AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86337

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86337.t1 MTR_3g082890 45.697 337 144 8 67 369 153 484 1.88e-77 246
MS.gene86337.t1 MTR_4g134150 71.264 174 48 2 56 228 58 230 8.03e-77 236
MS.gene86337.t1 MTR_3g082890 41.176 374 144 9 67 369 73 441 1.59e-70 227
MS.gene86337.t1 MTR_1g041245 43.114 334 162 13 57 369 60 386 7.12e-66 214
MS.gene86337.t1 MTR_7g098510 40.663 332 171 12 57 369 67 391 1.28e-65 213
MS.gene86337.t1 MTR_7g033915 40.060 332 154 12 56 368 59 364 4.00e-62 203
MS.gene86337.t1 MTR_3g115430 69.600 125 38 0 245 369 1 125 1.13e-59 189
MS.gene86337.t1 MTR_7g032380 40.432 324 155 12 57 369 60 356 1.59e-59 196
MS.gene86337.t1 MTR_7g034115 40.000 335 161 12 57 367 60 378 6.30e-59 195
MS.gene86337.t1 MTR_7g032370 40.663 332 160 14 57 369 39 352 4.73e-58 192
MS.gene86337.t1 MTR_7g033990 39.514 329 163 9 57 363 60 374 1.36e-57 193
MS.gene86337.t1 MTR_7g098570 40.312 320 162 13 63 363 75 384 5.23e-57 193
MS.gene86337.t1 MTR_7g115270 37.758 339 175 9 56 363 60 393 3.05e-55 186
MS.gene86337.t1 MTR_7g034120 38.509 322 161 11 71 369 1 308 9.51e-55 182
MS.gene86337.t1 MTR_7g056433 37.353 340 177 12 56 369 60 389 1.97e-52 179
MS.gene86337.t1 MTR_7g081290 64.964 137 42 4 236 369 123 256 2.32e-52 174
MS.gene86337.t1 MTR_7g098480 41.121 321 168 12 57 363 67 380 3.39e-52 180
MS.gene86337.t1 MTR_0543s0010 37.267 322 185 9 57 368 135 449 1.10e-51 178
MS.gene86337.t1 MTR_2g020180 52.941 170 71 2 200 369 55 215 1.61e-51 171
MS.gene86337.t1 MTR_5g012840 35.180 361 167 16 22 369 12 318 5.24e-49 168
MS.gene86337.t1 MTR_7g088260 36.170 329 188 11 57 369 140 462 2.78e-48 169
MS.gene86337.t1 MTR_0492s0020 37.013 308 180 8 57 357 135 435 2.25e-47 166
MS.gene86337.t1 MTR_3g036250 34.985 343 186 12 33 363 40 357 4.90e-46 161
MS.gene86337.t1 MTR_7g056420 36.061 330 159 12 56 363 57 356 2.28e-45 167
MS.gene86337.t1 MTR_7g056430 36.061 330 159 12 56 363 57 356 3.06e-45 166
MS.gene86337.t1 MTR_3g108560 35.780 327 188 9 57 368 69 388 1.94e-44 157
MS.gene86337.t1 MTR_5g013500 34.593 344 174 11 56 369 43 365 2.00e-43 154
MS.gene86337.t1 MTR_3g023630 34.435 363 195 14 24 363 23 365 1.54e-42 152
MS.gene86337.t1 MTR_5g008920 33.233 331 193 11 57 369 61 381 2.06e-41 149
MS.gene86337.t1 MTR_8g085430 35.016 317 133 11 57 360 60 316 4.33e-41 147
MS.gene86337.t1 MTR_3g108150 34.925 335 185 11 57 368 62 386 3.51e-40 146
MS.gene86337.t1 MTR_3g040700 34.483 290 168 9 57 335 92 370 1.37e-38 143
MS.gene86337.t1 MTR_7g114710 34.848 330 173 13 56 369 61 364 4.27e-38 140
MS.gene86337.t1 MTR_5g008950 30.091 329 203 12 59 369 46 365 7.00e-38 140
MS.gene86337.t1 MTR_3g087260 38.768 276 153 8 106 368 184 456 2.52e-37 140
MS.gene86337.t1 MTR_3g087260 37.594 133 64 5 56 185 60 176 4.00e-11 64.7
MS.gene86337.t1 MTR_5g007410 33.631 336 176 13 58 362 79 398 1.83e-35 135
MS.gene86337.t1 MTR_1g043170 63.636 88 31 1 283 369 1 88 5.17e-35 124
MS.gene86337.t1 MTR_3g467400 30.000 360 165 11 55 369 50 367 2.13e-34 130
MS.gene86337.t1 MTR_3g107120 32.229 332 183 13 62 369 78 391 4.73e-34 130
MS.gene86337.t1 MTR_7g034110 33.123 317 157 11 57 362 60 332 1.34e-33 128
MS.gene86337.t1 MTR_1g066830 30.145 345 196 13 54 368 57 386 6.98e-33 127
MS.gene86337.t1 MTR_3g105060 32.326 331 188 12 56 369 62 373 7.76e-33 126
MS.gene86337.t1 MTR_3g107100 33.555 301 163 11 63 339 69 356 8.69e-32 124
MS.gene86337.t1 MTR_4g080610 32.515 326 176 13 57 368 61 356 9.24e-32 123
MS.gene86337.t1 MTR_6g045333 30.814 344 203 12 52 368 110 445 1.17e-31 124
MS.gene86337.t1 MTR_1g108820 32.302 291 156 8 99 362 100 376 1.64e-30 120
MS.gene86337.t1 MTR_2g007450 27.540 374 196 13 54 369 65 421 2.91e-30 120
MS.gene86337.t1 MTR_5g058100 30.449 312 185 9 79 369 84 384 5.43e-30 119
MS.gene86337.t1 MTR_7g056020 33.742 326 187 13 60 369 59 371 2.00e-29 119
MS.gene86337.t1 MTR_7g007080 29.664 327 186 11 63 363 62 370 3.14e-29 117
MS.gene86337.t1 MTR_6g022500 30.330 333 189 10 56 361 149 465 1.98e-28 115
MS.gene86337.t1 MTR_3g113785 58.889 90 29 1 248 329 3 92 3.61e-28 106
MS.gene86337.t1 MTR_7g026500 28.763 372 225 13 24 368 36 394 7.27e-28 113
MS.gene86337.t1 MTR_3g071850 28.107 338 205 10 52 362 73 399 1.96e-27 114
MS.gene86337.t1 MTR_3g107160 29.254 335 191 15 63 369 67 383 2.10e-27 111
MS.gene86337.t1 MTR_5g058000 31.190 311 174 9 79 368 83 374 6.56e-27 110
MS.gene86337.t1 MTR_5g073420 29.866 298 173 11 59 339 66 344 7.58e-27 110
MS.gene86337.t1 MTR_1g108800 33.803 284 152 11 97 362 67 332 1.67e-26 108
MS.gene86337.t1 MTR_3g109982 36.614 254 141 10 122 362 220 466 2.21e-26 111
MS.gene86337.t1 MTR_3g109982 40.602 133 76 3 56 186 61 192 1.68e-16 81.6
MS.gene86337.t1 MTR_5g045170 30.124 322 196 12 63 362 67 381 3.21e-26 108
MS.gene86337.t1 MTR_2g036470 29.664 327 201 9 58 362 67 386 9.01e-26 107
MS.gene86337.t1 MTR_4g039440 27.976 336 211 9 59 369 83 412 2.05e-25 106
MS.gene86337.t1 MTR_5g063950 31.034 290 173 11 97 368 81 361 3.10e-25 105
MS.gene86337.t1 MTR_6g040270 28.526 312 181 10 80 366 84 378 1.04e-24 104
MS.gene86337.t1 MTR_1g053025 27.811 338 200 13 63 367 61 387 2.61e-24 103
MS.gene86337.t1 MTR_1g085120 28.152 341 209 9 56 369 140 471 5.74e-24 103
MS.gene86337.t1 MTR_5g031970 30.312 320 200 10 48 361 43 345 5.84e-24 101
MS.gene86337.t1 MTR_1089s0010 29.070 344 205 14 53 369 48 379 7.35e-24 101
MS.gene86337.t1 MTR_1g085120 28.152 341 209 9 56 369 215 546 8.97e-24 102
MS.gene86337.t1 MTR_7g083740 28.523 298 167 9 94 368 92 366 1.52e-23 100
MS.gene86337.t1 MTR_6g011460 30.719 306 182 11 56 342 94 388 1.86e-23 101
MS.gene86337.t1 MTR_5g039450 28.487 337 175 13 58 367 59 356 2.80e-23 99.8
MS.gene86337.t1 MTR_0009s0080 32.090 268 138 9 99 339 117 367 7.08e-23 99.4
MS.gene86337.t1 MTR_3g108560 33.475 236 139 7 57 281 69 297 1.32e-22 97.1
MS.gene86337.t1 MTR_5g039490 28.931 318 189 9 58 362 73 366 1.51e-22 98.2
MS.gene86337.t1 MTR_7g056277 29.819 332 195 10 58 368 313 627 1.90e-22 99.4
MS.gene86337.t1 MTR_6g044572 28.819 288 179 9 90 369 119 388 2.54e-22 97.4
MS.gene86337.t1 MTR_5g039470 27.907 301 183 10 58 335 38 327 3.53e-22 96.3
MS.gene86337.t1 MTR_5g073370 30.508 295 180 10 57 339 66 347 4.36e-22 96.7
MS.gene86337.t1 MTR_2g007400 27.247 356 192 16 58 366 65 400 5.53e-22 96.7
MS.gene86337.t1 MTR_7g056287 29.552 335 192 12 58 368 57 371 9.95e-22 95.5
MS.gene86337.t1 MTR_6g044560 29.514 288 177 9 90 369 295 564 1.34e-21 96.7
MS.gene86337.t1 MTR_7g055943 28.012 332 206 11 63 369 98 421 9.13e-21 93.6
MS.gene86337.t1 MTR_3g113770 33.023 215 73 7 126 329 180 334 1.67e-20 91.7
MS.gene86337.t1 MTR_6g022520 28.840 319 199 9 71 368 1 312 2.23e-20 90.9
MS.gene86337.t1 MTR_2g007550 30.183 328 144 16 63 369 72 335 2.86e-20 91.3
MS.gene86337.t1 MTR_5g045510 28.395 324 183 11 58 360 65 360 3.00e-20 91.3
MS.gene86337.t1 MTR_5g054920 29.570 186 106 6 169 339 2 177 5.46e-20 87.0
MS.gene86337.t1 MTR_2g102690 70.690 58 15 1 96 153 44 99 3.25e-19 83.2
MS.gene86337.t1 MTR_6g022360 28.955 335 196 12 62 368 87 407 4.34e-19 88.2
MS.gene86337.t1 MTR_7g056447 41.085 129 73 2 56 181 57 185 9.87e-19 85.9
MS.gene86337.t1 MTR_1g107385 27.684 354 187 15 61 366 88 420 1.66e-18 86.7
MS.gene86337.t1 MTR_3g026920 26.284 331 168 10 63 341 77 383 1.80e-18 86.7
MS.gene86337.t1 MTR_5g065100 27.778 306 182 10 69 368 73 345 1.83e-18 86.7
MS.gene86337.t1 MTR_5g061680 30.315 254 141 9 62 307 69 294 9.75e-18 83.6
MS.gene86337.t1 MTR_7g034090 27.445 317 147 14 63 368 67 311 1.83e-17 83.6
MS.gene86337.t1 MTR_3g107940 41.905 105 51 2 260 363 109 204 1.02e-16 80.9
MS.gene86337.t1 MTR_7g007340 29.572 257 150 9 122 363 108 348 1.04e-16 80.9
MS.gene86337.t1 MTR_3g106960 35.294 136 75 4 239 367 64 193 1.52e-16 78.6
MS.gene86337.t1 MTR_3g110010 40.602 133 76 3 56 186 61 192 2.05e-16 81.3
MS.gene86337.t1 MTR_7g033945 40.870 115 57 4 56 160 59 172 2.76e-16 76.6
MS.gene86337.t1 MTR_5g083890 26.642 274 157 9 122 368 142 398 4.93e-16 79.7
MS.gene86337.t1 MTR_2g005990 28.144 334 186 13 65 366 81 392 5.46e-16 79.0
MS.gene86337.t1 MTR_5g008910 29.004 231 124 8 172 369 79 302 1.07e-15 77.4
MS.gene86337.t1 MTR_5g076150 31.308 214 108 7 122 304 22 227 3.33e-15 74.7
MS.gene86337.t1 MTR_8g077460 26.389 288 178 11 95 369 109 375 3.75e-15 76.3
MS.gene86337.t1 MTR_1377s0010 35.577 104 61 2 236 339 30 127 5.65e-15 71.6
MS.gene86337.t1 MTR_5g024060 33.077 130 80 2 233 362 76 198 6.88e-15 74.3
MS.gene86337.t1 MTR_2g007360 25.714 280 141 10 131 366 115 371 9.17e-15 75.1
MS.gene86337.t1 MTR_3g108140 41.975 81 44 1 291 368 67 147 1.86e-14 71.2
MS.gene86337.t1 MTR_5g061410 29.362 235 132 8 58 281 56 267 2.21e-14 73.2
MS.gene86337.t1 MTR_4g096730 46.341 82 36 2 292 369 12 89 6.43e-14 67.4
MS.gene86337.t1 MTR_5g073340 29.218 243 134 6 99 316 79 308 6.71e-14 72.0
MS.gene86337.t1 MTR_2g007090 26.891 238 127 6 148 368 86 293 1.14e-13 71.2
MS.gene86337.t1 MTR_2g008310 26.616 263 160 10 126 369 123 371 1.72e-13 71.6
MS.gene86337.t1 MTR_7g075820 24.906 265 164 10 122 366 142 391 3.76e-13 70.5
MS.gene86337.t1 MTR_1g108820 29.665 209 111 5 99 281 100 298 5.78e-13 69.7
MS.gene86337.t1 MTR_8g014210 26.941 219 115 6 188 369 21 231 1.77e-12 66.6
MS.gene86337.t1 MTR_3g094980 36.667 90 52 1 261 345 34 123 5.12e-12 64.3
MS.gene86337.t1 MTR_1g043180 46.429 56 30 0 231 286 42 97 8.55e-12 63.5
MS.gene86337.t1 MTR_7g056280 26.738 187 110 5 173 339 367 546 1.08e-11 66.6
MS.gene86337.t1 MTR_5g073400 39.450 109 64 2 80 187 7 114 1.44e-11 62.0
MS.gene86337.t1 MTR_2g007500 26.721 247 157 7 95 319 91 335 1.79e-11 65.1
MS.gene86337.t1 MTR_7g022105 34.146 123 71 4 238 357 296 411 4.32e-11 64.3
MS.gene86337.t1 MTR_7g022060 27.778 252 136 9 122 369 120 329 6.65e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86337.t1 AT4G26340 26.415 371 208 13 59 369 54 419 1.88e-21 95.5
MS.gene86337.t1 AT5G56370 27.557 352 187 8 80 368 74 420 6.86e-21 94.0
MS.gene86337.t1 AT5G56370 27.557 352 187 8 80 368 74 420 6.86e-21 94.0
MS.gene86337.t1 AT5G62970 26.647 334 182 11 80 353 100 430 4.33e-18 85.9
MS.gene86337.t1 AT4G10400 27.616 344 186 14 80 369 75 409 2.39e-14 74.3
MS.gene86337.t1 AT4G10400 27.616 344 186 14 80 369 75 409 2.39e-14 74.3
MS.gene86337.t1 AT4G10400 27.616 344 186 14 80 369 75 409 2.39e-14 74.3
MS.gene86337.t1 AT1G32375 22.977 383 212 15 60 369 50 422 2.45e-14 74.3
MS.gene86337.t1 AT1G32375 22.977 383 212 15 60 369 50 422 2.45e-14 74.3
MS.gene86337.t1 AT5G56560 22.764 369 226 8 55 369 55 418 4.96e-14 73.6
MS.gene86337.t1 AT5G38590 25.545 321 171 8 95 357 91 401 1.38e-13 72.0
MS.gene86337.t1 AT5G38590 35.252 139 84 4 95 227 91 229 1.50e-12 67.8
MS.gene86337.t1 AT3G26920 24.516 310 198 11 85 368 1 300 1.66e-12 67.8
MS.gene86337.t1 AT5G52460 23.636 275 179 8 88 343 65 327 1.95e-12 68.2
MS.gene86337.t1 AT5G38590 35.252 139 84 4 95 227 56 194 3.45e-12 66.2
MS.gene86337.t1 AT3G49030 24.519 416 228 17 23 368 41 440 5.61e-12 67.0
MS.gene86337.t1 AT3G54910 26.857 350 185 13 80 369 35 373 3.62e-11 64.3
MS.gene86337.t1 AT3G54910 26.857 350 185 13 80 369 35 373 3.62e-11 64.3
MS.gene86337.t1 AT3G54910 26.857 350 185 13 80 369 35 373 3.62e-11 64.3
MS.gene86337.t1 AT5G52460 23.308 266 173 8 95 341 4 257 5.57e-11 63.2
MS.gene86337.t1 AT5G56370 37.736 106 61 2 80 181 74 178 8.49e-11 63.2

Find 75 sgRNAs with CRISPR-Local

Find 296 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTACTTTGCATTTAAATTTA+AGG 0.094701 2.3:+1928621 None:intergenic
TTGAATTAAACTGTCAAAAT+TGG 0.098936 2.3:+1929038 None:intergenic
TCATGAATATATTGACTTTC+AGG 0.143726 2.3:+1929209 None:intergenic
CGATCATTTCGCCTCAAATT+TGG 0.183656 2.3:-1929590 MS.gene86337:CDS
ATCGCCAAATTGGGCGTTTC+TGG 0.184147 2.3:+1929242 None:intergenic
ATGCATTCCGGAACAATATC+TGG 0.207548 2.3:+1928769 None:intergenic
CTACGAACTTGCTTGTTTAC+AGG 0.209209 2.3:-1928736 MS.gene86337:CDS
AATACTTGTTATCGCCAAAT+TGG 0.225019 2.3:+1929232 None:intergenic
GACATGTTCGAGAAGTTTAA+TGG 0.229648 2.3:-1929491 MS.gene86337:CDS
GTGAGCTTGCAGCAGAATTT+TGG 0.258471 2.3:-1932386 MS.gene86337:CDS
TGTTTACAGGACATCGAAAA+TGG 0.276176 2.3:-1928817 MS.gene86337:intron
AAGAGAATCTTGTCAATATC+TGG 0.290913 2.3:+1929329 None:intergenic
TACTCCAGAAACGCCCAATT+TGG 0.301204 2.3:-1929246 MS.gene86337:CDS
TGAATTAAACTGTCAAAATT+GGG 0.326843 2.3:+1929039 None:intergenic
CTACGAGCATTTGGACTGTT+TGG 0.326868 2.3:-1932415 MS.gene86337:CDS
GATATTGACAAGATTCTCTT+AGG 0.328674 2.3:-1929326 MS.gene86337:CDS
TGCATTTAAATTTAAGGAAT+AGG 0.338502 2.3:+1928627 None:intergenic
ATGGAATTTATGAGGACAAT+TGG 0.341820 2.3:-1928798 MS.gene86337:CDS
GTTTCATTCCTACGAGCATT+TGG 0.352450 2.3:-1932424 MS.gene86337:CDS
TGATACAATGACCGAGTATA+TGG 0.354546 2.3:+1929634 None:intergenic
TATACTCAAAGTTCTCTGTT+TGG 0.360098 2.3:-1929375 MS.gene86337:CDS
TTAATTTAAAAGGTAAAAGT+TGG 0.360917 2.3:-1928999 MS.gene86337:CDS
AGAGAATATGCGTTTAAAAC+TGG 0.387449 2.3:-1929168 MS.gene86337:intron
TGTTTGCAATTCCTCTAGAA+TGG 0.393315 2.3:+1929298 None:intergenic
GTTTGCAATTCCTCTAGAAT+GGG 0.408530 2.3:+1929299 None:intergenic
TTTGAGAAGAATGAACTTGA+AGG 0.411222 2.3:-1932446 MS.gene86337:CDS
AATTGTCGTCTTTCACAGAT+AGG 0.413879 2.3:+1929414 None:intergenic
GTATAGCTGCATTAATAAAA+CGG 0.430678 2.3:+1929540 None:intergenic
AGCTGAGATGAAATGCATTC+CGG 0.440438 2.3:+1928757 None:intergenic
ATACTTGTTATCGCCAAATT+GGG 0.441599 2.3:+1929233 None:intergenic
CCAGGTGTCGTCGCCGTCCT+CGG 0.443646 2.3:-1931487 MS.gene86337:intron
TAATTTAAAAGGTAAAAGTT+GGG 0.447111 2.3:-1928998 MS.gene86337:CDS
AAACAGAGAACTTTGAGTAT+AGG 0.461425 2.3:+1929377 None:intergenic
GAAGGACCCAGATATTGTTC+CGG 0.464376 2.3:-1928776 MS.gene86337:CDS
CATATTCTATGCCAAATTTG+AGG 0.478293 2.3:+1929579 None:intergenic
TTGAGGCGAAATGATCGTAT+TGG 0.479233 2.3:+1929596 None:intergenic
GTCGTCGCCGTCCTCGGTGG+TGG 0.484110 2.3:-1931481 MS.gene86337:intron
AAAGGTGATGTAGTCTTTGA+AGG 0.494205 2.3:+1929664 None:intergenic
ATGGTAAAATGTTGAAGCTT+GGG 0.496449 2.3:+1928934 None:intergenic
CATCGAAAATGGAATTTATG+AGG 0.500230 2.3:-1928806 MS.gene86337:CDS
GGAAATTATCTGTGTGCCCA+AGG 0.500405 2.3:-1928588 MS.gene86337:CDS
TTGGATAATATGTTGTTCAA+TGG 0.500733 2.3:-1929356 MS.gene86337:CDS
ATGCAAAGTACGAAATGTTA+CGG 0.503024 2.3:-1928609 MS.gene86337:CDS
AATGGTAAAATGTTGAAGCT+TGG 0.512234 2.3:+1928933 None:intergenic
ATTAATGCAGCTATACAAAG+AGG 0.517042 2.3:-1929533 MS.gene86337:CDS
GAACGTACCACCACCGAGGA+CGG 0.520595 2.3:+1931474 None:intergenic
TGGTGGTAGGAAGAGAAGAA+AGG 0.520978 2.3:-1932493 MS.gene86337:CDS
CATATGTAATACCTCATGAA+TGG 0.521023 2.3:+1928915 None:intergenic
GTAAAAGTTGGGATAAGAAG+TGG 0.524392 2.3:-1928987 MS.gene86337:CDS
AATATAAGTTCACAGCCCCT+TGG 0.525982 2.3:+1928571 None:intergenic
AGCTGCATTAATAAAACGGT+TGG 0.531777 2.3:+1929544 None:intergenic
GTTGGGATAAGAAGTGGAAG+TGG 0.534670 2.3:-1928981 MS.gene86337:CDS
ATATAAGTTCACAGCCCCTT+GGG 0.543168 2.3:+1928572 None:intergenic
GAAATTATCTGTGTGCCCAA+GGG 0.545744 2.3:-1928587 MS.gene86337:CDS
TTCATGAATACTTCCTACCT+AGG 0.553778 2.3:-1929193 MS.gene86337:CDS
CGAGTATATGGCATGGCGAA+AGG 0.557661 2.3:+1929646 None:intergenic
AGCATTTGGACTGTTTGGAT+TGG 0.564921 2.3:-1932410 MS.gene86337:CDS
CTTTCGCCATGCCATATACT+CGG 0.567046 2.3:-1929645 MS.gene86337:CDS
TGCATTCCGGAACAATATCT+GGG 0.570225 2.3:+1928770 None:intergenic
CTTAGGCTGCCCCATTCTAG+AGG 0.573194 2.3:-1929309 MS.gene86337:CDS
GAATTAAACTGTCAAAATTG+GGG 0.577717 2.3:+1929040 None:intergenic
AAACGCATATTCTCTACCCT+AGG 0.589888 2.3:+1929176 None:intergenic
TGCTTGTTTACAGGTTGCAG+AGG 0.594080 2.3:-1928727 MS.gene86337:CDS
AATTTATGAGGACAATTGGA+AGG 0.595790 2.3:-1928794 MS.gene86337:CDS
ATAGGCACTGTGAATTGTCA+TGG 0.596063 2.3:+1928645 None:intergenic
GCATATTCTCTACCCTAGGT+AGG 0.607522 2.3:+1929180 None:intergenic
TCATGAATACTTCCTACCTA+GGG 0.617474 2.3:-1929192 MS.gene86337:CDS
AAATTATCTGTGTGCCCAAG+GGG 0.625212 2.3:-1928586 MS.gene86337:CDS
GGTGTCGTCGCCGTCCTCGG+TGG 0.628299 2.3:-1931484 MS.gene86337:CDS
TGGGGACTAGTAAGTACATG+TGG 0.637068 2.3:+1929058 None:intergenic
GGAATGTAAAGAATGGTGGT+AGG 0.640379 2.3:-1932506 None:intergenic
TTTGCAATTCCTCTAGAATG+GGG 0.642459 2.3:+1929300 None:intergenic
CAAACAGTCCAAATGCTCGT+AGG 0.659699 2.3:+1932416 None:intergenic
CAATGACCGAGTATATGGCA+TGG 0.667166 2.3:+1929639 None:intergenic
ACAAGAACGTACCACCACCG+AGG 0.706622 2.3:+1931470 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAAGAAAAATATAGATT+TGG - chr2.3:1931033-1931052 MS.gene86337:intron 10.0%
!! TTTAGTGTAAAATAAAAATA+TGG - chr2.3:1931058-1931077 MS.gene86337:intron 10.0%
!!! AATAAATTATGGTTTTTTTT+TGG + chr2.3:1928847-1928866 None:intergenic 10.0%
!!! CTATTTTTCTTTAATTTAAA+AGG - chr2.3:1932049-1932068 MS.gene86337:intron 10.0%
!! AAAATATGGAGTATATAATA+TGG - chr2.3:1931072-1931091 MS.gene86337:intron 15.0%
!! ATAAAATAAATAATTCCTAG+TGG - chr2.3:1928895-1928914 MS.gene86337:intron 15.0%
!! ATAAAATATTTAATTGAGCA+AGG + chr2.3:1929166-1929185 None:intergenic 15.0%
!! ATAGTTCATTCAATAAATTA+TGG + chr2.3:1928858-1928877 None:intergenic 15.0%
!! TAATTTAAAAGGTAAAAGTT+GGG - chr2.3:1932060-1932079 MS.gene86337:intron 15.0%
!! TGTATATAACATTTACAATA+TGG - chr2.3:1930483-1930502 MS.gene86337:intron 15.0%
!! TTAAACTTAAGTTTGAATAA+TGG + chr2.3:1930386-1930405 None:intergenic 15.0%
!! TTAATTTAAAAGGTAAAAGT+TGG - chr2.3:1932059-1932078 MS.gene86337:intron 15.0%
!!! ATATTAATCTTTTTTTTGAG+AGG + chr2.3:1931103-1931122 None:intergenic 15.0%
!! AAAAAATATTATACACCACT+AGG + chr2.3:1928913-1928932 None:intergenic 20.0%
!! AAACTTAAGTTTAATGTTAG+AGG - chr2.3:1930392-1930411 MS.gene86337:intron 20.0%
!! AACTTAAGTTTAATGTTAGA+GGG - chr2.3:1930393-1930412 MS.gene86337:intron 20.0%
!! ATAAACATTATTTACCTTCA+TGG + chr2.3:1929289-1929308 None:intergenic 20.0%
!! CTCTATTTCAAAAACATATT+TGG - chr2.3:1930737-1930756 MS.gene86337:intron 20.0%
!! GTACTTTGCATTTAAATTTA+AGG + chr2.3:1932440-1932459 None:intergenic 20.0%
!! TAAAAAACAATAGTGAATAG+AGG + chr2.3:1930943-1930962 None:intergenic 20.0%
!! TGAATTAAACTGTCAAAATT+GGG + chr2.3:1932022-1932041 None:intergenic 20.0%
!! TGCATTTAAATTTAAGGAAT+AGG + chr2.3:1932434-1932453 None:intergenic 20.0%
!! TTAATCCTAAAACTAATCAA+AGG + chr2.3:1930326-1930345 None:intergenic 20.0%
!! TTCATTCTCTATGTTTAATT+TGG - chr2.3:1930189-1930208 MS.gene86337:intron 20.0%
!! TTGAATTAAACTGTCAAAAT+TGG + chr2.3:1932023-1932042 None:intergenic 20.0%
!! TTGACAAAAAAAAACACATT+TGG - chr2.3:1930883-1930902 MS.gene86337:intron 20.0%
!!! AAAAAAGTTTTTGCACTAAT+AGG - chr2.3:1929359-1929378 MS.gene86337:CDS 20.0%
!!! AAAAAGTTTTTGCACTAATA+GGG - chr2.3:1929360-1929379 MS.gene86337:CDS 20.0%
!!! ATTTTGGATTCAATATAGTA+AGG - chr2.3:1928688-1928707 MS.gene86337:CDS 20.0%
!!! TAGTGGTGTATAATATTTTT+TGG - chr2.3:1928912-1928931 MS.gene86337:intron 20.0%
!!! TTAGTTTTAGGATTAATTAG+AGG - chr2.3:1930330-1930349 MS.gene86337:intron 20.0%
!!! TTTTGACAGTTTAATTCAAT+TGG - chr2.3:1932024-1932043 MS.gene86337:intron 20.0%
!!! TTTTTGAAATAGAGAAGATT+AGG + chr2.3:1930731-1930750 None:intergenic 20.0%
! AAAAATAATCGACAAAGCAT+TGG + chr2.3:1930910-1930929 None:intergenic 25.0%
! AACGTGAGTTATTGAAAATT+TGG - chr2.3:1929691-1929710 MS.gene86337:intron 25.0%
! AGAGAATGAAGAAACTAAAT+CGG + chr2.3:1930179-1930198 None:intergenic 25.0%
! ATATCGTGGAAGATTTATTA+AGG + chr2.3:1930543-1930562 None:intergenic 25.0%
! ATATTGCATATTATTTGCAG+GGG - chr2.3:1930792-1930811 MS.gene86337:intron 25.0%
! ATGTGAGATATAATTGAATC+TGG + chr2.3:1931270-1931289 None:intergenic 25.0%
! CATATTTGTTTGATCTCAAA+TGG - chr2.3:1928784-1928803 MS.gene86337:CDS 25.0%
! GAATTAAACTGTCAAAATTG+GGG + chr2.3:1932021-1932040 None:intergenic 25.0%
! GATATTGCATATTATTTGCA+GGG - chr2.3:1930791-1930810 MS.gene86337:intron 25.0%
! GTATAGCTGCATTAATAAAA+CGG + chr2.3:1931521-1931540 None:intergenic 25.0%
! TAATTCACTTGATTGTTTAC+AGG - chr2.3:1932228-1932247 MS.gene86337:intron 25.0%
! TATGGATGACAAAATTGAAT+TGG - chr2.3:1930501-1930520 MS.gene86337:intron 25.0%
! TGCATAAACATGATATTTCA+AGG - chr2.3:1929201-1929220 MS.gene86337:CDS 25.0%
! TTGGATAATATGTTGTTCAA+TGG - chr2.3:1931702-1931721 MS.gene86337:intron 25.0%
!! AAAATAATCGACAAAGCATT+GGG + chr2.3:1930909-1930928 None:intergenic 25.0%
!! ATGAGGTATTACATATGTTT+AGG - chr2.3:1932149-1932168 MS.gene86337:intron 25.0%
!! CATTTGAGATCAAACAAATA+TGG + chr2.3:1928786-1928805 None:intergenic 25.0%
!!! TCATGAATATATTGACTTTC+AGG + chr2.3:1931852-1931871 None:intergenic 25.0%
!!! TTTTAGGATTAATTAGAGGA+TGG - chr2.3:1930334-1930353 MS.gene86337:intron 25.0%
!!! TTTTTTTGTCAATAGTCTAG+TGG + chr2.3:1930875-1930894 None:intergenic 25.0%
AAACAGAGAACTTTGAGTAT+AGG + chr2.3:1931684-1931703 None:intergenic 30.0%
AAACATGATATTTCAAGGAG+AGG - chr2.3:1929206-1929225 MS.gene86337:CDS 30.0%
AAAGCAATTGCTATGAACTA+GGG + chr2.3:1931166-1931185 None:intergenic 30.0%
AAGAGAATCTTGTCAATATC+TGG + chr2.3:1931732-1931751 None:intergenic 30.0%
AATGGTAAAATGTTGAAGCT+TGG + chr2.3:1932128-1932147 None:intergenic 30.0%
AATTTATGAGGACAATTGGA+AGG - chr2.3:1932264-1932283 MS.gene86337:intron 30.0%
AATTTATTTGTGGAGAAGTG+AGG + chr2.3:1930839-1930858 None:intergenic 30.0%
ACTAATAGGGTTGAGTATAA+CGG - chr2.3:1929373-1929392 MS.gene86337:CDS 30.0%
ACTGAAATATAATGCTCGAA+AGG - chr2.3:1929896-1929915 MS.gene86337:intron 30.0%
ATAAGCCATATACTCGAAAA+AGG + chr2.3:1930589-1930608 None:intergenic 30.0%
ATATTTCAGTCCAAAGATGA+AGG + chr2.3:1929886-1929905 None:intergenic 30.0%
ATGCAAAGTACGAAATGTTA+CGG - chr2.3:1932449-1932468 MS.gene86337:CDS 30.0%
ATGGAATTTATGAGGACAAT+TGG - chr2.3:1932260-1932279 MS.gene86337:intron 30.0%
ATGGTAAAATGTTGAAGCTT+GGG + chr2.3:1932127-1932146 None:intergenic 30.0%
ATTAATGCAGCTATACAAAG+AGG - chr2.3:1931525-1931544 MS.gene86337:intron 30.0%
CAAAGATACATCGATAATAG+AGG + chr2.3:1932205-1932224 None:intergenic 30.0%
CATATGTAATACCTCATGAA+TGG + chr2.3:1932146-1932165 None:intergenic 30.0%
CATATTCTATGCCAAATTTG+AGG + chr2.3:1931482-1931501 None:intergenic 30.0%
CATCGAAAATGGAATTTATG+AGG - chr2.3:1932252-1932271 MS.gene86337:intron 30.0%
CTGAAATATAATGCTCGAAA+GGG - chr2.3:1929897-1929916 MS.gene86337:intron 30.0%
CTTATATTGAGGATGTGATT+CGG - chr2.3:1930604-1930623 MS.gene86337:intron 30.0%
GAGTTATTGAAAATTTGGCT+AGG - chr2.3:1929696-1929715 MS.gene86337:intron 30.0%
GATATTGACAAGATTCTCTT+AGG - chr2.3:1931732-1931751 MS.gene86337:intron 30.0%
GGATATGATTAATGATATCG+TGG + chr2.3:1930557-1930576 None:intergenic 30.0%
GGATATTGCATATTATTTGC+AGG - chr2.3:1930790-1930809 MS.gene86337:intron 30.0%
GTTTCGTCTATACAGAAATT+GGG - chr2.3:1931937-1931956 MS.gene86337:intron 30.0%
TATACTCAAAGTTCTCTGTT+TGG - chr2.3:1931683-1931702 MS.gene86337:intron 30.0%
TGTTATTAAAACGACAATGC+TGG + chr2.3:1930644-1930663 None:intergenic 30.0%
TGTTTCGTCTATACAGAAAT+TGG - chr2.3:1931936-1931955 MS.gene86337:intron 30.0%
TTGTTCTTGTTCACTGTTAT+TGG - chr2.3:1929852-1929871 MS.gene86337:intron 30.0%
TTTGAGAAGAATGAACTTGA+AGG - chr2.3:1928612-1928631 MS.gene86337:CDS 30.0%
! AGAGAATATGCGTTTAAAAC+TGG - chr2.3:1931890-1931909 MS.gene86337:intron 30.0%
! ATTTTGAATCACTTCTCTGT+TGG - chr2.3:1930138-1930157 MS.gene86337:intron 30.0%
! TCAACATTTTACCATTCATG+AGG - chr2.3:1932132-1932151 MS.gene86337:intron 30.0%
! TTTCAATAACTCACGTTTTG+TGG + chr2.3:1929688-1929707 None:intergenic 30.0%
!! AATACTTGTTATCGCCAAAT+TGG + chr2.3:1931829-1931848 None:intergenic 30.0%
!! ATACTTGTTATCGCCAAATT+GGG + chr2.3:1931828-1931847 None:intergenic 30.0%
!!! GAACACCTTTGATTAGTTTT+AGG - chr2.3:1930318-1930337 MS.gene86337:intron 30.0%
AAAGGTGATGTAGTCTTTGA+AGG + chr2.3:1931397-1931416 None:intergenic 35.0%
AACAAAGAGAGAAGATCTGA+CGG - chr2.3:1929035-1929054 MS.gene86337:CDS 35.0%
AATTGAGCAAGGAAATGTCT+CGG + chr2.3:1929155-1929174 None:intergenic 35.0%
AATTGTCGTCTTTCACAGAT+AGG + chr2.3:1931647-1931666 None:intergenic 35.0%
ACAGTGAACAAGAACAAACT+CGG + chr2.3:1929849-1929868 None:intergenic 35.0%
AGCTGCATTAATAAAACGGT+TGG + chr2.3:1931517-1931536 None:intergenic 35.0%
ATCAAACAAATATGGACGAC+GGG + chr2.3:1928778-1928797 None:intergenic 35.0%
ATCCATTCCAACAGAAGATA+GGG - chr2.3:1931233-1931252 MS.gene86337:intron 35.0%
ATGAAGAAGCTCAAGATTAG+CGG - chr2.3:1929070-1929089 MS.gene86337:intron 35.0%
CAAAGCAATTGCTATGAACT+AGG + chr2.3:1931167-1931186 None:intergenic 35.0%
CAACACACATTGAATTGCAA+TGG + chr2.3:1930028-1930047 None:intergenic 35.0%
CATGGCATTAACATCATCAA+AGG + chr2.3:1929232-1929251 None:intergenic 35.0%
CCATATACTCGAAAAAGGAA+TGG + chr2.3:1930584-1930603 None:intergenic 35.0%
CGAGTATATGGCTTATATTG+AGG - chr2.3:1930593-1930612 MS.gene86337:intron 35.0%
GACATGTTCGAGAAGTTTAA+TGG - chr2.3:1931567-1931586 MS.gene86337:intron 35.0%
GAGCTAATCTCTCAAAAAGA+AGG - chr2.3:1928992-1929011 MS.gene86337:CDS 35.0%
GAGCTCACACAATTTATTTG+TGG + chr2.3:1930849-1930868 None:intergenic 35.0%
GATCAAACAAATATGGACGA+CGG + chr2.3:1928779-1928798 None:intergenic 35.0%
GATGATGTTAATGCCATGAA+GGG - chr2.3:1929234-1929253 MS.gene86337:CDS 35.0%
GCATCACTGAAAAAAGAAAC+AGG - chr2.3:1930415-1930434 MS.gene86337:intron 35.0%
GGATGACAAAATTGAATTGG+AGG - chr2.3:1930504-1930523 MS.gene86337:intron 35.0%
GTAAAAGTTGGGATAAGAAG+TGG - chr2.3:1932071-1932090 MS.gene86337:intron 35.0%
GTCTATACAGAAATTGGGTA+TGG - chr2.3:1931942-1931961 MS.gene86337:intron 35.0%
TACATGGTGGTCGAAAATAA+AGG + chr2.3:1929480-1929499 None:intergenic 35.0%
TCATGAATACTTCCTACCTA+GGG - chr2.3:1931866-1931885 MS.gene86337:intron 35.0%
TGATACAATGACCGAGTATA+TGG + chr2.3:1931427-1931446 None:intergenic 35.0%
TGCAGATTTCATGGCTAATT+CGG + chr2.3:1929101-1929120 None:intergenic 35.0%
TGTTTACAGGACATCGAAAA+TGG - chr2.3:1932241-1932260 MS.gene86337:intron 35.0%
TTCATGAATACTTCCTACCT+AGG - chr2.3:1931865-1931884 MS.gene86337:intron 35.0%
TTCCTCCAAATCAAAACAGT+GGG + chr2.3:1929974-1929993 None:intergenic 35.0%
TTGCAATTCAATGTGTGTTG+TGG - chr2.3:1930028-1930047 MS.gene86337:intron 35.0%
TTGGAGGAACATGTATGATT+TGG - chr2.3:1929985-1930004 MS.gene86337:intron 35.0%
! TGATGATGTTAATGCCATGA+AGG - chr2.3:1929233-1929252 MS.gene86337:CDS 35.0%
! TGTTTGCAATTCCTCTAGAA+TGG + chr2.3:1931763-1931782 None:intergenic 35.0%
!! CTATGTTTAATTTGGACCGT+AGG - chr2.3:1930197-1930216 MS.gene86337:intron 35.0%
!! GTTTGCAATTCCTCTAGAAT+GGG + chr2.3:1931762-1931781 None:intergenic 35.0%
!! TCAGATGGTTTGACTTGAAA+CGG - chr2.3:1930077-1930096 MS.gene86337:intron 35.0%
!! TTTGCAATTCCTCTAGAATG+GGG + chr2.3:1931761-1931780 None:intergenic 35.0%
!!! ATCGATTTCTGTTGTTTTGC+AGG - chr2.3:1930105-1930124 MS.gene86337:intron 35.0%
!!! CCATTCCTTTTTCGAGTATA+TGG - chr2.3:1930581-1930600 MS.gene86337:intron 35.0%
AAACGCATATTCTCTACCCT+AGG + chr2.3:1931885-1931904 None:intergenic 40.0%
AAATTATCTGTGTGCCCAAG+GGG - chr2.3:1932472-1932491 MS.gene86337:CDS 40.0%
AACCCTATCTTCTGTTGGAA+TGG + chr2.3:1931238-1931257 None:intergenic 40.0%
AATATAAGTTCACAGCCCCT+TGG + chr2.3:1932490-1932509 None:intergenic 40.0%
ACGTCGTGTTCTATTTCAGA+TGG - chr2.3:1930062-1930081 MS.gene86337:intron 40.0%
ACTCGAAAAAGGAATGGAAG+AGG + chr2.3:1930578-1930597 None:intergenic 40.0%
AGCATTTGGACTGTTTGGAT+TGG - chr2.3:1928648-1928667 MS.gene86337:CDS 40.0%
AGCTGAGATGAAATGCATTC+CGG + chr2.3:1932304-1932323 None:intergenic 40.0%
AGGTGTTCAGTGTTGTGATA+GGG + chr2.3:1930306-1930325 None:intergenic 40.0%
ATATAAGTTCACAGCCCCTT+GGG + chr2.3:1932489-1932508 None:intergenic 40.0%
ATCAATGTTCTAACTCCGAC+TGG - chr2.3:1929424-1929443 MS.gene86337:CDS 40.0%
ATGCATTCCGGAACAATATC+TGG + chr2.3:1932292-1932311 None:intergenic 40.0%
ATGTTCTCTCAGTTTCGATC+TGG - chr2.3:1929721-1929740 MS.gene86337:intron 40.0%
CAGATCTTCTCTCTTTGTTG+CGG + chr2.3:1929034-1929053 None:intergenic 40.0%
CAGTCCAAAGATGAAGGATT+TGG + chr2.3:1929880-1929899 None:intergenic 40.0%
CGATCATTTCGCCTCAAATT+TGG - chr2.3:1931468-1931487 MS.gene86337:intron 40.0%
CTTGCAACTAAAAATGCGAC+TGG + chr2.3:1931598-1931617 None:intergenic 40.0%
GAAATTATCTGTGTGCCCAA+GGG - chr2.3:1932471-1932490 MS.gene86337:CDS 40.0%
GAACCAATGTGCAGATTTCA+TGG + chr2.3:1929110-1929129 None:intergenic 40.0%
GATCCATTCCAACAGAAGAT+AGG - chr2.3:1931232-1931251 MS.gene86337:intron 40.0%
GATGAAACTGTTCGTCAACA+CGG + chr2.3:1929950-1929969 None:intergenic 40.0%
GGTTCCAAATCCTTCATCTT+TGG - chr2.3:1929873-1929892 MS.gene86337:intron 40.0%
GTTCCTCCAAATCAAAACAG+TGG + chr2.3:1929975-1929994 None:intergenic 40.0%
GTTTCATTCCTACGAGCATT+TGG - chr2.3:1928634-1928653 MS.gene86337:CDS 40.0%
TAACACCTTAGAGCTTGAGA+GGG - chr2.3:1930444-1930463 MS.gene86337:intron 40.0%
TACCAACTGAACTAAGCTCA+CGG + chr2.3:1930769-1930788 None:intergenic 40.0%
TAGCCATGAAATCTGCACAT+TGG - chr2.3:1929104-1929123 MS.gene86337:intron 40.0%
TCAATGTTCTAACTCCGACT+GGG - chr2.3:1929425-1929444 MS.gene86337:CDS 40.0%
TCTCAGTTTCGATCTGGATA+TGG - chr2.3:1929727-1929746 MS.gene86337:intron 40.0%
TGCATTCCGGAACAATATCT+GGG + chr2.3:1932291-1932310 None:intergenic 40.0%
TTAACACCTTAGAGCTTGAG+AGG - chr2.3:1930443-1930462 MS.gene86337:intron 40.0%
TTATCCATACCCTTCGTGAA+GGG + chr2.3:1929132-1929151 None:intergenic 40.0%
TTGAGGCGAAATGATCGTAT+TGG + chr2.3:1931465-1931484 None:intergenic 40.0%
! AAGGTGTTCAGTGTTGTGAT+AGG + chr2.3:1930307-1930326 None:intergenic 40.0%
! ACACACAAGTGAAGCTTTTG+AGG - chr2.3:1930998-1931017 MS.gene86337:intron 40.0%
! ATAGGCACTGTGAATTGTCA+TGG + chr2.3:1932416-1932435 None:intergenic 40.0%
! ATAGGGTTGAGTATAACGGA+AGG - chr2.3:1929377-1929396 MS.gene86337:CDS 40.0%
! CTACGAACTTGCTTGTTTAC+AGG - chr2.3:1932322-1932341 MS.gene86337:intron 40.0%
! GATGTAGTCTTTGAAGGTCT+GGG + chr2.3:1931391-1931410 None:intergenic 40.0%
! TCTGAAATAGAACACGACGT+CGG + chr2.3:1930062-1930081 None:intergenic 40.0%
! TGAGCTTAGTTCAGTTGGTA+GGG - chr2.3:1930769-1930788 MS.gene86337:intron 40.0%
! TGATGTAGTCTTTGAAGGTC+TGG + chr2.3:1931392-1931411 None:intergenic 40.0%
!! AATTTGCTGCAGGAAGTTGA+TGG - chr2.3:1930249-1930268 MS.gene86337:intron 40.0%
!! ACTGAGCTAGTTTGCTATTC+CGG + chr2.3:1929262-1929281 None:intergenic 40.0%
!!! CATCTCCCACTGTTTTGATT+TGG - chr2.3:1929966-1929985 MS.gene86337:intron 40.0%
AAAGAGAGAAGATCTGACGG+AGG - chr2.3:1929038-1929057 MS.gene86337:CDS 45.0%
AAGAGAGAAGATCTGACGGA+GGG - chr2.3:1929039-1929058 MS.gene86337:CDS 45.0%
AAGGAAGAATCCCGTCGTAA+TGG - chr2.3:1928707-1928726 MS.gene86337:CDS 45.0%
AATGGATCGATGAGGTTGAG+AGG + chr2.3:1931220-1931239 None:intergenic 45.0%
AATGTTCTAACTCCGACTGG+GGG - chr2.3:1929427-1929446 MS.gene86337:CDS 45.0%
ACCAACTGAACTAAGCTCAC+GGG + chr2.3:1930768-1930787 None:intergenic 45.0%
ACCAATGACCACCATTACGA+CGG + chr2.3:1928721-1928740 None:intergenic 45.0%
CAAACAGTCCAAATGCTCGT+AGG + chr2.3:1928645-1928664 None:intergenic 45.0%
CAAAGCGGTGGAATCCATTA+TGG - chr2.3:1931337-1931356 MS.gene86337:intron 45.0%
CAATGACCGAGTATATGGCA+TGG + chr2.3:1931422-1931441 None:intergenic 45.0%
CAATGTTCTAACTCCGACTG+GGG - chr2.3:1929426-1929445 MS.gene86337:CDS 45.0%
CTACGAGCATTTGGACTGTT+TGG - chr2.3:1928643-1928662 MS.gene86337:CDS 45.0%
CTTTCGCCATGCCATATACT+CGG - chr2.3:1931413-1931432 MS.gene86337:intron 45.0%
GAAGGACCCAGATATTGTTC+CGG - chr2.3:1932282-1932301 MS.gene86337:intron 45.0%
GAGCTAACCCTATCTTCTGT+TGG + chr2.3:1931243-1931262 None:intergenic 45.0%
GCATATTCTCTACCCTAGGT+AGG + chr2.3:1931881-1931900 None:intergenic 45.0%
GGAAATTATCTGTGTGCCCA+AGG - chr2.3:1932470-1932489 MS.gene86337:CDS 45.0%
GTGAGCTTAGTTCAGTTGGT+AGG - chr2.3:1930768-1930787 MS.gene86337:intron 45.0%
GTGGAATCCATTATGGCTCT+CGG - chr2.3:1931344-1931363 MS.gene86337:intron 45.0%
GTTATCCATACCCTTCGTGA+AGG + chr2.3:1929133-1929152 None:intergenic 45.0%
GTTGGGATAAGAAGTGGAAG+TGG - chr2.3:1932077-1932096 MS.gene86337:intron 45.0%
TAATGCTCGAAAGGGCTTTG+TGG - chr2.3:1929905-1929924 MS.gene86337:intron 45.0%
TACTCCAGAAACGCCCAATT+TGG - chr2.3:1931812-1931831 MS.gene86337:intron 45.0%
TCTGTTGGAATGGATCGATG+AGG + chr2.3:1931228-1931247 None:intergenic 45.0%
TGCATATTATTTGCAGGGGC+CGG - chr2.3:1930796-1930815 MS.gene86337:intron 45.0%
TGCTTGTTTACAGGTTGCAG+AGG - chr2.3:1932331-1932350 MS.gene86337:intron 45.0%
TGGTCTCTTAAAACCACCGT+CGG - chr2.3:1929747-1929766 MS.gene86337:intron 45.0%
TGGTGGTAGGAAGAGAAGAA+AGG - chr2.3:1928565-1928584 MS.gene86337:CDS 45.0%
TTGTTTGATCACAGAGTGCG+AGG - chr2.3:1928749-1928768 MS.gene86337:CDS 45.0%
! GTGAGCTTGCAGCAGAATTT+TGG - chr2.3:1928672-1928691 MS.gene86337:CDS 45.0%
! TGGGGACTAGTAAGTACATG+TGG + chr2.3:1932003-1932022 None:intergenic 45.0%
!! ACATTGGTTCCCTTCACGAA+GGG - chr2.3:1929120-1929139 MS.gene86337:intron 45.0%
!! CTTGCTGTCAAATTTGCTGC+AGG - chr2.3:1930239-1930258 MS.gene86337:intron 45.0%
!! TGCAGGAAGTTGATGGTACA+AGG - chr2.3:1930256-1930275 MS.gene86337:intron 45.0%
!!! CTCCCACTGTTTTGATTTGG+AGG - chr2.3:1929969-1929988 MS.gene86337:intron 45.0%
AACCTAGAAACTGACCACCG+AGG + chr2.3:1929778-1929797 None:intergenic 50.0%
AATAGACCCCCGAAGGAGTT+TGG + chr2.3:1929548-1929567 None:intergenic 50.0%
ACCAGTAGGAAAGTGGCGAT+AGG + chr2.3:1929806-1929825 None:intergenic 50.0%
AGAGAGAAGATCTGACGGAG+GGG - chr2.3:1929040-1929059 MS.gene86337:CDS 50.0%
AGGACTCGCTTTCGTGTTGA+CGG - chr2.3:1930276-1930295 MS.gene86337:intron 50.0%
ATATGAACATCGCCCCCAGT+CGG + chr2.3:1929442-1929461 None:intergenic 50.0%
ATCGCCAAATTGGGCGTTTC+TGG + chr2.3:1931819-1931838 None:intergenic 50.0%
CATATTATTTGCAGGGGCCG+GGG - chr2.3:1930798-1930817 MS.gene86337:intron 50.0%
CCAACTGAACTAAGCTCACG+GGG + chr2.3:1930767-1930786 None:intergenic 50.0%
CCAATGACCACCATTACGAC+GGG + chr2.3:1928720-1928739 None:intergenic 50.0%
CGAGTATATGGCATGGCGAA+AGG + chr2.3:1931415-1931434 None:intergenic 50.0%
CGTAATGGTGGTCATTGGTG+TGG - chr2.3:1928722-1928741 MS.gene86337:CDS 50.0%
CGTCATCACCAAACTCCTTC+GGG - chr2.3:1929537-1929556 MS.gene86337:CDS 50.0%
CTAGCTCAGTGCTACCATGA+AGG - chr2.3:1929272-1929291 MS.gene86337:CDS 50.0%
GAAGAATCCCGTCGTAATGG+TGG - chr2.3:1928710-1928729 MS.gene86337:CDS 50.0%
GCATATTATTTGCAGGGGCC+GGG - chr2.3:1930797-1930816 MS.gene86337:intron 50.0%
GCGTCATCACCAAACTCCTT+CGG - chr2.3:1929536-1929555 MS.gene86337:CDS 50.0%
GTCATCACCAAACTCCTTCG+GGG - chr2.3:1929538-1929557 MS.gene86337:CDS 50.0%
GTTAATGCCATGAAGGGAGC+CGG - chr2.3:1929240-1929259 MS.gene86337:CDS 50.0%
TCATCACCAAACTCCTTCGG+GGG - chr2.3:1929539-1929558 MS.gene86337:CDS 50.0%
TCCATCACTATCCTTCGCGT+CGG - chr2.3:1929620-1929639 MS.gene86337:CDS 50.0%
TCGAGAACCGAGAGCCATAA+TGG + chr2.3:1931354-1931373 None:intergenic 50.0%
TTGGACCGTAGGACTCGAAA+GGG - chr2.3:1930208-1930227 MS.gene86337:intron 50.0%
TTTCGACCACCATGTAGCCA+CGG - chr2.3:1929484-1929503 MS.gene86337:CDS 50.0%
TTTGGACCGTAGGACTCGAA+AGG - chr2.3:1930207-1930226 MS.gene86337:intron 50.0%
! AACGACCCTTTCGAGTCCTA+CGG + chr2.3:1930216-1930235 None:intergenic 50.0%
! ACCTATCGCCACTTTCCTAC+TGG - chr2.3:1929802-1929821 MS.gene86337:intron 50.0%
! ATGGATTCCACCGCTTTGAG+AGG + chr2.3:1931335-1931354 None:intergenic 50.0%
! GAGGATGTGATTCGGCGATA+TGG - chr2.3:1930612-1930631 MS.gene86337:intron 50.0%
! TATCGCCACTTTCCTACTGG+TGG - chr2.3:1929805-1929824 MS.gene86337:intron 50.0%
! TGGCAACGGAGTGTTTGGTT+GGG + chr2.3:1931305-1931324 None:intergenic 50.0%
!! CACATTGGTTCCCTTCACGA+AGG - chr2.3:1929119-1929138 MS.gene86337:intron 50.0%
ACAAGAACGTACCACCACCG+AGG + chr2.3:1929591-1929610 None:intergenic 55.0%
CACCTCCACCAGTAGGAAAG+TGG + chr2.3:1929813-1929832 None:intergenic 55.0%
CACTTTCCTACTGGTGGAGG+TGG - chr2.3:1929811-1929830 MS.gene86337:intron 55.0%
CATCACTATCCTTCGCGTCG+GGG - chr2.3:1929622-1929641 MS.gene86337:CDS 55.0%
CCACAAGCGTCCTCTCAAAG+CGG - chr2.3:1931322-1931341 MS.gene86337:intron 55.0%
CCATCACTATCCTTCGCGTC+GGG - chr2.3:1929621-1929640 MS.gene86337:CDS 55.0%
CCCCGTGAGCTTAGTTCAGT+TGG - chr2.3:1930764-1930783 MS.gene86337:intron 55.0%
CCCGTCGTAATGGTGGTCAT+TGG - chr2.3:1928717-1928736 MS.gene86337:CDS 55.0%
CGAGTCCCTCTCAAGCTCTA+AGG + chr2.3:1930452-1930471 None:intergenic 55.0%
CTAGAAACTGACCACCGAGG+CGG + chr2.3:1929775-1929794 None:intergenic 55.0%
CTGAACTAAGCTCACGGGGA+TGG + chr2.3:1930763-1930782 None:intergenic 55.0%
CTGGAGAAATAGACCCCCGA+AGG + chr2.3:1929555-1929574 None:intergenic 55.0%
CTTAGGCTGCCCCATTCTAG+AGG - chr2.3:1931749-1931768 MS.gene86337:intron 55.0%
GACGCGAAGGATAGTGATGG+AGG + chr2.3:1929621-1929640 None:intergenic 55.0%
GGTTCCCTTCACGAAGGGTA+TGG - chr2.3:1929125-1929144 MS.gene86337:intron 55.0%
TGAGAGGACGCTTGTGGCAA+CGG + chr2.3:1931319-1931338 None:intergenic 55.0%
TGCTATTCCGGCTCCCTTCA+TGG + chr2.3:1929250-1929269 None:intergenic 55.0%
TTCGACCACCATGTAGCCAC+GGG - chr2.3:1929485-1929504 MS.gene86337:CDS 55.0%
TTGTTGCGGAGTGATGCAGC+TGG + chr2.3:1929020-1929039 None:intergenic 55.0%
! CTTCGGGGGTCTATTTCTCC+AGG - chr2.3:1929553-1929572 MS.gene86337:CDS 55.0%
! GCTTGTGGCAACGGAGTGTT+TGG + chr2.3:1931310-1931329 None:intergenic 55.0%
! GTGGAGAAGTGAGGTGTCCA+GGG + chr2.3:1930830-1930849 None:intergenic 55.0%
! GTGGCAACGGAGTGTTTGGT+TGG + chr2.3:1931306-1931325 None:intergenic 55.0%
! TGCTCGAAAGGGCTTTGTGG+TGG - chr2.3:1929908-1929927 MS.gene86337:intron 55.0%
! TGTGGAGAAGTGAGGTGTCC+AGG + chr2.3:1930831-1930850 None:intergenic 55.0%
!! AGGGCTTTGTGGTGGATTCG+AGG - chr2.3:1929916-1929935 MS.gene86337:intron 55.0%
!! GTTTGGTGATGACGCAACCG+TGG + chr2.3:1929531-1929550 None:intergenic 55.0%
ATCAACAGTCCCCGACGCGA+AGG + chr2.3:1929634-1929653 None:intergenic 60.0%
CAAGCGTCCTCTCAAAGCGG+TGG - chr2.3:1931325-1931344 MS.gene86337:intron 60.0%
CCAGAACCACCTCCACCAGT+AGG + chr2.3:1929820-1929839 None:intergenic 60.0%
CCCGACGCGAAGGATAGTGA+TGG + chr2.3:1929624-1929643 None:intergenic 60.0%
CCTACTGGTGGAGGTGGTTC+TGG - chr2.3:1929817-1929836 MS.gene86337:intron 60.0%
CGCCTCGGTGGTCAGTTTCT+AGG - chr2.3:1929773-1929792 MS.gene86337:intron 60.0%
GAACGTACCACCACCGAGGA+CGG + chr2.3:1929587-1929606 None:intergenic 60.0%
GGTGATGCGATCAGCCTCCA+CGG - chr2.3:1929511-1929530 MS.gene86337:CDS 60.0%
TCACCATCCCCGTGGCTACA+TGG + chr2.3:1929496-1929515 None:intergenic 60.0%
TCGACCACCATGTAGCCACG+GGG - chr2.3:1929486-1929505 MS.gene86337:CDS 60.0%
TGATCGCATCACCATCCCCG+TGG + chr2.3:1929504-1929523 None:intergenic 60.0%
! CGCCACTTTCCTACTGGTGG+AGG - chr2.3:1929808-1929827 MS.gene86337:intron 60.0%
! TGGTGATGACGCAACCGTGG+AGG + chr2.3:1929528-1929547 None:intergenic 60.0%
!! CCGCTTTGAGAGGACGCTTG+TGG + chr2.3:1931325-1931344 None:intergenic 60.0%
CCACCATGTAGCCACGGGGA+TGG - chr2.3:1929490-1929509 MS.gene86337:CDS 65.0%
CCATCCCCGTGGCTACATGG+TGG + chr2.3:1929493-1929512 None:intergenic 65.0%
GGTGTCCAGGGTTCGAACCC+CGG + chr2.3:1930818-1930837 None:intergenic 65.0%
AACCACCGTCGGAGCCGCCT+CGG - chr2.3:1929758-1929777 MS.gene86337:intron 70.0%
! CCAGGTGTCGTCGCCGTCCT+CGG - chr2.3:1929571-1929590 MS.gene86337:CDS 70.0%
AGGGGCCGGGGTTCGAACCC+TGG - chr2.3:1930810-1930829 MS.gene86337:intron 75.0%
CCGAGGACGGCGACGACACC+TGG + chr2.3:1929574-1929593 None:intergenic 75.0%
GACCACCGAGGCGGCTCCGA+CGG + chr2.3:1929766-1929785 None:intergenic 75.0%
GTCGTCGCCGTCCTCGGTGG+TGG - chr2.3:1929577-1929596 MS.gene86337:CDS 75.0%
! GGTGTCGTCGCCGTCCTCGG+TGG - chr2.3:1929574-1929593 MS.gene86337:CDS 75.0%
! CACCGAGGCGGCTCCGACGG+TGG + chr2.3:1929763-1929782 None:intergenic 80.0%
! CACCGTCGGAGCCGCCTCGG+TGG - chr2.3:1929761-1929780 MS.gene86337:intron 80.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 1928564 1932516 1928564 ID=MS.gene86337
chr2.3 mRNA 1928564 1932516 1928564 ID=MS.gene86337.t1;Parent=MS.gene86337
chr2.3 exon 1932376 1932516 1932376 ID=MS.gene86337.t1.exon1;Parent=MS.gene86337.t1
chr2.3 CDS 1932376 1932516 1932376 ID=cds.MS.gene86337.t1;Parent=MS.gene86337.t1
chr2.3 exon 1931482 1931505 1931482 ID=MS.gene86337.t1.exon2;Parent=MS.gene86337.t1
chr2.3 CDS 1931482 1931505 1931482 ID=cds.MS.gene86337.t1;Parent=MS.gene86337.t1
chr2.3 exon 1929169 1929687 1929169 ID=MS.gene86337.t1.exon3;Parent=MS.gene86337.t1
chr2.3 CDS 1929169 1929687 1929169 ID=cds.MS.gene86337.t1;Parent=MS.gene86337.t1
chr2.3 exon 1928927 1929085 1928927 ID=MS.gene86337.t1.exon4;Parent=MS.gene86337.t1
chr2.3 CDS 1928927 1929085 1928927 ID=cds.MS.gene86337.t1;Parent=MS.gene86337.t1
chr2.3 exon 1928564 1928830 1928564 ID=MS.gene86337.t1.exon5;Parent=MS.gene86337.t1
chr2.3 CDS 1928564 1928830 1928564 ID=cds.MS.gene86337.t1;Parent=MS.gene86337.t1
Gene Sequence

>MS.gene86337

ATGGTGGTAGGAAGAGAAGAAAGGAGTTTGTTTCTTGACAGATCAATGTTTGAGAAGAATGAACTTGAAGGTTTCATTCCTACGAGCATTTGGACTGTTTGGATTGGTGTGAGCTTGCAGCAGAATTTTGGATTCAATATAGTAAGGAAGAATCCCGTCGTAATGGTGGTCATTGGTGTGGCGTCTTGTTTGATCACAGAGTGCGAGGAATCCCGTCGTCCATATTTGTTTGATCTCAAATGGTCAACTTAATTTTTTATTTTTTTATTAACAATGAAAGCCAAAAAAAAACCATAATTTATTGAATGAACTATGAACATCTACATAAAATATAAAATAAATAATTCCTAGTGGTGTATAATATTTTTTGGTTACAATGCTAAGAAAAGAACAAGAAAAAAGACGACAACAGAAGAAAAAAAGCAACAGAGCTAATCTCTCAAAAAGAAGGTTCCAGCTGCATCACTCCGCAACAAAGAGAGAAGATCTGACGGAGGGGAGACTTGATGAAGAAGCTCAAGATTAGCGGAAACACCGAATTAGCCATGAAATCTGCACATTGGTTCCCTTCACGAAGGGTATGGATAACCGAGACATTTCCTTGCTCAATTAAATATTTTATATCTTGAATCAAAACTGCATAAACATGATATTTCAAGGAGAGGCCTTTGATGATGTTAATGCCATGAAGGGAGCCGGAATAGCAAACTAGCTCAGTGCTACCATGAAGGTAAATAATGTTTATATTAAATATTATTTTCAACACATATTATTGATTAAACTAAATTGTTAATCAAAAAAGTTTTTGCACTAATAGGGTTGAGTATAACGGAAGGATGTAAACTCTCACAGACTGACGAATCAATGTTCTAACTCCGACTGGGGGCGATGTTCATATTTAGTGTTTGACATGCCTTTATTTTCGACCACCATGTAGCCACGGGGATGGTGATGCGATCAGCCTCCACGGTTGCGTCATCACCAAACTCCTTCGGGGGTCTATTTCTCCAGGTGTCGTCGCCGTCCTCGGTGGTGGTACGTTCTTGTTCTTAGACCTCCATCACTATCCTTCGCGTCGGGGACTGTTGATTTGCTGAAAACATGTTTGTGTGTGTGTTTCACCACAAAACGTGAGTTATTGAAAATTTGGCTAGGATATGTTCTCTCAGTTTCGATCTGGATATGGTCTCTTAAAACCACCGTCGGAGCCGCCTCGGTGGTCAGTTTCTAGGTTTACAACCTATCGCCACTTTCCTACTGGTGGAGGTGGTTCTGGTTCTGTCCGAGTTTGTTCTTGTTCACTGTTATTGGTTCCAAATCCTTCATCTTTGGACTGAAATATAATGCTCGAAAGGGCTTTGTGGTGGATTCGAGGACTTGTTTCCGTGTTGACGAACAGTTTCATCTCCCACTGTTTTGATTTGGAGGAACATGTATGATTTGGAGTTGAACGCTCTGATACCATTGCAATTCAATGTGTGTTGTGGTTTGTGTACCGACGTCGTGTTCTATTTCAGATGGTTTGACTTGAAACGGCAATTATCGATTTCTGTTGTTTTGCAGGAAGTTGATAGATTTTGAATCACTTCTCTGTTGGCAGACGATTCATACGCCGATTTAGTTTCTTCATTCTCTATGTTTAATTTGGACCGTAGGACTCGAAAGGGTCGTTTTGCTTGCTGTCAAATTTGCTGCAGGAAGTTGATGGTACAAGGACTCGCTTTCGTGTTGACGGACGACCCTATCACAACACTGAACACCTTTGATTAGTTTTAGGATTAATTAGAGGATGGTATTTTTTATTTTTAATTTTCATTATCCATTATTCAAACTTAAGTTTAATGTTAGAGGGCATCACTGAAAAAAGAAACAGGAATCGTTAACACCTTAGAGCTTGAGAGGGACTCGTTTGTTTTCTCTGTATATAACATTTACAATATGGATGACAAAATTGAATTGGAGGTTGTTTAAATTTTCCTTAATAAATCTTCCACGATATCATTAATCATATCCTCTTCCATTCCTTTTTCGAGTATATGGCTTATATTGAGGATGTGATTCGGCGATATGGCGTTAGCCAGCATTGTCGTTTTAATAACAAAAGTGAAATCATAATTATTTTTCGATTTTTTTTTGAAAGAATAATTATTTTTTGAAACAAAGTCCTAATCTTCTCTATTTCAAAAACATATTTGGCCATCCCCGTGAGCTTAGTTCAGTTGGTAGGGATATTGCATATTATTTGCAGGGGCCGGGGTTCGAACCCTGGACACCTCACTTCTCCACAAATAAATTGTGTGAGCTCTAGCCACTAGACTATTGACAAAAAAAAACACATTTGGCCCAATGCTTTGTCGATTATTTTTCATTTGTACTCCTCTATTCACTATTGTTTTTTAGATAGATAAAATGACAATAATCATAAAAAATTTACACACACAAGTGAAGCTTTTGAGGTATTAATTTTTATTTAAAGAAAAATATAGATTTGGTTTTTAGTGTAAAATAAAAATATGGAGTATATAATATGGAATATCCTCTCAAAAAAAAGATTAATATTTTTTTAAAAACTAAAAAGATGAATATATTTGCATCAAAACCCTAGTTCATAGCAATTGCTTTGATCTTAATTTTGCAATTGTGATCATGTCTTCCTCTCAACCTCATCGATCCATTCCAACAGAAGATAGGGTTAGCTCTCTTCCAGATTCAATTATATCTCACATTCTCTCTTTTCTCCCAACCAAACACTCCGTTGCCACAAGCGTCCTCTCAAAGCGGTGGAATCCATTATGGCTCTCGGTTCTCGATCTCAACTTTGATGCCCAGACCTTCAAAGACTACATCACCTTTCGCCATGCCATATACTCGGTCATTGTATCACGAGACAACACGCTGCCAATACGATCATTTCGCCTCAAATTTGGCATAGAATATGACATCAACGACGCCAACCGTTTTATTAATGCAGCTATACAAAGAGGAATTGAAAAGCTAGACATTGACATGTTCGAGAAGTTTAATGGATTAACCAGTCGCATTTTTAGTTGCAAGACACTCACTGTTCTCAAGTTGAAAAACCTATCTGTGAAAGACGACAATTGTAATGTAGATCTTCCTATACTCAAAGTTCTCTGTTTGGATAATATGTTGTTCAATGGTAAACCAGATATTGACAAGATTCTCTTAGGCTGCCCCATTCTAGAGGAATTGCAAACAAGTCTTGCTGCATTATATTTAAAGAAGCTTACTCCAGAAACGCCCAATTTGGCGATAACAAGTATTCCTGAAAGTCAATATATTCATGAATACTTCCTACCTAGGGTAGAGAATATGCGTTTAAAACTGGTATGAAATACGTTTTATATGTAATGTTTCGTCTATACAGAAATTGGGTATGGTTTCTAATGTTTTATCTGTGATTCATGCAGAAACTCCACATGTACTTACTAGTCCCCAATTTTGACAGTTTAATTCAATTGGAGCTATTTTTCTTTAATTTAAAAGGTAAAAGTTGGGATAAGAAGTGGAAGTGGATGCTAGCAGTGCTTCAACATAGTCCCAAGCTTCAACATTTTACCATTCATGAGGTATTACATATGTTTAGGATAATTTGATGATTTATTTTGTTAAGTCAACCTCTATTATCGATGTATCTTTGTTTTAATTCACTTGATTGTTTACAGGACATCGAAAATGGAATTTATGAGGACAATTGGAAGGACCCAGATATTGTTCCGGAATGCATTTCATCTCAGCTACGAACTTGCTTGTTTACAGGTTGCAGAGGCAGAAAGTCTGAGCTTCAATTTGCAGAGTATCTTATGAAAAATTCAAAAGTGTTACGTGCCATGACAATTCACAGTGCCTATTCCTTAAATTTAAATGCAAAGTACGAAATGTTACGGAAATTATCTGTGTGCCCAAGGGGCTGTGAACTTATATTTGATTGA
Protein sequence

>MS.gene86337.t1

MVVGREERSLFLDRSMFEKNELEGFIPTSIWTVWIGVSLQQNFGFNIVSSPSSVVTFKDYITFRHAIYSVIVSRDNTLPIRSFRLKFGIEYDINDANRFINAAIQRGIEKLDIDMFEKFNGLTSRIFSCKTLTVLKLKNLSVKDDNCNVDLPILKVLCLDNMLFNGKPDIDKILLGCPILEELQTSLAALYLKKLTPETPNLAITSIPESQYIHEYFLPRVENMRLKLKLHMYLLVPNFDSLIQLELFFFNLKGKSWDKKWKWMLAVLQHSPKLQHFTIHEDIENGIYEDNWKDPDIVPECISSQLRTCLFTGCRGRKSELQFAEYLMKNSKVLRAMTIHSAYSLNLNAKYEMLRKLSVCPRGCELIFD