Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34737.t1 | KEH19814.1 | 90.9 | 232 | 20 | 1 | 3 | 233 | 156 | 387 | 6.30E-111 | 410.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34737.t1 | Q8H1R7 | 30.8 | 237 | 143 | 10 | 3 | 233 | 133 | 354 | 6.0e-09 | 62.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34737.t1 | A0A072TQN8 | 90.9 | 232 | 20 | 1 | 3 | 233 | 156 | 387 | 4.6e-111 | 410.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34737.t1 | MTR_8g468840 | 89.474 | 228 | 23 | 1 | 3 | 229 | 156 | 383 | 9.19e-131 | 376 |
| MS.gene34737.t1 | MTR_8g062270 | 65.652 | 230 | 76 | 1 | 3 | 229 | 160 | 389 | 6.20e-94 | 283 |
| MS.gene34737.t1 | MTR_5g092960 | 68.511 | 235 | 66 | 2 | 3 | 229 | 161 | 395 | 1.39e-88 | 269 |
| MS.gene34737.t1 | MTR_8g467800 | 59.130 | 230 | 87 | 3 | 7 | 229 | 157 | 386 | 7.41e-82 | 252 |
| MS.gene34737.t1 | MTR_5g092960 | 67.453 | 212 | 61 | 2 | 3 | 206 | 161 | 372 | 1.52e-72 | 226 |
| MS.gene34737.t1 | MTR_4g032730 | 56.780 | 236 | 95 | 3 | 1 | 234 | 156 | 386 | 2.07e-71 | 225 |
| MS.gene34737.t1 | MTR_2g083840 | 55.507 | 227 | 97 | 2 | 3 | 229 | 163 | 385 | 5.49e-66 | 211 |
| MS.gene34737.t1 | MTR_8g468810 | 52.340 | 235 | 95 | 7 | 3 | 229 | 166 | 391 | 1.07e-65 | 210 |
| MS.gene34737.t1 | MTR_8g468760 | 55.702 | 228 | 68 | 4 | 3 | 229 | 154 | 349 | 3.92e-65 | 207 |
| MS.gene34737.t1 | MTR_8g468810 | 53.052 | 213 | 89 | 5 | 1 | 206 | 164 | 372 | 5.00e-62 | 198 |
| MS.gene34737.t1 | MTR_8g058970 | 52.542 | 236 | 96 | 7 | 3 | 229 | 177 | 405 | 4.31e-58 | 191 |
| MS.gene34737.t1 | MTR_8g445780 | 47.210 | 233 | 112 | 6 | 3 | 233 | 148 | 371 | 8.17e-51 | 170 |
| MS.gene34737.t1 | MTR_8g467800 | 58.721 | 172 | 62 | 3 | 7 | 174 | 157 | 323 | 2.10e-49 | 165 |
| MS.gene34737.t1 | MTR_8g468810 | 56.463 | 147 | 61 | 2 | 1 | 147 | 164 | 307 | 2.17e-45 | 154 |
| MS.gene34737.t1 | MTR_8g468810 | 56.463 | 147 | 61 | 2 | 1 | 147 | 164 | 307 | 2.20e-45 | 154 |
| MS.gene34737.t1 | MTR_8g468810 | 57.241 | 145 | 59 | 2 | 3 | 147 | 166 | 307 | 4.43e-45 | 154 |
| MS.gene34737.t1 | MTR_8g014260 | 42.731 | 227 | 122 | 4 | 5 | 230 | 163 | 382 | 2.50e-44 | 154 |
| MS.gene34737.t1 | MTR_4g024710 | 42.358 | 229 | 121 | 7 | 4 | 229 | 58 | 278 | 2.69e-40 | 141 |
| MS.gene34737.t1 | MTR_8g014210 | 50.303 | 165 | 74 | 4 | 67 | 230 | 6 | 163 | 7.35e-39 | 134 |
| MS.gene34737.t1 | MTR_4g024745 | 42.174 | 230 | 122 | 7 | 4 | 230 | 12 | 233 | 2.67e-37 | 132 |
| MS.gene34737.t1 | MTR_4g050250 | 39.271 | 247 | 130 | 7 | 3 | 233 | 166 | 408 | 1.42e-32 | 122 |
| MS.gene34737.t1 | MTR_8g469020 | 35.000 | 240 | 137 | 5 | 3 | 229 | 148 | 381 | 1.27e-27 | 109 |
| MS.gene34737.t1 | MTR_8g058965 | 39.004 | 241 | 125 | 8 | 3 | 229 | 154 | 386 | 9.19e-27 | 107 |
| MS.gene34737.t1 | MTR_8g062310 | 36.019 | 211 | 114 | 5 | 19 | 229 | 169 | 358 | 3.52e-23 | 97.1 |
| MS.gene34737.t1 | MTR_8g062080 | 32.906 | 234 | 129 | 6 | 3 | 229 | 163 | 375 | 2.13e-20 | 89.4 |
| MS.gene34737.t1 | MTR_8g062300 | 32.389 | 247 | 131 | 7 | 2 | 229 | 178 | 407 | 4.08e-20 | 88.6 |
| MS.gene34737.t1 | MTR_8g070950 | 31.739 | 230 | 139 | 5 | 3 | 229 | 150 | 364 | 4.98e-20 | 88.2 |
| MS.gene34737.t1 | MTR_8g014160 | 42.038 | 157 | 83 | 5 | 3 | 155 | 186 | 338 | 3.71e-19 | 85.9 |
| MS.gene34737.t1 | MTR_8g062010 | 33.032 | 221 | 130 | 7 | 12 | 229 | 155 | 360 | 5.64e-18 | 82.4 |
| MS.gene34737.t1 | MTR_8g077460 | 45.783 | 83 | 38 | 3 | 148 | 229 | 230 | 306 | 9.40e-12 | 64.3 |
| MS.gene34737.t1 | MTR_5g084070 | 32.075 | 212 | 126 | 10 | 25 | 230 | 159 | 358 | 1.11e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34737.t1 | AT4G26340 | 30.493 | 223 | 136 | 10 | 17 | 233 | 145 | 354 | 5.83e-11 | 62.0 |
Find 57 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCGGTGAATATCTTTCTTTC+TGG | 0.130738 | 8.1:-44888865 | MS.gene34737:CDS |
| GGTTCAACAGATTCACTTTC+AGG | 0.185864 | 8.1:+44888604 | None:intergenic |
| TAAAGCAAATAACGAAATTC+TGG | 0.291092 | 8.1:+44888388 | None:intergenic |
| GACCTTTACTTCTGAACCTT+TGG | 0.314656 | 8.1:-44888815 | MS.gene34737:CDS |
| TGAACCTTTGGCCATACTTC+GGG | 0.331951 | 8.1:-44888803 | MS.gene34737:CDS |
| TTTGATTCTTCACCTGCATA+TGG | 0.354218 | 8.1:-44888126 | MS.gene34737:CDS |
| ATCAGCCATGCAGCAACTAT+TGG | 0.363992 | 8.1:-44888673 | MS.gene34737:CDS |
| GGAAGTTGGCACCCGAAGTA+TGG | 0.375232 | 8.1:+44888792 | None:intergenic |
| TTTCAATTACAAGACTCTTC+GGG | 0.379284 | 8.1:-44888949 | MS.gene34737:CDS |
| TTCAACCTCTTTAAGGAAGT+TGG | 0.391442 | 8.1:+44888778 | None:intergenic |
| GTTTCAATTACAAGACTCTT+CGG | 0.396791 | 8.1:-44888950 | MS.gene34737:CDS |
| CTGAACCTTTGGCCATACTT+CGG | 0.415519 | 8.1:-44888804 | MS.gene34737:CDS |
| CTGCCACGTTCTCGAAGATC+TGG | 0.417848 | 8.1:-44888842 | MS.gene34737:CDS |
| ATTATCGTTGACACTAAAGT+TGG | 0.418001 | 8.1:-44888754 | MS.gene34737:CDS |
| CAGCTCGAGATGTTTAATTC+CGG | 0.420771 | 8.1:+44888555 | None:intergenic |
| ATTCTGGAAAATCTGAAGTT+GGG | 0.421868 | 8.1:+44888404 | None:intergenic |
| TTATCGTTGACACTAAAGTT+GGG | 0.428962 | 8.1:-44888753 | MS.gene34737:CDS |
| AATTCTGGAAAATCTGAAGT+TGG | 0.433100 | 8.1:+44888403 | None:intergenic |
| ATTATCAGAGTTTGCAGGAT+AGG | 0.455635 | 8.1:+44888304 | None:intergenic |
| GTATCTCTTGGATTTGAATC+CGG | 0.457351 | 8.1:-44888884 | MS.gene34737:CDS |
| TGAATGTACCTCAGTCTCTC+TGG | 0.459482 | 8.1:-44888982 | MS.gene34737:CDS |
| ATATGCTGCAAATTTAATAA+AGG | 0.461660 | 8.1:+44888058 | None:intergenic |
| TGAATATTATCAGAGTTTGC+AGG | 0.462707 | 8.1:+44888299 | None:intergenic |
| ATTCTTCACCTGCATATGGA+TGG | 0.467974 | 8.1:-44888122 | MS.gene34737:CDS |
| GATCAGGCGTGTCTATTTCA+AGG | 0.473258 | 8.1:+44888725 | None:intergenic |
| AGACACTTGGGAACACTTTG+AGG | 0.485182 | 8.1:+44888090 | None:intergenic |
| ACTACTAGACATGTATCTCT+TGG | 0.487664 | 8.1:-44888896 | MS.gene34737:CDS |
| AGGCTAAGGTACTTGAGATC+AGG | 0.500946 | 8.1:+44888709 | None:intergenic |
| GTTTAATTCCGGAGAGAGCT+CGG | 0.505622 | 8.1:+44888566 | None:intergenic |
| AATCTCCTCCGAGCTCTCTC+CGG | 0.506677 | 8.1:-44888574 | MS.gene34737:CDS |
| AAGAGAGAAGAAAGAGCTCT+AGG | 0.509079 | 8.1:+44888365 | None:intergenic |
| GGCCAAAGGTTCAGAAGTAA+AGG | 0.512790 | 8.1:+44888813 | None:intergenic |
| GGGTGCCAACTTCCTTAAAG+AGG | 0.520722 | 8.1:-44888783 | MS.gene34737:CDS |
| TTGTAACATTGGTGAGGCTA+AGG | 0.522260 | 8.1:+44888695 | None:intergenic |
| ACAGATTCACTTTCAGGAGT+GGG | 0.541743 | 8.1:+44888610 | None:intergenic |
| AAGGTCAAATGTGAGACACT+TGG | 0.550390 | 8.1:+44888077 | None:intergenic |
| TTTGTCACCCATCCATATGC+AGG | 0.552383 | 8.1:+44888114 | None:intergenic |
| AGGTCAAATGTGAGACACTT+GGG | 0.554909 | 8.1:+44888078 | None:intergenic |
| TATCGTTGACACTAAAGTTG+GGG | 0.556368 | 8.1:-44888752 | MS.gene34737:CDS |
| GCAGCACTTTCAATGACGGC+AGG | 0.556471 | 8.1:+44888920 | None:intergenic |
| AACAGATTCACTTTCAGGAG+TGG | 0.562125 | 8.1:+44888609 | None:intergenic |
| GCAGGTGAAGAATCAAAAGA+TGG | 0.562859 | 8.1:+44888132 | None:intergenic |
| TTCTGGAAAATCTGAAGTTG+GGG | 0.575072 | 8.1:+44888405 | None:intergenic |
| TGCATGGCTGATTGTAACAT+TGG | 0.576313 | 8.1:+44888684 | None:intergenic |
| CCAGAAAGAAAGATATTCAC+CGG | 0.581901 | 8.1:+44888865 | None:intergenic |
| TTCTTCACCTGCATATGGAT+GGG | 0.595592 | 8.1:-44888121 | MS.gene34737:CDS |
| GCTCGGAGGAGATTGAGTAA+AGG | 0.595653 | 8.1:+44888583 | None:intergenic |
| AGTTGCAGCACTTTCAATGA+CGG | 0.598450 | 8.1:+44888916 | None:intergenic |
| TTTCCAGATCTTCGAGAACG+TGG | 0.599775 | 8.1:+44888839 | None:intergenic |
| GCTGTTTAGTTCAACAACAA+AGG | 0.604630 | 8.1:-44888536 | MS.gene34737:intron |
| GGCACCCGAAGTATGGCCAA+AGG | 0.649858 | 8.1:+44888799 | None:intergenic |
| AGATATATCCAGAGAGACTG+AGG | 0.663217 | 8.1:+44888974 | None:intergenic |
| AGTTTCCAATAGTTGCTGCA+TGG | 0.666899 | 8.1:+44888668 | None:intergenic |
| GGCTGATTGTAACATTGGTG+AGG | 0.682471 | 8.1:+44888689 | None:intergenic |
| TCTGATAATATTCAATGACA+AGG | 0.686714 | 8.1:-44888290 | MS.gene34737:intron |
| TATTGGAAACTTGCACAACG+TGG | 0.700603 | 8.1:-44888656 | MS.gene34737:CDS |
| TAATTCCGGAGAGAGCTCGG+AGG | 0.748060 | 8.1:+44888569 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAAAGCAAATAACGAAATTC+TGG | + | chr8.1:44888665-44888684 | None:intergenic | 25.0% |
| ! | TCTGATAATATTCAATGACA+AGG | - | chr8.1:44888760-44888779 | MS.gene34737:CDS | 25.0% |
| !! | GATAATTTTCAACCTCTTTA+AGG | + | chr8.1:44888282-44888301 | None:intergenic | 25.0% |
| ATTATCGTTGACACTAAAGT+TGG | - | chr8.1:44888296-44888315 | MS.gene34737:CDS | 30.0% | |
| GTTTCAATTACAAGACTCTT+CGG | - | chr8.1:44888100-44888119 | MS.gene34737:CDS | 30.0% | |
| TGAATATTATCAGAGTTTGC+AGG | + | chr8.1:44888754-44888773 | None:intergenic | 30.0% | |
| TTATCGTTGACACTAAAGTT+GGG | - | chr8.1:44888297-44888316 | MS.gene34737:CDS | 30.0% | |
| TTTCAATTACAAGACTCTTC+GGG | - | chr8.1:44888101-44888120 | MS.gene34737:CDS | 30.0% | |
| ! | AATTCTGGAAAATCTGAAGT+TGG | + | chr8.1:44888650-44888669 | None:intergenic | 30.0% |
| ! | ATTCTGGAAAATCTGAAGTT+GGG | + | chr8.1:44888649-44888668 | None:intergenic | 30.0% |
| AATCAAAAGATGGATCCATC+TGG | + | chr8.1:44888911-44888930 | None:intergenic | 35.0% | |
| ACTACTAGACATGTATCTCT+TGG | - | chr8.1:44888154-44888173 | MS.gene34737:intron | 35.0% | |
| ATTATCAGAGTTTGCAGGAT+AGG | + | chr8.1:44888749-44888768 | None:intergenic | 35.0% | |
| CCAGAAAGAAAGATATTCAC+CGG | + | chr8.1:44888188-44888207 | None:intergenic | 35.0% | |
| GTATCTCTTGGATTTGAATC+CGG | - | chr8.1:44888166-44888185 | MS.gene34737:intron | 35.0% | |
| TATCGTTGACACTAAAGTTG+GGG | - | chr8.1:44888298-44888317 | MS.gene34737:CDS | 35.0% | |
| TTTGATTCTTCACCTGCATA+TGG | - | chr8.1:44888924-44888943 | MS.gene34737:CDS | 35.0% | |
| ! | GCTGTTTAGTTCAACAACAA+AGG | - | chr8.1:44888514-44888533 | MS.gene34737:intron | 35.0% |
| ! | TTCAACCTCTTTAAGGAAGT+TGG | + | chr8.1:44888275-44888294 | None:intergenic | 35.0% |
| ! | TTCTGGAAAATCTGAAGTTG+GGG | + | chr8.1:44888648-44888667 | None:intergenic | 35.0% |
| AACAGATTCACTTTCAGGAG+TGG | + | chr8.1:44888444-44888463 | None:intergenic | 40.0% | |
| AAGAGAGAAGAAAGAGCTCT+AGG | + | chr8.1:44888688-44888707 | None:intergenic | 40.0% | |
| AAGGTCAAATGTGAGACACT+TGG | + | chr8.1:44888976-44888995 | None:intergenic | 40.0% | |
| ACAGATTCACTTTCAGGAGT+GGG | + | chr8.1:44888443-44888462 | None:intergenic | 40.0% | |
| AGATATATCCAGAGAGACTG+AGG | + | chr8.1:44888079-44888098 | None:intergenic | 40.0% | |
| AGGTCAAATGTGAGACACTT+GGG | + | chr8.1:44888975-44888994 | None:intergenic | 40.0% | |
| AGTTGCAGCACTTTCAATGA+CGG | + | chr8.1:44888137-44888156 | None:intergenic | 40.0% | |
| AGTTTCCAATAGTTGCTGCA+TGG | + | chr8.1:44888385-44888404 | None:intergenic | 40.0% | |
| ATTCTTCACCTGCATATGGA+TGG | - | chr8.1:44888928-44888947 | MS.gene34737:CDS | 40.0% | |
| CAGCTCGAGATGTTTAATTC+CGG | + | chr8.1:44888498-44888517 | None:intergenic | 40.0% | |
| CCGGTGAATATCTTTCTTTC+TGG | - | chr8.1:44888185-44888204 | MS.gene34737:intron | 40.0% | |
| GACCTTTACTTCTGAACCTT+TGG | - | chr8.1:44888235-44888254 | MS.gene34737:intron | 40.0% | |
| GCAGGTGAAGAATCAAAAGA+TGG | + | chr8.1:44888921-44888940 | None:intergenic | 40.0% | |
| GGTTCAACAGATTCACTTTC+AGG | + | chr8.1:44888449-44888468 | None:intergenic | 40.0% | |
| TATTGGAAACTTGCACAACG+TGG | - | chr8.1:44888394-44888413 | MS.gene34737:CDS | 40.0% | |
| TGCATGGCTGATTGTAACAT+TGG | + | chr8.1:44888369-44888388 | None:intergenic | 40.0% | |
| TTCTTCACCTGCATATGGAT+GGG | - | chr8.1:44888929-44888948 | MS.gene34737:CDS | 40.0% | |
| TTGTAACATTGGTGAGGCTA+AGG | + | chr8.1:44888358-44888377 | None:intergenic | 40.0% | |
| ! | CTGTTGTCATTTACTTGCAG+TGG | - | chr8.1:44888612-44888631 | MS.gene34737:CDS | 40.0% |
| AGACACTTGGGAACACTTTG+AGG | + | chr8.1:44888963-44888982 | None:intergenic | 45.0% | |
| ATCAGCCATGCAGCAACTAT+TGG | - | chr8.1:44888377-44888396 | MS.gene34737:CDS | 45.0% | |
| ATGGATCCATCTGGCAACAA+GGG | + | chr8.1:44888902-44888921 | None:intergenic | 45.0% | |
| CTGAACCTTTGGCCATACTT+CGG | - | chr8.1:44888246-44888265 | MS.gene34737:intron | 45.0% | |
| GATCAGGCGTGTCTATTTCA+AGG | + | chr8.1:44888328-44888347 | None:intergenic | 45.0% | |
| GGCCAAAGGTTCAGAAGTAA+AGG | + | chr8.1:44888240-44888259 | None:intergenic | 45.0% | |
| GGCTGATTGTAACATTGGTG+AGG | + | chr8.1:44888364-44888383 | None:intergenic | 45.0% | |
| GTTTAATTCCGGAGAGAGCT+CGG | + | chr8.1:44888487-44888506 | None:intergenic | 45.0% | |
| TGAACCTTTGGCCATACTTC+GGG | - | chr8.1:44888247-44888266 | MS.gene34737:intron | 45.0% | |
| TTTCCAGATCTTCGAGAACG+TGG | + | chr8.1:44888214-44888233 | None:intergenic | 45.0% | |
| TTTGTCACCCATCCATATGC+AGG | + | chr8.1:44888939-44888958 | None:intergenic | 45.0% | |
| ! | AGGCTAAGGTACTTGAGATC+AGG | + | chr8.1:44888344-44888363 | None:intergenic | 45.0% |
| GATGGATCCATCTGGCAACA+AGG | + | chr8.1:44888903-44888922 | None:intergenic | 50.0% | |
| GCTCGGAGGAGATTGAGTAA+AGG | + | chr8.1:44888470-44888489 | None:intergenic | 50.0% | |
| GGGTGCCAACTTCCTTAAAG+AGG | - | chr8.1:44888267-44888286 | MS.gene34737:intron | 50.0% | |
| TGGATCCATCTGGCAACAAG+GGG | + | chr8.1:44888901-44888920 | None:intergenic | 50.0% | |
| AATCTCCTCCGAGCTCTCTC+CGG | - | chr8.1:44888476-44888495 | MS.gene34737:intron | 55.0% | |
| AGTTGCCCCTTGTTGCCAGA+TGG | - | chr8.1:44888893-44888912 | MS.gene34737:CDS | 55.0% | |
| CTGCCACGTTCTCGAAGATC+TGG | - | chr8.1:44888208-44888227 | MS.gene34737:intron | 55.0% | |
| GCAGCACTTTCAATGACGGC+AGG | + | chr8.1:44888133-44888152 | None:intergenic | 55.0% | |
| GGAAGTTGGCACCCGAAGTA+TGG | + | chr8.1:44888261-44888280 | None:intergenic | 55.0% | |
| TAATTCCGGAGAGAGCTCGG+AGG | + | chr8.1:44888484-44888503 | None:intergenic | 55.0% | |
| GGCACCCGAAGTATGGCCAA+AGG | + | chr8.1:44888254-44888273 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 44888071 | 44889001 | 44888071 | ID=MS.gene34737 |
| chr8.1 | mRNA | 44888071 | 44889001 | 44888071 | ID=MS.gene34737.t1;Parent=MS.gene34737 |
| chr8.1 | exon | 44888537 | 44889001 | 44888537 | ID=MS.gene34737.t1.exon1;Parent=MS.gene34737.t1 |
| chr8.1 | CDS | 44888537 | 44889001 | 44888537 | ID=cds.MS.gene34737.t1;Parent=MS.gene34737.t1 |
| chr8.1 | exon | 44888291 | 44888440 | 44888291 | ID=MS.gene34737.t1.exon2;Parent=MS.gene34737.t1 |
| chr8.1 | CDS | 44888291 | 44888440 | 44888291 | ID=cds.MS.gene34737.t1;Parent=MS.gene34737.t1 |
| chr8.1 | exon | 44888071 | 44888160 | 44888071 | ID=MS.gene34737.t1.exon3;Parent=MS.gene34737.t1 |
| chr8.1 | CDS | 44888071 | 44888160 | 44888071 | ID=cds.MS.gene34737.t1;Parent=MS.gene34737.t1 |
| Gene Sequence |
| Protein sequence |