AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89076

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89076.t1 MTR_3g082890 45.255 274 123 5 8 255 212 484 5.45e-63 205
MS.gene89076.t1 MTR_3g115430 72.000 125 35 0 131 255 1 125 6.54e-62 191
MS.gene89076.t1 MTR_3g082890 39.871 311 123 6 8 255 132 441 5.18e-56 186
MS.gene89076.t1 MTR_7g098510 42.424 264 129 11 8 255 135 391 1.14e-53 178
MS.gene89076.t1 MTR_7g081290 64.964 137 42 4 122 255 123 256 2.43e-52 171
MS.gene89076.t1 MTR_2g020180 52.353 170 72 2 86 255 55 215 1.51e-51 167
MS.gene89076.t1 MTR_7g034120 39.844 256 134 8 10 255 63 308 1.28e-45 155
MS.gene89076.t1 MTR_7g032380 39.200 250 119 8 12 255 134 356 5.99e-45 154
MS.gene89076.t1 MTR_7g098570 41.176 255 130 9 8 249 137 384 7.14e-45 157
MS.gene89076.t1 MTR_7g056433 39.544 263 131 8 12 255 136 389 1.17e-44 154
MS.gene89076.t1 MTR_1g041245 41.762 261 128 11 12 255 133 386 1.95e-44 154
MS.gene89076.t1 MTR_7g033915 41.176 255 114 10 10 254 136 364 4.21e-44 152
MS.gene89076.t1 MTR_7g032370 40.310 258 123 11 12 255 112 352 1.94e-43 150
MS.gene89076.t1 MTR_7g034115 39.922 258 127 9 12 253 133 378 4.86e-43 150
MS.gene89076.t1 MTR_4g134150 66.957 115 36 2 1 114 117 230 2.27e-42 144
MS.gene89076.t1 MTR_7g098480 42.460 252 129 9 8 249 135 380 3.01e-42 150
MS.gene89076.t1 MTR_7g033990 40.088 227 112 6 34 249 161 374 6.56e-41 145
MS.gene89076.t1 MTR_0543s0010 37.698 252 143 7 12 254 203 449 1.03e-38 140
MS.gene89076.t1 MTR_5g008950 33.333 255 153 8 12 255 117 365 1.12e-38 139
MS.gene89076.t1 MTR_7g115270 37.023 262 141 6 8 249 136 393 2.86e-37 135
MS.gene89076.t1 MTR_3g087260 39.080 261 144 7 6 254 199 456 3.00e-36 134
MS.gene89076.t1 MTR_5g008920 34.601 263 151 8 6 255 127 381 3.16e-36 132
MS.gene89076.t1 MTR_5g013500 38.306 248 120 8 35 255 124 365 1.06e-35 130
MS.gene89076.t1 MTR_1g043170 63.095 84 31 0 172 255 5 88 2.56e-35 121
MS.gene89076.t1 MTR_5g012840 36.187 257 119 9 4 255 102 318 5.13e-35 127
MS.gene89076.t1 MTR_3g036250 36.546 249 136 8 8 249 124 357 1.17e-34 127
MS.gene89076.t1 MTR_0492s0020 37.395 238 138 6 12 243 203 435 1.51e-34 129
MS.gene89076.t1 MTR_7g088260 35.249 261 150 9 8 255 208 462 3.22e-34 128
MS.gene89076.t1 MTR_3g108560 36.398 261 147 7 9 254 132 388 1.82e-32 122
MS.gene89076.t1 MTR_5g007410 35.472 265 136 9 8 248 145 398 5.22e-32 122
MS.gene89076.t1 MTR_7g114710 35.887 248 127 8 16 255 141 364 1.46e-31 119
MS.gene89076.t1 MTR_7g056420 35.769 260 122 9 8 249 124 356 1.48e-31 123
MS.gene89076.t1 MTR_7g056430 35.769 260 122 9 8 249 124 356 1.58e-31 122
MS.gene89076.t1 MTR_1g108820 35.075 268 133 10 8 248 123 376 2.73e-31 119
MS.gene89076.t1 MTR_3g467400 35.985 264 123 8 8 255 134 367 4.13e-31 118
MS.gene89076.t1 MTR_3g107120 35.249 261 137 10 12 255 146 391 1.44e-30 117
MS.gene89076.t1 MTR_1g066830 31.273 275 150 9 6 254 125 386 3.06e-30 116
MS.gene89076.t1 MTR_4g080610 37.447 235 132 10 34 254 123 356 9.61e-30 114
MS.gene89076.t1 MTR_3g023630 35.484 248 144 7 8 249 128 365 1.58e-29 114
MS.gene89076.t1 MTR_3g040700 35.909 220 124 7 8 221 162 370 4.03e-29 114
MS.gene89076.t1 MTR_3g109982 37.402 254 139 10 8 248 220 466 9.62e-29 114
MS.gene89076.t1 MTR_3g113785 58.889 90 29 1 134 215 3 92 1.34e-28 104
MS.gene89076.t1 MTR_7g056020 35.827 254 147 8 12 255 124 371 3.18e-28 113
MS.gene89076.t1 MTR_5g058100 30.827 266 149 7 12 255 132 384 3.30e-28 110
MS.gene89076.t1 MTR_7g007080 32.283 254 140 8 12 249 133 370 1.46e-27 109
MS.gene89076.t1 MTR_6g045333 32.963 270 151 9 8 254 183 445 1.13e-26 107
MS.gene89076.t1 MTR_1g108800 35.294 255 131 10 12 248 94 332 2.60e-26 105
MS.gene89076.t1 MTR_3g108150 33.585 265 154 8 7 254 127 386 3.28e-26 105
MS.gene89076.t1 MTR_3g105060 33.716 261 142 9 7 255 132 373 4.93e-26 105
MS.gene89076.t1 MTR_7g026500 32.319 263 152 8 8 254 142 394 1.11e-25 104
MS.gene89076.t1 MTR_4g039440 30.189 265 159 6 12 255 153 412 1.42e-25 104
MS.gene89076.t1 MTR_3g107100 35.000 240 133 7 1 225 125 356 1.89e-25 103
MS.gene89076.t1 MTR_2g007450 28.179 291 157 8 2 255 146 421 3.29e-25 103
MS.gene89076.t1 MTR_5g073420 32.895 228 126 7 8 225 134 344 8.03e-25 101
MS.gene89076.t1 MTR_5g045170 31.765 255 154 8 8 248 133 381 2.19e-24 100
MS.gene89076.t1 MTR_6g040270 28.996 269 154 8 6 252 125 378 3.38e-24 100
MS.gene89076.t1 MTR_6g022500 30.916 262 148 7 5 247 218 465 6.51e-24 100
MS.gene89076.t1 MTR_7g083740 31.535 241 125 8 37 254 143 366 2.31e-23 97.4
MS.gene89076.t1 MTR_1g053025 29.119 261 156 8 14 253 135 387 3.30e-23 97.4
MS.gene89076.t1 MTR_5g058000 31.955 266 145 7 8 254 126 374 3.54e-23 97.1
MS.gene89076.t1 MTR_3g107160 30.534 262 148 11 12 255 138 383 4.87e-23 97.1
MS.gene89076.t1 MTR_3g071850 29.811 265 153 7 7 248 145 399 6.27e-23 97.8
MS.gene89076.t1 MTR_8g085430 47.619 105 47 2 149 246 213 316 7.19e-23 95.5
MS.gene89076.t1 MTR_5g063950 29.545 264 164 8 7 253 102 360 8.23e-23 95.9
MS.gene89076.t1 MTR_7g056277 31.660 259 145 7 12 254 385 627 1.69e-21 93.6
MS.gene89076.t1 MTR_1g085120 30.000 270 163 6 5 255 209 471 2.02e-21 93.2
MS.gene89076.t1 MTR_1g085120 30.000 270 163 6 5 255 284 546 2.70e-21 92.8
MS.gene89076.t1 MTR_2g036470 29.545 264 163 6 1 248 130 386 4.42e-21 91.7
MS.gene89076.t1 MTR_7g034110 40.984 122 65 3 127 248 218 332 7.06e-21 90.5
MS.gene89076.t1 MTR_2g007400 30.147 272 143 12 10 252 147 400 1.00e-20 90.5
MS.gene89076.t1 MTR_1089s0010 30.739 257 157 9 12 255 131 379 1.07e-20 90.5
MS.gene89076.t1 MTR_3g113770 33.023 215 73 7 12 215 180 334 1.09e-20 89.7
MS.gene89076.t1 MTR_5g054920 30.108 186 105 6 55 225 2 177 1.14e-20 87.0
MS.gene89076.t1 MTR_0009s0080 32.245 245 124 8 8 225 138 367 1.14e-20 90.5
MS.gene89076.t1 MTR_5g039490 30.364 247 144 6 8 248 142 366 1.19e-20 90.1
MS.gene89076.t1 MTR_7g056287 30.888 259 147 8 12 254 129 371 2.22e-20 89.4
MS.gene89076.t1 MTR_5g073370 33.047 233 138 7 3 225 123 347 5.05e-20 88.6
MS.gene89076.t1 MTR_5g039450 29.032 248 130 9 32 253 129 356 5.60e-20 88.2
MS.gene89076.t1 MTR_6g011460 31.624 234 142 6 5 228 163 388 9.97e-20 87.8
MS.gene89076.t1 MTR_5g031970 30.705 241 149 7 8 247 122 345 2.68e-19 86.3
MS.gene89076.t1 MTR_1g107385 29.323 266 156 9 8 252 166 420 3.50e-19 86.3
MS.gene89076.t1 MTR_2g007550 31.102 254 116 11 12 255 131 335 2.16e-18 83.6
MS.gene89076.t1 MTR_5g083890 27.372 274 155 9 8 254 142 398 7.11e-18 82.8
MS.gene89076.t1 MTR_7g055943 29.167 264 161 8 12 255 164 421 7.38e-18 82.8
MS.gene89076.t1 MTR_7g007340 29.572 257 150 9 8 249 108 348 1.54e-17 81.3
MS.gene89076.t1 MTR_3g107940 42.857 105 50 2 146 249 109 204 1.65e-17 80.9
MS.gene89076.t1 MTR_3g106960 35.294 136 75 4 125 253 64 193 2.15e-17 79.3
MS.gene89076.t1 MTR_6g044572 27.799 259 163 8 5 255 146 388 2.92e-17 80.9
MS.gene89076.t1 MTR_6g022360 30.403 273 155 9 5 254 147 407 6.99e-17 79.7
MS.gene89076.t1 MTR_6g044560 28.571 259 161 8 5 255 322 564 9.77e-17 79.7
MS.gene89076.t1 MTR_2g008320 29.924 264 152 11 12 255 177 427 1.23e-16 79.0
MS.gene89076.t1 MTR_6g022520 28.996 269 168 6 4 254 49 312 2.12e-16 77.4
MS.gene89076.t1 MTR_5g008910 29.004 231 124 8 58 255 79 302 2.19e-16 77.4
MS.gene89076.t1 MTR_3g026920 28.390 236 129 6 12 227 168 383 2.94e-16 77.8
MS.gene89076.t1 MTR_5g039470 28.390 236 138 8 8 221 101 327 3.74e-16 77.0
MS.gene89076.t1 MTR_5g061680 34.409 186 99 5 8 193 132 294 5.18e-16 76.6
MS.gene89076.t1 MTR_7g075820 26.217 267 158 11 8 252 142 391 8.53e-16 76.6
MS.gene89076.t1 MTR_2g007360 26.792 265 148 10 17 249 115 365 9.90e-16 76.3
MS.gene89076.t1 MTR_1377s0010 35.577 104 61 2 122 225 30 127 1.54e-15 71.6
MS.gene89076.t1 MTR_3g108140 43.210 81 43 1 177 254 67 147 1.66e-15 72.8
MS.gene89076.t1 MTR_3g099830 28.938 273 139 12 12 252 122 371 2.02e-15 75.1
MS.gene89076.t1 MTR_5g076150 31.308 214 108 7 8 190 22 227 3.69e-15 72.8
MS.gene89076.t1 MTR_2g005990 29.924 264 148 9 8 249 138 386 3.88e-15 74.7
MS.gene89076.t1 MTR_4g096730 46.341 82 36 2 178 255 12 89 1.61e-14 67.8
MS.gene89076.t1 MTR_8g077460 27.381 252 154 10 12 255 145 375 1.96e-14 72.4
MS.gene89076.t1 MTR_2g007090 27.311 238 126 6 34 254 86 293 2.08e-14 71.6
MS.gene89076.t1 MTR_2g008310 26.996 263 159 9 12 255 123 371 2.73e-14 72.0
MS.gene89076.t1 MTR_5g073340 29.630 216 114 6 12 202 106 308 3.13e-14 71.2
MS.gene89076.t1 MTR_5g024060 31.538 130 82 2 119 248 76 198 6.09e-14 69.7
MS.gene89076.t1 MTR_1g108820 32.796 186 89 6 8 167 123 298 1.37e-13 69.7
MS.gene89076.t1 MTR_3g095630 34.375 128 76 4 122 248 55 175 1.74e-13 67.4
MS.gene89076.t1 MTR_8g014210 26.941 219 115 6 74 255 21 231 3.22e-13 67.4
MS.gene89076.t1 MTR_1g043180 48.214 56 29 0 117 172 42 97 7.75e-13 65.1
MS.gene89076.t1 MTR_2g102690 74.359 39 10 0 1 39 61 99 1.03e-12 63.9
MS.gene89076.t1 MTR_2g102690 70.270 37 11 0 182 218 96 132 7.17e-11 58.9
MS.gene89076.t1 MTR_3g094980 36.667 90 52 1 147 231 34 123 2.00e-12 64.3
MS.gene89076.t1 MTR_3g108560 34.118 170 97 5 9 167 132 297 5.00e-12 65.1
MS.gene89076.t1 MTR_7g056280 26.738 187 110 5 59 225 367 546 9.32e-12 65.1
MS.gene89076.t1 MTR_4g055580 25.172 290 146 12 11 230 255 543 1.42e-11 64.3
MS.gene89076.t1 MTR_7g022105 34.146 123 71 4 124 243 296 411 1.65e-11 63.9
MS.gene89076.t1 MTR_7g021990 29.457 258 153 9 11 248 167 415 8.46e-11 62.0
MS.gene89076.t1 MTR_7g021950 29.457 258 153 9 11 248 167 415 9.09e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89076.t1 AT4G26340 26.885 305 163 11 7 255 119 419 1.01e-16 79.3
MS.gene89076.t1 AT1G32375 23.510 302 161 11 15 255 130 422 8.86e-14 70.9
MS.gene89076.t1 AT1G32375 23.510 302 161 11 15 255 130 422 8.86e-14 70.9
MS.gene89076.t1 AT4G10400 28.053 303 159 12 3 255 116 409 8.78e-13 67.8
MS.gene89076.t1 AT4G10400 28.053 303 159 12 3 255 116 409 8.78e-13 67.8
MS.gene89076.t1 AT4G10400 28.053 303 159 12 3 255 116 409 8.78e-13 67.8
MS.gene89076.t1 AT4G10420 25.987 304 168 10 3 254 23 321 1.04e-11 64.3
MS.gene89076.t1 AT3G54910 27.922 308 157 10 3 255 76 373 2.20e-11 63.5
MS.gene89076.t1 AT3G54910 27.922 308 157 10 3 255 76 373 2.20e-11 63.5
MS.gene89076.t1 AT3G54910 27.922 308 157 10 3 255 76 373 2.20e-11 63.5

Find 52 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCTTTGCATTTAAATTTA+AGG 0.083917 2.4:+2345308 None:intergenic
TTGAATTAAACTGTCAAAAT+TGG 0.098936 2.4:+2345725 None:intergenic
TCATGAATATATTGACTTTC+AGG 0.143726 2.4:+2345896 None:intergenic
ATCGCCAAATTGGGCGTTTC+TGG 0.184147 2.4:+2345929 None:intergenic
CTAAGAACTTGCTTGTTTAC+AGG 0.210792 2.4:-2345423 MS.gene89076:CDS
AATACTTGTTATCGCCAAAT+TGG 0.225019 2.4:+2345919 None:intergenic
GACATGTTCGAGAAGTTTAA+TGG 0.229648 2.4:-2346178 MS.gene89076:CDS
ATGCATTCCGGAGCAATATC+TGG 0.246429 2.4:+2345456 None:intergenic
TGTTTACAGGACATCGAAAA+TGG 0.276176 2.4:-2345504 MS.gene89076:intron
AAGAGAATCTTGTCAATATC+TGG 0.290913 2.4:+2346016 None:intergenic
TACTCCAGAAACGCCCAATT+TGG 0.301204 2.4:-2345933 MS.gene89076:CDS
TGCATTTAAATTTAAGGAAT+AGG 0.319894 2.4:+2345314 None:intergenic
TGAATTAAACTGTCAAAATT+GGG 0.326843 2.4:+2345726 None:intergenic
GATATTGACAAGATTCTCTT+AGG 0.328674 2.4:-2346013 MS.gene89076:CDS
ATGGAATTTATGAGGACAAT+TGG 0.341820 2.4:-2345485 MS.gene89076:CDS
TATACTCAAAGTTCTCTGTT+TGG 0.360098 2.4:-2346062 MS.gene89076:CDS
TTAATTTAAAAGGTAAAAGT+TGG 0.360917 2.4:-2345686 MS.gene89076:CDS
AGAGAATATGCGTTTAAAAC+TGG 0.387449 2.4:-2345855 MS.gene89076:intron
TGTTTGCAATTCCTCTAGAA+TGG 0.393315 2.4:+2345985 None:intergenic
GTTTGCAATTCCTCTAGAAT+GGG 0.408530 2.4:+2345986 None:intergenic
AATTGTCGTCTTTCACAGAT+AGG 0.413879 2.4:+2346101 None:intergenic
AGCTGAGATGAAATGCATTC+CGG 0.438266 2.4:+2345444 None:intergenic
ATACTTGTTATCGCCAAATT+GGG 0.441599 2.4:+2345920 None:intergenic
TAATTTAAAAGGTAAAAGTT+GGG 0.447111 2.4:-2345685 MS.gene89076:CDS
AAACAGAGAACTTTGAGTAT+AGG 0.461425 2.4:+2346064 None:intergenic
CATCGAAAATGGAATTTATG+AGG 0.500230 2.4:-2345493 MS.gene89076:CDS
GGAAATTATCTGTGTGCCCA+AGG 0.500405 2.4:-2345275 MS.gene89076:CDS
TTGGATAATATGTTGTTCAA+TGG 0.500733 2.4:-2346043 MS.gene89076:CDS
ATGCAAAGCACGAAATGTTA+CGG 0.503024 2.4:-2345296 MS.gene89076:CDS
ATGGTAAGATGTTGAAGCTT+GGG 0.515978 2.4:+2345621 None:intergenic
CATATGTAATACCTCATGAA+TGG 0.521023 2.4:+2345602 None:intergenic
GTAAAAGTTGGGATAAGAAG+TGG 0.524392 2.4:-2345674 MS.gene89076:CDS
AATATAAGTTCACAGCCCCT+TGG 0.525982 2.4:+2345258 None:intergenic
GTTGGGATAAGAAGTGGAAG+TGG 0.534670 2.4:-2345668 MS.gene89076:CDS
TGCATTCCGGAGCAATATCT+GGG 0.540986 2.4:+2345457 None:intergenic
ATATAAGTTCACAGCCCCTT+GGG 0.543168 2.4:+2345259 None:intergenic
GAAATTATCTGTGTGCCCAA+GGG 0.545744 2.4:-2345274 MS.gene89076:CDS
TTCATGAATACTTCCTACCT+AGG 0.553778 2.4:-2345880 MS.gene89076:CDS
CTTAGGCTGCCCCATTCTAG+AGG 0.573194 2.4:-2345996 MS.gene89076:CDS
AATGGTAAGATGTTGAAGCT+TGG 0.575823 2.4:+2345620 None:intergenic
GAATTAAACTGTCAAAATTG+GGG 0.577717 2.4:+2345727 None:intergenic
GAAGGACCCAGATATTGCTC+CGG 0.586927 2.4:-2345463 MS.gene89076:CDS
AAACGCATATTCTCTACCCT+AGG 0.589888 2.4:+2345863 None:intergenic
TGCTTGTTTACAGGTTGCAG+AGG 0.594080 2.4:-2345414 MS.gene89076:CDS
AATTTATGAGGACAATTGGA+AGG 0.595790 2.4:-2345481 MS.gene89076:CDS
ATAGGCACTGTGAATTGTCA+TGG 0.604532 2.4:+2345332 None:intergenic
GCATATTCTCTACCCTAGGT+AGG 0.607522 2.4:+2345867 None:intergenic
TCATGAATACTTCCTACCTA+GGG 0.617474 2.4:-2345879 MS.gene89076:CDS
AAATTATCTGTGTGCCCAAG+GGG 0.625212 2.4:-2345273 MS.gene89076:CDS
TCAACATCTTACCATTCATG+AGG 0.633550 2.4:-2345613 MS.gene89076:intron
TGGGGACTAGTAAGTACATG+TGG 0.637068 2.4:+2345745 None:intergenic
TTTGCAATTCCTCTAGAATG+GGG 0.642459 2.4:+2345987 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTATTTTTCTTTAATTTAAA+AGG - chr2.4:2345730-2345749 MS.gene89076:CDS 10.0%
!! TAATTTAAAAGGTAAAAGTT+GGG - chr2.4:2345741-2345760 MS.gene89076:CDS 15.0%
!! TTAATTTAAAAGGTAAAAGT+TGG - chr2.4:2345740-2345759 MS.gene89076:CDS 15.0%
!! TGAATTAAACTGTCAAAATT+GGG + chr2.4:2345703-2345722 None:intergenic 20.0%
!! TGCATTTAAATTTAAGGAAT+AGG + chr2.4:2346115-2346134 None:intergenic 20.0%
!! TTGAATTAAACTGTCAAAAT+TGG + chr2.4:2345704-2345723 None:intergenic 20.0%
!!! TTTTGACAGTTTAATTCAAT+TGG - chr2.4:2345705-2345724 MS.gene89076:CDS 20.0%
! GAATTAAACTGTCAAAATTG+GGG + chr2.4:2345702-2345721 None:intergenic 25.0%
! GTGCTTTGCATTTAAATTTA+AGG + chr2.4:2346121-2346140 None:intergenic 25.0%
! TAATTCACTTGATTGTTTAC+AGG - chr2.4:2345909-2345928 MS.gene89076:CDS 25.0%
! TTGGATAATATGTTGTTCAA+TGG - chr2.4:2345383-2345402 MS.gene89076:CDS 25.0%
!! ATGAGGTATTACATATGTTT+AGG - chr2.4:2345830-2345849 MS.gene89076:intron 25.0%
!!! TCATGAATATATTGACTTTC+AGG + chr2.4:2345533-2345552 None:intergenic 25.0%
AAACAGAGAACTTTGAGTAT+AGG + chr2.4:2345365-2345384 None:intergenic 30.0%
AAGAGAATCTTGTCAATATC+TGG + chr2.4:2345413-2345432 None:intergenic 30.0%
AATTTATGAGGACAATTGGA+AGG - chr2.4:2345945-2345964 MS.gene89076:CDS 30.0%
ATGGAATTTATGAGGACAAT+TGG - chr2.4:2345941-2345960 MS.gene89076:CDS 30.0%
CAAAGATACATCGATAATAG+AGG + chr2.4:2345886-2345905 None:intergenic 30.0%
CATATGTAATACCTCATGAA+TGG + chr2.4:2345827-2345846 None:intergenic 30.0%
CATCGAAAATGGAATTTATG+AGG - chr2.4:2345933-2345952 MS.gene89076:CDS 30.0%
GATATTGACAAGATTCTCTT+AGG - chr2.4:2345413-2345432 MS.gene89076:CDS 30.0%
GTTTCGTCTATACAGAAATT+GGG - chr2.4:2345618-2345637 MS.gene89076:CDS 30.0%
TATACTCAAAGTTCTCTGTT+TGG - chr2.4:2345364-2345383 MS.gene89076:CDS 30.0%
TGTTTCGTCTATACAGAAAT+TGG - chr2.4:2345617-2345636 MS.gene89076:CDS 30.0%
! AGAGAATATGCGTTTAAAAC+TGG - chr2.4:2345571-2345590 MS.gene89076:intron 30.0%
!! AATACTTGTTATCGCCAAAT+TGG + chr2.4:2345510-2345529 None:intergenic 30.0%
!! ATACTTGTTATCGCCAAATT+GGG + chr2.4:2345509-2345528 None:intergenic 30.0%
!! TAGGTTTTTCAACTTGAGAA+CGG + chr2.4:2345309-2345328 None:intergenic 30.0%
AATGGTAAGATGTTGAAGCT+TGG + chr2.4:2345809-2345828 None:intergenic 35.0%
AATTGTCGTCTTTCACAGAT+AGG + chr2.4:2345328-2345347 None:intergenic 35.0%
ATGCAAAGCACGAAATGTTA+CGG - chr2.4:2346130-2346149 MS.gene89076:CDS 35.0%
ATGGTAAGATGTTGAAGCTT+GGG + chr2.4:2345808-2345827 None:intergenic 35.0%
GTAAAAGTTGGGATAAGAAG+TGG - chr2.4:2345752-2345771 MS.gene89076:CDS 35.0%
GTCTATACAGAAATTGGGTA+TGG - chr2.4:2345623-2345642 MS.gene89076:CDS 35.0%
TCAACATCTTACCATTCATG+AGG - chr2.4:2345813-2345832 MS.gene89076:intron 35.0%
TCATGAATACTTCCTACCTA+GGG - chr2.4:2345547-2345566 MS.gene89076:intron 35.0%
TGTTTACAGGACATCGAAAA+TGG - chr2.4:2345922-2345941 MS.gene89076:CDS 35.0%
TTCATGAATACTTCCTACCT+AGG - chr2.4:2345546-2345565 MS.gene89076:intron 35.0%
! CTAAGAACTTGCTTGTTTAC+AGG - chr2.4:2346003-2346022 MS.gene89076:CDS 35.0%
! TGTTTGCAATTCCTCTAGAA+TGG + chr2.4:2345444-2345463 None:intergenic 35.0%
!! GTTTGCAATTCCTCTAGAAT+GGG + chr2.4:2345443-2345462 None:intergenic 35.0%
!! TTTGCAATTCCTCTAGAATG+GGG + chr2.4:2345442-2345461 None:intergenic 35.0%
AAACGCATATTCTCTACCCT+AGG + chr2.4:2345566-2345585 None:intergenic 40.0%
AAATTATCTGTGTGCCCAAG+GGG - chr2.4:2346153-2346172 MS.gene89076:CDS 40.0%
AATATAAGTTCACAGCCCCT+TGG + chr2.4:2346171-2346190 None:intergenic 40.0%
AGCTGAGATGAAATGCATTC+CGG + chr2.4:2345985-2346004 None:intergenic 40.0%
ATATAAGTTCACAGCCCCTT+GGG + chr2.4:2346170-2346189 None:intergenic 40.0%
CTTGCAACTAAAAATGCGAC+TGG + chr2.4:2345279-2345298 None:intergenic 40.0%
GAAATTATCTGTGTGCCCAA+GGG - chr2.4:2346152-2346171 MS.gene89076:CDS 40.0%
! ATAGGCACTGTGAATTGTCA+TGG + chr2.4:2346097-2346116 None:intergenic 40.0%
ATGCATTCCGGAGCAATATC+TGG + chr2.4:2345973-2345992 None:intergenic 45.0%
GCATATTCTCTACCCTAGGT+AGG + chr2.4:2345562-2345581 None:intergenic 45.0%
GGAAATTATCTGTGTGCCCA+AGG - chr2.4:2346151-2346170 MS.gene89076:CDS 45.0%
GTTGGGATAAGAAGTGGAAG+TGG - chr2.4:2345758-2345777 MS.gene89076:intron 45.0%
TACTCCAGAAACGCCCAATT+TGG - chr2.4:2345493-2345512 MS.gene89076:CDS 45.0%
TGCATTCCGGAGCAATATCT+GGG + chr2.4:2345972-2345991 None:intergenic 45.0%
TGCTTGTTTACAGGTTGCAG+AGG - chr2.4:2346012-2346031 MS.gene89076:CDS 45.0%
! TGGGGACTAGTAAGTACATG+TGG + chr2.4:2345684-2345703 None:intergenic 45.0%
ATCGCCAAATTGGGCGTTTC+TGG + chr2.4:2345500-2345519 None:intergenic 50.0%
GAAGGACCCAGATATTGCTC+CGG - chr2.4:2345963-2345982 MS.gene89076:CDS 50.0%
CTTAGGCTGCCCCATTCTAG+AGG - chr2.4:2345430-2345449 MS.gene89076:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 2345251 2346197 2345251 ID=MS.gene89076
chr2.4 mRNA 2345251 2346197 2345251 ID=MS.gene89076.t1;Parent=MS.gene89076
chr2.4 exon 2345856 2346197 2345856 ID=MS.gene89076.t1.exon1;Parent=MS.gene89076.t1
chr2.4 CDS 2345856 2346197 2345856 ID=cds.MS.gene89076.t1;Parent=MS.gene89076.t1
chr2.4 exon 2345614 2345772 2345614 ID=MS.gene89076.t1.exon2;Parent=MS.gene89076.t1
chr2.4 CDS 2345614 2345772 2345614 ID=cds.MS.gene89076.t1;Parent=MS.gene89076.t1
chr2.4 exon 2345251 2345517 2345251 ID=MS.gene89076.t1.exon3;Parent=MS.gene89076.t1
chr2.4 CDS 2345251 2345517 2345251 ID=cds.MS.gene89076.t1;Parent=MS.gene89076.t1
Gene Sequence

>MS.gene89076

ATGTTCGAGAAGTTTAATGGATTAACCAGTCGCATTTTTAGTTGCAAGACACTCACCGTTCTCAAGTTGAAAAACCTATCTGTGAAAGACGACAATTGTAATGTAGATCTTCCTATACTCAAAGTTCTCTGTTTGGATAATATGTTGTTCAATGGTAAACCAGATATTGACAAGATTCTCTTAGGCTGCCCCATTCTAGAGGAATTGCAAACAAGTCTTGCTGCATTATATTTAAAGAAGCTTACTCCAGAAACGCCCAATTTGGCGATAACAAGTATTCCTGAAAGTCAATATATTCATGAATACTTCCTACCTAGGGTAGAGAATATGCGTTTAAAACTGGTATGAAATACGTTTTATATGTAATGTTTCGTCTATACAGAAATTGGGTATGGTTTCTAATGTTTTATCTGTGATTCATGCAGAAACTCCACATGTACTTACTAGTCCCCAATTTTGACAGTTTAATTCAATTGGAGCTATTTTTCTTTAATTTAAAAGGTAAAAGTTGGGATAAGAAGTGGAAGTGGATGCTAGCAGTGCTTCAACATAGTCCCAAGCTTCAACATCTTACCATTCATGAGGTATTACATATGTTTAGGATAATTTGATGATTTATTTTGTTAAGTCAACCTCTATTATCGATGTATCTTTGTTTTAATTCACTTGATTGTTTACAGGACATCGAAAATGGAATTTATGAGGACAATTGGAAGGACCCAGATATTGCTCCGGAATGCATTTCATCTCAGCTAAGAACTTGCTTGTTTACAGGTTGCAGAGGCAGAAAGTCTGAGCTTCAATTTGCAGAGTATCTTATGAAAAATTCAAAAGTGTTACGTACCATGACAATTCACAGTGCCTATTCCTTAAATTTAAATGCAAAGCACGAAATGTTACGGAAATTATCTGTGTGCCCAAGGGGCTGTGAACTTATATTTGATTGA
Protein sequence

>MS.gene89076.t1

MFEKFNGLTSRIFSCKTLTVLKLKNLSVKDDNCNVDLPILKVLCLDNMLFNGKPDIDKILLGCPILEELQTSLAALYLKKLTPETPNLAITSIPESQYIHEYFLPRVENMRLKLKLHMYLLVPNFDSLIQLELFFFNLKGKSWDKKWKWMLAVLQHSPKLQHLTIHEDIENGIYEDNWKDPDIAPECISSQLRTCLFTGCRGRKSELQFAEYLMKNSKVLRTMTIHSAYSLNLNAKHEMLRKLSVCPRGCELIFD