AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25414

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25414.t1 MTR_8g468810 78.795 415 85 2 1 414 51 463 0.0 653
MS.gene25414.t1 MTR_8g058970 63.048 433 141 3 1 414 45 477 0.0 522
MS.gene25414.t1 MTR_8g468810 81.424 323 57 2 1 322 51 371 0.0 519
MS.gene25414.t1 MTR_8g468810 82.129 263 45 1 1 263 51 311 7.23e-151 430
MS.gene25414.t1 MTR_8g468810 82.129 263 45 1 1 263 51 311 1.36e-150 430
MS.gene25414.t1 MTR_8g468810 82.129 263 45 1 1 263 51 311 2.11e-150 430
MS.gene25414.t1 MTR_2g083840 53.828 418 182 6 1 414 47 457 2.57e-140 408
MS.gene25414.t1 MTR_8g062270 47.664 428 194 12 1 413 41 453 4.58e-114 341
MS.gene25414.t1 MTR_4g032730 46.136 440 192 9 3 413 43 466 5.30e-113 339
MS.gene25414.t1 MTR_5g092960 49.302 430 184 13 3 413 47 461 5.04e-108 326
MS.gene25414.t1 MTR_8g468840 47.143 420 202 9 2 412 40 448 7.87e-108 325
MS.gene25414.t1 MTR_8g468760 48.244 427 165 13 1 413 34 418 1.43e-105 318
MS.gene25414.t1 MTR_8g467800 47.541 427 191 12 2 414 46 453 4.34e-105 318
MS.gene25414.t1 MTR_8g014260 43.499 423 212 15 4 414 44 451 1.21e-96 296
MS.gene25414.t1 MTR_8g445780 44.536 366 181 9 4 364 41 389 1.61e-82 258
MS.gene25414.t1 MTR_5g092960 50.000 334 144 10 3 322 47 371 5.66e-78 247
MS.gene25414.t1 MTR_8g469020 37.875 433 234 12 2 414 34 451 1.10e-76 245
MS.gene25414.t1 MTR_8g058965 39.019 428 235 10 2 414 40 456 1.21e-75 243
MS.gene25414.t1 MTR_8g070950 37.875 433 211 15 1 414 41 434 1.62e-70 229
MS.gene25414.t1 MTR_4g050250 41.099 382 189 10 1 356 47 418 1.48e-69 226
MS.gene25414.t1 MTR_8g467800 51.111 270 120 6 2 266 46 308 4.00e-66 214
MS.gene25414.t1 MTR_8g062080 36.557 424 232 13 1 414 49 445 2.54e-63 210
MS.gene25414.t1 MTR_8g062010 35.817 416 245 11 1 414 35 430 1.92e-61 205
MS.gene25414.t1 MTR_8g014160 33.649 422 208 11 2 414 67 425 2.54e-59 199
MS.gene25414.t1 MTR_4g024710 42.193 301 160 8 117 414 58 347 1.21e-58 195
MS.gene25414.t1 MTR_8g062310 35.024 414 227 13 4 414 54 428 1.19e-57 195
MS.gene25414.t1 MTR_8g062300 34.744 449 235 16 1 414 52 477 9.80e-55 188
MS.gene25414.t1 MTR_4g024745 41.259 286 154 8 115 397 10 284 1.55e-48 167
MS.gene25414.t1 MTR_8g014210 43.568 241 126 6 174 414 1 231 2.33e-46 159
MS.gene25414.t1 MTR_8g467790 33.875 369 178 13 1 354 174 491 2.09e-43 159
MS.gene25414.t1 MTR_8g077460 42.564 195 102 5 4 191 38 229 9.73e-37 138
MS.gene25414.t1 MTR_8g077460 33.209 268 150 9 155 414 129 375 2.44e-29 117
MS.gene25414.t1 MTR_5g021550 50.862 116 54 3 4 116 47 162 1.00e-28 110
MS.gene25414.t1 MTR_8g014290 43.902 123 60 4 4 117 38 160 4.91e-24 97.8
MS.gene25414.t1 MTR_5g080710 28.066 424 264 15 2 413 59 453 6.97e-24 103
MS.gene25414.t1 MTR_2g086020 27.885 416 197 13 1 414 1 315 5.50e-23 99.0
MS.gene25414.t1 MTR_4g113810 36.471 170 101 5 57 221 1 168 6.12e-22 92.4
MS.gene25414.t1 MTR_4g056040 29.765 383 231 14 27 388 85 450 1.25e-21 97.4
MS.gene25414.t1 MTR_5g084070 27.532 385 237 14 2 376 39 391 6.39e-20 91.7
MS.gene25414.t1 MTR_4g055990 28.342 374 225 14 41 389 106 461 8.96e-20 91.7
MS.gene25414.t1 MTR_4g055990 28.342 374 225 14 41 389 106 461 1.20e-19 91.3
MS.gene25414.t1 MTR_4g056070 29.915 351 196 15 41 364 109 436 2.87e-19 89.7
MS.gene25414.t1 MTR_8g014220 42.975 121 57 5 46 161 47 160 1.37e-17 80.1
MS.gene25414.t1 MTR_4g056030 27.855 359 221 14 41 378 69 410 2.70e-16 80.9
MS.gene25414.t1 MTR_4g056020 28.090 356 226 14 41 379 69 411 2.15e-15 78.2
MS.gene25414.t1 MTR_4g024720 35.821 134 63 6 4 116 45 176 4.90e-15 73.9
MS.gene25414.t1 MTR_4g055580 27.397 365 209 14 44 378 199 537 3.13e-14 75.1
MS.gene25414.t1 MTR_1g038710 26.420 352 228 11 48 378 85 426 5.01e-14 73.9
MS.gene25414.t1 MTR_1g041245 31.361 169 99 4 2 166 37 192 4.53e-13 70.5
MS.gene25414.t1 MTR_1g041245 32.609 138 80 4 286 414 253 386 8.84e-11 63.5
MS.gene25414.t1 MTR_8g014230 48.000 75 35 2 330 404 25 95 3.00e-12 63.2
MS.gene25414.t1 MTR_7g115270 22.353 425 252 14 2 411 38 399 1.45e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25414.t1 AT4G26340 28.103 427 258 16 2 414 28 419 1.40e-25 108
MS.gene25414.t1 AT1G78750 26.450 431 277 15 2 411 44 455 4.75e-22 98.2
MS.gene25414.t1 AT1G78750 26.450 431 277 15 2 411 44 455 4.75e-22 98.2
MS.gene25414.t1 AT1G66300 28.409 440 242 21 3 411 56 453 1.98e-19 90.5
MS.gene25414.t1 AT1G78760 26.959 434 258 21 2 411 42 440 3.61e-19 89.7
MS.gene25414.t1 AT4G13985 26.106 452 254 19 1 411 44 456 3.99e-19 89.4
MS.gene25414.t1 AT5G56370 26.667 435 254 19 2 413 28 420 1.85e-18 87.4
MS.gene25414.t1 AT5G56370 26.667 435 254 19 2 413 28 420 1.85e-18 87.4
MS.gene25414.t1 AT1G66290 27.064 436 252 21 2 411 55 450 2.47e-18 87.0
MS.gene25414.t1 AT1G69630 26.136 440 260 23 2 411 44 448 3.19e-18 86.7
MS.gene25414.t1 AT1G51370 26.897 435 246 23 2 411 45 432 5.72e-18 85.9
MS.gene25414.t1 AT5G56420 24.714 437 259 18 2 414 32 422 7.57e-18 85.5
MS.gene25414.t1 AT5G56420 24.714 437 259 18 2 414 32 422 7.57e-18 85.5
MS.gene25414.t1 AT5G56420 24.714 437 259 18 2 414 32 422 7.57e-18 85.5
MS.gene25414.t1 AT1G32375 25.926 432 262 17 3 414 29 422 4.28e-17 83.2
MS.gene25414.t1 AT1G32375 25.926 432 262 17 3 414 29 422 4.28e-17 83.2
MS.gene25414.t1 AT3G50710 25.404 433 268 17 3 414 29 427 9.22e-17 82.0
MS.gene25414.t1 AT5G22730 27.594 453 242 22 2 411 54 463 9.55e-17 82.4
MS.gene25414.t1 AT5G22730 27.594 453 242 22 2 411 54 463 9.55e-17 82.4
MS.gene25414.t1 AT5G38570 25.891 421 228 16 3 413 29 375 3.93e-16 80.1
MS.gene25414.t1 AT3G54910 26.329 395 247 13 31 414 12 373 5.35e-16 79.3
MS.gene25414.t1 AT3G54910 26.329 395 247 13 31 414 12 373 5.35e-16 79.3
MS.gene25414.t1 AT3G54910 26.329 395 247 13 31 414 12 373 5.35e-16 79.3
MS.gene25414.t1 AT1G06630 28.520 277 173 9 2 266 38 301 1.04e-15 77.8
MS.gene25414.t1 AT1G06630 27.410 332 210 11 2 319 38 352 1.32e-15 78.2
MS.gene25414.t1 AT1G06630 27.410 332 210 11 2 319 38 352 1.32e-15 78.2
MS.gene25414.t1 AT1G06630 27.327 333 211 11 2 320 38 353 1.55e-15 78.2
MS.gene25414.t1 AT1G06630 27.327 333 211 11 2 320 38 353 1.55e-15 78.2
MS.gene25414.t1 AT1G06630 27.327 333 211 11 2 320 38 353 1.55e-15 78.2
MS.gene25414.t1 AT1G06630 27.327 333 211 11 2 320 38 353 1.55e-15 78.2
MS.gene25414.t1 AT1G06630 27.327 333 211 11 2 320 47 362 1.63e-15 78.2
MS.gene25414.t1 AT1G06630 27.327 333 211 11 2 320 38 353 2.10e-15 78.2
MS.gene25414.t1 AT5G60610 25.935 401 234 15 2 380 28 387 2.43e-15 77.8
MS.gene25414.t1 AT5G60610 25.935 401 234 15 2 380 28 387 2.43e-15 77.8
MS.gene25414.t1 AT5G38590 30.417 240 144 10 2 233 28 252 1.10e-14 74.7
MS.gene25414.t1 AT5G53635 24.594 431 273 16 3 413 27 425 1.62e-14 75.5
MS.gene25414.t1 AT3G58920 23.729 472 270 18 2 414 28 468 1.68e-14 75.5
MS.gene25414.t1 AT4G10420 25.723 346 222 13 77 413 2 321 2.33e-14 73.9
MS.gene25414.t1 AT5G22670 29.919 371 215 19 30 376 58 407 2.49e-14 74.7
MS.gene25414.t1 AT1G05080 25.532 423 267 17 3 407 40 432 2.51e-14 74.7
MS.gene25414.t1 AT1G55660 24.249 433 283 17 2 411 79 489 5.92e-14 73.9
MS.gene25414.t1 AT5G56810 27.146 431 253 18 2 411 41 431 7.36e-14 73.6
MS.gene25414.t1 AT5G22720 26.739 460 245 22 2 413 49 464 8.66e-14 73.2
MS.gene25414.t1 AT3G52680 25.172 437 267 18 2 414 47 447 1.34e-13 72.8
MS.gene25414.t1 AT5G56700 26.913 379 230 18 11 375 1 346 1.50e-13 72.0
MS.gene25414.t1 AT2G26030 26.562 448 257 23 2 411 30 443 4.79e-13 70.9
MS.gene25414.t1 AT1G13780 26.619 417 239 20 2 380 36 423 5.88e-13 70.9
MS.gene25414.t1 AT5G56440 24.424 434 274 18 2 413 28 429 7.59e-13 70.1
MS.gene25414.t1 AT5G38590 31.658 199 122 7 41 233 27 217 9.15e-13 68.2
MS.gene25414.t1 AT5G53840 24.528 424 276 17 2 411 44 437 9.65e-13 70.1
MS.gene25414.t1 AT5G44950 27.586 406 238 20 2 380 30 406 1.08e-12 69.7
MS.gene25414.t1 AT2G26860 25.899 417 237 19 2 385 28 405 3.51e-12 68.2
MS.gene25414.t1 AT2G26860 25.899 417 237 19 2 385 28 405 3.51e-12 68.2
MS.gene25414.t1 AT3G49480 26.115 314 194 12 80 375 114 407 4.10e-12 68.2
MS.gene25414.t1 AT5G56390 24.476 429 272 14 3 413 30 424 4.62e-12 67.8
MS.gene25414.t1 AT1G22000 25.197 381 222 15 2 356 55 398 5.43e-12 67.8
MS.gene25414.t1 AT4G00315 24.884 430 284 17 2 411 28 438 6.11e-12 67.4
MS.gene25414.t1 AT1G55660 27.982 218 141 7 2 212 79 287 6.39e-12 67.0
MS.gene25414.t1 AT3G26920 27.954 347 216 15 78 413 16 339 6.57e-12 67.0
MS.gene25414.t1 AT3G26920 27.954 347 216 15 78 413 16 339 6.57e-12 67.0
MS.gene25414.t1 AT3G54160 24.880 418 256 17 24 412 36 424 6.79e-12 67.4
MS.gene25414.t1 AT1G66310 25.455 440 253 22 2 411 45 439 1.11e-11 66.6
MS.gene25414.t1 AT3G49480 26.829 287 176 11 103 375 1 267 1.57e-11 65.5
MS.gene25414.t1 AT5G50270 24.877 406 255 17 3 389 30 404 3.06e-11 65.1
MS.gene25414.t1 AT3G49030 24.594 431 243 17 2 413 47 414 4.12e-11 64.7
MS.gene25414.t1 AT5G56370 27.273 275 167 11 2 269 28 276 4.27e-11 64.3
MS.gene25414.t1 AT4G14096 26.039 361 231 13 2 344 34 376 5.69e-11 64.7
MS.gene25414.t1 AT4G14096 26.039 361 231 13 2 344 34 376 5.69e-11 64.7
MS.gene25414.t1 AT5G56400 24.541 436 262 19 4 414 62 455 6.91e-11 64.3
MS.gene25414.t1 AT4G14103 29.675 246 145 10 2 234 34 264 7.06e-11 63.9
MS.gene25414.t1 AT4G14103 29.675 246 145 10 2 234 34 264 7.06e-11 63.9

Find 85 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGACCTATATCCACTTTAA+AGG 0.185801 8.2:+40441770 MS.gene25414:CDS
ACGTACCAAAAGAGATTATT+AGG 0.292927 8.2:+40441437 MS.gene25414:intron
CCTCTTCTCGTCTGAGGATC+AGG 0.298567 8.2:+40440058 MS.gene25414:CDS
CTTGATGTAGTCTCCTCTTC+TGG 0.309388 8.2:+40441239 MS.gene25414:CDS
CACCACATCAATTTGGTCTT+AGG 0.331226 8.2:+40441708 MS.gene25414:CDS
AAGTGGGATGCAAGACAATT+AGG 0.332413 8.2:-40441749 None:intergenic
CTGAATCTTTGGCCAAGCTT+AGG 0.379532 8.2:+40441057 MS.gene25414:CDS
GGCAGAGAGAAGGATATTAA+TGG 0.390004 8.2:-40440997 None:intergenic
AGCAAAGTCTTTGAAATGTT+CGG 0.401003 8.2:-40439877 None:intergenic
AGTAAATTCAAAACTCTTCA+AGG 0.409402 8.2:-40441090 None:intergenic
TACGACATACCCGCGACATT+CGG 0.412011 8.2:+40439925 MS.gene25414:CDS
ACTCTCCTCCAAGCGCTCTC+TGG 0.418872 8.2:+40441287 MS.gene25414:CDS
TTCGGCCTCGCCGTCGACGC+CGG 0.419368 8.2:-40439859 None:intergenic
TGAATCTTTGGCCAAGCTTA+GGG 0.420723 8.2:+40441058 MS.gene25414:CDS
CGTGGATTCGCACCGCCGTT+GGG 0.424276 8.2:+40440009 MS.gene25414:CDS
GATACAGCACCACATCAATT+TGG 0.425006 8.2:+40441701 MS.gene25414:CDS
AGCCTCACTGATATCACTAT+TGG 0.428235 8.2:+40441170 MS.gene25414:CDS
CAATGAGCCTTGTTTCTCAT+AGG 0.428492 8.2:+40439781 None:intergenic
TCGGCCTCGCCGTCGACGCC+GGG 0.429312 8.2:-40439858 None:intergenic
CTCATAGGTATCTTCATCTC+TGG 0.436906 8.2:+40439796 MS.gene25414:CDS
GGCCAAAGATTCAGTTGAAA+AGG 0.438596 8.2:-40441048 None:intergenic
TGAAGTTATCAAGTTGAGAT+TGG 0.445049 8.2:-40439968 None:intergenic
AGAGGAGGTTAGTGCATGAT+AGG 0.446173 8.2:-40440103 None:intergenic
ATTATAAGAGCTTCAAGTAT+AGG 0.447586 8.2:-40441578 None:intergenic
AGTTTCAAAGTTTCTAAGAC+TGG 0.448465 8.2:-40441023 None:intergenic
ATTTAACTCCAGAGAGCGCT+TGG 0.458373 8.2:-40441295 None:intergenic
TTGGCGATGCCACTTCTGTT+GGG 0.460446 8.2:+40441189 MS.gene25414:CDS
ATCCTCTATATCCTTTAAAG+TGG 0.461419 8.2:-40441781 None:intergenic
ATCCACTTTAAAGGATATAG+AGG 0.462211 8.2:+40441779 MS.gene25414:CDS
TCAAAACTCTTCAAGGAAGA+TGG 0.465651 8.2:-40441083 None:intergenic
GCACTAACCTCCTCTCCATC+AGG 0.467643 8.2:+40440111 MS.gene25414:CDS
CTTGGAGGAGAGTGAGTAAA+GGG 0.475202 8.2:-40441277 None:intergenic
ACGTGGATTCGCACCGCCGT+TGG 0.482549 8.2:+40440008 MS.gene25414:CDS
TTCTACAATGAGTACTCTCC+CGG 0.484115 8.2:+40439840 MS.gene25414:CDS
ATTGGCGATGCCACTTCTGT+TGG 0.491458 8.2:+40441188 MS.gene25414:CDS
TGTAGAATTGCGACAATCAT+CGG 0.493103 8.2:+40440914 MS.gene25414:CDS
ATATCCTTTAAAGTGGATAT+AGG 0.498926 8.2:-40441774 None:intergenic
CATTGTAGAAATGAAAAGCT+TGG 0.503238 8.2:-40439827 None:intergenic
CAAAGTTTCTAAGACTGGAC+AGG 0.505834 8.2:-40441018 None:intergenic
TTAAAGGATATAGAGGATGT+TGG 0.507552 8.2:+40441786 MS.gene25414:CDS
TAGAGGATGTTGGCTTGAGA+TGG 0.521759 8.2:+40441796 MS.gene25414:CDS
AGTTGTAATCTCTTCAATGA+TGG 0.525654 8.2:-40440948 None:intergenic
TAGTGATATCAGTGAGGCTA+AGG 0.528202 8.2:-40441166 None:intergenic
TACAGAAAAGCGTCGAAAGT+AGG 0.534200 8.2:-40441536 None:intergenic
CTCTTCTCGTCTGAGGATCA+GGG 0.539018 8.2:+40440059 MS.gene25414:CDS
ATCCTCAAGGTACGGCCCAA+CGG 0.545220 8.2:-40440024 None:intergenic
CGCCGTTGGGCCGTACCTTG+AGG 0.554002 8.2:+40440022 MS.gene25414:CDS
CCTGATCCTCAGACGAGAAG+AGG 0.554039 8.2:-40440058 None:intergenic
ACGCATCTTCCGAATGTCGC+GGG 0.562648 8.2:-40439934 None:intergenic
TGTAATGCACACAGTCTCTA+CGG 0.564381 8.2:+40440882 MS.gene25414:intron
TCTCGACCTCTTCTCGTCTG+AGG 0.568024 8.2:+40440052 MS.gene25414:CDS
AACAGATTCACTTCCAGAAG+AGG 0.569559 8.2:-40441252 None:intergenic
TAACTCCAGAGAGCGCTTGG+AGG 0.571531 8.2:-40441292 None:intergenic
GCTTGGAGGAGAGTGAGTAA+AGG 0.575040 8.2:-40441278 None:intergenic
TAAAGTGGATATAGGTCAAG+TGG 0.575798 8.2:-40441766 None:intergenic
GGAAGATGGAACCCTAAGCT+TGG 0.579963 8.2:-40441069 None:intergenic
GTGCAAGTTCCCAACAGAAG+TGG 0.580230 8.2:-40441198 None:intergenic
AGACTGGACAGGCAGAGAGA+AGG 0.581233 8.2:-40441007 None:intergenic
CTGAACGCTCTGTTGACACG+TGG 0.585988 8.2:+40439991 MS.gene25414:CDS
GAGTACTCTCCCGGCGTCGA+CGG 0.586422 8.2:+40439849 MS.gene25414:CDS
AGATGAAGATCCTCAAGGTA+CGG 0.599815 8.2:-40440032 None:intergenic
AAGAGATTACAACTAGACAT+TGG 0.609472 8.2:+40440957 MS.gene25414:CDS
CGGAAGATGCGTCTCTCTTG+CGG 0.610368 8.2:+40439945 MS.gene25414:CDS
GAACTTAGTCATATTTAACT+CGG 0.618369 8.2:-40441961 None:intergenic
GGGCTTCAAACTCCCTCTCG+CGG 0.620327 8.2:+40440079 MS.gene25414:CDS
ACAACTAGACATTGGTAATG+TGG 0.620834 8.2:+40440965 MS.gene25414:CDS
AAGGTAACCTGATGGAGAGG+AGG 0.620939 8.2:-40440118 None:intergenic
AGGCTAAGGTACTTGAGACC+AGG 0.622331 8.2:-40441152 None:intergenic
CTCTCCCGGCGTCGACGGCG+AGG 0.630012 8.2:+40439854 MS.gene25414:CDS
CTCAAGGTACGGCCCAACGG+CGG 0.630217 8.2:-40440021 None:intergenic
AAAGTGGATATAGGTCAAGT+GGG 0.643217 8.2:-40441765 None:intergenic
GACGCATCTTCCGAATGTCG+CGG 0.645697 8.2:-40439935 None:intergenic
GTTTAGTTGTAATACAACAA+TGG 0.648312 8.2:+40441325 MS.gene25414:CDS
TGTTGGGAACTTGCACAACG+TGG 0.655593 8.2:+40441205 MS.gene25414:CDS
TGCATGATAGGACCGCGAGA+GGG 0.662917 8.2:-40440091 None:intergenic
GTCCTAAGACCAAATTGATG+TGG 0.669559 8.2:-40441710 None:intergenic
GTGCATGATAGGACCGCGAG+AGG 0.674731 8.2:-40440092 None:intergenic
TGTCTTGAGATAGACACACC+TGG 0.677521 8.2:+40441134 MS.gene25414:CDS
TCTTATAATTCAGAATGACA+AGG 0.677636 8.2:+40441592 MS.gene25414:CDS
GAAGATACCTATGAGAAACA+AGG 0.685034 8.2:-40439788 None:intergenic
GGTCGAGATGAAGATCCTCA+AGG 0.701781 8.2:-40440037 None:intergenic
TGGGAACTTGCACAACGTGG+AGG 0.706891 8.2:+40441208 MS.gene25414:CDS
CGCCAATAGTGATATCAGTG+AGG 0.714173 8.2:-40441172 None:intergenic
CGGCAGACAGTAGATCCCCG+TGG 0.745934 8.2:-40441941 None:intergenic
AAAATTTCGTATATGCCACG+GGG 0.751789 8.2:+40441926 MS.gene25414:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTTTTTTTTTTTTTTGAA+AGG - chr8.2:40440296-40440315 None:intergenic 10.0%
!! TATAAAGGAATATATAATCA+TGG - chr8.2:40441370-40441389 None:intergenic 15.0%
!!! AATGTTTTTTTAGAAAGTTT+AGG - chr8.2:40440482-40440501 None:intergenic 15.0%
!!! TTGTCATATCTTTTATTAAT+TGG - chr8.2:40440620-40440639 None:intergenic 15.0%
!! ACTAAAATACTCAAATATGA+AGG - chr8.2:40440156-40440175 None:intergenic 20.0%
!! ATGAAGGGAAAAAAAAAAAA+AGG - chr8.2:40440140-40440159 None:intergenic 20.0%
!! CTAAAATACTCAAATATGAA+GGG - chr8.2:40440155-40440174 None:intergenic 20.0%
!! GAAGACTACTAAATATATAA+AGG - chr8.2:40441385-40441404 None:intergenic 20.0%
!!! ACGAAATTTTTTTGAGAAAA+TGG - chr8.2:40441916-40441935 None:intergenic 20.0%
!!! TTTTTATTCTACTATTGTAC+TGG + chr8.2:40440729-40440748 MS.gene25414:intron 20.0%
! AAAAAAAAAAGGTAACCTGA+TGG - chr8.2:40440129-40440148 None:intergenic 25.0%
! AAAAAATTTCGTATATGCCA+CGG + chr8.2:40441924-40441943 MS.gene25414:CDS 25.0%
! AGTAAATTCAAAACTCTTCA+AGG - chr8.2:40441093-40441112 None:intergenic 25.0%
! ATTATAAGAGCTTCAAGTAT+AGG - chr8.2:40441581-40441600 None:intergenic 25.0%
! GTTTAGTTGTAATACAACAA+TGG + chr8.2:40441325-40441344 MS.gene25414:CDS 25.0%
! TCTTATAATTCAGAATGACA+AGG + chr8.2:40441592-40441611 MS.gene25414:CDS 25.0%
! TGTTATATGAAAATAACTCG+AGG + chr8.2:40440513-40440532 MS.gene25414:intron 25.0%
!!! ATATCCTTTAAAGTGGATAT+AGG - chr8.2:40441777-40441796 None:intergenic 25.0%
!!! GTGTTGTTTGTTTGATTATT+TGG + chr8.2:40441654-40441673 MS.gene25414:intron 25.0%
!!! TTTTTCGAAAATGCACTTTT+CGG + chr8.2:40441478-40441497 MS.gene25414:CDS 25.0%
AAAAATTTCGTATATGCCAC+GGG + chr8.2:40441925-40441944 MS.gene25414:CDS 30.0%
AAAATAACTCGAGGAAAACT+AGG + chr8.2:40440522-40440541 MS.gene25414:intron 30.0%
AAGAGATTACAACTAGACAT+TGG + chr8.2:40440957-40440976 MS.gene25414:CDS 30.0%
ACGTACCAAAAGAGATTATT+AGG + chr8.2:40441437-40441456 MS.gene25414:intron 30.0%
AGTTGTAATCTCTTCAATGA+TGG - chr8.2:40440951-40440970 None:intergenic 30.0%
CATTGTAGAAATGAAAAGCT+TGG - chr8.2:40439830-40439849 None:intergenic 30.0%
TACAGAGAGTTAGGTATAAT+GGG + chr8.2:40440200-40440219 MS.gene25414:intron 30.0%
TTAAAGGATATAGAGGATGT+TGG + chr8.2:40441786-40441805 MS.gene25414:CDS 30.0%
TTAGAAAGTTTAGGTGAGTT+GGG - chr8.2:40440473-40440492 None:intergenic 30.0%
TTGACCTATATCCACTTTAA+AGG + chr8.2:40441770-40441789 MS.gene25414:CDS 30.0%
TTTAGAAAGTTTAGGTGAGT+TGG - chr8.2:40440474-40440493 None:intergenic 30.0%
! AGACAATTAGGAACACTTTT+CGG - chr8.2:40441740-40441759 None:intergenic 30.0%
! ATCCACTTTAAAGGATATAG+AGG + chr8.2:40441779-40441798 MS.gene25414:CDS 30.0%
! ATCCTCTATATCCTTTAAAG+TGG - chr8.2:40441784-40441803 None:intergenic 30.0%
! GACTACCTAATAATCTCTTT+TGG - chr8.2:40441445-40441464 None:intergenic 30.0%
! GCTAGTTATGTTTTGCATTA+TGG + chr8.2:40441824-40441843 MS.gene25414:CDS 30.0%
! TTCCTTTTCAACTGAATCTT+TGG + chr8.2:40441046-40441065 MS.gene25414:CDS 30.0%
! TTGAAGAAAATGCGTTTTTC+TGG + chr8.2:40441854-40441873 MS.gene25414:CDS 30.0%
! TTTATTTGCTACAGAGAGTT+AGG + chr8.2:40440191-40440210 MS.gene25414:intron 30.0%
!! AGATATGACAAAACTTGCTA+AGG + chr8.2:40440629-40440648 MS.gene25414:intron 30.0%
!! AGCAAAGTCTTTGAAATGTT+CGG - chr8.2:40439880-40439899 None:intergenic 30.0%
!! AGTTTCAAAGTTTCTAAGAC+TGG - chr8.2:40441026-40441045 None:intergenic 30.0%
!! ATTGAGTTTAACTTGAGCTT+TGG + chr8.2:40441622-40441641 MS.gene25414:intron 30.0%
!! TGAAGTTATCAAGTTGAGAT+TGG - chr8.2:40439971-40439990 None:intergenic 30.0%
AAAATTTCGTATATGCCACG+GGG + chr8.2:40441926-40441945 MS.gene25414:CDS 35.0%
AAAGTGGATATAGGTCAAGT+GGG - chr8.2:40441768-40441787 None:intergenic 35.0%
AGAGATTCCTGCATCAATAA+TGG - chr8.2:40440376-40440395 None:intergenic 35.0%
ATCACCAATTACTACTCCAT+CGG - chr8.2:40440550-40440569 None:intergenic 35.0%
CTACAGAGAGTTAGGTATAA+TGG + chr8.2:40440199-40440218 MS.gene25414:intron 35.0%
GAAGATACCTATGAGAAACA+AGG - chr8.2:40439791-40439810 None:intergenic 35.0%
GAGATTCCTGCATCAATAAT+GGG - chr8.2:40440375-40440394 None:intergenic 35.0%
GATCTGTAAGTACGTTTGTT+GGG + chr8.2:40440405-40440424 MS.gene25414:intron 35.0%
TAAAGTGGATATAGGTCAAG+TGG - chr8.2:40441769-40441788 None:intergenic 35.0%
TATTGATGCAGGAATCTCTT+TGG + chr8.2:40440377-40440396 MS.gene25414:intron 35.0%
TCAAAACTCTTCAAGGAAGA+TGG - chr8.2:40441086-40441105 None:intergenic 35.0%
TGATCTGTAAGTACGTTTGT+TGG + chr8.2:40440404-40440423 MS.gene25414:intron 35.0%
TGTAGAATTGCGACAATCAT+CGG + chr8.2:40440914-40440933 MS.gene25414:CDS 35.0%
TTAGACATTCACTTGCAGAA+TGG + chr8.2:40440705-40440724 MS.gene25414:intron 35.0%
!! ACAACTAGACATTGGTAATG+TGG + chr8.2:40440965-40440984 MS.gene25414:CDS 35.0%
!! TTTAACTTGAGCTTTGGTGT+GGG + chr8.2:40441628-40441647 MS.gene25414:intron 35.0%
!!! GACTTTGCTATTTTCGTCAA+CGG + chr8.2:40439891-40439910 MS.gene25414:CDS 35.0%
!!! GCGTTTTTCTGGTTTTTTGT+TGG + chr8.2:40441865-40441884 MS.gene25414:CDS 35.0%
AAAAAGGTAACCTGATGGAG+AGG - chr8.2:40440124-40440143 None:intergenic 40.0%
AACAGATTCACTTCCAGAAG+AGG - chr8.2:40441255-40441274 None:intergenic 40.0%
AAGTGGGATGCAAGACAATT+AGG - chr8.2:40441752-40441771 None:intergenic 40.0%
ACAATGAAAGTTGACTGCGT+TGG + chr8.2:40440313-40440332 MS.gene25414:intron 40.0%
AGATGAAGATCCTCAAGGTA+CGG - chr8.2:40440035-40440054 None:intergenic 40.0%
AGCCTCACTGATATCACTAT+TGG + chr8.2:40441170-40441189 MS.gene25414:CDS 40.0%
CTCATAGGTATCTTCATCTC+TGG + chr8.2:40439796-40439815 MS.gene25414:CDS 40.0%
GATACAGCACCACATCAATT+TGG + chr8.2:40441701-40441720 MS.gene25414:CDS 40.0%
GGCAGAGAGAAGGATATTAA+TGG - chr8.2:40441000-40441019 None:intergenic 40.0%
GGCCAAAGATTCAGTTGAAA+AGG - chr8.2:40441051-40441070 None:intergenic 40.0%
GTCCTAAGACCAAATTGATG+TGG - chr8.2:40441713-40441732 None:intergenic 40.0%
TAGTGATATCAGTGAGGCTA+AGG - chr8.2:40441169-40441188 None:intergenic 40.0%
TCAACTCCCATTATTGATGC+AGG + chr8.2:40440366-40440385 MS.gene25414:intron 40.0%
TGAATCTTTGGCCAAGCTTA+GGG + chr8.2:40441058-40441077 MS.gene25414:CDS 40.0%
TGGTACGTACAAAACACAAG+TGG - chr8.2:40441425-40441444 None:intergenic 40.0%
TGTAATGCACACAGTCTCTA+CGG + chr8.2:40440882-40440901 MS.gene25414:intron 40.0%
TTCTACAATGAGTACTCTCC+CGG + chr8.2:40439840-40439859 MS.gene25414:CDS 40.0%
! CACCACATCAATTTGGTCTT+AGG + chr8.2:40441708-40441727 MS.gene25414:CDS 40.0%
!! CAAAGTTTCTAAGACTGGAC+AGG - chr8.2:40441021-40441040 None:intergenic 40.0%
!! GTTTAACTTGAGCTTTGGTG+TGG + chr8.2:40441627-40441646 MS.gene25414:intron 40.0%
!! TACAGAAAAGCGTCGAAAGT+AGG - chr8.2:40441539-40441558 None:intergenic 40.0%
AGAGGAGGTTAGTGCATGAT+AGG - chr8.2:40440106-40440125 None:intergenic 45.0%
CGCCAATAGTGATATCAGTG+AGG - chr8.2:40441175-40441194 None:intergenic 45.0%
CTGAATCTTTGGCCAAGCTT+AGG + chr8.2:40441057-40441076 MS.gene25414:CDS 45.0%
CTTGATGTAGTCTCCTCTTC+TGG + chr8.2:40441239-40441258 MS.gene25414:CDS 45.0%
CTTGGAGGAGAGTGAGTAAA+GGG - chr8.2:40441280-40441299 None:intergenic 45.0%
GGATCCGATGGAGTAGTAAT+TGG + chr8.2:40440543-40440562 MS.gene25414:intron 45.0%
GTAGAGTTTACTCACCAGCA+AGG + chr8.2:40440335-40440354 MS.gene25414:intron 45.0%
TGTCTTGAGATAGACACACC+TGG + chr8.2:40441134-40441153 MS.gene25414:CDS 45.0%
! AGTTGTACCTTGTTTCCTGC+AGG + chr8.2:40441679-40441698 MS.gene25414:intron 45.0%
!! ATTTAACTCCAGAGAGCGCT+TGG - chr8.2:40441298-40441317 None:intergenic 45.0%
!! TAGAGGATGTTGGCTTGAGA+TGG + chr8.2:40441796-40441815 MS.gene25414:CDS 45.0%
AAGGTAACCTGATGGAGAGG+AGG - chr8.2:40440121-40440140 None:intergenic 50.0%
AGTTGATCTCACCACCTTGC+TGG - chr8.2:40440352-40440371 None:intergenic 50.0%
CGAGGAAAACTAGGATCCGA+TGG + chr8.2:40440531-40440550 MS.gene25414:intron 50.0%
CTCTTCTCGTCTGAGGATCA+GGG + chr8.2:40440059-40440078 MS.gene25414:CDS 50.0%
GAGTTTACTCACCAGCAAGG+TGG + chr8.2:40440338-40440357 MS.gene25414:intron 50.0%
GCTGTATCCTGCAGGAAACA+AGG - chr8.2:40441689-40441708 None:intergenic 50.0%
GCTTGGAGGAGAGTGAGTAA+AGG - chr8.2:40441281-40441300 None:intergenic 50.0%
GGAAGATGGAACCCTAAGCT+TGG - chr8.2:40441072-40441091 None:intergenic 50.0%
GGTCGAGATGAAGATCCTCA+AGG - chr8.2:40440040-40440059 None:intergenic 50.0%
GTGCAAGTTCCCAACAGAAG+TGG - chr8.2:40441201-40441220 None:intergenic 50.0%
TACGACATACCCGCGACATT+CGG + chr8.2:40439925-40439944 MS.gene25414:CDS 50.0%
TGTTGGGAACTTGCACAACG+TGG + chr8.2:40441205-40441224 MS.gene25414:CDS 50.0%
! AGGCTAAGGTACTTGAGACC+AGG - chr8.2:40441155-40441174 None:intergenic 50.0%
! ATTGGCGATGCCACTTCTGT+TGG + chr8.2:40441188-40441207 MS.gene25414:CDS 50.0%
! TTGGCGATGCCACTTCTGTT+GGG + chr8.2:40441189-40441208 MS.gene25414:CDS 50.0%
ACGCATCTTCCGAATGTCGC+GGG - chr8.2:40439937-40439956 None:intergenic 55.0%
AGACTGGACAGGCAGAGAGA+AGG - chr8.2:40441010-40441029 None:intergenic 55.0%
ATCCTCAAGGTACGGCCCAA+CGG - chr8.2:40440027-40440046 None:intergenic 55.0%
CCTCTTCTCGTCTGAGGATC+AGG + chr8.2:40440058-40440077 MS.gene25414:CDS 55.0%
CCTGATCCTCAGACGAGAAG+AGG - chr8.2:40440061-40440080 None:intergenic 55.0%
CGGAAGATGCGTCTCTCTTG+CGG + chr8.2:40439945-40439964 MS.gene25414:CDS 55.0%
CTGAACGCTCTGTTGACACG+TGG + chr8.2:40439991-40440010 MS.gene25414:CDS 55.0%
GACGCATCTTCCGAATGTCG+CGG - chr8.2:40439938-40439957 None:intergenic 55.0%
GCACTAACCTCCTCTCCATC+AGG + chr8.2:40440111-40440130 MS.gene25414:CDS 55.0%
TCTCGACCTCTTCTCGTCTG+AGG + chr8.2:40440052-40440071 MS.gene25414:CDS 55.0%
TGCATGATAGGACCGCGAGA+GGG - chr8.2:40440094-40440113 None:intergenic 55.0%
TGGGAACTTGCACAACGTGG+AGG + chr8.2:40441208-40441227 MS.gene25414:CDS 55.0%
!! GATGTGGTGCTGTATCCTGC+AGG - chr8.2:40441697-40441716 None:intergenic 55.0%
!! TAACTCCAGAGAGCGCTTGG+AGG - chr8.2:40441295-40441314 None:intergenic 55.0%
ACTCTCCTCCAAGCGCTCTC+TGG + chr8.2:40441287-40441306 MS.gene25414:CDS 60.0%
AGAGACCTGCCTTGTCGCTC+GGG + chr8.2:40440436-40440455 MS.gene25414:intron 60.0%
CTAGAAGCTCCCGAGCGACA+AGG - chr8.2:40440448-40440467 None:intergenic 60.0%
GAGAGACCTGCCTTGTCGCT+CGG + chr8.2:40440435-40440454 MS.gene25414:intron 60.0%
GGGCTTCAAACTCCCTCTCG+CGG + chr8.2:40440079-40440098 MS.gene25414:CDS 60.0%
GTGCATGATAGGACCGCGAG+AGG - chr8.2:40440095-40440114 None:intergenic 60.0%
AAGCTCCCGAGCGACAAGGC+AGG - chr8.2:40440444-40440463 None:intergenic 65.0%
ACGTGGATTCGCACCGCCGT+TGG + chr8.2:40440008-40440027 MS.gene25414:CDS 65.0%
CGGCAGACAGTAGATCCCCG+TGG - chr8.2:40441944-40441963 None:intergenic 65.0%
CGTGGATTCGCACCGCCGTT+GGG + chr8.2:40440009-40440028 MS.gene25414:CDS 65.0%
CTCAAGGTACGGCCCAACGG+CGG - chr8.2:40440024-40440043 None:intergenic 65.0%
! GAGTACTCTCCCGGCGTCGA+CGG + chr8.2:40439849-40439868 MS.gene25414:CDS 65.0%
CGCCGTTGGGCCGTACCTTG+AGG + chr8.2:40440022-40440041 MS.gene25414:CDS 70.0%
TTCGGCCTCGCCGTCGACGC+CGG - chr8.2:40439862-40439881 None:intergenic 75.0%
! TCGGCCTCGCCGTCGACGCC+GGG - chr8.2:40439861-40439880 None:intergenic 80.0%
!!! CTCTCCCGGCGTCGACGGCG+AGG + chr8.2:40439854-40439873 MS.gene25414:CDS 80.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 40439783 40441979 40439783 ID=MS.gene25414
chr8.2 mRNA 40439783 40441979 40439783 ID=MS.gene25414.t1;Parent=MS.gene25414
chr8.2 exon 40439783 40440132 40439783 ID=MS.gene25414.t1.exon1;Parent=MS.gene25414.t1
chr8.2 CDS 40439783 40440132 40439783 ID=cds.MS.gene25414.t1;Parent=MS.gene25414.t1
chr8.2 exon 40440896 40441346 40440896 ID=MS.gene25414.t1.exon2;Parent=MS.gene25414.t1
chr8.2 CDS 40440896 40441346 40440896 ID=cds.MS.gene25414.t1;Parent=MS.gene25414.t1
chr8.2 exon 40441449 40441613 40441449 ID=MS.gene25414.t1.exon3;Parent=MS.gene25414.t1
chr8.2 CDS 40441449 40441613 40441449 ID=cds.MS.gene25414.t1;Parent=MS.gene25414.t1
chr8.2 exon 40441701 40441979 40441701 ID=MS.gene25414.t1.exon4;Parent=MS.gene25414.t1
chr8.2 CDS 40441701 40441979 40441701 ID=cds.MS.gene25414.t1;Parent=MS.gene25414.t1
Gene Sequence

>MS.gene25414

ATGAGCCTTGTTTCTCATAGGTATCTTCATCTCTGGAAACATCTCCAAGCTTTTCATTTCTACAATGAGTACTCTCCCGGCGTCGACGGCGAGGCCGAACATTTCAAAGACTTTGCTATTTTCGTCAACGGCTTTCTCTCTTTACGACATACCCGCGACATTCGGAAGATGCGTCTCTCTTGCGGCCAATCTCAACTTGATAACTTCACTGAACGCTCTGTTGACACGTGGATTCGCACCGCCGTTGGGCCGTACCTTGAGGATCTTCATCTCGACCTCTTCTCGTCTGAGGATCAGGGCTTCAAACTCCCTCTCGCGGTCCTATCATGCACTAACCTCCTCTCCATCAGGTTACCTTTTTTTTTTTTTCCCTTCATATTTGAGTATTTTAGTCTCTCATTTTCAATGTTTATTTGCTACAGAGAGTTAGGTATAATGGGATAACAAATTATTAAAATTATTTTATGAAAAAGTAACAAGATTTATTGATTTTAACAATGAAAGTTGACTCCTTTCAAAAAAAAAAAAAAACAATGAAAGTTGACTGCGTTGGTAGAGTTTACTCACCAGCAAGGTGGTGAGATCAACTCCCATTATTGATGCAGGAATCTCTTTGGTAGTTGATCTGTAAGTACGTTTGTTGGGTCTCACTGAGAGACCTGCCTTGTCGCTCGGGAGCTTCTAGAGCCCAACTCACCTAAACTTTCTAAAAAAACATTTATTGATTGTTTGTTATATGAAAATAACTCGAGGAAAACTAGGATCCGATGGAGTAGTAATTGGTGATTTTATGATTATTATGCTAATTAAGCAAAGACATTTTCAATTAACAAACCAATTAATAAAAGATATGACAAAACTTGCTAAGGAAAAGACACTATGTCATGTTTTGTAGCATTGTTACATACATTCAACATTATTCTTAGACATTCACTTGCAGAATGGCTTTTTATTCTACTATTGTACTGGTATTTATTTACAAAAATTACAGTCTTTTTTAGAGCTTCAAAGATATTTTTTTCAGTCATTTCTTTATTTATTATTTGCACACTTGTTCTTGTTAAATGTGACATGATAAATGATGATAACATAAGATTTGTGTAATGCACACAGTCTCTACGGTGAGATCTTTGTAGAATTGCGACAATCATCGGCTGTTTGTTTACCATCATTGAAGAGATTACAACTAGACATTGGTAATGTGGATGTGAATGCCATTAATATCCTTCTCTCTGCCTGTCCAGTCTTAGAAACTTTGAAACTTTCCTTTTCAACTGAATCTTTGGCCAAGCTTAGGGTTCCATCTTCCTTGAAGAGTTTTGAATTTACTGTAGAGAATGAAATTGAAGTTTGTCTTGAGATAGACACACCTGGTCTCAAGTACCTTAGCCTCACTGATATCACTATTGGCGATGCCACTTCTGTTGGGAACTTGCACAACGTGGAGGAAGCATATCTTGATGTAGTCTCCTCTTCTGGAAGTGAATCTGTTGACCCTTTACTCACTCTCCTCCAAGCGCTCTCTGGAGTTAAATGTCTAGTGTTTAGTTGTAATACAACAATGGTGAGTGCAATTTTCGTTATCCATGATTATATATTCCTTTATATATTTAGTAGTCTTCTGCTAATTCTTGTATCTCCACTTGTGTTTTGTACGTACCAAAAGAGATTATTAGGTAGTCAACGTATTATAGATTTTTCGAAAATGCACTTTTCGGAATTTCGCTGTTTACTACATCTAGAGCTCATTCTTCCTACTTTCGACGCTTTTCTGTACGTCGTGCTTCAGAAATGTCCTATACTTGAAGCTCTTATAATTCAGAATGACAAGGTTCGTGAATTGAGTTTAACTTGAGCTTTGGTGTGGGTGTGTGTTGTTTGTTTGATTATTTGGTGAGTTGTACCTTGTTTCCTGCAGGATACAGCACCACATCAATTTGGTCTTAGGACAAAGCCGAAAAGTGTTCCTAATTGTCTTGCATCCCACTTGACCTATATCCACTTTAAAGGATATAGAGGATGTTGGCTTGAGATGGACTTTGCTAGTTATGTTTTGCATTATGGACTTGTTTTGAAGAAAATGCGTTTTTCTGGTTTTTTGTTGGATGAAAGAAAGAAGTCGACAAAGTACCATTTTCTCAAAAAAATTTCGTATATGCCACGGGGATCTACTGTCTGCCGAGTTAAATATGACTAA
Protein sequence

>MS.gene25414.t1

MSLVSHRYLHLWKHLQAFHFYNEYSPGVDGEAEHFKDFAIFVNGFLSLRHTRDIRKMRLSCGQSQLDNFTERSVDTWIRTAVGPYLEDLHLDLFSSEDQGFKLPLAVLSCTNLLSISLYGEIFVELRQSSAVCLPSLKRLQLDIGNVDVNAINILLSACPVLETLKLSFSTESLAKLRVPSSLKSFEFTVENEIEVCLEIDTPGLKYLSLTDITIGDATSVGNLHNVEEAYLDVVSSSGSESVDPLLTLLQALSGVKCLVFSCNTTMRLLGSQRIIDFSKMHFSEFRCLLHLELILPTFDAFLYVVLQKCPILEALIIQNDKDTAPHQFGLRTKPKSVPNCLASHLTYIHFKGYRGCWLEMDFASYVLHYGLVLKKMRFSGFLLDERKKSTKYHFLKKISYMPRGSTVCRVKYD