AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39744


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39744.t1 MTR_7g056433 56.805 169 72 1 8 175 129 297 8.14e-52 170
MS.gene39744.t1 MTR_7g115270 48.023 177 82 3 9 175 134 310 5.31e-39 137
MS.gene39744.t1 MTR_7g056417 47.682 151 71 2 8 150 4 154 1.28e-32 114
MS.gene39744.t1 MTR_7g056447 72.500 80 21 1 5 83 119 198 7.26e-31 113
MS.gene39744.t1 MTR_5g013500 45.860 157 73 4 31 175 116 272 3.14e-30 113
MS.gene39744.t1 MTR_7g098510 42.308 182 91 5 1 175 125 299 1.33e-28 109
MS.gene39744.t1 MTR_7g098570 43.275 171 89 3 7 173 133 299 1.64e-28 110
MS.gene39744.t1 MTR_7g056430 41.011 178 73 4 5 175 118 270 8.53e-27 106
MS.gene39744.t1 MTR_7g056420 41.011 178 73 4 5 175 118 270 8.74e-27 106
MS.gene39744.t1 MTR_3g036250 42.458 179 74 6 6 175 119 277 1.28e-25 100
MS.gene39744.t1 MTR_7g098480 40.476 168 92 4 9 173 133 295 9.29e-24 97.1
MS.gene39744.t1 MTR_3g040700 40.000 175 80 6 8 175 159 315 2.21e-22 92.8
MS.gene39744.t1 MTR_7g034120 40.964 166 89 6 14 176 64 223 2.30e-22 91.7
MS.gene39744.t1 MTR_1g041245 40.341 176 92 8 6 175 124 292 2.45e-22 92.4
MS.gene39744.t1 MTR_3g023630 39.326 178 95 7 1 175 119 286 3.45e-22 92.0
MS.gene39744.t1 MTR_7g033990 40.580 138 77 3 39 176 162 294 2.85e-21 89.7
MS.gene39744.t1 MTR_5g008950 35.465 172 97 6 9 175 111 273 3.90e-21 89.0
MS.gene39744.t1 MTR_2g007450 37.097 186 90 5 10 175 151 329 9.59e-20 85.5
MS.gene39744.t1 MTR_7g033915 43.478 115 62 2 62 176 165 276 1.22e-19 84.7
MS.gene39744.t1 MTR_3g107100 36.416 173 97 6 10 176 131 296 3.26e-19 84.0
MS.gene39744.t1 MTR_7g034115 34.831 178 104 6 3 175 121 291 4.77e-19 83.2
MS.gene39744.t1 MTR_7g007080 35.632 174 91 6 11 176 129 289 5.13e-19 83.2
MS.gene39744.t1 MTR_6g011460 37.791 172 91 6 11 175 166 328 9.24e-19 82.8
MS.gene39744.t1 MTR_5g007410 35.260 173 104 3 11 175 145 317 2.43e-18 81.6
MS.gene39744.t1 MTR_1g107385 39.326 178 82 9 10 175 165 328 3.94e-18 80.9
MS.gene39744.t1 MTR_6g045333 37.288 177 95 4 8 173 180 351 7.13e-18 80.5
MS.gene39744.t1 MTR_0009s0080 36.723 177 94 5 11 175 138 308 1.41e-17 79.3
MS.gene39744.t1 MTR_3g071850 32.955 176 104 2 8 173 143 314 3.92e-17 78.6
MS.gene39744.t1 MTR_2g007400 33.880 183 104 7 1 176 134 306 4.84e-17 77.8
MS.gene39744.t1 MTR_5g083890 37.500 176 89 7 10 175 141 305 2.58e-16 75.9
MS.gene39744.t1 MTR_3g108560 36.905 168 96 5 15 176 135 298 3.49e-16 74.7
MS.gene39744.t1 MTR_5g073370 37.870 169 97 6 9 175 126 288 3.63e-16 75.1
MS.gene39744.t1 MTR_1g053025 29.609 179 106 4 10 175 128 299 5.87e-16 74.7
MS.gene39744.t1 MTR_3g108560 36.905 168 96 5 15 176 135 298 6.35e-16 74.7
MS.gene39744.t1 MTR_5g073420 34.146 164 94 5 14 175 137 288 6.36e-16 74.7
MS.gene39744.t1 MTR_0492s0020 36.000 175 97 6 7 176 195 359 1.78e-15 73.6
MS.gene39744.t1 MTR_0543s0010 36.000 175 97 6 7 176 195 359 2.45e-15 73.2
MS.gene39744.t1 MTR_3g105060 32.738 168 101 4 9 174 131 288 2.52e-15 72.8
MS.gene39744.t1 MTR_7g056277 36.905 168 90 6 6 169 376 531 3.13e-15 72.8
MS.gene39744.t1 MTR_1g085120 33.721 172 99 5 15 176 303 469 3.80e-15 72.4
MS.gene39744.t1 MTR_3g087260 36.872 179 89 8 8 175 198 363 5.05e-15 72.0
MS.gene39744.t1 MTR_5g039470 34.426 183 101 5 5 173 95 272 5.40e-15 71.6
MS.gene39744.t1 MTR_7g075820 34.682 173 99 6 10 176 141 305 5.57e-15 72.0
MS.gene39744.t1 MTR_4g039440 30.270 185 103 5 7 175 145 319 5.60e-15 72.0
MS.gene39744.t1 MTR_7g056287 36.364 165 89 6 9 169 123 275 6.24e-15 71.6
MS.gene39744.t1 MTR_5g045170 34.444 180 101 5 6 175 128 300 6.26e-15 71.6
MS.gene39744.t1 MTR_1g085120 32.759 174 96 5 15 175 216 381 1.06e-14 71.2
MS.gene39744.t1 MTR_1g085120 32.759 174 96 5 15 175 291 456 1.20e-14 71.2
MS.gene39744.t1 MTR_2g007500 34.409 186 97 7 6 175 116 292 1.48e-14 70.5
MS.gene39744.t1 MTR_5g073340 33.908 174 95 6 14 175 105 270 3.19e-14 69.3
MS.gene39744.t1 MTR_5g058000 33.728 169 103 5 9 175 124 285 3.40e-14 69.7
MS.gene39744.t1 MTR_5g061410 34.524 168 102 4 14 176 104 268 4.50e-14 68.6
MS.gene39744.t1 MTR_7g088260 34.463 177 97 6 8 175 205 371 2.11e-13 67.4
MS.gene39744.t1 MTR_6g022500 31.953 169 100 5 15 175 225 386 3.42e-13 67.0
MS.gene39744.t1 MTR_5g008920 32.544 169 107 5 7 173 125 288 3.81e-13 66.6
MS.gene39744.t1 MTR_7g060470 33.702 181 98 7 12 175 126 301 5.80e-13 66.2
MS.gene39744.t1 MTR_1g108820 30.387 181 101 7 14 176 126 299 7.35e-13 65.9
MS.gene39744.t1 MTR_1g108800 33.742 163 89 6 14 166 93 246 7.60e-13 65.5
MS.gene39744.t1 MTR_2g008320 33.143 175 97 6 11 175 173 337 7.75e-13 65.9
MS.gene39744.t1 MTR_3g099830 33.136 169 100 7 10 175 117 275 1.03e-12 65.5
MS.gene39744.t1 MTR_3g026920 31.183 186 86 8 6 174 159 319 1.26e-12 65.1
MS.gene39744.t1 MTR_3g107120 34.503 171 101 5 9 175 140 303 1.80e-12 64.7
MS.gene39744.t1 MTR_3g082890 31.915 188 105 6 9 176 130 314 2.24e-12 64.7
MS.gene39744.t1 MTR_6g040270 32.544 169 104 6 11 175 127 289 2.39e-12 64.3
MS.gene39744.t1 MTR_1g108820 30.220 182 100 8 14 176 126 299 2.50e-12 64.3
MS.gene39744.t1 MTR_7g032370 33.918 171 89 7 11 175 108 260 2.75e-12 63.9
MS.gene39744.t1 MTR_1089s0010 31.646 158 100 4 14 169 130 281 3.31e-12 63.9
MS.gene39744.t1 MTR_7g007340 34.118 170 94 6 10 175 107 262 3.66e-12 63.9
MS.gene39744.t1 MTR_5g063950 29.834 181 90 2 14 176 107 268 4.41e-12 63.5
MS.gene39744.t1 MTR_3g082890 31.551 187 105 6 9 175 210 393 5.17e-12 63.5
MS.gene39744.t1 MTR_2g036470 34.270 178 105 4 7 176 131 304 1.04e-11 62.4
MS.gene39744.t1 MTR_1g066830 33.333 168 94 5 14 169 130 291 1.05e-11 62.4
MS.gene39744.t1 MTR_3g109982 34.483 174 97 7 11 176 220 384 1.47e-11 62.4
MS.gene39744.t1 MTR_7g026500 33.146 178 96 9 9 175 140 305 2.04e-11 61.6
MS.gene39744.t1 MTR_3g110010 34.682 173 96 7 11 175 220 383 2.05e-11 62.0
MS.gene39744.t1 MTR_6g022360 33.526 173 101 6 11 175 150 316 4.14e-11 60.8
MS.gene39744.t1 MTR_5g045510 32.895 152 92 3 11 155 129 277 4.84e-11 60.5
MS.gene39744.t1 MTR_3g108150 35.429 175 101 5 9 176 126 295 5.53e-11 60.5
MS.gene39744.t1 MTR_3g467400 42.157 102 55 3 5 103 128 228 5.96e-11 60.5
MS.gene39744.t1 MTR_7g114710 32.278 158 77 6 20 175 142 271 8.21e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39744.t1 AT2G26860 30.374 214 95 8 9 173 122 330 2.65e-11 61.6
MS.gene39744.t1 AT2G26860 30.374 214 95 8 9 173 122 330 2.65e-11 61.6

Find 28 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGTTGTTCTTCGTTTGTA+TGG 0.252657 7.1:+45456501 MS.gene39744:CDS
TCCTCTAAAACAAGTCTCTT+TGG 0.260876 7.1:-45456687 None:intergenic
TATCTTCCAAAGCTTCTATT+AGG 0.306353 7.1:+45456615 MS.gene39744:CDS
ATGTTTGCTTTGATCAAATT+CGG 0.346846 7.1:-45456726 None:intergenic
AATTTGAGTCTAAAGCTAAC+AGG 0.382691 7.1:-45456450 None:intergenic
TACATAATTTCTGTGAGAAC+TGG 0.384165 7.1:+45456967 MS.gene39744:CDS
TATTAGCAGTTCCTCTAGAA+TGG 0.388011 7.1:-45456643 None:intergenic
AAAGTTTCTGTGTGTAGAAC+TGG 0.397697 7.1:+45456800 MS.gene39744:CDS
AACCTCAATCTCCGAATGTC+TGG 0.406460 7.1:+45456426 None:intergenic
GGGCATCCTAATAGAAGCTT+TGG 0.407135 7.1:-45456621 None:intergenic
TCAAAATCTCACCATTCATC+AGG 0.411739 7.1:+45457028 MS.gene39744:CDS
ATTAGCAGTTCCTCTAGAAT+GGG 0.415017 7.1:-45456642 None:intergenic
TGACCTTTCTCGTGTGGTTG+TGG 0.449425 7.1:+45456536 MS.gene39744:CDS
TTGTTGTTCTTCGTTTGTAT+GGG 0.471921 7.1:+45456502 MS.gene39744:CDS
TGTAGGGAAATAGCTGTAAC+CGG 0.475182 7.1:-45456910 None:intergenic
GCTGTTTACTTTGCTTTGCA+AGG 0.528075 7.1:+45456776 MS.gene39744:CDS
AATCCACAACCACACGAGAA+AGG 0.549947 7.1:-45456539 None:intergenic
ATTTCAAAATCTAATCCACT+TGG 0.551657 7.1:+45456932 MS.gene39744:CDS
AATCGACTAAACCTGATGAA+TGG 0.563238 7.1:-45457039 None:intergenic
TGTAAATCACAACAGGAGAC+CGG 0.590280 7.1:+45456891 MS.gene39744:intron
TTCTGAGTTGTGTTCTAGTG+CGG 0.593981 7.1:+45456749 MS.gene39744:CDS
ATTAGGATGCCCCATTCTAG+AGG 0.601001 7.1:+45456632 MS.gene39744:CDS
TTAGCAGTTCCTCTAGAATG+GGG 0.611891 7.1:-45456641 None:intergenic
CATACAAACGAAGAACAACA+AGG 0.641315 7.1:-45456500 None:intergenic
GTATAAGAATAAGTTCCAAG+TGG 0.643484 7.1:-45456947 None:intergenic
AGTGAATGACCTTTCTCGTG+TGG 0.647999 7.1:+45456530 MS.gene39744:CDS
ATTTGAGTCTAAAGCTAACA+GGG 0.666369 7.1:-45456449 None:intergenic
ATACAAACGAAGAACAACAA+GGG 0.729811 7.1:-45456499 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTCTTTTAAACATATAA+CGG - chr7.1:45456848-45456867 None:intergenic 10.0%
!!! GCTAATAAACTTTGTATTTT+TGG + chr7.1:45456659-45456678 MS.gene39744:CDS 20.0%
! ATGTTTGCTTTGATCAAATT+CGG - chr7.1:45456729-45456748 None:intergenic 25.0%
! ATTTCAAAATCTAATCCACT+TGG + chr7.1:45456932-45456951 MS.gene39744:CDS 25.0%
!!! GGATTAGATTTTGAAATGTA+GGG - chr7.1:45456929-45456948 None:intergenic 25.0%
!!! TGGATTAGATTTTGAAATGT+AGG - chr7.1:45456930-45456949 None:intergenic 25.0%
ATACAAACGAAGAACAACAA+GGG - chr7.1:45456502-45456521 None:intergenic 30.0%
GTATAAGAATAAGTTCCAAG+TGG - chr7.1:45456950-45456969 None:intergenic 30.0%
TAACTTCTGTAAATCACAAC+AGG + chr7.1:45456884-45456903 MS.gene39744:intron 30.0%
TACATAATTTCTGTGAGAAC+TGG + chr7.1:45456967-45456986 MS.gene39744:CDS 30.0%
TATCTTCCAAAGCTTCTATT+AGG + chr7.1:45456615-45456634 MS.gene39744:CDS 30.0%
! AATTTGAGTCTAAAGCTAAC+AGG - chr7.1:45456453-45456472 None:intergenic 30.0%
! ATTTGAGTCTAAAGCTAACA+GGG - chr7.1:45456452-45456471 None:intergenic 30.0%
! TTGTTGTTCTTCGTTTGTAT+GGG + chr7.1:45456502-45456521 MS.gene39744:CDS 30.0%
!!! AAATCAAGAGTTTTGAGACA+TGG - chr7.1:45456567-45456586 None:intergenic 30.0%
!!! AATCAAGAGTTTTGAGACAT+GGG - chr7.1:45456566-45456585 None:intergenic 30.0%
!!! AATGGTGAGATTTTGAAGTT+TGG - chr7.1:45457024-45457043 None:intergenic 30.0%
!!! ATGGTGAGATTTTGAAGTTT+GGG - chr7.1:45457023-45457042 None:intergenic 30.0%
AAAGTTTCTGTGTGTAGAAC+TGG + chr7.1:45456800-45456819 MS.gene39744:CDS 35.0%
CATACAAACGAAGAACAACA+AGG - chr7.1:45456503-45456522 None:intergenic 35.0%
TCAAAATCTCACCATTCATC+AGG + chr7.1:45457028-45457047 MS.gene39744:CDS 35.0%
! TATTAGCAGTTCCTCTAGAA+TGG - chr7.1:45456646-45456665 None:intergenic 35.0%
!! ATTAGCAGTTCCTCTAGAAT+GGG - chr7.1:45456645-45456664 None:intergenic 35.0%
!! CTTGTTGTTCTTCGTTTGTA+TGG + chr7.1:45456501-45456520 MS.gene39744:CDS 35.0%
!! TCCTCTAAAACAAGTCTCTT+TGG - chr7.1:45456690-45456709 None:intergenic 35.0%
!!! TCCAAAGAGACTTGTTTTAG+AGG + chr7.1:45456686-45456705 MS.gene39744:CDS 35.0%
GCTGTTTACTTTGCTTTGCA+AGG + chr7.1:45456776-45456795 MS.gene39744:CDS 40.0%
TCACAGCTAAAAATGCTACG+AGG - chr7.1:45456477-45456496 None:intergenic 40.0%
TGTAAATCACAACAGGAGAC+CGG + chr7.1:45456891-45456910 MS.gene39744:intron 40.0%
TGTAGGGAAATAGCTGTAAC+CGG - chr7.1:45456913-45456932 None:intergenic 40.0%
TTCTGAGTTGTGTTCTAGTG+CGG + chr7.1:45456749-45456768 MS.gene39744:CDS 40.0%
!! TTAGCAGTTCCTCTAGAATG+GGG - chr7.1:45456644-45456663 None:intergenic 40.0%
AATCCACAACCACACGAGAA+AGG - chr7.1:45456542-45456561 None:intergenic 45.0%
AGTGAATGACCTTTCTCGTG+TGG + chr7.1:45456530-45456549 MS.gene39744:CDS 45.0%
ATTAGGATGCCCCATTCTAG+AGG + chr7.1:45456632-45456651 MS.gene39744:CDS 45.0%
GGGCATCCTAATAGAAGCTT+TGG - chr7.1:45456624-45456643 None:intergenic 45.0%
TGACCTTTCTCGTGTGGTTG+TGG + chr7.1:45456536-45456555 MS.gene39744:CDS 50.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 45456441 45457055 45456441 ID=MS.gene39744
chr7.1 mRNA 45456441 45457055 45456441 ID=MS.gene39744.t1;Parent=MS.gene39744
chr7.1 exon 45456441 45456821 45456441 ID=MS.gene39744.t1.exon1;Parent=MS.gene39744.t1
chr7.1 CDS 45456441 45456821 45456441 ID=cds.MS.gene39744.t1;Parent=MS.gene39744.t1
chr7.1 exon 45456906 45457055 45456906 ID=MS.gene39744.t1.exon2;Parent=MS.gene39744.t1
chr7.1 CDS 45456906 45457055 45456906 ID=cds.MS.gene39744.t1;Parent=MS.gene39744.t1
Gene Sequence

>MS.gene39744

ATGTCTGGCCCTGTTAGCTTTAGACTCAAATTACCTCGTAGCATTTTTAGCTGTGACACCCTTGTTGTTCTTCGTTTGTATGGGATATCAGTGAATGACCTTTCTCGTGTGGTTGTGGATTTCCCATGTCTCAAAACTCTTGATTTGTCTCGTGTATTTTTCAAACGTGTTGAATATCTTCCAAAGCTTCTATTAGGATGCCCCATTCTAGAGGAACTGCTAATAAACTTTGTATTTTTGGTTAATCCAAAGAGACTTGTTTTAGAGGAAAATTATCAATGCTTACCGAATTTGATCAAAGCAAACATTTCTGAGTTGTGTTCTAGTGCGGATGCGCTGTTTACTTTGCTTTGCAAGGCAAAGTTTCTGTGTGTAGAACTGGTAAGAATATTATACGACTCTCTCCGTTATATGTTTAAAAGAAATTACGTTTATTTCATTTCTAACTTCTGTAAATCACAACAGGAGACCGGTTACAGCTATTTCCCTACATTTCAAAATCTAATCCACTTGGAACTTATTCTTATACATAATTTCTGTGAGAACTGGAAGTGTGTTATAGAAGTGCTGAAACTTAGTCCCAAACTTCAAAATCTCACCATTCATCAGGTTTAG

Protein sequence

>MS.gene39744.t1

MSGPVSFRLKLPRSIFSCDTLVVLRLYGISVNDLSRVVVDFPCLKTLDLSRVFFKRVEYLPKLLLGCPILEELLINFVFLVNPKRLVLEENYQCLPNLIKANISELCSSADALFTLLCKAKFLCVELETGYSYFPTFQNLIHLELILIHNFCENWKCVIEVLKLSPKLQNLTIHQV