AlfalfaGEDB | MS.gene72959

M. sativa cultivar XinJiangDaYe / MS.gene72959

KEGG: -
GO: -
PFAM: PF08387.13

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72959.t1	XP_013445781.1	79	415	84	2	1	414	51	463	1.80E-180	642.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72959.t1	Q8H1R7	27.6	424	264	14	2	414	28	419	8.9e-24	112.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72959.t1	A0A072TQY2	79.0	415	84	2	1	414	51	463	1.3e-180	642.1

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene03750	MS.gene72959	PPI
MS.gene03752	MS.gene72959	PPI
MS.gene055880	MS.gene72959	PPI
MS.gene055883	MS.gene72959	PPI
MS.gene065234	MS.gene72959	PPI
MS.gene065516	MS.gene72959	PPI
MS.gene072547	MS.gene72959	PPI
MS.gene072985	MS.gene72959	PPI
MS.gene09360	MS.gene72959	PPI
MS.gene09362	MS.gene72959	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72959.t1	MTR_8g468810	79.036	415	84	2	1	414	51	463	0.0	655
MS.gene72959.t1	MTR_8g468810	81.734	323	56	2	1	322	51	371	0.0	521
MS.gene72959.t1	MTR_8g058970	62.587	433	143	3	1	414	45	477	0.0	517
MS.gene72959.t1	MTR_8g468810	82.510	263	44	1	1	263	51	311	1.82e-151	432
MS.gene72959.t1	MTR_8g468810	82.510	263	44	1	1	263	51	311	3.15e-151	431
MS.gene72959.t1	MTR_8g468810	82.510	263	44	1	1	263	51	311	5.56e-151	432
MS.gene72959.t1	MTR_2g083840	54.306	418	180	6	1	414	47	457	1.47e-141	412
MS.gene72959.t1	MTR_8g062270	47.552	429	193	11	1	413	41	453	1.19e-115	345
MS.gene72959.t1	MTR_4g032730	47.045	440	188	9	3	413	43	466	2.47e-114	342
MS.gene72959.t1	MTR_5g092960	49.767	430	182	13	3	413	47	461	1.12e-109	330

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72959.t1	AT4G26340	28.337	427	257	16	2	414	28	419	1.41e-26	110
MS.gene72959.t1	AT1G78750	26.682	431	276	15	2	411	44	455	8.65e-23	100
MS.gene72959.t1	AT1G78750	26.682	431	276	15	2	411	44	455	8.65e-23	100
MS.gene72959.t1	AT5G38590	27.436	390	231	14	2	376	28	380	3.53e-20	92.4
MS.gene72959.t1	AT1G66300	28.409	440	242	21	3	411	56	453	6.72e-20	91.7
MS.gene72959.t1	AT4G13985	26.018	442	267	18	1	411	44	456	8.85e-20	91.7
MS.gene72959.t1	AT1G51370	27.315	432	248	23	2	411	45	432	2.01e-19	90.1
MS.gene72959.t1	AT5G56420	25.229	436	258	18	2	414	32	422	4.57e-19	89.0
MS.gene72959.t1	AT5G56420	25.229	436	258	18	2	414	32	422	4.57e-19	89.0
MS.gene72959.t1	AT5G56420	25.229	436	258	18	2	414	32	422	4.57e-19	89.0

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Find 88 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTTCGTATATGCCACG+GGG	0.751789	8.3:+41843072	MS.gene72959:CDS
AAAGTGGATATAGGTCAAGT+GGG	0.643217	8.3:-41842911	None:intergenic
AACAGATTCACTTCCAGAAG+AGG	0.569559	8.3:-41842397	None:intergenic
AAGAGATTACAACTAGACAT+TGG	0.609472	8.3:+41842102	MS.gene72959:CDS
AAGGTAACCTGATGGAGAGG+AGG	0.620939	8.3:-41841258	None:intergenic
AAGTGGGATGCAAGACAATT+AGG	0.332413	8.3:-41842895	None:intergenic
ACAACTAGACATTGGTAATG+TGG	0.620834	8.3:+41842110	MS.gene72959:CDS
ACGCATCTTCCGAATGTCGC+GGG	0.555891	8.3:-41841074	None:intergenic
ACGGCTTTCTCTCTTTACGA+AGG	0.645259	8.3:+41841050	MS.gene72959:CDS
ACGTACCAAAAGAGATTATT+AGG	0.292927	8.3:+41842583	MS.gene72959:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAATTTCGTATATGCCAC+GGG	+	chr8.3:41843071-41843090	MS.gene72959:CDS	30.0%
AAAATAACTCGAGGAAAACT+AGG	+	chr8.3:41841667-41841686	MS.gene72959:intron	30.0%
AAGAGATTACAACTAGACAT+TGG	+	chr8.3:41842102-41842121	MS.gene72959:CDS	30.0%
ACGTACCAAAAGAGATTATT+AGG	+	chr8.3:41842583-41842602	MS.gene72959:intron	30.0%
AGTTGTAATCTCTTCAATGA+TGG	-	chr8.3:41842096-41842115	None:intergenic	30.0%
TACAGAGAGTTAGGTATAAT+GGG	+	chr8.3:41841341-41841360	MS.gene72959:intron	30.0%
TTAAAGGATATAGAGGATGT+TGG	+	chr8.3:41842932-41842951	MS.gene72959:CDS	30.0%
TTAGAAAGTTTAGGTGAGTT+GGG	-	chr8.3:41841617-41841636	None:intergenic	30.0%
TTGACCTATATCCACTTTAA+AGG	+	chr8.3:41842916-41842935	MS.gene72959:CDS	30.0%
TTTAGAAAGTTTAGGTGAGT+TGG	-	chr8.3:41841618-41841637	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	41840923	41843125	41840923	ID=MS.gene72959
chr8.3	mRNA	41840923	41843125	41840923	ID=MS.gene72959.t1;Parent=MS.gene72959
chr8.3	exon	41840923	41841272	41840923	ID=MS.gene72959.t1.exon1;Parent=MS.gene72959.t1
chr8.3	CDS	41840923	41841272	41840923	ID=cds.MS.gene72959.t1;Parent=MS.gene72959.t1
chr8.3	exon	41842041	41842491	41842041	ID=MS.gene72959.t1.exon2;Parent=MS.gene72959.t1
chr8.3	CDS	41842041	41842491	41842041	ID=cds.MS.gene72959.t1;Parent=MS.gene72959.t1
chr8.3	exon	41842595	41842759	41842595	ID=MS.gene72959.t1.exon3;Parent=MS.gene72959.t1
chr8.3	CDS	41842595	41842759	41842595	ID=cds.MS.gene72959.t1;Parent=MS.gene72959.t1
chr8.3	exon	41842847	41843125	41842847	ID=MS.gene72959.t1.exon4;Parent=MS.gene72959.t1
chr8.3	CDS	41842847	41843125	41842847	ID=cds.MS.gene72959.t1;Parent=MS.gene72959.t1

Gene Sequence

>MS.gene72959

ATGAGCCTTGTCTCTCATAGGTATCTTCATCTCTGGAAATATCTCCAAGCTTTTGATTTCTACGATGAGTACTCTCCCGGCGTCGACGGCGAGGCCGAACATTTCAAAGACTTTGCTATTTTCGTCAACGGCTTTCTCTCTTTACGAAGGACCCGCGACATTCGGAAGATGCGTCTCTCTTGCGGCCAATCTCAACTTGATAACTTCACTGAACGCTCTGTTGACACGTGGATTCGCACCGCCGTTGGGCCGTACCTTGAGGATCTTCATCTCGACCTCTTCTCGTCTGAGGATCAGGGCTTCAAACTCCCTCTCGCGGTCCTATCATGCACTAACCTCCTCTCCATCAGGTTACCTTTTTTTTTTTTTTCCCTTCATATTTGAGTATTTTAGTCTCTCATTTTCAATGTTTATTTGCTACAGAGAGTTAGGTATAATGGGATAACAAATTATTAAAATTATTTTATGAAAAAGTAACAAGATTTATTGATTTTAACAATGAAAGTTGACTCCTTTCAAAAAAAAAAAAAAAAAACAATGAAAGTTGACTGCGTTGGTAGAGTTTACTCACCAGCAAGGTGGTGAGATCAACTCCCATTATTGATGCAGGAATCTCTTTGGTAGTTGATCTGTAAGTACGTTTGTTGGGTCTCACTGAGAGACCTGCCTTGTCGCTCGGGAGCTTCTGGAGCCCAACTCACCTAAACTTTCTAAAAAAAACATTTATTGATTGTTTGTTATATGAAAATAACTCGAGGAAAACTAGGATCCGATGGAGTAGTAATTGGTGATTTTATGATTATTATGCTAATTAAGCAAAGACATTTTCAATTAACAAACCAATTAATAAAAGATATGACAAAACTTGCTAAGGAAAAGACACTATGTCATGTTTTGTAGCATTGTTACATACATTCAACATTATTCTTAGACATTCACTTGCAGAATGGCTTTTTATTCTACTATTGTACTGGTATTTATTTACAAAAATTACAGTCTTTTTTAGAGCTTCAAAGATATTTCTTTCAGTCATTTCTTTATTTATTATTTGCACACTTGTTCTTGTTAAATGTGACATGATAAATGATGATAACATAAGATTTGTGTAATGCACACAGTCTCTACGGTGAGATCTTTGTAGAATTGGGACAATCATCGGCTGTTTGTTTACCATCATTGAAGAGATTACAACTAGACATTGGTAATGTGGATGTGAATGCCATTAATATCCTTCTCTCTGCCTGTCCAGTCTTAGAAACTTTGAAACTTTCCTTTTCAACTGAATCTTTGGCCAAGCTTAGGGTTCCATCTTCCTTGAAGAGTTTTGAATTTACTGTAGAGAATGAAATTGAAGTTTGTCTTGAGATAGACACACCTGGTCTCAAGTACCTTAGCCTCACTGATATCACTATTGGCGATGCCACTTCTGTTGGGAACTTGCACAACGTGGAGGAAGCATATCTTGATGTAGTCTCCTCTTCTGGAAGTGAATCTGTTGACCCTTTACTCACTCTCCTCCAAGCGCTCTCTGGAGTTAAATGTCTAGTGTTTAGTTGTAATACAACAATGGTGAGTGCAATTTTCGTTATCCATGATTATATATTCCTTTATATATTTAGTAGTCTTCTGCTAATTCTTGTATCTCCACTTGTTGTTTTGTACGTACCAAAAGAGATTATTAGGTAGTCAACGTATTATAGATTTTTCGAAAATGCACTTTTCGGAATTTCGCTGTTTACTACATCTAGAGCTCATTCTTCCTACTTTCGACGCTTTTCTGTACGTCGTGCTTCAGAAATGTCCTATACTTGAAGCTCTTATAATTCAGAATGACAAGGTTTGTGAATTGAGTTTAACTTGAGCTTTGGTGTGGGCGTGTGTTGTTTGTTTGATTATTTGGTGAGTTGTACCTTGTTTCCTGCAGGATACAGCACCACATCAATTTGGTCTTAGGACAAAGCCGAAAAGTGTTCCTAATTGTCTTGCATCCCACTTGACCTATATCCACTTTAAAGGATATAGAGGATGTTGGCTTGAGATGGACTTTGCTAGTTATGTTTTGCATTATGGACTTGTTTTGAAGAAAATGCGTTTTTCTGGTTTTTTGTTGGATGAAAGAAAGAAGTCGACAAAGTACCATTTTCTCAAAAAAATTTCGTATATGCCACGGGGATCTACTGTCTGCCGAGTTAAATATGACTAA

Protein sequence

>MS.gene72959.t1

MSLVSHRYLHLWKYLQAFDFYDEYSPGVDGEAEHFKDFAIFVNGFLSLRRTRDIRKMRLSCGQSQLDNFTERSVDTWIRTAVGPYLEDLHLDLFSSEDQGFKLPLAVLSCTNLLSISLYGEIFVELGQSSAVCLPSLKRLQLDIGNVDVNAINILLSACPVLETLKLSFSTESLAKLRVPSSLKSFEFTVENEIEVCLEIDTPGLKYLSLTDITIGDATSVGNLHNVEEAYLDVVSSSGSESVDPLLTLLQALSGVKCLVFSCNTTMRLLGSQRIIDFSKMHFSEFRCLLHLELILPTFDAFLYVVLQKCPILEALIIQNDKDTAPHQFGLRTKPKSVPNCLASHLTYIHFKGYRGCWLEMDFASYVLHYGLVLKKMRFSGFLLDERKKSTKYHFLKKISYMPRGSTVCRVKYD