AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72956

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72956.t1 MTR_8g468810 78.554 415 86 2 1 414 51 463 0.0 650
MS.gene72956.t1 MTR_8g058970 62.587 433 143 3 1 414 45 477 0.0 517
MS.gene72956.t1 MTR_8g468810 81.115 323 58 2 1 322 51 371 0.0 517
MS.gene72956.t1 MTR_8g468810 81.749 263 46 1 1 263 51 311 1.02e-149 427
MS.gene72956.t1 MTR_8g468810 81.749 263 46 1 1 263 51 311 1.62e-149 427
MS.gene72956.t1 MTR_8g468810 81.749 263 46 1 1 263 51 311 2.77e-149 427
MS.gene72956.t1 MTR_2g083840 54.067 418 181 6 1 414 47 457 1.40e-140 409
MS.gene72956.t1 MTR_8g062270 47.897 428 193 12 1 413 41 453 2.69e-114 342
MS.gene72956.t1 MTR_4g032730 46.136 440 192 9 3 413 43 466 1.78e-112 338
MS.gene72956.t1 MTR_8g468840 47.381 420 201 9 2 412 40 448 2.97e-108 326
MS.gene72956.t1 MTR_5g092960 49.302 430 184 13 3 413 47 461 8.56e-108 325
MS.gene72956.t1 MTR_8g468760 48.478 427 164 13 1 413 34 418 5.11e-106 320
MS.gene72956.t1 MTR_8g467800 47.541 427 191 12 2 414 46 453 8.12e-105 318
MS.gene72956.t1 MTR_8g014260 43.262 423 213 15 4 414 44 451 1.89e-95 293
MS.gene72956.t1 MTR_8g445780 44.414 367 180 9 4 364 41 389 2.94e-81 255
MS.gene72956.t1 MTR_5g092960 50.000 334 144 10 3 322 47 371 7.56e-78 246
MS.gene72956.t1 MTR_8g469020 38.106 433 233 12 2 414 34 451 3.50e-77 246
MS.gene72956.t1 MTR_8g058965 38.785 428 236 10 2 414 40 456 2.10e-75 242
MS.gene72956.t1 MTR_8g070950 37.875 433 211 15 1 414 41 434 4.85e-71 230
MS.gene72956.t1 MTR_4g050250 40.838 382 190 10 1 356 47 418 2.17e-69 225
MS.gene72956.t1 MTR_8g467800 51.111 270 120 6 2 266 46 308 7.06e-66 214
MS.gene72956.t1 MTR_8g062010 36.058 416 244 11 1 414 35 430 8.72e-63 208
MS.gene72956.t1 MTR_8g062080 36.321 424 233 13 1 414 49 445 1.69e-62 208
MS.gene72956.t1 MTR_4g024710 42.193 301 160 8 117 414 58 347 1.89e-58 194
MS.gene72956.t1 MTR_8g014160 33.412 422 209 11 2 414 67 425 5.73e-58 196
MS.gene72956.t1 MTR_8g062310 35.266 414 226 13 4 414 54 428 6.85e-58 196
MS.gene72956.t1 MTR_8g062300 34.744 449 235 16 1 414 52 477 3.26e-54 187
MS.gene72956.t1 MTR_4g024745 41.259 286 154 8 115 397 10 284 3.15e-48 166
MS.gene72956.t1 MTR_8g014210 43.568 241 126 6 174 414 1 231 2.19e-46 159
MS.gene72956.t1 MTR_8g467790 34.146 369 177 13 1 354 174 491 6.34e-44 160
MS.gene72956.t1 MTR_8g077460 41.451 193 107 4 4 191 38 229 9.57e-36 135
MS.gene72956.t1 MTR_8g077460 33.209 268 150 9 155 414 129 375 2.89e-29 117
MS.gene72956.t1 MTR_5g021550 50.000 116 55 3 4 116 47 162 6.28e-28 108
MS.gene72956.t1 MTR_5g080710 28.066 424 264 15 2 413 59 453 9.83e-25 105
MS.gene72956.t1 MTR_8g014290 43.089 123 61 4 4 117 38 160 3.34e-23 95.5
MS.gene72956.t1 MTR_4g056040 29.630 405 237 16 13 388 65 450 6.82e-23 100
MS.gene72956.t1 MTR_2g086020 27.885 416 197 13 1 414 1 315 1.04e-22 98.2
MS.gene72956.t1 MTR_4g113810 36.471 170 101 5 57 221 1 168 1.63e-21 91.3
MS.gene72956.t1 MTR_5g084070 27.532 385 237 14 2 376 39 391 9.60e-21 94.0
MS.gene72956.t1 MTR_4g055990 28.610 374 224 14 41 389 106 461 1.13e-20 94.4
MS.gene72956.t1 MTR_4g055990 28.610 374 224 14 41 389 106 461 1.37e-20 94.0
MS.gene72956.t1 MTR_4g056070 30.484 351 194 16 41 364 109 436 2.94e-20 92.8
MS.gene72956.t1 MTR_8g014220 42.975 121 57 5 46 161 47 160 2.03e-17 79.7
MS.gene72956.t1 MTR_4g056030 28.493 365 211 15 41 378 69 410 3.18e-17 83.6
MS.gene72956.t1 MTR_4g056020 28.371 356 225 14 41 379 69 411 2.75e-16 80.9
MS.gene72956.t1 MTR_4g055580 27.671 365 208 14 44 378 199 537 2.92e-15 78.2
MS.gene72956.t1 MTR_1g038710 26.705 352 227 11 48 378 85 426 4.76e-15 77.0
MS.gene72956.t1 MTR_4g024720 35.075 134 64 6 4 116 45 176 2.26e-14 72.0
MS.gene72956.t1 MTR_1g041245 31.361 169 99 4 2 166 37 192 4.25e-13 70.9
MS.gene72956.t1 MTR_1g041245 32.609 138 80 4 286 414 253 386 9.00e-11 63.5
MS.gene72956.t1 MTR_8g014230 48.000 75 35 2 330 404 25 95 3.09e-12 63.2
MS.gene72956.t1 MTR_7g115270 22.353 425 252 14 2 411 38 399 9.31e-12 66.6
MS.gene72956.t1 MTR_3g108560 25.178 421 220 17 2 409 46 384 7.14e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72956.t1 AT4G26340 28.103 427 258 16 2 414 28 419 7.30e-26 108
MS.gene72956.t1 AT1G78750 26.682 431 276 15 2 411 44 455 6.77e-23 100
MS.gene72956.t1 AT1G78750 26.682 431 276 15 2 411 44 455 6.77e-23 100
MS.gene72956.t1 AT1G66300 28.409 440 242 21 3 411 56 453 7.58e-20 91.7
MS.gene72956.t1 AT4G13985 26.106 452 254 19 1 411 44 456 1.77e-19 90.5
MS.gene72956.t1 AT5G38590 27.179 390 232 14 2 376 28 380 2.12e-19 90.1
MS.gene72956.t1 AT1G51370 27.529 425 240 23 12 411 51 432 8.79e-19 88.2
MS.gene72956.t1 AT1G66290 27.064 436 252 21 2 411 55 450 1.12e-18 88.2
MS.gene72956.t1 AT5G56420 25.000 436 259 18 2 414 32 422 1.82e-18 87.4
MS.gene72956.t1 AT5G56420 25.000 436 259 18 2 414 32 422 1.82e-18 87.4
MS.gene72956.t1 AT5G56420 25.000 436 259 18 2 414 32 422 1.82e-18 87.4
MS.gene72956.t1 AT5G56370 26.267 434 257 18 2 413 28 420 2.16e-18 87.0
MS.gene72956.t1 AT5G56370 26.267 434 257 18 2 413 28 420 2.16e-18 87.0
MS.gene72956.t1 AT5G22730 27.815 453 241 22 2 411 54 463 4.70e-18 86.3
MS.gene72956.t1 AT5G22730 27.815 453 241 22 2 411 54 463 4.70e-18 86.3
MS.gene72956.t1 AT3G50710 25.635 433 267 17 3 414 29 427 1.04e-17 85.1
MS.gene72956.t1 AT5G38570 26.128 421 227 16 3 413 29 375 1.50e-17 84.3
MS.gene72956.t1 AT4G10400 25.882 425 258 20 2 413 28 408 1.77e-17 84.3
MS.gene72956.t1 AT4G10400 25.882 425 258 20 2 413 28 408 1.77e-17 84.3
MS.gene72956.t1 AT4G10400 25.882 425 258 20 2 413 28 408 1.77e-17 84.3
MS.gene72956.t1 AT1G32375 25.926 432 262 17 3 414 29 422 3.49e-17 83.6
MS.gene72956.t1 AT1G32375 25.926 432 262 17 3 414 29 422 3.49e-17 83.6
MS.gene72956.t1 AT3G54910 26.582 395 246 13 31 414 12 373 5.63e-17 82.4
MS.gene72956.t1 AT3G54910 26.582 395 246 13 31 414 12 373 5.63e-17 82.4
MS.gene72956.t1 AT3G54910 26.582 395 246 13 31 414 12 373 5.63e-17 82.4
MS.gene72956.t1 AT5G60610 25.935 401 234 15 2 380 28 387 4.20e-16 80.1
MS.gene72956.t1 AT5G60610 25.935 401 234 15 2 380 28 387 4.20e-16 80.1
MS.gene72956.t1 AT1G06630 29.137 278 170 10 2 266 38 301 7.32e-16 78.2
MS.gene72956.t1 AT5G38590 29.707 239 147 9 2 233 28 252 8.84e-16 77.8
MS.gene72956.t1 AT1G06630 27.928 333 207 12 2 319 38 352 9.70e-16 78.6
MS.gene72956.t1 AT1G06630 27.928 333 207 12 2 319 38 352 9.70e-16 78.6
MS.gene72956.t1 AT1G06630 27.844 334 208 12 2 320 47 362 1.18e-15 78.6
MS.gene72956.t1 AT1G06630 27.844 334 208 12 2 320 38 353 1.24e-15 78.6
MS.gene72956.t1 AT1G06630 27.844 334 208 12 2 320 38 353 1.24e-15 78.6
MS.gene72956.t1 AT1G06630 27.844 334 208 12 2 320 38 353 1.24e-15 78.6
MS.gene72956.t1 AT1G06630 27.844 334 208 12 2 320 38 353 1.24e-15 78.6
MS.gene72956.t1 AT1G06630 27.844 334 208 12 2 320 38 353 1.57e-15 78.6
MS.gene72956.t1 AT5G22720 27.174 460 243 22 2 413 49 464 1.63e-15 78.6
MS.gene72956.t1 AT5G22670 30.189 371 214 19 30 376 58 407 2.44e-15 77.8
MS.gene72956.t1 AT1G55660 24.130 431 286 17 2 411 79 489 5.28e-15 77.0
MS.gene72956.t1 AT3G58920 23.729 472 270 18 2 414 28 468 9.27e-15 76.3
MS.gene72956.t1 AT4G10420 25.723 346 222 13 77 413 2 321 1.61e-14 74.3
MS.gene72956.t1 AT5G56700 27.005 374 232 16 14 375 2 346 2.66e-14 74.3
MS.gene72956.t1 AT5G56810 27.146 431 253 18 2 411 41 431 5.72e-14 73.6
MS.gene72956.t1 AT3G52680 25.172 437 267 18 2 414 47 447 6.17e-14 73.6
MS.gene72956.t1 AT5G44950 27.696 408 235 20 2 380 30 406 8.75e-14 73.2
MS.gene72956.t1 AT2G26030 26.829 451 252 23 2 411 30 443 1.65e-13 72.4
MS.gene72956.t1 AT2G26860 25.783 415 240 18 2 385 28 405 1.96e-13 72.0
MS.gene72956.t1 AT2G26860 25.783 415 240 18 2 385 28 405 1.96e-13 72.0
MS.gene72956.t1 AT5G38590 30.808 198 125 6 41 233 27 217 2.36e-13 70.1
MS.gene72956.t1 AT4G00315 24.884 430 284 17 2 411 28 438 5.79e-13 70.5
MS.gene72956.t1 AT1G55660 27.982 218 141 7 2 212 79 287 6.43e-13 70.1
MS.gene72956.t1 AT5G56390 24.476 429 272 14 3 413 30 424 1.40e-12 69.3
MS.gene72956.t1 AT1G22000 25.197 381 222 15 2 356 55 398 2.12e-12 68.9
MS.gene72956.t1 AT1G13780 26.619 417 239 20 2 380 36 423 3.19e-12 68.6
MS.gene72956.t1 AT3G49480 26.115 314 194 12 80 375 114 407 3.85e-12 68.2
MS.gene72956.t1 AT3G26920 27.954 347 216 15 78 413 16 339 6.28e-12 67.0
MS.gene72956.t1 AT3G26920 27.954 347 216 15 78 413 16 339 6.28e-12 67.0
MS.gene72956.t1 AT3G54160 25.359 418 254 17 24 412 36 424 7.77e-12 67.0
MS.gene72956.t1 AT5G50270 24.877 406 255 17 3 389 30 404 1.05e-11 66.6
MS.gene72956.t1 AT3G49480 26.829 287 176 11 103 375 1 267 1.31e-11 65.5
MS.gene72956.t1 AT4G14103 29.675 246 145 10 2 234 34 264 2.04e-11 65.5
MS.gene72956.t1 AT4G14103 29.675 246 145 10 2 234 34 264 2.04e-11 65.5
MS.gene72956.t1 AT4G09920 29.091 275 161 16 2 264 28 280 2.30e-11 65.1
MS.gene72956.t1 AT4G09920 29.091 275 161 16 2 264 28 280 2.30e-11 65.1
MS.gene72956.t1 AT5G56400 24.541 436 262 19 4 414 62 455 2.35e-11 65.9
MS.gene72956.t1 AT3G49030 25.058 431 241 17 2 413 47 414 2.63e-11 65.5
MS.gene72956.t1 AT4G14096 27.324 355 218 14 12 344 40 376 2.77e-11 65.5
MS.gene72956.t1 AT4G14096 27.324 355 218 14 12 344 40 376 2.77e-11 65.5
MS.gene72956.t1 AT4G14103 29.675 246 145 10 2 234 34 264 3.80e-11 65.1
MS.gene72956.t1 AT5G27750 24.070 457 273 18 2 414 31 457 3.88e-11 65.1
MS.gene72956.t1 AT4G09920 28.832 274 167 15 2 262 28 286 5.75e-11 63.5
MS.gene72956.t1 AT4G09920 28.832 274 167 15 2 262 28 286 5.75e-11 63.5
MS.gene72956.t1 AT4G09920 28.832 274 167 15 2 262 28 286 5.75e-11 63.5
MS.gene72956.t1 AT5G56370 26.642 274 170 10 2 269 28 276 5.93e-11 63.9
MS.gene72956.t1 AT4G00160 25.694 432 263 17 2 410 42 438 6.70e-11 64.3

Find 89 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGACCTATATCCACTTTAA+AGG 0.185801 8.3:+41883509 MS.gene72956:CDS
CGGTGAGATCTTTGTAGAAT+TGG 0.284338 8.3:+41882640 MS.gene72956:CDS
ACGTACCAAAAGAGATTATT+AGG 0.292927 8.3:+41883176 MS.gene72956:intron
CCTCTTCTCGTCTGAGGATC+AGG 0.298567 8.3:+41881797 MS.gene72956:CDS
CTTGATGTAGTCTCCTCTTC+TGG 0.309388 8.3:+41882977 MS.gene72956:CDS
CACCACATCAATTTGGTCTT+AGG 0.331226 8.3:+41883447 MS.gene72956:CDS
AAGTGGGATGCAAGACAATT+AGG 0.332413 8.3:-41883488 None:intergenic
CTGAATCTTTGGCCAAGCTT+AGG 0.379532 8.3:+41882795 MS.gene72956:CDS
GGCAGAGAGAAGGATATTAA+TGG 0.390004 8.3:-41882735 None:intergenic
TACGAAGGACCCGCGACATT+CGG 0.394870 8.3:+41881664 MS.gene72956:CDS
AGCAAAGTCTTTGAAATGTT+CGG 0.401003 8.3:-41881616 None:intergenic
AGTAAATTCAAAACTCTTCA+AGG 0.409402 8.3:-41882828 None:intergenic
GGTGAGATCTTTGTAGAATT+GGG 0.418728 8.3:+41882641 MS.gene72956:CDS
ACTCTCCTCCAAGCGCTCTC+TGG 0.418872 8.3:+41883025 MS.gene72956:CDS
TTCGGCCTCGCCGTCGACGC+CGG 0.419368 8.3:-41881598 None:intergenic
TGAATCTTTGGCCAAGCTTA+GGG 0.420723 8.3:+41882796 MS.gene72956:CDS
CGTGGATTCGCACCGCCGTT+GGG 0.424276 8.3:+41881748 MS.gene72956:CDS
GATACAGCACCACATCAATT+TGG 0.425006 8.3:+41883440 MS.gene72956:CDS
AGCCTCACTGATATCACTAT+TGG 0.428235 8.3:+41882908 MS.gene72956:CDS
TCGGCCTCGCCGTCGACGCC+GGG 0.429312 8.3:-41881597 None:intergenic
CTCATAGGTATCTTCATCTC+TGG 0.437215 8.3:+41881535 MS.gene72956:CDS
GGCCAAAGATTCAGTTGAAA+AGG 0.438596 8.3:-41882786 None:intergenic
TGAAGTTATCAAGTTGAGAT+TGG 0.445049 8.3:-41881707 None:intergenic
AGAGGAGGTTAGTGCATGAT+AGG 0.446173 8.3:-41881842 None:intergenic
ATTATAAGAGCTTCAAGTAT+AGG 0.447586 8.3:-41883317 None:intergenic
AGTTTCAAAGTTTCTAAGAC+TGG 0.448465 8.3:-41882761 None:intergenic
ATTTAACTCCAGAGAGCGCT+TGG 0.458373 8.3:-41883033 None:intergenic
TTGGCGATGCCACTTCTGTT+GGG 0.460446 8.3:+41882927 MS.gene72956:CDS
ATCCTCTATATCCTTTAAAG+TGG 0.461419 8.3:-41883520 None:intergenic
ATCCACTTTAAAGGATATAG+AGG 0.462211 8.3:+41883518 MS.gene72956:CDS
TCAAAACTCTTCAAGGAAGA+TGG 0.465651 8.3:-41882821 None:intergenic
GCACTAACCTCCTCTCCATC+AGG 0.467643 8.3:+41881850 MS.gene72956:CDS
CAATGAGCCTTGTCTCTCAT+AGG 0.474674 8.3:+41881520 None:intergenic
CTTGGAGGAGAGTGAGTAAA+GGG 0.475202 8.3:-41883015 None:intergenic
ACGTGGATTCGCACCGCCGT+TGG 0.482549 8.3:+41881747 MS.gene72956:CDS
TTCTACAATGAGTACTCTCC+CGG 0.484115 8.3:+41881579 MS.gene72956:CDS
ATTGGCGATGCCACTTCTGT+TGG 0.491458 8.3:+41882926 MS.gene72956:CDS
TGTAGAATTGGGACAATCAT+CGG 0.493103 8.3:+41882652 MS.gene72956:CDS
ATATCCTTTAAAGTGGATAT+AGG 0.498926 8.3:-41883513 None:intergenic
CATTGTAGAAATGAAAAGCT+TGG 0.503238 8.3:-41881566 None:intergenic
CAAAGTTTCTAAGACTGGAC+AGG 0.505834 8.3:-41882756 None:intergenic
TTAAAGGATATAGAGGATGT+TGG 0.507552 8.3:+41883525 MS.gene72956:CDS
TAGAGGATGTTGGCTTGAGA+TGG 0.521759 8.3:+41883535 MS.gene72956:CDS
AGTTGTAATCTCTTCAATGA+TGG 0.525654 8.3:-41882686 None:intergenic
TAGTGATATCAGTGAGGCTA+AGG 0.528202 8.3:-41882904 None:intergenic
TACAGAAAAGCGTCGAAAGT+AGG 0.534200 8.3:-41883275 None:intergenic
CTCTTCTCGTCTGAGGATCA+GGG 0.539018 8.3:+41881798 MS.gene72956:CDS
ATCCTCAAGGTACGGCCCAA+CGG 0.545220 8.3:-41881763 None:intergenic
CGCCGTTGGGCCGTACCTTG+AGG 0.554002 8.3:+41881761 MS.gene72956:CDS
CCTGATCCTCAGACGAGAAG+AGG 0.554039 8.3:-41881797 None:intergenic
ACGCATCTTCCGAATGTCGC+GGG 0.555891 8.3:-41881673 None:intergenic
TGTAATGCACACAGTCTCTA+CGG 0.564381 8.3:+41882620 MS.gene72956:intron
TCTCGACCTCTTCTCGTCTG+AGG 0.568024 8.3:+41881791 MS.gene72956:CDS
AACAGATTCACTTCCAGAAG+AGG 0.569559 8.3:-41882990 None:intergenic
TAACTCCAGAGAGCGCTTGG+AGG 0.571531 8.3:-41883030 None:intergenic
GCTTGGAGGAGAGTGAGTAA+AGG 0.575040 8.3:-41883016 None:intergenic
TAAAGTGGATATAGGTCAAG+TGG 0.575798 8.3:-41883505 None:intergenic
GGAAGATGGAACCCTAAGCT+TGG 0.579963 8.3:-41882807 None:intergenic
GTGCAAGTTCCCAACAGAAG+TGG 0.580230 8.3:-41882936 None:intergenic
AGACTGGACAGGCAGAGAGA+AGG 0.581233 8.3:-41882745 None:intergenic
CTGAACGCTCTGTTGACACG+TGG 0.585988 8.3:+41881730 MS.gene72956:CDS
GAGTACTCTCCCGGCGTCGA+CGG 0.586422 8.3:+41881588 MS.gene72956:CDS
AGATGAAGATCCTCAAGGTA+CGG 0.599815 8.3:-41881771 None:intergenic
AAGAGATTACAACTAGACAT+TGG 0.609472 8.3:+41882695 MS.gene72956:CDS
CGGAAGATGCGTCTCTCTTG+CGG 0.610368 8.3:+41881684 MS.gene72956:CDS
GAACTTAGTCATATTTAACT+CGG 0.618369 8.3:-41883700 None:intergenic
GGGCTTCAAACTCCCTCTCG+CGG 0.620327 8.3:+41881818 MS.gene72956:CDS
ACAACTAGACATTGGTAATG+TGG 0.620834 8.3:+41882703 MS.gene72956:CDS
AGGCTAAGGTACTTGAGACC+AGG 0.622331 8.3:-41882890 None:intergenic
CTCTCCCGGCGTCGACGGCG+AGG 0.630012 8.3:+41881593 MS.gene72956:CDS
CTCAAGGTACGGCCCAACGG+CGG 0.630217 8.3:-41881760 None:intergenic
GACGCATCTTCCGAATGTCG+CGG 0.638940 8.3:-41881674 None:intergenic
AAAGTGGATATAGGTCAAGT+GGG 0.643217 8.3:-41883504 None:intergenic
ACGGCTTTCTCTCTTTACGA+AGG 0.645259 8.3:+41881649 MS.gene72956:CDS
GTTTAGTTGTAATACAACAA+TGG 0.648312 8.3:+41883063 MS.gene72956:CDS
TCTTATAATTCAGAATGACA+AGG 0.648619 8.3:+41883331 MS.gene72956:CDS
AAAACGGTAACCTGATGGAG+AGG 0.651756 8.3:-41881860 None:intergenic
ACGGTAACCTGATGGAGAGG+AGG 0.652142 8.3:-41881857 None:intergenic
TGTTGGGAACTTGCACAACG+TGG 0.655593 8.3:+41882943 MS.gene72956:CDS
TGCATGATAGGACCGCGAGA+GGG 0.662917 8.3:-41881830 None:intergenic
GTCCTAAGACCAAATTGATG+TGG 0.669559 8.3:-41883449 None:intergenic
GTGCATGATAGGACCGCGAG+AGG 0.674731 8.3:-41881831 None:intergenic
TGTCTTGAGATAGACACACC+TGG 0.677521 8.3:+41882872 MS.gene72956:CDS
GAAGATACCTATGAGAGACA+AGG 0.684686 8.3:-41881527 None:intergenic
GGTCGAGATGAAGATCCTCA+AGG 0.701781 8.3:-41881776 None:intergenic
TGGGAACTTGCACAACGTGG+AGG 0.706891 8.3:+41882946 MS.gene72956:CDS
CGCCAATAGTGATATCAGTG+AGG 0.714173 8.3:-41882910 None:intergenic
CGGCAGACAGTAGATCCCCG+TGG 0.745934 8.3:-41883680 None:intergenic
AAAATTTCGTATATGCCACG+GGG 0.751789 8.3:+41883665 MS.gene72956:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTTTTTTTTTTTTGAA+AGG - chr8.3:41882032-41882051 None:intergenic 10.0%
!! TATAAAGGAATATATAATCA+TGG - chr8.3:41883108-41883127 None:intergenic 15.0%
!!! ATGTTTTTTTTAGAAAGTTT+AGG - chr8.3:41882219-41882238 None:intergenic 15.0%
!!! TTGTCATATCTTTTATTAAT+TGG - chr8.3:41882358-41882377 None:intergenic 15.0%
!! AATATGAAGGGAAAAAAAAA+CGG - chr8.3:41881879-41881898 None:intergenic 20.0%
!! ACTAAAATACTCAAATATGA+AGG - chr8.3:41881892-41881911 None:intergenic 20.0%
!! CTAAAATACTCAAATATGAA+GGG - chr8.3:41881891-41881910 None:intergenic 20.0%
!! GAAGACTACTAAATATATAA+AGG - chr8.3:41883123-41883142 None:intergenic 20.0%
!!! ACGAAATTTTTTTGAGAAAA+TGG - chr8.3:41883655-41883674 None:intergenic 20.0%
!!! TTTTTATTCTACTATTGTAC+TGG + chr8.3:41882467-41882486 MS.gene72956:intron 20.0%
! AAAAAATTTCGTATATGCCA+CGG + chr8.3:41883663-41883682 MS.gene72956:CDS 25.0%
! AGTAAATTCAAAACTCTTCA+AGG - chr8.3:41882831-41882850 None:intergenic 25.0%
! ATTATAAGAGCTTCAAGTAT+AGG - chr8.3:41883320-41883339 None:intergenic 25.0%
! GTTTAGTTGTAATACAACAA+TGG + chr8.3:41883063-41883082 MS.gene72956:CDS 25.0%
! TCTTATAATTCAGAATGACA+AGG + chr8.3:41883331-41883350 MS.gene72956:CDS 25.0%
! TGTTATATGAAAATAACTCG+AGG + chr8.3:41882251-41882270 MS.gene72956:intron 25.0%
!!! ATATCCTTTAAAGTGGATAT+AGG - chr8.3:41883516-41883535 None:intergenic 25.0%
!!! GTGTTGTTTGTTTGATTATT+TGG + chr8.3:41883393-41883412 MS.gene72956:intron 25.0%
!!! TTTTTCGAAAATGCACTTTT+CGG + chr8.3:41883217-41883236 MS.gene72956:CDS 25.0%
AAAAAAAAACGGTAACCTGA+TGG - chr8.3:41881868-41881887 None:intergenic 30.0%
AAAAATTTCGTATATGCCAC+GGG + chr8.3:41883664-41883683 MS.gene72956:CDS 30.0%
AAAATAACTCGAGGAAAACT+AGG + chr8.3:41882260-41882279 MS.gene72956:intron 30.0%
AAGAGATTACAACTAGACAT+TGG + chr8.3:41882695-41882714 MS.gene72956:CDS 30.0%
ACGTACCAAAAGAGATTATT+AGG + chr8.3:41883176-41883195 MS.gene72956:intron 30.0%
AGTTGTAATCTCTTCAATGA+TGG - chr8.3:41882689-41882708 None:intergenic 30.0%
CATTGTAGAAATGAAAAGCT+TGG - chr8.3:41881569-41881588 None:intergenic 30.0%
TACAGAGAGTTAGGTATAAT+GGG + chr8.3:41881936-41881955 MS.gene72956:intron 30.0%
TTAAAGGATATAGAGGATGT+TGG + chr8.3:41883525-41883544 MS.gene72956:CDS 30.0%
TTAGAAAGTTTAGGTGAGTT+GGG - chr8.3:41882210-41882229 None:intergenic 30.0%
TTGACCTATATCCACTTTAA+AGG + chr8.3:41883509-41883528 MS.gene72956:CDS 30.0%
TTTAGAAAGTTTAGGTGAGT+TGG - chr8.3:41882211-41882230 None:intergenic 30.0%
! AGACAATTAGGAACACTTTT+CGG - chr8.3:41883479-41883498 None:intergenic 30.0%
! ATCCACTTTAAAGGATATAG+AGG + chr8.3:41883518-41883537 MS.gene72956:CDS 30.0%
! ATCCTCTATATCCTTTAAAG+TGG - chr8.3:41883523-41883542 None:intergenic 30.0%
! GACTACCTAATAATCTCTTT+TGG - chr8.3:41883184-41883203 None:intergenic 30.0%
! GCTAGTTATGTTTTGCATTA+TGG + chr8.3:41883563-41883582 MS.gene72956:CDS 30.0%
! TTCCTTTTCAACTGAATCTT+TGG + chr8.3:41882784-41882803 MS.gene72956:CDS 30.0%
! TTGAAGAAAATGCGTTTTTC+TGG + chr8.3:41883593-41883612 MS.gene72956:CDS 30.0%
! TTTATTTGCTACAGAGAGTT+AGG + chr8.3:41881927-41881946 MS.gene72956:intron 30.0%
!! AGATATGACAAAACTTGCTA+AGG + chr8.3:41882367-41882386 MS.gene72956:intron 30.0%
!! AGCAAAGTCTTTGAAATGTT+CGG - chr8.3:41881619-41881638 None:intergenic 30.0%
!! AGTTTCAAAGTTTCTAAGAC+TGG - chr8.3:41882764-41882783 None:intergenic 30.0%
!! ATTGAGTTTAACTTGAGCTT+TGG + chr8.3:41883361-41883380 MS.gene72956:intron 30.0%
!! TGAAGTTATCAAGTTGAGAT+TGG - chr8.3:41881710-41881729 None:intergenic 30.0%
AAAATTTCGTATATGCCACG+GGG + chr8.3:41883665-41883684 MS.gene72956:CDS 35.0%
AAAGTGGATATAGGTCAAGT+GGG - chr8.3:41883507-41883526 None:intergenic 35.0%
AGAGATTCCTGCATCAATAA+TGG - chr8.3:41882113-41882132 None:intergenic 35.0%
ATCACCAATTACTACTCCAT+CGG - chr8.3:41882288-41882307 None:intergenic 35.0%
CTACAGAGAGTTAGGTATAA+TGG + chr8.3:41881935-41881954 MS.gene72956:intron 35.0%
GAGATTCCTGCATCAATAAT+GGG - chr8.3:41882112-41882131 None:intergenic 35.0%
GATCTGTAAGTACGTTTGTT+GGG + chr8.3:41882142-41882161 MS.gene72956:intron 35.0%
GGTGAGATCTTTGTAGAATT+GGG + chr8.3:41882641-41882660 MS.gene72956:CDS 35.0%
TAAAGTGGATATAGGTCAAG+TGG - chr8.3:41883508-41883527 None:intergenic 35.0%
TATTGATGCAGGAATCTCTT+TGG + chr8.3:41882114-41882133 MS.gene72956:intron 35.0%
TCAAAACTCTTCAAGGAAGA+TGG - chr8.3:41882824-41882843 None:intergenic 35.0%
TGATCTGTAAGTACGTTTGT+TGG + chr8.3:41882141-41882160 MS.gene72956:intron 35.0%
TGTAGAATTGGGACAATCAT+CGG + chr8.3:41882652-41882671 MS.gene72956:CDS 35.0%
TTAGACATTCACTTGCAGAA+TGG + chr8.3:41882443-41882462 MS.gene72956:intron 35.0%
!! ACAACTAGACATTGGTAATG+TGG + chr8.3:41882703-41882722 MS.gene72956:CDS 35.0%
!! TTTAACTTGAGCTTTGGTGT+GGG + chr8.3:41883367-41883386 MS.gene72956:intron 35.0%
!!! GACTTTGCTATTTTCGTCAA+CGG + chr8.3:41881630-41881649 MS.gene72956:CDS 35.0%
!!! GCGTTTTTCTGGTTTTTTGT+TGG + chr8.3:41883604-41883623 MS.gene72956:CDS 35.0%
AACAGATTCACTTCCAGAAG+AGG - chr8.3:41882993-41883012 None:intergenic 40.0%
AAGTGGGATGCAAGACAATT+AGG - chr8.3:41883491-41883510 None:intergenic 40.0%
ACAATGAAAGTTGACTGCGT+TGG + chr8.3:41882050-41882069 MS.gene72956:intron 40.0%
AGATGAAGATCCTCAAGGTA+CGG - chr8.3:41881774-41881793 None:intergenic 40.0%
AGCCTCACTGATATCACTAT+TGG + chr8.3:41882908-41882927 MS.gene72956:CDS 40.0%
CGGTGAGATCTTTGTAGAAT+TGG + chr8.3:41882640-41882659 MS.gene72956:CDS 40.0%
CTCATAGGTATCTTCATCTC+TGG + chr8.3:41881535-41881554 MS.gene72956:CDS 40.0%
GAAGATACCTATGAGAGACA+AGG - chr8.3:41881530-41881549 None:intergenic 40.0%
GATACAGCACCACATCAATT+TGG + chr8.3:41883440-41883459 MS.gene72956:CDS 40.0%
GGCAGAGAGAAGGATATTAA+TGG - chr8.3:41882738-41882757 None:intergenic 40.0%
GGCCAAAGATTCAGTTGAAA+AGG - chr8.3:41882789-41882808 None:intergenic 40.0%
GGTACGTACAAAACAACAAG+TGG - chr8.3:41883163-41883182 None:intergenic 40.0%
GTCCTAAGACCAAATTGATG+TGG - chr8.3:41883452-41883471 None:intergenic 40.0%
TAGTGATATCAGTGAGGCTA+AGG - chr8.3:41882907-41882926 None:intergenic 40.0%
TCAACTCCCATTATTGATGC+AGG + chr8.3:41882103-41882122 MS.gene72956:intron 40.0%
TGAATCTTTGGCCAAGCTTA+GGG + chr8.3:41882796-41882815 MS.gene72956:CDS 40.0%
TGTAATGCACACAGTCTCTA+CGG + chr8.3:41882620-41882639 MS.gene72956:intron 40.0%
TTCTACAATGAGTACTCTCC+CGG + chr8.3:41881579-41881598 MS.gene72956:CDS 40.0%
! CACCACATCAATTTGGTCTT+AGG + chr8.3:41883447-41883466 MS.gene72956:CDS 40.0%
!! CAAAGTTTCTAAGACTGGAC+AGG - chr8.3:41882759-41882778 None:intergenic 40.0%
!! GTTTAACTTGAGCTTTGGTG+TGG + chr8.3:41883366-41883385 MS.gene72956:intron 40.0%
!! TACAGAAAAGCGTCGAAAGT+AGG - chr8.3:41883278-41883297 None:intergenic 40.0%
AAAACGGTAACCTGATGGAG+AGG - chr8.3:41881863-41881882 None:intergenic 45.0%
ACGGCTTTCTCTCTTTACGA+AGG + chr8.3:41881649-41881668 MS.gene72956:CDS 45.0%
AGAGGAGGTTAGTGCATGAT+AGG - chr8.3:41881845-41881864 None:intergenic 45.0%
CGCCAATAGTGATATCAGTG+AGG - chr8.3:41882913-41882932 None:intergenic 45.0%
CTGAATCTTTGGCCAAGCTT+AGG + chr8.3:41882795-41882814 MS.gene72956:CDS 45.0%
CTTGATGTAGTCTCCTCTTC+TGG + chr8.3:41882977-41882996 MS.gene72956:CDS 45.0%
CTTGGAGGAGAGTGAGTAAA+GGG - chr8.3:41883018-41883037 None:intergenic 45.0%
GGATCCGATGGAGTAGTAAT+TGG + chr8.3:41882281-41882300 MS.gene72956:intron 45.0%
GTAGAGTTTACTCACCAGCA+AGG + chr8.3:41882072-41882091 MS.gene72956:intron 45.0%
TGTCTTGAGATAGACACACC+TGG + chr8.3:41882872-41882891 MS.gene72956:CDS 45.0%
! AGTTGTACCTTGTTTCCTGC+AGG + chr8.3:41883418-41883437 MS.gene72956:intron 45.0%
!! ATTTAACTCCAGAGAGCGCT+TGG - chr8.3:41883036-41883055 None:intergenic 45.0%
!! TAGAGGATGTTGGCTTGAGA+TGG + chr8.3:41883535-41883554 MS.gene72956:CDS 45.0%
AGTTGATCTCACCACCTTGC+TGG - chr8.3:41882089-41882108 None:intergenic 50.0%
CGAGGAAAACTAGGATCCGA+TGG + chr8.3:41882269-41882288 MS.gene72956:intron 50.0%
CTCTTCTCGTCTGAGGATCA+GGG + chr8.3:41881798-41881817 MS.gene72956:CDS 50.0%
GAGTTTACTCACCAGCAAGG+TGG + chr8.3:41882075-41882094 MS.gene72956:intron 50.0%
GCTGTATCCTGCAGGAAACA+AGG - chr8.3:41883428-41883447 None:intergenic 50.0%
GCTTGGAGGAGAGTGAGTAA+AGG - chr8.3:41883019-41883038 None:intergenic 50.0%
GGAAGATGGAACCCTAAGCT+TGG - chr8.3:41882810-41882829 None:intergenic 50.0%
GGTCGAGATGAAGATCCTCA+AGG - chr8.3:41881779-41881798 None:intergenic 50.0%
GTGCAAGTTCCCAACAGAAG+TGG - chr8.3:41882939-41882958 None:intergenic 50.0%
TGTTGGGAACTTGCACAACG+TGG + chr8.3:41882943-41882962 MS.gene72956:CDS 50.0%
! AGGCTAAGGTACTTGAGACC+AGG - chr8.3:41882893-41882912 None:intergenic 50.0%
! ATTGGCGATGCCACTTCTGT+TGG + chr8.3:41882926-41882945 MS.gene72956:CDS 50.0%
! TTGGCGATGCCACTTCTGTT+GGG + chr8.3:41882927-41882946 MS.gene72956:CDS 50.0%
ACGCATCTTCCGAATGTCGC+GGG - chr8.3:41881676-41881695 None:intergenic 55.0%
ACGGTAACCTGATGGAGAGG+AGG - chr8.3:41881860-41881879 None:intergenic 55.0%
AGACTGGACAGGCAGAGAGA+AGG - chr8.3:41882748-41882767 None:intergenic 55.0%
ATCCTCAAGGTACGGCCCAA+CGG - chr8.3:41881766-41881785 None:intergenic 55.0%
CCTCTTCTCGTCTGAGGATC+AGG + chr8.3:41881797-41881816 MS.gene72956:CDS 55.0%
CCTGATCCTCAGACGAGAAG+AGG - chr8.3:41881800-41881819 None:intergenic 55.0%
CGGAAGATGCGTCTCTCTTG+CGG + chr8.3:41881684-41881703 MS.gene72956:CDS 55.0%
CTGAACGCTCTGTTGACACG+TGG + chr8.3:41881730-41881749 MS.gene72956:CDS 55.0%
GACGCATCTTCCGAATGTCG+CGG - chr8.3:41881677-41881696 None:intergenic 55.0%
GCACTAACCTCCTCTCCATC+AGG + chr8.3:41881850-41881869 MS.gene72956:CDS 55.0%
TACGAAGGACCCGCGACATT+CGG + chr8.3:41881664-41881683 MS.gene72956:CDS 55.0%
TCTCGACCTCTTCTCGTCTG+AGG + chr8.3:41881791-41881810 MS.gene72956:CDS 55.0%
TGCATGATAGGACCGCGAGA+GGG - chr8.3:41881833-41881852 None:intergenic 55.0%
TGGGAACTTGCACAACGTGG+AGG + chr8.3:41882946-41882965 MS.gene72956:CDS 55.0%
!! GATGTGGTGCTGTATCCTGC+AGG - chr8.3:41883436-41883455 None:intergenic 55.0%
!! TAACTCCAGAGAGCGCTTGG+AGG - chr8.3:41883033-41883052 None:intergenic 55.0%
ACTCTCCTCCAAGCGCTCTC+TGG + chr8.3:41883025-41883044 MS.gene72956:CDS 60.0%
AGAGACCTGCCTTGTCGCTC+GGG + chr8.3:41882173-41882192 MS.gene72956:intron 60.0%
GAGAGACCTGCCTTGTCGCT+CGG + chr8.3:41882172-41882191 MS.gene72956:intron 60.0%
GGGCTTCAAACTCCCTCTCG+CGG + chr8.3:41881818-41881837 MS.gene72956:CDS 60.0%
GTGCATGATAGGACCGCGAG+AGG - chr8.3:41881834-41881853 None:intergenic 60.0%
AAGCTCCCGAGCGACAAGGC+AGG - chr8.3:41882181-41882200 None:intergenic 65.0%
ACGTGGATTCGCACCGCCGT+TGG + chr8.3:41881747-41881766 MS.gene72956:CDS 65.0%
CCAGAAGCTCCCGAGCGACA+AGG - chr8.3:41882185-41882204 None:intergenic 65.0%
CCTTGTCGCTCGGGAGCTTC+TGG + chr8.3:41882182-41882201 MS.gene72956:intron 65.0%
CGGCAGACAGTAGATCCCCG+TGG - chr8.3:41883683-41883702 None:intergenic 65.0%
CGTGGATTCGCACCGCCGTT+GGG + chr8.3:41881748-41881767 MS.gene72956:CDS 65.0%
CTCAAGGTACGGCCCAACGG+CGG - chr8.3:41881763-41881782 None:intergenic 65.0%
! GAGTACTCTCCCGGCGTCGA+CGG + chr8.3:41881588-41881607 MS.gene72956:CDS 65.0%
CGCCGTTGGGCCGTACCTTG+AGG + chr8.3:41881761-41881780 MS.gene72956:CDS 70.0%
TTCGGCCTCGCCGTCGACGC+CGG - chr8.3:41881601-41881620 None:intergenic 75.0%
! TCGGCCTCGCCGTCGACGCC+GGG - chr8.3:41881600-41881619 None:intergenic 80.0%
!!! CTCTCCCGGCGTCGACGGCG+AGG + chr8.3:41881593-41881612 MS.gene72956:CDS 80.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 41881522 41883718 41881522 ID=MS.gene72956
chr8.3 mRNA 41881522 41883718 41881522 ID=MS.gene72956.t1;Parent=MS.gene72956
chr8.3 exon 41881522 41881871 41881522 ID=MS.gene72956.t1.exon1;Parent=MS.gene72956.t1
chr8.3 CDS 41881522 41881871 41881522 ID=cds.MS.gene72956.t1;Parent=MS.gene72956.t1
chr8.3 exon 41882634 41883084 41882634 ID=MS.gene72956.t1.exon2;Parent=MS.gene72956.t1
chr8.3 CDS 41882634 41883084 41882634 ID=cds.MS.gene72956.t1;Parent=MS.gene72956.t1
chr8.3 exon 41883188 41883352 41883188 ID=MS.gene72956.t1.exon3;Parent=MS.gene72956.t1
chr8.3 CDS 41883188 41883352 41883188 ID=cds.MS.gene72956.t1;Parent=MS.gene72956.t1
chr8.3 exon 41883440 41883718 41883440 ID=MS.gene72956.t1.exon4;Parent=MS.gene72956.t1
chr8.3 CDS 41883440 41883718 41883440 ID=cds.MS.gene72956.t1;Parent=MS.gene72956.t1
Gene Sequence

>MS.gene72956

ATGAGCCTTGTCTCTCATAGGTATCTTCATCTCTGGAAATATCTCCAAGCTTTTCATTTCTACAATGAGTACTCTCCCGGCGTCGACGGCGAGGCCGAACATTTCAAAGACTTTGCTATTTTCGTCAACGGCTTTCTCTCTTTACGAAGGACCCGCGACATTCGGAAGATGCGTCTCTCTTGCGGCCAATCTCAACTTGATAACTTCACTGAACGCTCTGTTGACACGTGGATTCGCACCGCCGTTGGGCCGTACCTTGAGGATCTTCATCTCGACCTCTTCTCGTCTGAGGATCAGGGCTTCAAACTCCCTCTCGCGGTCCTATCATGCACTAACCTCCTCTCCATCAGGTTACCGTTTTTTTTTCCCTTCATATTTGAGTATTTTAGTCTCTCATTTTCAATGTTTATTTGCTACAGAGAGTTAGGTATAATGGGATAACAAATTATTAAAATTATTTTATGAAAAAGTAACAAGATTTATTGATTTTAACAATGAAAGTTGACTCCTTTCAAAAAAAAAAAAAAAACAATGAAAGTTGACTGCGTTGGTAGAGTTTACTCACCAGCAAGGTGGTGAGATCAACTCCCATTATTGATGCAGGAATCTCTTTGGTAGTTGATCTGTAAGTACGTTTGTTGGGTCTCACTGAGAGACCTGCCTTGTCGCTCGGGAGCTTCTGGAGCCCAACTCACCTAAACTTTCTAAAAAAAACATTTATTGATTGTTTGTTATATGAAAATAACTCGAGGAAAACTAGGATCCGATGGAGTAGTAATTGGTGATTTTATGATTATTATGCTAATTAAGCAAAGACATTTTCAATTAACAAACCAATTAATAAAAGATATGACAAAACTTGCTAAGGAAAAGACACTATGTCATGTTTTGTAGCATTGTTACATACATTCAACATTATTCTTAGACATTCACTTGCAGAATGGCTTTTTATTCTACTATTGTACTGGTATTTATTTACAAAAATTACAGTCTTTTTTAGAGCTTCAAAGATATTTCTTTCAGTCATTTCTTTATTTATTATTTGCACACTTGTTCTTGTTAAATGTGACATGATAAATGATGATAACATAAGATTTGTGTAATGCACACAGTCTCTACGGTGAGATCTTTGTAGAATTGGGACAATCATCGGCTGTTTGTTTACCATCATTGAAGAGATTACAACTAGACATTGGTAATGTGGATGTGAATGCCATTAATATCCTTCTCTCTGCCTGTCCAGTCTTAGAAACTTTGAAACTTTCCTTTTCAACTGAATCTTTGGCCAAGCTTAGGGTTCCATCTTCCTTGAAGAGTTTTGAATTTACTGTAGAGAATGAAATTGAAGTTTGTCTTGAGATAGACACACCTGGTCTCAAGTACCTTAGCCTCACTGATATCACTATTGGCGATGCCACTTCTGTTGGGAACTTGCACAACGTGGAGGAAGCATATCTTGATGTAGTCTCCTCTTCTGGAAGTGAATCTGTTGACCCTTTACTCACTCTCCTCCAAGCGCTCTCTGGAGTTAAATGTCTAGTGTTTAGTTGTAATACAACAATGGTGAGTGCAATTTTCGTTATCCATGATTATATATTCCTTTATATATTTAGTAGTCTTCTGCTAATTCTTGTATCTCCACTTGTTGTTTTGTACGTACCAAAAGAGATTATTAGGTAGTCAACGTATTATAGATTTTTCGAAAATGCACTTTTCGGAATTTCGCTGTTTACTACATCTAGAGCTCATTCTTCCTACTTTCGACGCTTTTCTGTACGTCGTGCTTCAGAAATGTCCTATACTTGAAGCTCTTATAATTCAGAATGACAAGGTTTGTGAATTGAGTTTAACTTGAGCTTTGGTGTGGGCGTGTGTTGTTTGTTTGATTATTTGGTGAGTTGTACCTTGTTTCCTGCAGGATACAGCACCACATCAATTTGGTCTTAGGACAAAGCCGAAAAGTGTTCCTAATTGTCTTGCATCCCACTTGACCTATATCCACTTTAAAGGATATAGAGGATGTTGGCTTGAGATGGACTTTGCTAGTTATGTTTTGCATTATGGACTTGTTTTGAAGAAAATGCGTTTTTCTGGTTTTTTGTTGGATGAAAGAAAGAAGTCGACAAAGTACCATTTTCTCAAAAAAATTTCGTATATGCCACGGGGATCTACTGTCTGCCGAGTTAAATATGACTAA
Protein sequence

>MS.gene72956.t1

MSLVSHRYLHLWKYLQAFHFYNEYSPGVDGEAEHFKDFAIFVNGFLSLRRTRDIRKMRLSCGQSQLDNFTERSVDTWIRTAVGPYLEDLHLDLFSSEDQGFKLPLAVLSCTNLLSISLYGEIFVELGQSSAVCLPSLKRLQLDIGNVDVNAINILLSACPVLETLKLSFSTESLAKLRVPSSLKSFEFTVENEIEVCLEIDTPGLKYLSLTDITIGDATSVGNLHNVEEAYLDVVSSSGSESVDPLLTLLQALSGVKCLVFSCNTTMRLLGSQRIIDFSKMHFSEFRCLLHLELILPTFDAFLYVVLQKCPILEALIIQNDKDTAPHQFGLRTKPKSVPNCLASHLTYIHFKGYRGCWLEMDFASYVLHYGLVLKKMRFSGFLLDERKKSTKYHFLKKISYMPRGSTVCRVKYD