AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072871


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072871.t1 MTR_3g106740 98.462 130 2 0 1 130 1 130 1.83e-85 245
MS.gene072871.t1 MTR_1g012690 59.091 154 37 3 1 129 1 153 9.54e-51 158
MS.gene072871.t1 MTR_1g012700 57.317 164 34 3 1 129 1 163 1.26e-50 158
MS.gene072871.t1 MTR_3g106750 73.282 131 30 2 1 129 1 128 1.63e-48 152
MS.gene072871.t1 MTR_1g012710 59.868 152 38 2 1 130 1 151 8.63e-48 150
MS.gene072871.t1 MTR_1g012630 78.571 84 17 1 46 129 127 209 4.82e-40 133
MS.gene072871.t1 MTR_4g108150 52.459 122 53 2 8 129 6 122 5.54e-35 117
MS.gene072871.t1 MTR_4g101260 51.969 127 55 3 8 129 6 131 7.44e-34 115
MS.gene072871.t1 MTR_2g026760 60.360 111 40 4 21 129 18 126 1.19e-33 114
MS.gene072871.t1 MTR_4g101280 63.218 87 30 2 44 129 57 142 2.53e-31 108
MS.gene072871.t1 MTR_4g101330 63.953 86 29 2 45 129 84 168 3.38e-30 107
MS.gene072871.t1 MTR_4g101310 60.000 85 32 2 46 129 50 133 2.55e-29 103
MS.gene072871.t1 MTR_4g101270 58.824 85 32 3 47 130 105 187 1.03e-23 90.9
MS.gene072871.t1 MTR_5g012340 44.828 87 45 2 44 129 92 176 8.45e-23 88.2
MS.gene072871.t1 MTR_5g012380 43.678 87 46 2 44 129 85 169 3.64e-22 86.7
MS.gene072871.t1 MTR_2g026920 45.679 81 41 2 50 130 118 195 2.45e-17 74.3
MS.gene072871.t1 MTR_2g026930 46.250 80 40 2 50 129 122 198 2.47e-17 74.7
MS.gene072871.t1 MTR_3g082100 44.828 87 47 1 44 130 251 336 1.60e-15 71.6
MS.gene072871.t1 MTR_2g026775 48.718 78 34 3 54 129 686 759 2.15e-15 71.6
MS.gene072871.t1 MTR_4g115360 54.023 87 37 3 44 129 148 232 5.27e-13 63.5
MS.gene072871.t1 MTR_2g026820 52.113 71 29 3 52 121 542 608 1.85e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072871.t1 AT2G45180 74.419 86 21 1 44 129 49 133 3.27e-37 123
MS.gene072871.t1 AT1G62510 68.235 85 25 2 46 129 66 149 7.74e-33 113
MS.gene072871.t1 AT4G12490 41.758 182 52 4 1 129 1 181 7.86e-32 111
MS.gene072871.t1 AT4G12480 64.368 87 29 2 44 129 82 167 1.01e-31 110
MS.gene072871.t1 AT4G12470 61.111 90 33 2 42 130 73 161 1.75e-31 110
MS.gene072871.t1 AT5G46890 54.962 131 52 5 1 129 1 126 1.89e-31 108
MS.gene072871.t1 AT4G12500 64.368 87 29 2 44 129 91 176 2.45e-31 110
MS.gene072871.t1 AT5G46900 69.663 89 23 3 41 129 42 126 2.66e-31 108
MS.gene072871.t1 AT1G12090 68.293 82 24 2 49 129 57 137 3.57e-31 108
MS.gene072871.t1 AT4G00165 54.808 104 43 3 29 129 26 128 5.86e-31 107
MS.gene072871.t1 AT4G00165 54.808 104 43 3 29 129 26 128 5.86e-31 107
MS.gene072871.t1 AT4G12520 54.198 131 55 5 1 129 1 128 2.93e-30 105
MS.gene072871.t1 AT4G12510 54.198 131 55 5 1 129 1 128 2.93e-30 105
MS.gene072871.t1 AT4G22460 51.852 108 52 0 23 130 26 133 3.48e-29 103
MS.gene072871.t1 AT4G12550 56.977 86 35 2 45 129 26 110 2.27e-28 100
MS.gene072871.t1 AT1G12100 43.651 126 65 4 7 129 9 131 3.60e-27 98.2
MS.gene072871.t1 AT4G12545 56.180 89 34 3 42 129 23 107 7.49e-26 94.0
MS.gene072871.t1 AT4G12530 47.126 87 45 1 44 130 31 116 9.77e-23 86.3
MS.gene072871.t1 AT3G22142 56.977 86 32 4 47 130 1397 1479 1.04e-22 92.8
MS.gene072871.t1 AT2G10940 55.172 87 36 3 44 129 205 289 1.32e-21 87.4
MS.gene072871.t1 AT2G10940 55.172 87 36 3 44 129 205 289 1.32e-21 87.4
MS.gene072871.t1 AT3G22120 49.412 85 41 2 47 130 267 350 1.48e-18 80.1
MS.gene072871.t1 AT4G15160 50.000 86 40 3 47 130 99 183 1.74e-18 77.4
MS.gene072871.t1 AT3G22120 49.412 85 41 2 47 130 250 333 2.54e-18 79.3
MS.gene072871.t1 AT4G15160 50.000 86 40 3 47 130 181 265 5.52e-18 77.8
MS.gene072871.t1 AT4G22490 37.500 96 53 3 37 130 27 117 6.63e-16 68.9
MS.gene072871.t1 AT1G62500 43.529 85 45 3 47 130 212 294 6.34e-15 69.7

Find 42 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAATGTTGTGCATTGATTA+AGG 0.202840 3.2:-87429870 MS.gene072871:CDS
AGCACAAACCCCTAGCTTTA+AGG 0.218235 3.2:+87429943 None:intergenic
GGGTTGGTTAATGTTGTTAT+TGG 0.240421 3.2:-87429911 MS.gene072871:CDS
TCCTTAGGGCATTGACCTTT+GGG 0.253417 3.2:+87429968 None:intergenic
ATCCTTAGGGCATTGACCTT+TGG 0.263717 3.2:+87429967 None:intergenic
GATTAAGGGTTTGGCTGATT+TGG 0.309202 3.2:-87429855 MS.gene072871:CDS
AAGAGAAAGCACTAAAATTA+AGG 0.315935 3.2:+87430072 None:intergenic
TTGTCTTTGCACTGCACTTA+AGG 0.338630 3.2:-87429822 MS.gene072871:CDS
CCTAGCTTTAAGGTATCCTT+AGG 0.341849 3.2:+87429953 None:intergenic
AAATGTTGTGCATTGATTAA+GGG 0.358491 3.2:-87429869 MS.gene072871:CDS
GAAGGTGTTGGCTTTGGTGA+TGG 0.414445 3.2:+87430013 None:intergenic
CCTTTGGGTGAAGGTGGAAC+AGG 0.427685 3.2:+87429983 None:intergenic
GTTATTGGAAACCCTCCTTC+AGG 0.428430 3.2:-87429896 MS.gene072871:CDS
TTGTGCATTGATTAAGGGTT+TGG 0.429971 3.2:-87429864 MS.gene072871:CDS
AGGAGCACGCTGAGCGTGAT+AGG 0.432511 3.2:+87429770 None:intergenic
GGGCATTGACCTTTGGGTGA+AGG 0.436950 3.2:+87429974 None:intergenic
AATTAAGGCAGAGAATTTGT+TGG 0.438270 3.2:+87430087 None:intergenic
GCAGGTGAAGGTGTTGGCTT+TGG 0.458002 3.2:+87430007 None:intergenic
TAAGGGTTTGGCTGATTTGG+AGG 0.464987 3.2:-87429852 MS.gene072871:CDS
ATTGGAATCCTTAGGGACAT+TGG 0.481064 3.2:+87429714 None:intergenic
GGTGAAGGTGGAACAGGAGC+AGG 0.481606 3.2:+87429989 None:intergenic
GCACTTAAGGCTAATGTGCT+TGG 0.487972 3.2:-87429809 MS.gene072871:CDS
CCTGGTTTCCAATGTCCCTA+AGG 0.488513 3.2:-87429722 None:intergenic
CCTAAGGATACCTTAAAGCT+AGG 0.505679 3.2:-87429953 MS.gene072871:CDS
CTAAGGATACCTTAAAGCTA+GGG 0.512185 3.2:-87429952 MS.gene072871:CDS
GGTGTTGGCTTTGGTGATGG+TGG 0.522065 3.2:+87430016 None:intergenic
CATTGACCTTTGGGTGAAGG+TGG 0.538821 3.2:+87429977 None:intergenic
CCTTAGGGACATTGGAAACC+AGG 0.543402 3.2:+87429722 None:intergenic
ACATTGGAAACCAGGAGGGA+CGG 0.567925 3.2:+87429730 None:intergenic
CCTGTTCCACCTTCACCCAA+AGG 0.572122 3.2:-87429983 MS.gene072871:CDS
CTAGCTTTAAGGTATCCTTA+GGG 0.574702 3.2:+87429954 None:intergenic
AACATTTACTACCTGAAGGA+GGG 0.575828 3.2:+87429885 None:intergenic
ACAGGAGCAGGTGAAGGTGT+TGG 0.576951 3.2:+87430001 None:intergenic
TGATGGTGGACAATTAGCTT+CGG 0.580942 3.2:+87430030 None:intergenic
ACCCAAAGGTCAATGCCCTA+AGG 0.586732 3.2:-87429969 MS.gene072871:CDS
GGTGGAACAGGAGCAGGTGA+AGG 0.595926 3.2:+87429995 None:intergenic
AGCTTCGGTGAATGTTGAGT+AGG 0.595958 3.2:+87430045 None:intergenic
CAACATTTACTACCTGAAGG+AGG 0.605436 3.2:+87429884 None:intergenic
GCACAACATTTACTACCTGA+AGG 0.630292 3.2:+87429881 None:intergenic
TAAGGATACCTTAAAGCTAG+GGG 0.646982 3.2:-87429951 MS.gene072871:CDS
AGGGACATTGGAAACCAGGA+GGG 0.669232 3.2:+87429726 None:intergenic
TAGGGACATTGGAAACCAGG+AGG 0.715268 3.2:+87429725 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATGTTGTGCATTGATTAA+GGG - chr3.2:87429949-87429968 MS.gene072871:CDS 25.0%
!!! AAGAGAAAGCACTAAAATTA+AGG + chr3.2:87429749-87429768 None:intergenic 25.0%
!!! TAAATGTTGTGCATTGATTA+AGG - chr3.2:87429948-87429967 MS.gene072871:CDS 25.0%
AATTAAGGCAGAGAATTTGT+TGG + chr3.2:87429734-87429753 None:intergenic 30.0%
AACATTTACTACCTGAAGGA+GGG + chr3.2:87429936-87429955 None:intergenic 35.0%
CTAAGGATACCTTAAAGCTA+GGG - chr3.2:87429866-87429885 MS.gene072871:CDS 35.0%
TAAGGATACCTTAAAGCTAG+GGG - chr3.2:87429867-87429886 MS.gene072871:CDS 35.0%
!! CTAGCTTTAAGGTATCCTTA+GGG + chr3.2:87429867-87429886 None:intergenic 35.0%
!! GGGTTGGTTAATGTTGTTAT+TGG - chr3.2:87429907-87429926 MS.gene072871:CDS 35.0%
!! TTGTGCATTGATTAAGGGTT+TGG - chr3.2:87429954-87429973 MS.gene072871:CDS 35.0%
CAACATTTACTACCTGAAGG+AGG + chr3.2:87429937-87429956 None:intergenic 40.0%
CCTAAGGATACCTTAAAGCT+AGG - chr3.2:87429865-87429884 MS.gene072871:CDS 40.0%
GATTAAGGGTTTGGCTGATT+TGG - chr3.2:87429963-87429982 MS.gene072871:CDS 40.0%
GCACAACATTTACTACCTGA+AGG + chr3.2:87429940-87429959 None:intergenic 40.0%
TTGTCTTTGCACTGCACTTA+AGG - chr3.2:87429996-87430015 MS.gene072871:CDS 40.0%
!! CCTAGCTTTAAGGTATCCTT+AGG + chr3.2:87429868-87429887 None:intergenic 40.0%
!! GGGTTTGTGCTGATCTTTTA+GGG - chr3.2:87429887-87429906 MS.gene072871:CDS 40.0%
!! TGATGGTGGACAATTAGCTT+CGG + chr3.2:87429791-87429810 None:intergenic 40.0%
!!! CGGTTTTTTGACATGCACTA+AGG + chr3.2:87430071-87430090 None:intergenic 40.0%
!!! TTGTGCTGATCTTTTAGGGT+TGG - chr3.2:87429891-87429910 MS.gene072871:CDS 40.0%
AGCTTCGGTGAATGTTGAGT+AGG + chr3.2:87429776-87429795 None:intergenic 45.0%
GCACTTAAGGCTAATGTGCT+TGG - chr3.2:87430009-87430028 MS.gene072871:CDS 45.0%
GTTATTGGAAACCCTCCTTC+AGG - chr3.2:87429922-87429941 MS.gene072871:CDS 45.0%
!! AGCACAAACCCCTAGCTTTA+AGG + chr3.2:87429878-87429897 None:intergenic 45.0%
!! ATCCTTAGGGCATTGACCTT+TGG + chr3.2:87429854-87429873 None:intergenic 45.0%
!! GGGGTTTGTGCTGATCTTTT+AGG - chr3.2:87429886-87429905 MS.gene072871:CDS 45.0%
!! TAAGGGTTTGGCTGATTTGG+AGG - chr3.2:87429966-87429985 MS.gene072871:CDS 45.0%
!! TCCTTAGGGCATTGACCTTT+GGG + chr3.2:87429853-87429872 None:intergenic 45.0%
ACATTGGAAACCAGGAGGGA+CGG + chr3.2:87430091-87430110 None:intergenic 50.0%
ACCCAAAGGTCAATGCCCTA+AGG - chr3.2:87429849-87429868 MS.gene072871:CDS 50.0%
AGGGACATTGGAAACCAGGA+GGG + chr3.2:87430095-87430114 None:intergenic 50.0%
CATTGACCTTTGGGTGAAGG+TGG + chr3.2:87429844-87429863 None:intergenic 50.0%
TAGGGACATTGGAAACCAGG+AGG + chr3.2:87430096-87430115 None:intergenic 50.0%
TGTCAAAAAACCGTCCCTCC+TGG - chr3.2:87430078-87430097 MS.gene072871:CDS 50.0%
!! GAAGGTGTTGGCTTTGGTGA+TGG + chr3.2:87429808-87429827 None:intergenic 50.0%
CCTGTTCCACCTTCACCCAA+AGG - chr3.2:87429835-87429854 MS.gene072871:CDS 55.0%
CCTTTGGGTGAAGGTGGAAC+AGG + chr3.2:87429838-87429857 None:intergenic 55.0%
! ACAGGAGCAGGTGAAGGTGT+TGG + chr3.2:87429820-87429839 None:intergenic 55.0%
!! GCAGGTGAAGGTGTTGGCTT+TGG + chr3.2:87429814-87429833 None:intergenic 55.0%
!! GGGCATTGACCTTTGGGTGA+AGG + chr3.2:87429847-87429866 None:intergenic 55.0%
!! GGTGTTGGCTTTGGTGATGG+TGG + chr3.2:87429805-87429824 None:intergenic 55.0%
GGTGAAGGTGGAACAGGAGC+AGG + chr3.2:87429832-87429851 None:intergenic 60.0%
GGTGGAACAGGAGCAGGTGA+AGG + chr3.2:87429826-87429845 None:intergenic 60.0%
! AGGAGCACGCTGAGCGTGAT+AGG + chr3.2:87430051-87430070 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 87429724 87430116 87429724 ID=MS.gene072871
chr3.2 mRNA 87429724 87430116 87429724 ID=MS.gene072871.t1;Parent=MS.gene072871
chr3.2 exon 87429724 87430116 87429724 ID=MS.gene072871.t1.exon1;Parent=MS.gene072871.t1
chr3.2 CDS 87429724 87430116 87429724 ID=cds.MS.gene072871.t1;Parent=MS.gene072871.t1
Gene Sequence

>MS.gene072871

ATGGCTTCCAACAAATTCTCTGCCTTAATTTTAGTGCTTTCTCTTCTTGCCTACTCAACATTCACCGAAGCTAATTGTCCACCATCACCAAAGCCAACACCTTCACCTGCTCCTGTTCCACCTTCACCCAAAGGTCAATGCCCTAAGGATACCTTAAAGCTAGGGGTTTGTGCTGATCTTTTAGGGTTGGTTAATGTTGTTATTGGAAACCCTCCTTCAGGTAGTAAATGTTGTGCATTGATTAAGGGTTTGGCTGATTTGGAGGCTGCACTTTGTCTTTGCACTGCACTTAAGGCTAATGTGCTTGGAATCAACTTGAACGTGCCTATCACGCTCAGCGTGCTCCTTAGTGCATGTCAAAAAACCGTCCCTCCTGGTTTCCAATGTCCCTAA

Protein sequence

>MS.gene072871.t1

MASNKFSALILVLSLLAYSTFTEANCPPSPKPTPSPAPVPPSPKGQCPKDTLKLGVCADLLGLVNVVIGNPPSGSKCCALIKGLADLEAALCLCTALKANVLGINLNVPITLSVLLSACQKTVPPGFQCP