AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43257

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43257.t1 MTR_3g082890 70.979 286 60 4 1 267 203 484 5.38e-134 387
MS.gene43257.t1 MTR_3g082890 62.539 323 61 5 1 267 123 441 4.22e-127 368
MS.gene43257.t1 MTR_2g020180 67.841 227 45 4 56 267 2 215 8.58e-92 270
MS.gene43257.t1 MTR_7g081290 76.923 156 28 3 114 267 107 256 1.04e-78 238
MS.gene43257.t1 MTR_7g098510 42.910 268 136 9 8 267 133 391 1.45e-60 196
MS.gene43257.t1 MTR_3g113770 50.673 223 43 5 9 227 175 334 1.92e-57 187
MS.gene43257.t1 MTR_7g032370 42.471 259 126 9 14 267 112 352 2.16e-50 169
MS.gene43257.t1 MTR_7g098570 39.695 262 142 7 6 261 133 384 3.71e-49 169
MS.gene43257.t1 MTR_1g041245 42.336 274 144 9 1 267 120 386 5.86e-49 166
MS.gene43257.t1 MTR_7g056433 37.545 277 139 8 8 267 130 389 1.05e-48 166
MS.gene43257.t1 MTR_7g098480 40.310 258 140 7 8 261 133 380 1.07e-47 165
MS.gene43257.t1 MTR_3g115430 59.231 130 47 2 139 267 1 125 3.85e-47 153
MS.gene43257.t1 MTR_3g036250 39.850 266 125 10 9 264 123 363 2.07e-46 159
MS.gene43257.t1 MTR_0543s0010 38.202 267 147 8 6 266 195 449 1.81e-45 158
MS.gene43257.t1 MTR_3g087260 41.481 270 143 8 4 266 195 456 1.91e-44 156
MS.gene43257.t1 MTR_3g113785 81.522 92 14 1 139 227 1 92 8.94e-44 144
MS.gene43257.t1 MTR_5g008950 36.704 267 150 8 8 267 111 365 3.08e-43 151
MS.gene43257.t1 MTR_0492s0020 38.039 255 143 7 6 257 195 437 8.60e-43 151
MS.gene43257.t1 MTR_7g115270 37.545 277 142 9 8 264 134 399 1.04e-42 150
MS.gene43257.t1 MTR_7g033915 39.130 276 119 12 4 266 125 364 5.57e-42 147
MS.gene43257.t1 MTR_7g034115 38.376 271 143 8 6 267 125 380 7.67e-42 147
MS.gene43257.t1 MTR_7g088260 37.918 269 146 10 8 267 206 462 1.08e-41 149
MS.gene43257.t1 MTR_7g033990 41.949 236 111 9 33 261 158 374 1.54e-41 147
MS.gene43257.t1 MTR_7g034120 39.502 281 129 14 4 267 52 308 2.85e-41 144
MS.gene43257.t1 MTR_7g032380 36.330 267 123 9 9 267 129 356 2.54e-40 143
MS.gene43257.t1 MTR_3g108150 38.290 269 148 8 8 266 126 386 4.19e-39 140
MS.gene43257.t1 MTR_3g040700 38.158 228 114 5 9 230 161 367 1.11e-38 140
MS.gene43257.t1 MTR_5g013500 38.911 257 130 9 31 267 116 365 2.21e-38 138
MS.gene43257.t1 MTR_5g008920 35.206 267 158 7 6 267 125 381 2.70e-36 133
MS.gene43257.t1 MTR_3g109982 41.016 256 137 9 10 260 220 466 2.74e-36 137
MS.gene43257.t1 MTR_3g108560 36.015 261 151 8 5 256 122 375 2.97e-36 133
MS.gene43257.t1 MTR_1g043170 67.949 78 25 0 190 267 11 88 5.49e-36 124
MS.gene43257.t1 MTR_7g056020 36.680 259 146 8 9 261 119 365 1.38e-34 131
MS.gene43257.t1 MTR_7g056420 37.931 261 129 10 8 261 122 356 9.55e-34 129
MS.gene43257.t1 MTR_7g056430 37.931 261 129 10 8 261 122 356 1.16e-33 129
MS.gene43257.t1 MTR_5g012840 35.606 264 113 11 10 267 106 318 4.35e-33 123
MS.gene43257.t1 MTR_3g467400 38.519 270 124 11 7 267 131 367 1.36e-32 122
MS.gene43257.t1 MTR_4g080610 37.402 254 131 12 28 266 116 356 1.86e-32 122
MS.gene43257.t1 MTR_3g023630 32.283 254 154 7 10 261 128 365 1.58e-31 120
MS.gene43257.t1 MTR_3g105060 32.714 269 146 11 8 267 131 373 1.15e-28 112
MS.gene43257.t1 MTR_8g085430 53.333 105 46 3 156 258 213 316 3.21e-27 107
MS.gene43257.t1 MTR_7g026500 32.130 277 148 12 8 266 140 394 5.90e-27 108
MS.gene43257.t1 MTR_7g114710 35.659 258 124 11 18 267 141 364 8.86e-27 107
MS.gene43257.t1 MTR_7g007080 34.191 272 146 10 7 265 126 377 2.30e-26 106
MS.gene43257.t1 MTR_7g056277 33.992 253 144 8 8 255 379 613 1.24e-25 105
MS.gene43257.t1 MTR_4g098590 30.888 259 168 6 3 255 120 373 3.11e-25 103
MS.gene43257.t1 MTR_6g022500 32.157 255 139 10 14 257 225 456 3.20e-24 101
MS.gene43257.t1 MTR_3g107120 30.682 264 152 9 7 260 139 381 3.55e-24 100
MS.gene43257.t1 MTR_7g056287 33.202 253 146 8 8 255 123 357 4.62e-24 99.8
MS.gene43257.t1 MTR_1g053025 27.437 277 164 10 9 265 128 387 1.79e-23 98.6
MS.gene43257.t1 MTR_2g007450 30.952 252 141 6 9 237 151 392 5.65e-23 97.4
MS.gene43257.t1 MTR_3g107100 30.522 249 144 7 9 244 131 363 9.26e-23 96.7
MS.gene43257.t1 MTR_1g108820 29.537 281 156 10 8 264 121 383 1.40e-22 95.9
MS.gene43257.t1 MTR_5g058000 32.117 274 150 10 7 266 123 374 8.39e-22 93.6
MS.gene43257.t1 MTR_4g039440 29.433 282 165 8 6 267 145 412 8.94e-22 94.0
MS.gene43257.t1 MTR_1g066830 29.391 279 156 11 10 266 127 386 1.75e-21 92.8
MS.gene43257.t1 MTR_5g058100 31.868 273 147 10 14 267 132 384 2.03e-21 92.8
MS.gene43257.t1 MTR_6g040270 29.304 273 154 10 10 264 127 378 2.67e-21 92.4
MS.gene43257.t1 MTR_6g045333 30.657 274 166 8 8 266 181 445 4.65e-21 92.0
MS.gene43257.t1 MTR_1g108800 31.955 266 148 8 13 264 93 339 4.83e-21 91.3
MS.gene43257.t1 MTR_1g085120 28.625 269 164 9 14 267 216 471 1.67e-20 90.9
MS.gene43257.t1 MTR_1g085120 28.625 269 164 9 14 267 291 546 1.80e-20 90.9
MS.gene43257.t1 MTR_5g054920 34.973 183 96 6 68 240 11 180 2.46e-20 86.3
MS.gene43257.t1 MTR_1089s0010 29.032 279 167 11 3 267 118 379 5.79e-20 88.6
MS.gene43257.t1 MTR_7g055943 28.622 283 156 9 10 267 160 421 9.72e-20 88.2
MS.gene43257.t1 MTR_7g021990 30.952 252 161 6 14 258 168 413 1.32e-19 87.8
MS.gene43257.t1 MTR_6g022520 30.389 283 160 10 3 267 50 313 1.37e-19 86.7
MS.gene43257.t1 MTR_7g021950 30.952 252 161 6 14 258 168 413 1.61e-19 88.2
MS.gene43257.t1 MTR_3g107940 39.695 131 62 3 132 260 88 203 1.69e-19 87.0
MS.gene43257.t1 MTR_5g063950 29.368 269 167 8 11 267 105 362 2.77e-19 86.7
MS.gene43257.t1 MTR_0009s0080 30.712 267 147 10 10 256 138 386 2.88e-19 87.0
MS.gene43257.t1 MTR_7g060470 30.859 256 134 10 14 244 129 366 3.53e-19 86.7
MS.gene43257.t1 MTR_6g011460 29.885 261 157 9 1 256 161 400 4.43e-19 86.3
MS.gene43257.t1 MTR_5g008910 54.878 82 29 4 190 267 225 302 5.19e-19 85.1
MS.gene43257.t1 MTR_5g073370 31.250 240 137 10 8 237 126 347 1.10e-18 85.1
MS.gene43257.t1 MTR_3g071850 29.151 271 159 10 8 260 144 399 1.18e-18 85.9
MS.gene43257.t1 MTR_7g022105 30.078 256 152 7 14 255 169 411 1.48e-18 84.7
MS.gene43257.t1 MTR_5g007410 28.058 278 167 10 1 260 136 398 1.51e-18 85.1
MS.gene43257.t1 MTR_5g045170 28.519 270 159 8 2 255 125 376 1.85e-18 84.3
MS.gene43257.t1 MTR_5g073420 27.083 240 144 8 10 242 134 349 2.16e-18 84.3
MS.gene43257.t1 MTR_2g007500 29.596 223 130 5 9 214 120 332 2.57e-18 83.6
MS.gene43257.t1 MTR_6g022360 30.389 283 156 11 3 266 147 407 3.65e-18 83.6
MS.gene43257.t1 MTR_3g107160 27.206 272 164 10 9 267 133 383 3.91e-18 83.6
MS.gene43257.t1 MTR_3g026920 26.984 252 128 7 9 237 163 381 4.65e-18 83.2
MS.gene43257.t1 MTR_1g107385 27.206 272 166 9 9 264 165 420 1.23e-17 82.4
MS.gene43257.t1 MTR_7g034110 43.878 98 45 3 134 231 217 304 1.67e-17 81.3
MS.gene43257.t1 MTR_2g008320 29.839 248 143 10 5 237 168 399 2.20e-17 81.6
MS.gene43257.t1 MTR_7g022060 28.516 256 131 8 2 256 112 316 4.65e-17 79.7
MS.gene43257.t1 MTR_7g083740 27.854 219 142 4 33 244 135 344 8.89e-17 79.3
MS.gene43257.t1 MTR_2g007550 32.558 172 77 6 93 241 160 315 1.17e-16 79.0
MS.gene43257.t1 MTR_3g108140 46.250 80 40 1 190 266 68 147 1.41e-16 75.9
MS.gene43257.t1 MTR_5g083890 27.240 279 165 9 9 265 141 403 1.84e-16 79.0
MS.gene43257.t1 MTR_7g007340 29.339 242 147 6 5 237 103 329 3.32e-16 77.8
MS.gene43257.t1 MTR_2g007400 30.279 251 136 11 12 241 147 379 3.68e-16 77.8
MS.gene43257.t1 MTR_3g106960 34.615 156 82 7 114 265 54 193 4.07e-16 75.9
MS.gene43257.t1 MTR_2g005990 28.807 243 145 8 10 237 138 367 5.89e-16 77.4
MS.gene43257.t1 MTR_7g021930 28.405 257 130 9 2 256 112 316 1.25e-15 75.9
MS.gene43257.t1 MTR_7g021970 28.405 257 130 9 2 256 112 316 1.25e-15 75.9
MS.gene43257.t1 MTR_7g056280 35.537 121 68 2 121 237 432 546 1.64e-15 76.3
MS.gene43257.t1 MTR_2g036470 26.481 287 169 9 1 264 126 393 2.43e-15 75.5
MS.gene43257.t1 MTR_2g008310 28.514 249 143 9 5 237 114 343 5.17e-15 74.3
MS.gene43257.t1 MTR_1377s0010 36.752 117 63 4 135 246 26 136 6.60e-15 70.1
MS.gene43257.t1 MTR_5g031970 28.346 254 146 6 10 258 122 344 2.48e-14 72.4
MS.gene43257.t1 MTR_6g044572 27.899 276 158 9 6 267 140 388 4.66e-14 71.6
MS.gene43257.t1 MTR_3g099830 28.326 233 150 4 14 237 122 346 5.30e-14 71.2
MS.gene43257.t1 MTR_6g044560 27.899 276 158 9 6 267 316 564 8.12e-14 71.2
MS.gene43257.t1 MTR_2g007360 28.571 266 152 13 19 261 115 365 1.69e-13 70.1
MS.gene43257.t1 MTR_7g075820 25.856 263 167 9 9 261 141 385 2.02e-13 69.7
MS.gene43257.t1 MTR_4g096730 43.902 82 38 2 190 267 12 89 2.10e-13 65.1
MS.gene43257.t1 MTR_5g045510 28.929 280 135 13 10 264 129 369 2.28e-13 69.7
MS.gene43257.t1 MTR_2g007090 28.511 235 123 12 44 266 92 293 3.50e-13 68.6
MS.gene43257.t1 MTR_7g034090 32.278 158 92 6 115 267 165 312 6.62e-13 68.6
MS.gene43257.t1 MTR_3g108560 31.285 179 111 6 5 174 122 297 9.70e-13 67.4
MS.gene43257.t1 MTR_5g024060 33.088 136 80 5 125 260 74 198 2.14e-12 65.9
MS.gene43257.t1 MTR_5g039470 26.613 248 143 9 6 232 97 326 4.49e-12 65.5
MS.gene43257.t1 MTR_3g094980 33.333 90 60 0 154 243 34 123 3.28e-11 60.8
MS.gene43257.t1 MTR_3g071790 26.154 195 123 7 47 231 19 202 5.30e-11 60.8
MS.gene43257.t1 MTR_5g039490 25.275 273 163 6 1 264 133 373 5.80e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene43257.t1 AT5G56370 28.939 311 172 10 3 266 112 420 6.02e-16 77.4
MS.gene43257.t1 AT5G56370 28.939 311 172 10 3 266 112 420 6.02e-16 77.4
MS.gene43257.t1 AT3G52680 27.157 313 162 15 12 267 142 445 1.55e-14 73.2
MS.gene43257.t1 AT5G44850 29.020 255 160 9 8 255 76 316 3.98e-14 71.6
MS.gene43257.t1 AT4G00160 26.316 285 145 13 9 240 135 407 6.82e-14 71.2
MS.gene43257.t1 AT4G26340 26.531 294 161 9 10 256 120 405 7.25e-14 71.2
MS.gene43257.t1 AT3G26930 27.083 288 169 12 4 267 128 398 1.22e-13 70.5
MS.gene43257.t1 AT3G26930 27.083 288 169 12 4 267 173 443 1.23e-13 70.9
MS.gene43257.t1 AT1G32375 25.490 306 160 12 17 267 130 422 2.24e-12 67.0
MS.gene43257.t1 AT1G32375 25.490 306 160 12 17 267 130 422 2.24e-12 67.0
MS.gene43257.t1 AT3G26920 28.409 264 135 13 19 266 114 339 4.89e-12 65.5
MS.gene43257.t1 AT3G26920 28.409 264 135 13 19 266 114 339 4.89e-12 65.5
MS.gene43257.t1 AT5G38580 35.135 111 68 2 159 266 54 163 2.19e-11 61.2

Find 55 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATTTCCCGTTTATATCTA+TGG 0.199933 8.4:-20045850 None:intergenic
TTCCTTTCTTCTTGCTTTCC+AGG 0.207683 8.4:+20045223 MS.gene43257:CDS
AATCAAATGCTTCCCCAATT+TGG 0.243017 8.4:+20045170 MS.gene43257:CDS
TGTTTACAGGAGATAGAAAA+TGG 0.244415 8.4:+20045639 MS.gene43257:intron
AGGCATTCTGGAACAATCTT+TGG 0.254606 8.4:-20045702 None:intergenic
AGCTGAGATGAAAGGCATTC+TGG 0.263550 8.4:-20045714 None:intergenic
AAGCTTCGCTGTCACCAAAT+TGG 0.267779 8.4:-20045184 None:intergenic
AGCTTCGCTGTCACCAAATT+GGG 0.276716 8.4:-20045183 None:intergenic
AGAAACTATCCCTGTGTCTA+AGG 0.292369 8.4:+20045883 MS.gene43257:CDS
CTTCCAACATATTCACTTGT+AGG 0.295079 8.4:+20044935 MS.gene43257:CDS
TAATGTAAATGTATATCCTT+TGG 0.339583 8.4:+20045131 MS.gene43257:CDS
GAAACGATGATGTGGTCATT+TGG 0.379648 8.4:+20045673 MS.gene43257:CDS
CCCGTTTATATCTATGGAAC+TGG 0.388812 8.4:-20045844 None:intergenic
GGCATTCTGGAACAATCTTT+GGG 0.400800 8.4:-20045701 None:intergenic
TCCTTTCTTCTTGCTTTCCA+GGG 0.406059 8.4:+20045224 MS.gene43257:CDS
ATGGTAAGATGTTGAAGCTT+TGG 0.432228 8.4:-20045483 None:intergenic
TCCAGTTCCATAGATATAAA+CGG 0.433714 8.4:+20045843 MS.gene43257:CDS
GAAAGCAAGAAGAAAGGAAT+AGG 0.436564 8.4:-20045219 None:intergenic
TGCTTGTTTAAGAATTATAG+AGG 0.448930 8.4:+20045744 MS.gene43257:CDS
AATGTAAATGTATATCCTTT+GGG 0.451184 8.4:+20045132 MS.gene43257:CDS
ATAGAAAATGGGACAGAGAA+TGG 0.455331 8.4:+20045651 MS.gene43257:CDS
AAACGATGATGTGGTCATTT+GGG 0.458686 8.4:+20045674 MS.gene43257:CDS
GAAATAGTATTATTTATTTG+AGG 0.461286 8.4:-20045006 None:intergenic
AAATGGGGACTTGGACATAG+TGG 0.464992 8.4:-20045365 None:intergenic
TTTATGTGAAACCTCGTGAA+TGG 0.469715 8.4:-20045502 None:intergenic
CCAGTTCCATAGATATAAAC+GGG 0.470516 8.4:+20045844 MS.gene43257:CDS
TGTTTGCAATTCCTCTAGGA+TGG 0.472265 8.4:-20045106 None:intergenic
AATGGAGCTATTCTCTAACT+TGG 0.485384 8.4:+20045404 MS.gene43257:CDS
GGGATAGTTTCTGTAACATC+TGG 0.498285 8.4:-20045872 None:intergenic
CTAACTTGGAGCATAAAAGT+TGG 0.509469 8.4:+20045418 MS.gene43257:CDS
TATTTGTTTGCAATTCCTCT+AGG 0.511366 8.4:-20045110 None:intergenic
GAAACTATCCCTGTGTCTAA+GGG 0.516591 8.4:+20045884 MS.gene43257:CDS
GTTTGCAATTCCTCTAGGAT+GGG 0.523018 8.4:-20045105 None:intergenic
GCTTACATCCCCTTAGACAC+AGG 0.529221 8.4:-20045893 None:intergenic
ACCCTGGAAAGCAAGAAGAA+AGG 0.536890 8.4:-20045225 None:intergenic
TTATTTGAGGAATGTCTTCG+AGG 0.545174 8.4:-20044993 None:intergenic
TGCTGAAATAAACACTATCC+AGG 0.553370 8.4:-20045047 None:intergenic
CAATTCCTCTAGGATGGGAA+AGG 0.554012 8.4:-20045100 None:intergenic
GTTTACAGGAGATAGAAAAT+GGG 0.562565 8.4:+20045640 MS.gene43257:intron
ACTCAGTCTCAGTATAAAGA+TGG 0.566276 8.4:+20045248 MS.gene43257:CDS
ACCACTCAAAACTCTCCACC+TGG 0.585252 8.4:+20045029 MS.gene43257:CDS
TGTCCTACAAGTGAATATGT+TGG 0.588152 8.4:-20044938 None:intergenic
TTTCGAACAAACTTTCCCAA+AGG 0.590464 8.4:-20045147 None:intergenic
GTTGGCGTAAGAAGTGGATG+TGG 0.592904 8.4:+20045436 MS.gene43257:CDS
AATGGGGACTTGGACATAGT+GGG 0.607464 8.4:-20045364 None:intergenic
CTTATCCTTTCCCATCCTAG+AGG 0.610530 8.4:+20045095 MS.gene43257:CDS
GCTTCGCTGTCACCAAATTG+GGG 0.619945 8.4:-20045182 None:intergenic
ATAAAAGTTGGCGTAAGAAG+TGG 0.630364 8.4:+20045430 MS.gene43257:CDS
AAACTATCCCTGTGTCTAAG+GGG 0.639086 8.4:+20045885 MS.gene43257:CDS
CGATGATGTGGTCATTTGGG+AGG 0.641554 8.4:+20045677 MS.gene43257:CDS
AGAGAATGGAAACGATGATG+TGG 0.642185 8.4:+20045665 MS.gene43257:CDS
CTTACATCCCCTTAGACACA+GGG 0.662755 8.4:-20045892 None:intergenic
TCAACATCTTACCATTCACG+AGG 0.690176 8.4:+20045491 MS.gene43257:CDS
TATACTGAGACTGAGTACCC+TGG 0.691980 8.4:-20045241 None:intergenic
TGAAATAAACACTATCCAGG+TGG 0.720550 8.4:-20045044 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAATAGTATTATTTATTTG+AGG - chr8.4:20045009-20045028 None:intergenic 15.0%
!! AATGTAAATGTATATCCTTT+GGG + chr8.4:20045132-20045151 MS.gene43257:CDS 20.0%
!! AGTTAAACAAAATAGATATC+GGG - chr8.4:20045541-20045560 None:intergenic 20.0%
!! GAAATTTACTTATTGTTTAC+AGG + chr8.4:20045626-20045645 MS.gene43257:intron 20.0%
!! GAGTTAAACAAAATAGATAT+CGG - chr8.4:20045542-20045561 None:intergenic 20.0%
!! TAATGTAAATGTATATCCTT+TGG + chr8.4:20045131-20045150 MS.gene43257:CDS 20.0%
!! TGAGTTAGATTGTAAAAAAT+GGG - chr8.4:20045384-20045403 None:intergenic 20.0%
!! TTGAGTTAGATTGTAAAAAA+TGG - chr8.4:20045385-20045404 None:intergenic 20.0%
!!! ATATCTTAACTTTTGGTTTT+TGG + chr8.4:20045571-20045590 MS.gene43257:intron 20.0%
!!! TTACTCTTAACTTTTGTTTT+TGG + chr8.4:20045601-20045620 MS.gene43257:intron 20.0%
!!! TTTTTACAATCTAACTCAAA+TGG + chr8.4:20045386-20045405 MS.gene43257:CDS 20.0%
! GAGTTAGATTGTAAAAAATG+GGG - chr8.4:20045383-20045402 None:intergenic 25.0%
! TGCTTGTTTAAGAATTATAG+AGG + chr8.4:20045744-20045763 MS.gene43257:CDS 25.0%
!! CATCTACATATCTTAACTTT+TGG + chr8.4:20045564-20045583 MS.gene43257:intron 25.0%
GTATTTCCCGTTTATATCTA+TGG - chr8.4:20045853-20045872 None:intergenic 30.0%
GTCTGCATGAATTACAAAAA+CGG - chr8.4:20045339-20045358 None:intergenic 30.0%
GTTTACAGGAGATAGAAAAT+GGG + chr8.4:20045640-20045659 MS.gene43257:intron 30.0%
TATTTGTTTGCAATTCCTCT+AGG - chr8.4:20045113-20045132 None:intergenic 30.0%
TCCAGTTCCATAGATATAAA+CGG + chr8.4:20045843-20045862 MS.gene43257:CDS 30.0%
TCTGCATGAATTACAAAAAC+GGG - chr8.4:20045338-20045357 None:intergenic 30.0%
TGTTTACAGGAGATAGAAAA+TGG + chr8.4:20045639-20045658 MS.gene43257:intron 30.0%
! TTTTGTAATTCATGCAGACA+TGG + chr8.4:20045340-20045359 MS.gene43257:intron 30.0%
!!! AAGTTTTTAGCTGAGATGAA+AGG - chr8.4:20045725-20045744 None:intergenic 30.0%
!!! CTCTTAACTTTTGTTTTTGG+TGG + chr8.4:20045604-20045623 MS.gene43257:intron 30.0%
!!! TCTTAACTTTTGGTTTTTGG+TGG + chr8.4:20045574-20045593 MS.gene43257:intron 30.0%
AAACGATGATGTGGTCATTT+GGG + chr8.4:20045674-20045693 MS.gene43257:CDS 35.0%
AATCAAATGCTTCCCCAATT+TGG + chr8.4:20045170-20045189 MS.gene43257:CDS 35.0%
AATGGAGCTATTCTCTAACT+TGG + chr8.4:20045404-20045423 MS.gene43257:CDS 35.0%
ACTCAGTCTCAGTATAAAGA+TGG + chr8.4:20045248-20045267 MS.gene43257:CDS 35.0%
ATAAAAGTTGGCGTAAGAAG+TGG + chr8.4:20045430-20045449 MS.gene43257:CDS 35.0%
ATAGAAAATGGGACAGAGAA+TGG + chr8.4:20045651-20045670 MS.gene43257:CDS 35.0%
ATGGTAAGATGTTGAAGCTT+TGG - chr8.4:20045486-20045505 None:intergenic 35.0%
CCAGTTCCATAGATATAAAC+GGG + chr8.4:20045844-20045863 MS.gene43257:CDS 35.0%
CTAACTTGGAGCATAAAAGT+TGG + chr8.4:20045418-20045437 MS.gene43257:CDS 35.0%
CTTCCAACATATTCACTTGT+AGG + chr8.4:20044935-20044954 MS.gene43257:CDS 35.0%
GAAAGCAAGAAGAAAGGAAT+AGG - chr8.4:20045222-20045241 None:intergenic 35.0%
GATTGTAAAAAATGGGGACT+TGG - chr8.4:20045377-20045396 None:intergenic 35.0%
TGAAATAAACACTATCCAGG+TGG - chr8.4:20045047-20045066 None:intergenic 35.0%
TGCTGAAATAAACACTATCC+AGG - chr8.4:20045050-20045069 None:intergenic 35.0%
TGTCCTACAAGTGAATATGT+TGG - chr8.4:20044941-20044960 None:intergenic 35.0%
TTATTTGAGGAATGTCTTCG+AGG - chr8.4:20044996-20045015 None:intergenic 35.0%
TTTATGTGAAACCTCGTGAA+TGG - chr8.4:20045505-20045524 None:intergenic 35.0%
TTTCGAACAAACTTTCCCAA+AGG - chr8.4:20045150-20045169 None:intergenic 35.0%
AAACTATCCCTGTGTCTAAG+GGG + chr8.4:20045885-20045904 MS.gene43257:CDS 40.0%
AGAAACTATCCCTGTGTCTA+AGG + chr8.4:20045883-20045902 MS.gene43257:CDS 40.0%
AGAGAATGGAAACGATGATG+TGG + chr8.4:20045665-20045684 MS.gene43257:CDS 40.0%
CCCGTTTATATCTATGGAAC+TGG - chr8.4:20045847-20045866 None:intergenic 40.0%
GAAACGATGATGTGGTCATT+TGG + chr8.4:20045673-20045692 MS.gene43257:CDS 40.0%
GAAACTATCCCTGTGTCTAA+GGG + chr8.4:20045884-20045903 MS.gene43257:CDS 40.0%
GGGATAGTTTCTGTAACATC+TGG - chr8.4:20045875-20045894 None:intergenic 40.0%
TCAACATCTTACCATTCACG+AGG + chr8.4:20045491-20045510 MS.gene43257:CDS 40.0%
TCCTTTCTTCTTGCTTTCCA+GGG + chr8.4:20045224-20045243 MS.gene43257:CDS 40.0%
TTCCTTTCTTCTTGCTTTCC+AGG + chr8.4:20045223-20045242 MS.gene43257:CDS 40.0%
! TGTTTGCAATTCCTCTAGGA+TGG - chr8.4:20045109-20045128 None:intergenic 40.0%
!! AGGCATTCTGGAACAATCTT+TGG - chr8.4:20045705-20045724 None:intergenic 40.0%
!! GGCATTCTGGAACAATCTTT+GGG - chr8.4:20045704-20045723 None:intergenic 40.0%
!! GTTTGCAATTCCTCTAGGAT+GGG - chr8.4:20045108-20045127 None:intergenic 40.0%
AAATGGGGACTTGGACATAG+TGG - chr8.4:20045368-20045387 None:intergenic 45.0%
AAGCTTCGCTGTCACCAAAT+TGG - chr8.4:20045187-20045206 None:intergenic 45.0%
AATGGGGACTTGGACATAGT+GGG - chr8.4:20045367-20045386 None:intergenic 45.0%
ACCCTGGAAAGCAAGAAGAA+AGG - chr8.4:20045228-20045247 None:intergenic 45.0%
AGCTTCGCTGTCACCAAATT+GGG - chr8.4:20045186-20045205 None:intergenic 45.0%
CTTACATCCCCTTAGACACA+GGG - chr8.4:20045895-20045914 None:intergenic 45.0%
CTTATCCTTTCCCATCCTAG+AGG + chr8.4:20045095-20045114 MS.gene43257:CDS 45.0%
TATACTGAGACTGAGTACCC+TGG - chr8.4:20045244-20045263 None:intergenic 45.0%
!! AGCTGAGATGAAAGGCATTC+TGG - chr8.4:20045717-20045736 None:intergenic 45.0%
!! CAATTCCTCTAGGATGGGAA+AGG - chr8.4:20045103-20045122 None:intergenic 45.0%
ACCACTCAAAACTCTCCACC+TGG + chr8.4:20045029-20045048 MS.gene43257:CDS 50.0%
CGATGATGTGGTCATTTGGG+AGG + chr8.4:20045677-20045696 MS.gene43257:CDS 50.0%
GCTTACATCCCCTTAGACAC+AGG - chr8.4:20045896-20045915 None:intergenic 50.0%
GCTTCGCTGTCACCAAATTG+GGG - chr8.4:20045185-20045204 None:intergenic 50.0%
GTTGGCGTAAGAAGTGGATG+TGG + chr8.4:20045436-20045455 MS.gene43257:CDS 50.0%
!!! TCCAGGTGGAGAGTTTTGAG+TGG - chr8.4:20045033-20045052 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 20044904 20045929 20044904 ID=MS.gene43257
chr8.4 mRNA 20044904 20045929 20044904 ID=MS.gene43257.t1;Parent=MS.gene43257
chr8.4 exon 20044904 20045269 20044904 ID=MS.gene43257.t1.exon1;Parent=MS.gene43257.t1
chr8.4 CDS 20044904 20045269 20044904 ID=cds.MS.gene43257.t1;Parent=MS.gene43257.t1
chr8.4 exon 20045357 20045512 20045357 ID=MS.gene43257.t1.exon2;Parent=MS.gene43257.t1
chr8.4 CDS 20045357 20045512 20045357 ID=cds.MS.gene43257.t1;Parent=MS.gene43257.t1
chr8.4 exon 20045648 20045929 20045648 ID=MS.gene43257.t1.exon3;Parent=MS.gene43257.t1
chr8.4 CDS 20045648 20045929 20045648 ID=cds.MS.gene43257.t1;Parent=MS.gene43257.t1
Gene Sequence

>MS.gene43257

ATGTTTTTTCATACTTTCGACATGAAATTAGCTTCCAACATATTCACTTGTAGGACACTTACTGTTCTCAAATTACAACGCGTAACAATCCTCGAAGACATTCCTCAAATAAATAATACTATTTCACCACTCAAAACTCTCCACCTGGATAGTGTTTATTTCAGCACTCGTACACAGCTCATCGACTTTCTCTTATCCTTTCCCATCCTAGAGGAATTGCAAACAAATAATGTAAATGTATATCCTTTGGGAAAGTTTGTTCGAAAAATCAAATGCTTCCCCAATTTGGTGACAGCGAAGCTTTCTGATAACGAACCTATTCCTTTCTTCTTGCTTTCCAGGGTACTCAGTCTCAGTATAAAGATGGTAAGTAAAAGTTTGTTTGATTTTACAAAACAAATCACAACATGATGATTGATTTCTAATGTTTTCCCGTTTTTGTAATTCATGCAGACATGGACCCACTATGTCCAAGTCCCCATTTTTTACAATCTAACTCAAATGGAGCTATTCTCTAACTTGGAGCATAAAAGTTGGCGTAAGAAGTGGATGTGGATGCTAGAAATGCTTCAACACAGTCCAAAGCTTCAACATCTTACCATTCACGAGGTTTCACATAAAATTTGATTGATTTCCCGATATCTATTTTGTTTAACTCTACATCTACATATCTTAACTTTTGGTTTTTGGTGGAAATTTACTCTTAACTTTTGTTTTTGGTGGAAATTTACTTATTGTTTACAGGAGATAGAAAATGGGACAGAGAATGGAAACGATGATGTGGTCATTTGGGAGGACCCAAAGATTGTTCCAGAATGCCTTTCATCTCAGCTAAAAACTTGCTTGTTTAAGAATTATAGAGGCAAAATGTGTGAGCTTCAATTTGCAGAATATGTTATGCGCAATTCAAAAGTATTAAGTAACATGACAATTCACACTTCCAGTTCCATAGATATAAACGGGAAATACCAGATGTTACAGAAACTATCCCTGTGTCTAAGGGGATGTAAGCTTGTATTTGAATGA
Protein sequence

>MS.gene43257.t1

MFFHTFDMKLASNIFTCRTLTVLKLQRVTILEDIPQINNTISPLKTLHLDSVYFSTRTQLIDFLLSFPILEELQTNNVNVYPLGKFVRKIKCFPNLVTAKLSDNEPIPFFLLSRVLSLSIKMTWTHYVQVPIFYNLTQMELFSNLEHKSWRKKWMWMLEMLQHSPKLQHLTIHEEIENGTENGNDDVVIWEDPKIVPECLSSQLKTCLFKNYRGKMCELQFAEYVMRNSKVLSNMTIHTSSSIDINGKYQMLQKLSLCLRGCKLVFE