AlfalfaGEDB | MS.gene25396

M. sativa cultivar XinJiangDaYe / MS.gene25396

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00646.36 PF08387.13

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25396.t1	KEH19827.1	93.4	456	30	0	1	456	1	456	7.40E-249	869.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25396.t1	Q9FM89	27.0	463	274	17	7	451	6	422	3.7e-23	110.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25396.t1	A0A072TR07	93.4	456	30	0	1	456	1	456	5.4e-249	869.4

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene03750	MS.gene25396	PPI
MS.gene03752	MS.gene25396	PPI
MS.gene055880	MS.gene25396	PPI
MS.gene055883	MS.gene25396	PPI
MS.gene065234	MS.gene25396	PPI
MS.gene065516	MS.gene25396	PPI
MS.gene072547	MS.gene25396	PPI
MS.gene072985	MS.gene25396	PPI
MS.gene09360	MS.gene25396	PPI
MS.gene09362	MS.gene25396	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25396.t1	MTR_8g469020	93.421	456	30	0	1	456	1	456	0.0	868
MS.gene25396.t1	MTR_8g058965	66.444	450	148	2	7	455	13	460	0.0	585
MS.gene25396.t1	MTR_4g050250	64.356	404	131	7	5	397	19	420	2.14e-170	485
MS.gene25396.t1	MTR_8g070950	56.103	467	146	10	7	456	15	439	5.20e-161	462
MS.gene25396.t1	MTR_8g062010	53.712	458	171	7	7	455	9	434	1.45e-152	441
MS.gene25396.t1	MTR_8g062080	51.812	469	174	13	2	455	18	449	1.51e-142	416
MS.gene25396.t1	MTR_8g062310	52.643	454	166	11	7	456	25	433	1.64e-140	410
MS.gene25396.t1	MTR_8g062300	47.746	488	184	11	7	455	26	481	4.28e-128	380
MS.gene25396.t1	MTR_2g083840	43.805	452	234	7	6	451	20	457	3.42e-99	305
MS.gene25396.t1	MTR_8g467790	46.269	402	147	13	7	395	148	493	2.64e-98	304

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25396.t1	AT4G10400	29.605	456	260	20	7	450	2	408	7.63e-27	112
MS.gene25396.t1	AT4G10400	29.605	456	260	20	7	450	2	408	7.63e-27	112
MS.gene25396.t1	AT4G10400	29.605	456	260	20	7	450	2	408	7.63e-27	112
MS.gene25396.t1	AT4G26340	27.848	474	257	18	7	451	2	419	5.98e-25	107
MS.gene25396.t1	AT1G16930	27.235	481	272	18	5	451	13	449	1.26e-23	103
MS.gene25396.t1	AT1G16930	27.235	481	272	18	5	451	13	449	1.26e-23	103
MS.gene25396.t1	AT1G16930	27.235	481	272	18	5	451	13	449	1.26e-23	103
MS.gene25396.t1	AT5G53840	28.233	464	275	18	7	452	18	441	3.40e-23	102
MS.gene25396.t1	AT5G56420	27.273	462	274	17	7	451	6	422	3.42e-23	101
MS.gene25396.t1	AT5G56420	27.273	462	274	17	7	451	6	422	3.42e-23	101

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Find 110 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCACATTGGGCATCAT+TGG	0.426834	8.2:+40094655	MS.gene25396:CDS
AAACGAGATGGGTGCAATTA+AGG	0.284744	8.2:-40093473	None:intergenic
AACCATCACCTTTAAGAACA+TGG	0.553947	8.2:+40095165	MS.gene25396:CDS
AACGGCCAAGAATGTTCAGA+AGG	0.599711	8.2:+40093238	MS.gene25396:CDS
AAGATGTTGAAACTCGGCGC+AGG	0.597067	8.2:+40094382	MS.gene25396:CDS
AAGATTAACTGATATACCAA+GGG	0.602286	8.2:+40095379	MS.gene25396:CDS
AATCTAGAGTTTCGAGTACG+GGG	0.656709	8.2:-40094489	None:intergenic
AATGTCGCGGGATCGACGGA+GGG	0.613106	8.2:-40093289	None:intergenic
AATTCTGGATAATTTAGAAC+AGG	0.399159	8.2:-40094916	None:intergenic
AATTTCAACCTCTTGAAGAA+AGG	0.468007	8.2:-40094553	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTTCAACCTCTTGAAGAA+AGG	-	chr8.2:40094556-40094575	None:intergenic	30.0%
AGAGAGAGATATACATACAT+AGG	-	chr8.2:40095089-40095108	None:intergenic	30.0%
AGATTAACTGATATACCAAG+GGG	+	chr8.2:40095380-40095399	MS.gene25396:CDS	30.0%
AGTTTCAACATCTTCAATGA+TGG	-	chr8.2:40094376-40094395	None:intergenic	30.0%
ATCTTGATCACAGTATTCAA+TGG	-	chr8.2:40093427-40093446	None:intergenic	30.0%
ATTCACATAGAAGGATATCA+AGG	+	chr8.2:40095239-40095258	MS.gene25396:CDS	30.0%
CAGCTGAATCAAATTTAAGT+TGG	-	chr8.2:40095418-40095437	None:intergenic	30.0%
GAAGATTAACTGATATACCA+AGG	+	chr8.2:40095378-40095397	MS.gene25396:CDS	30.0%
GATCAGTTTCTAAATCAACA+GGG	+	chr8.2:40094109-40094128	MS.gene25396:intron	30.0%
TAAAATCCTGAGATGCATTT+GGG	+	chr8.2:40093959-40093978	MS.gene25396:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	40093057	40095448	40093057	ID=MS.gene25396
chr8.2	mRNA	40093057	40095448	40093057	ID=MS.gene25396.t1;Parent=MS.gene25396
chr8.2	exon	40093057	40093502	40093057	ID=MS.gene25396.t1.exon1;Parent=MS.gene25396.t1
chr8.2	CDS	40093057	40093502	40093057	ID=cds.MS.gene25396.t1;Parent=MS.gene25396.t1
chr8.2	exon	40094324	40094810	40094324	ID=MS.gene25396.t1.exon2;Parent=MS.gene25396.t1
chr8.2	CDS	40094324	40094810	40094324	ID=cds.MS.gene25396.t1;Parent=MS.gene25396.t1
chr8.2	exon	40094901	40095050	40094901	ID=MS.gene25396.t1.exon3;Parent=MS.gene25396.t1
chr8.2	CDS	40094901	40095050	40094901	ID=cds.MS.gene25396.t1;Parent=MS.gene25396.t1
chr8.2	exon	40095161	40095448	40095161	ID=MS.gene25396.t1.exon4;Parent=MS.gene25396.t1
chr8.2	CDS	40095161	40095448	40095161	ID=cds.MS.gene25396.t1;Parent=MS.gene25396.t1

Gene Sequence

>MS.gene25396

ATGGCGCCGCCGACCACCGACAGATTAAGCACTCTACCGGATCCACTCCTCTTCCATATACTCTCCTTCCTTCCAACCAGAACTTCCGTGGCGACGACGAGTCTCATCTCTCGCAGGTGGCGAAATCTCTGGCAGAATCTACAAGCCTTCGAATTCTCCGACGACTCACACGAAGACGGTAAACGGCCAAGAATGTTCAGAAGGTTTGCATTCTTCGTCAACGCCGTGCTCTCCCTCCGTCGATCCCGCGACATTAAAAAGTTTCGTCTCACCAGCGGCCTGTTGAATGATGACTGGTTTCGTAGCGATTGCTTTGAGGTATGGGTCCGTGCTGCCATTGGGCCTCAACTTGAAGAACTGTTTGTTTCCATTGAATACTGTGATCAAGATTGGCAAGTTCTGGTTCCTTCTTCTCTCCTTAATTGCACCCATCTCGTTTCCCTCAGGTAACCCTACTCGTTGTTGTAGGTTTGAAATAAACTCGGTTAAAAAGTGTTTATGTGGGCGCTTATCGATAAGTTTGTAGGTCTAAATTCACCGCTCTCAATAATAGGGTTGATAGGTGTATCAGTCGGTTTTAGTTTGAAACAAAATTGATTACTTGATTTATAGTTTATAATGTTGTAGGTGAAATATTTATATGCAAAATTAGGATTGAATTGAGAAACAATGAGAAGAAAATGTATGTAAGTAATCAGGTTTGGTGTCATAAACACTTTTTGGACGTTGTAGGTAAGCTTTAAACGGTCATGTAAGCACCCTTTAACAACAAAGTTGGATGAAAAAAAAATGATCAATATGTTACAAAAAAGAAAATTAGAGGTCTAAGTTGTTCAATTTTACTGACAGTAAGGACCAATTTGAAACTAAAAAAACTAAAAGGACTAATCTGGTACAAACGTTAAAATCCTGAGATGCATTTGGGATTTCGCTTATTTTTTATGTTGGCAAATAAAATGGCATTTTGCTATTTTCGAAAATATGTACTCTGGTCAAAATGTTGTCACATGGTTTAGCGAGTGACAAGAATTCCTGTTTTTGTCGAGTCCCATGATCAGTTTCTAAATCAACAGGGTACAAAAAAAGTTGTGCAGTCCAGTTCCCTGTATTTTAGCAAATCAAATGCACCTCATGAATTTGGCAAGATTCTCACGTTTTAACAATATCCAAAATTTGGTTGCTTTCATGATTTCATCTCTTGTTCTCTCTCTGTTTTTAACATGTATATATAGAATGTTATGTAACATGTGTTTTGAAAAAACTGCAGTCTTGTTGGTGCAATTCATATAATGTTTCAACCTTCTTCAATTCATCTTCCATCATTGAAGATGTTGAAACTCGGCGCAGGTGTTGTGGAATTCGACATAGGCAACACTGTGGAATTCGACAACATAGACACTGTGGATTCATTACTTGTCTTTCTCTCTGGCTGCCCCGTACTCGAAACTCTAGATTGTGTCTTTTGTGCTAGTTTCTTGGCCAATGTTCCAGTGTCACCTTTCTTCAAGAGGTTGAAATTAACTGGCGTAAATTTCTCTTGGACTTGTCTTCAAATAGATTCTGACTGGCTCTGTGTAGACGACAGTCGTAGCACGGGCAAAACCACATTGGGCATCATTGGAAACTTGCAGAGTGTGGAGGAAGCATATTTTGATGTCTTTTCCCTCCGTGAAAGTGAATTTGTGGACCCTGTGCTCAGCTACCTCCGAAATAATGGTGATATACATCTACTTATGCGTCATTTCACATCAAAGGTGAAGTTTTATTATTATGGTTTATATGTATTTATATATGTACTTCTGTACTAATGTTGTGCCATCACTTGTGATTATGCGTACAAGCAGCGGCCACTTCGCACCCCTGTTCTAAATTATCCAGAATTTTGCAATTTACATCATCTAAAGTTCATTCTTCCCTGCTTCAACATGAGTCTTCTGCTCAACGTGCTTGAGAAATGTCATGTGCTTCAAGTTCTCTTAATTCAGAGTAGCAAGGTTTGCTTTATTTAAATTAATTTTTGGTCTATCTACCTATGTATGTATATCTCTCTCTCTCTATATACACACAAATATAATTGAGTCATCGTGTCCCTTGTTGCTGCTAGGAGGAACCATCACCTTTAAGAACATGGGAGCCAAAGTCAACAACAGTTCCTAAATGTTTCAAATCCCAACTGACCTTTATTCACATAGAAGGATATCAAGGATTTGAAGATGAGCTGGCATTTGCTGAATATGTTTTGCGGAACGGACTTGTTTTACACACAATGCTCGTTTTTTTCGATACATCAATGGACCTAACTAACAAGAACTGTTCTATCAGAAGATTAACTGATATACCAAGGGGCTCTGTCACATGCCAACTTAAATTTGATTCAGCTGTGTCTCCATAA

Protein sequence

>MS.gene25396.t1

MAPPTTDRLSTLPDPLLFHILSFLPTRTSVATTSLISRRWRNLWQNLQAFEFSDDSHEDGKRPRMFRRFAFFVNAVLSLRRSRDIKKFRLTSGLLNDDWFRSDCFEVWVRAAIGPQLEELFVSIEYCDQDWQVLVPSSLLNCTHLVSLSLVGAIHIMFQPSSIHLPSLKMLKLGAGVVEFDIGNTVEFDNIDTVDSLLVFLSGCPVLETLDCVFCASFLANVPVSPFFKRLKLTGVNFSWTCLQIDSDWLCVDDSRSTGKTTLGIIGNLQSVEEAYFDVFSLRESEFVDPVLSYLRNNGDIHLLMRHFTSKRPLRTPVLNYPEFCNLHHLKFILPCFNMSLLLNVLEKCHVLQVLLIQSSKEEPSPLRTWEPKSTTVPKCFKSQLTFIHIEGYQGFEDELAFAEYVLRNGLVLHTMLVFFDTSMDLTNKNCSIRRLTDIPRGSVTCQLKFDSAVSP