AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72955

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72955.t1 MTR_8g468810 80.215 465 41 6 1 416 1 463 0.0 724
MS.gene72955.t1 MTR_8g468810 78.016 373 31 6 1 324 1 371 0.0 553
MS.gene72955.t1 MTR_8g058970 55.579 484 140 8 1 416 1 477 4.34e-158 454
MS.gene72955.t1 MTR_8g468810 75.157 318 28 6 1 269 1 316 7.11e-156 443
MS.gene72955.t1 MTR_8g468810 75.394 317 27 6 1 268 1 315 1.37e-155 442
MS.gene72955.t1 MTR_8g468810 75.394 317 27 6 1 268 1 315 1.44e-155 443
MS.gene72955.t1 MTR_2g083840 47.845 464 173 11 6 416 10 457 4.27e-123 365
MS.gene72955.t1 MTR_8g062270 44.273 454 174 14 25 415 16 453 2.88e-102 311
MS.gene72955.t1 MTR_5g092960 43.856 472 185 14 10 415 4 461 1.04e-100 307
MS.gene72955.t1 MTR_8g468840 44.543 449 176 15 25 414 14 448 6.28e-99 302
MS.gene72955.t1 MTR_4g032730 41.201 483 188 12 11 415 2 466 2.60e-98 301
MS.gene72955.t1 MTR_8g468760 42.672 464 159 12 12 415 2 418 4.79e-92 284
MS.gene72955.t1 MTR_8g014260 39.076 476 205 18 1 416 1 451 1.77e-75 242
MS.gene72955.t1 MTR_8g070950 36.059 477 201 14 1 416 1 434 2.46e-72 233
MS.gene72955.t1 MTR_8g467800 49.237 262 112 6 164 416 204 453 1.82e-71 232
MS.gene72955.t1 MTR_8g467800 48.571 140 58 5 5 139 3 133 4.28e-28 115
MS.gene72955.t1 MTR_5g092960 42.175 377 147 12 10 324 4 371 3.65e-69 224
MS.gene72955.t1 MTR_8g469020 36.226 461 211 16 24 416 6 451 3.45e-66 218
MS.gene72955.t1 MTR_8g058965 36.966 468 220 12 11 416 2 456 1.72e-65 216
MS.gene72955.t1 MTR_8g062310 36.184 456 215 14 4 416 6 428 4.01e-64 212
MS.gene72955.t1 MTR_8g062080 35.512 459 229 15 5 416 7 445 9.07e-63 209
MS.gene72955.t1 MTR_8g445780 39.480 423 165 17 1 366 1 389 8.47e-62 205
MS.gene72955.t1 MTR_8g467800 45.130 308 108 12 5 260 3 301 1.96e-59 197
MS.gene72955.t1 MTR_8g077460 34.217 415 217 14 17 416 2 375 1.91e-54 185
MS.gene72955.t1 MTR_4g050250 36.028 433 179 15 4 358 6 418 3.48e-50 175
MS.gene72955.t1 MTR_8g014210 45.679 243 119 9 175 416 1 231 3.30e-47 162
MS.gene72955.t1 MTR_4g024710 40.753 292 148 12 130 416 76 347 2.82e-45 160
MS.gene72955.t1 MTR_8g467790 31.653 357 206 10 25 356 148 491 1.79e-36 140
MS.gene72955.t1 MTR_8g014160 30.698 430 233 15 16 416 32 425 8.00e-35 134
MS.gene72955.t1 MTR_4g024745 39.700 267 141 10 130 392 30 280 1.00e-34 130
MS.gene72955.t1 MTR_5g021550 50.331 151 58 5 1 143 1 142 1.58e-34 126
MS.gene72955.t1 MTR_8g062010 40.816 196 106 4 222 416 244 430 5.91e-34 131
MS.gene72955.t1 MTR_8g062010 50.794 126 44 4 25 139 9 127 3.68e-28 115
MS.gene72955.t1 MTR_8g062300 32.948 346 189 11 90 416 156 477 3.80e-32 127
MS.gene72955.t1 MTR_8g062300 44.371 151 65 5 1 139 1 144 2.08e-25 108
MS.gene72955.t1 MTR_8g014290 42.657 143 69 5 17 147 2 143 4.10e-26 103
MS.gene72955.t1 MTR_7g032380 26.923 416 226 13 17 416 3 356 1.99e-23 100
MS.gene72955.t1 MTR_7g098510 25.899 417 249 13 21 416 14 391 8.07e-23 99.8
MS.gene72955.t1 MTR_3g036250 27.251 411 227 15 24 413 4 363 1.57e-22 98.6
MS.gene72955.t1 MTR_4g024720 42.029 138 61 6 1 130 1 127 2.58e-22 94.7
MS.gene72955.t1 MTR_7g098570 27.423 423 249 13 10 412 5 389 6.41e-22 97.8
MS.gene72955.t1 MTR_5g058000 28.369 423 225 17 20 415 3 374 8.92e-21 93.6
MS.gene72955.t1 MTR_5g080710 27.045 440 243 19 23 408 31 446 2.33e-20 93.2
MS.gene72955.t1 MTR_7g033915 28.641 412 219 17 23 415 9 364 4.08e-20 91.7
MS.gene72955.t1 MTR_5g058100 26.824 425 241 15 18 415 2 383 6.04e-19 88.2
MS.gene72955.t1 MTR_3g023630 25.553 407 240 16 22 409 5 367 8.24e-19 87.8
MS.gene72955.t1 MTR_5g073420 24.764 424 231 16 25 415 11 379 1.25e-18 87.4
MS.gene72955.t1 MTR_2g086020 42.202 109 61 1 308 416 209 315 3.35e-18 85.5
MS.gene72955.t1 MTR_2g086020 44.898 98 38 3 51 139 1 91 2.53e-16 79.7
MS.gene72955.t1 MTR_7g026500 24.582 419 250 12 25 415 14 394 1.07e-17 84.7
MS.gene72955.t1 MTR_5g008920 25.899 417 246 17 22 416 6 381 1.44e-17 84.3
MS.gene72955.t1 MTR_2g008320 27.229 415 240 20 25 416 52 427 1.46e-17 84.7
MS.gene72955.t1 MTR_7g056433 26.478 423 235 13 25 416 12 389 1.86e-17 84.0
MS.gene72955.t1 MTR_3g108150 27.945 433 225 20 18 415 6 386 1.95e-17 84.0
MS.gene72955.t1 MTR_2g008310 26.355 406 249 18 25 416 2 371 2.37e-17 83.6
MS.gene72955.t1 MTR_6g011460 24.658 438 258 15 7 416 28 421 5.79e-17 82.8
MS.gene72955.t1 MTR_0543s0010 25.721 416 232 13 25 415 86 449 3.39e-16 80.5
MS.gene72955.t1 MTR_4g024770 56.164 73 21 3 1 72 8 70 4.89e-16 73.2
MS.gene72955.t1 MTR_6g044560 24.831 443 271 18 2 416 156 564 1.04e-15 79.3
MS.gene72955.t1 MTR_1089s0010 25.537 419 238 18 25 416 8 379 1.20e-15 78.6
MS.gene72955.t1 MTR_6g022360 26.425 386 212 16 21 382 39 376 2.24e-15 77.8
MS.gene72955.t1 MTR_2g007400 24.594 431 257 17 20 416 7 403 3.14e-15 77.4
MS.gene72955.t1 MTR_6g044572 24.524 420 256 17 25 416 2 388 9.00e-15 75.9
MS.gene72955.t1 MTR_5g084070 25.490 408 223 17 23 377 11 390 1.04e-14 75.9
MS.gene72955.t1 MTR_3g107120 26.988 415 246 18 23 413 7 388 1.24e-14 75.5
MS.gene72955.t1 MTR_1g108820 26.128 421 236 16 24 413 7 383 2.12e-14 74.7
MS.gene72955.t1 MTR_6g040270 26.619 417 228 19 27 413 10 378 2.57e-14 74.3
MS.gene72955.t1 MTR_8g014230 50.685 73 32 2 334 406 27 95 1.16e-12 64.3
MS.gene72955.t1 MTR_2g005990 26.107 429 237 20 24 413 5 392 2.30e-12 68.6
MS.gene72955.t1 MTR_7g034110 25.070 355 189 13 23 367 9 296 3.80e-12 67.4
MS.gene72955.t1 MTR_2g007450 26.154 455 250 19 6 413 2 417 4.24e-12 67.8
MS.gene72955.t1 MTR_7g056447 28.302 212 108 9 20 225 4 177 8.29e-12 65.9
MS.gene72955.t1 MTR_1g041245 34.146 123 64 3 24 140 10 121 2.99e-11 65.1
MS.gene72955.t1 MTR_2g036470 26.118 425 262 18 12 415 2 395 6.13e-11 64.3
MS.gene72955.t1 MTR_8g014180 55.385 65 18 3 1 64 1 55 9.95e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72955.t1 AT1G16930 26.042 480 248 19 12 416 2 449 2.85e-19 89.7
MS.gene72955.t1 AT1G16930 26.042 480 248 19 12 416 2 449 2.85e-19 89.7
MS.gene72955.t1 AT1G16930 26.042 480 248 19 12 416 2 449 2.85e-19 89.7
MS.gene72955.t1 AT5G56420 25.893 448 245 19 25 416 6 422 4.21e-19 89.4
MS.gene72955.t1 AT5G56420 25.893 448 245 19 25 416 6 422 4.21e-19 89.4
MS.gene72955.t1 AT5G56420 25.893 448 245 19 25 416 6 422 4.21e-19 89.4
MS.gene72955.t1 AT4G26340 26.889 450 239 18 25 416 2 419 5.63e-18 85.9
MS.gene72955.t1 AT5G56370 25.764 458 234 21 25 415 2 420 4.75e-13 70.9
MS.gene72955.t1 AT5G56370 25.764 458 234 21 25 415 2 420 4.75e-13 70.9
MS.gene72955.t1 AT1G66300 25.000 432 250 21 14 413 18 407 4.78e-12 67.8

Find 93 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGACCTATATTCACTTTAA+AGG 0.147428 8.3:+41896760 MS.gene72955:CDS
TTGAAGACAATGCTTATTTC+TGG 0.178189 8.3:+41896844 MS.gene72955:CDS
TTTGGCAATGCAACTTCTAT+TGG 0.243149 8.3:+41896169 MS.gene72955:CDS
GAGGACGCCGTTGACGAATA+TGG 0.282809 8.3:-41895512 None:intergenic
TACCAAAAGAAGAGATTATT+AGG 0.340479 8.3:+41896428 MS.gene72955:intron
ATTCACTTTAAAGGATATAT+AGG 0.344348 8.3:+41896769 MS.gene72955:CDS
ATTCGGAAGATGGGCCAATA+TGG 0.362263 8.3:+41895556 MS.gene72955:CDS
TTGGCAATGCAACTTCTATT+GGG 0.363130 8.3:+41896170 MS.gene72955:CDS
TTCGGCCTCTCTATCGCAAT+CGG 0.367347 8.3:-41895473 None:intergenic
AAGTGGGATACTAGACAATT+AGG 0.367479 8.3:-41896739 None:intergenic
TGCATTGCCAAATGTGATAT+TGG 0.374879 8.3:-41896158 None:intergenic
CCATCGTCTTCTTCTTCCCC+TGG 0.376929 8.3:-41895274 None:intergenic
CCGAATCTTTGGCCAAGCTT+AGG 0.388638 8.3:+41896038 MS.gene72955:CDS
CGAATCTTTGGCCAAGCTTA+GGG 0.393635 8.3:+41896039 MS.gene72955:CDS
AGCCTCACCAATATCACATT+TGG 0.403388 8.3:+41896151 MS.gene72955:CDS
TGCGAAGTACCCGCGACATT+CGG 0.408630 8.3:+41895539 MS.gene72955:CDS
TCGGCCTCTCTATCGCAATC+GGG 0.418289 8.3:-41895472 None:intergenic
ATATGACATAAGAGAGAGTC+TGG 0.419464 8.3:-41895337 None:intergenic
CGTGGATCCGCACCGCCGTT+GGG 0.424276 8.3:+41895611 MS.gene72955:CDS
CTCACAGGTATCTTCATCTC+TGG 0.428747 8.3:+41895410 MS.gene72955:CDS
ACTCTCCTCCAAGCTCTCTC+TGG 0.432658 8.3:+41896268 MS.gene72955:CDS
GTTTAATGCCAGAGAGAGCT+TGG 0.438724 8.3:-41896276 None:intergenic
CCTAAGCTTGGCCAAAGATT+CGG 0.439078 8.3:-41896038 None:intergenic
TGAAGAAATCAAGTCCATAT+TGG 0.439919 8.3:-41895570 None:intergenic
GGCCAAAGATTCGGTTGAAA+AGG 0.442677 8.3:-41896029 None:intergenic
AGAGGAGGTTAGTGCATGAT+AGG 0.446173 8.3:-41895707 None:intergenic
TGAAGCACGTCGTACAGAAA+AGG 0.458324 8.3:-41896539 None:intergenic
CTGTCCAATCTTAGAAACTT+TGG 0.459587 8.3:+41896000 MS.gene72955:intron
CTTGGAGGAGAGTGAGTAAA+GGG 0.461485 8.3:-41896258 None:intergenic
GCACTAACCTCCTCTCCATC+AGG 0.467643 8.3:+41895715 MS.gene72955:CDS
ATTATGAGAGCTTGAAGTAT+AGG 0.479156 8.3:-41896569 None:intergenic
TACCCGCGACATTCGGAAGA+TGG 0.482108 8.3:+41895546 MS.gene72955:CDS
ACGTGGATCCGCACCGCCGT+TGG 0.482549 8.3:+41895610 MS.gene72955:CDS
ATATCCTTTAAAGTGAATAT+AGG 0.491831 8.3:-41896764 None:intergenic
CATCGTAGAAATCAAAAGCT+TGG 0.492563 8.3:-41895441 None:intergenic
ATGTGATATTGGTGAGGCTA+AGG 0.505330 8.3:-41896147 None:intergenic
CGCTCTCTTCACGTCTGAGA+GGG 0.508741 8.3:+41895657 MS.gene72955:CDS
TAACTTAGTCAAATTTAACT+TGG 0.510908 8.3:-41896951 None:intergenic
ATCCTCTAGGTGGGGCCCAA+CGG 0.512937 8.3:-41895626 None:intergenic
GAAGCACGTCGTACAGAAAA+GGG 0.514480 8.3:-41896538 None:intergenic
ACCCGCGACATTCGGAAGAT+GGG 0.520033 8.3:+41895547 MS.gene72955:CDS
CAATGAGTCTTGTTTCTCAC+AGG 0.521437 8.3:+41895395 MS.gene72955:CDS
CTCTAGGTGGGGCCCAACGG+CGG 0.525268 8.3:-41895623 None:intergenic
AGGTTAGTGCATGATAGGAC+CGG 0.527359 8.3:-41895702 None:intergenic
CGGCTTCAAACTCCCTCTCC+CGG 0.528625 8.3:+41895683 MS.gene72955:CDS
GCCCATCTTCCGAATGTCGC+GGG 0.531955 8.3:-41895548 None:intergenic
GCTTGGAGGAGAGTGAGTAA+AGG 0.536107 8.3:-41896259 None:intergenic
ACTGCTTAATCTGTCTGTGT+TGG 0.543571 8.3:-41895311 None:intergenic
TAAAGTGAATATAGGTCAAG+TGG 0.551062 8.3:-41896756 None:intergenic
AGATGTTTCTGTGGGAAGGA+AGG 0.556102 8.3:-41895365 None:intergenic
CGCCGTTGGGCCCCACCTAG+AGG 0.559431 8.3:+41895624 MS.gene72955:CDS
AACAGATTCACTTTCAGAAG+AGG 0.560957 8.3:-41896233 None:intergenic
GTTTCGTTGTTATGCAGCAA+AGG 0.562730 8.3:+41896306 MS.gene72955:CDS
CTTCACGTCTGAGAGGGTGA+CGG 0.566103 8.3:+41895663 MS.gene72955:CDS
AGTTCAAAACGAAGAAAACC+AGG 0.570525 8.3:+41895256 MS.gene72955:CDS
TTAAAGGATATATAGGACAT+TGG 0.572576 8.3:+41896776 MS.gene72955:CDS
GGAAGATGGAACCCTAAGCT+TGG 0.579963 8.3:-41896050 None:intergenic
GTGCATGATAGGACCGGGAG+AGG 0.587136 8.3:-41895696 None:intergenic
TAATGCCAGAGAGAGCTTGG+AGG 0.588199 8.3:-41896273 None:intergenic
TGTACACACAGATGTTTCTG+TGG 0.588924 8.3:-41895374 None:intergenic
GAAGAAGAAGACGATGGTCA+AGG 0.589395 8.3:+41895280 MS.gene72955:CDS
GACTTTGCCATATTCGTCAA+CGG 0.590231 8.3:+41895505 MS.gene72955:CDS
TTTGTTACCTGATGGAGAGG+AGG 0.590784 8.3:-41895722 None:intergenic
GGTGGGGCCCAACGGCGGTG+CGG 0.592704 8.3:-41895618 None:intergenic
TGTCTTGAGATTGACACACC+TGG 0.595030 8.3:+41896115 MS.gene72955:CDS
GAGCGAGATGAAGATCCTCT+AGG 0.596033 8.3:-41895639 None:intergenic
GGCCCATCTTCCGAATGTCG+CGG 0.596944 8.3:-41895549 None:intergenic
CGGCCTCTCTATCGCAATCG+GGG 0.599475 8.3:-41895471 None:intergenic
CGCGGGTACTTCGCATAGAG+AGG 0.601043 8.3:-41895531 None:intergenic
TCGCTCTCTTCACGTCTGAG+AGG 0.602068 8.3:+41895656 MS.gene72955:CDS
AGATTTGTTACCTGATGGAG+AGG 0.603999 8.3:-41895725 None:intergenic
GAGATGAAGATCCTCTAGGT+GGG 0.606924 8.3:-41895635 None:intergenic
TGCATGATAGGACCGGGAGA+GGG 0.607937 8.3:-41895695 None:intergenic
TGGCAGACAGTAGATCCCCT+TGG 0.612624 8.3:-41896931 None:intergenic
GGTTAGTGCATGATAGGACC+GGG 0.613486 8.3:-41895701 None:intergenic
CTGAACGCTCTGTTGACACG+TGG 0.616550 8.3:+41895593 MS.gene72955:CDS
ACACAGATGTTTCTGTGGGA+AGG 0.619305 8.3:-41895369 None:intergenic
AGGCTAAGGTACTTGAGACC+AGG 0.622331 8.3:-41896133 None:intergenic
TACAGAAAAGGGTCGACAGT+GGG 0.624804 8.3:-41896527 None:intergenic
GTACACACAGATGTTTCTGT+GGG 0.628231 8.3:-41895373 None:intergenic
CGAGATGAAGATCCTCTAGG+TGG 0.630001 8.3:-41895636 None:intergenic
ACGAGTTAAATATAGTCTCA+CGG 0.631410 8.3:+41896699 MS.gene72955:CDS
AAAGTGAATATAGGTCAAGT+GGG 0.636598 8.3:-41896755 None:intergenic
AGATGAAGATCCTCTAGGTG+GGG 0.637705 8.3:-41895634 None:intergenic
TTACCCCGATTGCGATAGAG+AGG 0.653960 8.3:+41895468 MS.gene72955:CDS
GTACAGAAAAGGGTCGACAG+TGG 0.663564 8.3:-41896528 None:intergenic
TATTGGGAACTTGCACAACG+TGG 0.670170 8.3:+41896186 MS.gene72955:CDS
TTCAAAACGAAGAAAACCAG+GGG 0.675972 8.3:+41895258 MS.gene72955:CDS
GTTCAAAACGAAGAAAACCA+GGG 0.683634 8.3:+41895257 MS.gene72955:CDS
TGGGAACTTGCACAACGTGG+AGG 0.687506 8.3:+41896189 MS.gene72955:CDS
TGCCAAATGTGATATTGGTG+AGG 0.688345 8.3:-41896153 None:intergenic
TCTCATAATTCATAATGACA+AGG 0.702264 8.3:+41896583 MS.gene72955:CDS
CCAGGGGAAGAAGAAGACGA+TGG 0.730564 8.3:+41895274 MS.gene72955:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTAAATTTAAAACTTTTCA+AGG - chr8.3:41896074-41896093 None:intergenic 15.0%
!!! ATTTTCGAAAAATCTATAAT+AGG - chr8.3:41896461-41896480 None:intergenic 15.0%
!! AAAAAGGTAAAGATTGAAAA+TGG - chr8.3:41895778-41895797 None:intergenic 20.0%
!! AATGGAATATATAATACACA+AGG - chr8.3:41896351-41896370 None:intergenic 20.0%
!! CAAATAATAACTGTACAAAA+AGG - chr8.3:41895794-41895813 None:intergenic 20.0%
!!! ATATCCTTTAAAGTGAATAT+AGG - chr8.3:41896767-41896786 None:intergenic 20.0%
!!! ATTCACTTTAAAGGATATAT+AGG + chr8.3:41896769-41896788 MS.gene72955:CDS 20.0%
!!! TGTGTTTTTGTTTGATTATT+TGG + chr8.3:41896644-41896663 MS.gene72955:intron 20.0%
! TACCAAAAGAAGAGATTATT+AGG + chr8.3:41896428-41896447 MS.gene72955:intron 25.0%
! TCTCATAATTCATAATGACA+AGG + chr8.3:41896583-41896602 MS.gene72955:CDS 25.0%
! TTAAAGGATATATAGGACAT+TGG + chr8.3:41896776-41896795 MS.gene72955:CDS 25.0%
! TTGACCTATATTCACTTTAA+AGG + chr8.3:41896760-41896779 MS.gene72955:CDS 25.0%
!! AAAATTTTCGAATATGCCAA+GGG + chr8.3:41896915-41896934 MS.gene72955:CDS 25.0%
!! TACCTAATAATCTCTTCTTT+TGG - chr8.3:41896433-41896452 None:intergenic 25.0%
!! TTTAACTTAAGCTTTAGTGT+GGG + chr8.3:41896619-41896638 MS.gene72955:intron 25.0%
!!! TTAAAACTTTTCAAGGAAGA+TGG - chr8.3:41896067-41896086 None:intergenic 25.0%
!!! TTTTTCGAAAATGCACTTTT+CGG + chr8.3:41896469-41896488 MS.gene72955:CDS 25.0%
AAAGTGAATATAGGTCAAGT+GGG - chr8.3:41896758-41896777 None:intergenic 30.0%
AAGACACTACAACTAGAAAT+TGG + chr8.3:41895938-41895957 MS.gene72955:intron 30.0%
ACGAGTTAAATATAGTCTCA+CGG + chr8.3:41896699-41896718 MS.gene72955:CDS 30.0%
ATTATGAGAGCTTGAAGTAT+AGG - chr8.3:41896572-41896591 None:intergenic 30.0%
TAAAGTGAATATAGGTCAAG+TGG - chr8.3:41896759-41896778 None:intergenic 30.0%
TCTCTATGGTGAAATATTTG+TGG + chr8.3:41895877-41895896 MS.gene72955:intron 30.0%
TGAAGAAATCAAGTCCATAT+TGG - chr8.3:41895573-41895592 None:intergenic 30.0%
TGGTGAAATATTTGTGGAAT+TGG + chr8.3:41895883-41895902 MS.gene72955:intron 30.0%
TTGAAGACAATGCTTATTTC+TGG + chr8.3:41896844-41896863 MS.gene72955:CDS 30.0%
! AAATTTTCGAATATGCCAAG+GGG + chr8.3:41896916-41896935 MS.gene72955:CDS 30.0%
! AGACAATTAGGAACACTTTT+CGG - chr8.3:41896730-41896749 None:intergenic 30.0%
! GAAAATTTTCGAATATGCCA+AGG + chr8.3:41896914-41896933 MS.gene72955:CDS 30.0%
! GTTTAACTTAAGCTTTAGTG+TGG + chr8.3:41896618-41896637 MS.gene72955:intron 30.0%
!! ACAAAAGATTTGTTACCTGA+TGG - chr8.3:41895733-41895752 None:intergenic 30.0%
!! ACAACTAGAAATTGGTAATG+TGG + chr8.3:41895946-41895965 MS.gene72955:intron 30.0%
!! AGTTCCAAAGTTTCTAAGAT+TGG - chr8.3:41896007-41896026 None:intergenic 30.0%
!! AGTTGTAATTTGTTTCCTAC+AGG + chr8.3:41896669-41896688 MS.gene72955:intron 30.0%
!!! GGCAAAGTCTTTGAAATTTT+CGG - chr8.3:41895494-41895513 None:intergenic 30.0%
AACAGATTCACTTTCAGAAG+AGG - chr8.3:41896236-41896255 None:intergenic 35.0%
AAGTGGGATACTAGACAATT+AGG - chr8.3:41896742-41896761 None:intergenic 35.0%
AGTTCAAAACGAAGAAAACC+AGG + chr8.3:41895256-41895275 MS.gene72955:CDS 35.0%
AGTTGTAGTGTCTTCAATGA+TGG - chr8.3:41895932-41895951 None:intergenic 35.0%
ATATGACATAAGAGAGAGTC+TGG - chr8.3:41895340-41895359 None:intergenic 35.0%
CATCGTAGAAATCAAAAGCT+TGG - chr8.3:41895444-41895463 None:intergenic 35.0%
CTGTCCAATCTTAGAAACTT+TGG + chr8.3:41896000-41896019 MS.gene72955:intron 35.0%
GTTCAAAACGAAGAAAACCA+GGG + chr8.3:41895257-41895276 MS.gene72955:CDS 35.0%
TGCATTGCCAAATGTGATAT+TGG - chr8.3:41896161-41896180 None:intergenic 35.0%
TTCAAAACGAAGAAAACCAG+GGG + chr8.3:41895258-41895277 MS.gene72955:CDS 35.0%
TTGGCAATGCAACTTCTATT+GGG + chr8.3:41896170-41896189 MS.gene72955:CDS 35.0%
TTTGGCAATGCAACTTCTAT+TGG + chr8.3:41896169-41896188 MS.gene72955:CDS 35.0%
! GCTAGTTATGTTTTGCAGTA+TGG + chr8.3:41896814-41896833 MS.gene72955:CDS 35.0%
! TTCCTTTTCAACCGAATCTT+TGG + chr8.3:41896027-41896046 MS.gene72955:CDS 35.0%
!! CAAAGTTTCTAAGATTGGAC+AGG - chr8.3:41896002-41896021 None:intergenic 35.0%
ACTGCTTAATCTGTCTGTGT+TGG - chr8.3:41895314-41895333 None:intergenic 40.0%
AGCCTCACCAATATCACATT+TGG + chr8.3:41896151-41896170 MS.gene72955:CDS 40.0%
ATGTGATATTGGTGAGGCTA+AGG - chr8.3:41896150-41896169 None:intergenic 40.0%
CTAATTCTCGTATCTCCACT+TGG + chr8.3:41896391-41896410 MS.gene72955:intron 40.0%
GACTTTGCCATATTCGTCAA+CGG + chr8.3:41895505-41895524 MS.gene72955:CDS 40.0%
GTTAACGTACACAGTCTCTA+TGG + chr8.3:41895863-41895882 MS.gene72955:intron 40.0%
GTTTCGTTGTTATGCAGCAA+AGG + chr8.3:41896306-41896325 MS.gene72955:CDS 40.0%
TAACTCGTGATGTATCCTGT+AGG - chr8.3:41896687-41896706 None:intergenic 40.0%
TGCCAAATGTGATATTGGTG+AGG - chr8.3:41896156-41896175 None:intergenic 40.0%
! GTACACACAGATGTTTCTGT+GGG - chr8.3:41895376-41895395 None:intergenic 40.0%
! TGTACACACAGATGTTTCTG+TGG - chr8.3:41895377-41895396 None:intergenic 40.0%
!! AGATTTGTTACCTGATGGAG+AGG - chr8.3:41895728-41895747 None:intergenic 40.0%
!! CAATGAGTCTTGTTTCTCAC+AGG + chr8.3:41895395-41895414 MS.gene72955:CDS 40.0%
ACGTACACAACAACACCAAG+TGG - chr8.3:41896409-41896428 None:intergenic 45.0%
AGAGGAGGTTAGTGCATGAT+AGG - chr8.3:41895710-41895729 None:intergenic 45.0%
AGATGAAGATCCTCTAGGTG+GGG - chr8.3:41895637-41895656 None:intergenic 45.0%
AGGTTAGTGCATGATAGGAC+CGG - chr8.3:41895705-41895724 None:intergenic 45.0%
ATTCGGAAGATGGGCCAATA+TGG + chr8.3:41895556-41895575 MS.gene72955:CDS 45.0%
CCTAAGCTTGGCCAAAGATT+CGG - chr8.3:41896041-41896060 None:intergenic 45.0%
CGAATCTTTGGCCAAGCTTA+GGG + chr8.3:41896039-41896058 MS.gene72955:CDS 45.0%
CTCACAGGTATCTTCATCTC+TGG + chr8.3:41895410-41895429 MS.gene72955:CDS 45.0%
CTTGGAGGAGAGTGAGTAAA+GGG - chr8.3:41896261-41896280 None:intergenic 45.0%
GAAGAAGAAGACGATGGTCA+AGG + chr8.3:41895280-41895299 MS.gene72955:CDS 45.0%
GAAGCACGTCGTACAGAAAA+GGG - chr8.3:41896541-41896560 None:intergenic 45.0%
GAGATGAAGATCCTCTAGGT+GGG - chr8.3:41895638-41895657 None:intergenic 45.0%
GGCAGAGAGAAGGATATCAA+CGG - chr8.3:41895981-41896000 None:intergenic 45.0%
GTTTAATGCCAGAGAGAGCT+TGG - chr8.3:41896279-41896298 None:intergenic 45.0%
TACAGAAAAGGGTCGACAGT+GGG - chr8.3:41896530-41896549 None:intergenic 45.0%
TATTGGGAACTTGCACAACG+TGG + chr8.3:41896186-41896205 MS.gene72955:CDS 45.0%
TGAAGCACGTCGTACAGAAA+AGG - chr8.3:41896542-41896561 None:intergenic 45.0%
TGTCTTGAGATTGACACACC+TGG + chr8.3:41896115-41896134 MS.gene72955:CDS 45.0%
TTTGTTACCTGATGGAGAGG+AGG - chr8.3:41895725-41895744 None:intergenic 45.0%
! ACACAGATGTTTCTGTGGGA+AGG - chr8.3:41895372-41895391 None:intergenic 45.0%
! AGATGTTTCTGTGGGAAGGA+AGG - chr8.3:41895368-41895387 None:intergenic 45.0%
! GGCCAAAGATTCGGTTGAAA+AGG - chr8.3:41896032-41896051 None:intergenic 45.0%
!!! CAAATTTTTTAAATATATAA+TGG - chr8.3:41896369-41896388 None:intergenic 5.0%
AGATTGGACAGGCAGAGAGA+AGG - chr8.3:41895991-41896010 None:intergenic 50.0%
CCGAATCTTTGGCCAAGCTT+AGG + chr8.3:41896038-41896057 MS.gene72955:CDS 50.0%
CGAGATGAAGATCCTCTAGG+TGG - chr8.3:41895639-41895658 None:intergenic 50.0%
GAGCGAGATGAAGATCCTCT+AGG - chr8.3:41895642-41895661 None:intergenic 50.0%
GCTTGGAGGAGAGTGAGTAA+AGG - chr8.3:41896262-41896281 None:intergenic 50.0%
GGAAGATGGAACCCTAAGCT+TGG - chr8.3:41896053-41896072 None:intergenic 50.0%
GGTTAGTGCATGATAGGACC+GGG - chr8.3:41895704-41895723 None:intergenic 50.0%
GTACAGAAAAGGGTCGACAG+TGG - chr8.3:41896531-41896550 None:intergenic 50.0%
TAATGCCAGAGAGAGCTTGG+AGG - chr8.3:41896276-41896295 None:intergenic 50.0%
TTACCCCGATTGCGATAGAG+AGG + chr8.3:41895468-41895487 MS.gene72955:CDS 50.0%
TTCGGCCTCTCTATCGCAAT+CGG - chr8.3:41895476-41895495 None:intergenic 50.0%
! AGGCTAAGGTACTTGAGACC+AGG - chr8.3:41896136-41896155 None:intergenic 50.0%
ACCCGCGACATTCGGAAGAT+GGG + chr8.3:41895547-41895566 MS.gene72955:CDS 55.0%
ACTCTCCTCCAAGCTCTCTC+TGG + chr8.3:41896268-41896287 MS.gene72955:CDS 55.0%
CCAGGGGAAGAAGAAGACGA+TGG + chr8.3:41895274-41895293 MS.gene72955:CDS 55.0%
CCATCGTCTTCTTCTTCCCC+TGG - chr8.3:41895277-41895296 None:intergenic 55.0%
CGCTCTCTTCACGTCTGAGA+GGG + chr8.3:41895657-41895676 MS.gene72955:CDS 55.0%
CTGAACGCTCTGTTGACACG+TGG + chr8.3:41895593-41895612 MS.gene72955:CDS 55.0%
CTTCACGTCTGAGAGGGTGA+CGG + chr8.3:41895663-41895682 MS.gene72955:CDS 55.0%
GAGGACGCCGTTGACGAATA+TGG - chr8.3:41895515-41895534 None:intergenic 55.0%
GCACTAACCTCCTCTCCATC+AGG + chr8.3:41895715-41895734 MS.gene72955:CDS 55.0%
TACCCGCGACATTCGGAAGA+TGG + chr8.3:41895546-41895565 MS.gene72955:CDS 55.0%
TCGCTCTCTTCACGTCTGAG+AGG + chr8.3:41895656-41895675 MS.gene72955:CDS 55.0%
TCGGCCTCTCTATCGCAATC+GGG - chr8.3:41895475-41895494 None:intergenic 55.0%
TGCATGATAGGACCGGGAGA+GGG - chr8.3:41895698-41895717 None:intergenic 55.0%
TGGCAGACAGTAGATCCCCT+TGG - chr8.3:41896934-41896953 None:intergenic 55.0%
TGGGAACTTGCACAACGTGG+AGG + chr8.3:41896189-41896208 MS.gene72955:CDS 55.0%
! TGCGAAGTACCCGCGACATT+CGG + chr8.3:41895539-41895558 MS.gene72955:CDS 55.0%
ATCCTCTAGGTGGGGCCCAA+CGG - chr8.3:41895629-41895648 None:intergenic 60.0%
CGCGGGTACTTCGCATAGAG+AGG - chr8.3:41895534-41895553 None:intergenic 60.0%
CGGCCTCTCTATCGCAATCG+GGG - chr8.3:41895474-41895493 None:intergenic 60.0%
CGGCTTCAAACTCCCTCTCC+CGG + chr8.3:41895683-41895702 MS.gene72955:CDS 60.0%
GCCCATCTTCCGAATGTCGC+GGG - chr8.3:41895551-41895570 None:intergenic 60.0%
GGCCCATCTTCCGAATGTCG+CGG - chr8.3:41895552-41895571 None:intergenic 60.0%
GTGCATGATAGGACCGGGAG+AGG - chr8.3:41895699-41895718 None:intergenic 60.0%
ACGTGGATCCGCACCGCCGT+TGG + chr8.3:41895610-41895629 MS.gene72955:CDS 70.0%
CGTGGATCCGCACCGCCGTT+GGG + chr8.3:41895611-41895630 MS.gene72955:CDS 70.0%
CTCTAGGTGGGGCCCAACGG+CGG - chr8.3:41895626-41895645 None:intergenic 70.0%
CGCCGTTGGGCCCCACCTAG+AGG + chr8.3:41895624-41895643 MS.gene72955:CDS 75.0%
! GGTGGGGCCCAACGGCGGTG+CGG - chr8.3:41895621-41895640 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 41895247 41896969 41895247 ID=MS.gene72955
chr8.3 mRNA 41895247 41896969 41895247 ID=MS.gene72955.t1;Parent=MS.gene72955
chr8.3 exon 41895247 41895736 41895247 ID=MS.gene72955.t1.exon1;Parent=MS.gene72955.t1
chr8.3 CDS 41895247 41895736 41895247 ID=cds.MS.gene72955.t1;Parent=MS.gene72955.t1
chr8.3 exon 41896014 41896327 41896014 ID=MS.gene72955.t1.exon2;Parent=MS.gene72955.t1
chr8.3 CDS 41896014 41896327 41896014 ID=cds.MS.gene72955.t1;Parent=MS.gene72955.t1
chr8.3 exon 41896437 41896604 41896437 ID=MS.gene72955.t1.exon3;Parent=MS.gene72955.t1
chr8.3 CDS 41896437 41896604 41896437 ID=cds.MS.gene72955.t1;Parent=MS.gene72955.t1
chr8.3 exon 41896691 41896969 41896691 ID=MS.gene72955.t1.exon4;Parent=MS.gene72955.t1
chr8.3 CDS 41896691 41896969 41896691 ID=cds.MS.gene72955.t1;Parent=MS.gene72955.t1
Gene Sequence

>MS.gene72955

ATGGCGGAAAGTTCAAAACGAAGAAAACCAGGGGAAGAAGAAGACGATGGTCAAGGCAGTAACACCAACACAGACAGATTAAGCAGTCTGCCAGACTCTCTCTTATGTCATATACTTTCCTTCCTTCCCACAGAAACATCTGTGTGTACAATGAGTCTTGTTTCTCACAGGTATCTTCATCTCTGGAAACATCTCCAAGCTTTTGATTTCTACGATGTGTATTACCCCGATTGCGATAGAGAGGCCGAAAATTTCAAAGACTTTGCCATATTCGTCAACGGCGTCCTCTCTATGCGAAGTACCCGCGACATTCGGAAGATGGGCCAATATGGACTTGATTTCTTCACTGAACGCTCTGTTGACACGTGGATCCGCACCGCCGTTGGGCCCCACCTAGAGGATCTTCATCTCGCTCTCTTCACGTCTGAGAGGGTGACGGCTTCAAACTCCCTCTCCCGGTCCTATCATGCACTAACCTCCTCTCCATCAGGTAACAAATCTTTTGTCAAACAATCTTCTATTTGCATGCCATTTTCAATCTTTACCTTTTTGTACAGTTATTATTTGCACAGTTATTCTTGTTTAAATTAAAATGTGACATAATAAATGAAGATTTGTTAACGTACACAGTCTCTATGGTGAAATATTTGTGGAATTGGAACAATCTTCGCGTGTTTGTTTACCATCATTGAAGACACTACAACTAGAAATTGGTAATGTGGATGTGAATTCCGTTGATATCCTTCTCTCTGCCTGTCCAATCTTAGAAACTTTGGAACTTTCCTTTTCAACCGAATCTTTGGCCAAGCTTAGGGTTCCATCTTCCTTGAAAAGTTTTAAATTTACTATAGAGAATGACACTGAAGTTTGTCTTGAGATTGACACACCTGGTCTCAAGTACCTTAGCCTCACCAATATCACATTTGGCAATGCAACTTCTATTGGGAACTTGCACAACGTGGAGGAAGCATATCTTGATGTACTCTCCTCTTCTGAAAGTGAATCTGTTGACCCTTTACTCACTCTCCTCCAAGCTCTCTCTGGCATTAAACGTCTAGTGTTTCGTTGTTATGCAGCAAAGGTGAGTGAAATTTTCATTATCCTTGTGTATTATATATTCCATTATATATTTAAAAAATTTGTGCTAATTCTCGTATCTCCACTTGGTGTTGTTGTGTACGTACCAAAAGAAGAGATTATTAGGTACTGAACCTATTATAGATTTTTCGAAAATGCACTTTTCGGAATTTCGCTGTTTACTACATCTAGAGCTCATTTTTCCCACTGTCGACCCTTTTCTGTACGACGTGCTTCAGAAATGTCCTATACTTCAAGCTCTCATAATTCATAATGACAAGGTTCGTAAATAGAGTTTAACTTAAGCTTTAGTGTGGGTGTGTGTTTTTGTTTGATTATTTGGTGAGTTGTAATTTGTTTCCTACAGGATACATCACGAGTTAAATATAGTCTCACGGAAAAGCCGAAAAGTGTTCCTAATTGTCTAGTATCCCACTTGACCTATATTCACTTTAAAGGATATATAGGACATTGGCATGAGAAAGAATTTGCTAGTTATGTTTTGCAGTATGGACTTGTTTTGAAGACAATGCTTATTTCTGGTTTTTTGTCGAATGAAACGAAGAAGCATGCTAAGTACAACTATCGCAGAAAATTTTCGAATATGCCAAGGGGATCTACTGTCTGCCAAGTTAAATTTGACTAA
Protein sequence

>MS.gene72955.t1

MAESSKRRKPGEEEDDGQGSNTNTDRLSSLPDSLLCHILSFLPTETSVCTMSLVSHRYLHLWKHLQAFDFYDVYYPDCDREAENFKDFAIFVNGVLSMRSTRDIRKMGQYGLDFFTERSVDTWIRTAVGPHLEDLHLALFTSERVTASNSLSRSYHALTSSPSETLELSFSTESLAKLRVPSSLKSFKFTIENDTEVCLEIDTPGLKYLSLTNITFGNATSIGNLHNVEEAYLDVLSSSESESVDPLLTLLQALSGIKRLVFRCYAAKKRLLGTEPIIDFSKMHFSEFRCLLHLELIFPTVDPFLYDVLQKCPILQALIIHNDKDTSRVKYSLTEKPKSVPNCLVSHLTYIHFKGYIGHWHEKEFASYVLQYGLVLKTMLISGFLSNETKKHAKYNYRRKFSNMPRGSTVCQVKFD