AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34737


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34737.t1 MTR_8g468840 89.474 228 23 1 3 229 156 383 9.19e-131 376
MS.gene34737.t1 MTR_8g062270 65.652 230 76 1 3 229 160 389 6.20e-94 283
MS.gene34737.t1 MTR_5g092960 68.511 235 66 2 3 229 161 395 1.39e-88 269
MS.gene34737.t1 MTR_8g467800 59.130 230 87 3 7 229 157 386 7.41e-82 252
MS.gene34737.t1 MTR_5g092960 67.453 212 61 2 3 206 161 372 1.52e-72 226
MS.gene34737.t1 MTR_4g032730 56.780 236 95 3 1 234 156 386 2.07e-71 225
MS.gene34737.t1 MTR_2g083840 55.507 227 97 2 3 229 163 385 5.49e-66 211
MS.gene34737.t1 MTR_8g468810 52.340 235 95 7 3 229 166 391 1.07e-65 210
MS.gene34737.t1 MTR_8g468760 55.702 228 68 4 3 229 154 349 3.92e-65 207
MS.gene34737.t1 MTR_8g468810 53.052 213 89 5 1 206 164 372 5.00e-62 198
MS.gene34737.t1 MTR_8g058970 52.542 236 96 7 3 229 177 405 4.31e-58 191
MS.gene34737.t1 MTR_8g445780 47.210 233 112 6 3 233 148 371 8.17e-51 170
MS.gene34737.t1 MTR_8g467800 58.721 172 62 3 7 174 157 323 2.10e-49 165
MS.gene34737.t1 MTR_8g468810 56.463 147 61 2 1 147 164 307 2.17e-45 154
MS.gene34737.t1 MTR_8g468810 56.463 147 61 2 1 147 164 307 2.20e-45 154
MS.gene34737.t1 MTR_8g468810 57.241 145 59 2 3 147 166 307 4.43e-45 154
MS.gene34737.t1 MTR_8g014260 42.731 227 122 4 5 230 163 382 2.50e-44 154
MS.gene34737.t1 MTR_4g024710 42.358 229 121 7 4 229 58 278 2.69e-40 141
MS.gene34737.t1 MTR_8g014210 50.303 165 74 4 67 230 6 163 7.35e-39 134
MS.gene34737.t1 MTR_4g024745 42.174 230 122 7 4 230 12 233 2.67e-37 132
MS.gene34737.t1 MTR_4g050250 39.271 247 130 7 3 233 166 408 1.42e-32 122
MS.gene34737.t1 MTR_8g469020 35.000 240 137 5 3 229 148 381 1.27e-27 109
MS.gene34737.t1 MTR_8g058965 39.004 241 125 8 3 229 154 386 9.19e-27 107
MS.gene34737.t1 MTR_8g062310 36.019 211 114 5 19 229 169 358 3.52e-23 97.1
MS.gene34737.t1 MTR_8g062080 32.906 234 129 6 3 229 163 375 2.13e-20 89.4
MS.gene34737.t1 MTR_8g062300 32.389 247 131 7 2 229 178 407 4.08e-20 88.6
MS.gene34737.t1 MTR_8g070950 31.739 230 139 5 3 229 150 364 4.98e-20 88.2
MS.gene34737.t1 MTR_8g014160 42.038 157 83 5 3 155 186 338 3.71e-19 85.9
MS.gene34737.t1 MTR_8g062010 33.032 221 130 7 12 229 155 360 5.64e-18 82.4
MS.gene34737.t1 MTR_8g077460 45.783 83 38 3 148 229 230 306 9.40e-12 64.3
MS.gene34737.t1 MTR_5g084070 32.075 212 126 10 25 230 159 358 1.11e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34737.t1 AT4G26340 30.493 223 136 10 17 233 145 354 5.83e-11 62.0

Find 57 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGGTGAATATCTTTCTTTC+TGG 0.130738 8.1:-44888865 MS.gene34737:CDS
GGTTCAACAGATTCACTTTC+AGG 0.185864 8.1:+44888604 None:intergenic
TAAAGCAAATAACGAAATTC+TGG 0.291092 8.1:+44888388 None:intergenic
GACCTTTACTTCTGAACCTT+TGG 0.314656 8.1:-44888815 MS.gene34737:CDS
TGAACCTTTGGCCATACTTC+GGG 0.331951 8.1:-44888803 MS.gene34737:CDS
TTTGATTCTTCACCTGCATA+TGG 0.354218 8.1:-44888126 MS.gene34737:CDS
ATCAGCCATGCAGCAACTAT+TGG 0.363992 8.1:-44888673 MS.gene34737:CDS
GGAAGTTGGCACCCGAAGTA+TGG 0.375232 8.1:+44888792 None:intergenic
TTTCAATTACAAGACTCTTC+GGG 0.379284 8.1:-44888949 MS.gene34737:CDS
TTCAACCTCTTTAAGGAAGT+TGG 0.391442 8.1:+44888778 None:intergenic
GTTTCAATTACAAGACTCTT+CGG 0.396791 8.1:-44888950 MS.gene34737:CDS
CTGAACCTTTGGCCATACTT+CGG 0.415519 8.1:-44888804 MS.gene34737:CDS
CTGCCACGTTCTCGAAGATC+TGG 0.417848 8.1:-44888842 MS.gene34737:CDS
ATTATCGTTGACACTAAAGT+TGG 0.418001 8.1:-44888754 MS.gene34737:CDS
CAGCTCGAGATGTTTAATTC+CGG 0.420771 8.1:+44888555 None:intergenic
ATTCTGGAAAATCTGAAGTT+GGG 0.421868 8.1:+44888404 None:intergenic
TTATCGTTGACACTAAAGTT+GGG 0.428962 8.1:-44888753 MS.gene34737:CDS
AATTCTGGAAAATCTGAAGT+TGG 0.433100 8.1:+44888403 None:intergenic
ATTATCAGAGTTTGCAGGAT+AGG 0.455635 8.1:+44888304 None:intergenic
GTATCTCTTGGATTTGAATC+CGG 0.457351 8.1:-44888884 MS.gene34737:CDS
TGAATGTACCTCAGTCTCTC+TGG 0.459482 8.1:-44888982 MS.gene34737:CDS
ATATGCTGCAAATTTAATAA+AGG 0.461660 8.1:+44888058 None:intergenic
TGAATATTATCAGAGTTTGC+AGG 0.462707 8.1:+44888299 None:intergenic
ATTCTTCACCTGCATATGGA+TGG 0.467974 8.1:-44888122 MS.gene34737:CDS
GATCAGGCGTGTCTATTTCA+AGG 0.473258 8.1:+44888725 None:intergenic
AGACACTTGGGAACACTTTG+AGG 0.485182 8.1:+44888090 None:intergenic
ACTACTAGACATGTATCTCT+TGG 0.487664 8.1:-44888896 MS.gene34737:CDS
AGGCTAAGGTACTTGAGATC+AGG 0.500946 8.1:+44888709 None:intergenic
GTTTAATTCCGGAGAGAGCT+CGG 0.505622 8.1:+44888566 None:intergenic
AATCTCCTCCGAGCTCTCTC+CGG 0.506677 8.1:-44888574 MS.gene34737:CDS
AAGAGAGAAGAAAGAGCTCT+AGG 0.509079 8.1:+44888365 None:intergenic
GGCCAAAGGTTCAGAAGTAA+AGG 0.512790 8.1:+44888813 None:intergenic
GGGTGCCAACTTCCTTAAAG+AGG 0.520722 8.1:-44888783 MS.gene34737:CDS
TTGTAACATTGGTGAGGCTA+AGG 0.522260 8.1:+44888695 None:intergenic
ACAGATTCACTTTCAGGAGT+GGG 0.541743 8.1:+44888610 None:intergenic
AAGGTCAAATGTGAGACACT+TGG 0.550390 8.1:+44888077 None:intergenic
TTTGTCACCCATCCATATGC+AGG 0.552383 8.1:+44888114 None:intergenic
AGGTCAAATGTGAGACACTT+GGG 0.554909 8.1:+44888078 None:intergenic
TATCGTTGACACTAAAGTTG+GGG 0.556368 8.1:-44888752 MS.gene34737:CDS
GCAGCACTTTCAATGACGGC+AGG 0.556471 8.1:+44888920 None:intergenic
AACAGATTCACTTTCAGGAG+TGG 0.562125 8.1:+44888609 None:intergenic
GCAGGTGAAGAATCAAAAGA+TGG 0.562859 8.1:+44888132 None:intergenic
TTCTGGAAAATCTGAAGTTG+GGG 0.575072 8.1:+44888405 None:intergenic
TGCATGGCTGATTGTAACAT+TGG 0.576313 8.1:+44888684 None:intergenic
CCAGAAAGAAAGATATTCAC+CGG 0.581901 8.1:+44888865 None:intergenic
TTCTTCACCTGCATATGGAT+GGG 0.595592 8.1:-44888121 MS.gene34737:CDS
GCTCGGAGGAGATTGAGTAA+AGG 0.595653 8.1:+44888583 None:intergenic
AGTTGCAGCACTTTCAATGA+CGG 0.598450 8.1:+44888916 None:intergenic
TTTCCAGATCTTCGAGAACG+TGG 0.599775 8.1:+44888839 None:intergenic
GCTGTTTAGTTCAACAACAA+AGG 0.604630 8.1:-44888536 MS.gene34737:intron
GGCACCCGAAGTATGGCCAA+AGG 0.649858 8.1:+44888799 None:intergenic
AGATATATCCAGAGAGACTG+AGG 0.663217 8.1:+44888974 None:intergenic
AGTTTCCAATAGTTGCTGCA+TGG 0.666899 8.1:+44888668 None:intergenic
GGCTGATTGTAACATTGGTG+AGG 0.682471 8.1:+44888689 None:intergenic
TCTGATAATATTCAATGACA+AGG 0.686714 8.1:-44888290 MS.gene34737:intron
TATTGGAAACTTGCACAACG+TGG 0.700603 8.1:-44888656 MS.gene34737:CDS
TAATTCCGGAGAGAGCTCGG+AGG 0.748060 8.1:+44888569 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TAAAGCAAATAACGAAATTC+TGG + chr8.1:44888665-44888684 None:intergenic 25.0%
! TCTGATAATATTCAATGACA+AGG - chr8.1:44888760-44888779 MS.gene34737:CDS 25.0%
!! GATAATTTTCAACCTCTTTA+AGG + chr8.1:44888282-44888301 None:intergenic 25.0%
ATTATCGTTGACACTAAAGT+TGG - chr8.1:44888296-44888315 MS.gene34737:CDS 30.0%
GTTTCAATTACAAGACTCTT+CGG - chr8.1:44888100-44888119 MS.gene34737:CDS 30.0%
TGAATATTATCAGAGTTTGC+AGG + chr8.1:44888754-44888773 None:intergenic 30.0%
TTATCGTTGACACTAAAGTT+GGG - chr8.1:44888297-44888316 MS.gene34737:CDS 30.0%
TTTCAATTACAAGACTCTTC+GGG - chr8.1:44888101-44888120 MS.gene34737:CDS 30.0%
! AATTCTGGAAAATCTGAAGT+TGG + chr8.1:44888650-44888669 None:intergenic 30.0%
! ATTCTGGAAAATCTGAAGTT+GGG + chr8.1:44888649-44888668 None:intergenic 30.0%
AATCAAAAGATGGATCCATC+TGG + chr8.1:44888911-44888930 None:intergenic 35.0%
ACTACTAGACATGTATCTCT+TGG - chr8.1:44888154-44888173 MS.gene34737:intron 35.0%
ATTATCAGAGTTTGCAGGAT+AGG + chr8.1:44888749-44888768 None:intergenic 35.0%
CCAGAAAGAAAGATATTCAC+CGG + chr8.1:44888188-44888207 None:intergenic 35.0%
GTATCTCTTGGATTTGAATC+CGG - chr8.1:44888166-44888185 MS.gene34737:intron 35.0%
TATCGTTGACACTAAAGTTG+GGG - chr8.1:44888298-44888317 MS.gene34737:CDS 35.0%
TTTGATTCTTCACCTGCATA+TGG - chr8.1:44888924-44888943 MS.gene34737:CDS 35.0%
! GCTGTTTAGTTCAACAACAA+AGG - chr8.1:44888514-44888533 MS.gene34737:intron 35.0%
! TTCAACCTCTTTAAGGAAGT+TGG + chr8.1:44888275-44888294 None:intergenic 35.0%
! TTCTGGAAAATCTGAAGTTG+GGG + chr8.1:44888648-44888667 None:intergenic 35.0%
AACAGATTCACTTTCAGGAG+TGG + chr8.1:44888444-44888463 None:intergenic 40.0%
AAGAGAGAAGAAAGAGCTCT+AGG + chr8.1:44888688-44888707 None:intergenic 40.0%
AAGGTCAAATGTGAGACACT+TGG + chr8.1:44888976-44888995 None:intergenic 40.0%
ACAGATTCACTTTCAGGAGT+GGG + chr8.1:44888443-44888462 None:intergenic 40.0%
AGATATATCCAGAGAGACTG+AGG + chr8.1:44888079-44888098 None:intergenic 40.0%
AGGTCAAATGTGAGACACTT+GGG + chr8.1:44888975-44888994 None:intergenic 40.0%
AGTTGCAGCACTTTCAATGA+CGG + chr8.1:44888137-44888156 None:intergenic 40.0%
AGTTTCCAATAGTTGCTGCA+TGG + chr8.1:44888385-44888404 None:intergenic 40.0%
ATTCTTCACCTGCATATGGA+TGG - chr8.1:44888928-44888947 MS.gene34737:CDS 40.0%
CAGCTCGAGATGTTTAATTC+CGG + chr8.1:44888498-44888517 None:intergenic 40.0%
CCGGTGAATATCTTTCTTTC+TGG - chr8.1:44888185-44888204 MS.gene34737:intron 40.0%
GACCTTTACTTCTGAACCTT+TGG - chr8.1:44888235-44888254 MS.gene34737:intron 40.0%
GCAGGTGAAGAATCAAAAGA+TGG + chr8.1:44888921-44888940 None:intergenic 40.0%
GGTTCAACAGATTCACTTTC+AGG + chr8.1:44888449-44888468 None:intergenic 40.0%
TATTGGAAACTTGCACAACG+TGG - chr8.1:44888394-44888413 MS.gene34737:CDS 40.0%
TGCATGGCTGATTGTAACAT+TGG + chr8.1:44888369-44888388 None:intergenic 40.0%
TTCTTCACCTGCATATGGAT+GGG - chr8.1:44888929-44888948 MS.gene34737:CDS 40.0%
TTGTAACATTGGTGAGGCTA+AGG + chr8.1:44888358-44888377 None:intergenic 40.0%
! CTGTTGTCATTTACTTGCAG+TGG - chr8.1:44888612-44888631 MS.gene34737:CDS 40.0%
AGACACTTGGGAACACTTTG+AGG + chr8.1:44888963-44888982 None:intergenic 45.0%
ATCAGCCATGCAGCAACTAT+TGG - chr8.1:44888377-44888396 MS.gene34737:CDS 45.0%
ATGGATCCATCTGGCAACAA+GGG + chr8.1:44888902-44888921 None:intergenic 45.0%
CTGAACCTTTGGCCATACTT+CGG - chr8.1:44888246-44888265 MS.gene34737:intron 45.0%
GATCAGGCGTGTCTATTTCA+AGG + chr8.1:44888328-44888347 None:intergenic 45.0%
GGCCAAAGGTTCAGAAGTAA+AGG + chr8.1:44888240-44888259 None:intergenic 45.0%
GGCTGATTGTAACATTGGTG+AGG + chr8.1:44888364-44888383 None:intergenic 45.0%
GTTTAATTCCGGAGAGAGCT+CGG + chr8.1:44888487-44888506 None:intergenic 45.0%
TGAACCTTTGGCCATACTTC+GGG - chr8.1:44888247-44888266 MS.gene34737:intron 45.0%
TTTCCAGATCTTCGAGAACG+TGG + chr8.1:44888214-44888233 None:intergenic 45.0%
TTTGTCACCCATCCATATGC+AGG + chr8.1:44888939-44888958 None:intergenic 45.0%
! AGGCTAAGGTACTTGAGATC+AGG + chr8.1:44888344-44888363 None:intergenic 45.0%
GATGGATCCATCTGGCAACA+AGG + chr8.1:44888903-44888922 None:intergenic 50.0%
GCTCGGAGGAGATTGAGTAA+AGG + chr8.1:44888470-44888489 None:intergenic 50.0%
GGGTGCCAACTTCCTTAAAG+AGG - chr8.1:44888267-44888286 MS.gene34737:intron 50.0%
TGGATCCATCTGGCAACAAG+GGG + chr8.1:44888901-44888920 None:intergenic 50.0%
AATCTCCTCCGAGCTCTCTC+CGG - chr8.1:44888476-44888495 MS.gene34737:intron 55.0%
AGTTGCCCCTTGTTGCCAGA+TGG - chr8.1:44888893-44888912 MS.gene34737:CDS 55.0%
CTGCCACGTTCTCGAAGATC+TGG - chr8.1:44888208-44888227 MS.gene34737:intron 55.0%
GCAGCACTTTCAATGACGGC+AGG + chr8.1:44888133-44888152 None:intergenic 55.0%
GGAAGTTGGCACCCGAAGTA+TGG + chr8.1:44888261-44888280 None:intergenic 55.0%
TAATTCCGGAGAGAGCTCGG+AGG + chr8.1:44888484-44888503 None:intergenic 55.0%
GGCACCCGAAGTATGGCCAA+AGG + chr8.1:44888254-44888273 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 44888071 44889001 44888071 ID=MS.gene34737
chr8.1 mRNA 44888071 44889001 44888071 ID=MS.gene34737.t1;Parent=MS.gene34737
chr8.1 exon 44888537 44889001 44888537 ID=MS.gene34737.t1.exon1;Parent=MS.gene34737.t1
chr8.1 CDS 44888537 44889001 44888537 ID=cds.MS.gene34737.t1;Parent=MS.gene34737.t1
chr8.1 exon 44888291 44888440 44888291 ID=MS.gene34737.t1.exon2;Parent=MS.gene34737.t1
chr8.1 CDS 44888291 44888440 44888291 ID=cds.MS.gene34737.t1;Parent=MS.gene34737.t1
chr8.1 exon 44888071 44888160 44888071 ID=MS.gene34737.t1.exon3;Parent=MS.gene34737.t1
chr8.1 CDS 44888071 44888160 44888071 ID=cds.MS.gene34737.t1;Parent=MS.gene34737.t1
Gene Sequence

>MS.gene34737

ATGTACCTCAGTCTCTCTGGATATATCTTGTTTCAATTACAAGACTCTTCGGGTATTCACCTGCCGTCATTGAAAGTGCTGCAACTACTAGACATGTATCTCTTGGATTTGAATCCGGTGAATATCTTTCTTTCTGGCTGCCACGTTCTCGAAGATCTGGAAATGACCTTTACTTCTGAACCTTTGGCCATACTTCGGGTGCCAACTTCCTTAAAGAGGTTGAAAATTATCGTTGACACTAAAGTTGGGGCTTGCCTTGAAATAGACACGCCTGATCTCAAGTACCTTAGCCTCACCAATGTTACAATCAGCCATGCAGCAACTATTGGAAACTTGCACAACGTGGAAGAAGCATTTCTTGATGTACTTCCCACTCCTGAAAGTGAATCTGTTGAACCTTTACTCAATCTCCTCCGAGCTCTCTCCGGAATTAAACATCTCGAGCTGTTTAGTTCAACAACAAAGGTGTCGTAAGTCTCGATTATTTGTATTGTATGTAGTTTTTTCACTAGTAACTATAAAATAATCAGTTAATCATTTGCTGTTGTCATTTACTTGCAGTGGCTATCTGCTGCCCCAACTTCAGATTTTCCAGAATTTCGTTATTTGCTTTACCTAGAGCTCTTTCTTCTCTCTTTCAACTTTAATTTTATATTCGACATGCTTCATAAATGTCCTATCCTGCAAACTCTGATAATATTCAATGACAAGGTATGTTCAGTTTAACTAAAGCTTTGATGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTTGTTTGAATATTTTATGTGAGTTGCCCCTTGTTGCCAGATGGATCCATCTTTTGATTCTTCACCTGCATATGGATGGGTGACAAAGCCTCAAAGTGTTCCCAAGTGTCTCACATTTGACCTTTATTAA

Protein sequence

>MS.gene34737.t1

MYLSLSGYILFQLQDSSGIHLPSLKVLQLLDMYLLDLNPVNIFLSGCHVLEDLEMTFTSEPLAILRVPTSLKRLKIIVDTKVGACLEIDTPDLKYLSLTNVTISHAATIGNLHNVEEAFLDVLPTPESESVEPLLNLLRALSGIKHLELFSSTTKWLSAAPTSDFPEFRYLLYLELFLLSFNFNFIFDMLHKCPILQTLIIFNDKMDPSFDSSPAYGWVTKPQSVPKCLTFDLY