AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63104

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63104.t1 MTR_8g062080 87.032 401 33 5 36 429 62 450 0.0 688
MS.gene63104.t1 MTR_8g070950 55.789 380 138 8 58 428 80 438 2.59e-132 388
MS.gene63104.t1 MTR_8g062010 54.830 383 140 9 57 428 74 434 9.22e-123 363
MS.gene63104.t1 MTR_8g062310 52.022 371 150 8 57 424 83 428 2.19e-113 339
MS.gene63104.t1 MTR_8g062300 48.434 415 152 10 58 429 87 482 9.30e-111 334
MS.gene63104.t1 MTR_8g469020 46.682 422 162 13 49 428 55 455 1.10e-110 333
MS.gene63104.t1 MTR_8g058965 48.936 423 166 13 39 429 57 461 3.76e-109 330
MS.gene63104.t1 MTR_4g050250 46.571 350 133 11 57 370 89 420 9.94e-82 258
MS.gene63104.t1 MTR_2g086020 44.652 374 116 9 58 428 34 319 2.93e-78 245
MS.gene63104.t1 MTR_8g467790 44.753 324 121 6 57 368 216 493 2.03e-74 241
MS.gene63104.t1 MTR_2g083840 39.409 406 199 12 44 424 74 457 3.94e-65 216
MS.gene63104.t1 MTR_8g062270 36.294 394 197 17 58 423 86 453 8.49e-50 175
MS.gene63104.t1 MTR_5g092960 35.623 393 208 13 58 423 87 461 2.17e-49 174
MS.gene63104.t1 MTR_8g468810 35.806 391 209 14 58 424 91 463 2.22e-49 174
MS.gene63104.t1 MTR_8g014260 35.787 394 207 17 57 424 78 451 4.29e-49 173
MS.gene63104.t1 MTR_8g058970 34.550 411 207 15 58 424 85 477 9.63e-45 162
MS.gene63104.t1 MTR_4g032730 34.343 396 217 13 58 423 84 466 1.05e-42 156
MS.gene63104.t1 MTR_8g468840 34.456 386 214 13 58 422 81 448 1.66e-42 155
MS.gene63104.t1 MTR_8g467800 36.598 388 210 14 58 424 81 453 2.02e-42 155
MS.gene63104.t1 MTR_8g468760 34.615 390 181 15 58 423 79 418 1.75e-39 147
MS.gene63104.t1 MTR_8g445780 37.049 305 153 11 93 377 105 390 1.03e-36 139
MS.gene63104.t1 MTR_4g024710 32.686 309 168 13 137 424 58 347 5.95e-32 124
MS.gene63104.t1 MTR_5g092960 35.593 295 153 9 58 325 87 371 9.70e-31 122
MS.gene63104.t1 MTR_8g014160 31.892 370 195 14 58 424 110 425 1.81e-29 119
MS.gene63104.t1 MTR_8g468810 35.274 292 158 12 58 327 91 373 4.57e-29 117
MS.gene63104.t1 MTR_8g014210 33.942 274 128 11 154 424 8 231 2.42e-27 109
MS.gene63104.t1 MTR_8g077460 39.873 158 82 5 268 424 230 375 1.37e-26 110
MS.gene63104.t1 MTR_8g468810 35.681 213 116 8 58 254 91 298 2.65e-19 88.6
MS.gene63104.t1 MTR_8g468810 35.681 213 116 8 58 254 91 298 3.13e-19 88.6
MS.gene63104.t1 MTR_8g468810 35.681 213 116 8 58 254 91 298 5.59e-19 88.2
MS.gene63104.t1 MTR_4g024745 31.104 299 164 15 128 403 1 280 3.37e-18 84.7
MS.gene63104.t1 MTR_3g099800 53.247 77 35 1 346 421 29 105 8.23e-18 79.0
MS.gene63104.t1 MTR_5g084070 25.326 383 237 14 59 421 71 424 1.74e-13 72.4
MS.gene63104.t1 MTR_5g080710 26.702 382 227 17 59 416 94 446 1.90e-12 69.3
MS.gene63104.t1 MTR_1g053025 23.698 384 240 11 44 421 50 386 3.06e-12 68.2
MS.gene63104.t1 MTR_8g014230 48.485 66 33 1 349 414 31 95 3.14e-12 63.2
MS.gene63104.t1 MTR_7g026500 25.600 375 195 18 67 423 86 394 3.61e-12 68.2
MS.gene63104.t1 MTR_7g056287 28.280 343 172 17 88 423 96 371 9.49e-12 66.6
MS.gene63104.t1 MTR_7g056277 27.299 348 169 16 88 423 352 627 1.16e-11 67.0
MS.gene63104.t1 MTR_8g467800 37.156 218 110 10 58 254 81 292 4.98e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 96 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCCAGCTGTATCTTCTTAA+AGG 0.147661 8.1:-43067243 None:intergenic
AAATTCCAAGTTTATCAAAT+TGG 0.186934 8.1:-43068067 MS.gene63104:CDS
TGAACTTTACATGATCTAAT+TGG 0.261575 8.1:+43067748 None:intergenic
TGATCTAATTGGCGAAATTC+TGG 0.273531 8.1:+43067759 None:intergenic
ACTCACCGTTAAGAACATTT+TGG 0.277522 8.1:-43067506 MS.gene63104:CDS
TTTGCGGAATGGACTTGTTT+TGG 0.284975 8.1:-43067377 MS.gene63104:CDS
ATATTCTTCTATCTGAATAT+AGG 0.302737 8.1:+43067438 None:intergenic
GACCCGATTCTTTACCTCTT+TGG 0.312262 8.1:-43067970 MS.gene63104:CDS
CACTTCTGATTGTCAATTCA+TGG 0.317831 8.1:-43068565 MS.gene63104:CDS
AATTCCAAGTTTATCAAATT+GGG 0.342863 8.1:-43068066 MS.gene63104:CDS
ACAGAACAATACTTATTTGT+TGG 0.364080 8.1:+43067319 None:intergenic
AGAGATAGTTCTTGAAGGTT+TGG 0.365038 8.1:+43068492 None:intergenic
ATGTGGATCTGTGCCGCCAC+TGG 0.377713 8.1:-43068516 MS.gene63104:CDS
AGTCTCAGCCTCTTGATTGA+TGG 0.381502 8.1:+43068252 None:intergenic
CAGAACAATACTTATTTGTT+GGG 0.386148 8.1:+43067320 None:intergenic
AATCGCGACGGCAGAAGAAC+AGG 0.396170 8.1:-43068963 MS.gene63104:CDS
TCAGAAGTTTCCTTTCTCTC+TGG 0.403176 8.1:-43068210 MS.gene63104:CDS
CTGGCACTTGGCAGAGCCCC+TGG 0.408718 8.1:+43067276 None:intergenic
CAGCTGGGTCAAATCTAATC+TGG 0.416399 8.1:+43067257 None:intergenic
CTTCTATCTGAATATAGGTT+AGG 0.418785 8.1:+43067443 None:intergenic
GTTGCTGCAGTTCATCAATA+AGG 0.430718 8.1:+43068425 None:intergenic
TATTTATGTCGATGCTTCAA+TGG 0.432122 8.1:-43067344 MS.gene63104:CDS
TCTCTCTGGCTGCCCCATGC+TGG 0.439355 8.1:-43068196 MS.gene63104:CDS
TCTGAATGTCGCGGGATTTG+CGG 0.443962 8.1:+43068604 None:intergenic
AGGTGGGATTTGAGACATCT+AGG 0.445260 8.1:+43067463 None:intergenic
CACCTTTAAGAAGATACAGC+TGG 0.453055 8.1:+43067241 None:intergenic
CGGTGTCCCTTGTTGCTAAT+AGG 0.453093 8.1:-43067535 MS.gene63104:CDS
TGGCTCCAAAATGTTCTTAA+CGG 0.455373 8.1:+43067501 None:intergenic
CATGGGGCAGCCAGAGAGAA+AGG 0.456904 8.1:+43068200 None:intergenic
AAAGATTACTTGAAATTCCC+AGG 0.457373 8.1:-43067294 MS.gene63104:CDS
GTGTAAACAAATAGTCTTGA+CGG 0.457846 8.1:-43068315 MS.gene63104:intron
ATCTCTAGAGTTTCCAGCAT+GGG 0.460856 8.1:+43068183 None:intergenic
ACGATTTGATCAGCATATGA+TGG 0.461191 8.1:+43068462 None:intergenic
GATACCCAATTTGATAAACT+TGG 0.471060 8.1:+43068062 None:intergenic
CCACCAATCACAATTTCACT+TGG 0.480236 8.1:-43068110 MS.gene63104:CDS
TCTCCAAAGAGGTAAAGAAT+CGG 0.481364 8.1:+43067967 None:intergenic
ATGCTGGAAACTCTAGAGAT+TGG 0.482827 8.1:-43068180 MS.gene63104:CDS
CAGCTTTCATAGTATGTTGG+AGG 0.484738 8.1:-43068034 MS.gene63104:CDS
CTCCAAAGAGGTAAAGAATC+GGG 0.488312 8.1:+43067968 None:intergenic
TTTATCAAATTGGGTATCGT+TGG 0.494998 8.1:-43068057 MS.gene63104:CDS
GAAGATTTGAGTTGAAGCAA+GGG 0.499254 8.1:+43067721 None:intergenic
CCAAGTGAAATTGTGATTGG+TGG 0.503838 8.1:+43068110 None:intergenic
TGATGAGAGATAGTTCTTGA+AGG 0.513322 8.1:+43068487 None:intergenic
TGGCAGCTTTCATAGTATGT+TGG 0.513421 8.1:-43068037 MS.gene63104:CDS
AGAAGATTTGAGTTGAAGCA+AGG 0.523049 8.1:+43067720 None:intergenic
GAATGTCGCGGGATTTGCGG+AGG 0.523251 8.1:+43068607 None:intergenic
AATTCTGGATAATCTAGTAC+AGG 0.523274 8.1:+43067774 None:intergenic
GAAATATTCACTTTCATGCA+AGG 0.529118 8.1:+43067993 None:intergenic
ACTTCTGATTGTCAATTCAT+GGG 0.531540 8.1:-43068564 MS.gene63104:CDS
TCAAGGATGTGAAGATGAGT+TGG 0.532835 8.1:-43067416 MS.gene63104:CDS
TTGAGTCTTATGCAGAAGAA+AGG 0.534615 8.1:+43069076 None:intergenic
TGACGGTGCAATCGAGGTGG+AGG 0.543209 8.1:-43068298 MS.gene63104:CDS
TTCTTGAAGGTTTGGCCCAG+TGG 0.543689 8.1:+43068500 None:intergenic
TGGCACTTGGCAGAGCCCCT+GGG 0.544115 8.1:+43067277 None:intergenic
TTGAAGGTTTGGCCCAGTGG+CGG 0.549847 8.1:+43068503 None:intergenic
ACCTTTAAGAAGATACAGCT+GGG 0.557746 8.1:+43067242 None:intergenic
CTATCTGAATATAGGTTAGG+TGG 0.557900 8.1:+43067446 None:intergenic
ACTCCAAGTGAAATTGTGAT+TGG 0.559448 8.1:+43068107 None:intergenic
TCAATCAAGAGGCTGAGACT+TGG 0.560739 8.1:-43068249 MS.gene63104:CDS
AACCTCGTAACTTCAGTCAA+AGG 0.561117 8.1:+43068138 None:intergenic
ATTCAGATAGAAGAATATCA+AGG 0.564280 8.1:-43067433 MS.gene63104:CDS
ATGGAACTTCTGAATGTCGC+GGG 0.570738 8.1:+43068596 None:intergenic
TGTGGATCTGTGCCGCCACT+GGG 0.574990 8.1:-43068515 MS.gene63104:CDS
TTTACGGCACTGACAAAGAG+TGG 0.578886 8.1:+43069023 None:intergenic
CGTCTTTGTAATCTCCAAAG+AGG 0.580155 8.1:+43067956 None:intergenic
GTCAAATCTAATCTGGCACT+TGG 0.581058 8.1:+43067264 None:intergenic
GCAGCAACCTCGTTTCCCTA+AGG 0.587406 8.1:-43068410 MS.gene63104:intron
CAACATCATCAAGAACTTCG+TGG 0.594799 8.1:+43068994 None:intergenic
TATCTGAATATAGGTTAGGT+GGG 0.596842 8.1:+43067447 None:intergenic
CTGCAGTTCATCAATAAGGA+AGG 0.599461 8.1:+43068429 None:intergenic
AAGATTACTTGAAATTCCCA+GGG 0.599672 8.1:-43067293 MS.gene63104:CDS
AATCAGAAGTGATAGTGAGA+TGG 0.604985 8.1:+43068577 None:intergenic
TAGTCTTGACGGTGCAATCG+AGG 0.607637 8.1:-43068304 MS.gene63104:CDS
ATTCTGGATAATCTAGTACA+GGG 0.610554 8.1:+43067775 None:intergenic
AAAACAACTATGTTGAAATG+TGG 0.611073 8.1:-43068533 MS.gene63104:CDS
TGTCCCTTGTTGCTAATAGG+AGG 0.620022 8.1:-43067532 MS.gene63104:CDS
ACGGCAGAAGAACAGGTTCG+TGG 0.620682 8.1:-43068956 MS.gene63104:intron
AATGTCGCGGGATTTGCGGA+GGG 0.643744 8.1:+43068608 None:intergenic
GATGGAACTTCTGAATGTCG+CGG 0.645532 8.1:+43068595 None:intergenic
TTCTGGATAATCTAGTACAG+GGG 0.649920 8.1:+43067776 None:intergenic
TACCTTTGACTGAAGTTACG+AGG 0.650580 8.1:-43068140 MS.gene63104:CDS
TCTCATAATTCAGAGCGACA+AGG 0.650946 8.1:-43067661 MS.gene63104:intron
GGAGATTACAAAGACGATGG+CGG 0.653622 8.1:-43067949 MS.gene63104:CDS
CTAGGAACTGTTATTGACTG+TGG 0.654479 8.1:+43067481 None:intergenic
TGTTCCTCCTATTAGCAACA+AGG 0.657108 8.1:+43067528 None:intergenic
TTTGGAGATTACAAAGACGA+TGG 0.657677 8.1:-43067952 MS.gene63104:CDS
TCTTGACGGTGCAATCGAGG+TGG 0.658898 8.1:-43068301 MS.gene63104:CDS
TCTCTAGAGTTTCCAGCATG+GGG 0.662773 8.1:+43068184 None:intergenic
GTTCCTCCTATTAGCAACAA+GGG 0.664196 8.1:+43067529 None:intergenic
GATGTGTCACTCGACATCAA+AGG 0.666423 8.1:-43067908 MS.gene63104:intron
AATCTCTAGAGTTTCCAGCA+TGG 0.669247 8.1:+43068182 None:intergenic
GAAGTTACCTTAGGGAAACG+AGG 0.680454 8.1:+43068403 None:intergenic
GTTGTGATTGTTAATCGCGA+CGG 0.680756 8.1:-43068975 MS.gene63104:CDS
GCATAAGACTCAACAGACGA+CGG 0.710403 8.1:-43069066 MS.gene63104:CDS
CATCATCAAGAACTTCGTGG+TGG 0.731051 8.1:+43068997 None:intergenic
AGATTACTTGAAATTCCCAG+GGG 0.755397 8.1:-43067292 MS.gene63104:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTTAATTGAATCAAATATT+TGG - chr8.1:43068725-43068744 MS.gene63104:intron 15.0%
!! AAATAGTATATGAAAGGTTT+AGG - chr8.1:43067694-43067713 MS.gene63104:CDS 20.0%
!! AAATTCCAAGTTTATCAAAT+TGG - chr8.1:43068280-43068299 MS.gene63104:CDS 20.0%
!! AATTCCAAGTTTATCAAATT+GGG - chr8.1:43068281-43068300 MS.gene63104:CDS 20.0%
!! ATATTCTTCTATCTGAATAT+AGG + chr8.1:43068912-43068931 None:intergenic 20.0%
!! ATGTTACAAACATATTTACT+TGG - chr8.1:43067990-43068009 MS.gene63104:CDS 20.0%
!! GTTATTGATGTCATATTAAA+GGG - chr8.1:43067490-43067509 MS.gene63104:CDS 20.0%
!! TACATGATAAAAACAAGTAA+TGG + chr8.1:43068531-43068550 None:intergenic 20.0%
!!! CAAATATATTTTTTGAGTCA+CGG - chr8.1:43068792-43068811 MS.gene63104:intron 20.0%
! AAAACAACTATGTTGAAATG+TGG - chr8.1:43067814-43067833 MS.gene63104:intron 25.0%
! ACAGAACAATACTTATTTGT+TGG + chr8.1:43069031-43069050 None:intergenic 25.0%
! ACATGTTACATCAATACATT+GGG + chr8.1:43067552-43067571 None:intergenic 25.0%
! AGACTAATTCCAACATAATT+TGG - chr8.1:43067651-43067670 MS.gene63104:intron 25.0%
! ATTCAGATAGAAGAATATCA+AGG - chr8.1:43068914-43068933 MS.gene63104:intron 25.0%
! CAGAACAATACTTATTTGTT+GGG + chr8.1:43069030-43069049 None:intergenic 25.0%
! CTTACATCAAGTTAACTAAT+AGG + chr8.1:43067605-43067624 None:intergenic 25.0%
! CTTTAAATGAAGTTACCTTA+GGG + chr8.1:43067955-43067974 None:intergenic 25.0%
! GGAGAAAAATAGTATATGAA+AGG - chr8.1:43067688-43067707 MS.gene63104:CDS 25.0%
! GGTTATTGATGTCATATTAA+AGG - chr8.1:43067489-43067508 MS.gene63104:CDS 25.0%
! TAAAGGGAATTGGATAAATT+TGG - chr8.1:43067506-43067525 MS.gene63104:CDS 25.0%
! TGAACTTTACATGATCTAAT+TGG + chr8.1:43068602-43068621 None:intergenic 25.0%
!! AAGAAAGGAACTATGTTTTA+TGG + chr8.1:43067259-43067278 None:intergenic 25.0%
!!! AAGGTGGAGTTTTATTATTA+TGG - chr8.1:43068458-43068477 MS.gene63104:CDS 25.0%
!!! CATTTGCTGAATATATTTTG+CGG - chr8.1:43068954-43068973 MS.gene63104:intron 25.0%
!!! TTTATTTTCCATCAATCAAG+AGG - chr8.1:43068087-43068106 MS.gene63104:CDS 25.0%
AAAGATTACTTGAAATTCCC+AGG - chr8.1:43069053-43069072 MS.gene63104:CDS 30.0%
AAGATTACTTGAAATTCCCA+GGG - chr8.1:43069054-43069073 MS.gene63104:CDS 30.0%
AATTCTGGATAATCTAGTAC+AGG + chr8.1:43068576-43068595 None:intergenic 30.0%
AATTTGGAAGAAGACATGAA+AGG - chr8.1:43067667-43067686 MS.gene63104:CDS 30.0%
ACTTCTGATTGTCAATTCAT+GGG - chr8.1:43067783-43067802 MS.gene63104:CDS 30.0%
ATTCTGGATAATCTAGTACA+GGG + chr8.1:43068575-43068594 None:intergenic 30.0%
CACATGTTACATCAATACAT+TGG + chr8.1:43067553-43067572 None:intergenic 30.0%
CTTCTATCTGAATATAGGTT+AGG + chr8.1:43068907-43068926 None:intergenic 30.0%
GAAATATTCACTTTCATGCA+AGG + chr8.1:43068357-43068376 None:intergenic 30.0%
GATACCCAATTTGATAAACT+TGG + chr8.1:43068288-43068307 None:intergenic 30.0%
GATGTAACATGTGTTTAACA+TGG - chr8.1:43067560-43067579 MS.gene63104:intron 30.0%
GATGTCATATTAAAGGGAAT+TGG - chr8.1:43067496-43067515 MS.gene63104:CDS 30.0%
TATCTGAATATAGGTTAGGT+GGG + chr8.1:43068903-43068922 None:intergenic 30.0%
TATTTATGTCGATGCTTCAA+TGG - chr8.1:43069003-43069022 MS.gene63104:CDS 30.0%
TGTCTTCTTCCAAATTATGT+TGG + chr8.1:43067663-43067682 None:intergenic 30.0%
! GCTTTAAATGAAGTTACCTT+AGG + chr8.1:43067956-43067975 None:intergenic 30.0%
! GTGTAAACAAATAGTCTTGA+CGG - chr8.1:43068032-43068051 MS.gene63104:CDS 30.0%
! TTTATCAAATTGGGTATCGT+TGG - chr8.1:43068290-43068309 MS.gene63104:CDS 30.0%
! TTTTTATCATGTACAAGCAG+TGG - chr8.1:43068538-43068557 MS.gene63104:CDS 30.0%
ACACAGGTAAAATAGAGAGA+GGG + chr8.1:43067468-43067487 None:intergenic 35.0%
ACGATTTGATCAGCATATGA+TGG + chr8.1:43067888-43067907 None:intergenic 35.0%
ACTCCAAGTGAAATTGTGAT+TGG + chr8.1:43068243-43068262 None:intergenic 35.0%
AGAGATAGTTCTTGAAGGTT+TGG + chr8.1:43067858-43067877 None:intergenic 35.0%
AGATTACTTGAAATTCCCAG+GGG - chr8.1:43069055-43069074 MS.gene63104:CDS 35.0%
CACTTCTGATTGTCAATTCA+TGG - chr8.1:43067782-43067801 MS.gene63104:CDS 35.0%
CTATCTGAATATAGGTTAGG+TGG + chr8.1:43068904-43068923 None:intergenic 35.0%
CTTGATGTAAGAAGACTCAA+TGG - chr8.1:43067614-43067633 MS.gene63104:intron 35.0%
TCTCCAAAGAGGTAAAGAAT+CGG + chr8.1:43068383-43068402 None:intergenic 35.0%
TGATCTAATTGGCGAAATTC+TGG + chr8.1:43068591-43068610 None:intergenic 35.0%
TGATGAGAGATAGTTCTTGA+AGG + chr8.1:43067863-43067882 None:intergenic 35.0%
TGGCTCCAAAATGTTCTTAA+CGG + chr8.1:43068849-43068868 None:intergenic 35.0%
TTCTGGATAATCTAGTACAG+GGG + chr8.1:43068574-43068593 None:intergenic 35.0%
TTTGGAGATTACAAAGACGA+TGG - chr8.1:43068395-43068414 MS.gene63104:intron 35.0%
! AATCAGAAGTGATAGTGAGA+TGG + chr8.1:43067773-43067792 None:intergenic 35.0%
! ACTCACCGTTAAGAACATTT+TGG - chr8.1:43068841-43068860 MS.gene63104:intron 35.0%
! GCTGAATATATTTTGCGGAA+TGG - chr8.1:43068959-43068978 MS.gene63104:CDS 35.0%
! TCTCTCTATTTTACCTGTGT+GGG - chr8.1:43067468-43067487 MS.gene63104:CDS 35.0%
! TTGAGTCTTATGCAGAAGAA+AGG + chr8.1:43067274-43067293 None:intergenic 35.0%
!! AGAAGATTTGAGTTGAAGCA+AGG + chr8.1:43068630-43068649 None:intergenic 35.0%
!! GAAGATTTGAGTTGAAGCAA+GGG + chr8.1:43068629-43068648 None:intergenic 35.0%
AACCTCGTAACTTCAGTCAA+AGG + chr8.1:43068212-43068231 None:intergenic 40.0%
ATGACATCAATAACCCACAC+AGG + chr8.1:43067484-43067503 None:intergenic 40.0%
CAACATCATCAAGAACTTCG+TGG + chr8.1:43067356-43067375 None:intergenic 40.0%
CACACAGGTAAAATAGAGAG+AGG + chr8.1:43067469-43067488 None:intergenic 40.0%
CAGCTTTCATAGTATGTTGG+AGG - chr8.1:43068313-43068332 MS.gene63104:intron 40.0%
CCAAGTGAAATTGTGATTGG+TGG + chr8.1:43068240-43068259 None:intergenic 40.0%
CCACCAATCACAATTTCACT+TGG - chr8.1:43068237-43068256 MS.gene63104:CDS 40.0%
CGTCTTTGTAATCTCCAAAG+AGG + chr8.1:43068394-43068413 None:intergenic 40.0%
CTCCAAAGAGGTAAAGAATC+GGG + chr8.1:43068382-43068401 None:intergenic 40.0%
CTGCAGTTCATCAATAAGGA+AGG + chr8.1:43067921-43067940 None:intergenic 40.0%
GTCAAATCTAATCTGGCACT+TGG + chr8.1:43069086-43069105 None:intergenic 40.0%
GTTCCTCCTATTAGCAACAA+GGG + chr8.1:43068821-43068840 None:intergenic 40.0%
GTTGCTGCAGTTCATCAATA+AGG + chr8.1:43067925-43067944 None:intergenic 40.0%
GTTGTGATTGTTAATCGCGA+CGG - chr8.1:43067372-43067391 MS.gene63104:CDS 40.0%
TACCTTTGACTGAAGTTACG+AGG - chr8.1:43068207-43068226 MS.gene63104:CDS 40.0%
TCAAGGATGTGAAGATGAGT+TGG - chr8.1:43068931-43068950 MS.gene63104:intron 40.0%
TCAGAAGTTTCCTTTCTCTC+TGG - chr8.1:43068137-43068156 MS.gene63104:CDS 40.0%
TCTCATAATTCAGAGCGACA+AGG - chr8.1:43068686-43068705 MS.gene63104:intron 40.0%
TGGCAGCTTTCATAGTATGT+TGG - chr8.1:43068310-43068329 MS.gene63104:intron 40.0%
TGTTCCTCCTATTAGCAACA+AGG + chr8.1:43068822-43068841 None:intergenic 40.0%
! ATGCTGGAAACTCTAGAGAT+TGG - chr8.1:43068167-43068186 MS.gene63104:CDS 40.0%
! CTAGGAACTGTTATTGACTG+TGG + chr8.1:43068869-43068888 None:intergenic 40.0%
! CTCTCTCTATTTTACCTGTG+TGG - chr8.1:43067467-43067486 MS.gene63104:CDS 40.0%
!! AACTTCGTGGTGGAGTTTTA+CGG + chr8.1:43067343-43067362 None:intergenic 40.0%
!! AATCTCTAGAGTTTCCAGCA+TGG + chr8.1:43068168-43068187 None:intergenic 40.0%
!! ATCTCTAGAGTTTCCAGCAT+GGG + chr8.1:43068167-43068186 None:intergenic 40.0%
!! CAACGGTCTCATTTTTCCTT+TGG - chr8.1:43067422-43067441 MS.gene63104:CDS 40.0%
!! TTTGCGGAATGGACTTGTTT+TGG - chr8.1:43068970-43068989 MS.gene63104:CDS 40.0%
AACGAGAGAGGCAATACCAA+AGG + chr8.1:43067441-43067460 None:intergenic 45.0%
AGTCTCAGCCTCTTGATTGA+TGG + chr8.1:43068098-43068117 None:intergenic 45.0%
ATGGAACTTCTGAATGTCGC+GGG + chr8.1:43067754-43067773 None:intergenic 45.0%
CAGCTGGGTCAAATCTAATC+TGG + chr8.1:43069093-43069112 None:intergenic 45.0%
CATCATCAAGAACTTCGTGG+TGG + chr8.1:43067353-43067372 None:intergenic 45.0%
GAAGTTACCTTAGGGAAACG+AGG + chr8.1:43067947-43067966 None:intergenic 45.0%
GACCCGATTCTTTACCTCTT+TGG - chr8.1:43068377-43068396 MS.gene63104:intron 45.0%
GATGGAACTTCTGAATGTCG+CGG + chr8.1:43067755-43067774 None:intergenic 45.0%
GCATAAGACTCAACAGACGA+CGG - chr8.1:43067281-43067300 MS.gene63104:CDS 45.0%
GGAGATTACAAAGACGATGG+CGG - chr8.1:43068398-43068417 MS.gene63104:intron 45.0%
GGTTCGTGGTAAGCTAACAA+CGG - chr8.1:43067405-43067424 MS.gene63104:CDS 45.0%
TCAATCAAGAGGCTGAGACT+TGG - chr8.1:43068098-43068117 MS.gene63104:CDS 45.0%
TGTCCCTTGTTGCTAATAGG+AGG - chr8.1:43068815-43068834 MS.gene63104:intron 45.0%
! AGGTGGGATTTGAGACATCT+AGG + chr8.1:43068887-43068906 None:intergenic 45.0%
! GATGTGTCACTCGACATCAA+AGG - chr8.1:43068439-43068458 MS.gene63104:CDS 45.0%
!! TCTCTAGAGTTTCCAGCATG+GGG + chr8.1:43068166-43068185 None:intergenic 45.0%
!! TTTACGGCACTGACAAAGAG+TGG + chr8.1:43067327-43067346 None:intergenic 45.0%
AGAGAGGGAATGAACGAGAG+AGG + chr8.1:43067453-43067472 None:intergenic 50.0%
CGGTGTCCCTTGTTGCTAAT+AGG - chr8.1:43068812-43068831 MS.gene63104:intron 50.0%
TCTGAATGTCGCGGGATTTG+CGG + chr8.1:43067746-43067765 None:intergenic 50.0%
TTCTTGAAGGTTTGGCCCAG+TGG + chr8.1:43067850-43067869 None:intergenic 50.0%
! GTGTCACTCGACATCAAAGG+TGG - chr8.1:43068442-43068461 MS.gene63104:CDS 50.0%
! TAGTCTTGACGGTGCAATCG+AGG - chr8.1:43068043-43068062 MS.gene63104:CDS 50.0%
!!! GCGGAATGGACTTGTTTTGG+AGG - chr8.1:43068973-43068992 MS.gene63104:CDS 50.0%
AATCGCGACGGCAGAAGAAC+AGG - chr8.1:43067384-43067403 MS.gene63104:CDS 55.0%
AATGTCGCGGGATTTGCGGA+GGG + chr8.1:43067742-43067761 None:intergenic 55.0%
ACGGCAGAAGAACAGGTTCG+TGG - chr8.1:43067391-43067410 MS.gene63104:CDS 55.0%
GCAGCAACCTCGTTTCCCTA+AGG - chr8.1:43067937-43067956 MS.gene63104:CDS 55.0%
TCTTGACGGTGCAATCGAGG+TGG - chr8.1:43068046-43068065 MS.gene63104:CDS 55.0%
TTGAAGGTTTGGCCCAGTGG+CGG + chr8.1:43067847-43067866 None:intergenic 55.0%
ATGTGGATCTGTGCCGCCAC+TGG - chr8.1:43067831-43067850 MS.gene63104:intron 60.0%
CATGGGGCAGCCAGAGAGAA+AGG + chr8.1:43068150-43068169 None:intergenic 60.0%
GAATGTCGCGGGATTTGCGG+AGG + chr8.1:43067743-43067762 None:intergenic 60.0%
TGACGGTGCAATCGAGGTGG+AGG - chr8.1:43068049-43068068 MS.gene63104:CDS 60.0%
TGTGGATCTGTGCCGCCACT+GGG - chr8.1:43067832-43067851 MS.gene63104:intron 60.0%
TCTCTCTGGCTGCCCCATGC+TGG - chr8.1:43068151-43068170 MS.gene63104:CDS 65.0%
TGGCACTTGGCAGAGCCCCT+GGG + chr8.1:43069073-43069092 None:intergenic 65.0%
CTGGCACTTGGCAGAGCCCC+TGG + chr8.1:43069074-43069093 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 43067246 43069123 43067246 ID=MS.gene63104
chr8.1 mRNA 43067246 43069123 43067246 ID=MS.gene63104.t1;Parent=MS.gene63104
chr8.1 exon 43068957 43069123 43068957 ID=MS.gene63104.t1.exon1;Parent=MS.gene63104.t1
chr8.1 CDS 43068957 43069123 43068957 ID=cds.MS.gene63104.t1;Parent=MS.gene63104.t1
chr8.1 exon 43068411 43068653 43068411 ID=MS.gene63104.t1.exon2;Parent=MS.gene63104.t1
chr8.1 CDS 43068411 43068653 43068411 ID=cds.MS.gene63104.t1;Parent=MS.gene63104.t1
chr8.1 exon 43067909 43068323 43067909 ID=MS.gene63104.t1.exon3;Parent=MS.gene63104.t1
chr8.1 CDS 43067909 43068323 43067909 ID=cds.MS.gene63104.t1;Parent=MS.gene63104.t1
chr8.1 exon 43067662 43067811 43067662 ID=MS.gene63104.t1.exon4;Parent=MS.gene63104.t1
chr8.1 CDS 43067662 43067811 43067662 ID=cds.MS.gene63104.t1;Parent=MS.gene63104.t1
chr8.1 exon 43067246 43067560 43067246 ID=MS.gene63104.t1.exon5;Parent=MS.gene63104.t1
chr8.1 CDS 43067246 43067560 43067246 ID=cds.MS.gene63104.t1;Parent=MS.gene63104.t1
Gene Sequence

>MS.gene63104

ATGATATCGACCATAAAACATAGTTCCTTTCTTCTGCATAAGACTCAACAGACGACGGAAGTCGCTCAAGTGTCTTGTCCACTCTTTGTCAGTGCCGTAAAACTCCACCACGAAGTTCTTGATGATGTTGTGATTGTTAATCGCGACGGCAGAAGAACAGGTTCGTGGTAAGCTAACAACGGTCTCATTTTTCCTTTGGTATTGCCTCTCTCGTTCATTCCCTCTCTCTATTTTACCTGTGTGGGTTATTGATGTCATATTAAAGGGAATTGGATAAATTTGGATAAATTTTTCTCTATTTTGCCCAATGTATTGATGTAACATGTGTTTAACATGGTAGCTGATTATGAAGCACACCTATTAGTTAACTTGATGTAAGAAGACTCAATGGATTAAGTGATTAGAAGACTAATTCCAACATAATTTGGAAGAAGACATGAAAGGAGAAAAATAGTATATGAAAGGTTTAGGTTTTTCGTTAACTCTGTGCTTGCCCTCCGCAAATCCCGCGACATTCAGAAGTTCCATCTCACTATCACTTCTGATTGTCAATTCATGGGCAACATTGAAAACAACTATGTTGAAATGTGGATCTGTGCCGCCACTGGGCCAAACCTTCAAGAACTATCTCTCATCATTCCATCATATGCTGATCAAATCGTAAAACTCTCTCCTTCCTTATTGATGAACTGCAGCAACCTCGTTTCCCTAAGGTAACTTCATTTAAAGCTATCAACATACAACATGTTACAAACATATTTACTTGGTATTTATAGAATTTCTAATGTGTAAACAAATAGTCTTGACGGTGCAATCGAGGTGGAGGTTAAACATTCTTCAGTTTATTTTCCATCAATCAAGAGGCTGAGACTTGGCGAACTCATTGTAGATTCAGAAGTTTCCTTTCTCTCTGGCTGCCCCATGCTGGAAACTCTAGAGATTGGCTTTTACTTAAATAACATACCTTTGACTGAAGTTACGAGGTTGAAATCCACCAATCACAATTTCACTTGGAGTTACTTTGAGTTTTATGTAAATTCCAAGTTTATCAAATTGGGTATCGTTGGCAGCTTTCATAGTATGTTGGAGGCATTTCTTTATGTCTTTTCCTTGCATGAAAGTGAATATTTCGACCCGATTCTTTACCTCTTTGGAGATTACAAAGACGATGGCGGCGATTATGTTCATCTAAAGATGTGTCACTCGACATCAAAGGTGGAGTTTTATTATTATGGTTTATATGTATTCAATTTCATATATGCAGTTTTGTACTGATATTGTGCCATTACTTGTTTTTATCATGTACAAGCAGTGGACACTTCATGCCCCTGTACTAGATTATCCAGAATTTCGCCAATTAGATCATGTAAAGTTCATTCTCCCTTGCTTCAACTCAAATCTTCTTGTCACTGTGCTCGATAAATGTCATATGCTTAAATTTCTCATAATTCAGAGCGACAAGGTTTGTTGACTTTTAAACTTTAATTGAATCAAATATTTGGTTTGTCTATGTATGTATGCATGTATATATCTATATATACACAATCAAATATATTTTTTGAGTCACGGTGTCCCTTGTTGCTAATAGGAGGAACACTCACCGTTAAGAACATTTTGGAGCCACAGTCAATAACAGTTCCTAGATGTCTCAAATCCCACCTAACCTATATTCAGATAGAAGAATATCAAGGATGTGAAGATGAGTTGGCATTTGCTGAATATATTTTGCGGAATGGACTTGTTTTGGAGGCGATGCTTATTTATGTCGATGCTTCAATGGACCCAACAAATAAGTATTGTTCTGTCAAAAGATTACTTGAAATTCCCAGGGGCTCTGCCAAGTGCCAGATTAGATTTGACCCAGCTGTATCTTCTTAA
Protein sequence

>MS.gene63104.t1

MISTIKHSSFLLHKTQQTTEVAQVSCPLFVSAVKLHHEVLDDVVIVNRDGRRTGSWFFVNSVLALRKSRDIQKFHLTITSDCQFMGNIENNYVEMWICAATGPNLQELSLIIPSYADQIVKLSPSLLMNCSNLVSLSLDGAIEVEVKHSSVYFPSIKRLRLGELIVDSEVSFLSGCPMLETLEIGFYLNNIPLTEVTRLKSTNHNFTWSYFEFYVNSKFIKLGIVGSFHSMLEAFLYVFSLHESEYFDPILYLFGDYKDDGGDYVHLKMCHSTSKWTLHAPVLDYPEFRQLDHVKFILPCFNSNLLVTVLDKCHMLKFLIIQSDKSRCPLLLIGGTLTVKNILEPQSITVPRCLKSHLTYIQIEEYQGCEDELAFAEYILRNGLVLEAMLIYVDASMDPTNKYCSVKRLLEIPRGSAKCQIRFDPAVSS