AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032296

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032296.t1 MTR_8g058970 94.688 433 21 1 1 431 45 477 0.0 823
MS.gene032296.t1 MTR_8g468810 64.583 432 133 4 1 431 51 463 0.0 539
MS.gene032296.t1 MTR_8g468810 65.882 340 96 5 1 339 51 371 3.66e-148 426
MS.gene032296.t1 MTR_2g083840 54.042 433 171 8 3 431 49 457 9.92e-147 426
MS.gene032296.t1 MTR_4g032730 48.035 458 174 15 3 430 43 466 1.05e-123 367
MS.gene032296.t1 MTR_8g468810 64.085 284 83 3 1 284 51 315 3.24e-119 350
MS.gene032296.t1 MTR_8g468810 64.085 284 83 3 1 284 51 315 3.58e-119 350
MS.gene032296.t1 MTR_8g468810 64.085 284 83 3 1 284 51 315 7.83e-119 350
MS.gene032296.t1 MTR_8g062270 47.640 445 186 12 1 430 41 453 1.41e-115 346
MS.gene032296.t1 MTR_8g467800 49.099 444 176 14 2 431 46 453 1.17e-114 343
MS.gene032296.t1 MTR_5g092960 48.879 446 179 14 3 430 47 461 1.37e-113 341
MS.gene032296.t1 MTR_8g468840 45.558 439 196 12 2 428 40 447 1.06e-106 323
MS.gene032296.t1 MTR_8g468760 44.820 444 172 12 1 430 34 418 7.47e-99 302
MS.gene032296.t1 MTR_8g014260 42.202 436 216 15 4 431 44 451 1.63e-95 294
MS.gene032296.t1 MTR_8g445780 44.909 383 172 12 4 381 41 389 2.53e-84 263
MS.gene032296.t1 MTR_8g469020 39.735 453 215 18 2 431 34 451 1.09e-82 261
MS.gene032296.t1 MTR_5g092960 47.429 350 146 11 3 339 47 371 1.12e-77 247
MS.gene032296.t1 MTR_8g058965 38.170 448 228 14 2 431 40 456 6.98e-75 241
MS.gene032296.t1 MTR_8g070950 35.056 445 224 13 1 431 41 434 3.07e-68 223
MS.gene032296.t1 MTR_4g050250 38.987 395 196 9 1 373 47 418 1.95e-67 221
MS.gene032296.t1 MTR_8g062010 36.136 440 228 15 1 431 35 430 2.15e-65 216
MS.gene032296.t1 MTR_8g467800 50.000 288 115 8 2 284 46 309 3.79e-65 213
MS.gene032296.t1 MTR_4g024710 44.040 302 153 10 134 431 58 347 1.96e-64 211
MS.gene032296.t1 MTR_8g062080 35.281 445 226 15 1 431 49 445 1.34e-63 211
MS.gene032296.t1 MTR_8g014160 34.932 438 198 11 2 431 67 425 4.01e-63 209
MS.gene032296.t1 MTR_8g062300 35.217 460 235 13 1 431 52 477 2.72e-62 209
MS.gene032296.t1 MTR_8g062310 35.731 431 218 14 4 431 54 428 9.26e-62 206
MS.gene032296.t1 MTR_4g024745 42.500 280 150 8 132 408 10 281 9.07e-52 176
MS.gene032296.t1 MTR_8g014210 43.388 242 125 6 191 431 1 231 5.43e-49 167
MS.gene032296.t1 MTR_8g077460 30.822 438 193 19 4 431 38 375 5.65e-43 155
MS.gene032296.t1 MTR_8g467790 31.443 388 189 15 1 376 174 496 3.74e-36 139
MS.gene032296.t1 MTR_5g084070 28.505 428 253 16 2 423 39 419 8.71e-28 114
MS.gene032296.t1 MTR_5g080710 26.667 435 259 15 2 423 59 446 2.63e-23 102
MS.gene032296.t1 MTR_2g086020 44.853 136 70 3 298 431 183 315 3.89e-22 96.7
MS.gene032296.t1 MTR_2g086020 30.667 225 115 7 1 224 1 185 5.41e-16 79.0
MS.gene032296.t1 MTR_4g056040 27.407 405 229 14 41 419 99 464 1.60e-19 91.3
MS.gene032296.t1 MTR_5g021550 37.879 132 64 4 4 133 47 162 6.31e-19 84.0
MS.gene032296.t1 MTR_4g056070 28.177 362 201 14 62 381 92 436 8.98e-19 88.6
MS.gene032296.t1 MTR_3g087260 28.375 363 188 13 120 430 114 456 3.25e-18 87.0
MS.gene032296.t1 MTR_8g014290 36.691 139 64 6 4 134 38 160 7.28e-17 78.2
MS.gene032296.t1 MTR_7g098510 35.461 141 80 4 298 431 255 391 1.82e-16 81.3
MS.gene032296.t1 MTR_3g036250 28.870 239 143 6 203 428 139 363 2.71e-16 80.5
MS.gene032296.t1 MTR_7g056433 35.417 144 77 6 297 431 253 389 7.25e-16 79.3
MS.gene032296.t1 MTR_3g108150 25.000 440 227 18 2 430 39 386 1.05e-15 79.0
MS.gene032296.t1 MTR_1g041245 34.667 150 83 5 293 431 241 386 7.66e-15 76.3
MS.gene032296.t1 MTR_1089s0010 26.908 249 154 8 200 431 142 379 8.68e-15 75.9
MS.gene032296.t1 MTR_6g040270 24.208 442 223 21 3 429 35 379 1.01e-14 75.9
MS.gene032296.t1 MTR_7g098570 29.304 273 150 13 181 427 134 389 6.46e-14 73.9
MS.gene032296.t1 MTR_5g058100 27.941 272 168 9 178 430 121 383 7.11e-14 73.2
MS.gene032296.t1 MTR_7g026500 26.383 235 147 7 215 430 167 394 1.57e-13 72.4
MS.gene032296.t1 MTR_7g098480 24.374 439 228 17 2 430 44 388 1.57e-13 72.8
MS.gene032296.t1 MTR_3g108560 37.226 137 73 5 303 430 256 388 3.22e-13 71.2
MS.gene032296.t1 MTR_7g032380 26.059 307 174 12 149 431 79 356 4.18e-13 70.5
MS.gene032296.t1 MTR_1g108800 28.713 303 176 12 154 429 51 340 4.33e-13 70.5
MS.gene032296.t1 MTR_1g085120 25.661 378 198 16 66 431 165 471 5.03e-13 70.9
MS.gene032296.t1 MTR_1g085120 25.132 378 200 15 66 431 240 546 7.88e-13 70.5
MS.gene032296.t1 MTR_4g056030 25.600 375 228 14 41 396 69 411 8.78e-13 70.1
MS.gene032296.t1 MTR_2g020180 35.211 142 69 5 298 431 89 215 9.19e-13 67.8
MS.gene032296.t1 MTR_0543s0010 34.752 141 71 6 303 430 317 449 1.13e-12 69.7
MS.gene032296.t1 MTR_8g014230 44.928 69 37 1 347 415 25 92 1.45e-12 64.3
MS.gene032296.t1 MTR_7g032370 32.374 139 83 3 301 431 217 352 1.61e-12 68.9
MS.gene032296.t1 MTR_4g113810 32.895 152 96 4 85 232 13 162 1.77e-12 65.9
MS.gene032296.t1 MTR_7g033915 34.091 132 79 3 303 430 237 364 2.38e-12 68.6
MS.gene032296.t1 MTR_5g013500 30.000 140 91 3 297 431 228 365 3.07e-12 68.2
MS.gene032296.t1 MTR_5g008950 25.723 311 190 12 153 431 64 365 4.66e-12 67.8
MS.gene032296.t1 MTR_3g109982 28.440 327 175 14 120 398 129 444 5.85e-12 68.2
MS.gene032296.t1 MTR_4g080773 25.120 418 262 18 2 396 45 434 7.08e-12 67.4
MS.gene032296.t1 MTR_4g080773 25.120 418 262 18 2 396 45 434 7.86e-12 67.4
MS.gene032296.t1 MTR_3g105060 33.588 131 75 3 301 431 255 373 1.03e-11 66.6
MS.gene032296.t1 MTR_7g034120 30.827 133 80 3 303 431 184 308 1.26e-11 65.9
MS.gene032296.t1 MTR_6g022500 25.217 345 187 11 92 431 198 476 2.49e-11 65.9
MS.gene032296.t1 MTR_1g108820 28.777 278 147 13 188 429 122 384 2.53e-11 65.5
MS.gene032296.t1 MTR_7g114710 31.034 145 86 3 296 431 225 364 3.09e-11 65.1
MS.gene032296.t1 MTR_4g055580 25.521 384 214 17 42 396 198 538 4.43e-11 65.1
MS.gene032296.t1 MTR_5g012840 34.815 135 73 4 304 431 192 318 6.06e-11 63.9
MS.gene032296.t1 MTR_1377s0010 34.314 102 56 3 301 396 31 127 7.83e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032296.t1 AT1G16930 26.226 469 247 22 2 431 41 449 1.76e-25 108
MS.gene032296.t1 AT1G16930 26.226 469 247 22 2 431 41 449 1.76e-25 108
MS.gene032296.t1 AT1G16930 26.226 469 247 22 2 431 41 449 1.76e-25 108
MS.gene032296.t1 AT4G26340 25.508 443 266 17 2 431 28 419 4.91e-24 103
MS.gene032296.t1 AT5G56420 25.170 441 269 16 2 431 32 422 1.45e-22 99.8
MS.gene032296.t1 AT5G56420 25.170 441 269 16 2 431 32 422 1.45e-22 99.8
MS.gene032296.t1 AT5G56420 25.170 441 269 16 2 431 32 422 1.45e-22 99.8
MS.gene032296.t1 AT5G62970 26.961 408 252 13 32 430 78 448 3.46e-22 99.0
MS.gene032296.t1 AT1G78750 25.280 447 279 15 2 428 44 455 7.22e-22 97.8
MS.gene032296.t1 AT1G78750 25.280 447 279 15 2 428 44 455 7.22e-22 97.8
MS.gene032296.t1 AT5G22720 27.004 474 243 24 2 430 49 464 6.27e-21 95.1
MS.gene032296.t1 AT5G22730 28.302 371 210 14 91 429 118 464 1.45e-19 91.3
MS.gene032296.t1 AT5G22730 28.302 371 210 14 91 429 118 464 1.45e-19 91.3
MS.gene032296.t1 AT1G78760 25.499 451 262 24 2 429 42 441 2.34e-19 90.5
MS.gene032296.t1 AT3G54910 25.313 399 230 16 58 431 18 373 3.20e-18 86.3
MS.gene032296.t1 AT3G54910 25.313 399 230 16 58 431 18 373 3.20e-18 86.3
MS.gene032296.t1 AT3G54910 25.313 399 230 16 58 431 18 373 3.20e-18 86.3
MS.gene032296.t1 AT1G32375 26.685 356 222 13 91 431 91 422 3.40e-18 86.7
MS.gene032296.t1 AT1G32375 26.685 356 222 13 91 431 91 422 3.40e-18 86.7
MS.gene032296.t1 AT1G69630 24.454 458 262 20 2 428 44 448 6.11e-18 86.3
MS.gene032296.t1 AT4G13985 24.078 461 269 18 1 428 44 456 6.33e-18 86.3
MS.gene032296.t1 AT1G51370 26.398 447 246 23 4 428 47 432 8.60e-18 85.5
MS.gene032296.t1 AT5G38570 30.545 275 158 10 164 430 126 375 1.03e-17 84.7
MS.gene032296.t1 AT5G56390 25.055 451 259 19 3 430 30 424 2.44e-17 84.0
MS.gene032296.t1 AT1G13780 27.108 332 202 14 120 428 138 452 5.37e-17 83.2
MS.gene032296.t1 AT5G44950 28.904 301 180 13 148 428 149 435 5.58e-17 83.2
MS.gene032296.t1 AT2G04230 25.446 448 276 15 2 431 39 446 7.67e-17 82.8
MS.gene032296.t1 AT5G56370 25.474 369 230 14 80 430 79 420 9.03e-17 82.4
MS.gene032296.t1 AT5G56370 25.474 369 230 14 80 430 79 420 9.03e-17 82.4
MS.gene032296.t1 AT3G50710 28.453 362 212 20 91 431 92 427 1.10e-16 82.0
MS.gene032296.t1 AT1G80470 25.602 332 206 16 114 428 127 434 1.66e-16 81.6
MS.gene032296.t1 AT4G00160 25.828 453 259 19 2 430 42 441 3.29e-16 80.9
MS.gene032296.t1 AT5G27750 28.729 362 195 17 101 431 128 457 4.37e-16 80.5
MS.gene032296.t1 AT5G56700 27.103 321 197 13 91 396 52 350 7.58e-16 79.0
MS.gene032296.t1 AT4G10400 23.409 440 267 18 2 430 28 408 9.25e-16 79.3
MS.gene032296.t1 AT4G10400 23.409 440 267 18 2 430 28 408 9.25e-16 79.3
MS.gene032296.t1 AT4G10400 23.409 440 267 18 2 430 28 408 9.25e-16 79.3
MS.gene032296.t1 AT5G56820 28.286 350 196 19 101 430 111 425 2.74e-15 77.8
MS.gene032296.t1 AT3G26930 27.548 363 207 17 97 431 64 398 2.97e-15 77.8
MS.gene032296.t1 AT3G26930 27.548 363 207 17 97 431 109 443 3.19e-15 77.8
MS.gene032296.t1 AT5G53635 23.146 445 279 16 3 430 27 425 5.19e-15 77.0
MS.gene032296.t1 AT3G52680 26.542 373 215 15 86 430 103 444 1.12e-14 76.3
MS.gene032296.t1 AT3G26920 27.577 359 214 17 89 430 10 339 1.21e-14 75.1
MS.gene032296.t1 AT3G26920 27.577 359 214 17 89 430 10 339 1.21e-14 75.1
MS.gene032296.t1 AT5G44980 28.254 315 179 16 141 430 141 433 2.52e-14 75.1
MS.gene032296.t1 AT5G44980 28.254 315 179 16 141 430 141 433 2.52e-14 75.1
MS.gene032296.t1 AT5G22670 26.593 361 222 16 94 431 102 442 3.03e-14 74.7
MS.gene032296.t1 AT5G50270 24.824 427 250 18 3 408 30 406 3.91e-14 74.3
MS.gene032296.t1 AT4G13965 27.477 444 246 20 2 428 36 420 4.21e-14 74.3
MS.gene032296.t1 AT5G22660 26.667 375 221 18 86 428 91 443 1.41e-13 72.8
MS.gene032296.t1 AT5G56560 23.810 441 269 16 3 431 33 418 3.08e-13 71.6
MS.gene032296.t1 AT4G00315 25.828 453 272 21 2 430 28 440 3.33e-13 71.6
MS.gene032296.t1 AT5G56400 25.495 455 251 20 4 431 62 455 5.64e-13 70.9
MS.gene032296.t1 AT3G49030 24.390 451 262 19 2 430 47 440 1.04e-12 70.1
MS.gene032296.t1 AT5G56800 27.211 294 170 11 153 430 78 343 1.27e-12 69.3
MS.gene032296.t1 AT5G60610 24.332 337 221 12 75 397 71 387 2.13e-12 68.9
MS.gene032296.t1 AT5G60610 24.332 337 221 12 75 397 71 387 2.13e-12 68.9
MS.gene032296.t1 AT5G38590 26.332 319 195 14 91 396 92 383 2.65e-12 68.6
MS.gene032296.t1 AT5G56325 27.018 285 154 13 170 431 11 264 6.05e-12 66.2
MS.gene032296.t1 AT3G49030 23.744 438 255 16 2 430 47 414 6.83e-12 67.4
MS.gene032296.t1 AT5G22610 27.139 339 202 16 120 428 140 463 1.02e-11 67.0
MS.gene032296.t1 AT1G55660 25.762 361 226 15 94 430 149 491 1.59e-11 66.6
MS.gene032296.t1 AT5G22610 26.462 325 192 14 120 428 140 433 1.61e-11 66.2
MS.gene032296.t1 AT3G54160 26.620 432 246 21 24 428 36 423 4.99e-11 64.7
MS.gene032296.t1 AT3G52690 27.852 298 181 12 147 430 20 297 5.45e-11 63.9
MS.gene032296.t1 AT1G55030 29.110 292 173 12 118 397 131 400 9.85e-11 63.9

Find 100 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAAGAAAATGGTTATTTC+TGG 0.183524 8.2:-47725308 MS.gene032296:CDS
TTGTCCAATATGCACATTAA+AGG 0.261211 8.2:-47725392 MS.gene032296:CDS
CGGTGAGTTTCAAACTCTTC+AGG 0.283312 8.2:+47726059 None:intergenic
ACTTCTCCCACTTCTTCTTT+TGG 0.291320 8.2:+47725273 None:intergenic
ATCATTGGCAAATGTAATTC+TGG 0.307551 8.2:+47725973 None:intergenic
TCTAGTATTGCGATGTTATA+CGG 0.309028 8.2:-47725831 MS.gene032296:CDS
ATACCCTTTAATGTGCATAT+TGG 0.316620 8.2:+47725388 None:intergenic
ACGAAACTTGGCCAGAGATA+TGG 0.324996 8.2:+47726093 None:intergenic
AGACAGTTAGGAACAGTATT+AGG 0.329586 8.2:+47725425 None:intergenic
TTCAAACTCTTCAGGGTAGA+TGG 0.342282 8.2:+47726067 None:intergenic
TGAGCTCTAAATGTAGTAAA+TGG 0.344521 8.2:+47725651 None:intergenic
CAAGTAAGCATGTGGGTCTT+CGG 0.352555 8.2:+47727820 None:intergenic
AGTTCCAGGGTTTCTAAGAT+TGG 0.354019 8.2:+47726127 None:intergenic
TGTAGTAAATGGTGAAATTC+CGG 0.355598 8.2:+47725662 None:intergenic
CCGATCCGGAGCCCGGTTTA+CGG 0.376017 8.2:-47727783 MS.gene032296:CDS
TGGGAGAAAAGGCAAGTTCC+AGG 0.381998 8.2:+47726113 None:intergenic
AAAACGATGAAACTAGATAT+TGG 0.387039 8.2:-47726193 MS.gene032296:CDS
ACCGCCAAGCCACTTTCATT+TGG 0.398775 8.2:+47725452 None:intergenic
GTCCCCAACCGCAGTATCAT+TGG 0.423942 8.2:+47725958 None:intergenic
CGTGGGTCCGATCCGGAGCC+CGG 0.426267 8.2:-47727790 MS.gene032296:CDS
CGAAACTTGGCCAGAGATAT+GGG 0.428106 8.2:+47726094 None:intergenic
CGACAGACATTTCCGTAAAC+CGG 0.432497 8.2:+47727771 None:intergenic
AAGTAAGCATGTGGGTCTTC+GGG 0.436541 8.2:+47727821 None:intergenic
GGCCAGAGATATGGGAGAAA+AGG 0.438101 8.2:+47726102 None:intergenic
CGGAAATGTCTGTCGATGCT+TGG 0.439301 8.2:-47727763 MS.gene032296:CDS
GTTTAATTCCAGTGAGAGCT+TGG 0.442769 8.2:+47725855 None:intergenic
GAGGCTAAGGTATTTGAGAT+TGG 0.443266 8.2:+47725997 None:intergenic
TTCGGAGTTGGCGCCGAGGA+TGG 0.443651 8.2:+47727946 None:intergenic
TTGCCAATGATACTGCGGTT+GGG 0.445868 8.2:-47725961 MS.gene032296:CDS
AGGCTAAGGTATTTGAGATT+GGG 0.448137 8.2:+47725998 None:intergenic
TGTCCAATATGCACATTAAA+GGG 0.450045 8.2:-47725391 MS.gene032296:CDS
CACGTAGATATCATCATCTC+TGG 0.451822 8.2:-47728009 MS.gene032296:CDS
TGCCAATGATACTGCGGTTG+GGG 0.453821 8.2:-47725960 MS.gene032296:CDS
AAGTGAGACACAAGACAGTT+AGG 0.455291 8.2:+47725413 None:intergenic
TACCTGATGGAGAGTAGCTT+AGG 0.460330 8.2:+47727641 None:intergenic
GTTCCAGGGTTTCTAAGATT+GGG 0.463116 8.2:+47726128 None:intergenic
TGTGGAATTAGCACAATCTT+CGG 0.469907 8.2:-47726236 MS.gene032296:CDS
CTTGGAGGAGAATGAGTAAA+GGG 0.471592 8.2:+47725873 None:intergenic
TTATGCTCCTGAAAATGAAT+CGG 0.471616 8.2:-47725900 MS.gene032296:CDS
TCGGAGTTGGCGCCGAGGAT+GGG 0.473211 8.2:+47727947 None:intergenic
GGTGAGTTTCAAACTCTTCA+GGG 0.475730 8.2:+47726060 None:intergenic
TCTGTGGATATCCTTCTCTC+AGG 0.483856 8.2:-47726154 MS.gene032296:CDS
ATGTAATTCTGGTGAGGCTA+AGG 0.489727 8.2:+47725984 None:intergenic
ATTCTCCTCCAAGCTCTCAC+TGG 0.490637 8.2:-47725863 MS.gene032296:CDS
GGTAGATGGAACACGAAACT+TGG 0.490742 8.2:+47726081 None:intergenic
GAAGCGGACCAGCGGCAATA+CGG 0.496795 8.2:+47727738 None:intergenic
GCCCTAAGCTACTCTCCATC+AGG 0.499571 8.2:-47727643 MS.gene032296:intron
ATGCACATTAAAGGGTATCA+AGG 0.500704 8.2:-47725383 MS.gene032296:CDS
GCTTGGATCCGTATTGCCGC+TGG 0.505305 8.2:-47727746 MS.gene032296:CDS
TTTGCCAATGATACTGCGGT+TGG 0.505561 8.2:-47725962 MS.gene032296:CDS
CGTAGTAGAAGTTGAATGAC+TGG 0.506942 8.2:+47727978 None:intergenic
TGCCGCTGGTCCGCTTCTTG+AGG 0.510807 8.2:-47727732 MS.gene032296:CDS
TATGACTCGTGGGTCCGATC+CGG 0.514529 8.2:-47727797 MS.gene032296:CDS
ATTATAAGAGCTTGAAGCAT+AGG 0.517782 8.2:+47725581 None:intergenic
ACCAAATGAAAGTGGCTTGG+CGG 0.520565 8.2:-47725453 MS.gene032296:CDS
ATGAGAGAACAGTGAGAGGA+AGG 0.526517 8.2:+47727666 None:intergenic
CTTGGACCAAAAGAAGAAGT+GGG 0.532722 8.2:-47725279 MS.gene032296:CDS
GCTTGGAGGAGAATGAGTAA+AGG 0.533811 8.2:+47725872 None:intergenic
TCTTGGACCAAAAGAAGAAG+TGG 0.534181 8.2:-47725280 MS.gene032296:CDS
GAGCACAGCGTTGACGAAAA+CGG 0.534757 8.2:+47727907 None:intergenic
CATTTCCGTAAACCGGGCTC+CGG 0.535268 8.2:+47727778 None:intergenic
TCAAGGAGATTCTAGTGAGA+TGG 0.541910 8.2:-47725366 MS.gene032296:CDS
ACCTGATGGAGAGTAGCTTA+GGG 0.542326 8.2:+47727642 None:intergenic
TAGCTCAGTCAAATGTAACT+TGG 0.544825 8.2:+47725201 None:intergenic
TGCTTACTTGTATGACTCGT+GGG 0.545376 8.2:-47727807 MS.gene032296:CDS
ATCACCAAATGAAAGTGGCT+TGG 0.546941 8.2:-47725456 MS.gene032296:CDS
ACGCATCTTACGAACGTCGA+TGG 0.547049 8.2:+47727871 None:intergenic
CGTAAGATGCGTCTCTCATG+TGG 0.558006 8.2:-47727860 MS.gene032296:CDS
CCGTAAACCGGGCTCCGGAT+CGG 0.559131 8.2:+47727783 None:intergenic
ATGCTTACTTGTATGACTCG+TGG 0.559289 8.2:-47727808 MS.gene032296:CDS
CTGCCCAATCTTAGAAACCC+TGG 0.570258 8.2:-47726131 MS.gene032296:CDS
TGTAAGAAGAGACTGGTAGA+TGG 0.572092 8.2:-47725719 MS.gene032296:intron
TAATTCCAGTGAGAGCTTGG+AGG 0.575459 8.2:+47725858 None:intergenic
AGATTCATTTCCTCAAGAAG+CGG 0.582908 8.2:+47727722 None:intergenic
TGGCAGACAGCAGAGCCCCT+CGG 0.585541 8.2:+47725221 None:intergenic
GATACATCACCAAATGAAAG+TGG 0.589613 8.2:-47725461 MS.gene032296:CDS
CTCACCGTTGAGAATGACAT+TGG 0.590348 8.2:-47726043 MS.gene032296:CDS
GGGAGAAAAGGCAAGTTCCA+GGG 0.590931 8.2:+47726114 None:intergenic
GACAGACATTTCCGTAAACC+GGG 0.593014 8.2:+47727772 None:intergenic
TACGACTATAATCCCATCCT+CGG 0.598194 8.2:-47727959 MS.gene032296:CDS
AGCTCCAATGTCATTCTCAA+CGG 0.598655 8.2:+47726039 None:intergenic
TTCCTCAAGAAGCGGACCAG+CGG 0.605098 8.2:+47727730 None:intergenic
GTACAGAAAAGTGTCGAAAG+TGG 0.605540 8.2:+47725622 None:intergenic
TACATTTGCCAATGATACTG+CGG 0.609576 8.2:-47725966 MS.gene032296:CDS
GAGTCATACAAGTAAGCATG+TGG 0.610775 8.2:+47727812 None:intergenic
ATTGCGATGTTATACGGCAA+AGG 0.611405 8.2:-47725825 MS.gene032296:intron
AGATTGGGCAGCCTGAGAGA+AGG 0.617228 8.2:+47726143 None:intergenic
TACAGAAAAGTGTCGAAAGT+GGG 0.617739 8.2:+47725623 None:intergenic
GGGCATGAGAGAACAGTGAG+AGG 0.621139 8.2:+47727662 None:intergenic
TAGAAAATTTGCTAACACAA+CGG 0.648999 8.2:+47725249 None:intergenic
TGGCAAATGTAATTCTGGTG+AGG 0.653371 8.2:+47725978 None:intergenic
AGAGAACAGTGAGAGGAAGG+CGG 0.655232 8.2:+47727669 None:intergenic
ATGATATCTACGTGAGACGA+GGG 0.656795 8.2:+47728018 None:intergenic
TCTCTGCGGTGATATCGTTG+TGG 0.661941 8.2:-47726254 MS.gene032296:CDS
GATGATATCTACGTGAGACG+AGG 0.662420 8.2:+47728017 None:intergenic
TCTTATAATTCAGAACGACA+AGG 0.672226 8.2:-47725567 MS.gene032296:intron
TAACGTGGATGTGAATTCTG+TGG 0.682540 8.2:-47726170 MS.gene032296:CDS
GAAACTAGATATTGGTAACG+TGG 0.690783 8.2:-47726185 MS.gene032296:CDS
AGTCATACAAGTAAGCATGT+GGG 0.710464 8.2:+47727813 None:intergenic
TGTAATGTACTCAGTCTCTG+CGG 0.744171 8.2:-47726268 MS.gene032296:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATTATATTATTAATTA+AGG - chr8.2:47725921-47725940 MS.gene032296:CDS 0.0%
!! ATATTCGAAAAATTTATAAT+AGG + chr8.2:47727538-47727557 None:intergenic 10.0%
!! ATCTATAACAATATTTAAAT+TGG + chr8.2:47726673-47726692 None:intergenic 10.0%
!! TTTAAATGAATTGTATAATT+GGG - chr8.2:47726617-47726636 MS.gene032296:intron 10.0%
!! ACATGTTAAGAAATTTAAAA+TGG - chr8.2:47726393-47726412 MS.gene032296:intron 15.0%
!! GTTTAAATGAATTGTATAAT+TGG - chr8.2:47726616-47726635 MS.gene032296:intron 15.0%
!! AAAGTCAAAATGAAGAAAAA+AGG + chr8.2:47726082-47726101 None:intergenic 20.0%
!! AAGTCAAAATGAAGAAAAAA+GGG + chr8.2:47726081-47726100 None:intergenic 20.0%
!! ACAATATTTAAATTGGAAAG+CGG + chr8.2:47726666-47726685 None:intergenic 20.0%
!! TAAAATGGTAAGTTTGTATT+TGG - chr8.2:47726408-47726427 MS.gene032296:intron 20.0%
!!! TTAAAGAAAATGGTTATTTC+TGG - chr8.2:47727919-47727938 MS.gene032296:CDS 20.0%
!!! TTTTTCGAATATACATTTTC+CGG - chr8.2:47727546-47727565 MS.gene032296:intron 20.0%
! AAAACGATGAAACTAGATAT+TGG - chr8.2:47727034-47727053 MS.gene032296:intron 25.0%
! GAAAATAAGAAAATACCTGA+TGG + chr8.2:47725602-47725621 None:intergenic 25.0%
! GTTATATTCAGTATTAATCG+CGG + chr8.2:47725739-47725758 None:intergenic 25.0%
! TACTAAACCAAATCATACAT+CGG + chr8.2:47726800-47726819 None:intergenic 25.0%
! TAGTTAAATCCCAGTATAAT+TGG + chr8.2:47726550-47726569 None:intergenic 25.0%
!!! AGTTTCATCGTTTTTAATGA+AGG + chr8.2:47727028-47727047 None:intergenic 25.0%
!!! TAGAAAATTTGCTAACACAA+CGG + chr8.2:47727981-47728000 None:intergenic 25.0%
!!! TGGACTTGTTTTAAAGAAAA+TGG - chr8.2:47727909-47727928 MS.gene032296:CDS 25.0%
AAATCCCAGTATAATTGGAA+GGG + chr8.2:47726545-47726564 None:intergenic 30.0%
ATACCCTTTAATGTGCATAT+TGG + chr8.2:47727842-47727861 None:intergenic 30.0%
ATACGTAAACAGATGAAGAA+GGG - chr8.2:47726819-47726838 MS.gene032296:intron 30.0%
ATCATTGGCAAATGTAATTC+TGG + chr8.2:47727257-47727276 None:intergenic 30.0%
ATTATAAGAGCTTGAAGCAT+AGG + chr8.2:47727649-47727668 None:intergenic 30.0%
TAAATCCCAGTATAATTGGA+AGG + chr8.2:47726546-47726565 None:intergenic 30.0%
TATACGTAAACAGATGAAGA+AGG - chr8.2:47726818-47726837 MS.gene032296:intron 30.0%
TCTAGTATTGCGATGTTATA+CGG - chr8.2:47727396-47727415 MS.gene032296:intron 30.0%
TCTTATAATTCAGAACGACA+AGG - chr8.2:47727660-47727679 MS.gene032296:CDS 30.0%
TGTAGTAAATGGTGAAATTC+CGG + chr8.2:47727568-47727587 None:intergenic 30.0%
TGTCCAATATGCACATTAAA+GGG - chr8.2:47727836-47727855 MS.gene032296:CDS 30.0%
TGTGAGTATAACTAAAGCTT+TGG - chr8.2:47727685-47727704 MS.gene032296:CDS 30.0%
TTATGCTCCTGAAAATGAAT+CGG - chr8.2:47727327-47727346 MS.gene032296:intron 30.0%
TTGTCCAATATGCACATTAA+AGG - chr8.2:47727835-47727854 MS.gene032296:CDS 30.0%
! AAATTTTCTAATATGCCGAG+GGG - chr8.2:47727991-47728010 MS.gene032296:CDS 30.0%
! CAAATTTTCTAATATGCCGA+GGG - chr8.2:47727990-47728009 MS.gene032296:CDS 30.0%
!! TGAGCTCTAAATGTAGTAAA+TGG + chr8.2:47727579-47727598 None:intergenic 30.0%
ACAAACCCTTCCAATTATAC+TGG - chr8.2:47726537-47726556 MS.gene032296:intron 35.0%
AGACAGTTAGGAACAGTATT+AGG + chr8.2:47727805-47727824 None:intergenic 35.0%
AGATTCATTTCCTCAAGAAG+CGG + chr8.2:47725508-47725527 None:intergenic 35.0%
AGTCATACAAGTAAGCATGT+GGG + chr8.2:47725417-47725436 None:intergenic 35.0%
CAAACCCTTCCAATTATACT+GGG - chr8.2:47726538-47726557 MS.gene032296:intron 35.0%
CAAGAATCCGATGTATGATT+TGG - chr8.2:47726790-47726809 MS.gene032296:intron 35.0%
GAGTGACATTACTTTCTAGA+AGG - chr8.2:47726716-47726735 MS.gene032296:intron 35.0%
GATACATCACCAAATGAAAG+TGG - chr8.2:47727766-47727785 MS.gene032296:CDS 35.0%
GATTAGAGATCTAGAGATCT+AGG + chr8.2:47726043-47726062 None:intergenic 35.0%
TACATTTGCCAATGATACTG+CGG - chr8.2:47727261-47727280 MS.gene032296:intron 35.0%
TGCAAACATAGTTGTCAAAG+CGG - chr8.2:47725806-47725825 MS.gene032296:intron 35.0%
TGTGGAATTAGCACAATCTT+CGG - chr8.2:47726991-47727010 MS.gene032296:intron 35.0%
TTGACAACTATGTTTGCAAC+AGG + chr8.2:47725804-47725823 None:intergenic 35.0%
! ACTTTCGACACTTTTCTGTA+CGG - chr8.2:47727607-47727626 MS.gene032296:intron 35.0%
! AGAACAGAGATGAGATTTTG+TGG - chr8.2:47726123-47726142 MS.gene032296:CDS 35.0%
! AGGCTAAGGTATTTGAGATT+GGG + chr8.2:47727232-47727251 None:intergenic 35.0%
! CTCTCTTTTGTTGCATGATT+TGG - chr8.2:47726897-47726916 MS.gene032296:intron 35.0%
! CTTTTTACATCTGAGGATCA+TGG - chr8.2:47725532-47725551 MS.gene032296:intron 35.0%
! GCAAATTTTCTAATATGCCG+AGG - chr8.2:47727989-47728008 MS.gene032296:CDS 35.0%
! TCTTGCTCTTTTTACATCTG+AGG - chr8.2:47725525-47725544 MS.gene032296:intron 35.0%
!! ATGCACATTAAAGGGTATCA+AGG - chr8.2:47727844-47727863 MS.gene032296:CDS 35.0%
!! GAAACTAGATATTGGTAACG+TGG - chr8.2:47727042-47727061 MS.gene032296:intron 35.0%
!! TACAGAAAAGTGTCGAAAGT+GGG + chr8.2:47727607-47727626 None:intergenic 35.0%
!!! GGTTATTTCTGGTGTTTTCT+TGG - chr8.2:47727930-47727949 MS.gene032296:CDS 35.0%
AAGTGAGACACAAGACAGTT+AGG + chr8.2:47727817-47727836 None:intergenic 40.0%
AGCTCCAATGTCATTCTCAA+CGG + chr8.2:47727191-47727210 None:intergenic 40.0%
ATCACCAAATGAAAGTGGCT+TGG - chr8.2:47727771-47727790 MS.gene032296:CDS 40.0%
ATGATATCTACGTGAGACGA+GGG + chr8.2:47725212-47725231 None:intergenic 40.0%
ATGCTTACTTGTATGACTCG+TGG - chr8.2:47725419-47725438 MS.gene032296:CDS 40.0%
ATGTAATTCTGGTGAGGCTA+AGG + chr8.2:47727246-47727265 None:intergenic 40.0%
ATTGCGATGTTATACGGCAA+AGG - chr8.2:47727402-47727421 MS.gene032296:intron 40.0%
CACGTAGATATCATCATCTC+TGG - chr8.2:47725218-47725237 MS.gene032296:CDS 40.0%
CGTAGTAGAAGTTGAATGAC+TGG + chr8.2:47725252-47725271 None:intergenic 40.0%
CTTGGACCAAAAGAAGAAGT+GGG - chr8.2:47727948-47727967 MS.gene032296:CDS 40.0%
CTTGGAGGAGAATGAGTAAA+GGG + chr8.2:47727357-47727376 None:intergenic 40.0%
GAGTCATACAAGTAAGCATG+TGG + chr8.2:47725418-47725437 None:intergenic 40.0%
GGTGAGTTTCAAACTCTTCA+GGG + chr8.2:47727170-47727189 None:intergenic 40.0%
GTTTAATTCCAGTGAGAGCT+TGG + chr8.2:47727375-47727394 None:intergenic 40.0%
TAACGTGGATGTGAATTCTG+TGG - chr8.2:47727057-47727076 MS.gene032296:intron 40.0%
TACGACTATAATCCCATCCT+CGG - chr8.2:47725268-47725287 MS.gene032296:CDS 40.0%
TCAAGGAGATTCTAGTGAGA+TGG - chr8.2:47727861-47727880 MS.gene032296:CDS 40.0%
TCTTGGACCAAAAGAAGAAG+TGG - chr8.2:47727947-47727966 MS.gene032296:CDS 40.0%
TGCTTACTTGTATGACTCGT+GGG - chr8.2:47725420-47725439 MS.gene032296:CDS 40.0%
TGGCAAATGTAATTCTGGTG+AGG + chr8.2:47727252-47727271 None:intergenic 40.0%
TGTAAGAAGAGACTGGTAGA+TGG - chr8.2:47727508-47727527 MS.gene032296:intron 40.0%
TGTAATGTACTCAGTCTCTG+CGG - chr8.2:47726959-47726978 MS.gene032296:intron 40.0%
TTCAAACTCTTCAGGGTAGA+TGG + chr8.2:47727163-47727182 None:intergenic 40.0%
TTTAATTCAGTATCCGACCC+AGG - chr8.2:47725712-47725731 MS.gene032296:CDS 40.0%
! ACTTCTCCCACTTCTTCTTT+TGG + chr8.2:47727957-47727976 None:intergenic 40.0%
! GAGGCTAAGGTATTTGAGAT+TGG + chr8.2:47727233-47727252 None:intergenic 40.0%
! GCTAGCTATGTTTTGCAGAA+TGG - chr8.2:47727889-47727908 MS.gene032296:CDS 40.0%
! GTTTGTTTGTTAGCGTAGCA+TGG - chr8.2:47726868-47726887 MS.gene032296:intron 40.0%
!! AGTTCCAGGGTTTCTAAGAT+TGG + chr8.2:47727103-47727122 None:intergenic 40.0%
!! GTACAGAAAAGTGTCGAAAG+TGG + chr8.2:47727608-47727627 None:intergenic 40.0%
!! GTTCCAGGGTTTCTAAGATT+GGG + chr8.2:47727102-47727121 None:intergenic 40.0%
!! TCATTTGGTGATGTATCCTG+CGG + chr8.2:47727763-47727782 None:intergenic 40.0%
AAGTAAGCATGTGGGTCTTC+GGG + chr8.2:47725409-47725428 None:intergenic 45.0%
ACCTGATGGAGAGTAGCTTA+GGG + chr8.2:47725588-47725607 None:intergenic 45.0%
ACGAAACTTGGCCAGAGATA+TGG + chr8.2:47727137-47727156 None:intergenic 45.0%
ATGAGAGAACAGTGAGAGGA+AGG + chr8.2:47725564-47725583 None:intergenic 45.0%
ATGTATCCTGCGGGCATATA+GGG + chr8.2:47727753-47727772 None:intergenic 45.0%
CAAGTAAGCATGTGGGTCTT+CGG + chr8.2:47725410-47725429 None:intergenic 45.0%
CGAAACTTGGCCAGAGATAT+GGG + chr8.2:47727136-47727155 None:intergenic 45.0%
CGACAGACATTTCCGTAAAC+CGG + chr8.2:47725459-47725478 None:intergenic 45.0%
CGGTGAGTTTCAAACTCTTC+AGG + chr8.2:47727171-47727190 None:intergenic 45.0%
CTCACCGTTGAGAATGACAT+TGG - chr8.2:47727184-47727203 MS.gene032296:intron 45.0%
GACAGACATTTCCGTAAACC+GGG + chr8.2:47725458-47725477 None:intergenic 45.0%
GATGATATCTACGTGAGACG+AGG + chr8.2:47725213-47725232 None:intergenic 45.0%
GCAACCTCTGTTGTCAATAG+CGG + chr8.2:47726365-47726384 None:intergenic 45.0%
GCTTGGAGGAGAATGAGTAA+AGG + chr8.2:47727358-47727377 None:intergenic 45.0%
GGTAGATGGAACACGAAACT+TGG + chr8.2:47727149-47727168 None:intergenic 45.0%
GTGTACGTGTAAGAAGAGAC+TGG - chr8.2:47727501-47727520 MS.gene032296:intron 45.0%
TAATTCCAGTGAGAGCTTGG+AGG + chr8.2:47727372-47727391 None:intergenic 45.0%
TACCTGATGGAGAGTAGCTT+AGG + chr8.2:47725589-47725608 None:intergenic 45.0%
TAGACCGCTATTGACAACAG+AGG - chr8.2:47726358-47726377 MS.gene032296:intron 45.0%
TAGTTGTCAAAGCGGAAACG+CGG - chr8.2:47725814-47725833 MS.gene032296:intron 45.0%
TCAGTATTAATCGCGGTCCT+GGG + chr8.2:47725732-47725751 None:intergenic 45.0%
TCTGTGGATATCCTTCTCTC+AGG - chr8.2:47727073-47727092 MS.gene032296:intron 45.0%
TTCAGTATTAATCGCGGTCC+TGG + chr8.2:47725733-47725752 None:intergenic 45.0%
TTGCCAATGATACTGCGGTT+GGG - chr8.2:47727266-47727285 MS.gene032296:intron 45.0%
TTTGCCAATGATACTGCGGT+TGG - chr8.2:47727265-47727284 MS.gene032296:intron 45.0%
! ACCAAATGAAAGTGGCTTGG+CGG - chr8.2:47727774-47727793 MS.gene032296:CDS 45.0%
! TGCCTTTTCTCCCATATCTC+TGG - chr8.2:47727123-47727142 MS.gene032296:intron 45.0%
!! CATTTGGTGATGTATCCTGC+GGG + chr8.2:47727762-47727781 None:intergenic 45.0%
!! GCGTTTGAAGTTTTCGGAGT+TGG + chr8.2:47725296-47725315 None:intergenic 45.0%
!! GGCGAAGCGTTTGAAGTTTT+CGG + chr8.2:47725302-47725321 None:intergenic 45.0%
!! GGGTCAACCGATTCATTTTC+AGG + chr8.2:47727337-47727356 None:intergenic 45.0%
!!! ATAACTTTTTAATAAATAAT+TGG - chr8.2:47726456-47726475 MS.gene032296:intron 5.0%
AATTGTCCCTATATGCCCGC+AGG - chr8.2:47727744-47727763 MS.gene032296:CDS 50.0%
ACGCATCTTACGAACGTCGA+TGG + chr8.2:47725359-47725378 None:intergenic 50.0%
AGAGAACAGTGAGAGGAAGG+CGG + chr8.2:47725561-47725580 None:intergenic 50.0%
ATTCTCCTCCAAGCTCTCAC+TGG - chr8.2:47727364-47727383 MS.gene032296:intron 50.0%
CGGAAATGTCTGTCGATGCT+TGG - chr8.2:47725464-47725483 MS.gene032296:CDS 50.0%
CGTAAGATGCGTCTCTCATG+TGG - chr8.2:47725367-47725386 MS.gene032296:CDS 50.0%
CTGCCCAATCTTAGAAACCC+TGG - chr8.2:47727096-47727115 MS.gene032296:intron 50.0%
GATCAACGCGGGCAAAAAAC+GGG + chr8.2:47726173-47726192 None:intergenic 50.0%
GATGTATCCTGCGGGCATAT+AGG + chr8.2:47727754-47727773 None:intergenic 50.0%
GGATCAACGCGGGCAAAAAA+CGG + chr8.2:47726174-47726193 None:intergenic 50.0%
GGCCAGAGATATGGGAGAAA+AGG + chr8.2:47727128-47727147 None:intergenic 50.0%
GGGAGAAAAGGCAAGTTCCA+GGG + chr8.2:47727116-47727135 None:intergenic 50.0%
TATTAATCGCGGTCCTGGGT+CGG + chr8.2:47725728-47725747 None:intergenic 50.0%
TCTCTGCGGTGATATCGTTG+TGG - chr8.2:47726973-47726992 MS.gene032296:intron 50.0%
TGCCAATGATACTGCGGTTG+GGG - chr8.2:47727267-47727286 MS.gene032296:intron 50.0%
TGGGAGAAAAGGCAAGTTCC+AGG + chr8.2:47727117-47727136 None:intergenic 50.0%
TTGTCAATAGCGGTCTAGCG+CGG + chr8.2:47726355-47726374 None:intergenic 50.0%
! ACCGCCAAGCCACTTTCATT+TGG + chr8.2:47727778-47727797 None:intergenic 50.0%
!! GAGCACAGCGTTGACGAAAA+CGG + chr8.2:47725323-47725342 None:intergenic 50.0%
AGATTGGGCAGCCTGAGAGA+AGG + chr8.2:47727087-47727106 None:intergenic 55.0%
AGGCAAAAAGCGGGTCAACG+CGG + chr8.2:47726206-47726225 None:intergenic 55.0%
AGTCGCGCGTTAGGCAAAAG+CGG + chr8.2:47726278-47726297 None:intergenic 55.0%
CATTTCCGTAAACCGGGCTC+CGG + chr8.2:47725452-47725471 None:intergenic 55.0%
GCCCTAAGCTACTCTCCATC+AGG - chr8.2:47725584-47725603 MS.gene032296:CDS 55.0%
GGCAAAAAGCGGATCAACGC+GGG + chr8.2:47726184-47726203 None:intergenic 55.0%
GGCCACCCCAAAAATTGACG+CGG - chr8.2:47725845-47725864 MS.gene032296:CDS 55.0%
GGGCAAAAAGCGGATCAACG+CGG + chr8.2:47726185-47726204 None:intergenic 55.0%
GGGCATGAGAGAACAGTGAG+AGG + chr8.2:47725568-47725587 None:intergenic 55.0%
GGGTCAACGCAGGCAAAAAG+CGG + chr8.2:47726216-47726235 None:intergenic 55.0%
GGTCAACGCAGGCAAAAAGC+GGG + chr8.2:47726215-47726234 None:intergenic 55.0%
GTCCCCAACCGCAGTATCAT+TGG + chr8.2:47727272-47727291 None:intergenic 55.0%
TAGGCAAAAGCGGGTCAACG+CGG + chr8.2:47726268-47726287 None:intergenic 55.0%
TATGACTCGTGGGTCCGATC+CGG - chr8.2:47725430-47725449 MS.gene032296:CDS 55.0%
TTCCTCAAGAAGCGGACCAG+CGG + chr8.2:47725500-47725519 None:intergenic 55.0%
! ACGGCGCCGCGTCAATTTTT+GGG + chr8.2:47725854-47725873 None:intergenic 55.0%
! CTTTTGCCTAACGCGCGACT+CGG - chr8.2:47726278-47726297 MS.gene032296:intron 55.0%
! TCCGCGTCATGTAAAAGCGC+CGG + chr8.2:47725891-47725910 None:intergenic 55.0%
AGGCAAAAGCGGGTCAACGC+GGG + chr8.2:47726267-47726286 None:intergenic 60.0%
GAAGCGGACCAGCGGCAATA+CGG + chr8.2:47725492-47725511 None:intergenic 60.0%
GCTTGGATCCGTATTGCCGC+TGG - chr8.2:47725481-47725500 MS.gene032296:intron 60.0%
GGCAAAAAGCGGGTCAACGC+AGG + chr8.2:47726205-47726224 None:intergenic 60.0%
GGCAAAAAGCGGGTCAACGC+GGG + chr8.2:47726205-47726224 None:intergenic 60.0%
GGGTCAACGCGGGCAAAAAG+CGG + chr8.2:47726195-47726214 None:intergenic 60.0%
GTCGCGCGTTAGGCAAAAGC+GGG + chr8.2:47726277-47726296 None:intergenic 60.0%
! AGCTTTGGTGTGTGCGTGCG+TGG - chr8.2:47727700-47727719 MS.gene032296:CDS 60.0%
! AGTTTTCGGAGTTGGCGCCG+AGG + chr8.2:47725288-47725307 None:intergenic 60.0%
! CGGCGCCGCGTCAATTTTTG+GGG + chr8.2:47725853-47725872 None:intergenic 60.0%
! GACGGCGCCGCGTCAATTTT+TGG + chr8.2:47725855-47725874 None:intergenic 60.0%
! GCCGGCGCTTTTACATGACG+CGG - chr8.2:47725887-47725906 MS.gene032296:CDS 60.0%
!! CGCCGCGTCAATTTTTGGGG+TGG + chr8.2:47725850-47725869 None:intergenic 60.0%
AATTGACGCGGCGCCGTCAG+CGG - chr8.2:47725857-47725876 MS.gene032296:CDS 65.0%
CCGATCCGGAGCCCGGTTTA+CGG - chr8.2:47725444-47725463 MS.gene032296:CDS 65.0%
CCGTAAACCGGGCTCCGGAT+CGG + chr8.2:47725447-47725466 None:intergenic 65.0%
CGGGTCAACGCGGGCAAAAG+CGG + chr8.2:47726258-47726277 None:intergenic 65.0%
GGGCAAAAGCGGGTCAACGC+AGG + chr8.2:47726247-47726266 None:intergenic 65.0%
GGGTCAACGCGGGCAAAAGC+GGG + chr8.2:47726257-47726276 None:intergenic 65.0%
GTCAAAGCGGAAACGCGGCG+CGG - chr8.2:47725819-47725838 MS.gene032296:intron 65.0%
TGCCGCTGGTCCGCTTCTTG+AGG - chr8.2:47725495-47725514 MS.gene032296:intron 65.0%
TGGCAGACAGCAGAGCCCCT+CGG + chr8.2:47728009-47728028 None:intergenic 65.0%
! TCGGAGTTGGCGCCGAGGAT+GGG + chr8.2:47725283-47725302 None:intergenic 65.0%
! TTCGGAGTTGGCGCCGAGGA+TGG + chr8.2:47725284-47725303 None:intergenic 65.0%
ACGCGACCGAGTCGCGCGTT+AGG + chr8.2:47726287-47726306 None:intergenic 70.0%
ATTGACGCGGCGCCGTCAGC+GGG - chr8.2:47725858-47725877 MS.gene032296:CDS 70.0%
CGGTCGCGTTCTGACGCGCT+AGG - chr8.2:47726298-47726317 MS.gene032296:intron 70.0%
CGTGGGTCCGATCCGGAGCC+CGG - chr8.2:47725437-47725456 MS.gene032296:CDS 75.0%
GCCGGCGTATCGCCCGCTGA+CGG + chr8.2:47725873-47725892 None:intergenic 75.0%
GCCGTCAGCGGGCGATACGC+CGG - chr8.2:47725869-47725888 MS.gene032296:CDS 75.0%
GGTCTAGCGCGGCGCGATCG+CGG + chr8.2:47726344-47726363 None:intergenic 75.0%
! AGCGGAAACGCGGCGCGGCG+CGG - chr8.2:47725824-47725843 MS.gene032296:intron 80.0%
! AGCGCGGCGCGATCGCGGCG+CGG + chr8.2:47726339-47726358 None:intergenic 85.0%
! GGCGCGATCGCGGCGCGGAG+TGG + chr8.2:47726334-47726353 None:intergenic 85.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 47725205 47728044 47725205 ID=MS.gene032296
chr8.2 mRNA 47725205 47728044 47725205 ID=MS.gene032296.t1;Parent=MS.gene032296
chr8.2 exon 47727644 47728044 47727644 ID=MS.gene032296.t1.exon1;Parent=MS.gene032296.t1
chr8.2 CDS 47727644 47728044 47727644 ID=cds.MS.gene032296.t1;Parent=MS.gene032296.t1
chr8.2 exon 47725826 47726276 47725826 ID=MS.gene032296.t1.exon2;Parent=MS.gene032296.t1
chr8.2 CDS 47725826 47726276 47725826 ID=cds.MS.gene032296.t1;Parent=MS.gene032296.t1
chr8.2 exon 47725568 47725732 47725568 ID=MS.gene032296.t1.exon3;Parent=MS.gene032296.t1
chr8.2 CDS 47725568 47725732 47725568 ID=cds.MS.gene032296.t1;Parent=MS.gene032296.t1
chr8.2 exon 47725205 47725483 47725205 ID=MS.gene032296.t1.exon4;Parent=MS.gene032296.t1
chr8.2 CDS 47725205 47725483 47725205 ID=cds.MS.gene032296.t1;Parent=MS.gene032296.t1
Gene Sequence

>MS.gene032296

ATGACCCTCGTCTCACGTAGATATCATCATCTCTGGAAGCATCTCCAGTCATTCAACTTCTACTACGACTATAATCCCATCCTCGGCGCCAACTCCGAAAACTTCAAACGCTTCGCCGTTTTCGTCAACGCTGTGCTCACTCAACGACATTCCATCGACGTTCGTAAGATGCGTCTCTCATGTGGCTACTTCAAGTCTCAACCCGAAGACCCACATGCTTACTTGTATGACTCGTGGGTCCGATCCGGAGCCCGGTTTACGGAAATGTCTGTCGATGCTTGGATCCGTATTGCCGCTGGTCCGCTTCTTGAGGAAATGAATCTTGCTCTTTTTACATCTGAGGATCATGGCTTCCGCCTTCCTCTCACTGTTCTCTCATGCCCTAAGCTACTCTCCATCAGGTATTTTCTTATTTTCTAATTATAGATTTTACTTAAAATATGTACATTGTTCTTATTGATTAAAATGTGATATGATAGATGATCAAATTTCACACAAAAAATTGTTTTTAATTCAGTATCCGACCCAGGACCGCGATTAATACTGAATATAACATATGATCAAATTAAGATTTAACTGATACTGATCATACTGATCCTGTTGCAAACATAGTTGTCAAAGCGGAAACGCGGCGCGGCGCGGCCACCCCAAAAATTGACGCGGCGCCGTCAGCGGGCGATACGCCGGCGCTTTTACATGACGCGGACACTAAAACTAAATATTATATTATTAATTAAGGACTAAAAAAGTATGAAAAAAATATATTTATTATTAATATAGATGAGAAAATTTTATTTTGCATTCACATTTAGACTTCATCTTGATCATATTAATTCCTAGATCTCTAGATCTCTAATCAATCTTTCACTTCTTCCCTTTTTTCTTCATTTTGACTTTAAAAAAAATGATGATTAACAGAGAACAGAGATGAGATTTTGTGGTTTCTGATGAGATTTTTTTGTTGACCCGTTTTTTGCCCGCGTTGATCCGCTTTTTGCCCGCGTTGACCCGCTTTTTGCCTGCGTTGACCCGCTTTTTGCCTGCGTTGACCCGCTTTTGCCCGCGTTGACCCGCTTTTGCCTAACGCGCGACTCGGTCGCGTTCTGACGCGCTAGGCTGTTTTCAGCCACTCCGCGCCGCGATCGCGCCGCGCTAGACCGCTATTGACAACAGAGGTTGCAAATTTGCACATGTTAAGAAATTTAAAATGGTAAGTTTGTATTTGGAACTTGTACATTTTATATTAAATAGATAACTTTTTAATAAATAATTGGTGTTTTTCAATAAGAATACTTTTAGTTGCTTTAACACGTGCAATATTGCACATTTTAGACAAACCCTTCCAATTATACTGGGATTTAACTATTTTTTTTTTTTTTTTTATATAAAAAGATAAATTCTAATTACAATAGTTTAAATGAATTGTATAATTGGGAGAATGAAAAAGCTAAGACAAATCCGCTTTCCAATTTAAATATTGTTATAGATTATAGATATAGATGATGATTGATGAGTGACATTACTTTCTAGAAGGATCTATTCTATCTTGTCGTGACGAGTCATTATACTGTTATGTTTCTGTTAGCAAGAATCCGATGTATGATTTGGTTTAGTATACGTAAACAGATGAAGAAGGGAATAATATACAAGATTATTGAAGAACGTTTGTTTGTTAGCGTAGCATGGTTATCTCTCTCTTTTGTTGCATGATTTGGTTTCATCTTATTGCACTAAGAAACTTATAATAACATTTATGTAATGTACTCAGTCTCTGCGGTGATATCGTTGTGGAATTAGCACAATCTTCGGCAATTTCTTTGCCTTCATTAAAAACGATGAAACTAGATATTGGTAACGTGGATGTGAATTCTGTGGATATCCTTCTCTCAGGCTGCCCAATCTTAGAAACCCTGGAACTTGCCTTTTCTCCCATATCTCTGGCCAAGTTTCGTGTTCCATCTACCCTGAAGAGTTTGAAACTCACCGTTGAGAATGACATTGGAGCTTGTGTTGAGATTGATGCACCCAATCTCAAATACCTTAGCCTCACCAGAATTACATTTGCCAATGATACTGCGGTTGGGGACTTGCACAATGTGAAAGAAGCATATCTTGATGTATTTTATGCTCCTGAAAATGAATCGGTTGACCCTTTACTCATTCTCCTCCAAGCTCTCACTGGAATTAAACGTCTAGTATTGCGATGTTATACGGCAAAGGTGAGTGAAATTTTCTTTATCTATGTTTTCATTATATATTTTGTAGTTTTGTGCTAATATTACTTACTGTTGTTGTTGTGTACGTGTAAGAAGAGACTGGTAGATGGTCAACCTATTATAAATTTTTCGAATATACATTTTCCGGAATTTCACCATTTACTACATTTAGAGCTCATTCTTCCCACTTTCGACACTTTTCTGTACGGTGTACTTCAGAAATGTCCTATGCTTCAAGCTCTTATAATTCAGAACGACAAGGTTTGTGAGTATAACTAAAGCTTTGGTGTGTGCGTGCGTGGTTTTTGTTTTCATCTTACGTCAATTGTCCCTATATGCCCGCAGGATACATCACCAAATGAAAGTGGCTTGGCGGTGAAGCCTAATACTGTTCCTAACTGTCTTGTGTCTCACTTGTCCAATATGCACATTAAAGGGTATCAAGGAGATTCTAGTGAGATGGAATTTGCTAGCTATGTTTTGCAGAATGGACTTGTTTTAAAGAAAATGGTTATTTCTGGTGTTTTCTTGGACCAAAAGAAGAAGTGGGAGAAGTACCGTTGTGTTAGCAAATTTTCTAATATGCCGAGGGGCTCTGCTGTCTGCCAAGTTACATTTGACTGA
Protein sequence

>MS.gene032296.t1

MTLVSRRYHHLWKHLQSFNFYYDYNPILGANSENFKRFAVFVNAVLTQRHSIDVRKMRLSCGYFKSQPEDPHAYLYDSWVRSGARFTEMSVDAWIRIAAGPLLEEMNLALFTSEDHGFRLPLTVLSCPKLLSISLCGDIVVELAQSSAISLPSLKTMKLDIGNVDVNSVDILLSGCPILETLELAFSPISLAKFRVPSTLKSLKLTVENDIGACVEIDAPNLKYLSLTRITFANDTAVGDLHNVKEAYLDVFYAPENESVDPLLILLQALTGIKRLVLRCYTAKRLVDGQPIINFSNIHFPEFHHLLHLELILPTFDTFLYGVLQKCPMLQALIIQNDKDTSPNESGLAVKPNTVPNCLVSHLSNMHIKGYQGDSSEMEFASYVLQNGLVLKKMVISGVFLDQKKKWEKYRCVSKFSNMPRGSAVCQVTFD