AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene40534


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40534.t1 MTR_5g005200 88.811 143 6 1 1 133 1 143 4.24e-78 227
MS.gene40534.t1 MTR_5g025500 42.105 95 55 0 38 132 37 131 1.58e-24 91.7
MS.gene40534.t1 MTR_3g053350 51.327 113 11 5 21 129 29 101 1.16e-18 81.3
MS.gene40534.t1 MTR_3g055330 41.111 90 51 2 44 132 147 235 1.33e-15 71.6
MS.gene40534.t1 MTR_3g055330 41.111 90 51 2 44 132 147 235 2.04e-15 71.2
MS.gene40534.t1 MTR_5g018010 40.580 69 40 1 62 130 61 128 4.10e-15 69.7
MS.gene40534.t1 MTR_5g018010 37.805 82 50 1 51 132 183 263 2.13e-14 67.8
MS.gene40534.t1 MTR_5g018010 40.580 69 40 1 62 130 138 205 1.18e-14 69.3
MS.gene40534.t1 MTR_5g018010 37.805 82 50 1 51 132 260 340 5.25e-14 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene40534.t1 AT2G14880 55.944 143 50 6 1 132 1 141 4.37e-42 136
MS.gene40534.t1 AT4G34290 56.034 116 39 2 29 132 29 144 1.03e-34 117
MS.gene40534.t1 AT3G03590 52.564 78 37 0 55 132 63 140 2.18e-25 94.0
MS.gene40534.t1 AT1G31760 40.594 101 59 1 32 131 8 108 6.58e-25 92.0
MS.gene40534.t1 AT2G35605 47.253 91 48 0 41 131 15 105 1.22e-24 91.3
MS.gene40534.t1 AT2G14880 56.471 85 32 4 13 94 21 103 3.94e-21 82.0
MS.gene40534.t1 AT2G14880 56.471 85 32 4 13 94 21 103 3.94e-21 82.0
MS.gene40534.t1 AT4G26810 34.000 100 61 2 32 131 1 95 1.57e-15 67.8
MS.gene40534.t1 AT4G26810 34.000 100 61 2 32 131 1 95 1.57e-15 67.8
MS.gene40534.t1 AT4G22360 37.624 101 54 3 40 132 173 272 1.90e-15 71.6
MS.gene40534.t1 AT1G49520 37.209 86 50 2 45 130 243 324 1.87e-13 66.2
MS.gene40534.t1 AT1G49520 32.895 76 50 1 55 130 101 175 1.99e-11 60.5
MS.gene40534.t1 AT3G48600 33.019 106 67 2 32 133 8 113 6.20e-13 62.8
MS.gene40534.t1 AT3G19080 35.165 91 56 2 40 130 250 337 8.30e-13 64.3
MS.gene40534.t1 AT3G19080 34.211 76 49 1 55 130 115 189 1.26e-11 60.8
MS.gene40534.t1 AT3G48600 33.019 106 67 2 32 133 8 113 1.41e-12 62.8
MS.gene40534.t1 AT3G19080 34.783 92 57 2 40 131 250 338 1.52e-12 63.5
MS.gene40534.t1 AT3G19080 33.766 77 50 1 55 131 115 190 1.93e-11 60.5
MS.gene40534.t1 AT3G19080 34.783 92 57 2 40 131 250 338 1.52e-12 63.5
MS.gene40534.t1 AT3G19080 33.766 77 50 1 55 131 115 190 1.93e-11 60.5
MS.gene40534.t1 AT3G19080 34.783 92 57 2 40 131 250 338 1.56e-12 63.5
MS.gene40534.t1 AT3G19080 33.766 77 50 1 55 131 115 190 2.03e-11 60.5

Find 48 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCAAATGCTCTTGAGGGCT+TGG 0.201712 5.2:-381593 None:intergenic
GTGGCCTTTGCCTTCAATTA+AGG 0.272510 5.2:-382583 None:intergenic
GCTCTTGAGGGCTTGGGTTC+GGG 0.285856 5.2:-381586 None:intergenic
TGCTCTTGAGGGCTTGGGTT+CGG 0.309630 5.2:-381587 None:intergenic
CCCAAGCCCTCAAGAGCATT+TGG 0.313567 5.2:+381592 MS.gene40534:CDS
GAGCATTTGGGCCCACATTA+AGG 0.313899 5.2:+381605 MS.gene40534:CDS
CTTGGAGTCCTGAGAGTTAG+AGG 0.343205 5.2:-381402 None:intergenic
CCAAATGCTCTTGAGGGCTT+GGG 0.352861 5.2:-381592 None:intergenic
GGCTTGGGTTCGGGAAATCT+CGG 0.358689 5.2:-381577 None:intergenic
GTTGATATGCTTGAGATTGC+TGG 0.364505 5.2:+382542 MS.gene40534:CDS
GCCCTCACTTCCTTAATTGA+AGG 0.384410 5.2:+382573 MS.gene40534:CDS
CCGACGAGGTCTTGCATCTC+GGG 0.390790 5.2:-381549 None:intergenic
GCCGACGAGGTCTTGCATCT+CGG 0.410466 5.2:-381550 None:intergenic
AAACTGAAGAAGGTATTTGC+AGG 0.431242 5.2:+382509 MS.gene40534:CDS
CCAAGCCCTCAAGAGCATTT+GGG 0.438538 5.2:+381593 MS.gene40534:CDS
GCTTGGGTTCGGGAAATCTC+GGG 0.463473 5.2:-381576 None:intergenic
TCCCACACCTTCGTTGCGTA+TGG 0.464306 5.2:+381434 MS.gene40534:CDS
CATCTCCTCCTCTAACTCTC+AGG 0.478833 5.2:+381394 MS.gene40534:CDS
CATTAAGGAACACAATCTTC+AGG 0.486338 5.2:+381620 MS.gene40534:CDS
GTCTCTCAGCCTCCACCCAC+CGG 0.498374 5.2:+381486 MS.gene40534:CDS
GCCTTCAATTAAGGAAGTGA+GGG 0.502350 5.2:-382574 None:intergenic
GTGGGCCCAAATGCTCTTGA+GGG 0.503891 5.2:-381598 None:intergenic
CTCTAACTCTCAGGACTCCA+AGG 0.525205 5.2:+381403 MS.gene40534:CDS
TGTGGGCCCAAATGCTCTTG+AGG 0.532300 5.2:-381599 None:intergenic
GAAGATTGTGTTCCTTAATG+TGG 0.537334 5.2:-381617 None:intergenic
AGTGAAGATGGCGGTTTCAA+TGG 0.537531 5.2:+381353 None:intergenic
AGGAGATGATAATAATAGTC+CGG 0.546560 5.2:-381379 None:intergenic
GGTGGGTGGAGGCTGAGAGA+CGG 0.548280 5.2:-381484 None:intergenic
CGGTGGCAGAAGTAACTGTT+CGG 0.553786 5.2:-381464 None:intergenic
TGCCTTCAATTAAGGAAGTG+AGG 0.555494 5.2:-382575 None:intergenic
AAGATTGTGTTCCTTAATGT+GGG 0.565657 5.2:-381616 None:intergenic
AGCCATACGCAACGAAGGTG+TGG 0.571765 5.2:-381436 None:intergenic
GCCATACGCAACGAAGGTGT+GGG 0.575259 5.2:-381435 None:intergenic
CTGTGATGAGAAACTGAAGA+AGG 0.578111 5.2:+382499 MS.gene40534:CDS
ATGGCGGTTTCAATGGCTAC+CGG 0.582248 5.2:+381360 MS.gene40534:CDS
GGGTGGAGGCTGAGAGACGG+TGG 0.583952 5.2:-381481 None:intergenic
GGTGTGGGAAACGCAAACCT+TGG 0.584167 5.2:-381420 None:intergenic
AACTGAAGAAGGTATTTGCA+GGG 0.589332 5.2:+382510 MS.gene40534:CDS
CCCACCGGAGGCAAAATTCG+CGG 0.592965 5.2:+381501 MS.gene40534:CDS
AAATCTCGGGTTGGCCGACG+AGG 0.609292 5.2:-381563 None:intergenic
GGGTTCGGGAAATCTCGGGT+TGG 0.610231 5.2:-381572 None:intergenic
TCTCAGCCTCCACCCACCGG+AGG 0.623094 5.2:+381489 MS.gene40534:CDS
CGACGAGGTCTTGCATCTCG+GGG 0.623606 5.2:-381548 None:intergenic
CCCGAGATGCAAGACCTCGT+CGG 0.633591 5.2:+381549 MS.gene40534:CDS
GGAGTCCTGAGAGTTAGAGG+AGG 0.667796 5.2:-381399 None:intergenic
AGTGGAGCCATACGCAACGA+AGG 0.673850 5.2:-381441 None:intergenic
GACGAGGTCTTGCATCTCGG+GGG 0.684378 5.2:-381547 None:intergenic
GCAGAAGTAACTGTTCGGAG+TGG 0.693933 5.2:-381459 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAATATGAATGAATATATT+AGG + chr5.2:381891-381910 MS.gene40534:intron 15.0%
!!! TCAAAAACAAATAATTTGTA+CGG - chr5.2:381709-381728 None:intergenic 15.0%
!!! TTATTTTTAACCAACTAAAT+GGG + chr5.2:381996-382015 MS.gene40534:intron 15.0%
!!! TTTAGGTGAAATTTTAAAAA+AGG - chr5.2:381686-381705 None:intergenic 15.0%
!!! TTTATTTTTAACCAACTAAA+TGG + chr5.2:381995-382014 MS.gene40534:intron 15.0%
!! AAAAGAATGAAAACTAGATT+TGG - chr5.2:381978-381997 None:intergenic 20.0%
!! AACATGTAGTTAAGATTATA+TGG - chr5.2:382233-382252 None:intergenic 20.0%
!! AATATATTAGGCACTAATTT+GGG + chr5.2:381903-381922 MS.gene40534:intron 20.0%
!! CAAAATAAAACATGAACAAA+TGG - chr5.2:381842-381861 None:intergenic 20.0%
!! TAAGTTGTTAGTTATTAAGA+TGG + chr5.2:381761-381780 MS.gene40534:intron 20.0%
!!! TATGCAATTCTGTTTTATAA+TGG + chr5.2:382173-382192 MS.gene40534:intron 20.0%
! CATTCAATTTATGGATGATA+CGG - chr5.2:382348-382367 None:intergenic 25.0%
! GAATATATTAGGCACTAATT+TGG + chr5.2:381902-381921 MS.gene40534:intron 25.0%
! TAAACTGCATATATTCAGAA+TGG + chr5.2:381738-381757 MS.gene40534:intron 25.0%
!! ACAAATAATTTGTACGGTTT+AGG - chr5.2:381703-381722 None:intergenic 25.0%
!! ATGGATTCTATTGTCAATTT+TGG + chr5.2:382205-382224 MS.gene40534:intron 25.0%
!!! CAAAAAGTTGATGTACAAAT+TGG - chr5.2:382025-382044 None:intergenic 25.0%
AAATTGGATTCCCATTTAGT+TGG - chr5.2:382009-382028 None:intergenic 30.0%
AAGATTGTGTTCCTTAATGT+GGG - chr5.2:381619-381638 None:intergenic 30.0%
AGGAGATGATAATAATAGTC+CGG - chr5.2:381382-381401 None:intergenic 30.0%
CCATAAATTGAATGTTCTCT+GGG + chr5.2:382354-382373 MS.gene40534:intron 30.0%
TCAAACACTATACTAGTTGT+AGG - chr5.2:382319-382338 None:intergenic 30.0%
TCCATAAATTGAATGTTCTC+TGG + chr5.2:382353-382372 MS.gene40534:intron 30.0%
TGGGAATAATCGGTTATTAT+GGG + chr5.2:381922-381941 MS.gene40534:intron 30.0%
TTGGGAATAATCGGTTATTA+TGG + chr5.2:381921-381940 MS.gene40534:intron 30.0%
! TTTATAATGGTTGATGCTGA+TGG + chr5.2:382186-382205 MS.gene40534:intron 30.0%
!! TTTTGACTCAACAACCAATT+TGG + chr5.2:381857-381876 MS.gene40534:intron 30.0%
ACATAATTAACCTGTCTGTC+TGG + chr5.2:382106-382125 MS.gene40534:intron 35.0%
ATGTAGAACAAGTGCAAACA+AGG - chr5.2:382076-382095 None:intergenic 35.0%
CATTAAGGAACACAATCTTC+AGG + chr5.2:381620-381639 MS.gene40534:CDS 35.0%
CCCAGAGAACATTCAATTTA+TGG - chr5.2:382357-382376 None:intergenic 35.0%
CTGAACGTATTTATGAATGC+AGG - chr5.2:382406-382425 None:intergenic 35.0%
GAAGATTGTGTTCCTTAATG+TGG - chr5.2:381620-381639 None:intergenic 35.0%
GGAATAATCGGTTATTATGG+GGG + chr5.2:381924-381943 MS.gene40534:intron 35.0%
GGCACTAATTTGGGAATAAT+CGG + chr5.2:381912-381931 MS.gene40534:intron 35.0%
GGGAATAATCGGTTATTATG+GGG + chr5.2:381923-381942 MS.gene40534:intron 35.0%
TATCCTCAAGAGAAAGAGAA+AGG - chr5.2:381658-381677 None:intergenic 35.0%
TATGGGGGCTAAATTCAATT+GGG + chr5.2:381939-381958 MS.gene40534:intron 35.0%
TTATGAATGCAGGATAGCAT+TGG - chr5.2:382396-382415 None:intergenic 35.0%
TTATGGGGGCTAAATTCAAT+TGG + chr5.2:381938-381957 MS.gene40534:intron 35.0%
! GAATAAGCCTCTTTTTCTCA+GGG - chr5.2:382478-382497 None:intergenic 35.0%
!! AAACTGAAGAAGGTATTTGC+AGG + chr5.2:382509-382528 MS.gene40534:CDS 35.0%
!! AACTGAAGAAGGTATTTGCA+GGG + chr5.2:382510-382529 MS.gene40534:CDS 35.0%
ACTCAACAACCAATTTGGTC+TGG + chr5.2:381862-381881 MS.gene40534:intron 40.0%
CTGTGATGAGAAACTGAAGA+AGG + chr5.2:382499-382518 MS.gene40534:CDS 40.0%
GTTGATATGCTTGAGATTGC+TGG + chr5.2:382542-382561 MS.gene40534:CDS 40.0%
TACGTTCAGCAACGTTTGAT+TGG + chr5.2:382417-382436 MS.gene40534:intron 40.0%
TCTCCTTTCTCTTTCTCTTG+AGG + chr5.2:381652-381671 MS.gene40534:intron 40.0%
TGTGAGAAACCAGACCAAAT+TGG - chr5.2:381874-381893 None:intergenic 40.0%
TTCAGAACCCTGAGAAAAAG+AGG + chr5.2:382468-382487 MS.gene40534:intron 40.0%
! CGAATAAGCCTCTTTTTCTC+AGG - chr5.2:382479-382498 None:intergenic 40.0%
! GCAGGATAGCATTGGATTAA+TGG - chr5.2:382388-382407 None:intergenic 40.0%
!!! ATCTCGGGGGAGATTTTTTT+AGG - chr5.2:381537-381556 None:intergenic 40.0%
GTGCAATTGAAACGCCTTGA+TGG - chr5.2:382132-382151 None:intergenic 45.0%
! AATACCGCGAATTTTGCCTC+CGG - chr5.2:381508-381527 None:intergenic 45.0%
ATGGCGGTTTCAATGGCTAC+CGG + chr5.2:381360-381379 MS.gene40534:CDS 50.0%
CATCTCCTCCTCTAACTCTC+AGG + chr5.2:381394-381413 MS.gene40534:CDS 50.0%
CCAAATGCTCTTGAGGGCTT+GGG - chr5.2:381595-381614 None:intergenic 50.0%
CGGTGGCAGAAGTAACTGTT+CGG - chr5.2:381467-381486 None:intergenic 50.0%
CTCTAACTCTCAGGACTCCA+AGG + chr5.2:381403-381422 MS.gene40534:CDS 50.0%
CTTGGAGTCCTGAGAGTTAG+AGG - chr5.2:381405-381424 None:intergenic 50.0%
GAGCATTTGGGCCCACATTA+AGG + chr5.2:381605-381624 MS.gene40534:CDS 50.0%
GCAGAAGTAACTGTTCGGAG+TGG - chr5.2:381462-381481 None:intergenic 50.0%
!! CCAAGCCCTCAAGAGCATTT+GGG + chr5.2:381593-381612 MS.gene40534:CDS 50.0%
ACCTGTCTGTCTGGCCATCA+AGG + chr5.2:382115-382134 MS.gene40534:intron 55.0%
AGCCATACGCAACGAAGGTG+TGG - chr5.2:381439-381458 None:intergenic 55.0%
AGTGGAGCCATACGCAACGA+AGG - chr5.2:381444-381463 None:intergenic 55.0%
CCCAAATGCTCTTGAGGGCT+TGG - chr5.2:381596-381615 None:intergenic 55.0%
GCTTGGGTTCGGGAAATCTC+GGG - chr5.2:381579-381598 None:intergenic 55.0%
GGAGTCCTGAGAGTTAGAGG+AGG - chr5.2:381402-381421 None:intergenic 55.0%
GGCTTGGGTTCGGGAAATCT+CGG - chr5.2:381580-381599 None:intergenic 55.0%
GGTGTGGGAAACGCAAACCT+TGG - chr5.2:381423-381442 None:intergenic 55.0%
GTGGGCCCAAATGCTCTTGA+GGG - chr5.2:381601-381620 None:intergenic 55.0%
TCCCACACCTTCGTTGCGTA+TGG + chr5.2:381434-381453 MS.gene40534:CDS 55.0%
TGCTCTTGAGGGCTTGGGTT+CGG - chr5.2:381590-381609 None:intergenic 55.0%
TGTGGGCCCAAATGCTCTTG+AGG - chr5.2:381602-381621 None:intergenic 55.0%
! GCCATACGCAACGAAGGTGT+GGG - chr5.2:381438-381457 None:intergenic 55.0%
!! CCCAAGCCCTCAAGAGCATT+TGG + chr5.2:381592-381611 MS.gene40534:CDS 55.0%
CCCACCGGAGGCAAAATTCG+CGG + chr5.2:381501-381520 MS.gene40534:CDS 60.0%
CCCGAGATGCAAGACCTCGT+CGG + chr5.2:381549-381568 MS.gene40534:CDS 60.0%
CCGACGAGGTCTTGCATCTC+GGG - chr5.2:381552-381571 None:intergenic 60.0%
CGACGAGGTCTTGCATCTCG+GGG - chr5.2:381551-381570 None:intergenic 60.0%
GACGAGGTCTTGCATCTCGG+GGG - chr5.2:381550-381569 None:intergenic 60.0%
GCCGACGAGGTCTTGCATCT+CGG - chr5.2:381553-381572 None:intergenic 60.0%
GCTCTTGAGGGCTTGGGTTC+GGG - chr5.2:381589-381608 None:intergenic 60.0%
GGGTTCGGGAAATCTCGGGT+TGG - chr5.2:381575-381594 None:intergenic 60.0%
! ACCGCGAATTTTGCCTCCGG+TGG - chr5.2:381505-381524 None:intergenic 60.0%
! ATTTTGCCTCCGGTGGGTGG+AGG - chr5.2:381498-381517 None:intergenic 60.0%
! CCGCGAATTTTGCCTCCGGT+GGG - chr5.2:381504-381523 None:intergenic 60.0%
! CGAATTTTGCCTCCGGTGGG+TGG - chr5.2:381501-381520 None:intergenic 60.0%
! GCCTTGATGGCCAGACAGAC+AGG - chr5.2:382119-382138 None:intergenic 60.0%
!! AAATCTCGGGTTGGCCGACG+AGG - chr5.2:381566-381585 None:intergenic 60.0%
GGTGGGTGGAGGCTGAGAGA+CGG - chr5.2:381487-381506 None:intergenic 65.0%
GTCTCTCAGCCTCCACCCAC+CGG + chr5.2:381486-381505 MS.gene40534:CDS 65.0%
GGGTGGAGGCTGAGAGACGG+TGG - chr5.2:381484-381503 None:intergenic 70.0%
TCTCAGCCTCCACCCACCGG+AGG + chr5.2:381489-381508 MS.gene40534:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 381360 382592 381360 ID=MS.gene40534
chr5.2 mRNA 381360 382592 381360 ID=MS.gene40534.t1;Parent=MS.gene40534
chr5.2 exon 381360 381641 381360 ID=MS.gene40534.t1.exon1;Parent=MS.gene40534.t1
chr5.2 CDS 381360 381641 381360 ID=cds.MS.gene40534.t1;Parent=MS.gene40534.t1
chr5.2 exon 382473 382592 382473 ID=MS.gene40534.t1.exon2;Parent=MS.gene40534.t1
chr5.2 CDS 382473 382592 382473 ID=cds.MS.gene40534.t1;Parent=MS.gene40534.t1
Gene Sequence

>MS.gene40534

ATGGCGGTTTCAATGGCTACCGGACTATTATTATCATCTCCTCCTCTAACTCTCAGGACTCCAAGGTTTGCGTTTCCCACACCTTCGTTGCGTATGGCTCCACTCCGAACAGTTACTTCTGCCACCGTCTCTCAGCCTCCACCCACCGGAGGCAAAATTCGCGGTATTATGAAACCTAAAAAAATCTCCCCCGAGATGCAAGACCTCGTCGGCCAACCCGAGATTTCCCGAACCCAAGCCCTCAAGAGCATTTGGGCCCACATTAAGGAACACAATCTTCAGGTCTAACTTCTCTCCTTTCTCTTTCTCTTGAGGATAATAAGCCTTTTTTAAAATTTCACCTAAACCGTACAAATTATTTGTTTTTGAAATTGTTGTTAAACTGCATATATTCAGAATGGTAAGTTGTTAGTTATTAAGATGGTTTCATTTGAAGACATGATATCTGTTTCATTGACTACAATACATATATTACAAGACCATTTGTTCATGTTTTATTTTGACTCAACAACCAATTTGGTCTGGTTTCTCACAATATGAATGAATATATTAGGCACTAATTTGGGAATAATCGGTTATTATGGGGGCTAAATTCAATTGGGCTGCAGCAGAAAACCAAATCTAGTTTTCATTCTTTTATTTTTAACCAACTAAATGGGAATCCAATTTGTACATCAACTTTTTGACTGTCATATCTAACTCTGTATTTTTAACCTTGTTTGCACTTGTTCTACATACAGCACAATACATAATTAACCTGTCTGTCTGGCCATCAAGGCGTTTCAATTGCACAAATTTTCTGAATTTGTTGTATATGCAATTCTGTTTTATAATGGTTGATGCTGATGGATTCTATTGTCAATTTTGGAGCCATATAATCTTAACTACATGTTTCATATAATTAGATTACAATTCAAGTGTTTATTATTATTATTATTATTATTATTTTCTAGCTTCCTACAACTAGTATAGTGTTTGATTATTTCCGTATCATCCATAAATTGAATGTTCTCTGGGATAATTATCCATTAATCCAATGCTATCCTGCATTCATAAATACGTTCAGCAACGTTTGATTGGTTTTAATTGAATGTTTCTTTCTAATATATTCAGAACCCTGAGAAAAAGAGGCTTATTCGCTGTGATGAGAAACTGAAGAAGGTATTTGCAGGGAGAGATGAAGTTGATATGCTTGAGATTGCTGGCTTGATTAGCCCTCACTTCCTTAATTGA

Protein sequence

>MS.gene40534.t1

MAVSMATGLLLSSPPLTLRTPRFAFPTPSLRMAPLRTVTSATVSQPPPTGGKIRGIMKPKKISPEMQDLVGQPEISRTQALKSIWAHIKEHNLQNPEKKRLIRCDEKLKKVFAGRDEVDMLEIAGLISPHFLN