AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63217

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63217.t1 MTR_8g468810 80.255 314 58 3 44 356 153 463 1.06e-173 493
MS.gene63217.t1 MTR_8g468810 86.207 58 6 1 1 58 1 56 2.73e-23 101
MS.gene63217.t1 MTR_8g058970 66.032 315 104 2 44 356 164 477 1.40e-128 379
MS.gene63217.t1 MTR_8g468810 78.378 222 44 3 44 264 153 371 5.99e-111 330
MS.gene63217.t1 MTR_8g468810 86.207 58 6 1 1 58 1 56 1.08e-23 101
MS.gene63217.t1 MTR_2g083840 58.125 320 125 5 39 356 145 457 8.22e-108 325
MS.gene63217.t1 MTR_8g062270 50.311 322 135 9 44 355 147 453 1.73e-88 275
MS.gene63217.t1 MTR_8g467800 48.867 309 132 9 62 356 157 453 8.48e-78 248
MS.gene63217.t1 MTR_8g468760 48.137 322 120 6 41 355 137 418 1.14e-75 241
MS.gene63217.t1 MTR_4g032730 46.018 339 139 10 44 355 145 466 4.73e-74 238
MS.gene63217.t1 MTR_8g468810 76.364 165 36 2 44 208 153 314 6.86e-74 233
MS.gene63217.t1 MTR_8g468810 86.207 58 6 1 1 58 1 56 3.90e-24 102
MS.gene63217.t1 MTR_8g468810 76.364 165 36 2 44 208 153 314 1.17e-73 233
MS.gene63217.t1 MTR_8g468810 86.207 58 6 1 1 58 1 56 4.73e-24 102
MS.gene63217.t1 MTR_8g468840 47.649 319 146 10 44 354 143 448 1.24e-73 237
MS.gene63217.t1 MTR_8g468810 76.364 165 36 2 44 208 153 314 1.67e-73 233
MS.gene63217.t1 MTR_8g468810 86.207 58 6 1 1 58 1 56 5.63e-24 102
MS.gene63217.t1 MTR_5g092960 51.104 317 128 9 54 355 157 461 1.85e-72 234
MS.gene63217.t1 MTR_8g014260 44.340 318 160 10 44 356 146 451 4.51e-66 217
MS.gene63217.t1 MTR_4g024710 41.860 301 161 9 59 356 58 347 1.53e-57 192
MS.gene63217.t1 MTR_8g445780 45.802 262 131 5 47 306 137 389 1.12e-55 188
MS.gene63217.t1 MTR_8g058965 38.776 343 173 12 38 359 133 459 7.93e-49 172
MS.gene63217.t1 MTR_4g024745 40.942 276 152 7 59 331 12 279 2.26e-47 163
MS.gene63217.t1 MTR_8g469020 37.462 331 175 11 44 356 135 451 1.03e-45 164
MS.gene63217.t1 MTR_8g070950 36.533 323 177 10 44 359 136 437 2.07e-44 160
MS.gene63217.t1 MTR_8g014210 42.149 242 128 6 116 356 1 231 5.20e-44 153
MS.gene63217.t1 MTR_8g062010 38.436 307 157 13 59 356 147 430 2.92e-39 145
MS.gene63217.t1 MTR_8g062080 35.692 325 178 10 39 356 145 445 3.46e-39 145
MS.gene63217.t1 MTR_5g092960 50.226 221 94 6 54 264 157 371 3.73e-38 142
MS.gene63217.t1 MTR_4g050250 39.405 269 131 8 52 298 160 418 4.78e-38 142
MS.gene63217.t1 MTR_8g014160 34.528 307 134 8 54 356 182 425 3.87e-36 137
MS.gene63217.t1 MTR_8g062300 35.577 312 173 9 59 356 180 477 1.25e-35 137
MS.gene63217.t1 MTR_8g062310 35.032 314 178 9 44 356 140 428 4.11e-35 134
MS.gene63217.t1 MTR_8g077460 31.139 395 193 16 20 356 2 375 7.91e-34 130
MS.gene63217.t1 MTR_8g467800 53.103 145 63 3 62 201 157 301 9.91e-33 126
MS.gene63217.t1 MTR_2g086020 42.038 157 83 3 201 356 166 315 1.14e-22 97.8
MS.gene63217.t1 MTR_8g467790 31.801 261 132 9 44 300 277 495 5.92e-19 89.0
MS.gene63217.t1 MTR_5g080710 31.343 268 166 11 86 348 192 446 1.03e-16 82.0
MS.gene63217.t1 MTR_5g084070 29.434 265 168 10 89 348 169 419 2.86e-16 80.5
MS.gene63217.t1 MTR_5g008950 26.110 383 210 13 29 356 1 365 1.40e-15 78.2
MS.gene63217.t1 MTR_8g014230 52.000 75 32 2 272 346 25 95 1.96e-15 72.0
MS.gene63217.t1 MTR_7g098510 27.621 391 217 16 21 356 12 391 5.99e-15 76.3
MS.gene63217.t1 MTR_3g087260 28.869 336 182 11 66 355 132 456 1.74e-14 75.1
MS.gene63217.t1 MTR_0543s0010 25.195 385 213 13 26 355 85 449 1.05e-13 72.8
MS.gene63217.t1 MTR_7g032380 25.266 376 203 12 28 356 12 356 2.44e-13 71.2
MS.gene63217.t1 MTR_3g109982 28.134 359 202 11 37 349 121 469 4.41e-13 71.2
MS.gene63217.t1 MTR_7g098480 26.494 385 221 13 23 355 14 388 5.53e-12 67.4
MS.gene63217.t1 MTR_7g056020 27.105 380 190 12 29 350 18 368 7.48e-12 67.4
MS.gene63217.t1 MTR_7g033915 25.197 381 210 16 25 355 9 364 9.18e-12 66.2
MS.gene63217.t1 MTR_1g108800 26.075 372 188 17 28 353 9 339 9.79e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63217.t1 AT4G26340 29.003 331 186 13 42 356 122 419 1.13e-19 90.9
MS.gene63217.t1 AT5G38570 29.670 273 163 9 89 355 126 375 8.60e-17 81.6
MS.gene63217.t1 AT5G56370 27.597 308 164 14 74 355 146 420 1.17e-16 81.6
MS.gene63217.t1 AT5G56370 27.597 308 164 14 74 355 146 420 1.17e-16 81.6
MS.gene63217.t1 AT5G56420 27.124 306 183 11 66 356 142 422 3.67e-15 77.0
MS.gene63217.t1 AT5G56420 27.124 306 183 11 66 356 142 422 3.67e-15 77.0
MS.gene63217.t1 AT5G56420 27.124 306 183 11 66 356 142 422 3.67e-15 77.0
MS.gene63217.t1 AT5G56820 29.155 343 177 19 44 360 128 430 1.89e-14 75.1
MS.gene63217.t1 AT5G56810 29.467 319 183 15 54 355 142 435 6.60e-14 73.6
MS.gene63217.t1 AT1G66300 30.030 333 185 18 44 353 146 453 1.53e-13 72.4
MS.gene63217.t1 AT1G32375 27.217 327 198 13 44 356 122 422 1.59e-13 72.0
MS.gene63217.t1 AT1G32375 27.217 327 198 13 44 356 122 422 1.59e-13 72.0
MS.gene63217.t1 AT3G54910 27.726 321 200 13 42 356 79 373 7.81e-13 69.7
MS.gene63217.t1 AT3G54910 27.726 321 200 13 42 356 79 373 7.81e-13 69.7
MS.gene63217.t1 AT3G54910 27.726 321 200 13 42 356 79 373 7.81e-13 69.7
MS.gene63217.t1 AT1G16930 26.765 340 178 15 55 356 143 449 1.20e-12 69.7
MS.gene63217.t1 AT1G16930 26.765 340 178 15 55 356 143 449 1.20e-12 69.7
MS.gene63217.t1 AT1G16930 26.765 340 178 15 55 356 143 449 1.20e-12 69.7
MS.gene63217.t1 AT5G22730 27.221 349 183 19 44 353 147 463 2.95e-12 68.6
MS.gene63217.t1 AT5G22730 27.221 349 183 19 44 353 147 463 2.95e-12 68.6
MS.gene63217.t1 AT5G62970 28.713 303 184 14 42 331 144 427 9.57e-12 66.6
MS.gene63217.t1 AT4G10400 25.077 323 200 13 42 355 119 408 2.19e-11 65.5
MS.gene63217.t1 AT4G10400 25.077 323 200 13 42 355 119 408 2.19e-11 65.5
MS.gene63217.t1 AT4G10400 25.077 323 200 13 42 355 119 408 2.19e-11 65.5
MS.gene63217.t1 AT5G56390 26.667 330 199 15 46 355 118 424 2.82e-11 65.5
MS.gene63217.t1 AT5G60610 26.246 301 174 12 43 322 114 387 2.84e-11 65.1
MS.gene63217.t1 AT5G60610 26.246 301 174 12 43 322 114 387 2.84e-11 65.1
MS.gene63217.t1 AT2G26030 27.035 344 199 18 40 353 117 438 5.33e-11 64.3
MS.gene63217.t1 AT2G26030 27.035 344 199 18 40 353 117 438 5.33e-11 64.3
MS.gene63217.t1 AT2G26030 27.035 344 199 18 40 353 44 365 5.46e-11 64.3
MS.gene63217.t1 AT5G38590 27.863 262 155 10 72 321 144 383 5.89e-11 64.3

Find 77 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTCCAATCTTAGAAATTT+TGG 0.135666 8.4:+41596176 MS.gene63217:CDS
TTGACCTATATTCACTTTAA+AGG 0.147428 8.4:+41596934 MS.gene63217:CDS
TTGAAGACAATGCTTATTTC+TGG 0.178189 8.4:+41597018 MS.gene63217:CDS
GTTAAATTTGACCGAGTTCT+AGG 0.204330 8.4:+41597129 MS.gene63217:CDS
TCGAAAATTGTCTGAGAAAA+TGG 0.210666 8.4:-41597077 None:intergenic
CTTGCAGGAGAGTGGGTAAA+AGG 0.305542 8.4:-41596434 None:intergenic
AGTTCCAAAATTTCTAAGAT+TGG 0.318329 8.4:-41596180 None:intergenic
CGGTGAGATCTTTGTGGAAT+TGG 0.324937 8.4:+41596059 MS.gene63217:CDS
AAGTGGGATACAAGACAATT+AGG 0.370823 8.4:-41596913 None:intergenic
ATTCACTTTAAAGGATATAC+AGG 0.383467 8.4:+41596943 MS.gene63217:CDS
CCGAATCTTTGGCCAAGCTT+AGG 0.388638 8.4:+41596214 MS.gene63217:CDS
GGCAGAGAGAAGGATATTAA+TGG 0.390004 8.4:-41596154 None:intergenic
CGAATCTTTGGCCAAGCTTA+GGG 0.393635 8.4:+41596215 MS.gene63217:CDS
TTAAATCTCTTCAAGGAAGA+TGG 0.395265 8.4:-41596240 None:intergenic
ACGTACCAAAAGAGATTATT+AGG 0.395270 8.4:+41596601 MS.gene63217:intron
GGATGGTGAGGGGCAGAGTA+AGG 0.403837 8.4:-41597673 None:intergenic
AGGACGAGTTTCATCATCTA+TGG 0.404732 8.4:+41597719 MS.gene63217:CDS
ACTCTCCTGCAAGCTCTCTC+TGG 0.415568 8.4:+41596444 MS.gene63217:CDS
TGGGAAGGAAGGAAAGTATA+TGG 0.423492 8.4:-41595117 None:intergenic
CAATGAGCCTTGTTTCTCAT+AGG 0.428492 8.4:+41595158 MS.gene63217:CDS
GGCATCGCCAAGTGTGATAT+CGG 0.438417 8.4:-41596334 None:intergenic
CCTAAGCTTGGCCAAAGATT+CGG 0.439078 8.4:-41596214 None:intergenic
AGTAAATTTAAATCTCTTCA+AGG 0.440949 8.4:-41596247 None:intergenic
GGCCAAAGATTCGGTTGAAA+AGG 0.442677 8.4:-41596205 None:intergenic
GATACATCACCACATCAATA+TGG 0.457893 8.4:+41596865 MS.gene63217:CDS
TAATTCCAGAGAGAGCTTGC+AGG 0.458090 8.4:-41596449 None:intergenic
TGAAGCACGTCGTACAGAAA+AGG 0.458324 8.4:-41596712 None:intergenic
CAAAATTTCTAAGATTGGAC+AGG 0.469145 8.4:-41596175 None:intergenic
CTTGTGTTAGTGATGTTATA+AGG 0.476534 8.4:-41597694 None:intergenic
AGAGATTTAAATTTACTGTA+GGG 0.479221 8.4:+41596253 MS.gene63217:CDS
AAGAGATTTAAATTTACTGT+AGG 0.482663 8.4:+41596252 MS.gene63217:CDS
ACTTAGAAATTGTATCAGTC+TGG 0.491084 8.4:-41597646 None:intergenic
TACAGGATGTTCGCGTGAGA+TGG 0.495639 8.4:+41596960 MS.gene63217:CDS
GTGTGATATCGGCGAGGCTA+AGG 0.496306 8.4:-41596323 None:intergenic
TCTCTACGGTGAGATCTTTG+TGG 0.502442 8.4:+41596053 MS.gene63217:CDS
ATATCCTTTAAAGTGAATAT+AGG 0.507843 8.4:-41596938 None:intergenic
GAAGCACGTCGTACAGAAAA+GGG 0.514480 8.4:-41596711 None:intergenic
CCAGGAGAAGAAGAAAATGA+TGG 0.521742 8.4:+41595031 MS.gene63217:CDS
GAACTCGGTCAAATTTAACT+TGG 0.525587 8.4:-41597125 None:intergenic
AGTTGTAATCTCTTCAATGA+TGG 0.525654 8.4:-41596105 None:intergenic
GTGCAAGTCCCCAACAAAAG+TGG 0.536088 8.4:-41596355 None:intergenic
TAACATCACTAACACAAGTC+AGG 0.539952 8.4:+41597699 MS.gene63217:CDS
AGAGAGAGCTTGCAGGAGAG+TGG 0.545504 8.4:-41596442 None:intergenic
TAAAGTGAATATAGGTCAAG+TGG 0.551062 8.4:-41596930 None:intergenic
ATTATGAGAGCTTGAAGCAT+AGG 0.560373 8.4:-41596742 None:intergenic
TACAGAAAAGGGTCGAAAGT+AGG 0.563444 8.4:-41596700 None:intergenic
GAGAGAGCTTGCAGGAGAGT+GGG 0.567276 8.4:-41596441 None:intergenic
TGTGGAATTGGAACAATCGT+CGG 0.568307 8.4:+41596071 MS.gene63217:CDS
TGATGTTATAAGGATGGTGA+GGG 0.573321 8.4:-41597684 None:intergenic
AGTAACAGTAACAGTAACAA+AGG 0.577733 8.4:+41595061 MS.gene63217:CDS
AGATTGGACAGGCAGAGAGA+AGG 0.579911 8.4:-41596164 None:intergenic
TGTAATGTACACAGTCTCTA+CGG 0.579931 8.4:+41596039 MS.gene63217:intron
GGAAGATGGAACCCTAAGCT+TGG 0.579963 8.4:-41596226 None:intergenic
GTTTAGTTGTAATTCAGCAA+TGG 0.580635 8.4:+41596482 MS.gene63217:CDS
CAGGAGAAGAAGAAAATGAT+GGG 0.586707 8.4:+41595032 MS.gene63217:CDS
TGTTAGTGATGTTATAAGGA+TGG 0.589770 8.4:-41597690 None:intergenic
GTGATGTTATAAGGATGGTG+AGG 0.591669 8.4:-41597685 None:intergenic
AGTTCAAAACGAAGAAAACC+AGG 0.596394 8.4:+41595013 MS.gene63217:CDS
TTGTATCAGTCTGGTGAAGG+AGG 0.603809 8.4:-41597637 None:intergenic
CAAGTTAATTACCTAGAACT+CGG 0.603983 8.4:-41597140 None:intergenic
AAATTGTATCAGTCTGGTGA+AGG 0.607936 8.4:-41597640 None:intergenic
AAGAGATTACAACTAGACAT+TGG 0.609472 8.4:+41596114 MS.gene63217:CDS
TGGCAGACAGTAGATCCCCT+TGG 0.612624 8.4:-41597105 None:intergenic
GAAGAAGAAAATGATGGGCA+AGG 0.612809 8.4:+41595037 MS.gene63217:CDS
ACCACATCAATATGGTCTCA+CGG 0.613811 8.4:+41596873 MS.gene63217:CDS
AGCCTCGCCGATATCACACT+TGG 0.620507 8.4:+41596327 MS.gene63217:CDS
ACAACTAGACATTGGTAATG+TGG 0.620834 8.4:+41596122 MS.gene63217:CDS
AGGCTAAGGTACTTAAGACC+AGG 0.629957 8.4:-41596309 None:intergenic
AAAGTGAATATAGGTCAAGT+GGG 0.636598 8.4:-41596929 None:intergenic
TCTCATAATTCAGAATGCGA+AGG 0.644808 8.4:+41596756 MS.gene63217:CDS
TGTTGGGGACTTGCACAGCG+TGG 0.651349 8.4:+41596362 MS.gene63217:CDS
TGGGGACTTGCACAGCGTGG+AGG 0.667537 8.4:+41596365 MS.gene63217:CDS
TTAGATACCTATGAGAAACA+AGG 0.672096 8.4:-41595165 None:intergenic
GCCGTGAGACCATATTGATG+TGG 0.673861 8.4:-41596874 None:intergenic
TGTCTTGAGATAGACACACC+TGG 0.677521 8.4:+41596291 MS.gene63217:CDS
CGCCAAGTGTGATATCGGCG+AGG 0.680205 8.4:-41596329 None:intergenic
GATGTTATAAGGATGGTGAG+GGG 0.724451 8.4:-41597683 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGAAATGAATGAAAATAT+AGG + chr8.4:41597561-41597580 MS.gene63217:intron 15.0%
!! CAAAACTACTAAATATATAA+TGG - chr8.4:41596545-41596564 None:intergenic 15.0%
!!! AATGTTTTTTTAGAAAGTTT+AGG - chr8.4:41595662-41595681 None:intergenic 15.0%
!!! ATTTTCGAAAAATCTATAAT+AGG - chr8.4:41596634-41596653 None:intergenic 15.0%
!!! TATTGAAAAACTCTAAAAAT+AGG + chr8.4:41597305-41597324 MS.gene63217:intron 15.0%
!!! TTTAACTTAAGTTTTATTGT+GGG + chr8.4:41596792-41596811 MS.gene63217:intron 15.0%
!!! TTTTTATTCTAAAATTGTAC+TGG + chr8.4:41595907-41595926 MS.gene63217:intron 15.0%
!! AAGAGATTTAAATTTACTGT+AGG + chr8.4:41596252-41596271 MS.gene63217:CDS 20.0%
!! AATGGAATATATAATACACA+TGG - chr8.4:41596527-41596546 None:intergenic 20.0%
!! AGAGATTTAAATTTACTGTA+GGG + chr8.4:41596253-41596272 MS.gene63217:CDS 20.0%
!! AGTAAATTTAAATCTCTTCA+AGG - chr8.4:41596250-41596269 None:intergenic 20.0%
!! CTAAGTTAATTCACAATAAA+TGG - chr8.4:41597187-41597206 None:intergenic 20.0%
!! TTAGAAGTTATATTATGATG+TGG + chr8.4:41597499-41597518 MS.gene63217:intron 20.0%
!! TTGTCATATCTTTCATTAAT+TGG - chr8.4:41595801-41595820 None:intergenic 20.0%
!!! ATATCCTTTAAAGTGAATAT+AGG - chr8.4:41596941-41596960 None:intergenic 20.0%
!!! GTTTAACTTAAGTTTTATTG+TGG + chr8.4:41596791-41596810 MS.gene63217:intron 20.0%
!!! TTTTTCGAAAATGTACTTTT+CGG + chr8.4:41596642-41596661 MS.gene63217:CDS 20.0%
! AAATGAATGAAAATATAGGC+TGG + chr8.4:41597565-41597584 MS.gene63217:intron 25.0%
! AATAGTAGTAAATCTGTGTT+TGG + chr8.4:41597245-41597264 MS.gene63217:intron 25.0%
! ATAGTAGTAAATCTGTGTTT+GGG + chr8.4:41597246-41597265 MS.gene63217:intron 25.0%
! CGTTTAACTCAATTTAACTA+TGG + chr8.4:41597357-41597376 MS.gene63217:intron 25.0%
! TATTTCGCTGTAACAAATAA+GGG - chr8.4:41595405-41595424 None:intergenic 25.0%
! TTGACCTATATTCACTTTAA+AGG + chr8.4:41596934-41596953 MS.gene63217:CDS 25.0%
!! AAATGTACTTTTCGGAATTT+CGG + chr8.4:41596650-41596669 MS.gene63217:CDS 25.0%
!! ATTCACTTTAAAGGATATAC+AGG + chr8.4:41596943-41596962 MS.gene63217:CDS 25.0%
!!! AGTTCCAAAATTTCTAAGAT+TGG - chr8.4:41596183-41596202 None:intergenic 25.0%
AAAATAACTCGAGGAAAACT+AGG + chr8.4:41595703-41595722 MS.gene63217:intron 30.0%
AAAGTGAATATAGGTCAAGT+GGG - chr8.4:41596932-41596951 None:intergenic 30.0%
AAGAGATTACAACTAGACAT+TGG + chr8.4:41596114-41596133 MS.gene63217:CDS 30.0%
ACGTACCAAAAGAGATTATT+AGG + chr8.4:41596601-41596620 MS.gene63217:intron 30.0%
ACTAACAAAGTCTACATTCA+GGG + chr8.4:41595302-41595321 MS.gene63217:intron 30.0%
ACTTAGAAATTGTATCAGTC+TGG - chr8.4:41597649-41597668 None:intergenic 30.0%
AGTAACAGTAACAGTAACAA+AGG + chr8.4:41595061-41595080 MS.gene63217:CDS 30.0%
AGTTGTAATCTCTTCAATGA+TGG - chr8.4:41596108-41596127 None:intergenic 30.0%
ATACTAGTTGTTTATCGTAG+AGG + chr8.4:41597613-41597632 MS.gene63217:intron 30.0%
ATGGTAGTATTACCATCTTA+TGG + chr8.4:41597376-41597395 MS.gene63217:intron 30.0%
ATTTCGCTGTAACAAATAAG+GGG - chr8.4:41595404-41595423 None:intergenic 30.0%
CAAGTTAATTACCTAGAACT+CGG - chr8.4:41597143-41597162 None:intergenic 30.0%
CACAATAAATGGCATGAAAA+TGG - chr8.4:41597176-41597195 None:intergenic 30.0%
CTATTTCGCTGTAACAAATA+AGG - chr8.4:41595406-41595425 None:intergenic 30.0%
CTTGTGTTAGTGATGTTATA+AGG - chr8.4:41597697-41597716 None:intergenic 30.0%
GTGGTGTTTGTTTGATTATT+TGG + chr8.4:41596818-41596837 MS.gene63217:intron 30.0%
GTTTAGTTGTAATTCAGCAA+TGG + chr8.4:41596482-41596501 MS.gene63217:CDS 30.0%
TAAAGTGAATATAGGTCAAG+TGG - chr8.4:41596933-41596952 None:intergenic 30.0%
TAGTAGTAAATCTGTGTTTG+GGG + chr8.4:41597247-41597266 MS.gene63217:intron 30.0%
TCGAAAATTGTCTGAGAAAA+TGG - chr8.4:41597080-41597099 None:intergenic 30.0%
TGATCTGTATGTACATTAGT+TGG + chr8.4:41595584-41595603 MS.gene63217:intron 30.0%
TGGTAGTATTACCATCTTAT+GGG + chr8.4:41597377-41597396 MS.gene63217:intron 30.0%
TGTTAGTGATGTTATAAGGA+TGG - chr8.4:41597693-41597712 None:intergenic 30.0%
TGTTATCTGAAAATAACTCG+AGG + chr8.4:41595694-41595713 MS.gene63217:intron 30.0%
TTAAATCTCTTCAAGGAAGA+TGG - chr8.4:41596243-41596262 None:intergenic 30.0%
TTAGAAAGTTTAGGTGAGTT+GGG - chr8.4:41595653-41595672 None:intergenic 30.0%
TTAGACATTCACTTGCAAAA+TGG + chr8.4:41595883-41595902 MS.gene63217:intron 30.0%
TTAGATACCTATGAGAAACA+AGG - chr8.4:41595168-41595187 None:intergenic 30.0%
TTGAAGACAATGCTTATTTC+TGG + chr8.4:41597018-41597037 MS.gene63217:CDS 30.0%
TTGCAATTTATCTGCATTCA+GGG + chr8.4:41595331-41595350 MS.gene63217:intron 30.0%
TTTAGAAAGTTTAGGTGAGT+TGG - chr8.4:41595654-41595673 None:intergenic 30.0%
TTTATTCCTTTCCCATAAGA+TGG - chr8.4:41597391-41597410 None:intergenic 30.0%
TTTGCAATTTATCTGCATTC+AGG + chr8.4:41595330-41595349 MS.gene63217:intron 30.0%
! ACAATTTTCGAATATGCCAA+GGG + chr8.4:41597089-41597108 MS.gene63217:CDS 30.0%
! AGACAATTAGGAACACTTTT+CGG - chr8.4:41596904-41596923 None:intergenic 30.0%
! CTGTCCAATCTTAGAAATTT+TGG + chr8.4:41596176-41596195 MS.gene63217:CDS 30.0%
! CTTATTTGTTACAGCGAAAT+AGG + chr8.4:41595404-41595423 MS.gene63217:intron 30.0%
! GACTACCTAATAATCTCTTT+TGG - chr8.4:41596609-41596628 None:intergenic 30.0%
! GCTAGTTATGTTTTGCATTA+TGG + chr8.4:41596988-41597007 MS.gene63217:CDS 30.0%
! TATCAGTAAGATTTGGTCAA+TGG - chr8.4:41597528-41597547 None:intergenic 30.0%
!! CAAAATTTCTAAGATTGGAC+AGG - chr8.4:41596178-41596197 None:intergenic 30.0%
!!! GCTTATTTCTGGTTTTTTGT+CGG + chr8.4:41597029-41597048 MS.gene63217:CDS 30.0%
AAATTGTATCAGTCTGGTGA+AGG - chr8.4:41597643-41597662 None:intergenic 35.0%
AACCCTCTATCAGTAAGATT+TGG - chr8.4:41597535-41597554 None:intergenic 35.0%
AAGTGGGATACAAGACAATT+AGG - chr8.4:41596916-41596935 None:intergenic 35.0%
ACTAACGAAGTTTGCATTCA+GGG + chr8.4:41595270-41595289 MS.gene63217:intron 35.0%
AGATATGACAAAACTCGCTA+AGG + chr8.4:41595810-41595829 MS.gene63217:intron 35.0%
AGTAGTAAATCTGTGTTTGG+GGG + chr8.4:41597248-41597267 MS.gene63217:intron 35.0%
AGTTCAAAACGAAGAAAACC+AGG + chr8.4:41595013-41595032 MS.gene63217:CDS 35.0%
ATCAACTTCCACTATTGATG+TGG + chr8.4:41595545-41595564 MS.gene63217:intron 35.0%
ATCACCAATTACTACTCCAT+CGG - chr8.4:41595731-41595750 None:intergenic 35.0%
ATTATGAGAGCTTGAAGCAT+AGG - chr8.4:41596745-41596764 None:intergenic 35.0%
CAGGAGAAGAAGAAAATGAT+GGG + chr8.4:41595032-41595051 MS.gene63217:CDS 35.0%
GAACTCGGTCAAATTTAACT+TGG - chr8.4:41597128-41597147 None:intergenic 35.0%
GACCAAATCTTACTGATAGA+GGG + chr8.4:41597530-41597549 MS.gene63217:intron 35.0%
GACTAACAAAGTCTACATTC+AGG + chr8.4:41595301-41595320 MS.gene63217:intron 35.0%
GATACATCACCACATCAATA+TGG + chr8.4:41596865-41596884 MS.gene63217:CDS 35.0%
GTATTACCATCTTATGGGAA+AGG + chr8.4:41597382-41597401 MS.gene63217:intron 35.0%
GTTAAATTTGACCGAGTTCT+AGG + chr8.4:41597129-41597148 MS.gene63217:CDS 35.0%
TAACATCACTAACACAAGTC+AGG + chr8.4:41597699-41597718 MS.gene63217:CDS 35.0%
TACAGCGAAATAGGTACAAT+GGG + chr8.4:41595413-41595432 MS.gene63217:intron 35.0%
TCAACTTCCACTATTGATGT+GGG + chr8.4:41595546-41595565 MS.gene63217:intron 35.0%
TCTCATAATTCAGAATGCGA+AGG + chr8.4:41596756-41596775 MS.gene63217:CDS 35.0%
TGACCAAATCTTACTGATAG+AGG + chr8.4:41597529-41597548 MS.gene63217:intron 35.0%
TGATGTTATAAGGATGGTGA+GGG - chr8.4:41597687-41597706 None:intergenic 35.0%
TGTAATGTACACAGTCTCTA+CGG + chr8.4:41596039-41596058 MS.gene63217:intron 35.0%
TTACAGCGAAATAGGTACAA+TGG + chr8.4:41595412-41595431 MS.gene63217:intron 35.0%
! ACAATGAAAGTTGACTCAGT+TGG + chr8.4:41595491-41595510 MS.gene63217:intron 35.0%
! CAATTTTCGAATATGCCAAG+GGG + chr8.4:41597090-41597109 MS.gene63217:CDS 35.0%
! GACAATTTTCGAATATGCCA+AGG + chr8.4:41597088-41597107 MS.gene63217:CDS 35.0%
! TTCCTTTTCAACCGAATCTT+TGG + chr8.4:41596203-41596222 MS.gene63217:CDS 35.0%
!! ACAACTAGACATTGGTAATG+TGG + chr8.4:41596122-41596141 MS.gene63217:CDS 35.0%
!! CGAGTTCTAGGTAATTAACT+TGG + chr8.4:41597141-41597160 MS.gene63217:intron 35.0%
!!! GTACACACAGTTGTTTTTGT+GGG - chr8.4:41595139-41595158 None:intergenic 35.0%
!!! TAAGTTTTATTGTGGGTGTG+TGG + chr8.4:41596799-41596818 MS.gene63217:intron 35.0%
!!! TGTACACACAGTTGTTTTTG+TGG - chr8.4:41595140-41595159 None:intergenic 35.0%
ACCACATCAATATGGTCTCA+CGG + chr8.4:41596873-41596892 MS.gene63217:CDS 40.0%
ACGTACACAACAACAACAAG+TGG - chr8.4:41596585-41596604 None:intergenic 40.0%
ACTAACGGAGTCTACATTCA+GGG + chr8.4:41595238-41595257 MS.gene63217:intron 40.0%
AGAGATTCCCACATCAATAG+TGG - chr8.4:41595556-41595575 None:intergenic 40.0%
AGGACGAGTTTCATCATCTA+TGG + chr8.4:41597719-41597738 MS.gene63217:CDS 40.0%
ATTGCAAAACAGTCACTGCA+TGG + chr8.4:41595194-41595213 MS.gene63217:intron 40.0%
CAACAACAAGTGGAGATACA+AGG - chr8.4:41596575-41596594 None:intergenic 40.0%
CCAGGAGAAGAAGAAAATGA+TGG + chr8.4:41595031-41595050 MS.gene63217:CDS 40.0%
GACTAACGAAGTTTGCATTC+AGG + chr8.4:41595269-41595288 MS.gene63217:intron 40.0%
GAGCTCACAATGCAAAAAAC+TGG - chr8.4:41597274-41597293 None:intergenic 40.0%
GATGTATCCTGCAGAAAACA+AGG - chr8.4:41596853-41596872 None:intergenic 40.0%
GATGTTATAAGGATGGTGAG+GGG - chr8.4:41597686-41597705 None:intergenic 40.0%
GGCAGAGAGAAGGATATTAA+TGG - chr8.4:41596157-41596176 None:intergenic 40.0%
GTGATGTTATAAGGATGGTG+AGG - chr8.4:41597688-41597707 None:intergenic 40.0%
TACAGAAAAGGGTCGAAAGT+AGG - chr8.4:41596703-41596722 None:intergenic 40.0%
TCACTGCATGGTCAATTTCA+GGG + chr8.4:41595206-41595225 MS.gene63217:intron 40.0%
TCAGGGACTAAAGTGACTAA+CGG + chr8.4:41595223-41595242 MS.gene63217:intron 40.0%
TGATGTGGGAATCTCTTTGT+TGG + chr8.4:41595560-41595579 MS.gene63217:intron 40.0%
TGTGGAATTGGAACAATCGT+CGG + chr8.4:41596071-41596090 MS.gene63217:CDS 40.0%
! AAGTTGATCTCATGACCTTG+CGG - chr8.4:41595533-41595552 None:intergenic 40.0%
! AGTCACTTTAGTCCCTGAAT+CGG - chr8.4:41595377-41595396 None:intergenic 40.0%
! CCATCATTTTCTTCTTCTCC+TGG - chr8.4:41595034-41595053 None:intergenic 40.0%
! TGGGAAGGAAGGAAAGTATA+TGG - chr8.4:41595120-41595139 None:intergenic 40.0%
!! CAATGAGCCTTGTTTCTCAT+AGG + chr8.4:41595158-41595177 MS.gene63217:CDS 40.0%
!! GAAGAAGAAAATGATGGGCA+AGG + chr8.4:41595037-41595056 MS.gene63217:CDS 40.0%
!! GCATTGTGAGCTCACTTTTA+AGG + chr8.4:41597281-41597300 MS.gene63217:intron 40.0%
!!! ACACAGTTGTTTTTGTGGGA+AGG - chr8.4:41595135-41595154 None:intergenic 40.0%
!!! AGTTGTACCTTGTTTTCTGC+AGG + chr8.4:41596843-41596862 MS.gene63217:intron 40.0%
!!! AGTTGTTTTTGTGGGAAGGA+AGG - chr8.4:41595131-41595150 None:intergenic 40.0%
ACATTAGTTGGCACTCACAG+AGG + chr8.4:41595596-41595615 MS.gene63217:intron 45.0%
ACTTTAGTCCCTGAATCGGA+AGG - chr8.4:41595373-41595392 None:intergenic 45.0%
AGTTGATCTCATGACCTTGC+GGG - chr8.4:41595532-41595551 None:intergenic 45.0%
CATTAGTTGGCACTCACAGA+GGG + chr8.4:41595597-41595616 MS.gene63217:intron 45.0%
CCTAAGCTTGGCCAAAGATT+CGG - chr8.4:41596217-41596236 None:intergenic 45.0%
CGAATCTTTGGCCAAGCTTA+GGG + chr8.4:41596215-41596234 MS.gene63217:CDS 45.0%
CGGTGAGATCTTTGTGGAAT+TGG + chr8.4:41596059-41596078 MS.gene63217:CDS 45.0%
GAAGCACGTCGTACAGAAAA+GGG - chr8.4:41596714-41596733 None:intergenic 45.0%
GACGAATTCCTTCCGATTCA+GGG + chr8.4:41595362-41595381 MS.gene63217:intron 45.0%
GACTAACGGAGTCTACATTC+AGG + chr8.4:41595237-41595256 MS.gene63217:intron 45.0%
GGATCCGATGGAGTAGTAAT+TGG + chr8.4:41595724-41595743 MS.gene63217:intron 45.0%
GTCACTGCATGGTCAATTTC+AGG + chr8.4:41595205-41595224 MS.gene63217:intron 45.0%
TAATTCCAGAGAGAGCTTGC+AGG - chr8.4:41596452-41596471 None:intergenic 45.0%
TCTCTACGGTGAGATCTTTG+TGG + chr8.4:41596053-41596072 MS.gene63217:CDS 45.0%
TGAAGCACGTCGTACAGAAA+AGG - chr8.4:41596715-41596734 None:intergenic 45.0%
TGACGAATTCCTTCCGATTC+AGG + chr8.4:41595361-41595380 MS.gene63217:intron 45.0%
TGTCTTGAGATAGACACACC+TGG + chr8.4:41596291-41596310 MS.gene63217:CDS 45.0%
TTGTATCAGTCTGGTGAAGG+AGG - chr8.4:41597640-41597659 None:intergenic 45.0%
! AGGCTAAGGTACTTAAGACC+AGG - chr8.4:41596312-41596331 None:intergenic 45.0%
! GGCCAAAGATTCGGTTGAAA+AGG - chr8.4:41596208-41596227 None:intergenic 45.0%
!!! TTGGCGATGCCACTTTTGTT+GGG + chr8.4:41596346-41596365 MS.gene63217:CDS 45.0%
!! AAAAAAAAAAAAAAAAAAAC+TGG - chr8.4:41597331-41597350 None:intergenic 5.0%
!!! GTTTTTTTTTTTTTTTTTTT+TGG + chr8.4:41597331-41597350 MS.gene63217:intron 5.0%
AGATTGGACAGGCAGAGAGA+AGG - chr8.4:41596167-41596186 None:intergenic 50.0%
CCGAATCTTTGGCCAAGCTT+AGG + chr8.4:41596214-41596233 MS.gene63217:CDS 50.0%
CGAGGAAAACTAGGATCCGA+TGG + chr8.4:41595712-41595731 MS.gene63217:intron 50.0%
CTTGCAGGAGAGTGGGTAAA+AGG - chr8.4:41596437-41596456 None:intergenic 50.0%
GCCGTGAGACCATATTGATG+TGG - chr8.4:41596877-41596896 None:intergenic 50.0%
GGAAGATGGAACCCTAAGCT+TGG - chr8.4:41596229-41596248 None:intergenic 50.0%
GGCATCGCCAAGTGTGATAT+CGG - chr8.4:41596337-41596356 None:intergenic 50.0%
GTGCAAGTCCCCAACAAAAG+TGG - chr8.4:41596358-41596377 None:intergenic 50.0%
TACAGGATGTTCGCGTGAGA+TGG + chr8.4:41596960-41596979 MS.gene63217:CDS 50.0%
! AAGTAGTTGACTCACCCGCA+AGG + chr8.4:41595515-41595534 MS.gene63217:intron 50.0%
!!! CTTGGCGATGCCACTTTTGT+TGG + chr8.4:41596345-41596364 MS.gene63217:CDS 50.0%
!!! TGGCGATGCCACTTTTGTTG+GGG + chr8.4:41596347-41596366 MS.gene63217:CDS 50.0%
ACTCTCCTGCAAGCTCTCTC+TGG + chr8.4:41596444-41596463 MS.gene63217:CDS 55.0%
AGAGAGAGCTTGCAGGAGAG+TGG - chr8.4:41596445-41596464 None:intergenic 55.0%
AGCCTCGCCGATATCACACT+TGG + chr8.4:41596327-41596346 MS.gene63217:CDS 55.0%
GAGAGAGCTTGCAGGAGAGT+GGG - chr8.4:41596444-41596463 None:intergenic 55.0%
GTGTGATATCGGCGAGGCTA+AGG - chr8.4:41596326-41596345 None:intergenic 55.0%
TGGCAGACAGTAGATCCCCT+TGG - chr8.4:41597108-41597127 None:intergenic 55.0%
! GTTTAGGTGAGTTGGGCTCC+AGG - chr8.4:41595646-41595665 None:intergenic 55.0%
CGCCAAGTGTGATATCGGCG+AGG - chr8.4:41596332-41596351 None:intergenic 60.0%
GGATGGTGAGGGGCAGAGTA+AGG - chr8.4:41597676-41597695 None:intergenic 60.0%
TGTTGGGGACTTGCACAGCG+TGG + chr8.4:41596362-41596381 MS.gene63217:CDS 60.0%
GAGGGACATGCCTTGTCGCG+CGG + chr8.4:41595615-41595634 MS.gene63217:intron 65.0%
TGGGGACTTGCACAGCGTGG+AGG + chr8.4:41596365-41596384 MS.gene63217:CDS 65.0%
CCAGGAGCTACCGCGCGACA+AGG - chr8.4:41595628-41595647 None:intergenic 70.0%
CCTTGTCGCGCGGTAGCTCC+TGG + chr8.4:41595625-41595644 MS.gene63217:intron 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 41595004 41597763 41595004 ID=MS.gene63217
chr8.4 mRNA 41595004 41597763 41595004 ID=MS.gene63217.t1;Parent=MS.gene63217
chr8.4 exon 41595004 41595179 41595004 ID=MS.gene63217.t1.exon1;Parent=MS.gene63217.t1
chr8.4 CDS 41595004 41595179 41595004 ID=cds.MS.gene63217.t1;Parent=MS.gene63217.t1
chr8.4 exon 41596053 41596503 41596053 ID=MS.gene63217.t1.exon2;Parent=MS.gene63217.t1
chr8.4 CDS 41596053 41596503 41596053 ID=cds.MS.gene63217.t1;Parent=MS.gene63217.t1
chr8.4 exon 41596613 41596777 41596613 ID=MS.gene63217.t1.exon3;Parent=MS.gene63217.t1
chr8.4 CDS 41596613 41596777 41596613 ID=cds.MS.gene63217.t1;Parent=MS.gene63217.t1
chr8.4 exon 41596865 41597150 41596865 ID=MS.gene63217.t1.exon4;Parent=MS.gene63217.t1
chr8.4 CDS 41596865 41597150 41596865 ID=cds.MS.gene63217.t1;Parent=MS.gene63217.t1
chr8.4 exon 41597633 41597763 41597633 ID=MS.gene63217.t1.exon5;Parent=MS.gene63217.t1
chr8.4 CDS 41597633 41597763 41597633 ID=cds.MS.gene63217.t1;Parent=MS.gene63217.t1
Gene Sequence

>MS.gene63217

ATGGAGGAAAGTTCAAAACGAAGAAAACCAGGAGAAGAAGAAAATGATGGGCAAGGCAGTAACAGTAACAGTAACAAAGGCAGATTAAGCAGTCTGTCAGACTCTCTCTTGTGCCATATACTTTCCTTCCTTCCCACAAAAACAACTGTGTGTACAATGAGCCTTGTTTCTCATAGGTATCTAAAGAGAAATTGCAAAACAGTCACTGCATGGTCAATTTCAGGGACTAAAGTGACTAACGGAGTCTACATTCAGGGACTAAATTGACTAACGAAGTTTGCATTCAGGGACTAAATTGACTAACAAAGTCTACATTCAGGGACTATTTTGCAATTTATCTGCATTCAGGGACTAAAGTGACGAATTCCTTCCGATTCAGGGACTAAAGTGACTAATTCCCCTTATTTGTTACAGCGAAATAGGTACAATGGGATAACAGATGATTAAAATTATTTTATGAAAAAAGTAACAAGATTTATTGATTTTAACAATGAAAGTTGACTCAGTTGGTAAGTAGTTGACTCACCCGCAAGGTCATGAGATCAACTTCCACTATTGATGTGGGAATCTCTTTGTTGGTTGATCTGTATGTACATTAGTTGGCACTCACAGAGGGACATGCCTTGTCGCGCGGTAGCTCCTGGAGCCCAACTCACCTAAACTTTCTAAAAAAACATTTATTGATTGTTTTGTTATCTGAAAATAACTCGAGGAAAACTAGGATCCGATGGAGTAGTAATTGGTGATTTTATGATTATTATGCTAATTAAGCAAAGACATTTTCAATTAACATACCAATTAATGAAAGATATGACAAAACTCGCTAAGGAAAAGACGCTATGTCATGTTTTGTTACATTGTTACATACATTTAACATTATTAGACATTCACTTGCAAAATGGCTTTTTATTCTAAAATTGTACTGGTATTTACAAAAAATTAGTCTTTTTTAGAGCTACAAAGATATTTCAGTCATTTTTTTATTTATTCTTCTTGTTAAATGTGACATGATAAATGATGATAACATAAGATTTGTGTAATGTACACAGTCTCTACGGTGAGATCTTTGTGGAATTGGAACAATCGTCGGCTGTTTGTTTACCATCATTGAAGAGATTACAACTAGACATTGGTAATGTGGATGTGAATGCCATTAATATCCTTCTCTCTGCCTGTCCAATCTTAGAAATTTTGGAACTTTCCTTTTCAACCGAATCTTTGGCCAAGCTTAGGGTTCCATCTTCCTTGAAGAGATTTAAATTTACTGTAGGGAATGACATTGAAGTTTGTCTTGAGATAGACACACCTGGTCTTAAGTACCTTAGCCTCGCCGATATCACACTTGGCGATGCCACTTTTGTTGGGGACTTGCACAGCGTGGAGGAAGCATATCTTGATTTAGAGTGCTCTTCTGAAAGTGATTCTGTTGACCTTTTACCCACTCTCCTGCAAGCTCTCTCTGGAATTAAACGTCTAGTGTTTAGTTGTAATTCAGCAATGGTGAGTGCAATTTTCATTATCCATGTGTATTATATATTCCATTATATATTTAGTAGTTTTGTGCTAATCCTTGTATCTCCACTTGTTGTTGTTGTGTACGTACCAAAAGAGATTATTAGGTAGTCAACCTATTATAGATTTTTCGAAAATGTACTTTTCGGAATTTCGGTGTTTACTACATCTAGAGCTCATTCTTCCTACTTTCGACCCTTTTCTGTACGACGTGCTTCAGAAATGTCCTATGCTTCAAGCTCTCATAATTCAGAATGCGAAGGTTCGTAAATAGAGTTTAACTTAAGTTTTATTGTGGGTGTGTGGTGTTTGTTTGATTATTTGGTGAGTTGTACCTTGTTTTCTGCAGGATACATCACCACATCAATATGGTCTCACGGCAAAGCCGAAAAGTGTTCCTAATTGTCTTGTATCCCACTTGACCTATATTCACTTTAAAGGATATACAGGATGTTCGCGTGAGATGGAATTTGCTAGTTATGTTTTGCATTATGGACTTGTTTTGAAGACAATGCTTATTTCTGGTTTTTTGTCGGATGAAAGCAAGAAGTCGACAAAGTACCATTTTCTCAGACAATTTTCGAATATGCCAAGGGGATCTACTGTCTGCCAAGTTAAATTTGACCGAGTTCTAGGTAATTAACTTGGTACGAAGTACCATTTTCATGCCATTTATTGTGAATTAACTTAGTAATTTTGCATTGTGAGCTTCTTTTTTTAATTAGTAATAATAGTAGTAAATCTGTGTTTGGGGGCCAGTTTTTTGCATTGTGAGCTCACTTTTAAGGTTATTGAAAAACTCTAAAAATAGGCCAGTTTTTTTTTTTTTTTTTTTTGGTTGCGTTTAACTCAATTTAACTATGGTAGTATTACCATCTTATGGGAAAGGAATAAATTTCTTATGTTCATGTATCAGTTAAGAATTTTTTAACTTCTGTTTCAATCATATTTTATATTTTCTACTTTCTACAAATGTATTTTATTTAGAAGTTATATTATGATGTGGTTGCCATTGACCAAATCTTACTGATAGAGGGTTCAAATGATAGAAATGAATGAAAATATAGGCTGGTCTTTACTGATGATCTAAAAGAATTATACTAGTTGTTTATCGTAGAGGACCTCCTTCACCAGACTGATACAATTTCTAAGTTATTCCTTACTCTGCCCCTCACCATCCTTATAACATCACTAACACAAGTCAGGACGAGTTTCATCATCTATGGCTCTCTTATTTCTGCTAACTAA
Protein sequence

>MS.gene63217.t1

MEESSKRRKPGEEENDGQGSNSNSNKGRLSSLSDSLLCHILSFLPTKTTVCTMSLVSHSLYGEIFVELEQSSAVCLPSLKRLQLDIGNVDVNAINILLSACPILEILELSFSTESLAKLRVPSSLKRFKFTVGNDIEVCLEIDTPGLKYLSLADITLGDATFVGDLHSVEEAYLDLECSSESDSVDLLPTLLQALSGIKRLVFSCNSAMRLLGSQPIIDFSKMYFSEFRCLLHLELILPTFDPFLYDVLQKCPMLQALIIQNAKDTSPHQYGLTAKPKSVPNCLVSHLTYIHFKGYTGCSREMEFASYVLHYGLVLKTMLISGFLSDESKKSTKYHFLRQFSNMPRGSTVCQVKFDRVLEDLLHQTDTISKLFLTLPLTILITSLTQVRTSFIIYGSLISAN