AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007465.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007465.01.T01 MTR_2g426020 79.703 404 23 2 1 352 1 397 0.0 642
MsG0280007465.01.T01 MTR_2g426060 64.524 420 82 7 1 364 1 409 2.73e-177 498
MsG0280007465.01.T01 MTR_2g025420 60.187 427 104 8 1 364 1 424 3.56e-165 468
MsG0280007465.01.T01 MTR_2g425980 60.533 413 94 7 1 356 1 401 1.37e-156 446
MsG0280007465.01.T01 MTR_2g426070 59.759 415 93 9 1 355 1 401 8.72e-156 444
MsG0280007465.01.T01 MTR_3g083010 60.993 423 91 13 1 355 1 417 2.92e-154 440
MsG0280007465.01.T01 MTR_2g028160 60.140 429 97 13 1 364 1 420 8.11e-149 427
MsG0280007465.01.T01 MTR_0667s0010 57.820 422 107 10 1 364 1 409 2.07e-148 425
MsG0280007465.01.T01 MTR_2g028140 56.177 429 112 9 1 364 1 418 9.40e-143 411
MsG0280007465.01.T01 MTR_2g028130 58.173 416 98 11 1 350 1 406 9.87e-143 411
MsG0280007465.01.T01 MTR_2g426090 56.075 428 108 9 1 364 1 412 4.12e-142 409
MsG0280007465.01.T01 MTR_2g024100 56.234 393 107 8 21 357 22 405 5.71e-130 378
MsG0280007465.01.T01 MTR_2g028110 53.283 396 111 6 6 350 4 376 6.09e-127 370
MsG0280007465.01.T01 MTR_2g021050 56.234 393 117 10 1 352 1 379 1.54e-126 369
MsG0280007465.01.T01 MTR_4g070550 53.162 427 113 13 1 364 1 403 1.51e-123 362
MsG0280007465.01.T01 MTR_8g099460 52.582 426 117 11 1 364 1 403 2.36e-119 351
MsG0280007465.01.T01 MTR_1g068835 51.683 416 106 13 6 364 4 381 1.15e-116 343
MsG0280007465.01.T01 MTR_2g031720 49.764 424 125 12 1 364 1 396 7.88e-113 334
MsG0280007465.01.T01 MTR_1g068875 51.923 416 104 14 6 364 4 380 2.40e-112 332
MsG0280007465.01.T01 MTR_2g031640 51.759 398 128 14 1 352 1 380 2.17e-107 320
MsG0280007465.01.T01 MTR_2g025960 55.732 314 75 4 96 352 1 307 3.42e-107 317
MsG0280007465.01.T01 MTR_2g031680 48.210 419 132 11 1 364 1 389 1.60e-105 315
MsG0280007465.01.T01 MTR_2g026010 48.946 427 118 12 6 364 4 398 3.84e-103 310
MsG0280007465.01.T01 MTR_1g068860 50.868 403 105 13 6 350 4 371 7.78e-103 308
MsG0280007465.01.T01 MTR_2g024110 45.547 393 128 11 6 338 4 370 4.96e-92 280
MsG0280007465.01.T01 MTR_1g068905 44.271 384 94 12 6 350 4 306 1.01e-78 244
MsG0280007465.01.T01 MTR_4g006270 40.201 398 160 10 6 353 4 373 1.50e-74 236
MsG0280007465.01.T01 MTR_7g110360 44.444 342 117 11 6 334 39 320 3.83e-68 217
MsG0280007465.01.T01 MTR_4g133170 34.917 421 180 10 6 364 4 392 1.70e-64 210
MsG0280007465.01.T01 MTR_2g037520 66.667 156 44 2 166 319 2 151 1.91e-64 202
MsG0280007465.01.T01 MTR_2g031700 41.854 356 144 10 1 356 1 293 9.25e-61 197
MsG0280007465.01.T01 MTR_2g025440 72.519 131 33 2 222 350 1 130 4.82e-60 193
MsG0280007465.01.T01 MTR_5g083940 35.928 334 126 13 6 314 4 274 5.20e-41 147
MsG0280007465.01.T01 MTR_3g086960 50.625 160 50 6 189 335 10 153 7.47e-35 125
MsG0280007465.01.T01 MTR_3g014570 25.587 383 199 13 6 333 29 380 1.99e-28 114
MsG0280007465.01.T01 MTR_3g014540 26.142 394 182 14 6 333 29 379 3.19e-28 114
MsG0280007465.01.T01 MTR_7g110370 65.556 90 24 3 199 286 13 97 2.00e-27 108
MsG0280007465.01.T01 MTR_2g022170 47.407 135 52 4 9 142 7 123 2.12e-26 102
MsG0280007465.01.T01 MTR_2g040050 36.000 225 96 10 89 277 29 241 2.53e-25 103
MsG0280007465.01.T01 MTR_3g010440 27.114 343 198 12 6 332 26 332 2.23e-24 102
MsG0280007465.01.T01 MTR_2g046900 27.132 387 197 13 6 334 28 387 3.53e-23 99.8
MsG0280007465.01.T01 MTR_3g006670 25.743 404 181 15 6 332 49 410 9.99e-23 99.0
MsG0280007465.01.T01 MTR_2g028120 61.250 80 22 3 274 352 7 78 1.06e-22 90.9
MsG0280007465.01.T01 MTR_2g021150 28.760 379 195 16 6 332 14 369 2.44e-22 97.4
MsG0280007465.01.T01 MTR_5g046300 24.339 378 205 12 6 332 4 351 5.76e-22 95.9
MsG0280007465.01.T01 MTR_5g046290 24.339 378 205 12 6 332 4 351 5.76e-22 95.9
MsG0280007465.01.T01 MTR_3g011100 25.823 395 198 15 6 332 235 602 1.77e-21 96.3
MsG0280007465.01.T01 MTR_3g010390 27.750 400 182 16 6 332 33 398 3.52e-21 94.4
MsG0280007465.01.T01 MTR_3g013740 26.098 387 203 15 6 332 44 407 3.64e-20 91.7
MsG0280007465.01.T01 MTR_8g054320 28.135 327 178 9 6 332 32 301 3.85e-20 90.1
MsG0280007465.01.T01 MTR_3g007330 26.302 384 192 15 6 334 48 395 4.27e-20 91.3
MsG0280007465.01.T01 MTR_3g010920 26.374 364 206 14 6 332 44 382 5.30e-20 90.9
MsG0280007465.01.T01 MTR_7g407070 25.688 327 152 11 6 262 32 337 5.32e-20 90.9
MsG0280007465.01.T01 MTR_3g013770 25.849 383 203 13 6 333 33 389 5.93e-20 90.5
MsG0280007465.01.T01 MTR_0760s0010 28.767 365 195 19 11 334 52 392 8.69e-20 90.1
MsG0280007465.01.T01 MTR_2g025995 86.792 53 6 1 1 52 1 53 1.09e-19 87.8
MsG0280007465.01.T01 MTR_2g021155 27.507 349 166 13 9 298 39 359 2.47e-19 90.1
MsG0280007465.01.T01 MTR_4g055060 26.463 393 192 15 6 334 30 389 3.01e-19 88.6
MsG0280007465.01.T01 MTR_3g009240 27.979 386 181 19 6 332 36 383 3.49e-19 88.2
MsG0280007465.01.T01 MTR_3g009310 25.459 381 199 16 6 332 42 391 3.86e-19 88.2
MsG0280007465.01.T01 MTR_1g055165 26.420 352 202 12 2 338 6 315 6.58e-19 86.7
MsG0280007465.01.T01 MTR_2g046920 26.273 373 210 13 6 334 24 375 1.85e-18 86.3
MsG0280007465.01.T01 MTR_3g036290 25.936 374 196 14 6 334 22 359 3.30e-18 85.1
MsG0280007465.01.T01 MTR_7g022670 28.611 360 180 15 3 316 20 348 3.54e-18 85.1
MsG0280007465.01.T01 MTR_3g058470 27.838 370 194 17 6 334 37 374 4.19e-18 85.1
MsG0280007465.01.T01 MTR_3g007300 26.446 363 191 16 6 334 49 369 4.68e-18 84.7
MsG0280007465.01.T01 MTR_1g097560 24.800 375 206 14 3 333 33 375 9.34e-18 84.0
MsG0280007465.01.T01 MTR_3g011020 26.415 371 202 13 6 332 28 371 1.69e-17 83.2
MsG0280007465.01.T01 MTR_5g011200 25.773 388 204 16 3 332 14 375 6.34e-17 81.6
MsG0280007465.01.T01 MTR_3g030330 25.199 377 208 15 6 332 20 372 7.35e-17 81.3
MsG0280007465.01.T01 MTR_6g021880 26.862 376 191 14 17 332 31 382 8.02e-17 81.3
MsG0280007465.01.T01 MTR_3g011060 26.209 393 191 18 6 332 28 387 1.33e-16 80.9
MsG0280007465.01.T01 MTR_3g036335 24.289 387 201 16 6 332 41 395 1.54e-16 80.5
MsG0280007465.01.T01 MTR_2g046830 25.843 356 196 13 9 318 28 361 1.75e-16 80.5
MsG0280007465.01.T01 MTR_3g436150 26.994 326 143 10 6 262 32 331 2.66e-16 80.1
MsG0280007465.01.T01 MTR_0044s0300 24.806 387 197 17 6 332 38 390 3.46e-16 79.7
MsG0280007465.01.T01 MTR_1g097330 27.794 349 194 17 6 314 16 346 1.04e-15 78.2
MsG0280007465.01.T01 MTR_2g078850 25.356 351 182 16 9 306 33 356 2.26e-15 77.0
MsG0280007465.01.T01 MTR_3g009260 25.729 377 198 13 6 332 41 385 4.32e-15 76.3
MsG0280007465.01.T01 MTR_5g009060 30.636 173 94 7 166 334 251 401 4.76e-15 76.3
MsG0280007465.01.T01 MTR_7g056737 26.814 317 150 13 6 262 22 316 6.18e-15 75.9
MsG0280007465.01.T01 MTR_3g010970 25.691 362 211 11 9 332 51 392 6.41e-15 75.9
MsG0280007465.01.T01 MTR_3g009300 26.849 365 182 16 9 334 37 355 1.13e-14 74.7
MsG0280007465.01.T01 MTR_3g024040 25.326 383 182 15 12 334 37 375 1.37e-14 74.7
MsG0280007465.01.T01 MTR_5g057710 24.742 388 193 15 6 332 46 395 1.86e-14 74.3
MsG0280007465.01.T01 MTR_3g010590 25.197 381 198 15 6 332 39 386 2.42e-14 73.9
MsG0280007465.01.T01 MTR_3g030350 23.670 376 219 11 6 332 35 391 3.23e-14 73.6
MsG0280007465.01.T01 MTR_2g021210 24.828 290 168 10 6 260 84 358 2.03e-13 71.6
MsG0280007465.01.T01 MTR_3g024130 23.975 317 153 12 72 332 31 315 6.96e-13 68.9
MsG0280007465.01.T01 MTR_3g096250 26.493 268 163 12 6 262 47 291 7.15e-13 69.3
MsG0280007465.01.T01 MTR_3g006500 24.289 387 200 15 6 332 17 370 7.75e-13 69.3
MsG0280007465.01.T01 MTR_8g064790 24.390 287 164 11 9 268 18 278 1.82e-12 68.2
MsG0280007465.01.T01 MTR_1g055055 26.409 337 185 18 6 306 26 335 4.74e-12 66.6
MsG0280007465.01.T01 MTR_7g056200 23.669 338 178 14 6 286 23 337 6.44e-12 66.6
MsG0280007465.01.T01 MTR_7g079160 29.444 180 104 5 124 290 215 384 6.77e-12 67.0
MsG0280007465.01.T01 MTR_7g035295 22.165 388 225 13 6 329 16 390 6.97e-12 66.6
MsG0280007465.01.T01 MTR_5g056990 31.361 169 96 7 166 332 90 240 2.78e-11 63.5
MsG0280007465.01.T01 MTR_6g088385 23.512 336 207 12 6 331 23 318 3.87e-11 63.9
MsG0280007465.01.T01 MTR_1004s0010 28.000 175 104 8 164 334 206 362 7.27e-11 63.2
MsG0280007465.01.T01 MTR_7g077070 32.857 140 80 3 6 131 16 155 8.77e-11 63.5
MsG0280007465.01.T01 MTR_4g061340 25.000 228 124 9 107 332 194 376 8.78e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007465.01.T01 AT4G12560 28.571 434 211 14 6 364 4 413 7.03e-40 146
MsG0280007465.01.T01 AT4G12560 28.571 434 211 14 6 364 4 413 7.03e-40 146
MsG0280007465.01.T01 AT4G12560 28.571 434 211 14 6 364 4 413 7.03e-40 146
MsG0280007465.01.T01 AT3G06240 23.501 417 199 12 6 333 36 421 1.02e-19 90.5
MsG0280007465.01.T01 AT4G22390 32.692 208 118 7 164 364 209 401 1.17e-19 90.1
MsG0280007465.01.T01 AT4G22390 31.579 209 120 7 164 364 209 402 2.07e-18 86.3

Find 91 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAGCTTCATGCCTCTTAT+TGG 0.188781 2:-15850740 MsG0280007465.01.T01:CDS
TGTTGAAGGAATTCCTAATT+TGG 0.248257 2:-15849927 MsG0280007465.01.T01:CDS
GAATTTGTGTCGAATGATTA+AGG 0.255657 2:+15850856 None:intergenic
AAGATTTCGGCGAGTATTTC+CGG 0.298990 2:+15850995 None:intergenic
TTCAAGATTGTGATTTCATT+AGG 0.302272 2:+15850656 None:intergenic
TTCTTCCTACAATTATCAAC+AGG 0.317221 2:+15849860 None:intergenic
CCTCTTATTGGTTCATTTAA+CGG 0.318117 2:-15850728 MsG0280007465.01.T01:CDS
CGTGAAGCAGATTTGGCTAT+TGG 0.318441 2:+15850691 None:intergenic
CCTTCCCTGTTGATAATTGT+AGG 0.327370 2:-15849865 MsG0280007465.01.T01:CDS
TCAAGATTGTGATTTCATTA+GGG 0.330378 2:+15850657 None:intergenic
ATCATAGCTTCATCCAAATT+AGG 0.332948 2:+15849914 None:intergenic
TAAATCATAGGAAGCTGTTT+TGG 0.344810 2:-15849979 MsG0280007465.01.T01:CDS
TTCCGATGTTTATGAGTGTT+AGG 0.346588 2:+15850632 None:intergenic
TGGTAAGCGGGACAAGATTT+CGG 0.349156 2:+15850982 None:intergenic
AAGGATGCGTGAAGCAGATT+TGG 0.367915 2:+15850684 None:intergenic
GGGATAGGAAGTGGGAGAAA+AGG 0.374308 2:+15850605 None:intergenic
CGCGCCGGTGTATGCGTTCA+CGG 0.375752 2:-15850554 MsG0280007465.01.T01:CDS
GGATTTAAATTGAGATTGTA+AGG 0.380153 2:-15849437 None:intergenic
GAAGTTATGAGAATCGATTA+TGG 0.385608 2:+15850913 None:intergenic
CATTGAAAGTCTCAAGTGTT+AGG 0.386620 2:+15850284 None:intergenic
GGATAGGAAGTGGGAGAAAA+GGG 0.393677 2:+15850606 None:intergenic
ATGAAGCTGGTACGCGTGAT+AGG 0.405010 2:+15850752 None:intergenic
TTGTTGAGAATTCTATTCAC+TGG 0.416089 2:-15850360 MsG0280007465.01.T01:CDS
ACTTCCTATCCCTAACATTC+TGG 0.416990 2:-15850594 MsG0280007465.01.T01:CDS
ATTTAGAAGTTGATCGGAAT+CGG 0.421238 2:+15850945 None:intergenic
GAACAAATTAGTTATGTTGA+AGG 0.424344 2:-15849941 MsG0280007465.01.T01:CDS
GGTTCACTTTGAAATCTTTG+AGG 0.429304 2:-15850066 MsG0280007465.01.T01:CDS
GCAGATTTGGCTATTGGAAA+TGG 0.429389 2:+15850697 None:intergenic
CCTCTTCCTGCTGAAATAGA+AGG 0.430840 2:-15850256 MsG0280007465.01.T01:CDS
GTGTGGGTGATGAAAGAATA+TGG 0.432977 2:-15850133 MsG0280007465.01.T01:CDS
GGTGTATGCGTTCACGGCTT+CGG 0.433344 2:-15850548 MsG0280007465.01.T01:intron
CCATCCCTCGAATAACATAA+AGG 0.444053 2:+15850043 None:intergenic
ACAATCTCAATTTAAATCCT+CGG 0.447130 2:+15849441 None:intergenic
TCCGATGTTTATGAGTGTTA+GGG 0.457303 2:+15850633 None:intergenic
CAATCTCAATTTAAATCCTC+GGG 0.460965 2:+15849442 None:intergenic
TTATTCGAGGGATGGAAGTA+AGG 0.465310 2:-15850035 MsG0280007465.01.T01:CDS
TGAGGCCTTTATGTTATTCG+AGG 0.467945 2:-15850048 MsG0280007465.01.T01:CDS
CTTCAATATGTTCAGGCCAT+GGG 0.467982 2:-15850387 MsG0280007465.01.T01:intron
TTTCAGACTCCAGAATGTTA+GGG 0.484648 2:+15850585 None:intergenic
TCTTCACCTTCTATTTCAGC+AGG 0.487406 2:+15850250 None:intergenic
CCTTCTATTTCAGCAGGAAG+AGG 0.490143 2:+15850256 None:intergenic
GTGATTTCATTAGGGGCGTA+AGG 0.497760 2:+15850665 None:intergenic
GGCGGCGGACCGCCTTCCGC+CGG 0.498521 2:-15851014 MsG0280007465.01.T01:CDS
GTGATAGGAGCGGTCTTGAA+AGG 0.503652 2:+15850767 None:intergenic
GGTTCTGTTGGACGGAACTC+AGG 0.512293 2:-15850014 MsG0280007465.01.T01:CDS
AACCTTCTTTCCGATGGCGG+CGG 0.512297 2:-15851029 None:intergenic
TAAGTGATTTAGAAGTTGAT+CGG 0.515656 2:+15850939 None:intergenic
AGAATGTTAGGGATAGGAAG+TGG 0.516803 2:+15850596 None:intergenic
GGATGGAAGTAAGGTTCTGT+TGG 0.517080 2:-15850026 MsG0280007465.01.T01:CDS
CATCATCATCGATTTGGAGT+TGG 0.522312 2:+15850825 None:intergenic
CGGCGAGTATTTCCGGCGGA+AGG 0.526423 2:+15851002 None:intergenic
ACCAATAAGAGGCATGAAGC+TGG 0.532873 2:+15850739 None:intergenic
CTATTCACTGGGTAATGACT+CGG 0.533868 2:-15850348 MsG0280007465.01.T01:CDS
AAGTGATTGAACTGGTAAGC+GGG 0.534461 2:+15850970 None:intergenic
CCGTTAAATGAACCAATAAG+AGG 0.536060 2:+15850728 None:intergenic
CCTTTATGTTATTCGAGGGA+TGG 0.537981 2:-15850043 MsG0280007465.01.T01:CDS
TGTTGATAATTGTAGGAAGA+AGG 0.538310 2:-15849858 MsG0280007465.01.T01:CDS
AATCGGAGAAGTGATTGAAC+TGG 0.540452 2:+15850962 None:intergenic
GGAAGGCGGTCCGCCGCCAT+CGG 0.546682 2:+15851019 None:intergenic
TGTTGAGAATTCTATTCACT+GGG 0.546900 2:-15850359 MsG0280007465.01.T01:CDS
GAGGCCTTTATGTTATTCGA+GGG 0.547661 2:-15850047 MsG0280007465.01.T01:CDS
CAAGATTGTGATTTCATTAG+GGG 0.556293 2:+15850658 None:intergenic
TTCAATATGTTCAGGCCATG+GGG 0.558081 2:-15850386 MsG0280007465.01.T01:intron
AACTACTAAAATTGATGTGT+GGG 0.576733 2:-15850149 MsG0280007465.01.T01:CDS
GACTCCAGAATGTTAGGGAT+AGG 0.576814 2:+15850590 None:intergenic
GATTTAAATTGAGATTGTAA+GGG 0.578977 2:-15849436 None:intergenic
CAATTATCAACAGGGAAGGA+AGG 0.581752 2:+15849869 None:intergenic
AATATGGTTCTAGAGATAGT+TGG 0.584921 2:-15850117 MsG0280007465.01.T01:CDS
CGAGTATTTCCGGCGGAAGG+CGG 0.585910 2:+15851005 None:intergenic
TAGGAGCGGTCTTGAAAGGA+TGG 0.597121 2:+15850771 None:intergenic
GGAAGTAAGGTTCTGTTGGA+CGG 0.599701 2:-15850022 MsG0280007465.01.T01:CDS
ATACAAACAAGAGAAAGAAA+CGG 0.600263 2:-15849829 MsG0280007465.01.T01:intron
AGGGAGGACTTCTTGTCCCG+AGG 0.609923 2:-15849458 MsG0280007465.01.T01:CDS
CCTACAATTATCAACAGGGA+AGG 0.622074 2:+15849865 None:intergenic
AAACTACTAAAATTGATGTG+TGG 0.624349 2:-15850150 MsG0280007465.01.T01:CDS
GAATGTTAGGGATAGGAAGT+GGG 0.628949 2:+15850597 None:intergenic
GTCTTGAAAGGATGGTTGAG+TGG 0.634678 2:+15850779 None:intergenic
TTTGGCTATTGGAAATGGCG+AGG 0.634701 2:+15850702 None:intergenic
TCAATATGTTCAGGCCATGG+GGG 0.636969 2:-15850385 MsG0280007465.01.T01:intron
ACCCTAACACTCATAAACAT+CGG 0.641945 2:-15850634 MsG0280007465.01.T01:CDS
AACACTTGAGACTTTCAATG+TGG 0.646577 2:-15850281 MsG0280007465.01.T01:CDS
TCTGAAAACATTGATCGCGC+CGG 0.650592 2:-15850569 MsG0280007465.01.T01:CDS
GGCTATTGGAAATGGCGAGG+AGG 0.651134 2:+15850705 None:intergenic
GAAGTGATTGAACTGGTAAG+CGG 0.656074 2:+15850969 None:intergenic
GCTGGTACGCGTGATAGGAG+CGG 0.660027 2:+15850757 None:intergenic
TTATCAACAGGGAAGGAAGG+TGG 0.663317 2:+15849872 None:intergenic
TTAAACGCGACAATGAAACA+AGG 0.667325 2:+15850307 None:intergenic
GAAGCCGTGAACGCATACAC+CGG 0.672399 2:+15850550 None:intergenic
TCTTCCTACAATTATCAACA+GGG 0.677615 2:+15849861 None:intergenic
TAAACGCGACAATGAAACAA+GGG 0.707857 2:+15850308 None:intergenic
ATTTCGGCGAGTATTTCCGG+CGG 0.777904 2:+15850998 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAATTTGAATTCTTATTAT+AGG - Chr2:15850756-15850775 MsG0280007465.01.T01:CDS 15.0%
!!! ATTCATTTTTTTTTTCAACA+GGG - Chr2:15850978-15850997 MsG0280007465.01.T01:CDS 15.0%
!! AACTACATATATCATATGAT+GGG + Chr2:15850719-15850738 None:intergenic 20.0%
!! GGTTGACAAAATAATTATTT+AGG + Chr2:15850926-15850945 None:intergenic 20.0%
!! TAACTACATATATCATATGA+TGG + Chr2:15850720-15850739 None:intergenic 20.0%
!!! GATTCATTTTTTTTTTCAAC+AGG - Chr2:15850977-15850996 MsG0280007465.01.T01:CDS 20.0%
! AAACTACTAAAATTGATGTG+TGG - Chr2:15850305-15850324 MsG0280007465.01.T01:CDS 25.0%
! AACTACTAAAATTGATGTGT+GGG - Chr2:15850306-15850325 MsG0280007465.01.T01:CDS 25.0%
! ACAATCTCAATTTAAATCCT+CGG + Chr2:15851017-15851036 None:intergenic 25.0%
! ATACAAACAAGAGAAAGAAA+CGG - Chr2:15850626-15850645 MsG0280007465.01.T01:CDS 25.0%
! GAACAAATTAGTTATGTTGA+AGG - Chr2:15850514-15850533 MsG0280007465.01.T01:intron 25.0%
! TCAAGATTGTGATTTCATTA+GGG + Chr2:15849801-15849820 None:intergenic 25.0%
! TGAATTCTTATTATAGGACA+AGG - Chr2:15850762-15850781 MsG0280007465.01.T01:CDS 25.0%
! TTCAAGATTGTGATTTCATT+AGG + Chr2:15849802-15849821 None:intergenic 25.0%
!! ATCTATTGATAGAGTTTTTG+AGG + Chr2:15849579-15849598 None:intergenic 25.0%
!! TAAGTGATTTAGAAGTTGAT+CGG + Chr2:15849519-15849538 None:intergenic 25.0%
!! TTGGTGTAAACTTTTTACTT+TGG - Chr2:15850357-15850376 MsG0280007465.01.T01:CDS 25.0%
!! TTTTTACTTTGGATAAATCG+CGG - Chr2:15850368-15850387 MsG0280007465.01.T01:CDS 25.0%
!!! TATTTTCCATGAGTTTGTTT+TGG + Chr2:15850028-15850047 None:intergenic 25.0%
ATCATAGCTTCATCCAAATT+AGG + Chr2:15850544-15850563 None:intergenic 30.0%
CAAGATTGTGATTTCATTAG+GGG + Chr2:15849800-15849819 None:intergenic 30.0%
CAATCTCAATTTAAATCCTC+GGG + Chr2:15851016-15851035 None:intergenic 30.0%
GAAGTTATGAGAATCGATTA+TGG + Chr2:15849545-15849564 None:intergenic 30.0%
GAATTTGTGTCGAATGATTA+AGG + Chr2:15849602-15849621 None:intergenic 30.0%
GTAGTTAAACTCTATGCTAA+AGG - Chr2:15850733-15850752 MsG0280007465.01.T01:CDS 30.0%
TCAACAATATCACACAAGAA+TGG + Chr2:15850947-15850966 None:intergenic 30.0%
TCTTCCTACAATTATCAACA+GGG + Chr2:15850597-15850616 None:intergenic 30.0%
TGTTGAAGGAATTCCTAATT+TGG - Chr2:15850528-15850547 MsG0280007465.01.T01:intron 30.0%
TGTTGAGAATTCTATTCACT+GGG - Chr2:15850096-15850115 MsG0280007465.01.T01:CDS 30.0%
TTCTTCCTACAATTATCAAC+AGG + Chr2:15850598-15850617 None:intergenic 30.0%
TTGTTGAGAATTCTATTCAC+TGG - Chr2:15850095-15850114 MsG0280007465.01.T01:CDS 30.0%
! AATCATCATCATCATCGATT+TGG + Chr2:15849639-15849658 None:intergenic 30.0%
! ATTTAGAAGTTGATCGGAAT+CGG + Chr2:15849513-15849532 None:intergenic 30.0%
! CTAAGTTAATGTCACTTCAA+TGG - Chr2:15850692-15850711 MsG0280007465.01.T01:CDS 30.0%
! TAAATCATAGGAAGCTGTTT+TGG - Chr2:15850476-15850495 MsG0280007465.01.T01:intron 30.0%
! TTTTGTTGGAGGTAAATCAT+AGG - Chr2:15850464-15850483 MsG0280007465.01.T01:intron 30.0%
!! AATATGGTTCTAGAGATAGT+TGG - Chr2:15850338-15850357 MsG0280007465.01.T01:CDS 30.0%
!! CCTCTTATTGGTTCATTTAA+CGG - Chr2:15849727-15849746 MsG0280007465.01.T01:intron 30.0%
!! TGTTGATAATTGTAGGAAGA+AGG - Chr2:15850597-15850616 MsG0280007465.01.T01:CDS 30.0%
!!! CATTTTTTTTTTCAACAGGG+AGG - Chr2:15850981-15851000 MsG0280007465.01.T01:CDS 30.0%
AACACTTGAGACTTTCAATG+TGG - Chr2:15850174-15850193 MsG0280007465.01.T01:CDS 35.0%
ACAAAATCCTCAGAATCTCT+TGG - Chr2:15849953-15849972 MsG0280007465.01.T01:CDS 35.0%
ACCCTAACACTCATAAACAT+CGG - Chr2:15849821-15849840 MsG0280007465.01.T01:intron 35.0%
ATTTAGGAGTGAAGATCTCA+AGG + Chr2:15850910-15850929 None:intergenic 35.0%
CATTGAAAGTCTCAAGTGTT+AGG + Chr2:15850174-15850193 None:intergenic 35.0%
CCGTTAAATGAACCAATAAG+AGG + Chr2:15849730-15849749 None:intergenic 35.0%
GTGGATCAAAAGAGTAATGT+AGG + Chr2:15849987-15850006 None:intergenic 35.0%
TAAACGCGACAATGAAACAA+GGG + Chr2:15850150-15850169 None:intergenic 35.0%
TCCGATGTTTATGAGTGTTA+GGG + Chr2:15849825-15849844 None:intergenic 35.0%
TTAAACGCGACAATGAAACA+AGG + Chr2:15850151-15850170 None:intergenic 35.0%
TTCCGATGTTTATGAGTGTT+AGG + Chr2:15849826-15849845 None:intergenic 35.0%
TTCGGTTATGATCCATTAAC+CGG - Chr2:15849925-15849944 MsG0280007465.01.T01:CDS 35.0%
TTTCAGACTCCAGAATGTTA+GGG + Chr2:15849873-15849892 None:intergenic 35.0%
! ACATTACTCTTTTGATCCAC+AGG - Chr2:15849987-15850006 MsG0280007465.01.T01:CDS 35.0%
! TTTTCAGACTCCAGAATGTT+AGG + Chr2:15849874-15849893 None:intergenic 35.0%
!! ATTTTGTAATCGCCGGTTAA+TGG + Chr2:15849940-15849959 None:intergenic 35.0%
!! GGTTCACTTTGAAATCTTTG+AGG - Chr2:15850389-15850408 MsG0280007465.01.T01:intron 35.0%
!!! ACAATTGCTGTTGCTGTTTT+AGG - Chr2:15850253-15850272 MsG0280007465.01.T01:CDS 35.0%
AAGATTTCGGCGAGTATTTC+CGG + Chr2:15849463-15849482 None:intergenic 40.0%
AAGTGATTGAACTGGTAAGC+GGG + Chr2:15849488-15849507 None:intergenic 40.0%
AATCGGAGAAGTGATTGAAC+TGG + Chr2:15849496-15849515 None:intergenic 40.0%
AATTCTCAACAAAACCCCCA+TGG + Chr2:15850087-15850106 None:intergenic 40.0%
ACTTCCTATCCCTAACATTC+TGG - Chr2:15849861-15849880 MsG0280007465.01.T01:CDS 40.0%
AGAATGTTAGGGATAGGAAG+TGG + Chr2:15849862-15849881 None:intergenic 40.0%
ATGTGACAATGTGTGTATGC+TGG - Chr2:15850826-15850845 MsG0280007465.01.T01:CDS 40.0%
CAATTATCAACAGGGAAGGA+AGG + Chr2:15850589-15850608 None:intergenic 40.0%
CCATCCCTCGAATAACATAA+AGG + Chr2:15850415-15850434 None:intergenic 40.0%
CCTACAATTATCAACAGGGA+AGG + Chr2:15850593-15850612 None:intergenic 40.0%
CCTTTATGTTATTCGAGGGA+TGG - Chr2:15850412-15850431 MsG0280007465.01.T01:intron 40.0%
CTATTCACTGGGTAATGACT+CGG - Chr2:15850107-15850126 MsG0280007465.01.T01:CDS 40.0%
CTTCAATATGTTCAGGCCAT+GGG - Chr2:15850068-15850087 MsG0280007465.01.T01:CDS 40.0%
GAAGTGATTGAACTGGTAAG+CGG + Chr2:15849489-15849508 None:intergenic 40.0%
GAATGTTAGGGATAGGAAGT+GGG + Chr2:15849861-15849880 None:intergenic 40.0%
GAGGCCTTTATGTTATTCGA+GGG - Chr2:15850408-15850427 MsG0280007465.01.T01:intron 40.0%
GCAGATTTGGCTATTGGAAA+TGG + Chr2:15849761-15849780 None:intergenic 40.0%
GTGTGGGTGATGAAAGAATA+TGG - Chr2:15850322-15850341 MsG0280007465.01.T01:CDS 40.0%
TCAGCTCCAAAACAAACTCA+TGG - Chr2:15850019-15850038 MsG0280007465.01.T01:CDS 40.0%
TCTTCACCTTCTATTTCAGC+AGG + Chr2:15850208-15850227 None:intergenic 40.0%
TGAGGCCTTTATGTTATTCG+AGG - Chr2:15850407-15850426 MsG0280007465.01.T01:intron 40.0%
TTATTCGAGGGATGGAAGTA+AGG - Chr2:15850420-15850439 MsG0280007465.01.T01:intron 40.0%
TTCAATATGTTCAGGCCATG+GGG - Chr2:15850069-15850088 MsG0280007465.01.T01:CDS 40.0%
! CTGAACATATTGAAGGCGTA+CGG + Chr2:15850063-15850082 None:intergenic 40.0%
! TCTGAGGATTTTGTAATCGC+CGG + Chr2:15849947-15849966 None:intergenic 40.0%
!! CATCATCATCGATTTGGAGT+TGG + Chr2:15849633-15849652 None:intergenic 40.0%
!! CCTTCCCTGTTGATAATTGT+AGG - Chr2:15850590-15850609 MsG0280007465.01.T01:CDS 40.0%
AAGGATGCGTGAAGCAGATT+TGG + Chr2:15849774-15849793 None:intergenic 45.0%
ACCAGCTTCATGCCTCTTAT+TGG - Chr2:15849715-15849734 MsG0280007465.01.T01:intron 45.0%
CAGCTATCCAAGAGATTCTG+AGG + Chr2:15849963-15849982 None:intergenic 45.0%
CCATGGCCTGAACATATTGA+AGG + Chr2:15850070-15850089 None:intergenic 45.0%
CCTCTTCCTGCTGAAATAGA+AGG - Chr2:15850199-15850218 MsG0280007465.01.T01:CDS 45.0%
CCTTCAATATGTTCAGGCCA+TGG - Chr2:15850067-15850086 MsG0280007465.01.T01:CDS 45.0%
CCTTCTATTTCAGCAGGAAG+AGG + Chr2:15850202-15850221 None:intergenic 45.0%
CGTACGCCTTCAATATGTTC+AGG - Chr2:15850061-15850080 MsG0280007465.01.T01:CDS 45.0%
CGTGAAGCAGATTTGGCTAT+TGG + Chr2:15849767-15849786 None:intergenic 45.0%
GACTCCAGAATGTTAGGGAT+AGG + Chr2:15849868-15849887 None:intergenic 45.0%
GGAAGTAAGGTTCTGTTGGA+CGG - Chr2:15850433-15850452 MsG0280007465.01.T01:intron 45.0%
GGATAGGAAGTGGGAGAAAA+GGG + Chr2:15849852-15849871 None:intergenic 45.0%
GGATGGAAGTAAGGTTCTGT+TGG - Chr2:15850429-15850448 MsG0280007465.01.T01:intron 45.0%
GTCTTGAAAGGATGGTTGAG+TGG + Chr2:15849679-15849698 None:intergenic 45.0%
TCAATATGTTCAGGCCATGG+GGG - Chr2:15850070-15850089 MsG0280007465.01.T01:CDS 45.0%
TCTGAAAACATTGATCGCGC+CGG - Chr2:15849886-15849905 MsG0280007465.01.T01:CDS 45.0%
TGGTAAGCGGGACAAGATTT+CGG + Chr2:15849476-15849495 None:intergenic 45.0%
TTATCAACAGGGAAGGAAGG+TGG + Chr2:15850586-15850605 None:intergenic 45.0%
TTTGGCTATTGGAAATGGCG+AGG + Chr2:15849756-15849775 None:intergenic 45.0%
! ACCAATAAGAGGCATGAAGC+TGG + Chr2:15849719-15849738 None:intergenic 45.0%
! GTGATTTCATTAGGGGCGTA+AGG + Chr2:15849793-15849812 None:intergenic 45.0%
ATGAAGCTGGTACGCGTGAT+AGG + Chr2:15849706-15849725 None:intergenic 50.0%
ATTTCGGCGAGTATTTCCGG+CGG + Chr2:15849460-15849479 None:intergenic 50.0%
GGGATAGGAAGTGGGAGAAA+AGG + Chr2:15849853-15849872 None:intergenic 50.0%
GTGATAGGAGCGGTCTTGAA+AGG + Chr2:15849691-15849710 None:intergenic 50.0%
TAGGAGCGGTCTTGAAAGGA+TGG + Chr2:15849687-15849706 None:intergenic 50.0%
! ATTGAAGGCGTACGGCATAC+TGG + Chr2:15850055-15850074 None:intergenic 50.0%
! TTGAAGGCGTACGGCATACT+GGG + Chr2:15850054-15850073 None:intergenic 50.0%
!! GGACGGAACTCAGGTTTTGT+TGG - Chr2:15850450-15850469 MsG0280007465.01.T01:intron 50.0%
!!! CGGAACTCAGGTTTTGTTGG+AGG - Chr2:15850453-15850472 MsG0280007465.01.T01:intron 50.0%
GAAGCCGTGAACGCATACAC+CGG + Chr2:15849908-15849927 None:intergenic 55.0%
GAGCTGAAGAGTCTCACCTG+TGG + Chr2:15850006-15850025 None:intergenic 55.0%
GGCTATTGGAAATGGCGAGG+AGG + Chr2:15849753-15849772 None:intergenic 55.0%
GGTGTATGCGTTCACGGCTT+CGG - Chr2:15849907-15849926 MsG0280007465.01.T01:CDS 55.0%
GGTTCTGTTGGACGGAACTC+AGG - Chr2:15850441-15850460 MsG0280007465.01.T01:intron 55.0%
AGGGAGGACTTCTTGTCCCG+AGG - Chr2:15850997-15851016 MsG0280007465.01.T01:CDS 60.0%
CGAGTATTTCCGGCGGAAGG+CGG + Chr2:15849453-15849472 None:intergenic 60.0%
GCTGGTACGCGTGATAGGAG+CGG + Chr2:15849701-15849720 None:intergenic 60.0%
CGGCGAGTATTTCCGGCGGA+AGG + Chr2:15849456-15849475 None:intergenic 65.0%
! CGCGCCGGTGTATGCGTTCA+CGG - Chr2:15849901-15849920 MsG0280007465.01.T01:CDS 65.0%
! GGCGGCGGACCGCCTTCCGC+CGG - Chr2:15849441-15849460 MsG0280007465.01.T01:CDS 85.0%
Chromosome Type Strat End Strand Name
Chr2 gene 15849439 15851038 15849439 ID=MsG0280007465.01;Name=MsG0280007465.01
Chr2 mRNA 15849439 15851038 15849439 ID=MsG0280007465.01.T01;Parent=MsG0280007465.01;Name=MsG0280007465.01.T01;_AED=0.30;_eAED=0.30;_QI=0|0|0|1|0.5|0.66|3|0|364
Chr2 exon 15850549 15851038 15850549 ID=MsG0280007465.01.T01:exon:3466;Parent=MsG0280007465.01.T01
Chr2 exon 15849830 15850394 15849830 ID=MsG0280007465.01.T01:exon:3465;Parent=MsG0280007465.01.T01
Chr2 exon 15849439 15849478 15849439 ID=MsG0280007465.01.T01:exon:3464;Parent=MsG0280007465.01.T01
Chr2 CDS 15850549 15851038 15850549 ID=MsG0280007465.01.T01:cds;Parent=MsG0280007465.01.T01
Chr2 CDS 15849830 15850394 15849830 ID=MsG0280007465.01.T01:cds;Parent=MsG0280007465.01.T01
Chr2 CDS 15849439 15849478 15849439 ID=MsG0280007465.01.T01:cds;Parent=MsG0280007465.01.T01
Gene Sequence

>MsG0280007465.01.T01

ATGGCGGCGGACCGCCTTCCGCCGGAAATACTCGCCGAAATCTTGTCCCGCTTACCAGTTCAATCACTTCTCCGATTCCGATCAACTTCTAAATCACTTAAATCCATAATCGATTCTCATAACTTCATCAATCTTCACCTCAAAAACTCTATCAATAGATCCTTAATCATTCGACACAAATTCGATCTCTACCAACTCCAAATCGATGATGATGATGATTTCTCTAAGTCGATTATTCCACTCAACCATCCTTTCAAGACCGCTCCTATCACGCGTACCAGCTTCATGCCTCTTATTGGTTCATTTAACGGCCTCCTCGCCATTTCCAATAGCCAAATCTGCTTCACGCATCCTTACGCCCCTAATGAAATCACAATCTTGAACCCTAACACTCATAAACATCGGAATATCCCTTTTCTCCCACTTCCTATCCCTAACATTCTGGAGTCTGAAAACATTGATCGCGCCGGTGTATGCGTTCACGGCTTCGGCCATGGGGGTTTTGTTGAGAATTCTATTCACTGGGTAATGACTCGGACAATTGTTGAGTCACACCCTTGTTTCATTGTCGCGTTTAACCTAACACTTGAGACTTTCAATGTGGTTCCTCTTCCTGCTGAAATAGAAGGTGAAGAGATTAATAGTAATAATGAGAGTTCTACAATTGCTGTTGCTGTTTTAGGAGAGTGTCTTTGTATGACTGTGAATTATGAAACTACTAAAATTGATGTGTGGGTGATGAAAGAATATGGTTCTAGAGATAGTTGGTGTAAACTTTTTACTTTGGATAAATCGCGGTTCACTTTGAAATCTTTGAGGCCTTTATGTTATTCGAGGGATGGAAGTAAGGTTCTGTTGGACGGAACTCAGGTTTTGTTGGAGGTAAATCATAGGAAGCTGTTTTGGTATGATTTGAAGAGTGAACAAATTAGTTATGTTGAAGGAATTCCTAATTTGGATGAAGCTATGATTTGTGTTGAAAGTCTTGTACCACCTTCCTTCCCTGTTGATAATTGTAGGAAGAAGGAGAATCATACAAACAAGAGAAAGAAACGGGAGGACTTCTTGTCCCGAGGATTTAAATTGAGATTGTAA
Protein sequence

>MsG0280007465.01.T01

MAADRLPPEILAEILSRLPVQSLLRFRSTSKSLKSIIDSHNFINLHLKNSINRSLIIRHKFDLYQLQIDDDDDFSKSIIPLNHPFKTAPITRTSFMPLIGSFNGLLAISNSQICFTHPYAPNEITILNPNTHKHRNIPFLPLPIPNILESENIDRAGVCVHGFGHGGFVENSIHWVMTRTIVESHPCFIVAFNLTLETFNVVPLPAEIEGEEINSNNESSTIAVAVLGECLCMTVNYETTKIDVWVMKEYGSRDSWCKLFTLDKSRFTLKSLRPLCYSRDGSKVLLDGTQVLLEVNHRKLFWYDLKSEQISYVEGIPNLDEAMICVESLVPPSFPVDNCRKKENHTNKRKKREDFLSRGFKLRL*