AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72959

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72959.t1 MTR_8g468810 79.036 415 84 2 1 414 51 463 0.0 655
MS.gene72959.t1 MTR_8g468810 81.734 323 56 2 1 322 51 371 0.0 521
MS.gene72959.t1 MTR_8g058970 62.587 433 143 3 1 414 45 477 0.0 517
MS.gene72959.t1 MTR_8g468810 82.510 263 44 1 1 263 51 311 1.82e-151 432
MS.gene72959.t1 MTR_8g468810 82.510 263 44 1 1 263 51 311 3.15e-151 431
MS.gene72959.t1 MTR_8g468810 82.510 263 44 1 1 263 51 311 5.56e-151 432
MS.gene72959.t1 MTR_2g083840 54.306 418 180 6 1 414 47 457 1.47e-141 412
MS.gene72959.t1 MTR_8g062270 47.552 429 193 11 1 413 41 453 1.19e-115 345
MS.gene72959.t1 MTR_4g032730 47.045 440 188 9 3 413 43 466 2.47e-114 342
MS.gene72959.t1 MTR_5g092960 49.767 430 182 13 3 413 47 461 1.12e-109 330
MS.gene72959.t1 MTR_8g468840 47.619 420 200 9 2 412 40 448 2.19e-109 329
MS.gene72959.t1 MTR_8g468760 48.712 427 163 13 1 413 34 418 5.49e-107 322
MS.gene72959.t1 MTR_8g467800 48.009 427 189 12 2 414 46 453 1.47e-106 322
MS.gene72959.t1 MTR_8g014260 43.735 423 211 15 4 414 44 451 3.49e-97 298
MS.gene72959.t1 MTR_8g445780 44.536 366 181 9 4 364 41 389 2.18e-82 258
MS.gene72959.t1 MTR_5g092960 50.599 334 142 10 3 322 47 371 1.36e-79 251
MS.gene72959.t1 MTR_8g469020 38.337 433 232 12 2 414 34 451 4.58e-78 249
MS.gene72959.t1 MTR_8g058965 39.019 428 235 10 2 414 40 456 3.93e-76 244
MS.gene72959.t1 MTR_8g070950 37.875 433 211 15 1 414 41 434 1.62e-70 229
MS.gene72959.t1 MTR_4g050250 40.576 382 191 10 1 356 47 418 5.20e-68 222
MS.gene72959.t1 MTR_8g467800 51.852 270 118 6 2 266 46 308 1.08e-67 218
MS.gene72959.t1 MTR_8g062080 36.706 425 230 14 1 414 49 445 7.84e-64 212
MS.gene72959.t1 MTR_8g062010 36.993 419 236 12 1 414 35 430 8.12e-64 211
MS.gene72959.t1 MTR_8g014160 33.886 422 207 11 2 414 67 425 1.08e-59 200
MS.gene72959.t1 MTR_4g024710 42.193 301 160 8 117 414 58 347 2.13e-58 194
MS.gene72959.t1 MTR_8g062310 35.817 416 221 14 4 414 54 428 1.43e-57 195
MS.gene72959.t1 MTR_8g062300 34.744 449 235 16 1 414 52 477 2.64e-54 187
MS.gene72959.t1 MTR_4g024745 41.259 286 154 8 115 397 10 284 3.40e-48 166
MS.gene72959.t1 MTR_8g014210 43.568 241 126 6 174 414 1 231 2.16e-46 159
MS.gene72959.t1 MTR_8g467790 34.417 369 176 13 1 354 174 491 8.08e-45 162
MS.gene72959.t1 MTR_8g077460 41.969 193 106 4 4 191 38 229 1.01e-36 138
MS.gene72959.t1 MTR_8g077460 33.209 268 150 9 155 414 129 375 3.17e-29 117
MS.gene72959.t1 MTR_5g021550 50.000 116 55 3 4 116 47 162 2.50e-27 107
MS.gene72959.t1 MTR_8g014290 43.548 124 59 5 4 117 38 160 1.19e-23 96.7
MS.gene72959.t1 MTR_2g086020 28.297 417 194 14 1 414 1 315 1.38e-23 100
MS.gene72959.t1 MTR_4g056040 29.630 405 237 16 13 388 65 450 5.76e-23 101
MS.gene72959.t1 MTR_5g080710 27.594 424 266 15 2 413 59 453 6.21e-23 100
MS.gene72959.t1 MTR_4g113810 36.471 170 101 5 57 221 1 168 1.70e-21 91.3
MS.gene72959.t1 MTR_4g055990 28.610 374 224 14 41 389 106 461 1.04e-20 94.4
MS.gene72959.t1 MTR_4g055990 28.610 374 224 14 41 389 106 461 1.16e-20 94.4
MS.gene72959.t1 MTR_4g056070 29.600 375 213 16 17 364 86 436 1.76e-20 93.2
MS.gene72959.t1 MTR_5g084070 27.273 385 238 14 2 376 39 391 1.07e-19 90.9
MS.gene72959.t1 MTR_8g014220 42.975 121 57 5 46 161 47 160 1.99e-17 79.7
MS.gene72959.t1 MTR_4g056030 28.493 365 211 15 41 378 69 410 2.72e-17 84.0
MS.gene72959.t1 MTR_4g056020 28.371 356 225 14 41 379 69 411 2.37e-16 80.9
MS.gene72959.t1 MTR_4g024720 36.567 134 62 6 4 116 45 176 5.12e-16 76.6
MS.gene72959.t1 MTR_4g055580 27.671 365 208 14 44 378 199 537 2.06e-15 78.6
MS.gene72959.t1 MTR_1g038710 26.705 352 227 11 48 378 85 426 4.01e-15 77.4
MS.gene72959.t1 MTR_1g041245 32.544 169 97 4 2 166 37 192 2.30e-14 74.7
MS.gene72959.t1 MTR_1g041245 32.609 138 80 4 286 414 253 386 9.08e-11 63.5
MS.gene72959.t1 MTR_8g014230 48.000 75 35 2 330 404 25 95 3.41e-12 63.2
MS.gene72959.t1 MTR_2g072720 23.666 431 274 14 2 401 65 471 1.66e-11 66.2
MS.gene72959.t1 MTR_7g115270 22.353 425 252 14 2 411 38 399 4.17e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72959.t1 AT4G26340 28.337 427 257 16 2 414 28 419 1.41e-26 110
MS.gene72959.t1 AT1G78750 26.682 431 276 15 2 411 44 455 8.65e-23 100
MS.gene72959.t1 AT1G78750 26.682 431 276 15 2 411 44 455 8.65e-23 100
MS.gene72959.t1 AT5G38590 27.436 390 231 14 2 376 28 380 3.53e-20 92.4
MS.gene72959.t1 AT1G66300 28.409 440 242 21 3 411 56 453 6.72e-20 91.7
MS.gene72959.t1 AT4G13985 26.018 442 267 18 1 411 44 456 8.85e-20 91.7
MS.gene72959.t1 AT1G51370 27.315 432 248 23 2 411 45 432 2.01e-19 90.1
MS.gene72959.t1 AT5G56420 25.229 436 258 18 2 414 32 422 4.57e-19 89.0
MS.gene72959.t1 AT5G56420 25.229 436 258 18 2 414 32 422 4.57e-19 89.0
MS.gene72959.t1 AT5G56420 25.229 436 258 18 2 414 32 422 4.57e-19 89.0
MS.gene72959.t1 AT1G66290 27.064 436 252 21 2 411 55 450 8.26e-19 88.6
MS.gene72959.t1 AT5G56370 26.728 434 255 19 2 413 28 420 2.35e-18 87.0
MS.gene72959.t1 AT5G56370 26.728 434 255 19 2 413 28 420 2.35e-18 87.0
MS.gene72959.t1 AT3G50710 25.866 433 266 17 3 414 29 427 3.39e-18 86.7
MS.gene72959.t1 AT5G22730 27.815 453 241 22 2 411 54 463 6.97e-18 85.9
MS.gene72959.t1 AT5G22730 27.815 453 241 22 2 411 54 463 6.97e-18 85.9
MS.gene72959.t1 AT1G32375 26.157 432 261 17 3 414 29 422 9.71e-18 85.1
MS.gene72959.t1 AT1G32375 26.157 432 261 17 3 414 29 422 9.71e-18 85.1
MS.gene72959.t1 AT5G38570 26.128 421 227 16 3 413 29 375 1.28e-17 84.3
MS.gene72959.t1 AT4G10400 25.882 425 258 20 2 413 28 408 2.58e-17 83.6
MS.gene72959.t1 AT4G10400 25.882 425 258 20 2 413 28 408 2.58e-17 83.6
MS.gene72959.t1 AT4G10400 25.882 425 258 20 2 413 28 408 2.58e-17 83.6
MS.gene72959.t1 AT3G54910 26.923 390 241 13 36 414 17 373 5.58e-17 82.4
MS.gene72959.t1 AT3G54910 26.923 390 241 13 36 414 17 373 5.58e-17 82.4
MS.gene72959.t1 AT3G54910 26.923 390 241 13 36 414 17 373 5.58e-17 82.4
MS.gene72959.t1 AT1G06630 29.496 278 169 10 2 266 38 301 1.56e-16 80.1
MS.gene72959.t1 AT5G38590 30.126 239 146 9 2 233 28 252 1.59e-16 80.1
MS.gene72959.t1 AT1G06630 28.228 333 206 12 2 319 38 352 1.73e-16 80.9
MS.gene72959.t1 AT1G06630 28.228 333 206 12 2 319 38 352 1.73e-16 80.9
MS.gene72959.t1 AT1G06630 28.144 334 207 12 2 320 47 362 2.32e-16 80.9
MS.gene72959.t1 AT1G06630 28.144 334 207 12 2 320 38 353 2.42e-16 80.9
MS.gene72959.t1 AT1G06630 28.144 334 207 12 2 320 38 353 2.42e-16 80.9
MS.gene72959.t1 AT1G06630 28.144 334 207 12 2 320 38 353 2.42e-16 80.9
MS.gene72959.t1 AT1G06630 28.144 334 207 12 2 320 38 353 2.42e-16 80.9
MS.gene72959.t1 AT1G06630 28.144 334 207 12 2 320 38 353 3.05e-16 80.9
MS.gene72959.t1 AT5G60610 25.935 401 234 15 2 380 28 387 3.47e-16 80.1
MS.gene72959.t1 AT5G60610 25.935 401 234 15 2 380 28 387 3.47e-16 80.1
MS.gene72959.t1 AT5G22720 27.174 460 243 22 2 413 49 464 1.49e-15 78.6
MS.gene72959.t1 AT5G22670 29.759 373 213 19 30 376 58 407 2.33e-15 78.2
MS.gene72959.t1 AT1G55660 24.130 431 286 17 2 411 79 489 4.03e-15 77.4
MS.gene72959.t1 AT3G58920 23.729 472 270 18 2 414 28 468 6.58e-15 76.6
MS.gene72959.t1 AT4G10420 25.723 346 222 13 77 413 2 321 1.69e-14 74.3
MS.gene72959.t1 AT5G56700 26.862 376 230 17 14 375 2 346 1.82e-14 74.7
MS.gene72959.t1 AT5G53840 24.764 424 275 17 2 411 44 437 3.59e-14 74.3
MS.gene72959.t1 AT5G44950 27.970 404 239 20 2 380 30 406 6.62e-14 73.6
MS.gene72959.t1 AT3G52680 25.172 437 267 18 2 414 47 447 6.69e-14 73.6
MS.gene72959.t1 AT5G56440 24.654 434 273 18 2 413 28 429 1.08e-13 72.8
MS.gene72959.t1 AT2G26030 26.829 451 252 23 2 411 30 443 1.74e-13 72.4
MS.gene72959.t1 AT5G56810 26.914 431 254 18 2 411 41 431 1.86e-13 72.0
MS.gene72959.t1 AT5G38590 30.808 198 125 6 41 233 27 217 2.15e-13 70.1
MS.gene72959.t1 AT2G26860 25.899 417 237 19 2 385 28 405 2.52e-13 71.6
MS.gene72959.t1 AT2G26860 25.899 417 237 19 2 385 28 405 2.52e-13 71.6
MS.gene72959.t1 AT1G55660 27.778 216 144 7 2 212 79 287 3.79e-13 70.9
MS.gene72959.t1 AT1G22000 25.459 381 221 15 2 356 55 398 4.17e-13 71.2
MS.gene72959.t1 AT5G56390 24.709 429 271 14 3 413 30 424 4.64e-13 70.9
MS.gene72959.t1 AT4G00315 24.884 430 284 17 2 411 28 438 6.00e-13 70.5
MS.gene72959.t1 AT1G13780 26.585 410 248 19 2 380 36 423 6.37e-13 70.5
MS.gene72959.t1 AT5G50270 25.123 406 254 17 3 389 30 404 2.43e-12 68.6
MS.gene72959.t1 AT4G14096 26.316 361 230 13 2 344 34 376 3.29e-12 68.6
MS.gene72959.t1 AT4G14096 26.316 361 230 13 2 344 34 376 3.29e-12 68.6
MS.gene72959.t1 AT3G49480 26.115 314 194 12 80 375 114 407 3.89e-12 68.2
MS.gene72959.t1 AT3G26920 27.954 347 216 15 78 413 16 339 5.43e-12 67.0
MS.gene72959.t1 AT3G26920 27.954 347 216 15 78 413 16 339 5.43e-12 67.0
MS.gene72959.t1 AT5G27750 24.289 457 272 18 2 414 31 457 7.93e-12 67.0
MS.gene72959.t1 AT3G54160 25.359 418 254 17 24 412 36 424 7.98e-12 67.0
MS.gene72959.t1 AT5G56400 24.771 436 261 19 4 414 62 455 9.14e-12 67.0
MS.gene72959.t1 AT1G66310 25.455 440 253 22 2 411 45 439 1.14e-11 66.6
MS.gene72959.t1 AT3G49480 26.829 287 176 11 103 375 1 267 1.31e-11 65.5
MS.gene72959.t1 AT3G49030 25.058 431 241 17 2 413 47 414 2.63e-11 65.5
MS.gene72959.t1 AT3G58860 23.227 409 282 13 2 397 33 422 4.26e-11 65.1
MS.gene72959.t1 AT3G10750 28.000 300 169 14 3 295 2 261 4.53e-11 63.9
MS.gene72959.t1 AT4G09920 29.091 275 161 16 2 264 28 280 4.83e-11 63.9
MS.gene72959.t1 AT4G09920 29.091 275 161 16 2 264 28 280 4.83e-11 63.9
MS.gene72959.t1 AT5G56370 27.372 274 168 11 2 269 28 276 6.32e-11 63.5
MS.gene72959.t1 AT4G09920 28.832 274 167 15 2 262 28 286 8.16e-11 63.2
MS.gene72959.t1 AT4G09920 28.832 274 167 15 2 262 28 286 8.16e-11 63.2
MS.gene72959.t1 AT4G09920 28.832 274 167 15 2 262 28 286 8.16e-11 63.2
MS.gene72959.t1 AT4G00160 25.694 432 263 17 2 410 42 438 8.30e-11 63.9

Find 88 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGACCTATATCCACTTTAA+AGG 0.185801 8.3:+41842916 MS.gene72959:CDS
CGGTGAGATCTTTGTAGAAT+TGG 0.284338 8.3:+41842047 MS.gene72959:CDS
ACGTACCAAAAGAGATTATT+AGG 0.292927 8.3:+41842583 MS.gene72959:intron
CCTCTTCTCGTCTGAGGATC+AGG 0.298567 8.3:+41841198 MS.gene72959:CDS
CTTGATGTAGTCTCCTCTTC+TGG 0.309388 8.3:+41842384 MS.gene72959:CDS
CACCACATCAATTTGGTCTT+AGG 0.331226 8.3:+41842854 MS.gene72959:CDS
AAGTGGGATGCAAGACAATT+AGG 0.332413 8.3:-41842895 None:intergenic
CTGAATCTTTGGCCAAGCTT+AGG 0.379532 8.3:+41842202 MS.gene72959:CDS
GGCAGAGAGAAGGATATTAA+TGG 0.390004 8.3:-41842142 None:intergenic
TACGAAGGACCCGCGACATT+CGG 0.394870 8.3:+41841065 MS.gene72959:CDS
AGCAAAGTCTTTGAAATGTT+CGG 0.401003 8.3:-41841017 None:intergenic
AGTAAATTCAAAACTCTTCA+AGG 0.409402 8.3:-41842235 None:intergenic
GGTGAGATCTTTGTAGAATT+GGG 0.418728 8.3:+41842048 MS.gene72959:CDS
ACTCTCCTCCAAGCGCTCTC+TGG 0.418872 8.3:+41842432 MS.gene72959:CDS
TTCGGCCTCGCCGTCGACGC+CGG 0.419368 8.3:-41840999 None:intergenic
TGAATCTTTGGCCAAGCTTA+GGG 0.420723 8.3:+41842203 MS.gene72959:CDS
CGTGGATTCGCACCGCCGTT+GGG 0.424276 8.3:+41841149 MS.gene72959:CDS
GATACAGCACCACATCAATT+TGG 0.425006 8.3:+41842847 MS.gene72959:CDS
AGCCTCACTGATATCACTAT+TGG 0.428235 8.3:+41842315 MS.gene72959:CDS
TCGGCCTCGCCGTCGACGCC+GGG 0.429312 8.3:-41840998 None:intergenic
CTCATAGGTATCTTCATCTC+TGG 0.437215 8.3:+41840936 MS.gene72959:CDS
GGCCAAAGATTCAGTTGAAA+AGG 0.438596 8.3:-41842193 None:intergenic
TGAAGTTATCAAGTTGAGAT+TGG 0.445049 8.3:-41841108 None:intergenic
AGAGGAGGTTAGTGCATGAT+AGG 0.446173 8.3:-41841243 None:intergenic
ATTATAAGAGCTTCAAGTAT+AGG 0.447586 8.3:-41842724 None:intergenic
AGTTTCAAAGTTTCTAAGAC+TGG 0.448465 8.3:-41842168 None:intergenic
ATTTAACTCCAGAGAGCGCT+TGG 0.458373 8.3:-41842440 None:intergenic
TTGGCGATGCCACTTCTGTT+GGG 0.460446 8.3:+41842334 MS.gene72959:CDS
ATCCTCTATATCCTTTAAAG+TGG 0.461419 8.3:-41842927 None:intergenic
ATCCACTTTAAAGGATATAG+AGG 0.462211 8.3:+41842925 MS.gene72959:CDS
TCAAAACTCTTCAAGGAAGA+TGG 0.465651 8.3:-41842228 None:intergenic
GCACTAACCTCCTCTCCATC+AGG 0.467643 8.3:+41841251 MS.gene72959:CDS
CATCGTAGAAATCAAAAGCT+TGG 0.471473 8.3:-41840967 None:intergenic
CAATGAGCCTTGTCTCTCAT+AGG 0.474674 8.3:+41840921 None:intergenic
CTTGGAGGAGAGTGAGTAAA+GGG 0.475202 8.3:-41842422 None:intergenic
ACGTGGATTCGCACCGCCGT+TGG 0.482549 8.3:+41841148 MS.gene72959:CDS
ATTGGCGATGCCACTTCTGT+TGG 0.491458 8.3:+41842333 MS.gene72959:CDS
TGTAGAATTGGGACAATCAT+CGG 0.493103 8.3:+41842059 MS.gene72959:CDS
ATATCCTTTAAAGTGGATAT+AGG 0.498926 8.3:-41842920 None:intergenic
CAAAGTTTCTAAGACTGGAC+AGG 0.505834 8.3:-41842163 None:intergenic
TTAAAGGATATAGAGGATGT+TGG 0.507552 8.3:+41842932 MS.gene72959:CDS
TAGAGGATGTTGGCTTGAGA+TGG 0.521759 8.3:+41842942 MS.gene72959:CDS
AGTTGTAATCTCTTCAATGA+TGG 0.525654 8.3:-41842093 None:intergenic
TAGTGATATCAGTGAGGCTA+AGG 0.528202 8.3:-41842311 None:intergenic
TTCTACGATGAGTACTCTCC+CGG 0.531961 8.3:+41840980 MS.gene72959:CDS
TACAGAAAAGCGTCGAAAGT+AGG 0.534200 8.3:-41842682 None:intergenic
CTCTTCTCGTCTGAGGATCA+GGG 0.539018 8.3:+41841199 MS.gene72959:CDS
ATCCTCAAGGTACGGCCCAA+CGG 0.545220 8.3:-41841164 None:intergenic
CGCCGTTGGGCCGTACCTTG+AGG 0.554002 8.3:+41841162 MS.gene72959:CDS
CCTGATCCTCAGACGAGAAG+AGG 0.554039 8.3:-41841198 None:intergenic
ACGCATCTTCCGAATGTCGC+GGG 0.555891 8.3:-41841074 None:intergenic
GAGTACTCTCCCGGCGTCGA+CGG 0.563225 8.3:+41840989 MS.gene72959:CDS
TGTAATGCACACAGTCTCTA+CGG 0.564381 8.3:+41842027 MS.gene72959:intron
TCTCGACCTCTTCTCGTCTG+AGG 0.568024 8.3:+41841192 MS.gene72959:CDS
AACAGATTCACTTCCAGAAG+AGG 0.569559 8.3:-41842397 None:intergenic
TAACTCCAGAGAGCGCTTGG+AGG 0.571531 8.3:-41842437 None:intergenic
GCTTGGAGGAGAGTGAGTAA+AGG 0.575040 8.3:-41842423 None:intergenic
TAAAGTGGATATAGGTCAAG+TGG 0.575798 8.3:-41842912 None:intergenic
GGAAGATGGAACCCTAAGCT+TGG 0.579963 8.3:-41842214 None:intergenic
GTGCAAGTTCCCAACAGAAG+TGG 0.580230 8.3:-41842343 None:intergenic
AGACTGGACAGGCAGAGAGA+AGG 0.581233 8.3:-41842152 None:intergenic
CTGAACGCTCTGTTGACACG+TGG 0.585988 8.3:+41841131 MS.gene72959:CDS
AGATGAAGATCCTCAAGGTA+CGG 0.599815 8.3:-41841172 None:intergenic
AAGAGATTACAACTAGACAT+TGG 0.609472 8.3:+41842102 MS.gene72959:CDS
CGGAAGATGCGTCTCTCTTG+CGG 0.610368 8.3:+41841085 MS.gene72959:CDS
GAACTTAGTCATATTTAACT+CGG 0.618369 8.3:-41843107 None:intergenic
GGGCTTCAAACTCCCTCTCG+CGG 0.620327 8.3:+41841219 MS.gene72959:CDS
ACAACTAGACATTGGTAATG+TGG 0.620834 8.3:+41842110 MS.gene72959:CDS
AAGGTAACCTGATGGAGAGG+AGG 0.620939 8.3:-41841258 None:intergenic
AGGCTAAGGTACTTGAGACC+AGG 0.622331 8.3:-41842297 None:intergenic
CTCTCCCGGCGTCGACGGCG+AGG 0.630012 8.3:+41840994 MS.gene72959:CDS
CTCAAGGTACGGCCCAACGG+CGG 0.630217 8.3:-41841161 None:intergenic
GACGCATCTTCCGAATGTCG+CGG 0.638940 8.3:-41841075 None:intergenic
AAAGTGGATATAGGTCAAGT+GGG 0.643217 8.3:-41842911 None:intergenic
ACGGCTTTCTCTCTTTACGA+AGG 0.645259 8.3:+41841050 MS.gene72959:CDS
GTTTAGTTGTAATACAACAA+TGG 0.648312 8.3:+41842470 MS.gene72959:CDS
TCTTATAATTCAGAATGACA+AGG 0.648619 8.3:+41842738 MS.gene72959:CDS
TGTTGGGAACTTGCACAACG+TGG 0.655593 8.3:+41842350 MS.gene72959:CDS
TGCATGATAGGACCGCGAGA+GGG 0.662917 8.3:-41841231 None:intergenic
GTCCTAAGACCAAATTGATG+TGG 0.669559 8.3:-41842856 None:intergenic
GTGCATGATAGGACCGCGAG+AGG 0.674731 8.3:-41841232 None:intergenic
TGTCTTGAGATAGACACACC+TGG 0.677521 8.3:+41842279 MS.gene72959:CDS
GAAGATACCTATGAGAGACA+AGG 0.684686 8.3:-41840928 None:intergenic
GGTCGAGATGAAGATCCTCA+AGG 0.701781 8.3:-41841177 None:intergenic
TGGGAACTTGCACAACGTGG+AGG 0.706891 8.3:+41842353 MS.gene72959:CDS
CGCCAATAGTGATATCAGTG+AGG 0.714173 8.3:-41842317 None:intergenic
CGGCAGACAGTAGATCCCCG+TGG 0.745934 8.3:-41843087 None:intergenic
AAAATTTCGTATATGCCACG+GGG 0.751789 8.3:+41843072 MS.gene72959:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAAGGAATATATAATCA+TGG - chr8.3:41842515-41842534 None:intergenic 15.0%
!!! ATGTTTTTTTTAGAAAGTTT+AGG - chr8.3:41841626-41841645 None:intergenic 15.0%
!!! TTGTCATATCTTTTATTAAT+TGG - chr8.3:41841765-41841784 None:intergenic 15.0%
!! ACTAAAATACTCAAATATGA+AGG - chr8.3:41841297-41841316 None:intergenic 20.0%
!! CTAAAATACTCAAATATGAA+GGG - chr8.3:41841296-41841315 None:intergenic 20.0%
!! GAAGACTACTAAATATATAA+AGG - chr8.3:41842530-41842549 None:intergenic 20.0%
!! TGAAGGGAAAAAAAAAAAAA+AGG - chr8.3:41841280-41841299 None:intergenic 20.0%
!!! ACGAAATTTTTTTGAGAAAA+TGG - chr8.3:41843062-41843081 None:intergenic 20.0%
!!! TTTTTATTCTACTATTGTAC+TGG + chr8.3:41841874-41841893 MS.gene72959:intron 20.0%
! AAAAAAAAAAGGTAACCTGA+TGG - chr8.3:41841269-41841288 None:intergenic 25.0%
! AAAAAATTTCGTATATGCCA+CGG + chr8.3:41843070-41843089 MS.gene72959:CDS 25.0%
! AGTAAATTCAAAACTCTTCA+AGG - chr8.3:41842238-41842257 None:intergenic 25.0%
! ATTATAAGAGCTTCAAGTAT+AGG - chr8.3:41842727-41842746 None:intergenic 25.0%
! GTTTAGTTGTAATACAACAA+TGG + chr8.3:41842470-41842489 MS.gene72959:CDS 25.0%
! TCTTATAATTCAGAATGACA+AGG + chr8.3:41842738-41842757 MS.gene72959:CDS 25.0%
! TGTTATATGAAAATAACTCG+AGG + chr8.3:41841658-41841677 MS.gene72959:intron 25.0%
!!! ATATCCTTTAAAGTGGATAT+AGG - chr8.3:41842923-41842942 None:intergenic 25.0%
!!! GTGTTGTTTGTTTGATTATT+TGG + chr8.3:41842800-41842819 MS.gene72959:intron 25.0%
!!! TTTTTCGAAAATGCACTTTT+CGG + chr8.3:41842624-41842643 MS.gene72959:CDS 25.0%
AAAAATTTCGTATATGCCAC+GGG + chr8.3:41843071-41843090 MS.gene72959:CDS 30.0%
AAAATAACTCGAGGAAAACT+AGG + chr8.3:41841667-41841686 MS.gene72959:intron 30.0%
AAGAGATTACAACTAGACAT+TGG + chr8.3:41842102-41842121 MS.gene72959:CDS 30.0%
ACGTACCAAAAGAGATTATT+AGG + chr8.3:41842583-41842602 MS.gene72959:intron 30.0%
AGTTGTAATCTCTTCAATGA+TGG - chr8.3:41842096-41842115 None:intergenic 30.0%
TACAGAGAGTTAGGTATAAT+GGG + chr8.3:41841341-41841360 MS.gene72959:intron 30.0%
TTAAAGGATATAGAGGATGT+TGG + chr8.3:41842932-41842951 MS.gene72959:CDS 30.0%
TTAGAAAGTTTAGGTGAGTT+GGG - chr8.3:41841617-41841636 None:intergenic 30.0%
TTGACCTATATCCACTTTAA+AGG + chr8.3:41842916-41842935 MS.gene72959:CDS 30.0%
TTTAGAAAGTTTAGGTGAGT+TGG - chr8.3:41841618-41841637 None:intergenic 30.0%
! AGACAATTAGGAACACTTTT+CGG - chr8.3:41842886-41842905 None:intergenic 30.0%
! ATCCACTTTAAAGGATATAG+AGG + chr8.3:41842925-41842944 MS.gene72959:CDS 30.0%
! ATCCTCTATATCCTTTAAAG+TGG - chr8.3:41842930-41842949 None:intergenic 30.0%
! GACTACCTAATAATCTCTTT+TGG - chr8.3:41842591-41842610 None:intergenic 30.0%
! GCTAGTTATGTTTTGCATTA+TGG + chr8.3:41842970-41842989 MS.gene72959:CDS 30.0%
! TTCCTTTTCAACTGAATCTT+TGG + chr8.3:41842191-41842210 MS.gene72959:CDS 30.0%
! TTGAAGAAAATGCGTTTTTC+TGG + chr8.3:41843000-41843019 MS.gene72959:CDS 30.0%
! TTTATTTGCTACAGAGAGTT+AGG + chr8.3:41841332-41841351 MS.gene72959:intron 30.0%
!! AGATATGACAAAACTTGCTA+AGG + chr8.3:41841774-41841793 MS.gene72959:intron 30.0%
!! AGCAAAGTCTTTGAAATGTT+CGG - chr8.3:41841020-41841039 None:intergenic 30.0%
!! AGTTTCAAAGTTTCTAAGAC+TGG - chr8.3:41842171-41842190 None:intergenic 30.0%
!! ATTGAGTTTAACTTGAGCTT+TGG + chr8.3:41842768-41842787 MS.gene72959:intron 30.0%
!! TGAAGTTATCAAGTTGAGAT+TGG - chr8.3:41841111-41841130 None:intergenic 30.0%
AAAATTTCGTATATGCCACG+GGG + chr8.3:41843072-41843091 MS.gene72959:CDS 35.0%
AAAGTGGATATAGGTCAAGT+GGG - chr8.3:41842914-41842933 None:intergenic 35.0%
AGAGATTCCTGCATCAATAA+TGG - chr8.3:41841520-41841539 None:intergenic 35.0%
ATCACCAATTACTACTCCAT+CGG - chr8.3:41841695-41841714 None:intergenic 35.0%
CATCGTAGAAATCAAAAGCT+TGG - chr8.3:41840970-41840989 None:intergenic 35.0%
CTACAGAGAGTTAGGTATAA+TGG + chr8.3:41841340-41841359 MS.gene72959:intron 35.0%
GAGATTCCTGCATCAATAAT+GGG - chr8.3:41841519-41841538 None:intergenic 35.0%
GATCTGTAAGTACGTTTGTT+GGG + chr8.3:41841549-41841568 MS.gene72959:intron 35.0%
GGTGAGATCTTTGTAGAATT+GGG + chr8.3:41842048-41842067 MS.gene72959:CDS 35.0%
TAAAGTGGATATAGGTCAAG+TGG - chr8.3:41842915-41842934 None:intergenic 35.0%
TATTGATGCAGGAATCTCTT+TGG + chr8.3:41841521-41841540 MS.gene72959:intron 35.0%
TCAAAACTCTTCAAGGAAGA+TGG - chr8.3:41842231-41842250 None:intergenic 35.0%
TGATCTGTAAGTACGTTTGT+TGG + chr8.3:41841548-41841567 MS.gene72959:intron 35.0%
TGTAGAATTGGGACAATCAT+CGG + chr8.3:41842059-41842078 MS.gene72959:CDS 35.0%
TTAGACATTCACTTGCAGAA+TGG + chr8.3:41841850-41841869 MS.gene72959:intron 35.0%
!! ACAACTAGACATTGGTAATG+TGG + chr8.3:41842110-41842129 MS.gene72959:CDS 35.0%
!! TTTAACTTGAGCTTTGGTGT+GGG + chr8.3:41842774-41842793 MS.gene72959:intron 35.0%
!!! GACTTTGCTATTTTCGTCAA+CGG + chr8.3:41841031-41841050 MS.gene72959:CDS 35.0%
!!! GCGTTTTTCTGGTTTTTTGT+TGG + chr8.3:41843011-41843030 MS.gene72959:CDS 35.0%
AAAAAGGTAACCTGATGGAG+AGG - chr8.3:41841264-41841283 None:intergenic 40.0%
AACAGATTCACTTCCAGAAG+AGG - chr8.3:41842400-41842419 None:intergenic 40.0%
AAGTGGGATGCAAGACAATT+AGG - chr8.3:41842898-41842917 None:intergenic 40.0%
ACAATGAAAGTTGACTGCGT+TGG + chr8.3:41841457-41841476 MS.gene72959:intron 40.0%
AGATGAAGATCCTCAAGGTA+CGG - chr8.3:41841175-41841194 None:intergenic 40.0%
AGCCTCACTGATATCACTAT+TGG + chr8.3:41842315-41842334 MS.gene72959:CDS 40.0%
CGGTGAGATCTTTGTAGAAT+TGG + chr8.3:41842047-41842066 MS.gene72959:CDS 40.0%
CTCATAGGTATCTTCATCTC+TGG + chr8.3:41840936-41840955 MS.gene72959:CDS 40.0%
GAAGATACCTATGAGAGACA+AGG - chr8.3:41840931-41840950 None:intergenic 40.0%
GATACAGCACCACATCAATT+TGG + chr8.3:41842847-41842866 MS.gene72959:CDS 40.0%
GGCAGAGAGAAGGATATTAA+TGG - chr8.3:41842145-41842164 None:intergenic 40.0%
GGCCAAAGATTCAGTTGAAA+AGG - chr8.3:41842196-41842215 None:intergenic 40.0%
GGTACGTACAAAACAACAAG+TGG - chr8.3:41842570-41842589 None:intergenic 40.0%
GTCCTAAGACCAAATTGATG+TGG - chr8.3:41842859-41842878 None:intergenic 40.0%
TAGTGATATCAGTGAGGCTA+AGG - chr8.3:41842314-41842333 None:intergenic 40.0%
TCAACTCCCATTATTGATGC+AGG + chr8.3:41841510-41841529 MS.gene72959:intron 40.0%
TGAATCTTTGGCCAAGCTTA+GGG + chr8.3:41842203-41842222 MS.gene72959:CDS 40.0%
TGTAATGCACACAGTCTCTA+CGG + chr8.3:41842027-41842046 MS.gene72959:intron 40.0%
! CACCACATCAATTTGGTCTT+AGG + chr8.3:41842854-41842873 MS.gene72959:CDS 40.0%
!! CAAAGTTTCTAAGACTGGAC+AGG - chr8.3:41842166-41842185 None:intergenic 40.0%
!! GTTTAACTTGAGCTTTGGTG+TGG + chr8.3:41842773-41842792 MS.gene72959:intron 40.0%
!! TACAGAAAAGCGTCGAAAGT+AGG - chr8.3:41842685-41842704 None:intergenic 40.0%
ACGGCTTTCTCTCTTTACGA+AGG + chr8.3:41841050-41841069 MS.gene72959:CDS 45.0%
AGAGGAGGTTAGTGCATGAT+AGG - chr8.3:41841246-41841265 None:intergenic 45.0%
CGCCAATAGTGATATCAGTG+AGG - chr8.3:41842320-41842339 None:intergenic 45.0%
CTGAATCTTTGGCCAAGCTT+AGG + chr8.3:41842202-41842221 MS.gene72959:CDS 45.0%
CTTGATGTAGTCTCCTCTTC+TGG + chr8.3:41842384-41842403 MS.gene72959:CDS 45.0%
CTTGGAGGAGAGTGAGTAAA+GGG - chr8.3:41842425-41842444 None:intergenic 45.0%
GGATCCGATGGAGTAGTAAT+TGG + chr8.3:41841688-41841707 MS.gene72959:intron 45.0%
GTAGAGTTTACTCACCAGCA+AGG + chr8.3:41841479-41841498 MS.gene72959:intron 45.0%
TGTCTTGAGATAGACACACC+TGG + chr8.3:41842279-41842298 MS.gene72959:CDS 45.0%
TTCTACGATGAGTACTCTCC+CGG + chr8.3:41840980-41840999 MS.gene72959:CDS 45.0%
! AGTTGTACCTTGTTTCCTGC+AGG + chr8.3:41842825-41842844 MS.gene72959:intron 45.0%
!! ATTTAACTCCAGAGAGCGCT+TGG - chr8.3:41842443-41842462 None:intergenic 45.0%
!! TAGAGGATGTTGGCTTGAGA+TGG + chr8.3:41842942-41842961 MS.gene72959:CDS 45.0%
!!! TTTTTTTTTTTTTTTTTGAA+AGG - chr8.3:41841437-41841456 None:intergenic 5.0%
AAGGTAACCTGATGGAGAGG+AGG - chr8.3:41841261-41841280 None:intergenic 50.0%
AGTTGATCTCACCACCTTGC+TGG - chr8.3:41841496-41841515 None:intergenic 50.0%
CGAGGAAAACTAGGATCCGA+TGG + chr8.3:41841676-41841695 MS.gene72959:intron 50.0%
CTCTTCTCGTCTGAGGATCA+GGG + chr8.3:41841199-41841218 MS.gene72959:CDS 50.0%
GAGTTTACTCACCAGCAAGG+TGG + chr8.3:41841482-41841501 MS.gene72959:intron 50.0%
GCTGTATCCTGCAGGAAACA+AGG - chr8.3:41842835-41842854 None:intergenic 50.0%
GCTTGGAGGAGAGTGAGTAA+AGG - chr8.3:41842426-41842445 None:intergenic 50.0%
GGAAGATGGAACCCTAAGCT+TGG - chr8.3:41842217-41842236 None:intergenic 50.0%
GGTCGAGATGAAGATCCTCA+AGG - chr8.3:41841180-41841199 None:intergenic 50.0%
GTGCAAGTTCCCAACAGAAG+TGG - chr8.3:41842346-41842365 None:intergenic 50.0%
TGTTGGGAACTTGCACAACG+TGG + chr8.3:41842350-41842369 MS.gene72959:CDS 50.0%
! AGGCTAAGGTACTTGAGACC+AGG - chr8.3:41842300-41842319 None:intergenic 50.0%
! ATTGGCGATGCCACTTCTGT+TGG + chr8.3:41842333-41842352 MS.gene72959:CDS 50.0%
! TTGGCGATGCCACTTCTGTT+GGG + chr8.3:41842334-41842353 MS.gene72959:CDS 50.0%
ACGCATCTTCCGAATGTCGC+GGG - chr8.3:41841077-41841096 None:intergenic 55.0%
AGACTGGACAGGCAGAGAGA+AGG - chr8.3:41842155-41842174 None:intergenic 55.0%
ATCCTCAAGGTACGGCCCAA+CGG - chr8.3:41841167-41841186 None:intergenic 55.0%
CCTCTTCTCGTCTGAGGATC+AGG + chr8.3:41841198-41841217 MS.gene72959:CDS 55.0%
CCTGATCCTCAGACGAGAAG+AGG - chr8.3:41841201-41841220 None:intergenic 55.0%
CGGAAGATGCGTCTCTCTTG+CGG + chr8.3:41841085-41841104 MS.gene72959:CDS 55.0%
CTGAACGCTCTGTTGACACG+TGG + chr8.3:41841131-41841150 MS.gene72959:CDS 55.0%
GACGCATCTTCCGAATGTCG+CGG - chr8.3:41841078-41841097 None:intergenic 55.0%
GCACTAACCTCCTCTCCATC+AGG + chr8.3:41841251-41841270 MS.gene72959:CDS 55.0%
TACGAAGGACCCGCGACATT+CGG + chr8.3:41841065-41841084 MS.gene72959:CDS 55.0%
TCTCGACCTCTTCTCGTCTG+AGG + chr8.3:41841192-41841211 MS.gene72959:CDS 55.0%
TGCATGATAGGACCGCGAGA+GGG - chr8.3:41841234-41841253 None:intergenic 55.0%
TGGGAACTTGCACAACGTGG+AGG + chr8.3:41842353-41842372 MS.gene72959:CDS 55.0%
!! GATGTGGTGCTGTATCCTGC+AGG - chr8.3:41842843-41842862 None:intergenic 55.0%
!! TAACTCCAGAGAGCGCTTGG+AGG - chr8.3:41842440-41842459 None:intergenic 55.0%
ACTCTCCTCCAAGCGCTCTC+TGG + chr8.3:41842432-41842451 MS.gene72959:CDS 60.0%
AGAGACCTGCCTTGTCGCTC+GGG + chr8.3:41841580-41841599 MS.gene72959:intron 60.0%
GAGAGACCTGCCTTGTCGCT+CGG + chr8.3:41841579-41841598 MS.gene72959:intron 60.0%
GGGCTTCAAACTCCCTCTCG+CGG + chr8.3:41841219-41841238 MS.gene72959:CDS 60.0%
GTGCATGATAGGACCGCGAG+AGG - chr8.3:41841235-41841254 None:intergenic 60.0%
AAGCTCCCGAGCGACAAGGC+AGG - chr8.3:41841588-41841607 None:intergenic 65.0%
ACGTGGATTCGCACCGCCGT+TGG + chr8.3:41841148-41841167 MS.gene72959:CDS 65.0%
CCAGAAGCTCCCGAGCGACA+AGG - chr8.3:41841592-41841611 None:intergenic 65.0%
CCTTGTCGCTCGGGAGCTTC+TGG + chr8.3:41841589-41841608 MS.gene72959:intron 65.0%
CGGCAGACAGTAGATCCCCG+TGG - chr8.3:41843090-41843109 None:intergenic 65.0%
CGTGGATTCGCACCGCCGTT+GGG + chr8.3:41841149-41841168 MS.gene72959:CDS 65.0%
CTCAAGGTACGGCCCAACGG+CGG - chr8.3:41841164-41841183 None:intergenic 65.0%
! GAGTACTCTCCCGGCGTCGA+CGG + chr8.3:41840989-41841008 MS.gene72959:CDS 65.0%
CGCCGTTGGGCCGTACCTTG+AGG + chr8.3:41841162-41841181 MS.gene72959:CDS 70.0%
TTCGGCCTCGCCGTCGACGC+CGG - chr8.3:41841002-41841021 None:intergenic 75.0%
! TCGGCCTCGCCGTCGACGCC+GGG - chr8.3:41841001-41841020 None:intergenic 80.0%
!!! CTCTCCCGGCGTCGACGGCG+AGG + chr8.3:41840994-41841013 MS.gene72959:CDS 80.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 41840923 41843125 41840923 ID=MS.gene72959
chr8.3 mRNA 41840923 41843125 41840923 ID=MS.gene72959.t1;Parent=MS.gene72959
chr8.3 exon 41840923 41841272 41840923 ID=MS.gene72959.t1.exon1;Parent=MS.gene72959.t1
chr8.3 CDS 41840923 41841272 41840923 ID=cds.MS.gene72959.t1;Parent=MS.gene72959.t1
chr8.3 exon 41842041 41842491 41842041 ID=MS.gene72959.t1.exon2;Parent=MS.gene72959.t1
chr8.3 CDS 41842041 41842491 41842041 ID=cds.MS.gene72959.t1;Parent=MS.gene72959.t1
chr8.3 exon 41842595 41842759 41842595 ID=MS.gene72959.t1.exon3;Parent=MS.gene72959.t1
chr8.3 CDS 41842595 41842759 41842595 ID=cds.MS.gene72959.t1;Parent=MS.gene72959.t1
chr8.3 exon 41842847 41843125 41842847 ID=MS.gene72959.t1.exon4;Parent=MS.gene72959.t1
chr8.3 CDS 41842847 41843125 41842847 ID=cds.MS.gene72959.t1;Parent=MS.gene72959.t1
Gene Sequence

>MS.gene72959

ATGAGCCTTGTCTCTCATAGGTATCTTCATCTCTGGAAATATCTCCAAGCTTTTGATTTCTACGATGAGTACTCTCCCGGCGTCGACGGCGAGGCCGAACATTTCAAAGACTTTGCTATTTTCGTCAACGGCTTTCTCTCTTTACGAAGGACCCGCGACATTCGGAAGATGCGTCTCTCTTGCGGCCAATCTCAACTTGATAACTTCACTGAACGCTCTGTTGACACGTGGATTCGCACCGCCGTTGGGCCGTACCTTGAGGATCTTCATCTCGACCTCTTCTCGTCTGAGGATCAGGGCTTCAAACTCCCTCTCGCGGTCCTATCATGCACTAACCTCCTCTCCATCAGGTTACCTTTTTTTTTTTTTTCCCTTCATATTTGAGTATTTTAGTCTCTCATTTTCAATGTTTATTTGCTACAGAGAGTTAGGTATAATGGGATAACAAATTATTAAAATTATTTTATGAAAAAGTAACAAGATTTATTGATTTTAACAATGAAAGTTGACTCCTTTCAAAAAAAAAAAAAAAAAACAATGAAAGTTGACTGCGTTGGTAGAGTTTACTCACCAGCAAGGTGGTGAGATCAACTCCCATTATTGATGCAGGAATCTCTTTGGTAGTTGATCTGTAAGTACGTTTGTTGGGTCTCACTGAGAGACCTGCCTTGTCGCTCGGGAGCTTCTGGAGCCCAACTCACCTAAACTTTCTAAAAAAAACATTTATTGATTGTTTGTTATATGAAAATAACTCGAGGAAAACTAGGATCCGATGGAGTAGTAATTGGTGATTTTATGATTATTATGCTAATTAAGCAAAGACATTTTCAATTAACAAACCAATTAATAAAAGATATGACAAAACTTGCTAAGGAAAAGACACTATGTCATGTTTTGTAGCATTGTTACATACATTCAACATTATTCTTAGACATTCACTTGCAGAATGGCTTTTTATTCTACTATTGTACTGGTATTTATTTACAAAAATTACAGTCTTTTTTAGAGCTTCAAAGATATTTCTTTCAGTCATTTCTTTATTTATTATTTGCACACTTGTTCTTGTTAAATGTGACATGATAAATGATGATAACATAAGATTTGTGTAATGCACACAGTCTCTACGGTGAGATCTTTGTAGAATTGGGACAATCATCGGCTGTTTGTTTACCATCATTGAAGAGATTACAACTAGACATTGGTAATGTGGATGTGAATGCCATTAATATCCTTCTCTCTGCCTGTCCAGTCTTAGAAACTTTGAAACTTTCCTTTTCAACTGAATCTTTGGCCAAGCTTAGGGTTCCATCTTCCTTGAAGAGTTTTGAATTTACTGTAGAGAATGAAATTGAAGTTTGTCTTGAGATAGACACACCTGGTCTCAAGTACCTTAGCCTCACTGATATCACTATTGGCGATGCCACTTCTGTTGGGAACTTGCACAACGTGGAGGAAGCATATCTTGATGTAGTCTCCTCTTCTGGAAGTGAATCTGTTGACCCTTTACTCACTCTCCTCCAAGCGCTCTCTGGAGTTAAATGTCTAGTGTTTAGTTGTAATACAACAATGGTGAGTGCAATTTTCGTTATCCATGATTATATATTCCTTTATATATTTAGTAGTCTTCTGCTAATTCTTGTATCTCCACTTGTTGTTTTGTACGTACCAAAAGAGATTATTAGGTAGTCAACGTATTATAGATTTTTCGAAAATGCACTTTTCGGAATTTCGCTGTTTACTACATCTAGAGCTCATTCTTCCTACTTTCGACGCTTTTCTGTACGTCGTGCTTCAGAAATGTCCTATACTTGAAGCTCTTATAATTCAGAATGACAAGGTTTGTGAATTGAGTTTAACTTGAGCTTTGGTGTGGGCGTGTGTTGTTTGTTTGATTATTTGGTGAGTTGTACCTTGTTTCCTGCAGGATACAGCACCACATCAATTTGGTCTTAGGACAAAGCCGAAAAGTGTTCCTAATTGTCTTGCATCCCACTTGACCTATATCCACTTTAAAGGATATAGAGGATGTTGGCTTGAGATGGACTTTGCTAGTTATGTTTTGCATTATGGACTTGTTTTGAAGAAAATGCGTTTTTCTGGTTTTTTGTTGGATGAAAGAAAGAAGTCGACAAAGTACCATTTTCTCAAAAAAATTTCGTATATGCCACGGGGATCTACTGTCTGCCGAGTTAAATATGACTAA
Protein sequence

>MS.gene72959.t1

MSLVSHRYLHLWKYLQAFDFYDEYSPGVDGEAEHFKDFAIFVNGFLSLRRTRDIRKMRLSCGQSQLDNFTERSVDTWIRTAVGPYLEDLHLDLFSSEDQGFKLPLAVLSCTNLLSISLYGEIFVELGQSSAVCLPSLKRLQLDIGNVDVNAINILLSACPVLETLKLSFSTESLAKLRVPSSLKSFEFTVENEIEVCLEIDTPGLKYLSLTDITIGDATSVGNLHNVEEAYLDVVSSSGSESVDPLLTLLQALSGVKCLVFSCNTTMRLLGSQRIIDFSKMHFSEFRCLLHLELILPTFDAFLYVVLQKCPILEALIIQNDKDTAPHQFGLRTKPKSVPNCLASHLTYIHFKGYRGCWLEMDFASYVLHYGLVLKKMRFSGFLLDERKKSTKYHFLKKISYMPRGSTVCRVKYD