AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25411

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25411.t1 MTR_8g468810 85.455 330 45 2 1 329 51 378 0.0 560
MS.gene25411.t1 MTR_8g468810 86.728 324 40 2 1 323 51 372 0.0 557
MS.gene25411.t1 MTR_8g468810 86.891 267 33 1 1 267 51 315 2.49e-164 461
MS.gene25411.t1 MTR_8g468810 86.891 267 33 1 1 267 51 315 6.10e-164 461
MS.gene25411.t1 MTR_8g468810 86.891 267 33 1 1 267 51 315 7.45e-164 460
MS.gene25411.t1 MTR_8g058970 65.217 345 101 3 1 326 45 389 3.12e-146 421
MS.gene25411.t1 MTR_2g083840 54.711 329 139 6 1 326 47 368 2.43e-105 316
MS.gene25411.t1 MTR_4g032730 52.727 330 138 8 3 326 43 360 1.67e-96 294
MS.gene25411.t1 MTR_8g062270 50.462 325 145 9 1 317 41 357 2.01e-92 283
MS.gene25411.t1 MTR_8g468840 50.909 330 153 5 2 327 40 364 2.15e-92 283
MS.gene25411.t1 MTR_5g092960 51.045 335 141 10 3 323 47 372 2.20e-85 263
MS.gene25411.t1 MTR_8g468760 51.235 324 111 10 1 316 34 318 6.21e-85 263
MS.gene25411.t1 MTR_8g467800 50.459 327 143 8 2 322 46 359 7.36e-85 263
MS.gene25411.t1 MTR_5g092960 50.746 335 142 10 3 323 47 372 4.05e-84 262
MS.gene25411.t1 MTR_8g467800 52.399 271 117 5 2 267 46 309 1.66e-73 231
MS.gene25411.t1 MTR_8g445780 43.963 323 163 7 4 322 41 349 2.63e-73 232
MS.gene25411.t1 MTR_8g014260 43.544 333 169 10 4 326 44 367 1.49e-72 232
MS.gene25411.t1 MTR_4g050250 40.845 355 177 8 1 331 47 392 2.49e-62 204
MS.gene25411.t1 MTR_8g469020 38.529 340 178 10 2 322 34 361 5.82e-59 196
MS.gene25411.t1 MTR_8g058965 41.159 345 174 11 2 328 40 373 3.62e-58 194
MS.gene25411.t1 MTR_8g070950 37.427 342 156 13 1 322 41 344 3.08e-50 173
MS.gene25411.t1 MTR_8g014160 37.624 303 169 8 2 287 67 366 8.93e-50 171
MS.gene25411.t1 MTR_8g062010 37.647 340 185 11 1 334 35 353 2.04e-44 157
MS.gene25411.t1 MTR_8g062080 37.538 333 171 13 1 322 49 355 1.24e-42 153
MS.gene25411.t1 MTR_8g062310 37.072 321 164 12 4 322 54 338 4.07e-41 149
MS.gene25411.t1 MTR_8g077460 43.523 193 103 4 4 191 38 229 6.91e-39 142
MS.gene25411.t1 MTR_8g467790 33.908 348 150 14 1 326 174 463 1.90e-37 140
MS.gene25411.t1 MTR_8g062300 34.722 360 181 11 1 326 52 391 1.20e-36 137
MS.gene25411.t1 MTR_4g024710 42.723 213 113 5 117 326 58 264 1.20e-34 130
MS.gene25411.t1 MTR_4g024745 40.376 213 118 5 117 326 12 218 4.14e-29 113
MS.gene25411.t1 MTR_5g021550 52.212 113 51 3 4 113 47 159 1.09e-28 108
MS.gene25411.t1 MTR_8g014290 44.715 123 59 5 4 117 38 160 6.91e-25 99.0
MS.gene25411.t1 MTR_2g086020 41.875 160 74 6 1 153 1 148 3.12e-23 98.6
MS.gene25411.t1 MTR_8g014210 42.857 154 83 4 174 327 1 149 3.79e-21 90.9
MS.gene25411.t1 MTR_4g113810 33.143 175 100 6 57 221 1 168 9.62e-20 85.5
MS.gene25411.t1 MTR_8g014220 42.742 124 59 5 43 161 44 160 3.18e-18 80.9
MS.gene25411.t1 MTR_4g024720 38.168 131 58 6 4 113 45 173 3.00e-17 79.3
MS.gene25411.t1 MTR_4g056070 30.719 306 179 13 41 330 109 397 4.11e-17 82.0
MS.gene25411.t1 MTR_4g056040 30.573 314 178 13 41 334 99 392 3.65e-16 79.7
MS.gene25411.t1 MTR_1g041245 34.884 172 89 6 2 166 37 192 3.94e-15 75.9
MS.gene25411.t1 MTR_4g056030 30.855 269 154 12 41 294 69 320 4.21e-14 73.2
MS.gene25411.t1 MTR_4g055990 29.932 294 174 12 41 318 106 383 7.42e-14 72.8
MS.gene25411.t1 MTR_4g055990 29.932 294 174 12 41 318 106 383 7.95e-14 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25411.t1 AT1G55660 28.440 218 140 8 2 212 79 287 5.37e-15 75.5
MS.gene25411.t1 AT1G55660 28.440 218 140 8 2 212 79 287 8.87e-15 75.5
MS.gene25411.t1 AT4G14103 27.869 244 152 9 2 234 34 264 1.22e-13 71.6
MS.gene25411.t1 AT4G14103 27.869 244 152 9 2 234 34 264 1.22e-13 71.6
MS.gene25411.t1 AT4G14103 27.869 244 152 9 2 234 34 264 1.23e-13 72.0
MS.gene25411.t1 AT5G56370 27.444 266 162 10 2 261 28 268 1.79e-13 70.5
MS.gene25411.t1 AT5G56370 27.444 266 162 10 2 261 28 268 3.32e-13 70.5
MS.gene25411.t1 AT5G56370 27.444 266 162 10 2 261 28 268 3.32e-13 70.5
MS.gene25411.t1 AT4G26340 27.160 324 196 13 2 316 28 320 3.45e-13 70.5
MS.gene25411.t1 AT4G00315 29.091 220 136 9 2 212 28 236 1.83e-12 68.2
MS.gene25411.t1 AT1G05080 24.058 345 220 14 3 333 40 356 8.32e-12 66.2
MS.gene25411.t1 AT4G09920 26.038 265 171 11 2 259 28 274 3.27e-11 63.9
MS.gene25411.t1 AT4G09920 26.038 265 171 11 2 259 28 274 3.27e-11 63.9
MS.gene25411.t1 AT3G50710 24.924 329 204 12 3 318 29 327 8.13e-11 63.2
MS.gene25411.t1 AT4G09920 26.038 265 171 11 2 259 28 274 8.72e-11 62.4
MS.gene25411.t1 AT4G09920 26.038 265 171 11 2 259 28 274 8.72e-11 62.4
MS.gene25411.t1 AT4G09920 26.038 265 171 11 2 259 28 274 8.72e-11 62.4

Find 83 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGCAATGCAACTTCTAT+TGG 0.243149 8.2:+40413395 MS.gene25411:CDS
AGTTCCAAAGTTTCTAAGAT+TGG 0.318329 8.2:-40413230 None:intergenic
TCTCTTCTCCTCCGAGGATC+AGG 0.325831 8.2:+40412364 MS.gene25411:CDS
TGCAGGTTATATCACCATTT+CGG 0.340552 8.2:+40413907 MS.gene25411:intron
GAGGACGGCATTGACGAATA+TGG 0.341114 8.2:-40412204 None:intergenic
TCGGCCTCGCCGTCGACATC+GGG 0.348507 8.2:-40412164 None:intergenic
TTGGCAATGCAACTTCTATT+GGG 0.363130 8.2:+40413396 MS.gene25411:CDS
TGCATTGCCAAATGTGATAT+TGG 0.374879 8.2:-40413384 None:intergenic
CGGTGAGATCATTGTGGAAT+TGG 0.382138 8.2:+40413109 MS.gene25411:CDS
TGTGGAATTGGCACAATCTT+CGG 0.386985 8.2:+40413121 MS.gene25411:CDS
CCGAATCTTTGGCCAAGCTT+AGG 0.388638 8.2:+40413264 MS.gene25411:CDS
GGCAGAGAGAAGGATATTAA+TGG 0.390004 8.2:-40413204 None:intergenic
CGAATCTTTGGCCAAGCTTA+GGG 0.393635 8.2:+40413265 MS.gene25411:CDS
AGCCTCACCAATATCACATT+TGG 0.403388 8.2:+40413377 MS.gene25411:CDS
TTCGGCCTCGCCGTCGACAT+CGG 0.410886 8.2:-40412165 None:intergenic
CGTGGATCCGCACCGCTGTT+GGG 0.414380 8.2:+40412315 MS.gene25411:CDS
GGCAAAGTCTTTGAAATGTT+CGG 0.419126 8.2:-40412183 None:intergenic
CGAAGTTATCAAGTTGAGAT+TGG 0.421124 8.2:-40412274 None:intergenic
CTCACAGGTATCTTCATCTC+TGG 0.428747 8.2:+40412102 MS.gene25411:CDS
ACTCTCCTCCAAGCTCTCTC+TGG 0.432658 8.2:+40413494 MS.gene25411:CDS
CCTAAGCTTGGCCAAAGATT+CGG 0.439078 8.2:-40413264 None:intergenic
ACGTGGATCCGCACCGCTGT+TGG 0.441351 8.2:+40412314 MS.gene25411:CDS
GGCCAAAGATTCGGTTGAAA+AGG 0.442677 8.2:-40413255 None:intergenic
TACGAAGGACCCGCGACATT+CGG 0.443329 8.2:+40412231 MS.gene25411:CDS
TCCTCTGTTCTATACCGAAA+TGG 0.446187 8.2:-40413921 None:intergenic
TGAAGAACGTCGTACAGAAA+AGG 0.449207 8.2:-40413760 None:intergenic
CTGTCCAATCTTAGAAACTT+TGG 0.459587 8.2:+40413226 MS.gene25411:CDS
CTTGGAGGAGAGTGAGTAAA+GGG 0.461485 8.2:-40413484 None:intergenic
GCACTAACCTCTTCTTCATC+AGG 0.465056 8.2:+40412417 MS.gene25411:CDS
ATTATGAGAGCTTGAAGTAT+AGG 0.476673 8.2:-40413790 None:intergenic
CTCTTCTCCTCCGAGGATCA+GGG 0.489308 8.2:+40412365 MS.gene25411:CDS
CATCGTAGAAATCAAAAGCT+TGG 0.496050 8.2:-40412133 None:intergenic
GAAGAACGTCGTACAGAAAA+GGG 0.498849 8.2:-40413759 None:intergenic
CAAAGTTTCTAAGATTGGAC+AGG 0.500334 8.2:-40413225 None:intergenic
ATGTGATATTGGTGAGGCTA+AGG 0.505330 8.2:-40413373 None:intergenic
CAATGAGTCTTGTTTCTCAC+AGG 0.521437 8.2:+40412087 None:intergenic
ATTAAAAGTCTAGTGTTTCG+TGG 0.521993 8.2:+40413518 MS.gene25411:CDS
TACAGAAAAGGGTCGAAAGT+AGG 0.524208 8.2:-40413748 None:intergenic
ACAGAAAAGGGTCGAAAGTA+GGG 0.524425 8.2:-40413747 None:intergenic
CGCTGTTGGGCCCCACCTTG+AGG 0.527400 8.2:+40412328 MS.gene25411:CDS
TAATGCCAGAGAGAGCTTGG+AGG 0.531658 8.2:-40413499 None:intergenic
GCTTGGAGGAGAGTGAGTAA+AGG 0.536107 8.2:-40413485 None:intergenic
AGATGTAATCTCTTCAATGA+TGG 0.541081 8.2:-40413155 None:intergenic
ACGTACCAAAAGAGATTACT+TGG 0.545584 8.2:+40413649 MS.gene25411:intron
ACGCATCTTCCGAATGTCGC+GGG 0.555891 8.2:-40412240 None:intergenic
GAGTTTGAAGCCCTGATCCT+CGG 0.565083 8.2:-40412375 None:intergenic
CTCAAGGTGGGGCCCAACAG+CGG 0.568619 8.2:-40412327 None:intergenic
AACAGATTCACTTTCAGAAG+CGG 0.572108 8.2:-40413459 None:intergenic
GAATACTACCCCGATGTCGA+CGG 0.574728 8.2:+40412155 MS.gene25411:CDS
AGATTGGACAGGCAGAGAGA+AGG 0.579911 8.2:-40413214 None:intergenic
TGTAATGTACACAGTCTCTA+CGG 0.579931 8.2:+40413089 MS.gene25411:intron
GGAAGATGGAACCCTAAGCT+TGG 0.579963 8.2:-40413276 None:intergenic
GTTTCGTGGTTATACAGCAA+AGG 0.594947 8.2:+40413532 MS.gene25411:CDS
TGCATGATAGCACCGCGAGT+GGG 0.596169 8.2:-40412397 None:intergenic
ACATCTAGACACTGGTAATG+TGG 0.599292 8.2:+40413172 MS.gene25411:CDS
ACGGTTACCTGATGAAGAAG+AGG 0.599819 8.2:-40412424 None:intergenic
CTGAACGCTCTGTTGACACG+TGG 0.601980 8.2:+40412297 MS.gene25411:CDS
GAGATGAAGATCCTCAAGGT+GGG 0.607880 8.2:-40412339 None:intergenic
CGGAAGATGCGTCTCTCTTG+CGG 0.610368 8.2:+40412251 MS.gene25411:CDS
TATTGGGAACTTGCACAATG+TGG 0.620226 8.2:+40413412 MS.gene25411:CDS
AGGCTAAGGTACTTGAGACC+AGG 0.622331 8.2:-40413359 None:intergenic
GGGCTTCAAACTCCCACTCG+CGG 0.625160 8.2:+40412385 MS.gene25411:CDS
CGCGGGTCCTTCGTAGAGAG+AGG 0.630192 8.2:-40412223 None:intergenic
AGATGAAGATCCTCAAGGTG+GGG 0.631714 8.2:-40412338 None:intergenic
TGGGAACTTGCACAATGTGG+AGG 0.632693 8.2:+40413415 MS.gene25411:CDS
GACGCATCTTCCGAATGTCG+CGG 0.638940 8.2:-40412241 None:intergenic
CGAGATGAAGATCCTCAAGG+TGG 0.647298 8.2:-40412340 None:intergenic
GGTCCTTCGTAGAGAGAGGA+CGG 0.648635 8.2:-40412219 None:intergenic
GAGCGAGATGAAGATCCTCA+AGG 0.648810 8.2:-40412343 None:intergenic
TGTCTTGAGATAAACACACC+TGG 0.659546 8.2:+40413341 MS.gene25411:CDS
ATGCCGTCCTCTCTCTACGA+AGG 0.660874 8.2:+40412216 MS.gene25411:CDS
AAGAGATTACATCTAGACAC+TGG 0.663342 8.2:+40413164 MS.gene25411:CDS
GTGCATGATAGCACCGCGAG+TGG 0.666468 8.2:-40412398 None:intergenic
TCTCTACGGTGAGATCATTG+TGG 0.674088 8.2:+40413103 MS.gene25411:CDS
TCTCGCTCTCTTCTCCTCCG+AGG 0.674729 8.2:+40412358 MS.gene25411:CDS
GGTGGGGCCCAACAGCGGTG+CGG 0.681048 8.2:-40412322 None:intergenic
TGCCAAATGTGATATTGGTG+AGG 0.688345 8.2:-40413379 None:intergenic
ACCATTTCGGTATAGAACAG+AGG 0.688853 8.2:+40413920 MS.gene25411:CDS
TTTGAAGCCCTGATCCTCGG+AGG 0.698203 8.2:-40412372 None:intergenic
CGGCCTCGCCGTCGACATCG+GGG 0.702901 8.2:-40412163 None:intergenic
TCTCATAATTCAGTATGACA+AGG 0.711322 8.2:+40413804 MS.gene25411:CDS
CTACCCCGATGTCGACGGCG+AGG 0.714394 8.2:+40412160 MS.gene25411:CDS
GCTCTAGATGTAGTAAACAG+CGG 0.731159 8.2:-40413717 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATAATTAGTAAACAAGA+AGG + chr8.2:40412964-40412983 MS.gene25411:intron 15.0%
!!! AATGCTTTTTAAAAAAGTTT+AGG - chr8.2:40412743-40412762 None:intergenic 15.0%
!!! AGTAAATTTAAAACTTTTCA+AGG - chr8.2:40413300-40413319 None:intergenic 15.0%
!!! TTTATTTGTTACAGAAAAAT+AGG + chr8.2:40412499-40412518 MS.gene25411:intron 15.0%
!! AATGGAATATATAATACACA+AGG - chr8.2:40413577-40413596 None:intergenic 20.0%
!! CAAATATGAAGGAAAAAAAA+CGG - chr8.2:40412446-40412465 None:intergenic 20.0%
!! CACAAAACTATTACATATAA+TGG - chr8.2:40413595-40413614 None:intergenic 20.0%
!! TACAGAAAAATAGGTATAAT+GGG + chr8.2:40412508-40412527 MS.gene25411:intron 20.0%
!! TATTAACTAAAAAGTGAGTA+GGG + chr8.2:40412933-40412952 MS.gene25411:intron 20.0%
!! TGTTATATGAAAATAACTCA+AGG + chr8.2:40412775-40412794 MS.gene25411:intron 20.0%
!! TTACAGAAAAATAGGTATAA+TGG + chr8.2:40412507-40412526 MS.gene25411:intron 20.0%
!! TTATTAACTAAAAAGTGAGT+AGG + chr8.2:40412932-40412951 MS.gene25411:intron 20.0%
!!! ATTTTCGAAAAATCTATAGA+AGG - chr8.2:40413682-40413701 None:intergenic 20.0%
!!! TGTGTTTTTGTTTGATTATT+TGG + chr8.2:40413865-40413884 MS.gene25411:intron 20.0%
! AAAATAACTCAAGGAAATCT+AGG + chr8.2:40412784-40412803 MS.gene25411:intron 25.0%
! ATTAACTAAAAAGTGAGTAG+GGG + chr8.2:40412934-40412953 MS.gene25411:intron 25.0%
! CTGTTATTACTCAAATATGA+AGG - chr8.2:40412457-40412476 None:intergenic 25.0%
! TAAAAAAGTTTAGGTGAGTT+GGG - chr8.2:40412734-40412753 None:intergenic 25.0%
! TGATATGTAAGTACATTTGT+TGG + chr8.2:40412616-40412635 MS.gene25411:intron 25.0%
! TTAAAAAAGTTTAGGTGAGT+TGG - chr8.2:40412735-40412754 None:intergenic 25.0%
!!! AGTTGATAAGTACATTTGTT+GGG + chr8.2:40412667-40412686 MS.gene25411:intron 25.0%
!!! TTAAAACTTTTCAAGGAAGA+TGG - chr8.2:40413293-40413312 None:intergenic 25.0%
!!! TTTTTCGAAAATGCACTTTT+CGG + chr8.2:40413690-40413709 MS.gene25411:CDS 25.0%
ACAATGAAATTTGACTCAGT+TGG + chr8.2:40412585-40412604 MS.gene25411:intron 30.0%
AGATGTAATCTCTTCAATGA+TGG - chr8.2:40413158-40413177 None:intergenic 30.0%
ATTAAAAGTCTAGTGTTTCG+TGG + chr8.2:40413518-40413537 MS.gene25411:CDS 30.0%
ATTATGAGAGCTTGAAGTAT+AGG - chr8.2:40413793-40413812 None:intergenic 30.0%
TCTCATAATTCAGTATGACA+AGG + chr8.2:40413804-40413823 MS.gene25411:CDS 30.0%
TTAACTAAAAAGTGAGTAGG+GGG + chr8.2:40412935-40412954 MS.gene25411:intron 30.0%
! TTTAACTTAAGCTTTAGCGT+GGG + chr8.2:40413840-40413859 MS.gene25411:intron 30.0%
!! AGTTCCAAAGTTTCTAAGAT+TGG - chr8.2:40413233-40413252 None:intergenic 30.0%
!! GAGTTGATAAGTACATTTGT+TGG + chr8.2:40412666-40412685 MS.gene25411:intron 30.0%
AAATCACCAATTACTACCCT+CGG - chr8.2:40412812-40412831 None:intergenic 35.0%
AACAGATTCACTTTCAGAAG+CGG - chr8.2:40413462-40413481 None:intergenic 35.0%
AAGAGATTACATCTAGACAC+TGG + chr8.2:40413164-40413183 MS.gene25411:CDS 35.0%
ACGTACCAAAAGAGATTACT+TGG + chr8.2:40413649-40413668 MS.gene25411:intron 35.0%
CATCGTAGAAATCAAAAGCT+TGG - chr8.2:40412136-40412155 None:intergenic 35.0%
CTGTCCAATCTTAGAAACTT+TGG + chr8.2:40413226-40413245 MS.gene25411:CDS 35.0%
TGCAGGTTATATCACCATTT+CGG + chr8.2:40413907-40413926 MS.gene25411:intron 35.0%
TGCATTGCCAAATGTGATAT+TGG - chr8.2:40413387-40413406 None:intergenic 35.0%
TGTAATGTACACAGTCTCTA+CGG + chr8.2:40413089-40413108 MS.gene25411:intron 35.0%
TTGGCAATGCAACTTCTATT+GGG + chr8.2:40413396-40413415 MS.gene25411:CDS 35.0%
TTTGGCAATGCAACTTCTAT+TGG + chr8.2:40413395-40413414 MS.gene25411:CDS 35.0%
! GACTACCAAGTAATCTCTTT+TGG - chr8.2:40413657-40413676 None:intergenic 35.0%
! GTTTAACTTAAGCTTTAGCG+TGG + chr8.2:40413839-40413858 MS.gene25411:intron 35.0%
! TTCCTTTTCAACCGAATCTT+TGG + chr8.2:40413253-40413272 MS.gene25411:CDS 35.0%
!! CAAAGTTTCTAAGATTGGAC+AGG - chr8.2:40413228-40413247 None:intergenic 35.0%
!! CGAAGTTATCAAGTTGAGAT+TGG - chr8.2:40412277-40412296 None:intergenic 35.0%
!! GGCAAAGTCTTTGAAATGTT+CGG - chr8.2:40412186-40412205 None:intergenic 35.0%
ACAGAAAAGGGTCGAAAGTA+GGG - chr8.2:40413750-40413769 None:intergenic 40.0%
ACCATTTCGGTATAGAACAG+AGG + chr8.2:40413920-40413939 MS.gene25411:CDS 40.0%
ACGTACACAACAACAACAAG+TGG - chr8.2:40413633-40413652 None:intergenic 40.0%
AGCCTCACCAATATCACATT+TGG + chr8.2:40413377-40413396 MS.gene25411:CDS 40.0%
ATGTGATATTGGTGAGGCTA+AGG - chr8.2:40413376-40413395 None:intergenic 40.0%
GAAATGGTGATATAACCTGC+AGG - chr8.2:40413908-40413927 None:intergenic 40.0%
GAAATTTGACTCAGTTGGCA+AGG + chr8.2:40412590-40412609 MS.gene25411:intron 40.0%
GAAGAACGTCGTACAGAAAA+GGG - chr8.2:40413762-40413781 None:intergenic 40.0%
GATATAACCTGCAGGAAACA+AGG - chr8.2:40413900-40413919 None:intergenic 40.0%
GGCAGAGAGAAGGATATTAA+TGG - chr8.2:40413207-40413226 None:intergenic 40.0%
GTTTCGTGGTTATACAGCAA+AGG + chr8.2:40413532-40413551 MS.gene25411:CDS 40.0%
TACAGAAAAGGGTCGAAAGT+AGG - chr8.2:40413751-40413770 None:intergenic 40.0%
TATTGGGAACTTGCACAATG+TGG + chr8.2:40413412-40413431 MS.gene25411:CDS 40.0%
TCCTCTGTTCTATACCGAAA+TGG - chr8.2:40413924-40413943 None:intergenic 40.0%
TGAAGAACGTCGTACAGAAA+AGG - chr8.2:40413763-40413782 None:intergenic 40.0%
TGCCAAATGTGATATTGGTG+AGG - chr8.2:40413382-40413401 None:intergenic 40.0%
TGTCTTGAGATAAACACACC+TGG + chr8.2:40413341-40413360 MS.gene25411:CDS 40.0%
TGTGGAATTGGCACAATCTT+CGG + chr8.2:40413121-40413140 MS.gene25411:CDS 40.0%
! ACATCTAGACACTGGTAATG+TGG + chr8.2:40413172-40413191 MS.gene25411:CDS 40.0%
! GCTCTAGATGTAGTAAACAG+CGG - chr8.2:40413720-40413739 None:intergenic 40.0%
! TTTTAATGCCAGAGAGAGCT+TGG - chr8.2:40413505-40413524 None:intergenic 40.0%
ACGGTTACCTGATGAAGAAG+AGG - chr8.2:40412427-40412446 None:intergenic 45.0%
AGATGAAGATCCTCAAGGTG+GGG - chr8.2:40412341-40412360 None:intergenic 45.0%
ATCTCACTGAGTGACTCAGT+TGG + chr8.2:40412639-40412658 MS.gene25411:intron 45.0%
CCTAAGCTTGGCCAAAGATT+CGG - chr8.2:40413267-40413286 None:intergenic 45.0%
CGAATCTTTGGCCAAGCTTA+GGG + chr8.2:40413265-40413284 MS.gene25411:CDS 45.0%
CGGTGAGATCATTGTGGAAT+TGG + chr8.2:40413109-40413128 MS.gene25411:CDS 45.0%
CTCACAGGTATCTTCATCTC+TGG + chr8.2:40412102-40412121 MS.gene25411:CDS 45.0%
CTTGGAGGAGAGTGAGTAAA+GGG - chr8.2:40413487-40413506 None:intergenic 45.0%
GAGATGAAGATCCTCAAGGT+GGG - chr8.2:40412342-40412361 None:intergenic 45.0%
GCACTAACCTCTTCTTCATC+AGG + chr8.2:40412417-40412436 MS.gene25411:CDS 45.0%
TAGGATCCGAGGGTAGTAAT+TGG + chr8.2:40412803-40412822 MS.gene25411:intron 45.0%
TCTCTACGGTGAGATCATTG+TGG + chr8.2:40413103-40413122 MS.gene25411:CDS 45.0%
! ACTGAGTGACTCAGTTGGTA+AGG + chr8.2:40412644-40412663 MS.gene25411:intron 45.0%
! AGTTGTACCTTGTTTCCTGC+AGG + chr8.2:40413890-40413909 MS.gene25411:intron 45.0%
! CAAGGAAATCTAGGATCCGA+GGG + chr8.2:40412793-40412812 MS.gene25411:intron 45.0%
! GGCCAAAGATTCGGTTGAAA+AGG - chr8.2:40413258-40413277 None:intergenic 45.0%
! TCAAGGAAATCTAGGATCCG+AGG + chr8.2:40412792-40412811 MS.gene25411:intron 45.0%
!! TTAGTTAATAATAAATTATA+CGG - chr8.2:40412923-40412942 None:intergenic 5.0%
AGATTGGACAGGCAGAGAGA+AGG - chr8.2:40413217-40413236 None:intergenic 50.0%
CCGAATCTTTGGCCAAGCTT+AGG + chr8.2:40413264-40413283 MS.gene25411:CDS 50.0%
CGAGATGAAGATCCTCAAGG+TGG - chr8.2:40412343-40412362 None:intergenic 50.0%
GAATACTACCCCGATGTCGA+CGG + chr8.2:40412155-40412174 MS.gene25411:CDS 50.0%
GAGCGAGATGAAGATCCTCA+AGG - chr8.2:40412346-40412365 None:intergenic 50.0%
GCTTGGAGGAGAGTGAGTAA+AGG - chr8.2:40413488-40413507 None:intergenic 50.0%
GGAAGATGGAACCCTAAGCT+TGG - chr8.2:40413279-40413298 None:intergenic 50.0%
TAATGCCAGAGAGAGCTTGG+AGG - chr8.2:40413502-40413521 None:intergenic 50.0%
TGGGAACTTGCACAATGTGG+AGG + chr8.2:40413415-40413434 MS.gene25411:CDS 50.0%
! AGGCTAAGGTACTTGAGACC+AGG - chr8.2:40413362-40413381 None:intergenic 50.0%
! GAGTTTGAAGCCCTGATCCT+CGG - chr8.2:40412378-40412397 None:intergenic 50.0%
!! GAGGACGGCATTGACGAATA+TGG - chr8.2:40412207-40412226 None:intergenic 50.0%
ACGCATCTTCCGAATGTCGC+GGG - chr8.2:40412243-40412262 None:intergenic 55.0%
ACTCTCCTCCAAGCTCTCTC+TGG + chr8.2:40413494-40413513 MS.gene25411:CDS 55.0%
ATGCCGTCCTCTCTCTACGA+AGG + chr8.2:40412216-40412235 MS.gene25411:CDS 55.0%
CGGAAGATGCGTCTCTCTTG+CGG + chr8.2:40412251-40412270 MS.gene25411:CDS 55.0%
CTCTTCTCCTCCGAGGATCA+GGG + chr8.2:40412365-40412384 MS.gene25411:CDS 55.0%
CTGAACGCTCTGTTGACACG+TGG + chr8.2:40412297-40412316 MS.gene25411:CDS 55.0%
GACGCATCTTCCGAATGTCG+CGG - chr8.2:40412244-40412263 None:intergenic 55.0%
GGTCCTTCGTAGAGAGAGGA+CGG - chr8.2:40412222-40412241 None:intergenic 55.0%
TACGAAGGACCCGCGACATT+CGG + chr8.2:40412231-40412250 MS.gene25411:CDS 55.0%
TCCAAAGCTCACGAGCGACA+AGG - chr8.2:40412710-40412729 None:intergenic 55.0%
TCTCTTCTCCTCCGAGGATC+AGG + chr8.2:40412364-40412383 MS.gene25411:CDS 55.0%
TGCATGATAGCACCGCGAGT+GGG - chr8.2:40412400-40412419 None:intergenic 55.0%
! TTTGAAGCCCTGATCCTCGG+AGG - chr8.2:40412375-40412394 None:intergenic 55.0%
AAGCTCACGAGCGACAAGGC+AGG - chr8.2:40412706-40412725 None:intergenic 60.0%
GCCTTGTCGCTCGTGAGCTT+TGG + chr8.2:40412706-40412725 MS.gene25411:intron 60.0%
GGGCTTCAAACTCCCACTCG+CGG + chr8.2:40412385-40412404 MS.gene25411:CDS 60.0%
GTGCATGATAGCACCGCGAG+TGG - chr8.2:40412401-40412420 None:intergenic 60.0%
TCTCGCTCTCTTCTCCTCCG+AGG + chr8.2:40412358-40412377 MS.gene25411:CDS 60.0%
ACGTGGATCCGCACCGCTGT+TGG + chr8.2:40412314-40412333 MS.gene25411:CDS 65.0%
CGCGGGTCCTTCGTAGAGAG+AGG - chr8.2:40412226-40412245 None:intergenic 65.0%
CGTGGATCCGCACCGCTGTT+GGG + chr8.2:40412315-40412334 MS.gene25411:CDS 65.0%
CTCAAGGTGGGGCCCAACAG+CGG - chr8.2:40412330-40412349 None:intergenic 65.0%
TTCGGCCTCGCCGTCGACAT+CGG - chr8.2:40412168-40412187 None:intergenic 65.0%
CGCTGTTGGGCCCCACCTTG+AGG + chr8.2:40412328-40412347 MS.gene25411:CDS 70.0%
CTACCCCGATGTCGACGGCG+AGG + chr8.2:40412160-40412179 MS.gene25411:CDS 70.0%
TCGGCCTCGCCGTCGACATC+GGG - chr8.2:40412167-40412186 None:intergenic 70.0%
CGGCCTCGCCGTCGACATCG+GGG - chr8.2:40412166-40412185 None:intergenic 75.0%
GGTGGGGCCCAACAGCGGTG+CGG - chr8.2:40412325-40412344 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 40412089 40413953 40412089 ID=MS.gene25411
chr8.2 mRNA 40412089 40413953 40412089 ID=MS.gene25411.t1;Parent=MS.gene25411
chr8.2 exon 40412089 40412438 40412089 ID=MS.gene25411.t1.exon1;Parent=MS.gene25411.t1
chr8.2 CDS 40412089 40412438 40412089 ID=cds.MS.gene25411.t1;Parent=MS.gene25411.t1
chr8.2 exon 40413103 40413553 40413103 ID=MS.gene25411.t1.exon2;Parent=MS.gene25411.t1
chr8.2 CDS 40413103 40413553 40413103 ID=cds.MS.gene25411.t1;Parent=MS.gene25411.t1
chr8.2 exon 40413661 40413825 40413661 ID=MS.gene25411.t1.exon3;Parent=MS.gene25411.t1
chr8.2 CDS 40413661 40413825 40413661 ID=cds.MS.gene25411.t1;Parent=MS.gene25411.t1
chr8.2 exon 40413912 40413953 40413912 ID=MS.gene25411.t1.exon4;Parent=MS.gene25411.t1
chr8.2 CDS 40413912 40413953 40413912 ID=cds.MS.gene25411.t1;Parent=MS.gene25411.t1
Gene Sequence

>MS.gene25411

ATGAGTCTTGTTTCTCACAGGTATCTTCATCTCTGGAAACATCTCCAAGCTTTTGATTTCTACGATGAATACTACCCCGATGTCGACGGCGAGGCCGAACATTTCAAAGACTTTGCCATATTCGTCAATGCCGTCCTCTCTCTACGAAGGACCCGCGACATTCGGAAGATGCGTCTCTCTTGCGGCCAATCTCAACTTGATAACTTCGCTGAACGCTCTGTTGACACGTGGATCCGCACCGCTGTTGGGCCCCACCTTGAGGATCTTCATCTCGCTCTCTTCTCCTCCGAGGATCAGGGCTTCAAACTCCCACTCGCGGTGCTATCATGCACTAACCTCTTCTTCATCAGGTAACCGTTTTTTTTCCTTCATATTTGAGTAATAACAGTATTTTACTCTCATTTTCAATGTTTATTTGTTACAGAAAAATAGGTATAATGGGATAACAGATGATTAAAATTATTTTATGAAAAAGTAACAAAATTTGTTGATTTTAACAATGAAATTTGACTCAGTTGGCAAGGAGTTGATATGTAAGTACATTTGTTGGATCTCACTGAGTGACTCAGTTGGTAAGGAGTTGATAAGTACATTTGTTGGGTCTCATTGAGAGACCTGCCTTGTCGCTCGTGAGCTTTGGAGCCCAACTCACCTAAACTTTTTTAAAAAGCATTTATTGATTGTTTTGTTATATGAAAATAACTCAAGGAAATCTAGGATCCGAGGGTAGTAATTGGTGATTTTATGATCATGCTTGTGAAGCAAAGACATTTTCAATTAACAAATCAATTAATAAAAGATATGAATTTACAAAAATTACAGTCTTTTAGTCCGTATAATTTATTATTAACTAAAAAGTGAGTAGGGGGCATAGAAATATAATTAGTAAACAAGAAGGTAATTTAGAGCTACAAAGATATTTCAGTCATTTTTTTACTTATTATTTGCAAACTTGTTCTTGTTAAATGTGACATGATAAATGATGATAACATAATGTATGTGTAATGTACACAGTCTCTACGGTGAGATCATTGTGGAATTGGCACAATCTTCGGCTGTTTGTTTACCATCATTGAAGAGATTACATCTAGACACTGGTAATGTGGATGTGAATGCCATTAATATCCTTCTCTCTGCCTGTCCAATCTTAGAAACTTTGGAACTTTCCTTTTCAACCGAATCTTTGGCCAAGCTTAGGGTTCCATCTTCCTTGAAAAGTTTTAAATTTACTATAGAGAATGACATTGAAGTTTGTCTTGAGATAAACACACCTGGTCTCAAGTACCTTAGCCTCACCAATATCACATTTGGCAATGCAACTTCTATTGGGAACTTGCACAATGTGGAGGAAGCATATCTTGATGTACTCTCCGCTTCTGAAAGTGAATCTGTTGACCCTTTACTCACTCTCCTCCAAGCTCTCTCTGGCATTAAAAGTCTAGTGTTTCGTGGTTATACAGCAAAGGTGAGTGATATTTTCATTATCCTTGTGTATTATATATTCCATTATATGTAATAGTTTTGTGCTAATTCTTGTATCTCCACTTGTTGTTGTTGTGTACGTACCAAAAGAGATTACTTGGTAGTCAACCTTCTATAGATTTTTCGAAAATGCACTTTTCGGAATTCCGCTGTTTACTACATCTAGAGCTCATTGTCCCTACTTTCGACCCTTTTCTGTACGACGTTCTTCAGAAATGTCCTATACTTCAAGCTCTCATAATTCAGTATGACAAGGTTCGTAAATAGAGTTTAACTTAAGCTTTAGCGTGGGTGTGTGTTTTTGTTTGATTATTTGGTGAGTTGTACCTTGTTTCCTGCAGGTTATATCACCATTTCGGTATAGAACAGAGGACAGTGATTGA
Protein sequence

>MS.gene25411.t1

MSLVSHRYLHLWKHLQAFDFYDEYYPDVDGEAEHFKDFAIFVNAVLSLRRTRDIRKMRLSCGQSQLDNFAERSVDTWIRTAVGPHLEDLHLALFSSEDQGFKLPLAVLSCTNLFFISLYGEIIVELAQSSAVCLPSLKRLHLDTGNVDVNAINILLSACPILETLELSFSTESLAKLRVPSSLKSFKFTIENDIEVCLEINTPGLKYLSLTNITFGNATSIGNLHNVEEAYLDVLSASESESVDPLLTLLQALSGIKSLVFRGYTAKRLLGSQPSIDFSKMHFSEFRCLLHLELIVPTFDPFLYDVLQKCPILQALIIQYDKVISPFRYRTEDSD