AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32643

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32643.t1 MTR_3g107160 97.351 302 8 0 1 302 88 389 0.0 605
MS.gene32643.t1 MTR_3g107120 56.811 301 128 1 1 301 98 396 2.05e-113 333
MS.gene32643.t1 MTR_3g107100 52.667 300 125 3 14 296 99 398 5.37e-92 279
MS.gene32643.t1 MTR_3g106960 67.526 194 59 3 104 293 2 195 2.54e-81 246
MS.gene32643.t1 MTR_1g053025 41.720 314 154 10 1 295 85 388 2.19e-61 200
MS.gene32643.t1 MTR_2g007450 41.231 325 158 11 1 295 98 419 3.39e-60 197
MS.gene32643.t1 MTR_2g007400 38.291 316 171 10 1 297 94 404 1.32e-53 180
MS.gene32643.t1 MTR_1g107385 41.587 315 158 13 1 295 114 422 5.01e-52 176
MS.gene32643.t1 MTR_4g098590 42.446 278 146 7 14 280 92 366 2.19e-51 173
MS.gene32643.t1 MTR_7g083740 40.584 308 146 10 10 300 84 371 1.11e-50 171
MS.gene32643.t1 MTR_2g008320 39.615 260 142 6 48 297 174 428 2.11e-46 162
MS.gene32643.t1 MTR_7g075820 41.313 259 137 7 46 295 141 393 3.11e-46 160
MS.gene32643.t1 MTR_2g008310 40.154 259 140 7 48 297 120 372 1.19e-45 159
MS.gene32643.t1 MTR_2g007500 41.129 248 128 5 14 246 88 332 1.43e-45 157
MS.gene32643.t1 MTR_3g099830 40.530 264 136 8 46 295 117 373 2.77e-45 157
MS.gene32643.t1 MTR_2g005990 39.175 291 157 10 21 295 108 394 2.57e-44 155
MS.gene32643.t1 MTR_5g083890 40.714 280 138 10 17 274 106 379 2.83e-44 157
MS.gene32643.t1 MTR_7g007080 38.434 281 160 8 20 295 106 378 2.13e-43 153
MS.gene32643.t1 MTR_3g026920 34.146 328 159 12 1 296 103 405 8.17e-43 152
MS.gene32643.t1 MTR_2g007360 36.634 303 151 13 23 297 86 375 5.67e-42 149
MS.gene32643.t1 MTR_1g041245 33.910 289 172 8 21 296 104 386 1.47e-37 137
MS.gene32643.t1 MTR_7g056280 40.777 206 115 3 97 295 367 572 5.08e-37 139
MS.gene32643.t1 MTR_7g007340 40.239 251 126 10 50 288 111 349 6.32e-37 135
MS.gene32643.t1 MTR_6g029350 43.062 209 98 9 96 295 1 197 9.27e-37 131
MS.gene32643.t1 MTR_7g075800 33.333 312 156 12 4 268 88 394 8.72e-35 133
MS.gene32643.t1 MTR_3g094980 60.000 110 38 2 181 286 27 134 2.81e-34 123
MS.gene32643.t1 MTR_7g098510 31.724 290 179 7 20 296 108 391 4.19e-34 128
MS.gene32643.t1 MTR_2g007550 40.000 200 99 6 112 296 142 335 5.57e-34 127
MS.gene32643.t1 MTR_7g032370 31.379 290 167 11 20 296 82 352 5.08e-33 124
MS.gene32643.t1 MTR_7g098570 33.333 270 169 5 25 288 115 379 9.93e-33 126
MS.gene32643.t1 MTR_3g107140 50.820 122 58 1 1 122 78 197 1.58e-32 119
MS.gene32643.t1 MTR_3g108150 33.106 293 178 6 16 294 97 385 1.71e-32 124
MS.gene32643.t1 MTR_6g045333 32.237 304 156 11 20 293 160 443 2.91e-32 124
MS.gene32643.t1 MTR_6g022500 31.849 292 174 9 18 296 197 476 2.66e-31 122
MS.gene32643.t1 MTR_5g008950 32.374 278 169 7 28 296 98 365 5.84e-31 119
MS.gene32643.t1 MTR_1g108800 34.343 297 155 10 18 295 66 341 9.44e-31 118
MS.gene32643.t1 MTR_3g071850 32.095 296 165 10 17 289 120 402 1.35e-30 121
MS.gene32643.t1 MTR_7g098480 31.502 273 168 7 25 288 113 375 6.93e-30 118
MS.gene32643.t1 MTR_5g058100 30.872 298 179 10 16 298 101 386 9.66e-30 116
MS.gene32643.t1 MTR_1g066830 30.667 300 172 10 16 294 101 385 1.47e-29 116
MS.gene32643.t1 MTR_3g450920 50.704 142 54 5 1 138 88 217 4.38e-29 111
MS.gene32643.t1 MTR_7g032380 31.142 289 151 11 20 296 104 356 6.54e-29 114
MS.gene32643.t1 MTR_4g039440 34.164 281 156 12 17 283 123 388 1.14e-28 114
MS.gene32643.t1 MTR_5g058000 30.667 300 177 10 9 293 89 372 1.35e-28 113
MS.gene32643.t1 MTR_5g073420 31.058 293 178 9 12 301 101 372 1.75e-28 113
MS.gene32643.t1 MTR_7g026500 30.201 298 169 12 21 294 111 393 1.83e-28 113
MS.gene32643.t1 MTR_5g007410 33.333 273 150 9 14 270 120 376 2.66e-28 113
MS.gene32643.t1 MTR_1089s0010 32.997 297 159 12 21 298 106 381 3.35e-28 112
MS.gene32643.t1 MTR_3g036250 30.662 287 166 7 20 293 97 363 5.49e-28 111
MS.gene32643.t1 MTR_7g034115 32.867 286 168 11 21 292 104 379 1.51e-27 110
MS.gene32643.t1 MTR_7g115270 30.033 303 180 8 20 298 104 398 2.71e-27 110
MS.gene32643.t1 MTR_0543s0010 33.333 297 158 11 17 294 173 448 2.78e-27 110
MS.gene32643.t1 MTR_7g034090 41.954 174 91 4 135 301 147 317 3.05e-27 110
MS.gene32643.t1 MTR_7g034120 29.758 289 181 10 20 296 30 308 4.93e-27 107
MS.gene32643.t1 MTR_2g007090 35.000 260 130 11 42 293 63 291 5.28e-27 107
MS.gene32643.t1 MTR_6g040270 30.952 294 167 12 18 293 103 378 6.35e-27 108
MS.gene32643.t1 MTR_7g033990 32.867 286 162 13 20 288 103 375 7.68e-27 108
MS.gene32643.t1 MTR_5g045170 29.642 307 178 11 18 301 106 397 5.49e-26 106
MS.gene32643.t1 MTR_5g008920 30.104 289 183 8 17 296 103 381 6.31e-26 106
MS.gene32643.t1 MTR_7g056020 33.216 283 156 9 25 288 98 366 1.57e-25 106
MS.gene32643.t1 MTR_3g105060 27.797 295 185 9 11 296 98 373 1.90e-25 104
MS.gene32643.t1 MTR_2g036470 32.119 302 162 12 17 293 110 393 2.73e-25 104
MS.gene32643.t1 MTR_0492s0020 33.453 278 151 10 17 281 173 429 3.16e-25 104
MS.gene32643.t1 MTR_7g088260 30.872 298 176 11 7 288 172 455 9.74e-25 103
MS.gene32643.t1 MTR_6g011460 29.900 301 186 7 11 298 135 423 1.08e-23 100
MS.gene32643.t1 MTR_1g085120 30.798 263 161 7 17 269 187 438 1.58e-23 100
MS.gene32643.t1 MTR_2g007610 42.446 139 74 3 160 295 15 150 2.63e-23 94.4
MS.gene32643.t1 MTR_1g085120 30.798 263 161 7 17 269 262 513 3.32e-23 99.8
MS.gene32643.t1 MTR_5g045510 30.612 294 158 10 18 293 104 369 3.69e-23 98.2
MS.gene32643.t1 MTR_7g055943 28.571 308 191 8 9 298 127 423 4.95e-23 98.6
MS.gene32643.t1 MTR_5g012840 30.175 285 142 11 9 282 64 302 6.57e-23 96.7
MS.gene32643.t1 MTR_6g022520 30.897 301 176 9 17 298 28 315 6.84e-23 96.7
MS.gene32643.t1 MTR_7g056433 27.815 302 187 10 15 296 99 389 7.81e-23 97.4
MS.gene32643.t1 MTR_1g108820 33.871 248 124 12 45 272 128 355 1.24e-22 97.1
MS.gene32643.t1 MTR_5g039470 30.303 264 149 10 21 265 80 327 1.72e-22 95.9
MS.gene32643.t1 MTR_6g022360 30.075 266 168 8 17 274 125 380 2.04e-22 96.7
MS.gene32643.t1 MTR_7g007370 37.826 230 128 8 46 267 93 315 3.66e-22 95.1
MS.gene32643.t1 MTR_3g109982 29.961 257 156 6 47 291 220 464 7.61e-22 96.3
MS.gene32643.t1 MTR_3g082890 28.000 300 183 8 17 287 183 478 1.50e-21 94.7
MS.gene32643.t1 MTR_3g040700 29.572 257 151 7 16 262 131 367 1.53e-21 94.4
MS.gene32643.t1 MTR_0009s0080 30.370 270 162 8 14 269 110 367 2.90e-21 93.6
MS.gene32643.t1 MTR_5g039450 28.082 292 156 10 21 294 101 356 1.68e-20 90.5
MS.gene32643.t1 MTR_5g073340 31.004 229 148 3 17 238 75 300 2.20e-20 89.7
MS.gene32643.t1 MTR_3g108560 32.847 274 159 10 20 281 108 368 2.40e-20 90.5
MS.gene32643.t1 MTR_3g023630 28.464 267 170 8 13 269 90 345 3.37e-19 87.0
MS.gene32643.t1 MTR_5g054920 30.052 193 110 6 93 273 2 181 3.85e-19 84.0
MS.gene32643.t1 MTR_5g031970 27.778 288 167 9 18 293 95 353 4.92e-19 86.7
MS.gene32643.t1 MTR_7g056420 29.167 312 155 12 5 288 84 357 7.05e-19 87.4
MS.gene32643.t1 MTR_7g056430 29.167 312 155 12 5 288 84 357 7.40e-19 87.4
MS.gene32643.t1 MTR_5g073370 28.996 269 157 10 45 298 133 382 1.02e-18 85.9
MS.gene32643.t1 MTR_5g013500 30.738 244 147 8 73 296 124 365 1.18e-18 85.5
MS.gene32643.t1 MTR_3g087260 31.095 283 174 8 25 294 181 455 1.26e-18 86.3
MS.gene32643.t1 MTR_7g033915 32.110 218 116 10 92 293 161 362 1.64e-18 85.1
MS.gene32643.t1 MTR_6g044572 28.966 290 167 7 25 302 132 394 3.45e-18 84.3
MS.gene32643.t1 MTR_7g114710 27.273 308 162 11 11 296 97 364 3.86e-18 84.0
MS.gene32643.t1 MTR_5g063950 28.289 304 188 10 9 295 71 361 6.73e-18 83.2
MS.gene32643.t1 MTR_6g044560 28.966 290 167 7 25 302 308 570 1.81e-17 82.8
MS.gene32643.t1 MTR_2g007530 28.378 296 123 9 10 296 122 337 4.85e-17 80.5
MS.gene32643.t1 MTR_5g024060 37.500 136 73 4 159 288 71 200 6.04e-17 79.0
MS.gene32643.t1 MTR_7g056277 27.526 287 178 9 19 293 357 625 1.84e-16 80.1
MS.gene32643.t1 MTR_3g071790 29.189 185 111 6 92 263 25 202 2.27e-16 76.6
MS.gene32643.t1 MTR_7g060470 28.520 277 155 12 18 269 101 359 3.98e-16 78.6
MS.gene32643.t1 MTR_7g056287 27.208 283 184 7 19 293 101 369 1.13e-15 77.0
MS.gene32643.t1 MTR_4g080610 31.304 230 141 6 73 291 124 347 1.43e-15 76.6
MS.gene32643.t1 MTR_7g081290 34.921 126 68 3 167 282 120 241 1.99e-15 74.7
MS.gene32643.t1 MTR_7g021990 30.196 255 158 9 48 286 165 415 2.12e-15 76.6
MS.gene32643.t1 MTR_7g021950 30.196 255 158 9 48 286 165 415 2.17e-15 76.6
MS.gene32643.t1 MTR_3g467400 27.778 288 149 14 20 283 98 350 5.39e-15 75.1
MS.gene32643.t1 MTR_3g095630 32.237 152 87 6 123 269 11 151 5.39e-15 72.4
MS.gene32643.t1 MTR_1g059860 25.581 301 160 11 17 270 37 320 1.21e-14 73.6
MS.gene32643.t1 MTR_1377s0010 35.433 127 75 3 146 270 7 128 1.23e-14 70.1
MS.gene32643.t1 MTR_2g020180 30.345 145 75 4 164 296 85 215 1.83e-14 71.2
MS.gene32643.t1 MTR_3g115430 35.043 117 70 3 183 296 12 125 5.11e-14 68.2
MS.gene32643.t1 MTR_1g053050 48.718 78 39 1 219 295 37 114 5.56e-14 67.8
MS.gene32643.t1 MTR_1g043170 39.286 84 42 3 219 296 8 88 3.35e-13 64.7
MS.gene32643.t1 MTR_5g084070 28.417 278 141 8 47 272 126 397 4.20e-13 69.7
MS.gene32643.t1 MTR_5g039490 26.599 297 164 10 14 293 114 373 4.91e-13 69.3
MS.gene32643.t1 MTR_8g085430 37.324 142 70 8 144 272 164 299 5.51e-13 68.9
MS.gene32643.t1 MTR_5g076150 30.332 211 109 8 51 233 26 226 1.36e-12 66.6
MS.gene32643.t1 MTR_8g014160 24.658 292 193 7 20 296 146 425 7.69e-12 65.9
MS.gene32643.t1 MTR_7g022105 30.400 250 156 9 48 282 166 412 1.08e-11 65.5
MS.gene32643.t1 MTR_7g023310 32.432 111 72 2 170 280 331 438 3.57e-11 63.9
MS.gene32643.t1 MTR_5g092960 26.301 346 175 12 21 294 123 460 8.56e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32643.t1 AT4G26340 27.576 330 182 8 21 296 93 419 1.46e-21 94.4
MS.gene32643.t1 AT3G26920 31.365 271 157 10 45 294 37 299 2.27e-19 86.7
MS.gene32643.t1 AT4G10420 28.660 321 178 8 21 293 2 319 1.39e-17 82.0
MS.gene32643.t1 AT5G56380 26.957 345 177 12 21 296 101 439 7.99e-17 80.9
MS.gene32643.t1 AT5G56380 26.957 345 177 12 21 296 101 439 7.99e-17 80.9
MS.gene32643.t1 AT1G51370 24.650 357 190 12 1 295 95 434 1.34e-16 80.1
MS.gene32643.t1 AT5G56440 25.439 342 180 12 21 297 94 425 1.74e-16 79.7
MS.gene32643.t1 AT3G26920 29.276 304 159 13 45 294 37 338 3.18e-16 78.2
MS.gene32643.t1 AT3G26920 29.276 304 159 13 45 294 37 338 3.18e-16 78.2
MS.gene32643.t1 AT1G32375 24.629 337 199 9 14 296 87 422 9.36e-16 77.4
MS.gene32643.t1 AT1G32375 24.629 337 199 9 14 296 87 422 9.36e-16 77.4
MS.gene32643.t1 AT1G78750 24.011 354 177 10 20 295 118 457 1.38e-15 77.0
MS.gene32643.t1 AT1G78750 24.011 354 177 10 20 295 118 457 1.38e-15 77.0
MS.gene32643.t1 AT3G54910 28.000 325 187 10 17 296 51 373 1.74e-15 76.6
MS.gene32643.t1 AT3G54910 28.000 325 187 10 17 296 51 373 1.74e-15 76.6
MS.gene32643.t1 AT3G54910 28.000 325 187 10 17 296 51 373 1.74e-15 76.6
MS.gene32643.t1 AT5G56452 34.783 115 69 1 188 296 112 226 2.76e-14 71.2
MS.gene32643.t1 AT5G56452 34.783 115 69 1 188 296 113 227 2.80e-14 71.2
MS.gene32643.t1 AT5G44940 28.065 310 181 15 9 296 88 377 4.07e-14 72.4
MS.gene32643.t1 AT3G50710 25.826 333 190 11 21 296 95 427 2.62e-13 70.5
MS.gene32643.t1 AT5G56420 23.423 333 189 11 21 296 99 422 2.15e-12 67.4
MS.gene32643.t1 AT5G56420 23.423 333 189 11 21 296 99 422 2.15e-12 67.4
MS.gene32643.t1 AT5G56420 23.423 333 189 11 21 296 99 422 2.15e-12 67.4
MS.gene32643.t1 AT5G22610 24.540 326 190 11 21 293 111 433 2.66e-12 67.4
MS.gene32643.t1 AT1G51370 36.449 107 58 2 1 107 95 191 3.21e-12 67.0
MS.gene32643.t1 AT1G51370 36.449 107 58 2 1 107 95 191 5.20e-12 66.2
MS.gene32643.t1 AT1G16930 35.165 91 58 1 18 107 103 193 2.07e-11 64.7
MS.gene32643.t1 AT1G16930 35.165 91 58 1 18 107 103 193 2.07e-11 64.7
MS.gene32643.t1 AT1G16930 35.165 91 58 1 18 107 103 193 2.07e-11 64.7
MS.gene32643.t1 AT5G44850 28.772 285 174 13 20 288 52 323 4.27e-11 63.2

Find 55 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATAAAAGCTCACTTTCT+TGG 0.176566 3.1:+84705322 None:intergenic
TAGAAGGAATCGAAGAAAAT+TGG 0.191161 3.1:-84705416 MS.gene32643:CDS
AGACAATGAGGAACAGATAT+TGG 0.286231 3.1:+84705387 None:intergenic
CGATCACAACTTCACATATA+GGG 0.312073 3.1:+84705196 None:intergenic
TTGCAAGTGAGGATGCTAAT+AGG 0.327137 3.1:+84706106 None:intergenic
TAAGCTTTGTAGCTCTTGAT+AGG 0.357478 3.1:+84705886 None:intergenic
TCGATCACAACTTCACATAT+AGG 0.369694 3.1:+84705195 None:intergenic
TTTGTTCATAGAAATAAATA+AGG 0.381043 3.1:-84705783 MS.gene32643:intron
AGAGTTGCATTATGGACATT+TGG 0.382620 3.1:-84706258 None:intergenic
TCAAAATATTGAACTCAGTC+AGG 0.393649 3.1:-84705539 MS.gene32643:intron
GCTTTCGAAATTTCATCAAA+TGG 0.398360 3.1:-84706207 MS.gene32643:CDS
TCCTTCTATTATCTCATTGT+GGG 0.399087 3.1:+84705431 None:intergenic
AGAAGGAATCGAAGAAAATT+GGG 0.408150 3.1:-84705415 MS.gene32643:CDS
AGTTAGTTTCTTCAAAATGA+AGG 0.434643 3.1:+84706008 None:intergenic
GACAATGAGGAACAGATATT+GGG 0.437543 3.1:+84705388 None:intergenic
TTCCTTCTATTATCTCATTG+TGG 0.439702 3.1:+84705430 None:intergenic
ATGATCATCGTGCATGTTAG+TGG 0.443157 3.1:+84706141 None:intergenic
TGCAAGTGAGGATGCTAATA+GGG 0.443953 3.1:+84706107 None:intergenic
GCACGTAAATTCTCAAGATT+CGG 0.444469 3.1:+84705944 None:intergenic
AAACTCGAACTGGCATTTGT+TGG 0.455332 3.1:-84705590 MS.gene32643:CDS
TTCAAAATGAAGGATCTTGA+GGG 0.457781 3.1:+84706018 None:intergenic
CTTGAGGGAGGGGAGTGTAA+TGG 0.463433 3.1:+84706033 None:intergenic
TTTACTCTATAAACTCGAAC+TGG 0.468317 3.1:-84705600 MS.gene32643:CDS
CTTCAAAATGAAGGATCTTG+AGG 0.470897 3.1:+84706017 None:intergenic
GTTGTCAAATTGTGAAAGGT+AGG 0.479754 3.1:+84705631 None:intergenic
ACCCACAATGAGATAATAGA+AGG 0.480526 3.1:-84705432 MS.gene32643:CDS
GCATGTTAGTGGAAATTTCA+AGG 0.489092 3.1:+84706152 None:intergenic
GATTTAATATTACTTCTAGC+AGG 0.499995 3.1:-84705971 MS.gene32643:CDS
TTTCTATAAGTTCGTTGTGA+AGG 0.501551 3.1:-84706065 MS.gene32643:CDS
GAAATTTCGAAAGCCACGAC+GGG 0.503829 3.1:+84706216 None:intergenic
AATGAAGGATCTTGAGGGAG+GGG 0.517549 3.1:+84706023 None:intergenic
GAGAGTTATTATTATGCGCA+AGG 0.530407 3.1:-84705224 MS.gene32643:CDS
AATGATTATCTTTCCCGTCG+TGG 0.539229 3.1:-84706229 MS.gene32643:CDS
CATCAAATGGATTAATCATG+TGG 0.549064 3.1:-84706194 MS.gene32643:CDS
TGAAATTTCGAAAGCCACGA+CGG 0.554190 3.1:+84706215 None:intergenic
ACATTATGAAGTGCTTTCAG+TGG 0.554780 3.1:+84705812 None:intergenic
GAACAAGGGTCTTGCAAGTG+AGG 0.558852 3.1:+84706095 None:intergenic
GTTGTGATCGATTGCAAGCG+AGG 0.567231 3.1:-84705183 MS.gene32643:CDS
GAAGTGCTTTCAGTGGAAAG+TGG 0.575993 3.1:+84705819 None:intergenic
AAATGAAGGATCTTGAGGGA+GGG 0.584441 3.1:+84706022 None:intergenic
TTATTTGTTTAGGTGTATCG+AGG 0.588335 3.1:-84705667 MS.gene32643:intron
ATGCTAATAGGGAATTTGAA+AGG 0.596817 3.1:+84706118 None:intergenic
GATCACAACTTCACATATAG+GGG 0.597898 3.1:+84705197 None:intergenic
AGAGTTATTATTATGCGCAA+GGG 0.608669 3.1:-84705223 MS.gene32643:CDS
AGTTTATAGAGTAAAGTGCA+AGG 0.613124 3.1:+84705608 None:intergenic
GTTTATAGAGTAAAGTGCAA+GGG 0.614792 3.1:+84705609 None:intergenic
AAAATGAAGGATCTTGAGGG+AGG 0.631237 3.1:+84706021 None:intergenic
TATAGAAATCTAGATGAACA+AGG 0.632393 3.1:+84706080 None:intergenic
AAGGGTTGTCAAATTGTGAA+AGG 0.643954 3.1:+84705627 None:intergenic
CAAACCATGAAAATCAATTG+CGG 0.646623 3.1:-84705267 MS.gene32643:CDS
AGGAATCGAAGAAAATTGGG+AGG 0.653281 3.1:-84705412 MS.gene32643:CDS
ATAGAAATCTAGATGAACAA+GGG 0.662564 3.1:+84706081 None:intergenic
CACAACTTCACATATAGGGG+AGG 0.667240 3.1:+84705200 None:intergenic
AGGTAAGAGAGTCTTCAGAG+TGG 0.706824 3.1:+84705906 None:intergenic
ATTAATCATGTGGTGCAACG+TGG 0.709357 3.1:-84706184 MS.gene32643:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGTTCATAGAAATAAATA+AGG - chr3.1:84705637-84705656 MS.gene32643:CDS 15.0%
! AAGATATACCGTGAAAAATA+AGG + chr3.1:84705723-84705742 None:intergenic 25.0%
! ACCTAAACAAATAAACAACA+GGG + chr3.1:84705747-84705766 None:intergenic 25.0%
! ATAGAAATCTAGATGAACAA+GGG + chr3.1:84705342-84705361 None:intergenic 25.0%
! GATTTAATATTACTTCTAGC+AGG - chr3.1:84705449-84705468 MS.gene32643:intron 25.0%
! TATAGAAATCTAGATGAACA+AGG + chr3.1:84705343-84705362 None:intergenic 25.0%
!! AGTTAGTTTCTTCAAAATGA+AGG + chr3.1:84705415-84705434 None:intergenic 25.0%
!!! AATTTTGGCCTTATTTTTCA+CGG - chr3.1:84705712-84705731 MS.gene32643:intron 25.0%
!!! AGTTCAATATTTTGAAGGTT+AGG + chr3.1:84705876-84705895 None:intergenic 25.0%
!!! AGTTTTAATGAAAGACAATG+AGG + chr3.1:84706048-84706067 None:intergenic 25.0%
!!! ATAAATCAAAAAGGCATTCT+AGG + chr3.1:84705944-84705963 None:intergenic 25.0%
!!! GTTCAATATTTTGAAGGTTA+GGG + chr3.1:84705875-84705894 None:intergenic 25.0%
!!! TGCTCAATTTTGAAATTCTT+GGG - chr3.1:84706075-84706094 MS.gene32643:CDS 25.0%
!!! TTGATTTGCAGTTTTGTTTT+GGG - chr3.1:84705689-84705708 MS.gene32643:intron 25.0%
!!! TTGCTCAATTTTGAAATTCT+TGG - chr3.1:84706074-84706093 MS.gene32643:CDS 25.0%
AGAAGGAATCGAAGAAAATT+GGG - chr3.1:84706005-84706024 MS.gene32643:CDS 30.0%
AGAGTTATTATTATGCGCAA+GGG - chr3.1:84706197-84706216 MS.gene32643:CDS 30.0%
AGTTTATAGAGTAAAGTGCA+AGG + chr3.1:84705815-84705834 None:intergenic 30.0%
ATGCTAATAGGGAATTTGAA+AGG + chr3.1:84705305-84705324 None:intergenic 30.0%
CAAACCATGAAAATCAATTG+CGG - chr3.1:84706153-84706172 MS.gene32643:CDS 30.0%
CACCTAAACAAATAAACAAC+AGG + chr3.1:84705748-84705767 None:intergenic 30.0%
GCTTTCGAAATTTCATCAAA+TGG - chr3.1:84705213-84705232 MS.gene32643:CDS 30.0%
GTTTATAGAGTAAAGTGCAA+GGG + chr3.1:84705814-84705833 None:intergenic 30.0%
TAGAAGGAATCGAAGAAAAT+TGG - chr3.1:84706004-84706023 MS.gene32643:CDS 30.0%
TCAAAATATTGAACTCAGTC+AGG - chr3.1:84705881-84705900 MS.gene32643:CDS 30.0%
TCCTTCTATTATCTCATTGT+GGG + chr3.1:84705992-84706011 None:intergenic 30.0%
TTATTTGTTTAGGTGTATCG+AGG - chr3.1:84705753-84705772 MS.gene32643:intron 30.0%
TTCAAAATGAAGGATCTTGA+GGG + chr3.1:84705405-84705424 None:intergenic 30.0%
TTCCTTCTATTATCTCATTG+TGG + chr3.1:84705993-84706012 None:intergenic 30.0%
TTTACTCTATAAACTCGAAC+TGG - chr3.1:84705820-84705839 MS.gene32643:CDS 30.0%
TTTCTATAAGTTCGTTGTGA+AGG - chr3.1:84705355-84705374 MS.gene32643:CDS 30.0%
! TCCCTGTTGTTTATTTGTTT+AGG - chr3.1:84705743-84705762 MS.gene32643:intron 30.0%
!! CATCAAATGGATTAATCATG+TGG - chr3.1:84705226-84705245 MS.gene32643:CDS 30.0%
!! GACTGAGTTCAATATTTTGA+AGG + chr3.1:84705881-84705900 None:intergenic 30.0%
!! TTTACCGCAATTGATTTTCA+TGG + chr3.1:84706160-84706179 None:intergenic 30.0%
!!! CAGTTTTGTTTTGGGAATTT+TGG - chr3.1:84705697-84705716 MS.gene32643:intron 30.0%
!!! CTTGATTTGCAGTTTTGTTT+TGG - chr3.1:84705688-84705707 MS.gene32643:intron 30.0%
AAGGGTTGTCAAATTGTGAA+AGG + chr3.1:84705796-84705815 None:intergenic 35.0%
ACCCACAATGAGATAATAGA+AGG - chr3.1:84705988-84706007 MS.gene32643:CDS 35.0%
AGACAATGAGGAACAGATAT+TGG + chr3.1:84706036-84706055 None:intergenic 35.0%
AGTTGACGAAAGCAAAAATG+TGG - chr3.1:84705567-84705586 MS.gene32643:CDS 35.0%
CCAATAAAAGCTCACTTTCT+TGG + chr3.1:84706101-84706120 None:intergenic 35.0%
CGATCACAACTTCACATATA+GGG + chr3.1:84706227-84706246 None:intergenic 35.0%
CTTCAAAATGAAGGATCTTG+AGG + chr3.1:84705406-84705425 None:intergenic 35.0%
GACAATGAGGAACAGATATT+GGG + chr3.1:84706035-84706054 None:intergenic 35.0%
GAGAGTTATTATTATGCGCA+AGG - chr3.1:84706196-84706215 MS.gene32643:CDS 35.0%
GATCACAACTTCACATATAG+GGG + chr3.1:84706226-84706245 None:intergenic 35.0%
GCACGTAAATTCTCAAGATT+CGG + chr3.1:84705479-84705498 None:intergenic 35.0%
GCATGTTAGTGGAAATTTCA+AGG + chr3.1:84705271-84705290 None:intergenic 35.0%
GTTGACGAAAGCAAAAATGT+GGG - chr3.1:84705568-84705587 MS.gene32643:CDS 35.0%
GTTGTCAAATTGTGAAAGGT+AGG + chr3.1:84705792-84705811 None:intergenic 35.0%
TCGATCACAACTTCACATAT+AGG + chr3.1:84706228-84706247 None:intergenic 35.0%
TTGACGAAAGCAAAAATGTG+GGG - chr3.1:84705569-84705588 MS.gene32643:CDS 35.0%
! ACATTATGAAGTGCTTTCAG+TGG + chr3.1:84705611-84705630 None:intergenic 35.0%
! AGTCAGGTTTGTTTGGTATA+GGG - chr3.1:84705897-84705916 MS.gene32643:CDS 35.0%
! CCAAGAAAGTGAGCTTTTAT+TGG - chr3.1:84706098-84706117 MS.gene32643:CDS 35.0%
! TAAGCTTTGTAGCTCTTGAT+AGG + chr3.1:84705537-84705556 None:intergenic 35.0%
AAAATGAAGGATCTTGAGGG+AGG + chr3.1:84705402-84705421 None:intergenic 40.0%
AAACTCGAACTGGCATTTGT+TGG - chr3.1:84705830-84705849 MS.gene32643:CDS 40.0%
AAATGAAGGATCTTGAGGGA+GGG + chr3.1:84705401-84705420 None:intergenic 40.0%
AATGATTATCTTTCCCGTCG+TGG - chr3.1:84705191-84705210 MS.gene32643:CDS 40.0%
AGGAATCGAAGAAAATTGGG+AGG - chr3.1:84706008-84706027 MS.gene32643:CDS 40.0%
ATGATCATCGTGCATGTTAG+TGG + chr3.1:84705282-84705301 None:intergenic 40.0%
ATTAATCATGTGGTGCAACG+TGG - chr3.1:84705236-84705255 MS.gene32643:CDS 40.0%
TGAAATTTCGAAAGCCACGA+CGG + chr3.1:84705208-84705227 None:intergenic 40.0%
TGCAAGTGAGGATGCTAATA+GGG + chr3.1:84705316-84705335 None:intergenic 40.0%
TTGCAAGTGAGGATGCTAAT+AGG + chr3.1:84705317-84705336 None:intergenic 40.0%
! CAGTCAGGTTTGTTTGGTAT+AGG - chr3.1:84705896-84705915 MS.gene32643:CDS 40.0%
! TGAACTCAGTCAGGTTTGTT+TGG - chr3.1:84705890-84705909 MS.gene32643:CDS 40.0%
AATGAAGGATCTTGAGGGAG+GGG + chr3.1:84705400-84705419 None:intergenic 45.0%
AGGTAAGAGAGTCTTCAGAG+TGG + chr3.1:84705517-84705536 None:intergenic 45.0%
CACAACTTCACATATAGGGG+AGG + chr3.1:84706223-84706242 None:intergenic 45.0%
GAAATTTCGAAAGCCACGAC+GGG + chr3.1:84705207-84705226 None:intergenic 45.0%
! GAAGTGCTTTCAGTGGAAAG+TGG + chr3.1:84705604-84705623 None:intergenic 45.0%
!! AAAAATTAAATAAATCAAAA+AGG + chr3.1:84705953-84705972 None:intergenic 5.0%
!!! TTAATTTTTCTATATTATTA+GGG - chr3.1:84705964-84705983 MS.gene32643:CDS 5.0%
!!! TTTAATTTTTCTATATTATT+AGG - chr3.1:84705963-84705982 MS.gene32643:CDS 5.0%
GAACAAGGGTCTTGCAAGTG+AGG + chr3.1:84705328-84705347 None:intergenic 50.0%
GTTGTGATCGATTGCAAGCG+AGG - chr3.1:84706237-84706256 MS.gene32643:CDS 50.0%
! CTTGAGGGAGGGGAGTGTAA+TGG + chr3.1:84705390-84705409 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 84705173 84706269 84705173 ID=MS.gene32643
chr3.1 mRNA 84705173 84706269 84705173 ID=MS.gene32643.t1;Parent=MS.gene32643
chr3.1 exon 84705784 84706269 84705784 ID=MS.gene32643.t1.exon1;Parent=MS.gene32643.t1
chr3.1 CDS 84705784 84706269 84705784 ID=cds.MS.gene32643.t1;Parent=MS.gene32643.t1
chr3.1 exon 84705540 84705677 84705540 ID=MS.gene32643.t1.exon2;Parent=MS.gene32643.t1
chr3.1 CDS 84705540 84705677 84705540 ID=cds.MS.gene32643.t1;Parent=MS.gene32643.t1
chr3.1 exon 84705173 84705457 84705173 ID=MS.gene32643.t1.exon3;Parent=MS.gene32643.t1
chr3.1 CDS 84705173 84705457 84705173 ID=cds.MS.gene32643.t1;Parent=MS.gene32643.t1
Gene Sequence

>MS.gene32643

ATGGACATTTGGTATCATAATGATTATCTTTCCCGTCGTGGCTTTCGAAATTTCATCAAATGGATTAATCATGTGGTGCAACGTGGAGTTGAGCACCTTGAAATTTCCACTAACATGCACGATGATCATCCTTTCAAATTCCCTATTAGCATCCTCACTTGCAAGACCCTTGTTCATCTAGATTTCTATAAGTTCGTTGTGAAGGATTTTTCTTCCATTACACTCCCCTCCCTCAAGATCCTTCATTTTGAAGAAACTAACTTCTTAAATTATCAAGATTTAATATTACTTCTAGCAGGATGTCCGAATCTTGAGAATTTACGTGCAACTTTTCTAGAGTTCCACTCTGAAGACTCTCTTACCTATCAAGAGCTACAAAGCTTAAGCTTAAACAAGTTGACGAAAGCAAAAATGTGGGGCACTTATTGCCACTTTCCACTGAAAGCACTTCATAATGTGAAGCTTTTGTTCATAGAAATAAATAAGGTTTGAATTTTATTATGAACATGATGCTACTTGATTTGCAGTTTTGTTTTGGGAATTTTGGCCTTATTTTTCACGGTATATCTTTCCCTGTTGTTTATTTGTTTAGGTGTATCGAGGTTGTGATGAGATTCCTACCTTTCACAATTTGACAACCCTTGCACTTTACTCTATAAACTCGAACTGGCATTTGTTGGCGCAAGTGCTCAATCACTGCCCTAACCTTCAAAATATTGAACTCAGTCAGGTTTGTTTGGTATAGGGATCATATCTGATTTATCATTTCCTAGAATGCCTTTTTGATTTATTTAATTTTTCTATATTATTAGGGAACCCACAATGAGATAATAGAAGGAATCGAAGAAAATTGGGAGGACCCAATATCTGTTCCTCATTGTCTTTCATTAAAACTTCAAACTTGCTCAATTTTGAAATTCTTGGGCCAAGAAAGTGAGCTTTTATTGGCAAAATATATTCTAAAGAATGCAAGAGTTTTGCAAACCATGAAAATCAATTGCGGTAAAGAGCTCAAAACACATAGAGAGTTATTATTATGCGCAAGGGCCTCCCCTATATGTGAAGTTGTGATCGATTGCAAGCGAGGTTCTATGTAA
Protein sequence

>MS.gene32643.t1

MDIWYHNDYLSRRGFRNFIKWINHVVQRGVEHLEISTNMHDDHPFKFPISILTCKTLVHLDFYKFVVKDFSSITLPSLKILHFEETNFLNYQDLILLLAGCPNLENLRATFLEFHSEDSLTYQELQSLSLNKLTKAKMWGTYCHFPLKALHNVKLLFIEINKVYRGCDEIPTFHNLTTLALYSINSNWHLLAQVLNHCPNLQNIELSQGTHNEIIEGIEENWEDPISVPHCLSLKLQTCSILKFLGQESELLLAKYILKNARVLQTMKINCGKELKTHRELLLCARASPICEVVIDCKRGSM