AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63224

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63224.t1 MTR_8g062270 70.283 424 112 5 1 421 41 453 0.0 572
MS.gene63224.t1 MTR_8g468840 72.170 424 102 7 2 422 40 450 0.0 560
MS.gene63224.t1 MTR_5g092960 67.056 428 121 6 3 422 47 462 0.0 520
MS.gene63224.t1 MTR_8g468760 66.745 427 94 10 1 421 34 418 0.0 513
MS.gene63224.t1 MTR_8g467800 55.504 427 163 9 2 421 46 452 5.15e-146 423
MS.gene63224.t1 MTR_4g032730 54.773 440 164 7 3 422 43 467 2.74e-142 414
MS.gene63224.t1 MTR_5g092960 67.062 337 92 5 3 331 47 372 2.55e-134 391
MS.gene63224.t1 MTR_8g468810 51.494 435 174 13 1 421 51 462 3.93e-130 383
MS.gene63224.t1 MTR_2g083840 51.991 427 178 8 3 421 49 456 1.05e-125 372
MS.gene63224.t1 MTR_8g058970 46.578 453 189 15 1 421 45 476 5.20e-109 330
MS.gene63224.t1 MTR_8g468810 51.917 339 138 9 1 331 51 372 1.81e-98 299
MS.gene63224.t1 MTR_8g014260 42.958 426 216 11 4 421 44 450 4.09e-96 296
MS.gene63224.t1 MTR_8g445780 44.855 379 173 12 4 376 41 389 2.55e-86 268
MS.gene63224.t1 MTR_8g468810 54.212 273 108 6 1 272 51 307 8.60e-83 257
MS.gene63224.t1 MTR_8g468810 54.212 273 108 6 1 272 51 307 9.18e-83 257
MS.gene63224.t1 MTR_8g468810 54.212 273 108 6 1 272 51 307 1.86e-82 257
MS.gene63224.t1 MTR_8g467800 52.318 302 121 7 2 299 46 328 6.87e-82 255
MS.gene63224.t1 MTR_8g469020 37.986 437 234 11 2 421 34 450 2.03e-73 237
MS.gene63224.t1 MTR_8g058965 38.621 435 233 9 2 421 40 455 1.90e-71 232
MS.gene63224.t1 MTR_8g070950 38.605 430 218 13 1 421 41 433 1.14e-67 221
MS.gene63224.t1 MTR_8g062310 37.292 421 214 12 4 421 54 427 3.42e-65 215
MS.gene63224.t1 MTR_8g062080 37.182 433 223 14 1 421 49 444 6.87e-65 214
MS.gene63224.t1 MTR_4g050250 39.119 386 203 10 1 368 47 418 4.46e-63 209
MS.gene63224.t1 MTR_4g024710 41.456 316 171 9 110 421 41 346 3.45e-61 202
MS.gene63224.t1 MTR_8g062010 37.471 435 218 14 1 421 35 429 9.34e-60 201
MS.gene63224.t1 MTR_8g014210 49.145 234 110 5 190 421 4 230 3.29e-58 190
MS.gene63224.t1 MTR_8g014160 35.831 427 198 13 2 421 67 424 1.43e-55 189
MS.gene63224.t1 MTR_8g062300 34.725 455 233 16 1 421 52 476 4.24e-55 190
MS.gene63224.t1 MTR_8g077460 33.176 425 189 14 4 421 38 374 4.99e-53 181
MS.gene63224.t1 MTR_4g024745 44.444 279 143 8 120 394 2 272 5.61e-53 179
MS.gene63224.t1 MTR_8g467790 33.770 382 173 15 1 366 174 491 2.26e-43 159
MS.gene63224.t1 MTR_5g084070 29.454 421 249 16 2 414 39 419 7.16e-27 112
MS.gene63224.t1 MTR_2g086020 43.056 144 76 2 280 421 175 314 3.31e-24 102
MS.gene63224.t1 MTR_2g086020 37.107 159 73 6 1 154 1 137 9.26e-14 72.4
MS.gene63224.t1 MTR_5g080710 30.660 424 247 18 2 414 59 446 1.49e-22 99.8
MS.gene63224.t1 MTR_8g014290 45.669 127 55 6 4 121 38 159 2.31e-22 93.6
MS.gene63224.t1 MTR_5g021550 45.082 122 57 4 4 121 47 162 3.64e-21 90.1
MS.gene63224.t1 MTR_2g007450 26.077 441 238 17 2 419 42 417 6.28e-20 91.7
MS.gene63224.t1 MTR_4g056070 30.749 374 209 17 25 375 89 435 2.98e-19 89.7
MS.gene63224.t1 MTR_4g056040 29.923 391 226 16 22 391 78 441 1.51e-18 88.2
MS.gene63224.t1 MTR_4g113810 36.723 177 97 5 63 234 2 168 3.69e-17 79.0
MS.gene63224.t1 MTR_7g032370 23.529 425 231 14 2 421 16 351 5.18e-16 79.3
MS.gene63224.t1 MTR_4g098590 24.697 413 205 16 2 391 25 354 1.32e-15 78.2
MS.gene63224.t1 MTR_4g056020 29.282 362 221 13 46 391 69 411 1.34e-15 79.0
MS.gene63224.t1 MTR_4g056030 28.729 362 223 14 46 391 69 411 7.76e-15 76.6
MS.gene63224.t1 MTR_4g055990 27.865 384 235 15 28 391 92 453 8.12e-15 76.6
MS.gene63224.t1 MTR_4g055990 27.865 384 235 15 28 391 92 453 8.68e-15 76.6
MS.gene63224.t1 MTR_1g085120 24.942 429 237 15 2 421 118 470 9.72e-15 76.3
MS.gene63224.t1 MTR_1g085120 24.942 429 237 15 2 421 193 545 1.28e-14 76.3
MS.gene63224.t1 MTR_8g014220 39.394 132 68 4 44 174 40 160 3.15e-14 70.5
MS.gene63224.t1 MTR_6g022500 24.476 429 225 12 5 419 130 473 7.22e-14 73.6
MS.gene63224.t1 MTR_6g011460 24.368 435 228 16 2 421 72 420 7.43e-14 73.2
MS.gene63224.t1 MTR_8g014230 53.448 58 26 1 336 393 19 75 1.61e-13 67.0
MS.gene63224.t1 MTR_4g055580 28.796 382 234 14 25 391 180 538 1.89e-13 72.4
MS.gene63224.t1 MTR_4g024720 38.194 144 65 6 4 128 45 183 4.42e-13 68.6
MS.gene63224.t1 MTR_6g022360 25.648 386 214 13 43 421 88 407 7.47e-12 67.0
MS.gene63224.t1 MTR_5g008950 29.075 227 141 7 213 421 140 364 9.41e-12 66.6
MS.gene63224.t1 MTR_3g087260 27.073 410 230 14 60 421 68 456 1.20e-11 66.6
MS.gene63224.t1 MTR_5g058100 26.909 275 155 12 175 421 127 383 2.17e-11 65.5
MS.gene63224.t1 MTR_3g108560 26.573 286 147 9 144 421 158 388 3.80e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63224.t1 AT4G26340 27.880 434 256 19 2 421 28 418 2.42e-26 110
MS.gene63224.t1 AT1G69630 28.539 445 243 20 2 415 44 444 1.07e-25 108
MS.gene63224.t1 AT5G56420 26.959 434 259 17 2 421 32 421 1.96e-25 107
MS.gene63224.t1 AT5G56420 26.959 434 259 17 2 421 32 421 1.96e-25 107
MS.gene63224.t1 AT5G56420 26.959 434 259 17 2 421 32 421 1.96e-25 107
MS.gene63224.t1 AT5G53635 24.829 439 270 16 3 421 27 425 9.04e-24 103
MS.gene63224.t1 AT5G53840 24.654 434 277 15 2 422 44 440 1.50e-23 102
MS.gene63224.t1 AT5G56440 28.153 444 253 15 2 421 28 429 4.38e-23 101
MS.gene63224.t1 AT1G05080 24.885 434 278 14 3 422 40 439 9.07e-23 100
MS.gene63224.t1 AT4G10420 28.530 347 213 12 83 421 2 321 1.16e-22 98.2
MS.gene63224.t1 AT1G78750 27.578 446 261 20 2 419 44 455 3.25e-22 99.0
MS.gene63224.t1 AT1G78750 27.578 446 261 20 2 419 44 455 3.25e-22 99.0
MS.gene63224.t1 AT1G06630 27.928 333 211 11 2 325 38 350 1.43e-20 92.8
MS.gene63224.t1 AT1G06630 27.928 333 211 11 2 325 38 350 1.43e-20 92.8
MS.gene63224.t1 AT1G06630 27.628 333 212 10 2 325 38 350 2.50e-20 92.8
MS.gene63224.t1 AT1G06630 27.628 333 212 10 2 325 38 350 2.50e-20 92.8
MS.gene63224.t1 AT1G06630 27.628 333 212 10 2 325 38 350 2.50e-20 92.8
MS.gene63224.t1 AT1G06630 27.628 333 212 10 2 325 38 350 2.50e-20 92.8
MS.gene63224.t1 AT5G38590 28.780 410 224 20 2 393 28 387 2.58e-20 92.8
MS.gene63224.t1 AT1G06630 27.628 333 212 10 2 325 47 359 2.77e-20 92.8
MS.gene63224.t1 AT5G56820 31.118 331 176 14 117 419 117 423 2.78e-20 92.8
MS.gene63224.t1 AT1G06630 27.628 333 212 10 2 325 38 350 5.63e-20 92.4
MS.gene63224.t1 AT2G26030 25.055 451 263 20 2 419 30 438 5.73e-20 92.0
MS.gene63224.t1 AT2G26030 25.055 451 263 20 2 419 30 438 5.73e-20 92.0
MS.gene63224.t1 AT5G56700 26.431 367 230 13 46 403 22 357 5.86e-20 91.3
MS.gene63224.t1 AT5G56400 27.689 437 257 18 2 421 60 454 1.51e-19 90.9
MS.gene63224.t1 AT5G60610 27.160 324 210 7 73 388 78 383 1.79e-19 90.1
MS.gene63224.t1 AT5G60610 27.160 324 210 7 73 388 78 383 1.79e-19 90.1
MS.gene63224.t1 AT2G26030 24.667 450 271 19 2 419 30 443 1.97e-19 90.5
MS.gene63224.t1 AT5G56560 26.959 434 253 18 3 421 33 417 2.36e-19 90.1
MS.gene63224.t1 AT1G06630 28.986 276 177 9 2 276 38 295 3.85e-19 87.8
MS.gene63224.t1 AT3G26930 28.507 442 252 21 1 422 1 398 5.03e-19 89.0
MS.gene63224.t1 AT4G10400 24.476 429 267 14 2 421 28 408 5.79e-19 88.6
MS.gene63224.t1 AT4G10400 24.476 429 267 14 2 421 28 408 5.79e-19 88.6
MS.gene63224.t1 AT4G10400 24.476 429 267 14 2 421 28 408 5.79e-19 88.6
MS.gene63224.t1 AT5G56380 26.174 447 267 19 3 422 29 439 6.38e-19 89.0
MS.gene63224.t1 AT5G56380 26.174 447 267 19 3 422 29 439 6.38e-19 89.0
MS.gene63224.t1 AT3G26930 28.700 446 246 23 1 422 46 443 6.65e-19 89.0
MS.gene63224.t1 AT1G16930 24.889 450 262 19 2 419 41 446 7.58e-19 88.6
MS.gene63224.t1 AT1G16930 24.889 450 262 19 2 419 41 446 7.58e-19 88.6
MS.gene63224.t1 AT1G16930 24.889 450 262 19 2 419 41 446 7.58e-19 88.6
MS.gene63224.t1 AT1G78760 23.423 444 269 17 2 419 42 440 1.02e-18 88.2
MS.gene63224.t1 AT3G50710 26.267 434 265 16 3 419 29 424 1.23e-18 87.8
MS.gene63224.t1 AT1G32375 25.115 434 269 16 3 421 29 421 6.98e-18 85.5
MS.gene63224.t1 AT1G32375 25.115 434 269 16 3 421 29 421 6.98e-18 85.5
MS.gene63224.t1 AT1G66300 24.161 447 260 19 3 419 56 453 2.84e-17 84.0
MS.gene63224.t1 AT5G38570 26.823 384 206 11 46 421 59 375 3.59e-17 83.2
MS.gene63224.t1 AT1G51370 23.666 431 269 13 2 417 45 430 4.48e-17 83.2
MS.gene63224.t1 AT1G22000 25.664 452 245 20 2 419 55 449 4.95e-17 83.2
MS.gene63224.t1 AT2G26030 24.873 394 234 17 59 419 1 365 6.07e-17 82.4
MS.gene63224.t1 AT5G22670 25.000 404 251 15 2 387 37 406 1.89e-16 81.3
MS.gene63224.t1 AT4G09920 27.719 285 162 13 2 276 28 278 2.15e-16 80.1
MS.gene63224.t1 AT4G09920 27.719 285 162 13 2 276 28 278 2.15e-16 80.1
MS.gene63224.t1 AT1G66310 25.513 439 262 17 2 419 45 439 4.99e-16 80.1
MS.gene63224.t1 AT4G09920 27.526 287 164 13 2 278 28 280 5.86e-16 78.6
MS.gene63224.t1 AT4G09920 27.526 287 164 13 2 278 28 280 5.86e-16 78.6
MS.gene63224.t1 AT4G09920 27.526 287 164 13 2 278 28 280 5.86e-16 78.6
MS.gene63224.t1 AT1G55660 25.682 440 282 17 2 422 79 492 1.36e-15 79.0
MS.gene63224.t1 AT3G49480 27.181 298 176 11 108 391 1 271 2.28e-15 77.0
MS.gene63224.t1 AT4G00315 25.225 444 279 17 2 422 28 441 3.55e-15 77.4
MS.gene63224.t1 AT2G26860 26.034 411 235 18 2 388 28 393 4.09e-15 77.0
MS.gene63224.t1 AT2G26860 26.034 411 235 18 2 388 28 393 4.09e-15 77.0
MS.gene63224.t1 AT3G49480 27.090 299 177 11 107 391 140 411 5.11e-15 77.0
MS.gene63224.t1 AT3G49030 25.866 433 245 15 2 422 47 415 8.96e-15 76.3
MS.gene63224.t1 AT5G56810 27.438 441 247 17 2 419 41 431 2.20e-14 75.1
MS.gene63224.t1 AT3G49020 26.077 441 263 18 2 422 48 445 2.31e-14 75.1
MS.gene63224.t1 AT5G18770 23.982 442 282 17 2 420 50 460 3.43e-14 74.7
MS.gene63224.t1 AT5G22610 24.161 447 285 18 2 420 44 464 4.47e-14 74.3
MS.gene63224.t1 AT5G22610 24.018 433 273 18 2 420 44 434 9.75e-14 73.2
MS.gene63224.t1 AT5G22720 24.946 461 260 22 2 421 49 464 1.07e-13 73.2
MS.gene63224.t1 AT3G54910 27.273 385 239 15 45 421 21 372 1.13e-13 72.4
MS.gene63224.t1 AT3G54910 27.273 385 239 15 45 421 21 372 1.13e-13 72.4
MS.gene63224.t1 AT3G54910 27.273 385 239 15 45 421 21 372 1.13e-13 72.4
MS.gene63224.t1 AT5G56325 29.740 269 160 14 166 421 11 263 1.42e-13 70.9
MS.gene63224.t1 AT5G22700 24.331 411 247 14 2 387 32 403 2.01e-13 72.0
MS.gene63224.t1 AT5G22700 24.331 411 247 14 2 387 32 403 2.01e-13 72.0
MS.gene63224.t1 AT5G22700 24.331 411 247 14 2 387 32 403 2.01e-13 72.0
MS.gene63224.t1 AT5G22700 24.331 411 247 14 2 387 32 403 2.01e-13 72.0
MS.gene63224.t1 AT5G56390 24.419 430 279 18 3 421 30 424 2.62e-13 71.6
MS.gene63224.t1 AT5G56560 30.822 292 163 14 146 421 338 606 3.23e-13 72.0
MS.gene63224.t1 AT2G04230 26.980 404 239 17 2 387 39 404 3.29e-13 71.6
MS.gene63224.t1 AT3G26922 28.470 281 159 11 2 274 11 257 3.45e-13 70.1
MS.gene63224.t1 AT3G26922 28.470 281 159 11 2 274 11 257 3.45e-13 70.1
MS.gene63224.t1 AT5G22700 24.331 411 247 14 2 387 69 440 3.46e-13 71.6
MS.gene63224.t1 AT5G56800 28.873 284 176 10 148 422 78 344 3.97e-13 70.5
MS.gene63224.t1 AT1G66290 24.722 449 254 22 2 419 55 450 4.73e-13 70.9
MS.gene63224.t1 AT3G26922 28.470 281 159 11 2 274 40 286 5.48e-13 69.7
MS.gene63224.t1 AT3G26922 28.470 281 159 11 2 274 40 286 5.48e-13 69.7
MS.gene63224.t1 AT5G44980 24.208 442 275 19 2 421 30 433 8.62e-13 70.1
MS.gene63224.t1 AT5G44980 24.208 442 275 19 2 421 30 433 8.62e-13 70.1
MS.gene63224.t1 AT5G56690 23.529 408 258 17 2 391 28 399 1.06e-12 69.7
MS.gene63224.t1 AT4G13965 28.358 402 222 20 2 387 36 387 1.25e-12 69.7
MS.gene63224.t1 AT1G66320 24.558 452 254 19 2 419 56 454 1.31e-12 69.7
MS.gene63224.t1 AT5G62970 23.214 448 290 16 2 422 29 449 1.65e-12 69.3
MS.gene63224.t1 AT3G44810 29.450 309 189 12 2 299 33 323 2.38e-12 68.9
MS.gene63224.t1 AT1G55030 27.612 402 230 17 2 390 35 388 4.92e-12 67.8
MS.gene63224.t1 AT1G55030 27.431 401 232 17 2 390 45 398 5.17e-12 67.8
MS.gene63224.t1 AT3G58860 26.010 396 245 18 2 380 33 397 8.64e-12 67.0
MS.gene63224.t1 AT5G22660 26.036 338 194 14 79 387 100 410 1.10e-11 66.6
MS.gene63224.t1 AT4G15060 28.866 291 157 14 2 274 52 310 2.63e-11 65.5
MS.gene63224.t1 AT4G15060 28.866 291 157 14 2 274 198 456 3.31e-11 65.5
MS.gene63224.t1 AT1G80470 21.818 440 276 19 2 419 41 434 3.56e-11 65.1
MS.gene63224.t1 AT5G56570 27.915 283 176 12 146 421 177 438 6.77e-11 64.3
MS.gene63224.t1 AT5G56700 28.302 212 133 8 46 252 22 219 9.29e-11 62.8

Find 107 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTCAACAGATTCACTTTC+AGG 0.185864 8.4:+41899996 None:intergenic
TTAAATGAGCTGGAATTTAT+TGG 0.217075 8.4:-41899461 MS.gene63224:CDS
TTCCTTTCGTCCTGAATCTT+TGG 0.268264 8.4:-41900207 MS.gene63224:CDS
CGTATTATGGCCAAAGATTC+AGG 0.272241 8.4:+41900197 None:intergenic
AGAAGATGGCACCCGTATTA+TGG 0.308259 8.4:+41900184 None:intergenic
CTGTCCAATTCTTGAAAATC+TGG 0.314865 8.4:-41900234 MS.gene63224:CDS
GGAAGCAGATAACGAAATTC+TGG 0.317280 8.4:+41899780 None:intergenic
CTCGCAAATGGCGCAACCTT+TGG 0.324758 8.4:-41901308 MS.gene63224:CDS
ATTAAATTTGAAGCATATCT+AGG 0.335529 8.4:-41899488 MS.gene63224:CDS
CAAATTTAATAAAGGTCAAA+TGG 0.346091 8.4:+41899501 None:intergenic
CTCTTCTCTTGCTCTAACCT+TGG 0.353811 8.4:-41900988 MS.gene63224:CDS
TGTTCGTAGAATACGTCTTC+GGG 0.358206 8.4:+41901228 None:intergenic
CAGCTTGAGATGTTTAATTC+CGG 0.371613 8.4:+41899947 None:intergenic
TTTCGCGATGCCGCCTCTGT+TGG 0.371999 8.4:-41900065 MS.gene63224:CDS
TTCGCGATGCCGCCTCTGTT+GGG 0.380740 8.4:-41900064 MS.gene63224:CDS
CGGCGAAAGGATCGGGATGA+TGG 0.393438 8.4:+41901115 None:intergenic
ATTCTGGAAAATCTAAAGTT+GGG 0.398611 8.4:+41899796 None:intergenic
GAGTGATCGGCGAAAGGATC+GGG 0.402904 8.4:+41901108 None:intergenic
ATATGCTTCAAATTTAATAA+AGG 0.407580 8.4:+41899493 None:intergenic
TTGAGAATATGGTTGGGGTT+TGG 0.409162 8.4:-41900139 MS.gene63224:CDS
TTCGTCGCTCACGCGTCATT+CGG 0.410572 8.4:-41901164 MS.gene63224:CDS
TTTAACCTCTTCAAAGAAGA+TGG 0.415263 8.4:+41900170 None:intergenic
CTGAATCTTTGGCCATAATA+CGG 0.419431 8.4:-41900196 MS.gene63224:CDS
AATTCTGGAAAATCTAAAGT+TGG 0.420845 8.4:+41899795 None:intergenic
TTGTTCGTAGAATACGTCTT+CGG 0.424249 8.4:+41901227 None:intergenic
ACGTGGTATGTTCGAGGCTA+AGG 0.432139 8.4:+41900087 None:intergenic
AAGAGAAGAGTGTGAGAGGC+AGG 0.437300 8.4:+41901001 None:intergenic
CATCTCAATGAAATTTAAGT+TGG 0.444976 8.4:+41899318 None:intergenic
GTTATCAGAGTTTGCAGGAT+AGG 0.455635 8.4:+41899696 None:intergenic
TATGATTCATCACCTGCATA+TGG 0.458368 8.4:-41899566 MS.gene63224:CDS
CACTGTTGAGAATATGGTTG+GGG 0.461928 8.4:-41900144 MS.gene63224:CDS
ATCACTGTTGAGAATATGGT+TGG 0.469967 8.4:-41900146 MS.gene63224:CDS
TCTAGGACATTTAAATGAGC+TGG 0.471859 8.4:-41899471 MS.gene63224:CDS
TTCCTCAAGGTGGGGTCCAA+TGG 0.480195 8.4:+41901062 None:intergenic
AAATTTAATAAAGGTCAAAT+GGG 0.481318 8.4:+41899502 None:intergenic
AAGAGAGAAGAAAGAGCTCT+AGG 0.482206 8.4:+41899757 None:intergenic
TGAGCCTCGTCTCTCGCAAA+TGG 0.483372 8.4:-41901320 MS.gene63224:CDS
TCACTGTTGAGAATATGGTT+GGG 0.483649 8.4:-41900145 MS.gene63224:CDS
GCTCTAACCTTGGTTCCCTC+AGG 0.483970 8.4:-41900978 MS.gene63224:CDS
GGTTTGCTTACCGTCATTGA+AGG 0.489160 8.4:-41900318 MS.gene63224:CDS
AGAGAGAAGAAAGAGCTCTA+GGG 0.504229 8.4:+41899758 None:intergenic
GTTTAATTCCGGAGAGAGCT+CGG 0.505622 8.4:+41899958 None:intergenic
AATCTCCTCCGAGCTCTCTC+CGG 0.506677 8.4:-41899966 MS.gene63224:CDS
CGCGAAACGTGGTATGTTCG+AGG 0.510352 8.4:+41900081 None:intergenic
TGAATGTTATCAGAGTTTGC+AGG 0.511880 8.4:+41899691 None:intergenic
TGAATCTTTGGCCATAATAC+GGG 0.512720 8.4:-41900195 MS.gene63224:CDS
ATTCTATATGATGAACGAAC+CGG 0.512785 8.4:-41899390 MS.gene63224:CDS
TTCATCTTCCATCCATATGC+AGG 0.518979 8.4:+41899554 None:intergenic
GTAATGTACCTCAGTCTCAA+TGG 0.521445 8.4:-41900374 MS.gene63224:intron
GAGGACAGTGTTGACGAAAA+CGG 0.523224 8.4:+41901191 None:intergenic
ACAGATTCACTTTCAGGAGT+GGG 0.541743 8.4:+41900002 None:intergenic
CCACGTGGCAATTGAGTGAT+CGG 0.542746 8.4:+41901095 None:intergenic
AGACACTTAGGAACACTTTG+AGG 0.548550 8.4:+41899530 None:intergenic
GAACGAACCGGAGGAGTGGA+AGG 0.548955 8.4:-41899378 MS.gene63224:CDS
GGCTTGGTAGCCGTCCTCGT+TGG 0.554727 8.4:+41901251 None:intergenic
TGAGTGATCGGCGAAAGGAT+CGG 0.556782 8.4:+41901107 None:intergenic
AAGATGAAATTCCTCAAGGT+GGG 0.557857 8.4:+41901053 None:intergenic
AACAGATTCACTTTCAGGAG+TGG 0.562125 8.4:+41900001 None:intergenic
ATTCATCACCTGCATATGGA+TGG 0.565201 8.4:-41899562 MS.gene63224:CDS
GGCCAAAGATTCAGGACGAA+AGG 0.565919 8.4:+41900205 None:intergenic
AAATGGGATATGAGACACTT+AGG 0.567711 8.4:+41899518 None:intergenic
AATTGGACAGCTCGAGAGAA+GGG 0.568221 8.4:+41900247 None:intergenic
GCAATTGAGTGATCGGCGAA+AGG 0.569421 8.4:+41901102 None:intergenic
GTTGCAATTGCAAGACACTT+CGG 0.571488 8.4:-41900342 MS.gene63224:CDS
GCTGTTTAGTTCAACAACAA+AGG 0.575271 8.4:-41899928 MS.gene63224:intron
GGGTGCCATCTTCTTTGAAG+AGG 0.580167 8.4:-41900175 MS.gene63224:CDS
AGACATCGAGATTCTTCCAA+AGG 0.582544 8.4:+41901292 None:intergenic
ATATTTAAGCCTAACGTGAA+AGG 0.583466 8.4:+41900943 None:intergenic
GAATTGGACAGCTCGAGAGA+AGG 0.583636 8.4:+41900246 None:intergenic
GAGAGAAGAAAGAGCTCTAG+GGG 0.585350 8.4:+41899759 None:intergenic
TGGCACATCGTCGATCCCCT+TGG 0.589391 8.4:+41899338 None:intergenic
GCTCGGAGGAGATTGAGTAA+AGG 0.595653 8.4:+41899975 None:intergenic
GAGCAAGAGAAGAGTGTGAG+AGG 0.596418 8.4:+41900997 None:intergenic
CACGACAACACCAACGAGGA+CGG 0.597782 8.4:-41901261 MS.gene63224:CDS
TGATGAACGAACCGGAGGAG+TGG 0.598627 8.4:-41899382 MS.gene63224:CDS
AGGCAGGTTGTTGAAGCCAG+CGG 0.599981 8.4:+41901017 None:intergenic
TTCTGGAAAATCTAAAGTTG+GGG 0.600216 8.4:+41899797 None:intergenic
ACGTGGATCAGTACTGCCAT+TGG 0.603815 8.4:-41901078 MS.gene63224:CDS
ACTACTAGATATGTATCACT+TGG 0.605468 8.4:-41900288 MS.gene63224:CDS
TTGCTTGCCTGAGGGAACCA+AGG 0.606564 8.4:+41900971 None:intergenic
AGGCTAAGGTACTTGAGACT+AGG 0.612631 8.4:+41900101 None:intergenic
TTCGTAGAATACGTCTTCGG+GGG 0.622157 8.4:+41901230 None:intergenic
GTTCGTAGAATACGTCTTCG+GGG 0.624336 8.4:+41901229 None:intergenic
AAGGAATTGTTGCTTGCCTG+AGG 0.624538 8.4:+41900962 None:intergenic
TAAGATGAAATTCCTCAAGG+TGG 0.630877 8.4:+41901052 None:intergenic
GAGTAAGATGAAATTCCTCA+AGG 0.631074 8.4:+41901049 None:intergenic
CGTCCTCGTTGGTGTTGTCG+TGG 0.631936 8.4:+41901262 None:intergenic
TGCGCCATTTGCGAGAGACG+AGG 0.637473 8.4:+41901316 None:intergenic
TGCCATTGGACCCCACCTTG+AGG 0.641979 8.4:-41901064 MS.gene63224:CDS
AGTTGTAGCACCTTCAATGA+CGG 0.643481 8.4:+41900308 None:intergenic
AGATGAAATTCCTCAAGGTG+GGG 0.650410 8.4:+41901054 None:intergenic
GCAATTGCAAGACACTTCGG+AGG 0.657856 8.4:-41900339 MS.gene63224:CDS
AGGAATTGTTGCTTGCCTGA+GGG 0.659369 8.4:+41900963 None:intergenic
GGATGTGATCGCATGAGAGA+CGG 0.661031 8.4:+41901136 None:intergenic
AGAGGCGGCATCGCGAAACG+TGG 0.661887 8.4:+41900070 None:intergenic
GTACAAGTTCCCAACAGAGG+CGG 0.673883 8.4:+41900055 None:intergenic
GCAGGTGATGAATCATACGA+TGG 0.676320 8.4:+41899572 None:intergenic
CTATATGATGAACGAACCGG+AGG 0.688088 8.4:-41899387 MS.gene63224:CDS
GTTGTACAAGTTCCCAACAG+AGG 0.689541 8.4:+41900052 None:intergenic
TCTGATAACATTCAACGACA+AGG 0.689944 8.4:-41899682 MS.gene63224:intron
AAAATTTCTGATCTGCCAAG+GGG 0.695820 8.4:-41899353 MS.gene63224:CDS
CCGATCACTCAATTGCCACG+TGG 0.708664 8.4:-41901095 MS.gene63224:CDS
CGCGTGAGCGACGAAGAACG+AGG 0.733255 8.4:+41901172 None:intergenic
TAATTCCGGAGAGAGCTCGG+AGG 0.748060 8.4:+41899961 None:intergenic
TGTTGGGAACTTGTACAACG+TGG 0.750385 8.4:-41900048 MS.gene63224:CDS
TTTCCACGACAACACCAACG+AGG 0.769975 8.4:-41901265 MS.gene63224:CDS
AATGGCAGTACTGATCCACG+TGG 0.817792 8.4:+41901080 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTCTTTAAAAATATTTTTT+TGG + chr8.4:41900069-41900088 None:intergenic 10.0%
!! AAATTTAATAAAGGTCAAAT+GGG + chr8.4:41901144-41901163 None:intergenic 15.0%
!! ATATGCTTCAAATTTAATAA+AGG + chr8.4:41901153-41901172 None:intergenic 15.0%
!!! CAAATATTTTACAAATTACA+AGG + chr8.4:41899936-41899955 None:intergenic 15.0%
!!! TATAATTGTTTTTTTTCTTC+AGG - chr8.4:41899767-41899786 MS.gene63224:CDS 15.0%
!! AAATCTACTCATATTGAAAT+TGG + chr8.4:41900002-41900021 None:intergenic 20.0%
!! CAAAATGTTTGATATTTGTA+TGG + chr8.4:41899736-41899755 None:intergenic 20.0%
!! CAAATTTAATAAAGGTCAAA+TGG + chr8.4:41901145-41901164 None:intergenic 20.0%
!! TGAACTAAGTAAAATAATTG+TGG + chr8.4:41900225-41900244 None:intergenic 20.0%
!!! AAAGAAAGTACTAAACAATT+AGG + chr8.4:41900140-41900159 None:intergenic 20.0%
!!! ATTAAATTTGAAGCATATCT+AGG - chr8.4:41901155-41901174 MS.gene63224:CDS 20.0%
!!! CATACAAATATCAAACATTT+TGG - chr8.4:41899734-41899753 MS.gene63224:CDS 20.0%
! AAAAATCACTGTTGAGAATA+TGG - chr8.4:41900493-41900512 MS.gene63224:intron 25.0%
! TTAAAGAGACTAAAACCAAA+AGG - chr8.4:41900080-41900099 MS.gene63224:CDS 25.0%
! TTAAATGAGCTGGAATTTAT+TGG - chr8.4:41901182-41901201 MS.gene63224:CDS 25.0%
!! AATTCTGGAAAATCTAAAGT+TGG + chr8.4:41900851-41900870 None:intergenic 25.0%
!! ATGTTTAGTATCTGTTGTTT+AGG + chr8.4:41900196-41900215 None:intergenic 25.0%
!! ATTCTGGAAAATCTAAAGTT+GGG + chr8.4:41900850-41900869 None:intergenic 25.0%
!! CAATATGAATCAATTTTGCA+AGG + chr8.4:41899831-41899850 None:intergenic 25.0%
!! CAATATGAGTAGATTTTACA+AGG - chr8.4:41900006-41900025 MS.gene63224:CDS 25.0%
!! CTTGCAAAATTGATTCATAT+TGG - chr8.4:41899829-41899848 MS.gene63224:intron 25.0%
!! TGTTTAGTATCTGTTGTTTA+GGG + chr8.4:41900195-41900214 None:intergenic 25.0%
!!! CATTTTAGTCCTTATTTTGA+AGG + chr8.4:41899967-41899986 None:intergenic 25.0%
AAAAAATTTCTGATCTGCCA+AGG - chr8.4:41901288-41901307 MS.gene63224:CDS 30.0%
AAAAATTTCTGATCTGCCAA+GGG - chr8.4:41901289-41901308 MS.gene63224:CDS 30.0%
ACTACTAGATATGTATCACT+TGG - chr8.4:41900355-41900374 MS.gene63224:CDS 30.0%
ATATTTAAGCCTAACGTGAA+AGG + chr8.4:41899703-41899722 None:intergenic 30.0%
ATTCTATATGATGAACGAAC+CGG - chr8.4:41901253-41901272 MS.gene63224:CDS 30.0%
CAAAAGACTCTATATTTGCA+AGG - chr8.4:41900036-41900055 MS.gene63224:CDS 30.0%
CAAAAGGCTTGATATTTACA+AGG - chr8.4:41900096-41900115 MS.gene63224:CDS 30.0%
GATTGAAGTCCTTCAAAATA+AGG - chr8.4:41899955-41899974 MS.gene63224:CDS 30.0%
GGTTTGATTCATATTGGATT+TGG - chr8.4:41899909-41899928 MS.gene63224:intron 30.0%
TTCATATTGGATTTGATCCT+TGG - chr8.4:41899842-41899861 MS.gene63224:intron 30.0%
TTTAACCTCTTCAAAGAAGA+TGG + chr8.4:41900476-41900495 None:intergenic 30.0%
! ATTGGCTATGTTTTGCAAAA+TGG - chr8.4:41901200-41901219 MS.gene63224:CDS 30.0%
! CTTGTAAATATCAAGCCTTT+TGG + chr8.4:41900098-41900117 None:intergenic 30.0%
! TTCTGGAAAATCTAAAGTTG+GGG + chr8.4:41900849-41900868 None:intergenic 30.0%
! TTGCTTGGTTTGATTCATAT+TGG - chr8.4:41899903-41899922 MS.gene63224:intron 30.0%
!! AGTTCCAGATTTTCAAGAAT+TGG + chr8.4:41900416-41900435 None:intergenic 30.0%
AAAATTTCTGATCTGCCAAG+GGG - chr8.4:41901290-41901309 MS.gene63224:CDS 35.0%
AAATGGGATATGAGACACTT+AGG + chr8.4:41901128-41901147 None:intergenic 35.0%
AAGATGAAATTCCTCAAGGT+GGG + chr8.4:41899593-41899612 None:intergenic 35.0%
ATCACTGTTGAGAATATGGT+TGG - chr8.4:41900497-41900516 MS.gene63224:intron 35.0%
ATCACTTGAGCTAAATTGCT+TGG - chr8.4:41899888-41899907 MS.gene63224:intron 35.0%
CAGCTTGAGATGTTTAATTC+CGG + chr8.4:41900699-41900718 None:intergenic 35.0%
CTGAATCTTTGGCCATAATA+CGG - chr8.4:41900447-41900466 MS.gene63224:intron 35.0%
CTGTCCAATTCTTGAAAATC+TGG - chr8.4:41900409-41900428 MS.gene63224:intron 35.0%
GAGTAAGATGAAATTCCTCA+AGG + chr8.4:41899597-41899616 None:intergenic 35.0%
TAAGATGAAATTCCTCAAGG+TGG + chr8.4:41899594-41899613 None:intergenic 35.0%
TATGATTCATCACCTGCATA+TGG - chr8.4:41901077-41901096 MS.gene63224:CDS 35.0%
TCACTGTTGAGAATATGGTT+GGG - chr8.4:41900498-41900517 MS.gene63224:intron 35.0%
TCTGATAACATTCAACGACA+AGG - chr8.4:41900961-41900980 MS.gene63224:CDS 35.0%
TGAATCTTTGGCCATAATAC+GGG - chr8.4:41900448-41900467 MS.gene63224:intron 35.0%
TGAATGTTATCAGAGTTTGC+AGG + chr8.4:41900955-41900974 None:intergenic 35.0%
TTATTAATGAGCTCTGCCAA+AGG + chr8.4:41899869-41899888 None:intergenic 35.0%
TTGTTCGTAGAATACGTCTT+CGG + chr8.4:41899419-41899438 None:intergenic 35.0%
! AATCATACGATGGATTCATC+TGG + chr8.4:41901064-41901083 None:intergenic 35.0%
! GCTGTTTAGTTCAACAACAA+AGG - chr8.4:41900715-41900734 MS.gene63224:intron 35.0%
! TCTAGGACATTTAAATGAGC+TGG - chr8.4:41901172-41901191 MS.gene63224:CDS 35.0%
AACAGATTCACTTTCAGGAG+TGG + chr8.4:41900645-41900664 None:intergenic 40.0%
AAGAGAGAAGAAAGAGCTCT+AGG + chr8.4:41900889-41900908 None:intergenic 40.0%
ACAGATTCACTTTCAGGAGT+GGG + chr8.4:41900644-41900663 None:intergenic 40.0%
AGACACTTAGGAACACTTTG+AGG + chr8.4:41901116-41901135 None:intergenic 40.0%
AGACATCGAGATTCTTCCAA+AGG + chr8.4:41899354-41899373 None:intergenic 40.0%
AGAGAGAAGAAAGAGCTCTA+GGG + chr8.4:41900888-41900907 None:intergenic 40.0%
AGATATAGCCATTGAGACTG+AGG + chr8.4:41900280-41900299 None:intergenic 40.0%
AGATGAAATTCCTCAAGGTG+GGG + chr8.4:41899592-41899611 None:intergenic 40.0%
AGTTGTAGCACCTTCAATGA+CGG + chr8.4:41900338-41900357 None:intergenic 40.0%
ATGGATTCATCTGGCAACAA+GGG + chr8.4:41901055-41901074 None:intergenic 40.0%
ATTCATCACCTGCATATGGA+TGG - chr8.4:41901081-41901100 MS.gene63224:CDS 40.0%
CACTGTTGAGAATATGGTTG+GGG - chr8.4:41900499-41900518 MS.gene63224:intron 40.0%
CGTATTATGGCCAAAGATTC+AGG + chr8.4:41900449-41900468 None:intergenic 40.0%
GCAACAATTCCTTTCACGTT+AGG - chr8.4:41899691-41899710 MS.gene63224:CDS 40.0%
GGAAGCAGATAACGAAATTC+TGG + chr8.4:41900866-41900885 None:intergenic 40.0%
GGTTCAACAGATTCACTTTC+AGG + chr8.4:41900650-41900669 None:intergenic 40.0%
GTAATGTACCTCAGTCTCAA+TGG - chr8.4:41900269-41900288 MS.gene63224:CDS 40.0%
GTTATCAGAGTTTGCAGGAT+AGG + chr8.4:41900950-41900969 None:intergenic 40.0%
GTTGCAATTGCAAGACACTT+CGG - chr8.4:41900301-41900320 MS.gene63224:CDS 40.0%
TGTTCGTAGAATACGTCTTC+GGG + chr8.4:41899418-41899437 None:intergenic 40.0%
TTCATCTTCCATCCATATGC+AGG + chr8.4:41901092-41901111 None:intergenic 40.0%
TTCCTTTCGTCCTGAATCTT+TGG - chr8.4:41900436-41900455 MS.gene63224:intron 40.0%
TTGAGAATATGGTTGGGGTT+TGG - chr8.4:41900504-41900523 MS.gene63224:intron 40.0%
!!! ATTTTTTCCTTCCACTCCTC+CGG + chr8.4:41901275-41901294 None:intergenic 40.0%
AATTGGACAGCTCGAGAGAA+GGG + chr8.4:41900399-41900418 None:intergenic 45.0%
AGAAGATGGCACCCGTATTA+TGG + chr8.4:41900462-41900481 None:intergenic 45.0%
CTATATGATGAACGAACCGG+AGG - chr8.4:41901256-41901275 MS.gene63224:CDS 45.0%
CTCTTCTCTTGCTCTAACCT+TGG - chr8.4:41899655-41899674 MS.gene63224:intron 45.0%
GCAGGTGATGAATCATACGA+TGG + chr8.4:41901074-41901093 None:intergenic 45.0%
GGATTTGATCCTTGGTCCTT+TGG - chr8.4:41899850-41899869 MS.gene63224:intron 45.0%
GTTCGTAGAATACGTCTTCG+GGG + chr8.4:41899417-41899436 None:intergenic 45.0%
GTTGTACAAGTTCCCAACAG+AGG + chr8.4:41900594-41900613 None:intergenic 45.0%
GTTTAATTCCGGAGAGAGCT+CGG + chr8.4:41900688-41900707 None:intergenic 45.0%
TGGATTCATCTGGCAACAAG+GGG + chr8.4:41901054-41901073 None:intergenic 45.0%
TGTTGGGAACTTGTACAACG+TGG - chr8.4:41900595-41900614 MS.gene63224:intron 45.0%
TTCGTAGAATACGTCTTCGG+GGG + chr8.4:41899416-41899435 None:intergenic 45.0%
! AAGGAATTGTTGCTTGCCTG+AGG + chr8.4:41899684-41899703 None:intergenic 45.0%
! AGGAATTGTTGCTTGCCTGA+GGG + chr8.4:41899683-41899702 None:intergenic 45.0%
! AGGCTAAGGTACTTGAGACT+AGG + chr8.4:41900545-41900564 None:intergenic 45.0%
! GAGAGAAGAAAGAGCTCTAG+GGG + chr8.4:41900887-41900906 None:intergenic 45.0%
! GATGGATTCATCTGGCAACA+AGG + chr8.4:41901056-41901075 None:intergenic 45.0%
! GGTTTGCTTACCGTCATTGA+AGG - chr8.4:41900325-41900344 MS.gene63224:CDS 45.0%
!! GAGGACAGTGTTGACGAAAA+CGG + chr8.4:41899455-41899474 None:intergenic 45.0%
ACGTGGATCAGTACTGCCAT+TGG - chr8.4:41899565-41899584 MS.gene63224:CDS 50.0%
ACGTGGTATGTTCGAGGCTA+AGG + chr8.4:41900559-41900578 None:intergenic 50.0%
ATGCTGTCGTGTACTTGCAG+TGG - chr8.4:41900813-41900832 MS.gene63224:intron 50.0%
CCACGTGGCAATTGAGTGAT+CGG + chr8.4:41899551-41899570 None:intergenic 50.0%
GAATTGGACAGCTCGAGAGA+AGG + chr8.4:41900400-41900419 None:intergenic 50.0%
GCAATTGAGTGATCGGCGAA+AGG + chr8.4:41899544-41899563 None:intergenic 50.0%
GCAATTGCAAGACACTTCGG+AGG - chr8.4:41900304-41900323 MS.gene63224:CDS 50.0%
GCTCGGAGGAGATTGAGTAA+AGG + chr8.4:41900671-41900690 None:intergenic 50.0%
GGATGTGATCGCATGAGAGA+CGG + chr8.4:41899510-41899529 None:intergenic 50.0%
GGCCAAAGATTCAGGACGAA+AGG + chr8.4:41900441-41900460 None:intergenic 50.0%
GTACAAGTTCCCAACAGAGG+CGG + chr8.4:41900591-41900610 None:intergenic 50.0%
TGAGTGATCGGCGAAAGGAT+CGG + chr8.4:41899539-41899558 None:intergenic 50.0%
TTTCCACGACAACACCAACG+AGG - chr8.4:41899378-41899397 MS.gene63224:CDS 50.0%
! AAGAGAAGAGTGTGAGAGGC+AGG + chr8.4:41899645-41899664 None:intergenic 50.0%
! CTTACTCTCTTTTCTGCCGC+TGG - chr8.4:41899610-41899629 MS.gene63224:intron 50.0%
! GAGCAAGAGAAGAGTGTGAG+AGG + chr8.4:41899649-41899668 None:intergenic 50.0%
! GGGTGCCATCTTCTTTGAAG+AGG - chr8.4:41900468-41900487 MS.gene63224:intron 50.0%
!! AATGGCAGTACTGATCCACG+TGG + chr8.4:41899566-41899585 None:intergenic 50.0%
AATCTCCTCCGAGCTCTCTC+CGG - chr8.4:41900677-41900696 MS.gene63224:intron 55.0%
AGAATACGTCTTCGGGGGCT+TGG + chr8.4:41899411-41899430 None:intergenic 55.0%
AGGCAGGTTGTTGAAGCCAG+CGG + chr8.4:41899629-41899648 None:intergenic 55.0%
CACGACAACACCAACGAGGA+CGG - chr8.4:41899382-41899401 MS.gene63224:CDS 55.0%
CCGATCACTCAATTGCCACG+TGG - chr8.4:41899548-41899567 MS.gene63224:CDS 55.0%
CGCGAAACGTGGTATGTTCG+AGG + chr8.4:41900565-41900584 None:intergenic 55.0%
CTCGCAAATGGCGCAACCTT+TGG - chr8.4:41899335-41899354 MS.gene63224:CDS 55.0%
GAGTGATCGGCGAAAGGATC+GGG + chr8.4:41899538-41899557 None:intergenic 55.0%
GCTCTAACCTTGGTTCCCTC+AGG - chr8.4:41899665-41899684 MS.gene63224:intron 55.0%
TAATTCCGGAGAGAGCTCGG+AGG + chr8.4:41900685-41900704 None:intergenic 55.0%
TGAGCCTCGTCTCTCGCAAA+TGG - chr8.4:41899323-41899342 MS.gene63224:CDS 55.0%
TGAGCTCTGCCAAAGGACCA+AGG + chr8.4:41899862-41899881 None:intergenic 55.0%
TGATGAACGAACCGGAGGAG+TGG - chr8.4:41901261-41901280 MS.gene63224:CDS 55.0%
TTCCTCAAGGTGGGGTCCAA+TGG + chr8.4:41899584-41899603 None:intergenic 55.0%
TTCGTCGCTCACGCGTCATT+CGG - chr8.4:41899479-41899498 MS.gene63224:CDS 55.0%
TTGCTTGCCTGAGGGAACCA+AGG + chr8.4:41899675-41899694 None:intergenic 55.0%
CGGCGAAAGGATCGGGATGA+TGG + chr8.4:41899531-41899550 None:intergenic 60.0%
GAACGAACCGGAGGAGTGGA+AGG - chr8.4:41901265-41901284 MS.gene63224:CDS 60.0%
TGCCATTGGACCCCACCTTG+AGG - chr8.4:41899579-41899598 MS.gene63224:CDS 60.0%
TGCGCCATTTGCGAGAGACG+AGG + chr8.4:41899330-41899349 None:intergenic 60.0%
TGGCACATCGTCGATCCCCT+TGG + chr8.4:41901308-41901327 None:intergenic 60.0%
TTCGCGATGCCGCCTCTGTT+GGG - chr8.4:41900579-41900598 MS.gene63224:intron 60.0%
TTTCGCGATGCCGCCTCTGT+TGG - chr8.4:41900578-41900597 MS.gene63224:intron 60.0%
!! CGTCCTCGTTGGTGTTGTCG+TGG + chr8.4:41899384-41899403 None:intergenic 60.0%
AGAGGCGGCATCGCGAAACG+TGG + chr8.4:41900576-41900595 None:intergenic 65.0%
CGCGTGAGCGACGAAGAACG+AGG + chr8.4:41899474-41899493 None:intergenic 65.0%
! GGCTTGGTAGCCGTCCTCGT+TGG + chr8.4:41899395-41899414 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 41899322 41901343 41899322 ID=MS.gene63224
chr8.4 mRNA 41899322 41901343 41899322 ID=MS.gene63224.t1;Parent=MS.gene63224
chr8.4 exon 41900958 41901343 41900958 ID=MS.gene63224.t1.exon1;Parent=MS.gene63224.t1
chr8.4 CDS 41900958 41901343 41900958 ID=cds.MS.gene63224.t1;Parent=MS.gene63224.t1
chr8.4 exon 41899929 41900382 41899929 ID=MS.gene63224.t1.exon2;Parent=MS.gene63224.t1
chr8.4 CDS 41899929 41900382 41899929 ID=cds.MS.gene63224.t1;Parent=MS.gene63224.t1
chr8.4 exon 41899683 41899832 41899683 ID=MS.gene63224.t1.exon3;Parent=MS.gene63224.t1
chr8.4 CDS 41899683 41899832 41899683 ID=cds.MS.gene63224.t1;Parent=MS.gene63224.t1
chr8.4 exon 41899322 41899600 41899322 ID=MS.gene63224.t1.exon4;Parent=MS.gene63224.t1
chr8.4 CDS 41899322 41899600 41899322 ID=cds.MS.gene63224.t1;Parent=MS.gene63224.t1
Gene Sequence

>MS.gene63224

ATGAGCCTCGTCTCTCGCAAATGGCGCAACCTTTGGAAGAATCTCGATGTCTTCGATTTCCACGACAACACCAACGAGGACGGCTACCAAGCCCCCGAAGACGTATTCTACGAACAATTCATGCTCTTTACCGTTTTCGTCAACACTGTCCTCGTTCTTCGTCGCTCACGCGTCATTCGGAAGTTCCGTCTCTCATGCGATCACATCCATCATCCCGATCCTTTCGCCGATCACTCAATTGCCACGTGGATCAGTACTGCCATTGGACCCCACCTTGAGGAATTTCATCTTACTCTCTTTTCTGCCGCTGGCTTCAACAACCTGCCTCTCACACTCTTCTCTTGCTCTAACCTTGGTTCCCTCAGGCAAGCAACAATTCCTTTCACGTTAGGCTTAAATATTGCGAATGTTCCATACAAATATCAAACATTTTGGTTTTAGTATTTATAATTGTTTTTTTTCTTCAGGTTTTAGTGCTTGCAAATATATAAGTTTTTGCTTTTAGTCCTTGCAAAATTGATTCATATTGGATTTGATCCTTGGTCCTTTGGCAGAGCTCATTAATAATCACTTGAGCTAAATTGCTTGGTTTGATTCATATTGGATTTGGTCCTTGTAATTTGTAAAATATTTGATTGAAGTCCTTCAAAATAAGGACTAAAATGAATACTATAGTACCAATTTCAATATGAGTAGATTTTACAAGGACTAAAACAAAAGACTCTATATTTGCAAGGACTAAAACCAAAAAAATATTTTTAAAGAGACTAAAACCAAAAGGCTTGATATTTACAAGGATCATTTGCATATTTAAGCCTAATTGTTTAGTACTTTCTTTTTCTACAAATTATACGAGTGCTTGCTTTTAATCCCTAAACAACAGATACTAAACATATTTAACCACAATTATTTTACTTAGTTCAGCAGCTATTCTGATTTAATTTCATGTAATGTACCTCAGTCTCAATGGCTATATCTTGTTGCAATTGCAAGACACTTCGGAGGTTTGCTTACCGTCATTGAAGGTGCTACAACTACTAGATATGTATCACTTGGATTTGAATTCAGTGAATACCCTTCTCTCGAGCTGTCCAATTCTTGAAAATCTGGAACTTTCCTTTCGTCCTGAATCTTTGGCCATAATACGGGTGCCATCTTCTTTGAAGAGGTTAAAAATCACTGTTGAGAATATGGTTGGGGTTTGGCTTGAAATAGATGCACCTAGTCTCAAGTACCTTAGCCTCGAACATACCACGTTTCGCGATGCCGCCTCTGTTGGGAACTTGTACAACGTGGAAGAAGCATTTCTCGATGTACTTCCCACTCCTGAAAGTGAATCTGTTGAACCTTTACTCAATCTCCTCCGAGCTCTCTCCGGAATTAAACATCTCAAGCTGTTTAGTTCAACAACAAAGGTGTCGTAAGTCTTGATTATTTGTATTGTATGTAGTTTTTTTACTAGTAACTATAAAATAGTCAGTTAATCACTTGATGCTGTCGTGTACTTGCAGTGGCTATCTGCTGCCCCAACTTTAGATTTTCCAGAATTTCGTTATCTGCTTCCCCTAGAGCTCTTTCTTCTCTCTTTCAACTTTAATTTTATATTCAACATGCTTCATAAATGTCCTATCCTGCAAACTCTGATAACATTCAACGACAAGGTATGTTCAGTTTAACTAAAGCTTTGATGTGTGTGTGTGTGTTTTGTTTGAATAATTTATGTGAGTTGCCCCTTGTTGCCAGATGAATCCATCGTATGATTCATCACCTGCATATGGATGGAAGATGAAGCCTCAAAGTGTTCCTAAGTGTCTCATATCCCATTTGACCTTTATTAAATTTGAAGCATATCTAGGACATTTAAATGAGCTGGAATTTATTGGCTATGTTTTGCAAAATGGACTTGTTTTGAAGACAGTGCTTATTGATGAATTCTATATGATGAACGAACCGGAGGAGTGGAAGGAAAAAATTTCTGATCTGCCAAGGGGATCGACGATGTGCCAACTTAAATTTCATTGA
Protein sequence

>MS.gene63224.t1

MSLVSRKWRNLWKNLDVFDFHDNTNEDGYQAPEDVFYEQFMLFTVFVNTVLVLRRSRVIRKFRLSCDHIHHPDPFADHSIATWISTAIGPHLEEFHLTLFSAAGFNNLPLTLFSCSNLGSLRQATIPFTLNGYILLQLQDTSEVCLPSLKVLQLLDMYHLDLNSVNTLLSSCPILENLELSFRPESLAIIRVPSSLKRLKITVENMVGVWLEIDAPSLKYLSLEHTTFRDAASVGNLYNVEEAFLDVLPTPESESVEPLLNLLRALSGIKHLKLFSSTTKWLSAAPTLDFPEFRYLLPLELFLLSFNFNFIFNMLHKCPILQTLITFNDKMNPSYDSSPAYGWKMKPQSVPKCLISHLTFIKFEAYLGHLNELEFIGYVLQNGLVLKTVLIDEFYMMNEPEEWKEKISDLPRGSTMCQLKFH