AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053313

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053313.t1 MTR_8g062270 88.889 414 21 1 1 389 41 454 0.0 740
MS.gene053313.t1 MTR_8g468760 61.722 418 97 8 1 388 34 418 7.97e-162 461
MS.gene053313.t1 MTR_5g092960 57.720 421 137 7 3 388 47 461 1.03e-150 434
MS.gene053313.t1 MTR_8g468840 57.452 416 140 8 2 387 40 448 3.88e-147 424
MS.gene053313.t1 MTR_8g467800 51.435 418 161 8 2 388 46 452 5.65e-126 371
MS.gene053313.t1 MTR_2g083840 48.578 422 171 7 1 388 47 456 6.29e-117 348
MS.gene053313.t1 MTR_4g032730 47.113 433 173 10 3 388 43 466 1.36e-112 337
MS.gene053313.t1 MTR_5g092960 57.669 326 102 5 3 295 47 369 1.68e-110 329
MS.gene053313.t1 MTR_8g468810 45.561 428 177 12 1 388 51 462 2.96e-107 323
MS.gene053313.t1 MTR_8g058970 45.964 446 169 13 1 388 45 476 4.20e-103 313
MS.gene053313.t1 MTR_8g468810 46.914 324 132 8 1 292 51 366 3.20e-80 251
MS.gene053313.t1 MTR_8g445780 42.936 361 154 9 4 335 41 378 4.29e-74 236
MS.gene053313.t1 MTR_8g014260 39.529 425 199 15 4 388 44 450 4.97e-74 238
MS.gene053313.t1 MTR_8g058965 39.907 431 200 12 2 388 40 455 3.65e-72 233
MS.gene053313.t1 MTR_8g469020 36.490 433 213 10 2 388 34 450 5.43e-69 224
MS.gene053313.t1 MTR_8g468810 48.106 264 105 5 1 239 51 307 1.25e-66 214
MS.gene053313.t1 MTR_8g468810 48.106 264 105 5 1 239 51 307 1.31e-66 214
MS.gene053313.t1 MTR_8g468810 48.106 264 105 5 1 239 51 307 2.81e-66 214
MS.gene053313.t1 MTR_8g070950 38.242 421 199 14 1 388 41 433 2.96e-65 214
MS.gene053313.t1 MTR_8g062010 37.321 418 209 13 1 388 35 429 1.47e-63 209
MS.gene053313.t1 MTR_8g062310 37.441 422 179 17 4 388 54 427 7.98e-63 207
MS.gene053313.t1 MTR_8g467800 47.273 275 105 7 2 247 46 309 3.05e-61 201
MS.gene053313.t1 MTR_4g050250 39.788 377 177 9 1 335 47 415 1.76e-59 199
MS.gene053313.t1 MTR_8g062080 36.150 426 204 15 1 388 49 444 2.16e-58 196
MS.gene053313.t1 MTR_8g014210 46.667 240 113 7 154 388 1 230 3.81e-54 179
MS.gene053313.t1 MTR_8g062300 33.843 458 200 19 1 388 52 476 4.88e-52 181
MS.gene053313.t1 MTR_4g024710 39.777 269 146 7 126 388 88 346 4.99e-47 164
MS.gene053313.t1 MTR_8g077460 32.000 400 194 16 4 388 38 374 1.29e-43 155
MS.gene053313.t1 MTR_4g024745 38.434 281 158 7 95 370 10 280 5.75e-39 141
MS.gene053313.t1 MTR_8g014160 37.545 277 137 9 2 247 67 338 1.01e-37 141
MS.gene053313.t1 MTR_8g467790 31.956 363 169 12 1 333 174 488 1.46e-36 139
MS.gene053313.t1 MTR_5g080710 28.979 421 225 18 2 381 59 446 7.63e-25 105
MS.gene053313.t1 MTR_5g084070 29.298 413 227 19 2 381 39 419 3.17e-24 103
MS.gene053313.t1 MTR_2g086020 41.892 148 72 5 247 388 175 314 3.23e-21 93.6
MS.gene053313.t1 MTR_2g086020 37.903 124 57 5 1 118 1 110 4.21e-11 63.9
MS.gene053313.t1 MTR_8g014290 44.628 121 60 5 4 118 38 157 5.22e-21 89.4
MS.gene053313.t1 MTR_5g021550 45.833 120 54 5 4 118 47 160 1.44e-20 88.2
MS.gene053313.t1 MTR_4g080787 27.924 419 241 22 2 376 45 446 6.91e-15 76.3
MS.gene053313.t1 MTR_4g024720 37.226 137 57 5 4 118 45 174 1.23e-13 69.7
MS.gene053313.t1 MTR_4g113810 31.544 149 75 4 78 199 18 166 3.53e-11 61.6
MS.gene053313.t1 MTR_5g039950 27.273 374 217 15 2 357 49 385 8.38e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053313.t1 AT5G53840 24.941 421 253 17 2 386 44 437 7.65e-23 100
MS.gene053313.t1 AT1G16930 24.943 437 245 18 2 386 41 446 1.26e-16 81.6
MS.gene053313.t1 AT1G16930 24.943 437 245 18 2 386 41 446 1.26e-16 81.6
MS.gene053313.t1 AT1G16930 24.943 437 245 18 2 386 41 446 1.26e-16 81.6
MS.gene053313.t1 AT5G53635 24.709 429 252 16 3 389 27 426 2.33e-15 77.8
MS.gene053313.t1 AT1G13780 25.113 442 249 18 2 386 36 452 3.98e-15 77.0
MS.gene053313.t1 AT5G38570 26.124 356 211 16 46 388 59 375 6.10e-15 76.3
MS.gene053313.t1 AT4G00160 29.639 388 202 22 2 351 42 396 7.58e-15 76.3
MS.gene053313.t1 AT1G78750 24.036 441 256 18 2 389 44 458 3.16e-14 74.3
MS.gene053313.t1 AT1G78750 24.036 441 256 18 2 389 44 458 3.16e-14 74.3
MS.gene053313.t1 AT1G51370 23.389 419 256 14 2 386 45 432 8.14e-14 73.2
MS.gene053313.t1 AT5G22660 25.191 393 216 18 56 389 73 446 1.12e-13 72.8
MS.gene053313.t1 AT5G56420 24.057 424 251 17 2 388 32 421 1.20e-13 72.4
MS.gene053313.t1 AT5G56420 24.057 424 251 17 2 388 32 421 1.20e-13 72.4
MS.gene053313.t1 AT5G56420 24.057 424 251 17 2 388 32 421 1.20e-13 72.4
MS.gene053313.t1 AT5G27750 25.301 415 232 18 2 359 31 424 2.25e-13 71.6
MS.gene053313.t1 AT3G26920 27.246 345 193 16 83 389 16 340 2.82e-13 70.9
MS.gene053313.t1 AT3G26920 27.246 345 193 16 83 389 16 340 2.82e-13 70.9
MS.gene053313.t1 AT1G69630 22.624 442 254 16 2 389 44 451 3.43e-13 71.2
MS.gene053313.t1 AT5G56820 26.744 430 223 22 2 386 41 423 7.53e-13 70.1
MS.gene053313.t1 AT5G60610 25.954 393 216 18 2 356 28 383 1.23e-12 69.3
MS.gene053313.t1 AT5G60610 25.954 393 216 18 2 356 28 383 1.23e-12 69.3
MS.gene053313.t1 AT1G06630 24.702 336 206 10 2 300 38 363 1.73e-12 68.9
MS.gene053313.t1 AT1G06630 24.702 336 206 10 2 300 38 363 1.73e-12 68.9
MS.gene053313.t1 AT1G06630 24.702 336 206 10 2 300 38 363 1.73e-12 68.9
MS.gene053313.t1 AT1G06630 24.702 336 206 10 2 300 38 363 1.73e-12 68.9
MS.gene053313.t1 AT3G52680 28.788 396 201 22 2 355 47 403 1.79e-12 68.9
MS.gene053313.t1 AT1G06630 25.077 323 200 9 2 292 38 350 1.98e-12 68.2
MS.gene053313.t1 AT1G06630 25.077 323 200 9 2 292 38 350 1.98e-12 68.2
MS.gene053313.t1 AT1G06630 24.702 336 206 10 2 300 47 372 2.09e-12 68.6
MS.gene053313.t1 AT3G50710 24.769 432 241 20 3 386 29 424 2.44e-12 68.6
MS.gene053313.t1 AT5G22730 23.025 443 250 17 2 386 54 463 3.50e-12 68.2
MS.gene053313.t1 AT5G22730 23.025 443 250 17 2 386 54 463 3.50e-12 68.2
MS.gene053313.t1 AT5G22720 23.326 463 234 21 2 389 49 465 3.51e-12 68.2
MS.gene053313.t1 AT1G06630 24.702 336 206 10 2 300 38 363 3.81e-12 68.2
MS.gene053313.t1 AT4G26340 23.005 426 254 16 2 388 28 418 5.81e-12 67.4
MS.gene053313.t1 AT2G26030 22.955 440 258 19 2 386 30 443 2.05e-11 65.9
MS.gene053313.t1 AT1G78760 22.274 431 257 19 2 386 42 440 3.21e-11 65.1
MS.gene053313.t1 AT2G26030 22.955 440 253 19 2 386 30 438 3.34e-11 65.1
MS.gene053313.t1 AT2G26030 22.955 440 253 19 2 386 30 438 3.34e-11 65.1
MS.gene053313.t1 AT1G13780 27.213 305 160 14 2 257 36 327 5.26e-11 63.9
MS.gene053313.t1 AT4G10420 24.189 339 206 13 82 388 2 321 5.98e-11 63.5
MS.gene053313.t1 AT4G10400 25.381 394 215 21 2 359 28 378 7.92e-11 63.5
MS.gene053313.t1 AT4G10400 25.381 394 215 21 2 359 28 378 7.92e-11 63.5
MS.gene053313.t1 AT4G10400 25.381 394 215 21 2 359 28 378 7.92e-11 63.5

Find 93 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTTTAGTCCTGAATCTT+TGG 0.225367 8.3:-39426452 MS.gene053313:CDS
GAAAATGAGCTGGAATTTAT+TGG 0.228561 8.3:-39425704 MS.gene053313:CDS
AAACTCCGGAAAATGTAAAA+TGG 0.241590 8.3:+39426040 None:intergenic
CTGAATCTTTGGACATAATT+CGG 0.268479 8.3:-39426441 MS.gene053313:CDS
CCAGAGGTTGCGCCATTTGC+GGG 0.271885 8.3:+39427242 None:intergenic
ATTCGTTGCCTTCATATAGA+TGG 0.301405 8.3:-39425799 MS.gene053313:CDS
AAATGGGATATGAGGCATTT+AGG 0.311419 8.3:+39425755 None:intergenic
CGAATTATGTCCAAAGATTC+AGG 0.318966 8.3:+39426442 None:intergenic
CAATGATTGTTGATGAATAT+TGG 0.328994 8.3:-39425649 MS.gene053313:CDS
AATCTCCTTCGAGCTCTTTC+CGG 0.331425 8.3:-39426211 MS.gene053313:CDS
TTCCGTGATGCCGCGGCTGT+TGG 0.352559 8.3:-39426310 MS.gene053313:CDS
AGAGGGTGAGATTAAATTCC+TGG 0.363398 8.3:+39426983 None:intergenic
AAAATGAGCTGGAATTTATT+GGG 0.368042 8.3:-39425703 MS.gene053313:CDS
CTCAAGTACCTTAGCCTTGA+AGG 0.369562 8.3:-39426340 MS.gene053313:CDS
TCCGTGATGCCGCGGCTGTT+GGG 0.374852 8.3:-39426309 MS.gene053313:CDS
TCCGTCGCTCACGCGTCGTT+CGG 0.384887 8.3:-39427098 MS.gene053313:CDS
AAAAGTGAATAAAGGTCAAA+TGG 0.385833 8.3:+39425738 None:intergenic
CCCGCAAATGGCGCAACCTC+TGG 0.386923 8.3:-39427242 MS.gene053313:CDS
TGAATCTTTGGACATAATTC+GGG 0.390671 8.3:-39426440 MS.gene053313:CDS
CTGTCCTGTTCTCGAGAATC+TGG 0.392606 8.3:-39426479 MS.gene053313:CDS
TAGTTCTAGACGTTTAATTC+CGG 0.398389 8.3:+39426192 None:intergenic
ATCACTGTTGATAATAATGT+TGG 0.404587 8.3:-39426391 MS.gene053313:CDS
AACTCCGGAAAATGTAAAAT+GGG 0.406590 8.3:+39426041 None:intergenic
CGCCATTGGGCCCCACCTCC+AGG 0.411986 8.3:-39427001 MS.gene053313:CDS
TCCAGAGGTTGCGCCATTTG+CGG 0.418266 8.3:+39427241 None:intergenic
TCACTGTTGATAATAATGTT+GGG 0.420825 8.3:-39426390 MS.gene053313:CDS
AAGAGAAGAGTGTGAGAGGT+AGG 0.423108 8.3:+39426938 None:intergenic
AAGCAAAGAATGGATCGTTT+AGG 0.433146 8.3:+39427049 None:intergenic
TTTAACCTCTTCAAGGAAGA+TGG 0.437384 8.3:+39426415 None:intergenic
TGTTCGTTGAATTCGTCATC+GGG 0.437656 8.3:+39427162 None:intergenic
GAAAACTTCAGTCACCTTTG+TGG 0.437723 8.3:+39426159 None:intergenic
AATCTCACCCTCTTTACTGC+TGG 0.442889 8.3:-39426973 MS.gene053313:CDS
CACGGAATGTGATCCCTTCA+AGG 0.450691 8.3:+39426326 None:intergenic
AGTTCCAGATTCTCGAGAAC+AGG 0.463808 8.3:+39426475 None:intergenic
TAAAGGTCAAATGGGATATG+AGG 0.466574 8.3:+39425747 None:intergenic
TTGATAATAATGTTGGGGCT+TGG 0.473819 8.3:-39426384 MS.gene053313:CDS
TGAGCCTAGTCTCCCGCAAA+TGG 0.477237 8.3:-39427254 MS.gene053313:CDS
TTGTTCGTTGAATTCGTCAT+CGG 0.482869 8.3:+39427161 None:intergenic
CGTGGCTCAGTATCGCCATT+GGG 0.488486 8.3:-39427014 MS.gene053313:CDS
GCTATGAGAGTTTCTAGCAT+TGG 0.489509 8.3:+39425942 None:intergenic
GAGCAAGAGAAGAGTGTGAG+AGG 0.493002 8.3:+39426934 None:intergenic
TTCGTTGCCTTCATATAGAT+GGG 0.495093 8.3:-39425798 MS.gene053313:CDS
ACATCCGTAAAAGTGAATAA+AGG 0.501125 8.3:+39425730 None:intergenic
TTCCTGGAGGTGGGGCCCAA+TGG 0.507405 8.3:+39426999 None:intergenic
GAGATTAAATTCCTGGAGGT+GGG 0.510070 8.3:+39426990 None:intergenic
AAAGTGAATAAAGGTCAAAT+GGG 0.512234 8.3:+39425739 None:intergenic
TGTGAGAGGTAGGTTGTTGA+AGG 0.512486 8.3:+39426948 None:intergenic
GAGAACAGTGTTGACGAAAA+CGG 0.512616 8.3:+39427125 None:intergenic
TGCGCCATTTGCGGGAGACT+AGG 0.515202 8.3:+39427250 None:intergenic
TGAGATTAAATTCCTGGAGG+TGG 0.517495 8.3:+39426989 None:intergenic
TGGCACATGGCAGATCCTCT+TGG 0.523168 8.3:+39425584 None:intergenic
CACTGTTGATAATAATGTTG+GGG 0.535843 8.3:-39426389 MS.gene053313:CDS
TCGACTGAGTAAGCAAAGAA+TGG 0.539936 8.3:+39427039 None:intergenic
CAGATTCTCGAGAACAGGAC+AGG 0.544753 8.3:+39426480 None:intergenic
GGCGAGACCAACCAGACCGC+TGG 0.544821 8.3:-39425628 MS.gene053313:CDS
ACGTGGCTCAGTATCGCCAT+TGG 0.547510 8.3:-39427015 MS.gene053313:CDS
TCCGAACGACGCGTGAGCGA+CGG 0.553880 8.3:+39427097 None:intergenic
TTTGCCTCCCATCTATATGA+AGG 0.559497 8.3:+39425791 None:intergenic
TGGCTTGAAATAGATGCAGC+TGG 0.559523 8.3:-39426364 MS.gene053313:CDS
ATGTGATCCCTTCAAGGCTA+AGG 0.560362 8.3:+39426332 None:intergenic
TCAGTCAGAGAAATTTAACT+TGG 0.568365 8.3:+39425564 None:intergenic
TGAAGGCACCAGCAGTAAAG+AGG 0.569444 8.3:+39426965 None:intergenic
TGAAGCAAATAGTGAAACTC+CGG 0.573562 8.3:+39426026 None:intergenic
CGGATGTCCAGAAAATGAGC+TGG 0.574811 8.3:-39425714 MS.gene053313:CDS
AGAATGGATCGTTTAGGATG+TGG 0.575368 8.3:+39427055 None:intergenic
TCCCAACAGCCGCGGCATCA+CGG 0.577725 8.3:+39426308 None:intergenic
GGGTGCCATCTTCCTTGAAG+AGG 0.580167 8.3:-39426420 MS.gene053313:CDS
GAACAGGACAGGCAGAGAGA+AGG 0.583613 8.3:+39426491 None:intergenic
TCAAGTACCTTAGCCTTGAA+GGG 0.585522 8.3:-39426339 MS.gene053313:CDS
GGATGTGGTCGGAGGAGAGA+CGG 0.588995 8.3:+39427070 None:intergenic
AGATTAAATTCCTGGAGGTG+GGG 0.594581 8.3:+39426991 None:intergenic
TTCGTTGAATTCGTCATCGG+GGG 0.595692 8.3:+39427164 None:intergenic
AGACTTCGAGATTCTTCCAG+AGG 0.596831 8.3:+39427226 None:intergenic
GAAGGCACCAGCAGTAAAGA+GGG 0.604956 8.3:+39426966 None:intergenic
GATCACATTCCGTGATGCCG+CGG 0.609193 8.3:-39426317 MS.gene053313:CDS
TTGCTTACTCAGTCGACACG+TGG 0.615356 8.3:-39427032 MS.gene053313:CDS
GCTCGAAGGAGATTGAGTAA+AGG 0.617059 8.3:+39426220 None:intergenic
GGCAGAGAGAAGGATATGCA+TGG 0.624602 8.3:+39426501 None:intergenic
ATTTAGTTTCGACATCGACA+TGG 0.626202 8.3:-39425919 MS.gene053313:intron
GTGCAAGTTCCCAACAGCCG+CGG 0.628805 8.3:+39426300 None:intergenic
ACTCCGGAAAATGTAAAATG+GGG 0.632256 8.3:+39426042 None:intergenic
AAAATTTCAGATCTACCAAG+AGG 0.633493 8.3:-39425599 MS.gene053313:CDS
GAGAAATTTAACTTGGCACA+TGG 0.635924 8.3:+39425571 None:intergenic
TGTATTCATTGCATTTGTCG+CGG 0.641710 8.3:+39427196 None:intergenic
ATCGTTTAGGATGTGGTCGG+AGG 0.648456 8.3:+39427062 None:intergenic
GTTCGTTGAATTCGTCATCG+GGG 0.649274 8.3:+39427163 None:intergenic
CTCCGGAAAATGTAAAATGG+GGG 0.656185 8.3:+39426043 None:intergenic
TGGATCGTTTAGGATGTGGT+CGG 0.656581 8.3:+39427059 None:intergenic
ACTACATAATTCAACCACAA+AGG 0.657271 8.3:-39426173 MS.gene053313:intron
TAATTCCGGAAAGAGCTCGA+AGG 0.661187 8.3:+39426206 None:intergenic
GTTGCCTTCATATAGATGGG+AGG 0.669069 8.3:-39425795 MS.gene053313:CDS
TGTTGGGAACTTGCACAGCG+TGG 0.697966 8.3:-39426293 MS.gene053313:CDS
GGGTGAGATTAAATTCCTGG+AGG 0.740357 8.3:+39426986 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACAAAAATAAAGAAAAG+AGG + chr8.3:39425956-39425975 None:intergenic 15.0%
!! GCTATATATAAGAGAAAATT+GGG + chr8.3:39426073-39426092 None:intergenic 20.0%
!! TGCTATATATAAGAGAAAAT+TGG + chr8.3:39426074-39426093 None:intergenic 20.0%
! AAAAGTGAATAAAGGTCAAA+TGG + chr8.3:39427088-39427107 None:intergenic 25.0%
! AAAATGAGCTGGAATTTATT+GGG - chr8.3:39427120-39427139 MS.gene053313:CDS 25.0%
! AAAGTGAATAAAGGTCAAAT+GGG + chr8.3:39427087-39427106 None:intergenic 25.0%
! TAAAGAAAAGAGGATGAAAT+AGG + chr8.3:39425946-39425965 None:intergenic 25.0%
!! CAATGATTGTTGATGAATAT+TGG - chr8.3:39427174-39427193 MS.gene053313:CDS 25.0%
!! TTTTCTCTTATATATAGCAC+CGG - chr8.3:39426075-39426094 MS.gene053313:intron 25.0%
!!! AACAAAAAAGTTGAGTTGAA+AGG + chr8.3:39426842-39426861 None:intergenic 25.0%
!!! ACAAAAAAGTTGAGTTGAAA+GGG + chr8.3:39426841-39426860 None:intergenic 25.0%
!!! ATCACTGTTGATAATAATGT+TGG - chr8.3:39426432-39426451 MS.gene053313:CDS 25.0%
!!! TCACTGTTGATAATAATGTT+GGG - chr8.3:39426433-39426452 MS.gene053313:CDS 25.0%
!!! TTGACCTTTATTCACTTTTA+CGG - chr8.3:39427089-39427108 MS.gene053313:CDS 25.0%
AAAATTTCAGATCTACCAAG+AGG - chr8.3:39427224-39427243 MS.gene053313:CDS 30.0%
AAACTCCGGAAAATGTAAAA+TGG + chr8.3:39426786-39426805 None:intergenic 30.0%
AACTCCGGAAAATGTAAAAT+GGG + chr8.3:39426785-39426804 None:intergenic 30.0%
ACATCCGTAAAAGTGAATAA+AGG + chr8.3:39427096-39427115 None:intergenic 30.0%
ACTACATAATTCAACCACAA+AGG - chr8.3:39426650-39426669 MS.gene053313:intron 30.0%
CTGAATCTTTGGACATAATT+CGG - chr8.3:39426382-39426401 MS.gene053313:CDS 30.0%
GAAAATGAGCTGGAATTTAT+TGG - chr8.3:39427119-39427138 MS.gene053313:CDS 30.0%
TAAAGTTAAACTACTTCCTC+CGG + chr8.3:39426097-39426116 None:intergenic 30.0%
TGAATCTTTGGACATAATTC+GGG - chr8.3:39426383-39426402 MS.gene053313:CDS 30.0%
TTTCTTTAGTCCTGAATCTT+TGG - chr8.3:39426371-39426390 MS.gene053313:CDS 30.0%
! AGTGATTTTTAACCTCTTCA+AGG + chr8.3:39426418-39426437 None:intergenic 30.0%
! ATAAATTCCAGCTCATTTTC+TGG + chr8.3:39427119-39427138 None:intergenic 30.0%
! ATTGGGTATATTTTGCAACA+TGG - chr8.3:39427137-39427156 MS.gene053313:CDS 30.0%
! CATTCCATTGTTATTGTCTA+GGG - chr8.3:39425979-39425998 MS.gene053313:CDS 30.0%
!! AAAAAAGTTGAGTTGAAAGG+GGG + chr8.3:39426839-39426858 None:intergenic 30.0%
!! AATTGGAAAGTTGATGTATC+CGG - chr8.3:39426010-39426029 MS.gene053313:CDS 30.0%
!! CAAAAAAGTTGAGTTGAAAG+GGG + chr8.3:39426840-39426859 None:intergenic 30.0%
!! CACTGTTGATAATAATGTTG+GGG - chr8.3:39426434-39426453 MS.gene053313:CDS 30.0%
!! TAGTTCTAGACGTTTAATTC+CGG + chr8.3:39426634-39426653 None:intergenic 30.0%
!!! CTGAAATTTTTTTCATCCAG+CGG + chr8.3:39427214-39427233 None:intergenic 30.0%
!!! GTGTTCGTGTTTTTTTTGTT+TGG - chr8.3:39426954-39426973 MS.gene053313:CDS 30.0%
AAGCAAAGAATGGATCGTTT+AGG + chr8.3:39425777-39425796 None:intergenic 35.0%
ACTCCGGAAAATGTAAAATG+GGG + chr8.3:39426784-39426803 None:intergenic 35.0%
ATCCGGTTCAAAATTTGGAT+CGG - chr8.3:39426027-39426046 MS.gene053313:CDS 35.0%
ATGTAATGTAACAGTCTCAG+TGG - chr8.3:39426204-39426223 MS.gene053313:CDS 35.0%
ATTCGTTGCCTTCATATAGA+TGG - chr8.3:39427024-39427043 MS.gene053313:CDS 35.0%
CGAATTATGTCCAAAGATTC+AGG + chr8.3:39426384-39426403 None:intergenic 35.0%
CTAACCCTAGACAATAACAA+TGG + chr8.3:39425986-39426005 None:intergenic 35.0%
GATGTATCCGGTTCAAAATT+TGG - chr8.3:39426022-39426041 MS.gene053313:CDS 35.0%
TAAAGGTCAAATGGGATATG+AGG + chr8.3:39427079-39427098 None:intergenic 35.0%
TGAAGCAAATAGTGAAACTC+CGG + chr8.3:39426800-39426819 None:intergenic 35.0%
TGTATTCATTGCATTTGTCG+CGG + chr8.3:39425630-39425649 None:intergenic 35.0%
TTCGTTGCCTTCATATAGAT+GGG - chr8.3:39427025-39427044 MS.gene053313:CDS 35.0%
TTGTTCGTTGAATTCGTCAT+CGG + chr8.3:39425665-39425684 None:intergenic 35.0%
TTTAACCTCTTCAAGGAAGA+TGG + chr8.3:39426411-39426430 None:intergenic 35.0%
! AAATGGGATATGAGGCATTT+AGG + chr8.3:39427071-39427090 None:intergenic 35.0%
! ATCCGATCCAAATTTTGAAC+CGG + chr8.3:39426032-39426051 None:intergenic 35.0%
! ATTTAGTTTCGACATCGACA+TGG - chr8.3:39426904-39426923 MS.gene053313:intron 35.0%
! GCATTCCATTGTTATTGTCT+AGG - chr8.3:39425978-39425997 MS.gene053313:CDS 35.0%
! TGTCTAGGGTTAGAGAAAAT+TGG - chr8.3:39425993-39426012 MS.gene053313:CDS 35.0%
! TTGATAATAATGTTGGGGCT+TGG - chr8.3:39426439-39426458 MS.gene053313:CDS 35.0%
!! ATCAGATTCACTTTTAGGAG+TGG + chr8.3:39426580-39426599 None:intergenic 35.0%
!! GAGAAATTTAACTTGGCACA+TGG + chr8.3:39427255-39427274 None:intergenic 35.0%
!! TCAGATTCACTTTTAGGAGT+GGG + chr8.3:39426579-39426598 None:intergenic 35.0%
AAATAGCCACTGCTTGTACA+CGG + chr8.3:39426756-39426775 None:intergenic 40.0%
AATCAAATGATCGATCCACC+TGG + chr8.3:39427007-39427026 None:intergenic 40.0%
ACGGTAAGCAAATTTCCGAA+GGG + chr8.3:39426254-39426273 None:intergenic 40.0%
AGAGGGTGAGATTAAATTCC+TGG + chr8.3:39425843-39425862 None:intergenic 40.0%
AGATTAAATTCCTGGAGGTG+GGG + chr8.3:39425835-39425854 None:intergenic 40.0%
CAACAAAGCTCGAGTTAAAC+TGG + chr8.3:39426935-39426954 None:intergenic 40.0%
CTCCGGAAAATGTAAAATGG+GGG + chr8.3:39426783-39426802 None:intergenic 40.0%
GAAAACTTCAGTCACCTTTG+TGG + chr8.3:39426667-39426686 None:intergenic 40.0%
GAGATTAAATTCCTGGAGGT+GGG + chr8.3:39425836-39425855 None:intergenic 40.0%
TCGACTGAGTAAGCAAAGAA+TGG + chr8.3:39425787-39425806 None:intergenic 40.0%
TGAGATTAAATTCCTGGAGG+TGG + chr8.3:39425837-39425856 None:intergenic 40.0%
TGTTCGTTGAATTCGTCATC+GGG + chr8.3:39425664-39425683 None:intergenic 40.0%
TTTGCCTCCCATCTATATGA+AGG + chr8.3:39427035-39427054 None:intergenic 40.0%
! AATTTGCTTACCGTCGTTGA+AGG - chr8.3:39426260-39426279 MS.gene053313:CDS 40.0%
! AGAATGGATCGTTTAGGATG+TGG + chr8.3:39425771-39425790 None:intergenic 40.0%
! TCTCTTATATATAGCACCGG+AGG - chr8.3:39426078-39426097 MS.gene053313:intron 40.0%
! TGCCCCCATTTTACATTTTC+CGG - chr8.3:39426778-39426797 MS.gene053313:intron 40.0%
!! GAGAACAGTGTTGACGAAAA+CGG + chr8.3:39425701-39425720 None:intergenic 40.0%
!! GCTATGAGAGTTTCTAGCAT+TGG + chr8.3:39426884-39426903 None:intergenic 40.0%
!! GGCTCATCAGATTCACTTTT+AGG + chr8.3:39426585-39426604 None:intergenic 40.0%
!! TCAAGTACCTTAGCCTTGAA+GGG - chr8.3:39426484-39426503 MS.gene053313:CDS 40.0%
!!! ATTTTTTTCATCCAGCGGTC+TGG + chr8.3:39427209-39427228 None:intergenic 40.0%
AATCTCACCCTCTTTACTGC+TGG - chr8.3:39425850-39425869 MS.gene053313:intron 45.0%
AATCTCCTTCGAGCTCTTTC+CGG - chr8.3:39426612-39426631 MS.gene053313:intron 45.0%
AGACTTCGAGATTCTTCCAG+AGG + chr8.3:39425600-39425619 None:intergenic 45.0%
AGTTCCAGATTCTCGAGAAC+AGG + chr8.3:39426351-39426370 None:intergenic 45.0%
ATGTGATCCCTTCAAGGCTA+AGG + chr8.3:39426494-39426513 None:intergenic 45.0%
ATTTCCGAAGGGTCTTGCAA+TGG + chr8.3:39426243-39426262 None:intergenic 45.0%
GACGGTAAGCAAATTTCCGA+AGG + chr8.3:39426255-39426274 None:intergenic 45.0%
GCTCGAAGGAGATTGAGTAA+AGG + chr8.3:39426606-39426625 None:intergenic 45.0%
GGGTGAGATTAAATTCCTGG+AGG + chr8.3:39425840-39425859 None:intergenic 45.0%
GTTCGTTGAATTCGTCATCG+GGG + chr8.3:39425663-39425682 None:intergenic 45.0%
GTTGCCTTCATATAGATGGG+AGG - chr8.3:39427028-39427047 MS.gene053313:CDS 45.0%
TAATTCCGGAAAGAGCTCGA+AGG + chr8.3:39426620-39426639 None:intergenic 45.0%
TGGCTTGAAATAGATGCAGC+TGG - chr8.3:39426459-39426478 MS.gene053313:CDS 45.0%
TGTGAGAGGTAGGTTGTTGA+AGG + chr8.3:39425878-39425897 None:intergenic 45.0%
TTCGTTGAATTCGTCATCGG+GGG + chr8.3:39425662-39425681 None:intergenic 45.0%
! AAGAGAAGAGTGTGAGAGGT+AGG + chr8.3:39425888-39425907 None:intergenic 45.0%
! TGGATCGTTTAGGATGTGGT+CGG + chr8.3:39425767-39425786 None:intergenic 45.0%
!! CTCAAGTACCTTAGCCTTGA+AGG - chr8.3:39426483-39426502 MS.gene053313:CDS 45.0%
AGTTCCAGCACCTTCAACGA+CGG + chr8.3:39426273-39426292 None:intergenic 50.0%
ATCGATCCACCTGGCAACAA+GGG + chr8.3:39426998-39427017 None:intergenic 50.0%
CACGGAATGTGATCCCTTCA+AGG + chr8.3:39426500-39426519 None:intergenic 50.0%
CAGATTCTCGAGAACAGGAC+AGG + chr8.3:39426346-39426365 None:intergenic 50.0%
CGGATGTCCAGAAAATGAGC+TGG - chr8.3:39427109-39427128 MS.gene053313:CDS 50.0%
CTGTCCTGTTCTCGAGAATC+TGG - chr8.3:39426344-39426363 MS.gene053313:CDS 50.0%
GCTCTAACCTTGTCTCTCTC+AGG - chr8.3:39425908-39425927 MS.gene053313:intron 50.0%
GGCAGAGAGAAGGATATGCA+TGG + chr8.3:39426325-39426344 None:intergenic 50.0%
GTTGCCATTGCAAGACCCTT+CGG - chr8.3:39426236-39426255 MS.gene053313:CDS 50.0%
TTGCATGCCTGAGAGAGACA+AGG + chr8.3:39425918-39425937 None:intergenic 50.0%
TTGCTTACTCAGTCGACACG+TGG - chr8.3:39425791-39425810 MS.gene053313:CDS 50.0%
! ATCGTTTAGGATGTGGTCGG+AGG + chr8.3:39425764-39425783 None:intergenic 50.0%
! CTTTTGCCGTGTACAAGCAG+TGG - chr8.3:39426747-39426766 MS.gene053313:intron 50.0%
! GAGCAAGAGAAGAGTGTGAG+AGG + chr8.3:39425892-39425911 None:intergenic 50.0%
! TTTTCATCCAGCGGTCTGGT+TGG + chr8.3:39427205-39427224 None:intergenic 50.0%
!! GAAGGCACCAGCAGTAAAGA+GGG + chr8.3:39425860-39425879 None:intergenic 50.0%
!! TGAAGGCACCAGCAGTAAAG+AGG + chr8.3:39425861-39425880 None:intergenic 50.0%
ACGTGGCTCAGTATCGCCAT+TGG - chr8.3:39425808-39425827 MS.gene053313:CDS 55.0%
ATCAGTTGCCCCTTGTTGCC+AGG - chr8.3:39426986-39427005 MS.gene053313:CDS 55.0%
CGTGGCTCAGTATCGCCATT+GGG - chr8.3:39425809-39425828 MS.gene053313:CDS 55.0%
GAACAGGACAGGCAGAGAGA+AGG + chr8.3:39426335-39426354 None:intergenic 55.0%
GATCACATTCCGTGATGCCG+CGG - chr8.3:39426506-39426525 MS.gene053313:CDS 55.0%
GATCGATCCACCTGGCAACA+AGG + chr8.3:39426999-39427018 None:intergenic 55.0%
TCCAGAGGTTGCGCCATTTG+CGG + chr8.3:39425585-39425604 None:intergenic 55.0%
TCGATCCACCTGGCAACAAG+GGG + chr8.3:39426997-39427016 None:intergenic 55.0%
TGAATTCGTCATCGGGGGCT+TGG + chr8.3:39425657-39425676 None:intergenic 55.0%
TGAGCCTAGTCTCCCGCAAA+TGG - chr8.3:39425569-39425588 MS.gene053313:CDS 55.0%
TGGCACATGGCAGATCCTCT+TGG + chr8.3:39427242-39427261 None:intergenic 55.0%
TGTTGGGAACTTGCACAGCG+TGG - chr8.3:39426530-39426549 MS.gene053313:CDS 55.0%
! CTTACCGTCGTTGAAGGTGC+TGG - chr8.3:39426266-39426285 MS.gene053313:CDS 55.0%
! GGGTGCCATCTTCCTTGAAG+AGG - chr8.3:39426403-39426422 MS.gene053313:CDS 55.0%
AGTTGCCCCTTGTTGCCAGG+TGG - chr8.3:39426989-39427008 MS.gene053313:CDS 60.0%
CCAGAGGTTGCGCCATTTGC+GGG + chr8.3:39425584-39425603 None:intergenic 60.0%
GGATGTGGTCGGAGGAGAGA+CGG + chr8.3:39425756-39425775 None:intergenic 60.0%
GTGCAAGTTCCCAACAGCCG+CGG + chr8.3:39426526-39426545 None:intergenic 60.0%
TGCGCCATTTGCGGGAGACT+AGG + chr8.3:39425576-39425595 None:intergenic 60.0%
CCCGCAAATGGCGCAACCTC+TGG - chr8.3:39425581-39425600 MS.gene053313:CDS 65.0%
TCCCAACAGCCGCGGCATCA+CGG + chr8.3:39426518-39426537 None:intergenic 65.0%
TCCGAACGACGCGTGAGCGA+CGG + chr8.3:39425729-39425748 None:intergenic 65.0%
TCCGTCGCTCACGCGTCGTT+CGG - chr8.3:39425725-39425744 MS.gene053313:CDS 65.0%
TCCGTGATGCCGCGGCTGTT+GGG - chr8.3:39426514-39426533 MS.gene053313:CDS 65.0%
TTCCGTGATGCCGCGGCTGT+TGG - chr8.3:39426513-39426532 MS.gene053313:CDS 65.0%
TTCCTGGAGGTGGGGCCCAA+TGG + chr8.3:39425827-39425846 None:intergenic 65.0%
GGCGAGACCAACCAGACCGC+TGG - chr8.3:39427195-39427214 MS.gene053313:CDS 70.0%
CGCCATTGGGCCCCACCTCC+AGG - chr8.3:39425822-39425841 MS.gene053313:intron 75.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 39425568 39427277 39425568 ID=MS.gene053313
chr8.3 mRNA 39425568 39427277 39425568 ID=MS.gene053313.t1;Parent=MS.gene053313
chr8.3 exon 39426927 39427277 39426927 ID=MS.gene053313.t1.exon1;Parent=MS.gene053313.t1
chr8.3 CDS 39426927 39427277 39426927 ID=cds.MS.gene053313.t1;Parent=MS.gene053313.t1
chr8.3 exon 39426174 39426563 39426174 ID=MS.gene053313.t1.exon2;Parent=MS.gene053313.t1
chr8.3 CDS 39426174 39426563 39426174 ID=cds.MS.gene053313.t1;Parent=MS.gene053313.t1
chr8.3 exon 39425920 39426078 39425920 ID=MS.gene053313.t1.exon3;Parent=MS.gene053313.t1
chr8.3 CDS 39425920 39426078 39425920 ID=cds.MS.gene053313.t1;Parent=MS.gene053313.t1
chr8.3 exon 39425568 39425837 39425568 ID=MS.gene053313.t1.exon4;Parent=MS.gene053313.t1
chr8.3 CDS 39425568 39425837 39425568 ID=cds.MS.gene053313.t1;Parent=MS.gene053313.t1
Gene Sequence

>MS.gene053313

ATGAGCCTAGTCTCCCGCAAATGGCGCAACCTCTGGAAGAATCTCGAAGTCTTCGATTTCCGCGACAAATGCAATGAATACAGCTACCAAGCCCCCGATGACGAATTCAACGAACAATTCATGCTGTTCACCGTTTTCGTCAACACTGTTCTCTCTCTCCGTCGCTCACGCGTCGTTCGGAAGTTCCGTCTCTCCTCCGACCACATCCTAAACGATCCATTCTTTGCTTACTCAGTCGACACGTGGCTCAGTATCGCCATTGGGCCCCACCTCCAGGAATTTAATCTCACCCTCTTTACTGCTGGTGCCTTCAACAACCTACCTCTCACACTCTTCTCTTGCTCTAACCTTGTCTCTCTCAGGCATGCAACAATTCCTATTTCATCCTCTTTTCTTTATTTTTGTTTTCTGCATTCCATTGTTATTGTCTAGGGTTAGAGAAAATTGGAAAGTTGATGTATCCGGTTCAAAATTTGGATCGGATACATCAACTTTTTGTTAACCCAATTTTCTCTTATATATAGCACCGGAGGAAGTAGTTTAACTTTAATTTTTATGCTTGTTCAATTTAGCTGTTTTGATTTAATTTAAATGAGATATGATACATGATGATAACTTAACATAACACATTCATGAATGTAATGTAACAGTCTCAGTGGATATATATTGTTGCCATTGCAAGACCCTTCGGAAATTTGCTTACCGTCGTTGAAGGTGCTGGAACTACTACAAATGCATCACTTGAATTTGAGTTCCATGCATATCCTTCTCTCTGCCTGTCCTGTTCTCGAGAATCTGGAACTTTTCTTTAGTCCTGAATCTTTGGACATAATTCGGGTGCCATCTTCCTTGAAGAGGTTAAAAATCACTGTTGATAATAATGTTGGGGCTTGGCTTGAAATAGATGCAGCTGGTCTCAAGTACCTTAGCCTTGAAGGGATCACATTCCGTGATGCCGCGGCTGTTGGGAACTTGCACAGCGTGGAAGAAGCATATCTTGATGTATTTCCCACTCCTAAAAGTGAATCTGATGAGCCTTTACTCAATCTCCTTCGAGCTCTTTCCGGAATTAAACGTCTAGAACTACATAATTCAACCACAAAGGTGACTGAAGTTTTCATTATTTGTAGTATATGTTGTTTTGTAGTAATAATTATTAAATAATCATTTAATCACTTGCTTTTGCCGTGTACAAGCAGTGGCTATTTGCTGCCCCCATTTTACATTTTCCGGAGTTTCACTATTTGCTTCATCTAAAGCTTAAGTATCCCCCTTTCAACTCAACTTTTTTGTTCGACGTGCTTCAAAAATGTCCAATGCTAGAAACTCTCATAGCATTTAGTTTCGACATCGACATGGTTTGTCCAGTTTAACTCGAGCTTTGTTGTGTTCGTGTTTTTTTTGTTTGGATCTTATATATCAGTTGCCCCTTGTTGCCAGGTGGATCGATCATTTGATTCGTTGCCTTCATATAGATGGGAGGCAAAGTCTAAAAGTGTTCCTAAATGCCTCATATCCCATTTGACCTTTATTCACTTTTACGGATGTCCAGAAAATGAGCTGGAATTTATTGGGTATATTTTGCAACATGGACTTGTTTTGAAGACAATGATTGTTGATGAATATTGGCGAGACCAACCAGACCGCTGGATGAAAAAAATTTCAGATCTACCAAGAGGATCTGCCATGTGCCAAGTTAAATTTCTCTGA
Protein sequence

>MS.gene053313.t1

MSLVSRKWRNLWKNLEVFDFRDKCNEYSYQAPDDEFNEQFMLFTVFVNTVLSLRRSRVVRKFRLSSDHILNDPFFAYSVDTWLSIAIGPHLQEFNLTLFTAGAFNNLPLTLFSCSNLVLELLQMHHLNLSSMHILLSACPVLENLELFFSPESLDIIRVPSSLKRLKITVDNNVGAWLEIDAAGLKYLSLEGITFRDAAAVGNLHSVEEAYLDVFPTPKSESDEPLLNLLRALSGIKRLELHNSTTKWLFAAPILHFPEFHYLLHLKLKYPPFNSTFLFDVLQKCPMLETLIAFSFDIDMVDRSFDSLPSYRWEAKSKSVPKCLISHLTFIHFYGCPENELEFIGYILQHGLVLKTMIVDEYWRDQPDRWMKKISDLPRGSAMCQVKFL