AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63218

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63218.t1 MTR_8g468810 76.627 415 89 3 1 409 51 463 0.0 634
MS.gene63218.t1 MTR_8g468810 78.882 322 60 3 1 316 51 370 9.53e-178 500
MS.gene63218.t1 MTR_8g058970 60.508 433 147 4 1 409 45 477 8.96e-176 499
MS.gene63218.t1 MTR_8g468810 79.468 263 47 2 1 258 51 311 3.97e-145 416
MS.gene63218.t1 MTR_8g468810 79.468 263 47 2 1 258 51 311 5.71e-145 415
MS.gene63218.t1 MTR_8g468810 79.468 263 47 2 1 258 51 311 9.77e-145 416
MS.gene63218.t1 MTR_2g083840 52.153 418 184 7 1 409 47 457 1.32e-131 386
MS.gene63218.t1 MTR_8g062270 45.688 429 196 12 1 408 41 453 1.05e-107 325
MS.gene63218.t1 MTR_4g032730 44.545 440 194 10 3 408 43 466 1.09e-105 320
MS.gene63218.t1 MTR_5g092960 48.605 430 182 15 3 408 47 461 1.98e-103 314
MS.gene63218.t1 MTR_8g467800 46.838 427 189 13 2 409 46 453 4.04e-103 313
MS.gene63218.t1 MTR_8g468840 45.863 423 198 11 2 407 40 448 5.28e-102 310
MS.gene63218.t1 MTR_8g468760 46.604 427 167 12 1 408 34 418 8.21e-102 308
MS.gene63218.t1 MTR_8g014260 41.844 423 214 15 4 409 44 451 6.33e-90 279
MS.gene63218.t1 MTR_8g445780 42.507 367 182 10 4 359 41 389 1.51e-74 238
MS.gene63218.t1 MTR_5g092960 49.249 333 141 12 3 316 47 370 1.69e-73 235
MS.gene63218.t1 MTR_8g469020 36.574 432 236 12 2 409 34 451 3.38e-72 233
MS.gene63218.t1 MTR_8g058965 38.407 427 234 11 2 409 40 456 4.59e-71 231
MS.gene63218.t1 MTR_8g070950 37.037 432 211 15 1 409 41 434 8.11e-67 219
MS.gene63218.t1 MTR_8g467800 50.000 270 118 7 2 261 46 308 5.54e-65 211
MS.gene63218.t1 MTR_4g050250 39.633 381 191 10 1 351 47 418 7.17e-65 213
MS.gene63218.t1 MTR_8g062080 35.377 424 232 14 1 409 49 445 4.61e-60 201
MS.gene63218.t1 MTR_8g062010 35.024 414 246 11 1 409 35 430 7.14e-60 201
MS.gene63218.t1 MTR_4g024710 41.497 294 158 8 119 409 65 347 2.74e-55 186
MS.gene63218.t1 MTR_8g062310 33.894 416 224 14 4 409 54 428 1.09e-52 182
MS.gene63218.t1 MTR_8g062300 33.482 448 237 16 1 409 52 477 3.27e-51 179
MS.gene63218.t1 MTR_4g024745 42.066 271 143 8 125 392 25 284 3.60e-46 160
MS.gene63218.t1 MTR_8g014210 42.798 243 125 6 169 409 1 231 6.47e-45 155
MS.gene63218.t1 MTR_8g014160 36.861 274 156 8 2 261 67 337 6.06e-41 150
MS.gene63218.t1 MTR_8g077460 30.769 416 200 15 4 409 38 375 1.26e-40 148
MS.gene63218.t1 MTR_8g467790 32.698 367 180 13 1 349 174 491 7.72e-40 149
MS.gene63218.t1 MTR_5g021550 50.000 116 55 3 4 116 47 162 7.14e-28 108
MS.gene63218.t1 MTR_2g086020 28.467 411 196 12 1 409 1 315 1.24e-25 106
MS.gene63218.t1 MTR_8g014290 42.276 123 62 5 4 117 38 160 3.11e-24 98.2
MS.gene63218.t1 MTR_5g080710 27.488 422 264 16 2 408 59 453 4.12e-23 101
MS.gene63218.t1 MTR_5g084070 26.632 383 238 13 2 371 39 391 8.88e-18 85.1
MS.gene63218.t1 MTR_4g056040 27.792 403 242 16 13 383 65 450 1.30e-17 85.1
MS.gene63218.t1 MTR_4g113810 33.708 178 90 7 57 216 1 168 3.74e-17 79.0
MS.gene63218.t1 MTR_8g014220 38.017 121 58 4 46 156 47 160 6.66e-16 75.1
MS.gene63218.t1 MTR_4g056070 28.775 351 195 17 41 359 109 436 3.75e-15 77.4
MS.gene63218.t1 MTR_4g024720 33.813 139 69 6 4 121 45 181 3.50e-14 71.6
MS.gene63218.t1 MTR_4g056020 27.528 356 223 16 41 374 69 411 2.06e-12 68.9
MS.gene63218.t1 MTR_8g014230 48.000 75 35 2 325 399 25 95 2.81e-12 63.2
MS.gene63218.t1 MTR_4g056030 26.849 365 212 16 41 373 69 410 4.84e-12 67.8
MS.gene63218.t1 MTR_1g041245 30.769 169 95 6 2 161 37 192 1.49e-11 65.9
MS.gene63218.t1 MTR_1g041245 32.609 138 80 4 281 409 253 386 5.90e-11 63.9
MS.gene63218.t1 MTR_4g055580 26.027 365 209 15 44 373 199 537 5.59e-11 64.7
MS.gene63218.t1 MTR_1g038710 25.284 352 227 12 48 373 85 426 8.40e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63218.t1 AT4G26340 27.103 428 256 16 2 409 28 419 6.22e-23 100
MS.gene63218.t1 AT1G78750 26.498 434 268 16 2 406 44 455 8.38e-23 100
MS.gene63218.t1 AT1G78750 26.498 434 268 16 2 406 44 455 8.38e-23 100
MS.gene63218.t1 AT1G66290 26.897 435 249 22 2 406 55 450 1.15e-19 90.9
MS.gene63218.t1 AT1G78760 25.635 433 260 22 2 406 42 440 1.35e-18 87.8
MS.gene63218.t1 AT5G56420 24.709 429 264 16 2 409 32 422 1.42e-18 87.4
MS.gene63218.t1 AT5G56420 24.709 429 264 16 2 409 32 422 1.42e-18 87.4
MS.gene63218.t1 AT5G56420 24.709 429 264 16 2 409 32 422 1.42e-18 87.4
MS.gene63218.t1 AT5G56370 26.157 432 255 19 2 408 28 420 8.67e-18 85.1
MS.gene63218.t1 AT5G56370 26.157 432 255 19 2 408 28 420 8.67e-18 85.1
MS.gene63218.t1 AT4G13985 23.928 443 270 15 1 406 44 456 1.01e-17 85.1
MS.gene63218.t1 AT1G69630 25.346 434 266 20 2 406 44 448 1.43e-17 84.7
MS.gene63218.t1 AT5G22730 27.488 422 239 18 26 406 68 463 6.30e-17 82.8
MS.gene63218.t1 AT5G22730 27.488 422 239 18 26 406 68 463 6.30e-17 82.8
MS.gene63218.t1 AT5G38570 31.273 275 156 11 142 408 126 375 2.09e-16 80.9
MS.gene63218.t1 AT1G51370 27.014 422 241 22 12 406 51 432 2.63e-16 80.9
MS.gene63218.t1 AT1G66300 26.712 438 247 21 3 406 56 453 2.81e-16 80.9
MS.gene63218.t1 AT1G32375 24.651 430 265 16 3 409 29 422 3.31e-15 77.4
MS.gene63218.t1 AT1G32375 24.651 430 265 16 3 409 29 422 3.31e-15 77.4
MS.gene63218.t1 AT5G22720 26.058 449 257 23 2 408 49 464 3.60e-15 77.4
MS.gene63218.t1 AT3G50710 25.290 431 266 18 3 409 29 427 4.21e-15 77.0
MS.gene63218.t1 AT5G62970 28.775 351 219 15 69 405 112 445 7.19e-15 76.6
MS.gene63218.t1 AT1G06630 26.496 351 197 14 2 321 47 367 1.10e-14 75.9
MS.gene63218.t1 AT1G06630 26.496 351 197 14 2 321 38 358 1.20e-14 75.5
MS.gene63218.t1 AT1G06630 26.496 351 197 14 2 321 38 358 1.20e-14 75.5
MS.gene63218.t1 AT1G06630 26.496 351 197 14 2 321 38 358 1.20e-14 75.5
MS.gene63218.t1 AT1G06630 26.496 351 197 14 2 321 38 358 1.20e-14 75.5
MS.gene63218.t1 AT5G22670 29.620 368 215 19 30 371 58 407 1.21e-14 75.9
MS.gene63218.t1 AT1G06630 26.453 344 193 13 2 314 38 352 1.28e-14 75.1
MS.gene63218.t1 AT1G06630 26.453 344 193 13 2 314 38 352 1.28e-14 75.1
MS.gene63218.t1 AT1G06630 26.496 351 197 14 2 321 38 358 1.34e-14 75.9
MS.gene63218.t1 AT3G54910 26.396 394 243 15 31 409 12 373 3.06e-14 73.9
MS.gene63218.t1 AT3G54910 26.396 394 243 15 31 409 12 373 3.06e-14 73.9
MS.gene63218.t1 AT3G54910 26.396 394 243 15 31 409 12 373 3.06e-14 73.9
MS.gene63218.t1 AT1G06630 28.261 276 170 10 2 261 38 301 3.77e-14 73.2
MS.gene63218.t1 AT4G10400 23.810 420 268 16 2 408 28 408 4.29e-14 73.9
MS.gene63218.t1 AT4G10400 23.810 420 268 16 2 408 28 408 4.29e-14 73.9
MS.gene63218.t1 AT4G10400 23.810 420 268 16 2 408 28 408 4.29e-14 73.9
MS.gene63218.t1 AT5G60610 25.500 400 232 15 2 375 28 387 5.30e-14 73.6
MS.gene63218.t1 AT5G60610 25.500 400 232 15 2 375 28 387 5.30e-14 73.6
MS.gene63218.t1 AT2G26030 26.818 440 261 21 2 406 30 443 5.80e-14 73.6
MS.gene63218.t1 AT5G44950 27.318 399 243 19 2 375 30 406 8.61e-14 73.2
MS.gene63218.t1 AT1G80470 23.721 430 267 20 2 406 41 434 8.90e-14 73.2
MS.gene63218.t1 AT3G49030 24.000 425 248 16 2 408 47 414 4.29e-13 70.9
MS.gene63218.t1 AT5G56700 26.400 375 228 17 14 370 2 346 1.50e-12 68.9
MS.gene63218.t1 AT1G55660 23.653 427 288 16 2 406 79 489 2.96e-12 68.6
MS.gene63218.t1 AT1G13780 26.812 414 237 21 2 375 36 423 7.63e-12 67.0
MS.gene63218.t1 AT2G26030 28.492 358 215 17 77 406 21 365 1.12e-11 66.2
MS.gene63218.t1 AT4G14096 26.966 356 213 13 12 339 40 376 1.34e-11 66.6
MS.gene63218.t1 AT4G14096 26.966 356 213 13 12 339 40 376 1.34e-11 66.6
MS.gene63218.t1 AT5G56810 28.716 296 174 10 125 406 159 431 2.89e-11 65.5
MS.gene63218.t1 AT3G58860 23.601 411 273 14 2 392 33 422 5.40e-11 64.7

Find 86 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGACCTATATCCACTTTAA+AGG 0.185801 8.4:+41606782 MS.gene63218:CDS
ACGTACCAAAAGAGATTATT+AGG 0.292927 8.4:+41606449 MS.gene63218:intron
CTTGATGTAGTCTCCTCTTC+TGG 0.309388 8.4:+41606250 MS.gene63218:CDS
AGAGGAGGTTAGTGCATGAT+AGG 0.328779 8.4:-41605187 None:intergenic
CACCACATCAATTTGGTCTT+AGG 0.331226 8.4:+41606720 MS.gene63218:CDS
AAGTGGGATGCAAGACAATT+AGG 0.332413 8.4:-41606761 None:intergenic
CTCCTCTCCTTCGCTGAAAT+AGG 0.369885 8.4:+41605203 MS.gene63218:CDS
CTGAATCTTTGGCCAAGCTT+AGG 0.379532 8.4:+41606068 MS.gene63218:CDS
GGCAGAGAGAAGGATATTAA+TGG 0.390004 8.4:-41606008 None:intergenic
AGCAAAGTCTTTGAAATGTT+CGG 0.401003 8.4:-41604961 None:intergenic
AGTAAATTCAAAACTCTTCA+AGG 0.409402 8.4:-41606101 None:intergenic
TACGACATACCCGCGACATT+CGG 0.412011 8.4:+41605009 MS.gene63218:CDS
ACTCTCCTCCAAGCGCTCTC+TGG 0.418872 8.4:+41606298 MS.gene63218:CDS
TTCGGCCTCGCCGTCGACGC+CGG 0.419368 8.4:-41604943 None:intergenic
TGAATCTTTGGCCAAGCTTA+GGG 0.420723 8.4:+41606069 MS.gene63218:CDS
CGAAGTTATCAAGTTGAGAT+TGG 0.421124 8.4:-41605052 None:intergenic
GATACAGCACCACATCAATT+TGG 0.425006 8.4:+41606713 MS.gene63218:CDS
AGCCTCACTGATATCACTAT+TGG 0.428235 8.4:+41606181 MS.gene63218:CDS
TCGGCCTCGCCGTCGACGCC+GGG 0.429312 8.4:-41604942 None:intergenic
GGCCAAAGATTCAGTTGAAA+AGG 0.438596 8.4:-41606059 None:intergenic
AGCTTCAAAGTTTCTAAGAC+TGG 0.444008 8.4:-41606034 None:intergenic
CTCATAGGTATCTTCATCTC+TGG 0.445374 8.4:+41604880 MS.gene63218:CDS
CGTGGATCCGCACTGCCGTT+GGG 0.447263 8.4:+41605093 MS.gene63218:CDS
ATTATAAGAGCTTCAAGTAT+AGG 0.447586 8.4:-41606590 None:intergenic
ATTTAACTCCAGAGAGCGCT+TGG 0.458373 8.4:-41606306 None:intergenic
CAATGAGCCTTGTTGCTCAT+AGG 0.458384 8.4:+41604865 None:intergenic
TTGGCGATGCCACTTCTGTT+GGG 0.460446 8.4:+41606200 MS.gene63218:CDS
ATCCTCTATATCCTTTAAAG+TGG 0.461419 8.4:-41606793 None:intergenic
ATCCACTTTAAAGGATATAG+AGG 0.462211 8.4:+41606791 MS.gene63218:CDS
CTCTTCTCGTCTGAGGATCA+AGG 0.463320 8.4:+41605143 MS.gene63218:CDS
TCAAAACTCTTCAAGGAAGA+TGG 0.465651 8.4:-41606094 None:intergenic
CTTGGAGGAGAGTGAGTAAA+GGG 0.475202 8.4:-41606288 None:intergenic
ATTGGCGATGCCACTTCTGT+TGG 0.491458 8.4:+41606199 MS.gene63218:CDS
TGTAGAATTGGGACAATCAT+CGG 0.493103 8.4:+41605925 MS.gene63218:intron
TTCTACAATGTGTACTCTCC+CGG 0.497786 8.4:+41604924 MS.gene63218:CDS
ATATCCTTTAAAGTGGATAT+AGG 0.498926 8.4:-41606786 None:intergenic
TTAAAGGATATAGAGGATGT+TGG 0.507552 8.4:+41606798 MS.gene63218:CDS
ATCCTCAAGGTGGGGCCCAA+CGG 0.507882 8.4:-41605108 None:intergenic
CAAAGTTTCTAAGACTGGAC+AGG 0.509020 8.4:-41606029 None:intergenic
ACGTGGATCCGCACTGCCGT+TGG 0.511251 8.4:+41605092 MS.gene63218:CDS
TAGAGGATGTTGGCTTGAGA+TGG 0.521759 8.4:+41606808 MS.gene63218:CDS
CATTGTAGAAATGAAAAGCT+TGG 0.524329 8.4:-41604911 None:intergenic
AGTTGTAATCTCTTCAATGA+TGG 0.525654 8.4:-41605959 None:intergenic
TAGTGATATCAGTGAGGCTA+AGG 0.528202 8.4:-41606177 None:intergenic
TACAGAAAAGCGTCGAAAGT+AGG 0.534200 8.4:-41606548 None:intergenic
TGCCGTTGGGCCCCACCTTG+AGG 0.539334 8.4:+41605106 MS.gene63218:CDS
GTGTACTCTCCCGGCGTCGA+CGG 0.554410 8.4:+41604933 MS.gene63218:CDS
ACGCATCTTCCGAATGTCGC+GGG 0.562648 8.4:-41605018 None:intergenic
CATTATACCTATTTCAGCGA+AGG 0.562844 8.4:-41605210 None:intergenic
TCTCGCTCTCTTCTCGTCTG+AGG 0.568024 8.4:+41605136 MS.gene63218:CDS
AACAGATTCACTTCCAGAAG+AGG 0.569559 8.4:-41606263 None:intergenic
TAACTCCAGAGAGCGCTTGG+AGG 0.571531 8.4:-41606303 None:intergenic
GCTTGGAGGAGAGTGAGTAA+AGG 0.575040 8.4:-41606289 None:intergenic
TAAAGTGGATATAGGTCAAG+TGG 0.575798 8.4:-41606778 None:intergenic
GGAAGATGGAACCCTAAGCT+TGG 0.579963 8.4:-41606080 None:intergenic
GTGCAAGTTCCCAACAGAAG+TGG 0.580230 8.4:-41606209 None:intergenic
AGACTGGACAGGCAGAGAGA+AGG 0.581233 8.4:-41606018 None:intergenic
GAGGTTAGTGCATGATAGGA+TGG 0.583085 8.4:-41605183 None:intergenic
TACCTATTTCAGCGAAGGAG+AGG 0.584479 8.4:-41605205 None:intergenic
CTGAACGCTCTGTTGACACG+TGG 0.601980 8.4:+41605075 MS.gene63218:CDS
GAGATGAAGATCCTCAAGGT+GGG 0.607880 8.4:-41605117 None:intergenic
AAGAGATTACAACTAGACAT+TGG 0.609472 8.4:+41605968 MS.gene63218:CDS
CGGAAGATGCGTCTCTCTTG+CGG 0.610368 8.4:+41605029 MS.gene63218:CDS
CTATTTCAGCGAAGGAGAGG+AGG 0.611882 8.4:-41605202 None:intergenic
GAACTTAGTCATATTTAACT+CGG 0.618369 8.4:-41606973 None:intergenic
ACAACTAGACATTGGTAATG+TGG 0.620834 8.4:+41605976 MS.gene63218:CDS
AGGCTAAGGTACTTGAGACC+AGG 0.622331 8.4:-41606163 None:intergenic
CTCTCCCGGCGTCGACGGCG+AGG 0.630012 8.4:+41604938 MS.gene63218:CDS
AGATGAAGATCCTCAAGGTG+GGG 0.631714 8.4:-41605116 None:intergenic
AAAGTGGATATAGGTCAAGT+GGG 0.643217 8.4:-41606777 None:intergenic
GACGCATCTTCCGAATGTCG+CGG 0.645697 8.4:-41605019 None:intergenic
CGAGATGAAGATCCTCAAGG+TGG 0.647298 8.4:-41605118 None:intergenic
GTTTAGTTGTAATACAACAA+TGG 0.648312 8.4:+41606336 MS.gene63218:CDS
GAGCGAGATGAAGATCCTCA+AGG 0.648810 8.4:-41605121 None:intergenic
TGCATGATAGGATGGCGAGA+GGG 0.650254 8.4:-41605175 None:intergenic
TGTTGGGAACTTGCACAACG+TGG 0.655593 8.4:+41606216 MS.gene63218:CDS
GTCCTAAGACCAAATTGATG+TGG 0.669559 8.4:-41606722 None:intergenic
TCTTATAATTCAGAATGACA+TGG 0.670895 8.4:+41606604 MS.gene63218:CDS
GGTGGGGCCCAACGGCAGTG+CGG 0.675625 8.4:-41605100 None:intergenic
TGTCTTGAGATAGACACACC+TGG 0.677521 8.4:+41606145 MS.gene63218:CDS
GTGCATGATAGGATGGCGAG+AGG 0.679069 8.4:-41605176 None:intergenic
GAAGATACCTATGAGCAACA+AGG 0.701705 8.4:-41604872 None:intergenic
TGGGAACTTGCACAACGTGG+AGG 0.706891 8.4:+41606219 MS.gene63218:CDS
CGCCAATAGTGATATCAGTG+AGG 0.714173 8.4:-41606183 None:intergenic
CGGCAGACAGTAGATCCCCG+TGG 0.745934 8.4:-41606953 None:intergenic
AAAATTTCGTATATGCCACG+GGG 0.751789 8.4:+41606938 MS.gene63218:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTTTTTTTTTTTTTTGAA+AGG - chr8.4:41605308-41605327 None:intergenic 10.0%
!! TATAAAGGAATATATAATCA+TGG - chr8.4:41606381-41606400 None:intergenic 15.0%
!!! TTGTCATATCTTTTATTAAT+TGG - chr8.4:41605631-41605650 None:intergenic 15.0%
!! GAAGACTACTAAATATATAA+AGG - chr8.4:41606396-41606415 None:intergenic 20.0%
!!! AATGTTTTTTTAGAAAGTTG+AGG - chr8.4:41605493-41605512 None:intergenic 20.0%
!!! ACGAAATTTTTTTGAGAAAA+TGG - chr8.4:41606928-41606947 None:intergenic 20.0%
!!! TTTTTATTCTACTATTGTAC+TGG + chr8.4:41605740-41605759 MS.gene63218:intron 20.0%
! AAAAAATTTCGTATATGCCA+CGG + chr8.4:41606936-41606955 MS.gene63218:CDS 25.0%
! AGTAAATTCAAAACTCTTCA+AGG - chr8.4:41606104-41606123 None:intergenic 25.0%
! ATTATAAGAGCTTCAAGTAT+AGG - chr8.4:41606593-41606612 None:intergenic 25.0%
! GTTTAGTTGTAATACAACAA+TGG + chr8.4:41606336-41606355 MS.gene63218:CDS 25.0%
! TCTTATAATTCAGAATGACA+TGG + chr8.4:41606604-41606623 MS.gene63218:CDS 25.0%
! TGTTATATGAAAATAACTCG+AGG + chr8.4:41605524-41605543 MS.gene63218:intron 25.0%
!!! ATATCCTTTAAAGTGGATAT+AGG - chr8.4:41606789-41606808 None:intergenic 25.0%
!!! GTGTTGTTTGTTTGATTATT+TGG + chr8.4:41606666-41606685 MS.gene63218:intron 25.0%
!!! TTTTTCGAAAATGCACTTTT+CGG + chr8.4:41606490-41606509 MS.gene63218:CDS 25.0%
AAAAATTTCGTATATGCCAC+GGG + chr8.4:41606937-41606956 MS.gene63218:CDS 30.0%
AAAATAACTCGAGGAAAACT+AGG + chr8.4:41605533-41605552 MS.gene63218:intron 30.0%
AAGAGATTACAACTAGACAT+TGG + chr8.4:41605968-41605987 MS.gene63218:CDS 30.0%
ACGTACCAAAAGAGATTATT+AGG + chr8.4:41606449-41606468 MS.gene63218:intron 30.0%
AGTTGTAATCTCTTCAATGA+TGG - chr8.4:41605962-41605981 None:intergenic 30.0%
CATTGTAGAAATGAAAAGCT+TGG - chr8.4:41604914-41604933 None:intergenic 30.0%
TTAAAGGATATAGAGGATGT+TGG + chr8.4:41606798-41606817 MS.gene63218:CDS 30.0%
TTCGCTGAAATAGGTATAAT+GGG + chr8.4:41605212-41605231 MS.gene63218:intron 30.0%
TTGACCTATATCCACTTTAA+AGG + chr8.4:41606782-41606801 MS.gene63218:CDS 30.0%
! AGACAATTAGGAACACTTTT+CGG - chr8.4:41606752-41606771 None:intergenic 30.0%
! ATCCACTTTAAAGGATATAG+AGG + chr8.4:41606791-41606810 MS.gene63218:CDS 30.0%
! ATCCTCTATATCCTTTAAAG+TGG - chr8.4:41606796-41606815 None:intergenic 30.0%
! GACTACCTAATAATCTCTTT+TGG - chr8.4:41606457-41606476 None:intergenic 30.0%
! GCTAGTTATGTTTTGCATTA+TGG + chr8.4:41606836-41606855 MS.gene63218:CDS 30.0%
! TTCCTTTTCAACTGAATCTT+TGG + chr8.4:41606057-41606076 MS.gene63218:CDS 30.0%
! TTGAAGAAAATGCGTTTTTC+TGG + chr8.4:41606866-41606885 MS.gene63218:CDS 30.0%
!! AGATATGACAAAACTTGCTA+AGG + chr8.4:41605640-41605659 MS.gene63218:intron 30.0%
!! AGCAAAGTCTTTGAAATGTT+CGG - chr8.4:41604964-41604983 None:intergenic 30.0%
!! ATTGAGTTTAACTTGAGCTT+TGG + chr8.4:41606634-41606653 MS.gene63218:intron 30.0%
AAAATTTCGTATATGCCACG+GGG + chr8.4:41606938-41606957 MS.gene63218:CDS 35.0%
AAAGTGGATATAGGTCAAGT+GGG - chr8.4:41606780-41606799 None:intergenic 35.0%
AGAGATTCCTGCATCAATAA+TGG - chr8.4:41605387-41605406 None:intergenic 35.0%
CATTATACCTATTTCAGCGA+AGG - chr8.4:41605213-41605232 None:intergenic 35.0%
CTTCGCTGAAATAGGTATAA+TGG + chr8.4:41605211-41605230 MS.gene63218:intron 35.0%
GAGATTCCTGCATCAATAAT+GGG - chr8.4:41605386-41605405 None:intergenic 35.0%
GATCTGTAAGTACGTTTGTT+GGG + chr8.4:41605416-41605435 MS.gene63218:intron 35.0%
GGATCTGATGGAGTAATAAT+TGG + chr8.4:41605554-41605573 MS.gene63218:intron 35.0%
GGTGAGATCTTTGTAGAATT+GGG + chr8.4:41605914-41605933 MS.gene63218:intron 35.0%
TAAAGTGGATATAGGTCAAG+TGG - chr8.4:41606781-41606800 None:intergenic 35.0%
TATTGATGCAGGAATCTCTT+TGG + chr8.4:41605388-41605407 MS.gene63218:intron 35.0%
TCAAAACTCTTCAAGGAAGA+TGG - chr8.4:41606097-41606116 None:intergenic 35.0%
TGATCTGTAAGTACGTTTGT+TGG + chr8.4:41605415-41605434 MS.gene63218:intron 35.0%
TGTAGAATTGGGACAATCAT+CGG + chr8.4:41605925-41605944 MS.gene63218:intron 35.0%
TTAGAAAGTTGAGGTGAGTT+GGG - chr8.4:41605484-41605503 None:intergenic 35.0%
TTAGACATTCACTTGCAGAA+TGG + chr8.4:41605716-41605735 MS.gene63218:intron 35.0%
TTTAGAAAGTTGAGGTGAGT+TGG - chr8.4:41605485-41605504 None:intergenic 35.0%
!! ACAACTAGACATTGGTAATG+TGG + chr8.4:41605976-41605995 MS.gene63218:CDS 35.0%
!! AGCTTCAAAGTTTCTAAGAC+TGG - chr8.4:41606037-41606056 None:intergenic 35.0%
!! CGAAGTTATCAAGTTGAGAT+TGG - chr8.4:41605055-41605074 None:intergenic 35.0%
!! TTTAACTTGAGCTTTGGTGT+GGG + chr8.4:41606640-41606659 MS.gene63218:intron 35.0%
!!! GACTTTGCTATTTTCGTCAA+CGG + chr8.4:41604975-41604994 MS.gene63218:CDS 35.0%
!!! GCGTTTTTCTGGTTTTTTGT+TGG + chr8.4:41606877-41606896 MS.gene63218:CDS 35.0%
AACAGATTCACTTCCAGAAG+AGG - chr8.4:41606266-41606285 None:intergenic 40.0%
AAGTGGGATGCAAGACAATT+AGG - chr8.4:41606764-41606783 None:intergenic 40.0%
AGCCTCACTGATATCACTAT+TGG + chr8.4:41606181-41606200 MS.gene63218:CDS 40.0%
CGGTGAGATCTTTGTAGAAT+TGG + chr8.4:41605913-41605932 MS.gene63218:intron 40.0%
CTCATAGGTATCTTCATCTC+TGG + chr8.4:41604880-41604899 MS.gene63218:CDS 40.0%
GAAGATACCTATGAGCAACA+AGG - chr8.4:41604875-41604894 None:intergenic 40.0%
GATACAGCACCACATCAATT+TGG + chr8.4:41606713-41606732 MS.gene63218:CDS 40.0%
GGCAGAGAGAAGGATATTAA+TGG - chr8.4:41606011-41606030 None:intergenic 40.0%
GGCCAAAGATTCAGTTGAAA+AGG - chr8.4:41606062-41606081 None:intergenic 40.0%
GGTACGTACAAAACAACAAG+TGG - chr8.4:41606436-41606455 None:intergenic 40.0%
GTCCTAAGACCAAATTGATG+TGG - chr8.4:41606725-41606744 None:intergenic 40.0%
TAGTGATATCAGTGAGGCTA+AGG - chr8.4:41606180-41606199 None:intergenic 40.0%
TCAACTCCCATTATTGATGC+AGG + chr8.4:41605377-41605396 MS.gene63218:intron 40.0%
TGAATCTTTGGCCAAGCTTA+GGG + chr8.4:41606069-41606088 MS.gene63218:CDS 40.0%
TGTAATGCACACAGTCTCTA+CGG + chr8.4:41605893-41605912 MS.gene63218:intron 40.0%
TTCTACAATGTGTACTCTCC+CGG + chr8.4:41604924-41604943 MS.gene63218:CDS 40.0%
! CACCACATCAATTTGGTCTT+AGG + chr8.4:41606720-41606739 MS.gene63218:CDS 40.0%
!! CAAAGTTTCTAAGACTGGAC+AGG - chr8.4:41606032-41606051 None:intergenic 40.0%
!! GTTTAACTTGAGCTTTGGTG+TGG + chr8.4:41606639-41606658 MS.gene63218:intron 40.0%
!! TACAGAAAAGCGTCGAAAGT+AGG - chr8.4:41606551-41606570 None:intergenic 40.0%
ACAATGAAAGTTGACCGCGT+TGG + chr8.4:41605324-41605343 MS.gene63218:intron 45.0%
AGAGGAGGTTAGTGCATGAT+AGG - chr8.4:41605190-41605209 None:intergenic 45.0%
AGATGAAGATCCTCAAGGTG+GGG - chr8.4:41605119-41605138 None:intergenic 45.0%
CGAGGAAAACTAGGATCTGA+TGG + chr8.4:41605542-41605561 MS.gene63218:intron 45.0%
CGCCAATAGTGATATCAGTG+AGG - chr8.4:41606186-41606205 None:intergenic 45.0%
CTGAATCTTTGGCCAAGCTT+AGG + chr8.4:41606068-41606087 MS.gene63218:CDS 45.0%
CTTGATGTAGTCTCCTCTTC+TGG + chr8.4:41606250-41606269 MS.gene63218:CDS 45.0%
CTTGGAGGAGAGTGAGTAAA+GGG - chr8.4:41606291-41606310 None:intergenic 45.0%
GAGATGAAGATCCTCAAGGT+GGG - chr8.4:41605120-41605139 None:intergenic 45.0%
GAGGTTAGTGCATGATAGGA+TGG - chr8.4:41605186-41605205 None:intergenic 45.0%
GTAGAGTTTACTCACCAGCA+AGG + chr8.4:41605346-41605365 MS.gene63218:intron 45.0%
GTGAGTAAACTCTACCAACG+CGG - chr8.4:41605341-41605360 None:intergenic 45.0%
TACCTATTTCAGCGAAGGAG+AGG - chr8.4:41605208-41605227 None:intergenic 45.0%
TGTCTTGAGATAGACACACC+TGG + chr8.4:41606145-41606164 MS.gene63218:CDS 45.0%
! AGTTGTACCTTGTTTCCTGC+AGG + chr8.4:41606691-41606710 MS.gene63218:intron 45.0%
!! ATTTAACTCCAGAGAGCGCT+TGG - chr8.4:41606309-41606328 None:intergenic 45.0%
!! TAGAGGATGTTGGCTTGAGA+TGG + chr8.4:41606808-41606827 MS.gene63218:CDS 45.0%
AGTTGATCTCACCACCTTGC+TGG - chr8.4:41605363-41605382 None:intergenic 50.0%
CGAGATGAAGATCCTCAAGG+TGG - chr8.4:41605121-41605140 None:intergenic 50.0%
CTATTTCAGCGAAGGAGAGG+AGG - chr8.4:41605205-41605224 None:intergenic 50.0%
CTCCTCTCCTTCGCTGAAAT+AGG + chr8.4:41605203-41605222 MS.gene63218:CDS 50.0%
CTCTTCTCGTCTGAGGATCA+AGG + chr8.4:41605143-41605162 MS.gene63218:CDS 50.0%
GAGCGAGATGAAGATCCTCA+AGG - chr8.4:41605124-41605143 None:intergenic 50.0%
GAGTTTACTCACCAGCAAGG+TGG + chr8.4:41605349-41605368 MS.gene63218:intron 50.0%
GCTGTATCCTGCAGGAAACA+AGG - chr8.4:41606701-41606720 None:intergenic 50.0%
GCTTGGAGGAGAGTGAGTAA+AGG - chr8.4:41606292-41606311 None:intergenic 50.0%
GGAAGATGGAACCCTAAGCT+TGG - chr8.4:41606083-41606102 None:intergenic 50.0%
GTGCAAGTTCCCAACAGAAG+TGG - chr8.4:41606212-41606231 None:intergenic 50.0%
TACGACATACCCGCGACATT+CGG + chr8.4:41605009-41605028 MS.gene63218:CDS 50.0%
TGCATGATAGGATGGCGAGA+GGG - chr8.4:41605178-41605197 None:intergenic 50.0%
TGTTGGGAACTTGCACAACG+TGG + chr8.4:41606216-41606235 MS.gene63218:CDS 50.0%
! AGGCTAAGGTACTTGAGACC+AGG - chr8.4:41606166-41606185 None:intergenic 50.0%
! ATTGGCGATGCCACTTCTGT+TGG + chr8.4:41606199-41606218 MS.gene63218:CDS 50.0%
! TTGGCGATGCCACTTCTGTT+GGG + chr8.4:41606200-41606219 MS.gene63218:CDS 50.0%
ACGCATCTTCCGAATGTCGC+GGG - chr8.4:41605021-41605040 None:intergenic 55.0%
AGACTGGACAGGCAGAGAGA+AGG - chr8.4:41606021-41606040 None:intergenic 55.0%
CGGAAGATGCGTCTCTCTTG+CGG + chr8.4:41605029-41605048 MS.gene63218:CDS 55.0%
CTGAACGCTCTGTTGACACG+TGG + chr8.4:41605075-41605094 MS.gene63218:CDS 55.0%
GACGCATCTTCCGAATGTCG+CGG - chr8.4:41605022-41605041 None:intergenic 55.0%
GTGCATGATAGGATGGCGAG+AGG - chr8.4:41605179-41605198 None:intergenic 55.0%
TCTCGCTCTCTTCTCGTCTG+AGG + chr8.4:41605136-41605155 MS.gene63218:CDS 55.0%
TGGGAACTTGCACAACGTGG+AGG + chr8.4:41606219-41606238 MS.gene63218:CDS 55.0%
!! GATGTGGTGCTGTATCCTGC+AGG - chr8.4:41606709-41606728 None:intergenic 55.0%
!! TAACTCCAGAGAGCGCTTGG+AGG - chr8.4:41606306-41606325 None:intergenic 55.0%
ACTCTCCTCCAAGCGCTCTC+TGG + chr8.4:41606298-41606317 MS.gene63218:CDS 60.0%
AGAGACCTGCCTTGTCGCTC+GGG + chr8.4:41605447-41605466 MS.gene63218:intron 60.0%
ATCCTCAAGGTGGGGCCCAA+CGG - chr8.4:41605111-41605130 None:intergenic 60.0%
CTAGAAGCTCCCGAGCGACA+AGG - chr8.4:41605459-41605478 None:intergenic 60.0%
GAGAGACCTGCCTTGTCGCT+CGG + chr8.4:41605446-41605465 MS.gene63218:intron 60.0%
AAGCTCCCGAGCGACAAGGC+AGG - chr8.4:41605455-41605474 None:intergenic 65.0%
ACGTGGATCCGCACTGCCGT+TGG + chr8.4:41605092-41605111 MS.gene63218:CDS 65.0%
CGGCAGACAGTAGATCCCCG+TGG - chr8.4:41606956-41606975 None:intergenic 65.0%
CGTGGATCCGCACTGCCGTT+GGG + chr8.4:41605093-41605112 MS.gene63218:CDS 65.0%
! GTGTACTCTCCCGGCGTCGA+CGG + chr8.4:41604933-41604952 MS.gene63218:CDS 65.0%
TGCCGTTGGGCCCCACCTTG+AGG + chr8.4:41605106-41605125 MS.gene63218:CDS 70.0%
GGTGGGGCCCAACGGCAGTG+CGG - chr8.4:41605103-41605122 None:intergenic 75.0%
TTCGGCCTCGCCGTCGACGC+CGG - chr8.4:41604946-41604965 None:intergenic 75.0%
! TCGGCCTCGCCGTCGACGCC+GGG - chr8.4:41604945-41604964 None:intergenic 80.0%
!!! CTCTCCCGGCGTCGACGGCG+AGG + chr8.4:41604938-41604957 MS.gene63218:CDS 80.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 41604867 41606991 41604867 ID=MS.gene63218
chr8.4 mRNA 41604867 41606991 41604867 ID=MS.gene63218.t1;Parent=MS.gene63218
chr8.4 exon 41604867 41605224 41604867 ID=MS.gene63218.t1.exon1;Parent=MS.gene63218.t1
chr8.4 CDS 41604867 41605224 41604867 ID=cds.MS.gene63218.t1;Parent=MS.gene63218.t1
chr8.4 exon 41605930 41606357 41605930 ID=MS.gene63218.t1.exon2;Parent=MS.gene63218.t1
chr8.4 CDS 41605930 41606357 41605930 ID=cds.MS.gene63218.t1;Parent=MS.gene63218.t1
chr8.4 exon 41606461 41606625 41606461 ID=MS.gene63218.t1.exon3;Parent=MS.gene63218.t1
chr8.4 CDS 41606461 41606625 41606461 ID=cds.MS.gene63218.t1;Parent=MS.gene63218.t1
chr8.4 exon 41606713 41606991 41606713 ID=MS.gene63218.t1.exon4;Parent=MS.gene63218.t1
chr8.4 CDS 41606713 41606991 41606713 ID=cds.MS.gene63218.t1;Parent=MS.gene63218.t1
Gene Sequence

>MS.gene63218

ATGAGCCTTGTTGCTCATAGGTATCTTCATCTCTGGAAATATCTCCAAGCTTTTCATTTCTACAATGTGTACTCTCCCGGCGTCGACGGCGAGGCCGAACATTTCAAAGACTTTGCTATTTTCGTCAACGGCTTTCTCTCTTTACGACATACCCGCGACATTCGGAAGATGCGTCTCTCTTGCGGCCAATCTCAACTTGATAACTTCGCTGAACGCTCTGTTGACACGTGGATCCGCACTGCCGTTGGGCCCCACCTTGAGGATCTTCATCTCGCTCTCTTCTCGTCTGAGGATCAAGGCTTCAAACTCCCTCTCGCCATCCTATCATGCACTAACCTCCTCTCCTTCGCTGAAATAGGTATAATGGGATAACAAATTATTAAAATTATTTTATGAAAAAGTAACAAGATTTATTGATTTTAACAATGAAAGTTGACTCCTTTCAAAAAAAAAAAAAACAATGAAAGTTGACCGCGTTGGTAGAGTTTACTCACCAGCAAGGTGGTGAGATCAACTCCCATTATTGATGCAGGAATCTCTTTGGTAGTTGATCTGTAAGTACGTTTGTTGGGTCTCACTGAGAGACCTGCCTTGTCGCTCGGGAGCTTCTAGAGCCCAACTCACCTCAACTTTCTAAAAAAACATTTATTGATTGTTTGTTATATGAAAATAACTCGAGGAAAACTAGGATCTGATGGAGTAATAATTGGTGATTTTATGATTATTATGCTAATTAAGCAAAGACATTTTCAATTAACAAACCAATTAATAAAAGATATGACAAAACTTGCTAAGGAAAAGACACTATGTCATGTTTTGTAGCATTGTTACATACATTCAACATTATTCTTAGACATTCACTTGCAGAATGGCTTTTTATTCTACTATTGTACTGGTATTTATTTACAAAAATTACAGTCTTTTTTAGAGCTTCAAAGATATTTCTTTCAGTCATTTCTTTATTTATTATTTGCACACTTGTTCTTGTTAAATGTGACATGATAAATGATGATAACATAAGATTTGTGTAATGCACACAGTCTCTACGGTGAGATCTTTGTAGAATTGGGACAATCATCGGCTGTTTGTTTACCATCATTGAAGAGATTACAACTAGACATTGGTAATGTGGATGTGAATGCCATTAATATCCTTCTCTCTGCCTGTCCAGTCTTAGAAACTTTGAAGCTTTCCTTTTCAACTGAATCTTTGGCCAAGCTTAGGGTTCCATCTTCCTTGAAGAGTTTTGAATTTACTGTAGAGAATGAAATTGAAGTTTGTCTTGAGATAGACACACCTGGTCTCAAGTACCTTAGCCTCACTGATATCACTATTGGCGATGCCACTTCTGTTGGGAACTTGCACAACGTGGAGGAAGCATATCTTGATGTAGTCTCCTCTTCTGGAAGTGAATCTGTTGACCCTTTACTCACTCTCCTCCAAGCGCTCTCTGGAGTTAAATGTCTAGTGTTTAGTTGTAATACAACAATGGTGAGTGCAATTTTCGTTATCCATGATTATATATTCCTTTATATATTTAGTAGTCTTCTGTTAATTCTTGTATCTCCACTTGTTGTTTTGTACGTACCAAAAGAGATTATTAGGTAGTCAACGTATTATAGATTTTTCGAAAATGCACTTTTCGGAATTTCGCTGTTTACGACATCTAGAGCTCATTCTTCCTACTTTCGACGCTTTTCTGTACGTCGTGCTTCAGAAATGTCCTATACTTGAAGCTCTTATAATTCAGAATGACATGGTTCGTGAATTGAGTTTAACTTGAGCTTTGGTGTGGGTGTGTGTTGTTTGTTTGATTATTTGGTGAGTTGTACCTTGTTTCCTGCAGGATACAGCACCACATCAATTTGGTCTTAGGACAAAGCCGAAAAGTGTTCCTAATTGTCTTGCATCCCACTTGACCTATATCCACTTTAAAGGATATAGAGGATGTTGGCTTGAGATGGACTTTGCTAGTTATGTTTTGCATTATGGACTTGTTTTGAAGAAAATGCGTTTTTCTGGTTTTTTGTTGGATGAAAGAAAGAAGTCGACAAAGTACCATTTTCTCAAAAAAATTTCGTATATGCCACGGGGATCTACTGTCTGCCGAGTTAAATATGACTAA
Protein sequence

>MS.gene63218.t1

MSLVAHRYLHLWKYLQAFHFYNVYSPGVDGEAEHFKDFAIFVNGFLSLRHTRDIRKMRLSCGQSQLDNFAERSVDTWIRTAVGPHLEDLHLALFSSEDQGFKLPLAILSCTNLLSFAEIELGQSSAVCLPSLKRLQLDIGNVDVNAINILLSACPVLETLKLSFSTESLAKLRVPSSLKSFEFTVENEIEVCLEIDTPGLKYLSLTDITIGDATSVGNLHNVEEAYLDVVSSSGSESVDPLLTLLQALSGVKCLVFSCNTTMRLLGSQRIIDFSKMHFSEFRCLRHLELILPTFDAFLYVVLQKCPILEALIIQNDMDTAPHQFGLRTKPKSVPNCLASHLTYIHFKGYRGCWLEMDFASYVLHYGLVLKKMRFSGFLLDERKKSTKYHFLKKISYMPRGSTVCRVKYD