Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63095.t1 | AET03063.1 | 88.4 | 414 | 23 | 1 | 1 | 389 | 41 | 454 | 2.00E-210 | 741.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63095.t1 | Q9FZ52 | 25.4 | 437 | 243 | 18 | 2 | 386 | 41 | 446 | 6.9e-18 | 93.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63095.t1 | G7LEL8 | 88.4 | 414 | 23 | 1 | 1 | 389 | 41 | 454 | 1.4e-210 | 741.5 |
TFs/TRs:
Protein Kinases:
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| No data available in table | ||||
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene002108 | MS.gene63095 | PPI |
| MS.gene005794 | MS.gene63095 | PPI |
| MS.gene006692 | MS.gene63095 | PPI |
| MS.gene006727 | MS.gene63095 | PPI |
| MS.gene006730 | MS.gene63095 | PPI |
| MS.gene011055 | MS.gene63095 | PPI |
| MS.gene014840 | MS.gene63095 | PPI |
| MS.gene021114 | MS.gene63095 | PPI |
| MS.gene022905 | MS.gene63095 | PPI |
| MS.gene022909 | MS.gene63095 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63095.t1 | MTR_8g062270 | 88.406 | 414 | 23 | 1 | 1 | 389 | 41 | 454 | 0.0 | 741 |
| MS.gene63095.t1 | MTR_8g468760 | 61.244 | 418 | 99 | 8 | 1 | 388 | 34 | 418 | 1.93e-162 | 462 |
| MS.gene63095.t1 | MTR_5g092960 | 57.957 | 421 | 136 | 7 | 3 | 388 | 47 | 461 | 4.72e-152 | 437 |
| MS.gene63095.t1 | MTR_8g468840 | 56.971 | 416 | 142 | 8 | 2 | 387 | 40 | 448 | 8.62e-148 | 426 |
| MS.gene63095.t1 | MTR_8g467800 | 51.914 | 418 | 159 | 8 | 2 | 388 | 46 | 452 | 9.50e-128 | 375 |
| MS.gene63095.t1 | MTR_2g083840 | 48.095 | 420 | 172 | 7 | 3 | 388 | 49 | 456 | 9.37e-116 | 345 |
| MS.gene63095.t1 | MTR_4g032730 | 47.222 | 432 | 174 | 9 | 3 | 388 | 43 | 466 | 8.87e-114 | 340 |
| MS.gene63095.t1 | MTR_5g092960 | 57.846 | 325 | 101 | 5 | 3 | 294 | 47 | 368 | 1.28e-111 | 332 |
| MS.gene63095.t1 | MTR_8g468810 | 45.561 | 428 | 177 | 12 | 1 | 388 | 51 | 462 | 4.90e-109 | 328 |
| MS.gene63095.t1 | MTR_8g058970 | 46.861 | 446 | 165 | 14 | 1 | 388 | 45 | 476 | 2.39e-104 | 316 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63095.t1 | AT5G53840 | 24.703 | 421 | 254 | 16 | 2 | 386 | 44 | 437 | 2.26e-22 | 98.6 |
| MS.gene63095.t1 | AT1G16930 | 25.400 | 437 | 243 | 18 | 2 | 386 | 41 | 446 | 1.73e-18 | 87.0 |
| MS.gene63095.t1 | AT1G16930 | 25.400 | 437 | 243 | 18 | 2 | 386 | 41 | 446 | 1.73e-18 | 87.0 |
| MS.gene63095.t1 | AT1G16930 | 25.400 | 437 | 243 | 18 | 2 | 386 | 41 | 446 | 1.73e-18 | 87.0 |
| MS.gene63095.t1 | AT5G53635 | 24.766 | 428 | 253 | 15 | 3 | 389 | 27 | 426 | 1.26e-16 | 81.6 |
| MS.gene63095.t1 | AT5G38570 | 27.247 | 356 | 207 | 17 | 46 | 388 | 59 | 375 | 7.50e-16 | 79.0 |
| MS.gene63095.t1 | AT1G78750 | 24.153 | 443 | 253 | 18 | 2 | 389 | 44 | 458 | 1.19e-15 | 78.6 |
| MS.gene63095.t1 | AT1G78750 | 24.153 | 443 | 253 | 18 | 2 | 389 | 44 | 458 | 1.19e-15 | 78.6 |
| MS.gene63095.t1 | AT1G13780 | 23.756 | 442 | 255 | 16 | 2 | 386 | 36 | 452 | 1.52e-15 | 78.6 |
| MS.gene63095.t1 | AT5G56700 | 26.074 | 349 | 197 | 15 | 46 | 356 | 22 | 347 | 1.78e-15 | 77.4 |
Find 102 sgRNAs with CRISPR-Local
Find 147 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATGAGCTGGAATTTATT+GGG | 0.368042 | 8.1:-42915927 | MS.gene63095:CDS |
| AAAATTTCAGATCTACCAAG+AGG | 0.633493 | 8.1:-42915823 | MS.gene63095:CDS |
| AAACTCCGGAAAATGTAAAA+TGG | 0.241590 | 8.1:+42916264 | None:intergenic |
| AAATGGGATATGAGGCATTT+AGG | 0.311419 | 8.1:+42915979 | None:intergenic |
| AACAGATTCACATTTAGGAG+TGG | 0.595892 | 8.1:+42916470 | None:intergenic |
| AACTCCGGAAAATGTAAAAT+GGG | 0.406590 | 8.1:+42916265 | None:intergenic |
| AAGAGAAGAGTGTGAGAGGT+AGG | 0.423108 | 8.1:+42917148 | None:intergenic |
| AAGCATAGAATGGATCGTTT+GGG | 0.488136 | 8.1:+42917259 | None:intergenic |
| AATCTCCTTCGAGCTCTTTC+CGG | 0.331425 | 8.1:-42916435 | MS.gene63095:CDS |
| ACAGATTCACATTTAGGAGT+GGG | 0.547187 | 8.1:+42916471 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATTTCAGATCTACCAAG+AGG | - | chr8.1:42917434-42917453 | MS.gene63095:CDS | 30.0% | |
| AAACTCCGGAAAATGTAAAA+TGG | + | chr8.1:42916996-42917015 | None:intergenic | 30.0% | |
| AACTCCGGAAAATGTAAAAT+GGG | + | chr8.1:42916995-42917014 | None:intergenic | 30.0% | |
| ACATCCGTATAAGTGAATAA+AGG | + | chr8.1:42917306-42917325 | None:intergenic | 30.0% | |
| CTGAATCTTTGGACATAATT+CGG | - | chr8.1:42916592-42916611 | MS.gene63095:CDS | 30.0% | |
| GAAAATGAGCTGGAATTTAT+TGG | - | chr8.1:42917329-42917348 | MS.gene63095:CDS | 30.0% | |
| TAAAGTTAAACTACTTCCTC+CGG | + | chr8.1:42916321-42916340 | None:intergenic | 30.0% | |
| TCTCTTATATATAATACCGG+AGG | - | chr8.1:42916302-42916321 | MS.gene63095:intron | 30.0% | |
| TGAATCTTTGGACATAATTC+GGG | - | chr8.1:42916593-42916612 | MS.gene63095:CDS | 30.0% | |
| TTTCTTTAGTCCTGAATCTT+TGG | - | chr8.1:42916581-42916600 | MS.gene63095:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 42915792 | 42917487 | 42915792 | ID=MS.gene63095 |
| chr8.1 | mRNA | 42915792 | 42917487 | 42915792 | ID=MS.gene63095.t1;Parent=MS.gene63095 |
| chr8.1 | exon | 42917137 | 42917487 | 42917137 | ID=MS.gene63095.t1.exon1;Parent=MS.gene63095.t1 |
| chr8.1 | CDS | 42917137 | 42917487 | 42917137 | ID=cds.MS.gene63095.t1;Parent=MS.gene63095.t1 |
| chr8.1 | exon | 42916398 | 42916787 | 42916398 | ID=MS.gene63095.t1.exon2;Parent=MS.gene63095.t1 |
| chr8.1 | CDS | 42916398 | 42916787 | 42916398 | ID=cds.MS.gene63095.t1;Parent=MS.gene63095.t1 |
| chr8.1 | exon | 42916144 | 42916302 | 42916144 | ID=MS.gene63095.t1.exon3;Parent=MS.gene63095.t1 |
| chr8.1 | CDS | 42916144 | 42916302 | 42916144 | ID=cds.MS.gene63095.t1;Parent=MS.gene63095.t1 |
| chr8.1 | exon | 42915792 | 42916061 | 42915792 | ID=MS.gene63095.t1.exon4;Parent=MS.gene63095.t1 |
| chr8.1 | CDS | 42915792 | 42916061 | 42915792 | ID=cds.MS.gene63095.t1;Parent=MS.gene63095.t1 |
| Gene Sequence |
>MS.gene63095 ATGAGCCTAGTCTCCCGCAAATGGCGCAACCTCTGGAAGAATCTCGAAGTCTTCGATTTCCGCGACAAATGCAATGAATACAGCTACCAAGCCCCCGACGACGAATTCAACGAACAATTCATGTTGTTCACGGTTTTCGTCAACACTGTCCTCGCTCTCCGTCGCTCACGCGTCGTTCGGAAGTTCCGTCTCTCCTCCGACCACATCCCAAACGATCCATTCTATGCTTACTCAGTCGACACGTGGCTCAGTATCGCCATTGGGCCCCACCTCCAGGAATTTCATCTCACCCTCTTTACTGCCGGCGCCTTCACCAACCTACCTCTCACACTCTTCTCTTGCTCTAACCTTGTCTCTCTCAGGCATGCAACAATTCCTATTTCATCCTCTTTTCTTTATTTTTGTTTTCTGCATTCCATTGTTATTGTCTAGGGTTAGAGAAAATTCGAAAGTTGATGTATCCGGTTCAAAATTTGGATCGGATACATCAACTTTTTGTTAACCCAATTTTCTCTTATATATAATACCGGAGGAAGTAGTTTAACTTTAATTTTTATGCTTGTTCAATTTAGCTGTTTTGATTTAATTTAAATGATGATAACTTAACATAACACATTCATGAATGTAATGTAACAGTCTCAGTGGATATATATTGTTGCCATTGCAAGACCCTTCGGAAATTTGCTTACCGTCGTTGAAGGTGCTGGAACTACTACAAATGCATCACTTGAATTTGAGTTCCATGCATATCCTTCTCTCTGCCTGTCCTGTTCTCGAGAATCTGGAACTTTTCTTTAGTCCTGAATCTTTGGACATAATTCGGGTGCCATCTTCCTTGAAGAAGTTAAAAATCACTGTTGATAATAACGTTGGGGCTTGGCTTGAAATAGATGCACCTGGTCTCAAGTACCTTAGCCTTGAAGGGATCACATTCCGTGATGCCGCGGCTGTTGGGAACTTGCACAGCGTGGAAGAAGCATATCTTGATGTATTTCCCACTCCTAAATGTGAATCTGTTGAGCCTTTACTCAATCTCCTTCGAGCTCTTTCCGGAATTAAACGTCTAGAACTACATAATTCAACCCCAAAGGTGACTGAAGTTTTCATTATTTGTAGTATATGTTGTTTTGTAGTAATAATTATTAAATAATCATTTAATCACTTGCTTTTGCCGTGTACAAGCAGTGGCTATTTGCTGCCCCCATTTTACATTTTCCGGAGTTTCACTATTTGCTTCATCTAAAGCTTAAGTATCCCCCTTTCAACTCAACTTTTTTGTTCGACGTGCTTCAAAAATGTCCAATGCTAGAAACTCTCATAGCATTTATGTTCGACATCGACATGGTTTGTCCAGTTTAACTCGAGCTTTGTTGCGTTCGTGTTTTTTTTGTTTGGATCTTATATATCAGTTGCTCCTTGTTGCCAGGTGGATCGATCATTTGATTCGGTGCCTTCATATAGATGGGAGGCAAAGTCTAAAAGTGTTCCTAAATGCCTCATATCCCATTTGACCTTTATTCACTTATACGGATGTCCAGAAAATGAGCTGGAATTTATTGGGTATATTTTGCAACATGGACTTGTTTTGAAGACAATGATTGTTGATGAATATTGGCGAGACCAACCAGACCGCTGGATGAAAAAAATTTCAGATCTACCAAGAGGATCTGCCATGTGCCAAGTTAAATTTCTCTGA |
| Protein sequence |
>MS.gene63095.t1 MSLVSRKWRNLWKNLEVFDFRDKCNEYSYQAPDDEFNEQFMLFTVFVNTVLALRRSRVVRKFRLSSDHIPNDPFYAYSVDTWLSIAIGPHLQEFHLTLFTAGAFTNLPLTLFSCSNLVLELLQMHHLNLSSMHILLSACPVLENLELFFSPESLDIIRVPSSLKKLKITVDNNVGAWLEIDAPGLKYLSLEGITFRDAAAVGNLHSVEEAYLDVFPTPKCESVEPLLNLLRALSGIKRLELHNSTPKWLFAAPILHFPEFHYLLHLKLKYPPFNSTFLFDVLQKCPMLETLIAFMFDIDMVDRSFDSVPSYRWEAKSKSVPKCLISHLTFIHLYGCPENELEFIGYILQHGLVLKTMIVDEYWRDQPDRWMKKISDLPRGSAMCQVKFL |
