AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34215

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34215.t1 MTR_8g058970 94.919 433 20 1 1 431 45 477 0.0 823
MS.gene34215.t1 MTR_8g468810 64.815 432 132 4 1 431 51 463 0.0 538
MS.gene34215.t1 MTR_2g083840 54.734 433 168 8 3 431 49 457 6.00e-149 431
MS.gene34215.t1 MTR_8g468810 66.176 340 95 5 1 339 51 371 1.05e-147 425
MS.gene34215.t1 MTR_4g032730 48.908 458 170 15 3 430 43 466 2.08e-125 371
MS.gene34215.t1 MTR_8g468810 63.763 287 85 3 1 287 51 318 5.18e-119 350
MS.gene34215.t1 MTR_8g468810 64.437 284 82 3 1 284 51 315 7.66e-119 350
MS.gene34215.t1 MTR_8g468810 64.437 284 82 3 1 284 51 315 2.20e-118 349
MS.gene34215.t1 MTR_8g062270 47.865 445 185 12 1 430 41 453 1.66e-116 348
MS.gene34215.t1 MTR_5g092960 49.103 446 178 14 3 430 47 461 2.10e-115 346
MS.gene34215.t1 MTR_8g467800 48.874 444 177 14 2 431 46 453 7.19e-115 344
MS.gene34215.t1 MTR_8g468840 46.469 439 192 12 2 428 40 447 1.82e-109 330
MS.gene34215.t1 MTR_8g468760 45.270 444 170 12 1 430 34 418 9.28e-100 304
MS.gene34215.t1 MTR_8g014260 42.890 436 213 15 4 431 44 451 1.33e-97 300
MS.gene34215.t1 MTR_8g469020 40.177 453 213 18 2 431 34 451 6.81e-86 270
MS.gene34215.t1 MTR_8g445780 44.909 383 172 12 4 381 41 389 2.64e-85 266
MS.gene34215.t1 MTR_5g092960 47.714 350 145 11 3 339 47 371 3.32e-79 251
MS.gene34215.t1 MTR_8g058965 38.616 448 226 14 2 431 40 456 1.36e-77 248
MS.gene34215.t1 MTR_8g070950 35.794 447 218 14 1 431 41 434 1.60e-69 227
MS.gene34215.t1 MTR_4g050250 39.747 395 193 9 1 373 47 418 2.41e-69 226
MS.gene34215.t1 MTR_8g062010 37.300 437 227 14 1 431 35 430 5.63e-68 223
MS.gene34215.t1 MTR_8g062080 36.323 446 220 15 1 431 49 445 4.64e-66 218
MS.gene34215.t1 MTR_8g467800 49.653 288 116 8 2 284 46 309 1.87e-65 213
MS.gene34215.t1 MTR_8g014160 35.160 438 197 11 2 431 67 425 3.32e-65 215
MS.gene34215.t1 MTR_4g024710 44.371 302 152 10 134 431 58 347 8.01e-65 212
MS.gene34215.t1 MTR_8g062300 35.652 460 233 13 1 431 52 477 1.28e-64 215
MS.gene34215.t1 MTR_8g062310 35.963 431 217 14 4 431 54 428 1.32e-62 208
MS.gene34215.t1 MTR_4g024745 42.857 280 149 8 132 408 10 281 3.15e-52 177
MS.gene34215.t1 MTR_8g014210 44.215 242 123 6 191 431 1 231 4.27e-50 169
MS.gene34215.t1 MTR_8g077460 31.279 438 191 18 4 431 38 375 4.64e-45 160
MS.gene34215.t1 MTR_8g467790 32.216 388 186 14 1 376 174 496 1.13e-38 146
MS.gene34215.t1 MTR_5g084070 28.738 428 252 16 2 423 39 419 2.17e-28 116
MS.gene34215.t1 MTR_5g080710 26.897 435 258 15 2 423 59 446 1.33e-23 102
MS.gene34215.t1 MTR_2g086020 45.522 134 68 3 300 431 185 315 3.67e-22 97.1
MS.gene34215.t1 MTR_2g086020 31.858 226 111 8 1 224 1 185 6.21e-17 81.6
MS.gene34215.t1 MTR_4g056040 28.040 403 229 14 41 419 99 464 5.08e-21 95.5
MS.gene34215.t1 MTR_5g021550 38.636 132 63 4 4 133 47 162 7.79e-20 86.7
MS.gene34215.t1 MTR_4g056070 27.624 362 203 13 62 381 92 436 5.02e-19 89.4
MS.gene34215.t1 MTR_3g087260 29.752 363 183 13 120 430 114 456 4.52e-18 86.7
MS.gene34215.t1 MTR_8g014290 37.410 139 63 6 4 134 38 160 9.32e-18 80.5
MS.gene34215.t1 MTR_3g108150 25.682 440 224 19 2 430 39 386 9.14e-17 82.0
MS.gene34215.t1 MTR_4g055990 26.329 414 250 16 48 419 76 476 1.12e-16 82.4
MS.gene34215.t1 MTR_4g055990 26.329 414 250 16 48 419 76 476 1.24e-16 82.4
MS.gene34215.t1 MTR_7g098510 35.461 141 80 4 298 431 255 391 2.58e-16 80.5
MS.gene34215.t1 MTR_3g036250 28.870 239 143 6 203 428 139 363 4.18e-16 79.7
MS.gene34215.t1 MTR_7g056433 35.417 144 77 6 297 431 253 389 8.17e-16 79.3
MS.gene34215.t1 MTR_4g056020 26.667 390 222 15 41 406 69 418 1.12e-15 79.3
MS.gene34215.t1 MTR_1g041245 34.667 150 83 5 293 431 241 386 1.04e-14 75.9
MS.gene34215.t1 MTR_1089s0010 26.908 249 154 8 200 431 142 379 1.11e-14 75.5
MS.gene34215.t1 MTR_5g058100 27.106 273 174 8 179 430 115 383 5.13e-14 73.6
MS.gene34215.t1 MTR_4g056030 26.525 377 222 14 41 396 69 411 5.84e-14 73.9
MS.gene34215.t1 MTR_7g098570 29.304 273 150 12 181 427 134 389 6.46e-14 73.9
MS.gene34215.t1 MTR_7g098480 24.374 439 228 17 2 430 44 388 1.75e-13 72.4
MS.gene34215.t1 MTR_6g040270 23.799 437 231 20 3 429 35 379 2.60e-13 71.6
MS.gene34215.t1 MTR_7g026500 23.588 301 195 8 149 430 110 394 2.61e-13 71.6
MS.gene34215.t1 MTR_3g108560 25.227 440 221 19 2 430 46 388 3.05e-13 71.2
MS.gene34215.t1 MTR_8g014230 46.377 69 36 1 347 415 25 92 9.70e-13 64.7
MS.gene34215.t1 MTR_2g020180 35.211 142 69 5 298 431 89 215 1.19e-12 67.4
MS.gene34215.t1 MTR_4g113810 34.211 152 94 4 85 232 13 162 1.41e-12 65.9
MS.gene34215.t1 MTR_1g108800 29.181 281 161 11 174 429 73 340 1.58e-12 68.9
MS.gene34215.t1 MTR_0543s0010 34.752 141 71 6 303 430 317 449 1.61e-12 69.3
MS.gene34215.t1 MTR_4g055580 25.648 386 211 15 42 396 198 538 1.73e-12 69.7
MS.gene34215.t1 MTR_7g032370 32.374 139 83 3 301 431 217 352 2.28e-12 68.6
MS.gene34215.t1 MTR_1g085120 25.926 378 197 16 66 431 165 471 2.39e-12 68.9
MS.gene34215.t1 MTR_7g033915 34.091 132 79 3 303 430 237 364 3.39e-12 67.8
MS.gene34215.t1 MTR_1g085120 25.397 378 199 15 66 431 240 546 3.56e-12 68.6
MS.gene34215.t1 MTR_5g013500 30.000 140 91 3 297 431 228 365 3.62e-12 67.8
MS.gene34215.t1 MTR_7g032380 31.618 136 83 3 303 431 224 356 7.57e-12 66.6
MS.gene34215.t1 MTR_5g008950 28.085 235 140 10 217 431 140 365 1.03e-11 66.6
MS.gene34215.t1 MTR_3g040700 27.232 224 137 6 199 408 171 382 1.04e-11 67.0
MS.gene34215.t1 MTR_3g105060 33.588 131 75 3 301 431 255 373 1.32e-11 66.2
MS.gene34215.t1 MTR_7g034120 30.827 133 80 3 303 431 184 308 1.78e-11 65.5
MS.gene34215.t1 MTR_5g058000 25.468 267 156 8 187 430 128 374 1.83e-11 65.9
MS.gene34215.t1 MTR_4g080773 24.402 418 265 18 2 396 45 434 2.85e-11 65.5
MS.gene34215.t1 MTR_4g080773 24.402 418 265 18 2 396 45 434 2.87e-11 65.5
MS.gene34215.t1 MTR_7g114710 31.034 145 86 3 296 431 225 364 3.41e-11 64.7
MS.gene34215.t1 MTR_1g038710 24.638 414 260 14 3 396 46 427 3.53e-11 65.1
MS.gene34215.t1 MTR_5g012840 34.815 135 73 4 304 431 192 318 6.88e-11 63.5
MS.gene34215.t1 MTR_6g022500 25.507 345 186 11 92 431 198 476 9.82e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34215.t1 AT1G16930 26.013 469 248 22 2 431 41 449 4.31e-25 107
MS.gene34215.t1 AT1G16930 26.013 469 248 22 2 431 41 449 4.31e-25 107
MS.gene34215.t1 AT1G16930 26.013 469 248 22 2 431 41 449 4.31e-25 107
MS.gene34215.t1 AT4G26340 25.339 442 268 16 2 431 28 419 1.43e-23 102
MS.gene34215.t1 AT5G56420 25.909 440 267 15 2 431 32 422 9.54e-23 100
MS.gene34215.t1 AT5G56420 25.909 440 267 15 2 431 32 422 9.54e-23 100
MS.gene34215.t1 AT5G56420 25.909 440 267 15 2 431 32 422 9.54e-23 100
MS.gene34215.t1 AT5G62970 27.273 407 252 13 32 430 78 448 1.32e-21 97.1
MS.gene34215.t1 AT1G78750 25.390 449 276 16 2 428 44 455 1.80e-21 96.7
MS.gene34215.t1 AT1G78750 25.390 449 276 16 2 428 44 455 1.80e-21 96.7
MS.gene34215.t1 AT5G22720 26.004 473 249 23 2 430 49 464 1.17e-19 91.3
MS.gene34215.t1 AT3G54910 25.564 399 229 16 58 431 18 373 2.02e-18 87.0
MS.gene34215.t1 AT3G54910 25.564 399 229 16 58 431 18 373 2.02e-18 87.0
MS.gene34215.t1 AT3G54910 25.564 399 229 16 58 431 18 373 2.02e-18 87.0
MS.gene34215.t1 AT1G51370 26.622 447 245 23 4 428 47 432 4.44e-18 86.3
MS.gene34215.t1 AT4G13985 24.236 458 272 17 1 428 44 456 8.10e-18 85.9
MS.gene34215.t1 AT5G56390 25.167 449 261 18 3 430 30 424 9.24e-18 85.5
MS.gene34215.t1 AT5G22730 27.493 371 213 14 91 429 118 464 1.86e-17 84.7
MS.gene34215.t1 AT5G22730 27.493 371 213 14 91 429 118 464 1.86e-17 84.7
MS.gene34215.t1 AT1G13780 24.454 458 274 19 2 428 36 452 1.94e-17 84.7
MS.gene34215.t1 AT1G69630 24.236 458 263 20 2 428 44 448 3.10e-17 84.0
MS.gene34215.t1 AT5G38570 29.091 275 162 9 164 430 126 375 8.48e-17 82.0
MS.gene34215.t1 AT1G32375 26.124 356 224 13 91 431 91 422 1.17e-16 82.0
MS.gene34215.t1 AT1G32375 26.124 356 224 13 91 431 91 422 1.17e-16 82.0
MS.gene34215.t1 AT5G56370 24.494 445 268 18 2 430 28 420 1.27e-16 82.0
MS.gene34215.t1 AT5G56370 24.494 445 268 18 2 430 28 420 1.27e-16 82.0
MS.gene34215.t1 AT4G00160 26.269 453 257 19 2 430 42 441 1.48e-16 82.0
MS.gene34215.t1 AT5G56700 27.302 315 192 13 93 392 54 346 9.46e-16 78.6
MS.gene34215.t1 AT3G50710 28.809 361 212 17 91 431 92 427 1.12e-15 79.0
MS.gene34215.t1 AT3G26930 25.272 459 254 19 1 431 1 398 1.85e-15 78.2
MS.gene34215.t1 AT3G26930 25.272 459 254 19 1 431 46 443 1.94e-15 78.6
MS.gene34215.t1 AT4G10400 23.462 439 268 17 2 430 28 408 2.12e-15 78.2
MS.gene34215.t1 AT4G10400 23.462 439 268 17 2 430 28 408 2.12e-15 78.2
MS.gene34215.t1 AT4G10400 23.462 439 268 17 2 430 28 408 2.12e-15 78.2
MS.gene34215.t1 AT5G27750 27.397 365 196 17 101 431 128 457 2.20e-15 78.2
MS.gene34215.t1 AT5G44950 28.571 301 181 13 148 428 149 435 2.23e-15 78.2
MS.gene34215.t1 AT1G80470 22.748 444 272 21 4 428 43 434 2.47e-15 78.2
MS.gene34215.t1 AT4G26350 25.658 456 258 18 2 431 28 428 2.84e-15 77.8
MS.gene34215.t1 AT5G56820 28.242 347 196 17 101 428 111 423 9.61e-15 76.3
MS.gene34215.t1 AT5G53635 23.714 447 274 17 3 430 27 425 2.62e-14 74.7
MS.gene34215.t1 AT5G44980 29.206 315 176 17 141 430 141 433 5.57e-14 73.9
MS.gene34215.t1 AT5G44980 29.206 315 176 17 141 430 141 433 5.57e-14 73.9
MS.gene34215.t1 AT3G26920 27.247 356 219 16 89 430 10 339 1.10e-13 72.4
MS.gene34215.t1 AT3G26920 27.247 356 219 16 89 430 10 339 1.10e-13 72.4
MS.gene34215.t1 AT5G50270 24.590 427 251 18 3 408 30 406 1.14e-13 72.8
MS.gene34215.t1 AT5G44970 27.628 333 198 15 83 394 40 350 2.76e-13 71.6
MS.gene34215.t1 AT3G49030 24.612 451 261 18 2 430 47 440 4.47e-13 71.2
MS.gene34215.t1 AT4G00315 26.269 453 270 21 2 430 28 440 4.52e-13 71.2
MS.gene34215.t1 AT4G13965 27.027 444 248 20 2 428 36 420 5.42e-13 70.9
MS.gene34215.t1 AT5G56560 23.810 441 269 16 3 431 33 418 5.62e-13 70.9
MS.gene34215.t1 AT5G22660 26.400 375 222 18 86 428 91 443 9.34e-13 70.1
MS.gene34215.t1 AT5G56800 26.846 298 174 11 149 430 74 343 1.07e-12 69.3
MS.gene34215.t1 AT2G26860 29.110 292 175 11 120 397 124 397 1.60e-12 69.3
MS.gene34215.t1 AT2G26860 29.110 292 175 11 120 397 124 397 1.60e-12 69.3
MS.gene34215.t1 AT5G22670 26.039 361 224 16 94 431 102 442 2.34e-12 68.9
MS.gene34215.t1 AT3G49030 24.318 440 250 16 2 430 47 414 2.48e-12 68.6
MS.gene34215.t1 AT5G56400 24.289 457 254 18 4 431 62 455 5.07e-12 67.8
MS.gene34215.t1 AT4G10420 24.148 352 220 13 94 430 2 321 8.08e-12 66.6
MS.gene34215.t1 AT5G60610 24.629 337 220 12 75 397 71 387 8.61e-12 67.0
MS.gene34215.t1 AT5G60610 24.629 337 220 12 75 397 71 387 8.61e-12 67.0
MS.gene34215.t1 AT5G56325 26.667 285 155 13 170 431 11 264 1.01e-11 65.5
MS.gene34215.t1 AT1G55030 30.137 292 170 12 118 397 131 400 1.27e-11 66.6
MS.gene34215.t1 AT1G55030 30.137 292 170 12 118 397 121 390 1.61e-11 66.2
MS.gene34215.t1 AT1G55660 26.316 361 224 15 94 430 149 491 3.46e-11 65.5

Find 101 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAAGAAAATGATTATTTC+TGG 0.183524 8.4:-48075047 MS.gene34215:CDS
TTGTCCAATATGCACATTAA+AGG 0.261211 8.4:-48075131 MS.gene34215:CDS
ACTTCTCCCACTTCTTCTTT+TGG 0.291320 8.4:+48075012 None:intergenic
ATCATTGGCAAATGTAATTC+TGG 0.307551 8.4:+48075713 None:intergenic
ATACCCTTTAATGTGCATAT+TGG 0.316620 8.4:+48075127 None:intergenic
ACGAAGCTTGGCCAGAGATA+TGG 0.321507 8.4:+48075833 None:intergenic
AGACAGTTAGGAACAGTATT+AGG 0.329586 8.4:+48075164 None:intergenic
CAAGTAAGCATGTGGGTCTT+CGG 0.352555 8.4:+48077463 None:intergenic
AGTTCCAGGGTTTCTAAGAT+TGG 0.354019 8.4:+48075867 None:intergenic
TTCAAACTCTTCAAGGTAGT+TGG 0.363503 8.4:+48075807 None:intergenic
TGTAGTAAATGGTGAAATTC+CGG 0.365973 8.4:+48075401 None:intergenic
GTTTAATTCCAGTGAGGGCT+TGG 0.368316 8.4:+48075595 None:intergenic
TGGGAGAAAAGGCAAGTTCC+AGG 0.381998 8.4:+48075853 None:intergenic
TGAGCTCTAGATGTAGTAAA+TGG 0.396405 8.4:+48075390 None:intergenic
ACCGCCAAGCCACTTTCATT+TGG 0.398775 8.4:+48075191 None:intergenic
CGACAGACATTTCAGTAAAC+CGG 0.402724 8.4:+48077414 None:intergenic
CGTAGTAGAAGTTGAATGAT+TGG 0.408876 8.4:+48077621 None:intergenic
CGAAGCTTGGCCAGAGATAT+GGG 0.415687 8.4:+48075834 None:intergenic
GTCCCCAACCGCAGTATCAT+TGG 0.423942 8.4:+48075698 None:intergenic
CGTGGGTCCGATCCGGTGCC+CGG 0.424227 8.4:-48077433 MS.gene34215:CDS
GGTGAGTTTCAAACTCTTCA+AGG 0.426441 8.4:+48075800 None:intergenic
AAGTAAGCATGTGGGTCTTC+GGG 0.436541 8.4:+48077464 None:intergenic
TTCGGAGTTGGCGCCGAGGA+TGG 0.443651 8.4:+48077589 None:intergenic
TTGCCAATGATACTGCGGTT+GGG 0.445868 8.4:-48075701 MS.gene34215:CDS
TGTCCAATATGCACATTAAA+GGG 0.450045 8.4:-48075130 MS.gene34215:CDS
GGCCAGAGATATGGGAGAAA+AGG 0.451259 8.4:+48075842 None:intergenic
TGCCAATGATACTGCGGTTG+GGG 0.453821 8.4:-48075700 MS.gene34215:CDS
AAGTGAGACACAAGACAGTT+AGG 0.455291 8.4:+48075152 None:intergenic
TACCTGATGGAGAGTAGCTT+AGG 0.460330 8.4:+48077284 None:intergenic
GTTCCAGGGTTTCTAAGATT+GGG 0.463116 8.4:+48075868 None:intergenic
CTGAAATGTCTGTCGATGCT+TGG 0.465339 8.4:-48077406 MS.gene34215:CDS
ATTCTCCTCCAAGCCCTCAC+TGG 0.466739 8.4:-48075603 MS.gene34215:CDS
CACGCAGATATCGTCATCTC+TGG 0.468968 8.4:-48077652 MS.gene34215:CDS
AGTTTCAGCATCTTTAATGA+AGG 0.469909 8.4:+48075942 None:intergenic
TTACGCTCCTGAAAATGAAT+CGG 0.471616 8.4:-48075640 MS.gene34215:CDS
TCGGAGTTGGCGCCGAGGAT+GGG 0.473211 8.4:+48077590 None:intergenic
TCCGTGGATATCCTTCTCTC+TGG 0.481451 8.4:-48075894 MS.gene34215:CDS
ATGTAATTCTGGTGAGGCTA+AGG 0.489727 8.4:+48075724 None:intergenic
GAAGCGGACCAGCGGCAATA+CGG 0.496795 8.4:+48077381 None:intergenic
AACGACATTCCATCGACGTT+CGG 0.497996 8.4:-48077523 MS.gene34215:CDS
GCCCTAAGCTACTCTCCATC+AGG 0.499571 8.4:-48077286 MS.gene34215:intron
ATGCACATTAAAGGGTATCA+AGG 0.500704 8.4:-48075122 MS.gene34215:CDS
AGGCTAAGGTATTTGAGATC+GGG 0.502253 8.4:+48075738 None:intergenic
ACGCATCTTCCGAACGTCGA+TGG 0.503115 8.4:+48077514 None:intergenic
GCTTGGATCCGTATTGCCGC+TGG 0.505305 8.4:-48077389 MS.gene34215:CDS
TTTGCCAATGATACTGCGGT+TGG 0.505561 8.4:-48075702 MS.gene34215:CDS
CTTGGAGGAGAATGAGTAAA+GGG 0.505890 8.4:+48075613 None:intergenic
TGCCGCTGGTCCGCTTCTTG+AGG 0.510807 8.4:-48077375 MS.gene34215:CDS
TATGACTCGTGGGTCCGATC+CGG 0.513182 8.4:-48077440 MS.gene34215:CDS
ATTATAAGAGCTTGAAGCAT+AGG 0.517782 8.4:+48075320 None:intergenic
ACCAAATGAAAGTGGCTTGG+CGG 0.520565 8.4:-48075192 MS.gene34215:CDS
GGTAGTTGGAACACGAAGCT+TGG 0.523289 8.4:+48075821 None:intergenic
GAGGCTAAGGTATTTGAGAT+CGG 0.524945 8.4:+48075737 None:intergenic
ATGAGAGAACAGTGAGAGGA+AGG 0.526517 8.4:+48077309 None:intergenic
CTTGGACCAAAAGAAGAAGT+GGG 0.532722 8.4:-48075018 MS.gene34215:CDS
GACGATATCTGCGTGAGACG+AGG 0.533696 8.4:+48077660 None:intergenic
TCTTGGACCAAAAGAAGAAG+TGG 0.534181 8.4:-48075019 MS.gene34215:CDS
GAGCACAGCGTTGACGAAAA+CGG 0.534757 8.4:+48077550 None:intergenic
TAATTCCAGTGAGGGCTTGG+AGG 0.538544 8.4:+48075598 None:intergenic
TCAAGGAGATTCTAGTGAGA+TGG 0.541910 8.4:-48075105 MS.gene34215:CDS
ACCTGATGGAGAGTAGCTTA+GGG 0.542326 8.4:+48077285 None:intergenic
TAGCTCAGTCAAATGTAACT+TGG 0.544825 8.4:+48074940 None:intergenic
TGCTTACTTGTATGACTCGT+GGG 0.545376 8.4:-48077450 MS.gene34215:CDS
ATCACCAAATGAAAGTGGCT+TGG 0.546941 8.4:-48075195 MS.gene34215:CDS
TAGACGTTTAATTCCAGTGA+GGG 0.556658 8.4:+48075590 None:intergenic
ATGCTTACTTGTATGACTCG+TGG 0.559289 8.4:-48077451 MS.gene34215:CDS
CGGAAGATGCGTCTCTCATG+TGG 0.561584 8.4:-48077503 MS.gene34215:CDS
CAGTAAACCGGGCACCGGAT+CGG 0.564977 8.4:+48077426 None:intergenic
CTGCCCAATCTTAGAAACCC+TGG 0.571246 8.4:-48075871 MS.gene34215:CDS
TGTAAGAAGAGACTGGTAGA+TGG 0.572092 8.4:-48075458 MS.gene34215:intron
ACGATATCTGCGTGAGACGA+GGG 0.579271 8.4:+48077661 None:intergenic
AGATTCATTTCCTCAAGAAG+CGG 0.582908 8.4:+48077365 None:intergenic
CATTTCAGTAAACCGGGCAC+CGG 0.585179 8.4:+48077421 None:intergenic
TGGCAGACAGCAGAGCCCCT+CGG 0.585541 8.4:+48074960 None:intergenic
GATACATCACCAAATGAAAG+TGG 0.589613 8.4:-48075200 MS.gene34215:CDS
CTCACCGTTGAGAATGACAT+TGG 0.590348 8.4:-48075783 MS.gene34215:CDS
GGGAGAAAAGGCAAGTTCCA+GGG 0.590931 8.4:+48075854 None:intergenic
GCTTGGAGGAGAATGAGTAA+AGG 0.594619 8.4:+48075612 None:intergenic
TACGACTATAATCCCATCCT+CGG 0.598194 8.4:-48077602 MS.gene34215:CDS
AGCTCCAATGTCATTCTCAA+CGG 0.598655 8.4:+48075779 None:intergenic
TTCCTCAAGAAGCGGACCAG+CGG 0.605098 8.4:+48077373 None:intergenic
GTACAGAAAAGTGTCGAAAG+TGG 0.605540 8.4:+48075361 None:intergenic
GAAACTAGACATTGGTAATG+TGG 0.606340 8.4:-48075925 MS.gene34215:CDS
TACATTTGCCAATGATACTG+CGG 0.609576 8.4:-48075706 MS.gene34215:CDS
AGATTGGGCAGCCAGAGAGA+AGG 0.610124 8.4:+48075883 None:intergenic
GAGTCATACAAGTAAGCATG+TGG 0.610775 8.4:+48077455 None:intergenic
TACAGAAAAGTGTCGAAAGT+GGG 0.617739 8.4:+48075362 None:intergenic
GACAGACATTTCAGTAAACC+GGG 0.617976 8.4:+48077415 None:intergenic
GGGCATGAGAGAACAGTGAG+AGG 0.621139 8.4:+48077305 None:intergenic
ATTGCGATGTTATACAGCAA+AGG 0.632025 8.4:-48075565 MS.gene34215:intron
AAGATGCTGAAACTAGACAT+TGG 0.644934 8.4:-48075933 MS.gene34215:CDS
TAGAAAATTTGCTAACACAA+CGG 0.648999 8.4:+48074988 None:intergenic
TGGCAAATGTAATTCTGGTG+AGG 0.653371 8.4:+48075718 None:intergenic
AGAGAACAGTGAGAGGAAGG+CGG 0.655232 8.4:+48077312 None:intergenic
TCTCTGCGGTGATATCGTTG+TGG 0.661941 8.4:-48075994 MS.gene34215:CDS
GCCAGAGAGAAGGATATCCA+CGG 0.665335 8.4:+48075893 None:intergenic
TCTTATAATTCAGAACGACA+AGG 0.672226 8.4:-48075306 MS.gene34215:intron
TAATGTGGATGTGAATTCCG+TGG 0.693852 8.4:-48075910 MS.gene34215:CDS
AGTCATACAAGTAAGCATGT+GGG 0.710464 8.4:+48077456 None:intergenic
CTAGACGTTTAATTCCAGTG+AGG 0.740201 8.4:+48075589 None:intergenic
TGTAATGTACTCAGTCTCTG+CGG 0.744171 8.4:-48076008 MS.gene34215:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTCGAAAAATTTATAAT+AGG + chr8.4:48077181-48077200 None:intergenic 10.0%
!! ATTACATAAATGTTATTATA+AGG + chr8.4:48076587-48076606 None:intergenic 10.0%
!! TTTAAATGAATTGTATAATT+GGG - chr8.4:48076254-48076273 MS.gene34215:intron 10.0%
!! ACATGTTAAGAAATTTAAAA+TGG - chr8.4:48075596-48075615 MS.gene34215:CDS 15.0%
!! GTTTAAATGAATTGTATAAT+TGG - chr8.4:48076253-48076272 MS.gene34215:intron 15.0%
!! TATATTTAATATGAAATGCA+TGG + chr8.4:48076066-48076085 None:intergenic 15.0%
!! TTAAAGAAAATGATTATTTC+TGG - chr8.4:48077562-48077581 MS.gene34215:CDS 15.0%
!! AAAATTAAGAAAATACCTGA+TGG + chr8.4:48075341-48075360 None:intergenic 20.0%
!! ACTTAAATAACTTAAACTAC+AGG - chr8.4:48075758-48075777 MS.gene34215:CDS 20.0%
!! ATCCATAACAATATTTAAAG+TGG + chr8.4:48076310-48076329 None:intergenic 20.0%
!! TATAGATATACTTAGATGAT+GGG - chr8.4:48076330-48076349 MS.gene34215:intron 20.0%
!! TTATAGATATACTTAGATGA+TGG - chr8.4:48076329-48076348 MS.gene34215:intron 20.0%
!! TTCCACTTTAAATATTGTTA+TGG - chr8.4:48076305-48076324 MS.gene34215:intron 20.0%
!!! AAATGCATGGTTTTAAAAAA+TGG + chr8.4:48076053-48076072 None:intergenic 20.0%
!!! AACTTAGTGTTAACATATTT+TGG + chr8.4:48075402-48075421 None:intergenic 20.0%
!!! ACAATATTTAAAGTGGAAAA+CGG + chr8.4:48076303-48076322 None:intergenic 20.0%
!!! ATTATATGTCTATTTTGTCA+TGG - chr8.4:48075887-48075906 MS.gene34215:CDS 20.0%
!!! ATTATTTTTAATTCAGTGTC+CGG - chr8.4:48075370-48075389 MS.gene34215:CDS 20.0%
!!! TTTTGTTCACTATGTTAAAA+CGG - chr8.4:48075967-48075986 MS.gene34215:CDS 20.0%
!!! TTTTTCGAATATACATTTTC+CGG - chr8.4:48077189-48077208 MS.gene34215:intron 20.0%
! AGGAATAATTCAACTACAAT+AGG + chr8.4:48075545-48075564 None:intergenic 25.0%
! CATAATAGAAAAACCTCAAT+AGG + chr8.4:48075565-48075584 None:intergenic 25.0%
! CATGTTAAAGTAAGTAAAAG+AGG + chr8.4:48076131-48076150 None:intergenic 25.0%
! TAGTTAAATCCCAGTATAAT+TGG + chr8.4:48076195-48076214 None:intergenic 25.0%
! TAGTTGAATTATTCCTATTG+AGG - chr8.4:48075549-48075568 MS.gene34215:intron 25.0%
! TATGTTAAAACGGTGATAAA+CGG - chr8.4:48075977-48075996 MS.gene34215:CDS 25.0%
! TTAAAATGGTAAGTTTGTCT+TGG - chr8.4:48075610-48075629 MS.gene34215:CDS 25.0%
! TTCTAAACCAAATCATACTT+CGG + chr8.4:48076434-48076453 None:intergenic 25.0%
! TTGGAATTCTAAACTAGATA+AGG - chr8.4:48075786-48075805 MS.gene34215:CDS 25.0%
!!! TAGAAAATTTGCTAACACAA+CGG + chr8.4:48077624-48077643 None:intergenic 25.0%
!!! TTATTTTTAATTCAGTGTCC+GGG - chr8.4:48075371-48075390 MS.gene34215:CDS 25.0%
!!! TTTTGTCATGGTATTTTTTG+CGG - chr8.4:48075899-48075918 MS.gene34215:CDS 25.0%
AAATCCCAGTATAATTGGAA+GGG + chr8.4:48076190-48076209 None:intergenic 30.0%
AATACGTAAACAGATGAAGA+AGG - chr8.4:48076452-48076471 MS.gene34215:intron 30.0%
ACTTAAACTACAGGAAGATT+TGG - chr8.4:48075767-48075786 MS.gene34215:CDS 30.0%
AGTTTCAGCATCTTTAATGA+AGG + chr8.4:48076670-48076689 None:intergenic 30.0%
ATACCCTTTAATGTGCATAT+TGG + chr8.4:48077485-48077504 None:intergenic 30.0%
ATACGTAAACAGATGAAGAA+GGG - chr8.4:48076453-48076472 MS.gene34215:intron 30.0%
ATCATTGGCAAATGTAATTC+TGG + chr8.4:48076899-48076918 None:intergenic 30.0%
ATTATAAGAGCTTGAAGCAT+AGG + chr8.4:48077292-48077311 None:intergenic 30.0%
TAAATCCCAGTATAATTGGA+AGG + chr8.4:48076191-48076210 None:intergenic 30.0%
TCTTATAATTCAGAACGACA+AGG - chr8.4:48077303-48077322 MS.gene34215:CDS 30.0%
TGTAGTAAATGGTGAAATTC+CGG + chr8.4:48077211-48077230 None:intergenic 30.0%
TGTCCAATATGCACATTAAA+GGG - chr8.4:48077479-48077498 MS.gene34215:CDS 30.0%
TGTGAGTATAACTAAAGCTT+TGG - chr8.4:48077328-48077347 MS.gene34215:CDS 30.0%
TTGTCCAATATGCACATTAA+AGG - chr8.4:48077478-48077497 MS.gene34215:CDS 30.0%
TTGTCTTGGATATCAAATAG+CGG - chr8.4:48075624-48075643 MS.gene34215:CDS 30.0%
! AAATTTTCTAATATGCCGAG+GGG - chr8.4:48077634-48077653 MS.gene34215:CDS 30.0%
! CAAATTTTCTAATATGCCGA+GGG - chr8.4:48077633-48077652 MS.gene34215:CDS 30.0%
!!! GATTATTTCTGGTGTTTTCT+TGG - chr8.4:48077573-48077592 MS.gene34215:CDS 30.0%
!!! TAACATATTTTGGCAATACC+CGG + chr8.4:48075392-48075411 None:intergenic 30.0%
AAGATGCTGAAACTAGACAT+TGG - chr8.4:48076676-48076695 MS.gene34215:intron 35.0%
ACAAACCCTTCCAATTATAC+TGG - chr8.4:48076182-48076201 MS.gene34215:intron 35.0%
AGACAGTTAGGAACAGTATT+AGG + chr8.4:48077448-48077467 None:intergenic 35.0%
AGATTCATTTCCTCAAGAAG+CGG + chr8.4:48075247-48075266 None:intergenic 35.0%
AGTCATACAAGTAAGCATGT+GGG + chr8.4:48075156-48075175 None:intergenic 35.0%
ATTGCGATGTTATACAGCAA+AGG - chr8.4:48077044-48077063 MS.gene34215:intron 35.0%
CAAACCCTTCCAATTATACT+GGG - chr8.4:48076183-48076202 MS.gene34215:intron 35.0%
CAAGAATCCGAAGTATGATT+TGG - chr8.4:48076424-48076443 MS.gene34215:intron 35.0%
CGTAGTAGAAGTTGAATGAT+TGG + chr8.4:48074991-48075010 None:intergenic 35.0%
GAACAAAAATCACGACGAAA+CGG + chr8.4:48075955-48075974 None:intergenic 35.0%
GATACATCACCAAATGAAAG+TGG - chr8.4:48077409-48077428 MS.gene34215:CDS 35.0%
TACATTTGCCAATGATACTG+CGG - chr8.4:48076903-48076922 MS.gene34215:intron 35.0%
TAGACGTTTAATTCCAGTGA+GGG + chr8.4:48077022-48077041 None:intergenic 35.0%
TGTCTTGGATATCAAATAGC+GGG - chr8.4:48075625-48075644 MS.gene34215:CDS 35.0%
TTACGCTCCTGAAAATGAAT+CGG - chr8.4:48076969-48076988 MS.gene34215:intron 35.0%
TTCAAACTCTTCAAGGTAGT+TGG + chr8.4:48076805-48076824 None:intergenic 35.0%
! ACTTTCGACACTTTTCTGTA+CGG - chr8.4:48077250-48077269 MS.gene34215:intron 35.0%
! CGCATAGTATTTTGCGATTT+CGG - chr8.4:48075653-48075672 MS.gene34215:CDS 35.0%
! CTTTTTACATCTGAGGATCA+TGG - chr8.4:48075271-48075290 MS.gene34215:intron 35.0%
! GCAAATTTTCTAATATGCCG+AGG - chr8.4:48077632-48077651 MS.gene34215:CDS 35.0%
! TCTTGCTCTTTTTACATCTG+AGG - chr8.4:48075264-48075283 MS.gene34215:intron 35.0%
!! ATGCACATTAAAGGGTATCA+AGG - chr8.4:48077487-48077506 MS.gene34215:CDS 35.0%
!! GAAACTAGACATTGGTAATG+TGG - chr8.4:48076684-48076703 MS.gene34215:intron 35.0%
!! TACAGAAAAGTGTCGAAAGT+GGG + chr8.4:48077250-48077269 None:intergenic 35.0%
!! TGAGCTCTAGATGTAGTAAA+TGG + chr8.4:48077222-48077241 None:intergenic 35.0%
!!! CGCTTTTTTGTTGCATGATT+TGG - chr8.4:48076531-48076550 MS.gene34215:intron 35.0%
AAGTGAGACACAAGACAGTT+AGG + chr8.4:48077460-48077479 None:intergenic 40.0%
ACGAATGTTGCGTTAGATTG+CGG - chr8.4:48075681-48075700 MS.gene34215:CDS 40.0%
AGCTCCAATGTCATTCTCAA+CGG + chr8.4:48076833-48076852 None:intergenic 40.0%
ATCACCAAATGAAAGTGGCT+TGG - chr8.4:48077414-48077433 MS.gene34215:CDS 40.0%
ATGCTTACTTGTATGACTCG+TGG - chr8.4:48075158-48075177 MS.gene34215:CDS 40.0%
ATGTAATTCTGGTGAGGCTA+AGG + chr8.4:48076888-48076907 None:intergenic 40.0%
CGACAGACATTTCAGTAAAC+CGG + chr8.4:48075198-48075217 None:intergenic 40.0%
CTAGACGTTTAATTCCAGTG+AGG + chr8.4:48077023-48077042 None:intergenic 40.0%
CTTGGACCAAAAGAAGAAGT+GGG - chr8.4:48077591-48077610 MS.gene34215:CDS 40.0%
CTTGGAGGAGAATGAGTAAA+GGG + chr8.4:48076999-48077018 None:intergenic 40.0%
GACAGACATTTCAGTAAACC+GGG + chr8.4:48075197-48075216 None:intergenic 40.0%
GAGTCATACAAGTAAGCATG+TGG + chr8.4:48075157-48075176 None:intergenic 40.0%
GGTGAGTTTCAAACTCTTCA+AGG + chr8.4:48076812-48076831 None:intergenic 40.0%
TAATGTGGATGTGAATTCCG+TGG - chr8.4:48076699-48076718 MS.gene34215:intron 40.0%
TACGACTATAATCCCATCCT+CGG - chr8.4:48075007-48075026 MS.gene34215:CDS 40.0%
TCAAGGAGATTCTAGTGAGA+TGG - chr8.4:48077504-48077523 MS.gene34215:CDS 40.0%
TCTTGGACCAAAAGAAGAAG+TGG - chr8.4:48077590-48077609 MS.gene34215:CDS 40.0%
TGCTTACTTGTATGACTCGT+GGG - chr8.4:48075159-48075178 MS.gene34215:CDS 40.0%
TGGCAAATGTAATTCTGGTG+AGG + chr8.4:48076894-48076913 None:intergenic 40.0%
TGTAAGAAGAGACTGGTAGA+TGG - chr8.4:48077151-48077170 MS.gene34215:intron 40.0%
TGTAATGTACTCAGTCTCTG+CGG - chr8.4:48076601-48076620 MS.gene34215:intron 40.0%
! ACTTCTCCCACTTCTTCTTT+TGG + chr8.4:48077600-48077619 None:intergenic 40.0%
! AGGCTAAGGTATTTGAGATC+GGG + chr8.4:48076874-48076893 None:intergenic 40.0%
! GAGGCTAAGGTATTTGAGAT+CGG + chr8.4:48076875-48076894 None:intergenic 40.0%
! GCTAGCTATGTTTTGCAGAA+TGG - chr8.4:48077532-48077551 MS.gene34215:CDS 40.0%
! GTTTGTTTGTTAGCGTAGCA+TGG - chr8.4:48076502-48076521 MS.gene34215:intron 40.0%
! TTTTTGTCGCAGCCTTTTCT+CGG - chr8.4:48076020-48076039 MS.gene34215:intron 40.0%
!! AGTTCCAGGGTTTCTAAGAT+TGG + chr8.4:48076745-48076764 None:intergenic 40.0%
!! GTACAGAAAAGTGTCGAAAG+TGG + chr8.4:48077251-48077270 None:intergenic 40.0%
!! GTTCCAGGGTTTCTAAGATT+GGG + chr8.4:48076744-48076763 None:intergenic 40.0%
!! TCATTTGGTGATGTATCCTG+CGG + chr8.4:48077406-48077425 None:intergenic 40.0%
AACGACATTCCATCGACGTT+CGG - chr8.4:48075086-48075105 MS.gene34215:CDS 45.0%
AAGTAAGCATGTGGGTCTTC+GGG + chr8.4:48075148-48075167 None:intergenic 45.0%
AATGGTCCGTAACCGAGAAA+AGG + chr8.4:48076035-48076054 None:intergenic 45.0%
ACCTGATGGAGAGTAGCTTA+GGG + chr8.4:48075327-48075346 None:intergenic 45.0%
ACGGTGATAAACGGTAACGT+TGG - chr8.4:48075986-48076005 MS.gene34215:CDS 45.0%
ATGAGAGAACAGTGAGAGGA+AGG + chr8.4:48075303-48075322 None:intergenic 45.0%
ATGTATCCTGCGGGCAAATA+GGG + chr8.4:48077396-48077415 None:intergenic 45.0%
CAAGTAAGCATGTGGGTCTT+CGG + chr8.4:48075149-48075168 None:intergenic 45.0%
CTCACCGTTGAGAATGACAT+TGG - chr8.4:48076826-48076845 MS.gene34215:intron 45.0%
CTCGTCACGACAAGATAGAA+TGG + chr8.4:48076378-48076397 None:intergenic 45.0%
CTGAAATGTCTGTCGATGCT+TGG - chr8.4:48075203-48075222 MS.gene34215:CDS 45.0%
GAGAAAAGGCTGCGACAAAA+AGG + chr8.4:48076021-48076040 None:intergenic 45.0%
GCTTGGAGGAGAATGAGTAA+AGG + chr8.4:48077000-48077019 None:intergenic 45.0%
GGGTCACACTACTTTCTAGA+AGG - chr8.4:48076350-48076369 MS.gene34215:intron 45.0%
GTGTACGTGTAAGAAGAGAC+TGG - chr8.4:48077144-48077163 MS.gene34215:intron 45.0%
GTTTAATTCCAGTGAGGGCT+TGG + chr8.4:48077017-48077036 None:intergenic 45.0%
TACCTGATGGAGAGTAGCTT+AGG + chr8.4:48075328-48075347 None:intergenic 45.0%
TTGCCAATGATACTGCGGTT+GGG - chr8.4:48076908-48076927 MS.gene34215:intron 45.0%
TTTGCCAATGATACTGCGGT+TGG - chr8.4:48076907-48076926 MS.gene34215:intron 45.0%
! ACCAAATGAAAGTGGCTTGG+CGG - chr8.4:48077417-48077436 MS.gene34215:CDS 45.0%
! TGCCTTTTCTCCCATATCTC+TGG - chr8.4:48076765-48076784 MS.gene34215:intron 45.0%
!! CATTTGGTGATGTATCCTGC+GGG + chr8.4:48077405-48077424 None:intergenic 45.0%
!! GCGTTTGAAGTTTTCGGAGT+TGG + chr8.4:48075035-48075054 None:intergenic 45.0%
!! GGCGAAGCGTTTGAAGTTTT+CGG + chr8.4:48075041-48075060 None:intergenic 45.0%
!! GGGTCAACCGATTCATTTTC+AGG + chr8.4:48076979-48076998 None:intergenic 45.0%
!!! GGTATTTTTTGCGGCCGTAA+TGG - chr8.4:48075908-48075927 MS.gene34215:CDS 45.0%
AATTGTCCCTATTTGCCCGC+AGG - chr8.4:48077387-48077406 MS.gene34215:CDS 50.0%
ACGAAGCTTGGCCAGAGATA+TGG + chr8.4:48076779-48076798 None:intergenic 50.0%
ACGATATCTGCGTGAGACGA+GGG + chr8.4:48074951-48074970 None:intergenic 50.0%
AGAGAACAGTGAGAGGAAGG+CGG + chr8.4:48075300-48075319 None:intergenic 50.0%
CACGCAGATATCGTCATCTC+TGG - chr8.4:48074957-48074976 MS.gene34215:CDS 50.0%
CATTTCAGTAAACCGGGCAC+CGG + chr8.4:48075191-48075210 None:intergenic 50.0%
CGAAGCTTGGCCAGAGATAT+GGG + chr8.4:48076778-48076797 None:intergenic 50.0%
CTGCCCAATCTTAGAAACCC+TGG - chr8.4:48076738-48076757 MS.gene34215:intron 50.0%
GATGTATCCTGCGGGCAAAT+AGG + chr8.4:48077397-48077416 None:intergenic 50.0%
GCCAGAGAGAAGGATATCCA+CGG + chr8.4:48076719-48076738 None:intergenic 50.0%
GGCCAGAGATATGGGAGAAA+AGG + chr8.4:48076770-48076789 None:intergenic 50.0%
GGGAGAAAAGGCAAGTTCCA+GGG + chr8.4:48076758-48076777 None:intergenic 50.0%
GGTAGTTGGAACACGAAGCT+TGG + chr8.4:48076791-48076810 None:intergenic 50.0%
TAATTCCAGTGAGGGCTTGG+AGG + chr8.4:48077014-48077033 None:intergenic 50.0%
TCCGTGGATATCCTTCTCTC+TGG - chr8.4:48076715-48076734 MS.gene34215:intron 50.0%
TCTCTGCGGTGATATCGTTG+TGG - chr8.4:48076615-48076634 MS.gene34215:intron 50.0%
TGCCAATGATACTGCGGTTG+GGG - chr8.4:48076909-48076928 MS.gene34215:intron 50.0%
TGGGAGAAAAGGCAAGTTCC+AGG + chr8.4:48076759-48076778 None:intergenic 50.0%
! ACCGCCAAGCCACTTTCATT+TGG + chr8.4:48077421-48077440 None:intergenic 50.0%
! TCGCAGCCTTTTCTCGGTTA+CGG - chr8.4:48076026-48076045 MS.gene34215:intron 50.0%
!! GAGCACAGCGTTGACGAAAA+CGG + chr8.4:48075062-48075081 None:intergenic 50.0%
ACGCATCTTCCGAACGTCGA+TGG + chr8.4:48075098-48075117 None:intergenic 55.0%
AGATTGGGCAGCCAGAGAGA+AGG + chr8.4:48076729-48076748 None:intergenic 55.0%
ATTCTCCTCCAAGCCCTCAC+TGG - chr8.4:48077006-48077025 MS.gene34215:intron 55.0%
CGGAAGATGCGTCTCTCATG+TGG - chr8.4:48075106-48075125 MS.gene34215:CDS 55.0%
GACGATATCTGCGTGAGACG+AGG + chr8.4:48074952-48074971 None:intergenic 55.0%
GCCCTAAGCTACTCTCCATC+AGG - chr8.4:48075323-48075342 MS.gene34215:CDS 55.0%
GCCGCCGCAAAACACCATTA+CGG + chr8.4:48075925-48075944 None:intergenic 55.0%
GCGTTAGATTGCGGTTGTCG+CGG - chr8.4:48075690-48075709 MS.gene34215:CDS 55.0%
GGGCATGAGAGAACAGTGAG+AGG + chr8.4:48075307-48075326 None:intergenic 55.0%
GTCCCCAACCGCAGTATCAT+TGG + chr8.4:48076914-48076933 None:intergenic 55.0%
TATGACTCGTGGGTCCGATC+CGG - chr8.4:48075169-48075188 MS.gene34215:CDS 55.0%
TTCCTCAAGAAGCGGACCAG+CGG + chr8.4:48075239-48075258 None:intergenic 55.0%
! GCCGTAATGGTGTTTTGCGG+CGG - chr8.4:48075921-48075940 MS.gene34215:CDS 55.0%
! GCGGCCGTAATGGTGTTTTG+CGG - chr8.4:48075918-48075937 MS.gene34215:CDS 55.0%
GAAGCGGACCAGCGGCAATA+CGG + chr8.4:48075231-48075250 None:intergenic 60.0%
GCTTGGATCCGTATTGCCGC+TGG - chr8.4:48075220-48075239 MS.gene34215:intron 60.0%
! AGCTTTGGTGTGTGCGTGCG+TGG - chr8.4:48077343-48077362 MS.gene34215:CDS 60.0%
! AGTTTTCGGAGTTGGCGCCG+AGG + chr8.4:48075027-48075046 None:intergenic 60.0%
!! CAGTAAACCGGGCACCGGAT+CGG + chr8.4:48075186-48075205 None:intergenic 60.0%
TGCCGCTGGTCCGCTTCTTG+AGG - chr8.4:48075234-48075253 MS.gene34215:intron 65.0%
TGGCAGACAGCAGAGCCCCT+CGG + chr8.4:48077652-48077671 None:intergenic 65.0%
! TCGGAGTTGGCGCCGAGGAT+GGG + chr8.4:48075022-48075041 None:intergenic 65.0%
! TTCGGAGTTGGCGCCGAGGA+TGG + chr8.4:48075023-48075042 None:intergenic 65.0%
CGTGGGTCCGATCCGGTGCC+CGG - chr8.4:48075176-48075195 MS.gene34215:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 48074944 48077687 48074944 ID=MS.gene34215
chr8.4 mRNA 48074944 48077687 48074944 ID=MS.gene34215.t1;Parent=MS.gene34215
chr8.4 exon 48077287 48077687 48077287 ID=MS.gene34215.t1.exon1;Parent=MS.gene34215.t1
chr8.4 CDS 48077287 48077687 48077287 ID=cds.MS.gene34215.t1;Parent=MS.gene34215.t1
chr8.4 exon 48075566 48076016 48075566 ID=MS.gene34215.t1.exon2;Parent=MS.gene34215.t1
chr8.4 CDS 48075566 48076016 48075566 ID=cds.MS.gene34215.t1;Parent=MS.gene34215.t1
chr8.4 exon 48075307 48075471 48075307 ID=MS.gene34215.t1.exon3;Parent=MS.gene34215.t1
chr8.4 CDS 48075307 48075471 48075307 ID=cds.MS.gene34215.t1;Parent=MS.gene34215.t1
chr8.4 exon 48074944 48075222 48074944 ID=MS.gene34215.t1.exon4;Parent=MS.gene34215.t1
chr8.4 CDS 48074944 48075222 48074944 ID=cds.MS.gene34215.t1;Parent=MS.gene34215.t1
Gene Sequence

>MS.gene34215

ATGACCCTCGTCTCACGCAGATATCGTCATCTCTGGAAGCATCTCCAATCATTCAACTTCTACTACGACTATAATCCCATCCTCGGCGCCAACTCCGAAAACTTCAAACGCTTCGCCGTTTTCGTCAACGCTGTGCTCACTCAACGACATTCCATCGACGTTCGGAAGATGCGTCTCTCATGTGGTTACTTCAAGTCTCAACCCGAAGACCCACATGCTTACTTGTATGACTCGTGGGTCCGATCCGGTGCCCGGTTTACTGAAATGTCTGTCGATGCTTGGATCCGTATTGCCGCTGGTCCGCTTCTTGAGGAAATGAATCTTGCTCTTTTTACATCTGAGGATCATGGCTTCCGCCTTCCTCTCACTGTTCTCTCATGCCCTAAGCTACTCTCCATCAGGTATTTTCTTAATTTTTAATTATAGATTATTTTTAATTCAGTGTCCGGGTATTGCCAAAATATGTTAACACTAAGTTGAAATTATATGATACTAGTGTCAAGACAGACATTTTAGTTAATTGTATATTTTATTGCATTTGATTATAAATCTTTTGAAATATAACATGATCAAATTAAGATTTAACTAATATTTTGATCCTATTGTAGTTGAATTATTCCTATTGAGGTTTTTCTATTATGTGCAAATTTGCACATGTTAAGAAATTTAAAATGGTAAGTTTGTCTTGGATATCAAATAGCGGGTCGCGCGCATAGTATTTTGCGATTTCGGCTGTTACGAATGTTGCGTTAGATTGCGGTTGTCGCGGTGTGAATTAATCACAACATTGCACAACTCTTAAATAATGATAGTAACTTAAATAACTTAAACTACAGGAAGATTTGGAATTCTAAACTAGATAAGGAGATAACATTATGTAATTTATTATCAAATATGTACTAAAAATATGATTTTCATCTATTTCAATTGAAAAAATATTCAGATTATATGTCTATTTTGTCATGGTATTTTTTGCGGCCGTAATGGTGTTTTGCGGCGGCTGCAACACCGTTTCGTCGTGATTTTTGTTCACTATGTTAAAACGGTGATAAACGGTAACGTTGGCTATCTATACCTTTTTGTCGCAGCCTTTTCTCGGTTACGGACCATTTTTTAAAACCATGCATTTCATATTAAATATATAACTTTTTAATAAATAATTGATGTTTTTCAATAAGAAATTACCTCTTTTACTTACTTTAACATGTGCATAACATGTGCAATATTGCACATGTTAGACAAACCCTTCCAATTATACTGGGATTTAACTAATTTTTTTTTATAAAAAGATAAAATTGTAACTACAATAGTTTAAATGAATTGTATAATTGGGAGAATGAAAAAGTTGAGACAAATCCGTTTTCCACTTTAAATATTGTTATGGATTATAGATATACTTAGATGATGGGTCACACTACTTTCTAGAAGGATCCATTCTATCTTGTCGTGACGAGTCATTATACTGTTATGTTTATGTTAGCAAGAATCCGAAGTATGATTTGGTTTAGAATACGTAAACAGATGAAGAAGGGAATAACATACAAGAATATTGAAGAAAGTTTGTTTGTTAGCGTAGCATGGTTATCTCGCTTTTTTGTTGCATGATTTGGTTTCATCTTAATATCTTATTGCACTAAGAACCTTATAATAACATTTATGTAATGTACTCAGTCTCTGCGGTGATATCGTTGTGGAATTAGCACAATCTTCAGCAGTTTATTTGCCTTCATTAAAGATGCTGAAACTAGACATTGGTAATGTGGATGTGAATTCCGTGGATATCCTTCTCTCTGGCTGCCCAATCTTAGAAACCCTGGAACTTGCCTTTTCTCCCATATCTCTGGCCAAGCTTCGTGTTCCAACTACCTTGAAGAGTTTGAAACTCACCGTTGAGAATGACATTGGAGCTTGTGTTGAGATTGATGCACCCGATCTCAAATACCTTAGCCTCACCAGAATTACATTTGCCAATGATACTGCGGTTGGGGACTTGCACAACGTGAAAGAAGCATATCTTGATGTATTTTACGCTCCTGAAAATGAATCGGTTGACCCTTTACTCATTCTCCTCCAAGCCCTCACTGGAATTAAACGTCTAGTATTGCGATGTTATACAGCAAAGGTGAGTGAAATTTTCTTTATCTATATTTTTCATTATATATTTTGTAGTTTTGTGCTAATATTACTTACTGTTGTTGTTGTGTACGTGTAAGAAGAGACTGGTAGATGGTCAACCTATTATAAATTTTTCGAATATACATTTTCCGGAATTTCACCATTTACTACATCTAGAGCTCATTCTTCCCACTTTCGACACTTTTCTGTACGGTGTACTTCAGAAATGTCCTATGCTTCAAGCTCTTATAATTCAGAACGACAAGGTTTGTGAGTATAACTAAAGCTTTGGTGTGTGCGTGCGTGGTTTTTGTTTTCATCTTACGTCAATTGTCCCTATTTGCCCGCAGGATACATCACCAAATGAAAGTGGCTTGGCGGTGAAGCCTAATACTGTTCCTAACTGTCTTGTGTCTCACTTGTCCAATATGCACATTAAAGGGTATCAAGGAGATTCTAGTGAGATGGAATTTGCTAGCTATGTTTTGCAGAATGGACTTGTTTTAAAGAAAATGATTATTTCTGGTGTTTTCTTGGACCAAAAGAAGAAGTGGGAGAAGTACCGTTGTGTTAGCAAATTTTCTAATATGCCGAGGGGCTCTGCTGTCTGCCAAGTTACATTTGACTGA
Protein sequence

>MS.gene34215.t1

MTLVSRRYRHLWKHLQSFNFYYDYNPILGANSENFKRFAVFVNAVLTQRHSIDVRKMRLSCGYFKSQPEDPHAYLYDSWVRSGARFTEMSVDAWIRIAAGPLLEEMNLALFTSEDHGFRLPLTVLSCPKLLSISLCGDIVVELAQSSAVYLPSLKMLKLDIGNVDVNSVDILLSGCPILETLELAFSPISLAKLRVPTTLKSLKLTVENDIGACVEIDAPDLKYLSLTRITFANDTAVGDLHNVKEAYLDVFYAPENESVDPLLILLQALTGIKRLVLRCYTAKRLVDGQPIINFSNIHFPEFHHLLHLELILPTFDTFLYGVLQKCPMLQALIIQNDKDTSPNESGLAVKPNTVPNCLVSHLSNMHIKGYQGDSSEMEFASYVLQNGLVLKKMIISGVFLDQKKKWEKYRCVSKFSNMPRGSAVCQVTFD