAGAGCCCCT+TGG - chr8.4:41043187-41043206 None:intergenic 60.0%
TTCCCGGACCCTGTGTATGC+GGG - chr8.4:41041741-41041760 None:intergenic 60.0%
TTCGCCACCTGCGAGAGATG+AGG - chr8.4:41040842-41040861 None:intergenic 60.0%
! CAAATGGTGGGACCCCTTCC+CGG - chr8.4:41041757-41041776 None:intergenic 60.0%
! GGAGGTTCTGGTTGGGAGGA+AGG - chr8.4:41040805-41040824 None:intergenic 60.0%
! TCGCCACGGAGGTTCTGGTT+GGG - chr8.4:41040812-41040831 None:intergenic 60.0%
! TGCGGGAGCTTTAGTGCACC+GGG - chr8.4:41041724-41041743 None:intergenic 60.0%
CCTCCCAACCAGAACCTCCG+TGG + chr8.4:41040806-41040825 MS.gene63185:CDS 65.0%
CGACGAGCCTCATCTCTCGC+AGG + chr8.4:41040832-41040851 MS.gene63185:CDS 65.0%
CGAGCCTCATCTCTCGCAGG+TGG + chr8.4:41040835-41040854 MS.gene63185:CDS 65.0%
CGCATACACAGGGTCCGGGA+AGG + chr8.4:41041740-41041759 MS.gene63185:intron 65.0%
CGGGAAGGGGTCCCACCATT+TGG + chr8.4:41041755-41041774 MS.gene63185:intron 65.0%
CTCCCGCATACACAGGGTCC+GGG + chr8.4:41041736-41041755 MS.gene63185:intron 65.0%
GATGAGGCTCGTCGTCGCCA+CGG - chr8.4:41040826-41040845 None:intergenic 65.0%
GCTCCCGCATACACAGGGTC+CGG + chr8.4:41041735-41041754 MS.gene63185:intron 65.0%
! CCACGGAGGTTCTGGTTGGG+AGG - chr8.4:41040809-41040828 None:intergenic 65.0%
! GAAACTCGGCGCAGGCGTTG+TGG + chr8.4:41042179-41042198 MS.gene63185:CDS 65.0%
! GTCGCCACGGAGGTTCTGGT+TGG - chr8.4:41040813-41040832 None:intergenic 65.0%
CGTCGTCGCCACGGAGGTTC+TGG - chr8.4:41040817-41040836 None:intergenic 70.0%
GAGGCTCGTCGTCGCCACGG+AGG - chr8.4:41040823-41040842 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 41040738 41043237 41040738 ID=MS.gene63185
chr8.4 mRNA 41040738 41043237 41040738 ID=MS.gene63185.t1;Parent=MS.gene63185
chr8.4 exon 41040738 41041183 41040738 ID=MS.gene63185.t1.exon1;Parent=MS.gene63185.t1
chr8.4 CDS 41040738 41041183 41040738 ID=cds.MS.gene63185.t1;Parent=MS.gene63185.t1
chr8.4 exon 41042113 41042599 41042113 ID=MS.gene63185.t1.exon2;Parent=MS.gene63185.t1
chr8.4 CDS 41042113 41042599 41042113 ID=cds.MS.gene63185.t1;Parent=MS.gene63185.t1
chr8.4 exon 41042690 41042839 41042690 ID=MS.gene63185.t1.exon3;Parent=MS.gene63185.t1
chr8.4 CDS 41042690 41042839 41042690 ID=cds.MS.gene63185.t1;Parent=MS.gene63185.t1
chr8.4 exon 41042950 41043237 41042950 ID=MS.gene63185.t1.exon4;Parent=MS.gene63185.t1
chr8.4 CDS 41042950 41043237 41042950 ID=cds.MS.gene63185.t1;Parent=MS.gene63185.t1
Gene Sequence

>MS.gene63185

ATGGCGCCGCCGACCACCGACAGATTAAGCACTCTACCAGACCCACTCCTCTTCCATATACTCTCCTTCCTCCCAACCAGAACCTCCGTGGCGACGACGAGCCTCATCTCTCGCAGGTGGCGAAATCTCTGGCAGAATCTACAAGCCTTCGAATTCTCCGACGACTCACACGAAGACGGAAAACGGCCAAGATTGTTCAGAAGGTTTGCATTCTTCGTCAACGCGGTGCTCTCCCTCCGTCGATCCCGCGACATTAAAAAGTTTCGTCTCACCAGTGGTCTGTTGAATGATGACTGGTTTCGTAGCGATTGCTTCGAGGTATGGGTCCGTGCTGCCATTGGGCCTCAACTTGAAGAACTGTTTGTTTCCATTGAATACTGTGATCAAGATTGGCAAGTGCTCGTTCCTTCTTCTCTTCTTGATTGCACCCATCTCGTTTCCCTTAGGTAAGCCTACTCATTTTTGTTAGTTACTGCCTCGGTCTTAGTTTGAAACAGAATCAATTACTTGATTTTATAATGTTGTTGCTGAAATGTTTATCTGTAAAAATTAGGATTGAATTGAGAACCTATAATACCAATAATAAGGAAATGTATGTAAGTAATCATATTTGGTGTCATAAGCAGATTTTGGACCTTCTAGCTCAACTTTAAACGGTCATGTAAGCACTTTTTAACAAAGTTGGATGAAAAAAATGATCAATATGTTACAAAAAAGAAAATTAGAGGTCTAAGTTCAATTTTACTGACATTAAGGACCAATTTGAAACTAAAAAAACTAAAAGGACTAATCTGGTACAAACGTTAAAATCCTAGGATGCATTTGGGATTTCGCTTAGTTTTTATGTTGGCAAATAAAAATGGTATTTTGCTATTTTTGAAAGTATGTAATTTGGTCAAAACATTGTCACATGGTTTAGCGAGTGACAAGAATTCTTGTTTTTGTCCGGTCCCTGATCAGTTTCTAAATCAACAAAAGGGCAACCCGGTGCACTAAAGCTCCCGCATACACAGGGTCCGGGAAGGGGTCCCACCATTTGGTGTATTGTACCCAGTCTTACCTTGTTTTTTTTTTTTTTACACAAGAGGCTGTTTCCAGGACTTGAACCCGTGACCTTCCAGTCATATGACAACAACTTTACCAGTTGCGCCAAGGTTACCCCTCAGTTTCTGAATCAACAGGGTACAAAAAAAGTTGTACAGTCCAGTTCCCTGTATTTTAGCAAATCAAATGCACCTTAAGAATTTGGCAAGATTCTCAGGTTTTAATAATATCCAAAATATGGTTGCTTTCATGATTTCATCTCTTGTTCTCTCTCTGTTTTTAACATGTATATATAGAATGTTATGTAACATGTGTTTTGAAAAAACTGCAGTCTTGTTGGTGCAATTCATATAATATTTCAACCTTCTTCAATTCATCTTCCATCATTGAAGATGTTGAAACTCGGCGCAGGCGTTGTGGAATTCGACATTGGCAACACTGTGGAATTCGACAACATAGACATTGTGGATTCATTACTTGTCTTTCTCTCTGGCTGCCCCGTACTCGAAACTCTAGATTGCGTCTTTTGTGCTAGTTTCTTGGCCAATGTTCCCGTGTCACCTTTCTTCAAGAGGTTGAAATTAACCGGCGTAAATTTCTCTTGGACTTGTCTTCAAATAGATTCTGACTGGCTCTGTGTAGACGACAGTCGTAGCACGGGCAAAACCACATTGGGCATCATTGGAAACTTGCAGAGTGTGGAGGAAGCATATTTTGATGTCTTTTCCCTCCGTGAAAGTGAATTTGTGGACCCTGTGCTCAGCTACCTCCGAAATAATGGTGATATACATCTACTTATGCGTCATTTCACATCAAAGGTGAAGTTTTATTATTATGGTTTATATGTATTTATATATGTACTTCTGTACTAATGTTGTGCCATCACTTGTGATTATGCGTACAAGCAGTGGCCACTTCGCACCCCTGTTCTAAATTATCCAGAATTTTGCAATTTACATCATCTAAAGTTCATTCTTCCCTGCTTCAACATGAGTCTTCTGCTCAACGTGCTTGAGAAATGCCATGTGCTTCAAGTTCTCTTAATTCAGAGTAGCAAGGTTTGCTTTATTTAAATTAATTTTTGGTCTATCTACCTATGTATGTATATATCTCTCTCTATATATACACACAAATAGAATTGAGTCATTGTGTCCCTTGTTGCTGCTAGGAGGAACCATCACCTTTAAGAACATGGGAGCCAAAGTCAACAACAATTCCTACATGTTTCAAATCCCACCTGACCTTTATTCACATAGAAGGATATCAAGGATTTGAAGATGAGCTGGCATTTGCTGAATATGTTTTGCGGAACGGACTTGTTTTACACACAATGCTCGTTTTTTTCGATACATCAATGGACCTAACTAACAAGAACTGTTCTATCAGAAGATTAACTGATATACCAAGGGGCTCTGTCACATGCCAACTTAAATTTGATTCAGCTGTGTCTCCATAA
Protein sequence

>MS.gene63185.t1

MAPPTTDRLSTLPDPLLFHILSFLPTRTSVATTSLISRRWRNLWQNLQAFEFSDDSHEDGKRPRLFRRFAFFVNAVLSLRRSRDIKKFRLTSGLLNDDWFRSDCFEVWVRAAIGPQLEELFVSIEYCDQDWQVLVPSSLLDCTHLVSLSLVGAIHIIFQPSSIHLPSLKMLKLGAGVVEFDIGNTVEFDNIDIVDSLLVFLSGCPVLETLDCVFCASFLANVPVSPFFKRLKLTGVNFSWTCLQIDSDWLCVDDSRSTGKTTLGIIGNLQSVEEAYFDVFSLRESEFVDPVLSYLRNNGDIHLLMRHFTSKWPLRTPVLNYPEFCNLHHLKFILPCFNMSLLLNVLEKCHVLQVLLIQSSKEEPSPLRTWEPKSTTIPTCFKSHLTFIHIEGYQGFEDELAFAEYVLRNGLVLHTMLVFFDTSMDLTNKNCSIRRLTDIPRGSVTCQLKFDSAVSP