AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009535.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009535.01.T01 MTR_2g065840 71.026 390 72 6 1 350 1 389 0.0 527
MsG0280009535.01.T01 MTR_2g065860 72.051 390 69 6 1 351 1 389 0.0 526
MsG0280009535.01.T01 MTR_2g066150 56.911 369 133 8 1 351 1 361 5.59e-132 381
MsG0280009535.01.T01 MTR_2g066200 51.399 393 145 8 1 351 1 389 1.46e-119 350
MsG0280009535.01.T01 MTR_2g066170 52.685 391 141 10 1 351 1 387 6.14e-117 344
MsG0280009535.01.T01 MTR_8g039500 53.541 353 123 9 1 319 1 346 3.43e-111 328
MsG0280009535.01.T01 MTR_1g050308 45.330 364 148 10 1 351 23 348 4.56e-83 256
MsG0280009535.01.T01 MTR_8g063440 39.186 393 188 9 1 351 1 384 7.01e-80 249
MsG0280009535.01.T01 MTR_8g464770 39.948 383 182 9 1 344 1 374 7.25e-80 249
MsG0280009535.01.T01 MTR_3g011120 44.324 370 162 11 6 339 8 369 7.75e-78 243
MsG0280009535.01.T01 MTR_0129s0070 40.796 402 175 14 2 351 7 397 1.42e-75 239
MsG0280009535.01.T01 MTR_8g467670 41.775 383 175 12 7 351 8 380 6.74e-74 234
MsG0280009535.01.T01 MTR_8g064790 41.714 350 182 9 5 351 8 338 1.14e-73 231
MsG0280009535.01.T01 MTR_5g097330 40.347 404 174 14 1 346 5 399 1.63e-73 233
MsG0280009535.01.T01 MTR_0129s0120 40.506 395 174 13 6 349 11 395 3.21e-73 232
MsG0280009535.01.T01 MTR_8g064890 39.796 392 179 11 6 351 9 389 4.35e-73 232
MsG0280009535.01.T01 MTR_5g096910 38.402 388 183 10 9 349 14 392 3.11e-72 229
MsG0280009535.01.T01 MTR_2g034700 41.600 375 170 13 6 343 7 369 3.90e-72 229
MsG0280009535.01.T01 MTR_8g012090 41.765 340 167 9 1 319 5 334 7.28e-72 227
MsG0280009535.01.T01 MTR_5g097350 40.431 371 164 13 21 346 25 383 4.03e-71 226
MsG0280009535.01.T01 MTR_8g036970 44.242 330 158 10 16 337 2 313 1.14e-69 223
MsG0280009535.01.T01 MTR_4g102410 38.366 404 188 12 1 348 50 448 1.48e-69 224
MsG0280009535.01.T01 MTR_5g037680 37.047 386 189 13 11 349 7 385 4.29e-69 223
MsG0280009535.01.T01 MTR_1g007450 39.744 390 173 12 1 336 1 382 6.76e-69 222
MsG0280009535.01.T01 MTR_6g079810 38.406 414 177 15 2 348 59 461 2.44e-68 222
MsG0280009535.01.T01 MTR_1g050312 46.207 290 104 8 73 331 8 276 4.98e-68 215
MsG0280009535.01.T01 MTR_5g096630 35.922 412 198 13 1 350 7 414 5.83e-67 217
MsG0280009535.01.T01 MTR_4g117550 37.150 393 171 12 6 351 11 374 1.54e-66 214
MsG0280009535.01.T01 MTR_0134s0060 35.782 422 191 15 1 351 1 413 7.59e-66 214
MsG0280009535.01.T01 MTR_6g080680 38.918 388 179 15 5 344 12 389 8.45e-66 213
MsG0280009535.01.T01 MTR_0054s0100 35.854 410 196 13 2 351 7 409 3.83e-65 212
MsG0280009535.01.T01 MTR_0054s0090 35.610 410 195 12 2 351 7 407 5.97e-64 209
MsG0280009535.01.T01 MTR_5g097220 37.621 412 178 15 7 350 8 408 7.22e-64 208
MsG0280009535.01.T01 MTR_4g028510 38.272 405 189 14 1 350 4 402 1.23e-63 209
MsG0280009535.01.T01 MTR_7g069550 36.776 397 178 15 6 342 8 391 1.82e-63 207
MsG0280009535.01.T01 MTR_6g086825 36.585 410 187 11 5 351 62 461 1.99e-63 209
MsG0280009535.01.T01 MTR_2g021800 35.800 419 183 13 7 351 11 417 6.23e-63 206
MsG0280009535.01.T01 MTR_4g028660 37.750 400 187 14 1 346 4 395 6.45e-63 206
MsG0280009535.01.T01 MTR_4g027450 37.750 400 187 14 1 346 4 395 6.45e-63 206
MsG0280009535.01.T01 MTR_5g024260 39.785 372 166 12 9 328 19 384 7.20e-63 206
MsG0280009535.01.T01 MTR_2g022000 35.891 404 174 12 7 337 11 402 7.39e-63 207
MsG0280009535.01.T01 MTR_6g090180 36.049 405 192 13 2 346 37 434 1.40e-62 206
MsG0280009535.01.T01 MTR_6g079770 36.145 415 189 13 3 348 8 415 3.59e-62 204
MsG0280009535.01.T01 MTR_0831s0010 37.065 402 182 12 2 350 38 421 5.34e-62 204
MsG0280009535.01.T01 MTR_4g130330 37.562 402 177 15 9 350 4 391 5.77e-61 201
MsG0280009535.01.T01 MTR_2g021950 34.430 395 204 11 1 346 5 393 1.22e-60 199
MsG0280009535.01.T01 MTR_8g046310 37.203 379 195 12 2 345 3 373 1.75e-60 199
MsG0280009535.01.T01 MTR_4g028680 37.717 403 194 11 1 351 4 401 1.77e-60 199
MsG0280009535.01.T01 MTR_4g014370 33.412 422 195 14 3 351 2 410 9.81e-59 195
MsG0280009535.01.T01 MTR_7g017130 34.975 406 195 12 1 351 7 398 1.07e-58 195
MsG0280009535.01.T01 MTR_1g053510 34.889 407 193 12 2 345 4 401 1.70e-58 194
MsG0280009535.01.T01 MTR_2g038680 35.765 425 178 15 1 349 5 410 1.72e-58 194
MsG0280009535.01.T01 MTR_8g063330 39.444 360 151 13 1 305 1 348 2.40e-58 196
MsG0280009535.01.T01 MTR_4g014370 35.695 367 177 13 3 351 13 338 1.38e-57 190
MsG0280009535.01.T01 MTR_5g059430 34.568 405 193 15 11 348 25 424 3.79e-57 191
MsG0280009535.01.T01 MTR_6g461660 36.456 395 196 12 1 346 8 396 6.08e-57 190
MsG0280009535.01.T01 MTR_8g033140 34.058 414 194 14 8 351 15 419 1.21e-56 190
MsG0280009535.01.T01 MTR_2g021770 34.217 415 202 14 1 351 1 408 1.28e-56 189
MsG0280009535.01.T01 MTR_7g111700 36.828 372 185 10 11 351 14 366 8.70e-56 186
MsG0280009535.01.T01 MTR_7g017170 33.971 418 195 13 1 351 7 410 1.01e-55 187
MsG0280009535.01.T01 MTR_6g478230 35.025 394 196 11 9 351 24 408 2.37e-55 187
MsG0280009535.01.T01 MTR_5g072060 38.440 359 161 15 11 325 5 347 5.01e-55 190
MsG0280009535.01.T01 MTR_5g069390 35.013 397 193 15 11 348 25 415 6.66e-55 187
MsG0280009535.01.T01 MTR_7g017040 32.663 398 208 10 1 346 5 394 1.22e-54 184
MsG0280009535.01.T01 MTR_4g055820 36.538 364 162 10 2 305 4 358 1.63e-54 183
MsG0280009535.01.T01 MTR_7g079640 36.267 375 190 15 3 341 4 365 1.51e-53 181
MsG0280009535.01.T01 MTR_2g104340 35.122 410 182 16 6 351 8 397 3.39e-53 180
MsG0280009535.01.T01 MTR_4g007940 37.931 348 153 14 60 351 2 342 6.21e-53 178
MsG0280009535.01.T01 MTR_4g028750 36.316 380 183 12 1 328 4 376 1.33e-52 179
MsG0280009535.01.T01 MTR_5g097680 33.100 429 201 14 1 350 6 427 2.84e-52 179
MsG0280009535.01.T01 MTR_1g007410 33.835 399 196 13 1 342 11 398 3.72e-52 178
MsG0280009535.01.T01 MTR_2g104280 35.523 411 184 17 7 350 9 405 2.48e-51 176
MsG0280009535.01.T01 MTR_8g041270 34.606 419 194 15 1 351 1 407 2.59e-51 176
MsG0280009535.01.T01 MTR_2g064950 35.385 390 185 17 6 341 4 380 3.72e-51 175
MsG0280009535.01.T01 MTR_1g098180 33.784 370 182 11 11 344 18 360 4.10e-51 174
MsG0280009535.01.T01 MTR_8g063370 37.027 370 179 16 14 341 26 383 9.85e-51 174
MsG0280009535.01.T01 MTR_8g046220 33.492 421 183 13 2 346 3 402 3.89e-49 170
MsG0280009535.01.T01 MTR_8g013000 35.556 360 163 13 6 307 4 352 2.31e-48 168
MsG0280009535.01.T01 MTR_5g091140 35.864 382 192 16 1 340 37 407 4.21e-48 167
MsG0280009535.01.T01 MTR_8g033110 32.075 424 205 13 1 350 1 415 6.89e-48 167
MsG0280009535.01.T01 MTR_8g018110 31.553 412 205 11 1 350 1 397 1.74e-47 165
MsG0280009535.01.T01 MTR_8g033090 33.176 425 193 17 2 350 3 412 7.84e-47 164
MsG0280009535.01.T01 MTR_8g063310 30.077 389 209 13 3 341 13 388 2.38e-46 162
MsG0280009535.01.T01 MTR_5g043605 34.492 374 149 13 8 345 21 334 4.01e-46 161
MsG0280009535.01.T01 MTR_6g078010 35.398 339 168 13 49 345 1 330 3.24e-45 158
MsG0280009535.01.T01 MTR_1g007430 35.417 336 147 10 23 307 7 323 1.47e-44 156
MsG0280009535.01.T01 MTR_8g046200 31.925 426 199 13 2 346 3 418 2.82e-44 157
MsG0280009535.01.T01 MTR_7g104710 38.380 284 147 9 3 263 8 286 6.93e-44 154
MsG0280009535.01.T01 MTR_5g097850 39.431 246 118 10 116 349 18 244 8.90e-42 146
MsG0280009535.01.T01 MTR_6g090250 30.923 401 182 13 30 345 29 419 1.72e-41 150
MsG0280009535.01.T01 MTR_5g092850 33.663 404 180 16 11 346 15 398 1.80e-41 150
MsG0280009535.01.T01 MTR_5g074800 33.618 351 159 15 51 342 1 336 1.11e-40 146
MsG0280009535.01.T01 MTR_5g023970 44.792 192 98 5 165 351 9 197 4.10e-40 140
MsG0280009535.01.T01 MTR_7g023800 31.975 319 176 12 51 343 1 304 5.81e-40 143
MsG0280009535.01.T01 MTR_5g092860 34.670 349 159 16 30 327 20 350 3.84e-39 142
MsG0280009535.01.T01 MTR_5g068490 37.500 288 138 13 89 351 8 278 4.40e-38 137
MsG0280009535.01.T01 MTR_2g021920 44.865 185 89 6 171 346 89 269 1.30e-37 136
MsG0280009535.01.T01 MTR_7g016930 33.439 314 154 11 5 281 9 304 3.86e-37 136
MsG0280009535.01.T01 MTR_5g074850 31.043 393 191 16 1 327 23 401 3.42e-36 139
MsG0280009535.01.T01 MTR_5g096900 33.439 314 157 12 1 267 1 309 6.55e-36 133
MsG0280009535.01.T01 MTR_5g086590 38.034 234 102 10 133 332 14 238 1.90e-34 127
MsG0280009535.01.T01 MTR_6g090260 39.286 196 99 6 163 345 71 259 6.46e-34 126
MsG0280009535.01.T01 MTR_6g090260 61.017 59 22 1 1 58 8 66 2.21e-13 69.7
MsG0280009535.01.T01 MTR_8g063350 30.811 370 170 14 11 340 13 336 1.08e-33 127
MsG0280009535.01.T01 MTR_5g075370 30.829 386 187 15 9 327 5 377 1.44e-33 132
MsG0280009535.01.T01 MTR_0129s0050 40.860 186 100 5 171 350 227 408 1.63e-33 128
MsG0280009535.01.T01 MTR_0129s0050 47.973 148 63 4 6 148 8 146 8.51e-31 121
MsG0280009535.01.T01 MTR_8g073570 46.405 153 69 6 1 149 1 144 1.87e-32 119
MsG0280009535.01.T01 MTR_5g037190 34.661 251 129 7 1 221 5 250 6.01e-32 120
MsG0280009535.01.T01 MTR_6g087620 31.884 276 151 9 80 328 1 266 6.07e-32 121
MsG0280009535.01.T01 MTR_5g022850 28.040 403 185 13 1 351 5 354 2.20e-31 121
MsG0280009535.01.T01 MTR_5g064700 29.118 340 166 11 80 350 35 368 3.93e-31 121
MsG0280009535.01.T01 MTR_8g032980 42.675 157 77 4 1 150 1 151 6.34e-29 116
MsG0280009535.01.T01 MTR_8g032980 35.000 200 101 8 171 351 232 421 5.05e-18 85.1
MsG0280009535.01.T01 MTR_3g047580 40.541 148 65 3 1 146 1 127 1.00e-27 105
MsG0280009535.01.T01 MTR_6g079790 29.503 322 152 9 92 351 2 310 1.19e-27 110
MsG0280009535.01.T01 MTR_5g097820 30.337 267 133 9 1 219 8 269 5.91e-27 107
MsG0280009535.01.T01 MTR_6g465360 40.667 150 81 5 1 146 51 196 1.15e-26 107
MsG0280009535.01.T01 MTR_1g076810 34.711 242 107 9 2 200 8 241 1.75e-26 110
MsG0280009535.01.T01 MTR_6g070880 40.000 155 86 4 7 157 27 178 2.12e-26 106
MsG0280009535.01.T01 MTR_6g071420 40.000 155 86 4 7 157 27 178 2.12e-26 106
MsG0280009535.01.T01 MTR_6g004450 38.776 196 96 7 165 349 86 268 3.00e-26 105
MsG0280009535.01.T01 MTR_5g076490 34.194 310 161 11 80 350 31 336 5.75e-26 106
MsG0280009535.01.T01 MTR_7g019250 49.242 132 60 3 212 338 295 424 6.29e-26 108
MsG0280009535.01.T01 MTR_1g050305 65.060 83 26 1 249 328 21 103 1.20e-25 100
MsG0280009535.01.T01 MTR_6g079730 43.382 136 68 5 215 345 14 145 2.13e-25 100
MsG0280009535.01.T01 MTR_5g096920 38.816 152 89 3 199 348 1 150 6.96e-25 99.0
MsG0280009535.01.T01 MTR_8g033000 37.824 193 98 6 171 350 180 363 6.35e-24 101
MsG0280009535.01.T01 MTR_8g033000 37.963 108 57 4 49 152 1 102 7.70e-12 66.2
MsG0280009535.01.T01 MTR_5g097660 41.525 118 66 2 230 346 1 116 2.33e-23 94.0
MsG0280009535.01.T01 MTR_4g016000 37.952 166 74 6 144 300 45 190 7.08e-23 95.5
MsG0280009535.01.T01 MTR_5g097540 29.818 275 134 9 49 307 1 232 8.54e-23 97.1
MsG0280009535.01.T01 MTR_2g022020 40.000 120 65 3 230 346 1 116 4.00e-22 90.5
MsG0280009535.01.T01 MTR_5g094350 31.203 266 142 9 8 233 9 273 7.22e-22 94.0
MsG0280009535.01.T01 MTR_5g066420 37.086 151 83 3 199 339 1 149 9.59e-22 90.5
MsG0280009535.01.T01 MTR_5g097650 36.765 136 76 3 11 146 21 146 1.63e-21 90.1
MsG0280009535.01.T01 MTR_8g035690 30.408 319 185 13 51 349 1 302 1.77e-21 93.6
MsG0280009535.01.T01 MTR_3g018750 35.079 191 104 6 171 346 52 237 4.03e-21 91.3
MsG0280009535.01.T01 MTR_3g030620 34.356 163 93 5 197 348 21 180 7.06e-20 86.3
MsG0280009535.01.T01 MTR_5g096070 38.750 160 58 7 6 159 20 145 8.81e-20 85.5
MsG0280009535.01.T01 MTR_5g036250 46.154 104 53 2 247 350 11 111 1.62e-19 85.5
MsG0280009535.01.T01 MTR_7g017150 35.106 188 110 5 171 351 86 268 1.01e-18 85.1
MsG0280009535.01.T01 MTR_7g017120 34.574 188 111 5 171 351 85 267 1.76e-18 84.3
MsG0280009535.01.T01 MTR_6g009350 38.816 152 77 5 171 313 51 195 1.07e-17 80.5
MsG0280009535.01.T01 MTR_3g019290 42.735 117 57 4 1 109 1 115 2.25e-17 79.3
MsG0280009535.01.T01 MTR_8g041440 41.000 100 45 1 5 104 8 93 2.90e-15 71.6
MsG0280009535.01.T01 MTR_1g115205 30.672 238 106 12 2 205 23 235 2.48e-14 72.4
MsG0280009535.01.T01 MTR_7g101200 32.850 207 102 10 127 307 33 228 2.51e-14 72.0
MsG0280009535.01.T01 MTR_2g104260 30.249 281 126 11 6 227 8 277 3.71e-14 72.4
MsG0280009535.01.T01 MTR_5g086780 41.489 94 53 1 233 326 137 228 6.33e-14 70.9
MsG0280009535.01.T01 MTR_5g086780 65.306 49 17 0 5 53 9 57 3.63e-12 65.9
MsG0280009535.01.T01 MTR_4g030830 27.885 312 183 12 12 285 9 316 2.77e-13 70.9
MsG0280009535.01.T01 MTR_6g087660 58.621 58 23 1 2 58 7 64 2.54e-12 65.5
MsG0280009535.01.T01 MTR_5g098950 33.566 143 68 3 165 307 158 273 3.96e-12 66.2
MsG0280009535.01.T01 MTR_1g097560 24.432 352 200 17 12 327 36 357 4.17e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 85 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGGAGTTGTATTTGATTTC+TGG 0.134759 2:-53905064 MsG0280009535.01.T01:CDS
GAAAACATGTTCATGAAATA+AGG 0.144899 2:+53905160 None:intergenic
TTGACAAAAGCAATTAATTT+TGG 0.148055 2:+53904150 None:intergenic
TCTGATATTAAGGGGTTTAT+TGG 0.189541 2:-53904332 MsG0280009535.01.T01:CDS
TTCGGTGTTCGGTAGGTATT+GGG 0.209576 2:-53904631 MsG0280009535.01.T01:CDS
ATTCAGAACATTGGTGTTAA+AGG 0.211274 2:-53904233 MsG0280009535.01.T01:CDS
AGATATTTGGCGGAGTTAAA+TGG 0.213428 2:-53904473 MsG0280009535.01.T01:CDS
TGGACCCTTATCTGATATTA+AGG 0.214953 2:-53904342 MsG0280009535.01.T01:CDS
ACAATGGGTTGATAGATATT+TGG 0.226201 2:-53904486 MsG0280009535.01.T01:CDS
ATTGCATGTTCATCTGTTAA+TGG 0.232304 2:-53904962 MsG0280009535.01.T01:CDS
ATCGTACAATGAACTATATT+TGG 0.247843 2:+53905123 None:intergenic
CTTCGGTGTTCGGTAGGTAT+TGG 0.259726 2:-53904632 MsG0280009535.01.T01:CDS
TTATATCGTTCTCGAATAAA+AGG 0.263133 2:+53905001 None:intergenic
ATTGGGAATATATAGTTTAA+AGG 0.266682 2:-53904698 MsG0280009535.01.T01:intron
TTTCCCTACTCTCATGGTTT+AGG 0.273859 2:+53905086 None:intergenic
TATAGTTTAAAGGTAACTAT+TGG 0.276390 2:-53904688 MsG0280009535.01.T01:intron
ACAGATGAACATGCAATAAA+AGG 0.283974 2:+53904968 None:intergenic
AGAATTACCTATACTATTCA+TGG 0.313949 2:-53904810 MsG0280009535.01.T01:CDS
AAAACATGTTCATGAAATAA+GGG 0.358662 2:+53905161 None:intergenic
ACCTATACTATTCATGGATT+CGG 0.365271 2:-53904804 MsG0280009535.01.T01:intron
AAGAAGTGGTCTTTACATAC+TGG 0.374720 2:+53904432 None:intergenic
ATTAAGGGGTTTATTGGAGC+AGG 0.378072 2:-53904326 MsG0280009535.01.T01:CDS
ATAGTTTAAAGGTAACTATT+GGG 0.409315 2:-53904687 MsG0280009535.01.T01:intron
GGTATTGGGGTTCACTTGGA+TGG 0.411330 2:-53904617 MsG0280009535.01.T01:CDS
GGACCCTTATCTGATATTAA+GGG 0.414023 2:-53904341 MsG0280009535.01.T01:CDS
CCACATATCAATATCAATGT+TGG 0.421666 2:-53904408 MsG0280009535.01.T01:CDS
AAACCCCTTAATATCAGATA+AGG 0.433730 2:+53904337 None:intergenic
CACATATCAATATCAATGTT+GGG 0.437372 2:-53904407 MsG0280009535.01.T01:CDS
CCCTACTCTCATGGTTTAGG+AGG 0.444078 2:+53905089 None:intergenic
GATATTTGGCGGAGTTAAAT+GGG 0.444773 2:-53904472 MsG0280009535.01.T01:CDS
ACTCCCTTTCCCTACTCTCA+TGG 0.449682 2:+53905080 None:intergenic
GAGGGTAGGAAAGTGGTAAC+AGG 0.455708 2:+53904524 None:intergenic
AGAACAAAGTGCCATTGAAC+TGG 0.455826 2:-53905030 MsG0280009535.01.T01:CDS
ATATCAATGTTGGGTGAGCC+CGG 0.470256 2:-53904398 MsG0280009535.01.T01:CDS
AGAGCGTACTCTTCGGTGTT+CGG 0.474535 2:-53904642 MsG0280009535.01.T01:CDS
GGTAGGTATTGGGGTTCACT+TGG 0.475087 2:-53904621 MsG0280009535.01.T01:CDS
ATAGAGAAGACGATATCTTC+AGG 0.477014 2:+53905259 None:intergenic
AAAGGACGTGGCCAGTTCAA+TGG 0.481331 2:+53905019 None:intergenic
TGGATTAAGCTCTTCGATGT+TGG 0.483726 2:-53904362 MsG0280009535.01.T01:CDS
GTCATTCAACTGTTGTATTG+TGG 0.493633 2:-53904910 MsG0280009535.01.T01:CDS
AATCCATGATTCATTGATCC+CGG 0.494814 2:+53904379 None:intergenic
AACCCCTTAATATCAGATAA+GGG 0.499543 2:+53904338 None:intergenic
TTACTTGTGTAAACAAACAT+TGG 0.508427 2:-53905201 MsG0280009535.01.T01:CDS
ACCGAATCCATGAATAGTAT+AGG 0.518449 2:+53904803 None:intergenic
ATATGCGAGAGCGTACTCTT+CGG 0.520264 2:-53904649 MsG0280009535.01.T01:CDS
TACCTTTGAAATCTGTGAAG+CGG 0.522288 2:-53905225 MsG0280009535.01.T01:CDS
GATGGAGTGTGCCATTGGTC+GGG 0.522289 2:-53904599 MsG0280009535.01.T01:CDS
ATCCATGATTCATTGATCCC+GGG 0.525616 2:+53904380 None:intergenic
CCTCCTAAACCATGAGAGTA+GGG 0.525846 2:-53905089 MsG0280009535.01.T01:CDS
AGCCCGGGATCAATGAATCA+TGG 0.526045 2:-53904382 MsG0280009535.01.T01:CDS
CATGGCTGCGATCAATGAAA+AGG 0.527321 2:-53905296 MsG0280009535.01.T01:CDS
GGATGGAGTGTGCCATTGGT+CGG 0.534223 2:-53904600 MsG0280009535.01.T01:CDS
ACTTGGATGGAGTGTGCCAT+TGG 0.536817 2:-53904604 MsG0280009535.01.T01:CDS
AGACTTATATGATATACAAT+GGG 0.539898 2:-53904501 MsG0280009535.01.T01:CDS
ATGGGTTGATAGATATTTGG+CGG 0.544177 2:-53904483 MsG0280009535.01.T01:CDS
TTAAGGGGTTTATTGGAGCA+GGG 0.546954 2:-53904325 MsG0280009535.01.T01:CDS
ATATGTAATTCATCGGTAGC+GGG 0.552598 2:+53904884 None:intergenic
GACCCTTATCTGATATTAAG+GGG 0.556028 2:-53904340 MsG0280009535.01.T01:CDS
TATCAATGTTGGGTGAGCCC+GGG 0.558986 2:-53904397 MsG0280009535.01.T01:CDS
AAACCATGAGAGTAGGGAAA+GGG 0.560122 2:-53905083 MsG0280009535.01.T01:CDS
CAGACTTATATGATATACAA+TGG 0.560609 2:-53904502 MsG0280009535.01.T01:CDS
AGCAACAATATGTAATTCAT+CGG 0.562881 2:+53904877 None:intergenic
AACAGATTGTGAAACATATG+TGG 0.564451 2:-53904573 MsG0280009535.01.T01:CDS
GTTTATTGGAGCAGGGAGGA+AGG 0.564455 2:-53904318 MsG0280009535.01.T01:CDS
CGTACTCTTCGGTGTTCGGT+AGG 0.565634 2:-53904638 MsG0280009535.01.T01:CDS
AATATGTAATTCATCGGTAG+CGG 0.566481 2:+53904883 None:intergenic
AGAAGTGGTCTTTACATACT+GGG 0.573635 2:+53904433 None:intergenic
CCAACATTGATATTGATATG+TGG 0.578112 2:+53904408 None:intergenic
TGAATTACATATTGTTGCTG+AGG 0.581979 2:-53904873 MsG0280009535.01.T01:CDS
TAAGTCTGAGGGTAGGAAAG+TGG 0.583122 2:+53904517 None:intergenic
TCCTCCTAAACCATGAGAGT+AGG 0.584534 2:-53905090 MsG0280009535.01.T01:CDS
AACCGCTTCACAGATTTCAA+AGG 0.593493 2:+53905223 None:intergenic
CGTTCTCGAATAAAAGGACG+TGG 0.600248 2:+53905007 None:intergenic
TTGTATATCATATAAGTCTG+AGG 0.600411 2:+53904505 None:intergenic
ACCAATGTTCTGAATTACTC+CGG 0.603398 2:+53904241 None:intergenic
TGTATATCATATAAGTCTGA+GGG 0.604898 2:+53904506 None:intergenic
AGGGGTTTATTGGAGCAGGG+AGG 0.607061 2:-53904322 MsG0280009535.01.T01:CDS
TCATGGTTTAGGAGGAAACA+TGG 0.627836 2:+53905097 None:intergenic
TCGGTGTTCGGTAGGTATTG+GGG 0.630313 2:-53904630 MsG0280009535.01.T01:CDS
ACCGGAGTAATTCAGAACAT+TGG 0.630709 2:-53904242 MsG0280009535.01.T01:CDS
TAAACCATGAGAGTAGGGAA+AGG 0.631670 2:-53905084 MsG0280009535.01.T01:CDS
TATCATATAAGTCTGAGGGT+AGG 0.650553 2:+53904510 None:intergenic
CGGGAATAACACAATGATCG+TGG 0.668548 2:+53904849 None:intergenic
TATTGATATGTGGAAAGAAG+TGG 0.676003 2:+53904418 None:intergenic
GAACATGCAATAAAAGGACA+AGG 0.678669 2:+53904974 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGCTTTTTTTAAAAATATAT+CGG + Chr2:53905166-53905185 None:intergenic 10.0%
!! TTCTCTATCATTATAAAAAT+CGG + Chr2:53904632-53904651 None:intergenic 15.0%
!! AAAACATGTTCATGAAATAA+GGG + Chr2:53904300-53904319 None:intergenic 20.0%
!! AGACTTATATGATATACAAT+GGG - Chr2:53904957-53904976 MsG0280009535.01.T01:CDS 20.0%
!! ATAGTTTAAAGGTAACTATT+GGG - Chr2:53904771-53904790 MsG0280009535.01.T01:intron 20.0%
!! ATTGGGAATATATAGTTTAA+AGG - Chr2:53904760-53904779 MsG0280009535.01.T01:intron 20.0%
!! TAAACTATATATTCCCAATA+GGG + Chr2:53904759-53904778 None:intergenic 20.0%
!! TATAGTTTAAAGGTAACTAT+TGG - Chr2:53904770-53904789 MsG0280009535.01.T01:intron 20.0%
!! TCTCTATCATTATAAAAATC+GGG + Chr2:53904631-53904650 None:intergenic 20.0%
!! TTAAACTATATATTCCCAAT+AGG + Chr2:53904760-53904779 None:intergenic 20.0%
!!! ATATTTGGAAACCATATTTT+TGG + Chr2:53904323-53904342 None:intergenic 20.0%
! AAACTATATATTCCCAATAG+GGG + Chr2:53904758-53904777 None:intergenic 25.0%
! AGAATTACCTATACTATTCA+TGG - Chr2:53904648-53904667 MsG0280009535.01.T01:CDS 25.0%
! AGCAACAATATGTAATTCAT+CGG + Chr2:53904584-53904603 None:intergenic 25.0%
! ATCGTACAATGAACTATATT+TGG + Chr2:53904338-53904357 None:intergenic 25.0%
! CACATATCAATATCAATGTT+GGG - Chr2:53905051-53905070 MsG0280009535.01.T01:CDS 25.0%
! CAGACTTATATGATATACAA+TGG - Chr2:53904956-53904975 MsG0280009535.01.T01:CDS 25.0%
! GAAAACATGTTCATGAAATA+AGG + Chr2:53904301-53904320 None:intergenic 25.0%
! TGTATATCATATAAGTCTGA+GGG + Chr2:53904955-53904974 None:intergenic 25.0%
! TTACTTGTGTAAACAAACAT+TGG - Chr2:53904257-53904276 MsG0280009535.01.T01:CDS 25.0%
! TTATATCGTTCTCGAATAAA+AGG + Chr2:53904460-53904479 None:intergenic 25.0%
! TTGTATATCATATAAGTCTG+AGG + Chr2:53904956-53904975 None:intergenic 25.0%
!! GAACATGTTTTCCAAAAATA+TGG - Chr2:53904309-53904328 MsG0280009535.01.T01:CDS 25.0%
!!! GCATATTTTGATTTAACTAC+CGG - Chr2:53905198-53905217 MsG0280009535.01.T01:CDS 25.0%
AAAAACATTCTTCCAATCGT+AGG - Chr2:53905282-53905301 MsG0280009535.01.T01:CDS 30.0%
AAACCCCTTAATATCAGATA+AGG + Chr2:53905124-53905143 None:intergenic 30.0%
AACAGATTGTGAAACATATG+TGG - Chr2:53904885-53904904 MsG0280009535.01.T01:CDS 30.0%
AACCCCTTAATATCAGATAA+GGG + Chr2:53905123-53905142 None:intergenic 30.0%
AATATGTAATTCATCGGTAG+CGG + Chr2:53904578-53904597 None:intergenic 30.0%
ACAGATGAACATGCAATAAA+AGG + Chr2:53904493-53904512 None:intergenic 30.0%
ACCTATACTATTCATGGATT+CGG - Chr2:53904654-53904673 MsG0280009535.01.T01:CDS 30.0%
ATTGCATGTTCATCTGTTAA+TGG - Chr2:53904496-53904515 MsG0280009535.01.T01:CDS 30.0%
CCAACATTGATATTGATATG+TGG + Chr2:53905053-53905072 None:intergenic 30.0%
CCACATATCAATATCAATGT+TGG - Chr2:53905050-53905069 MsG0280009535.01.T01:CDS 30.0%
TATTGATATGTGGAAAGAAG+TGG + Chr2:53905043-53905062 None:intergenic 30.0%
TCTGATATTAAGGGGTTTAT+TGG - Chr2:53905126-53905145 MsG0280009535.01.T01:CDS 30.0%
TGAATTACATATTGTTGCTG+AGG - Chr2:53904585-53904604 MsG0280009535.01.T01:CDS 30.0%
! CTTCGATATGTTGATTATCA+TGG - Chr2:53904708-53904727 MsG0280009535.01.T01:intron 30.0%
! TAATTTTGGATTCCTACGAT+TGG + Chr2:53905297-53905316 None:intergenic 30.0%
!! ACAATGGGTTGATAGATATT+TGG - Chr2:53904972-53904991 MsG0280009535.01.T01:CDS 30.0%
!! ATTCAGAACATTGGTGTTAA+AGG - Chr2:53905225-53905244 MsG0280009535.01.T01:CDS 30.0%
AAGAAGTGGTCTTTACATAC+TGG + Chr2:53905029-53905048 None:intergenic 35.0%
AATCCATGATTCATTGATCC+CGG + Chr2:53905082-53905101 None:intergenic 35.0%
ACCGAATCCATGAATAGTAT+AGG + Chr2:53904658-53904677 None:intergenic 35.0%
AGAAGTGGTCTTTACATACT+GGG + Chr2:53905028-53905047 None:intergenic 35.0%
AGATATTTGGCGGAGTTAAA+TGG - Chr2:53904985-53905004 MsG0280009535.01.T01:CDS 35.0%
ATAGAGAAGACGATATCTTC+AGG + Chr2:53904202-53904221 None:intergenic 35.0%
ATATGTAATTCATCGGTAGC+GGG + Chr2:53904577-53904596 None:intergenic 35.0%
GAACATGCAATAAAAGGACA+AGG + Chr2:53904487-53904506 None:intergenic 35.0%
GACCCTTATCTGATATTAAG+GGG - Chr2:53905118-53905137 MsG0280009535.01.T01:CDS 35.0%
GATATTTGGCGGAGTTAAAT+GGG - Chr2:53904986-53905005 MsG0280009535.01.T01:CDS 35.0%
GGACCCTTATCTGATATTAA+GGG - Chr2:53905117-53905136 MsG0280009535.01.T01:CDS 35.0%
GTCATTCAACTGTTGTATTG+TGG - Chr2:53904548-53904567 MsG0280009535.01.T01:CDS 35.0%
TATCATATAAGTCTGAGGGT+AGG + Chr2:53904951-53904970 None:intergenic 35.0%
TGGACCCTTATCTGATATTA+AGG - Chr2:53905116-53905135 MsG0280009535.01.T01:CDS 35.0%
! ACCAATGTTCTGAATTACTC+CGG + Chr2:53905220-53905239 None:intergenic 35.0%
! AGGGAGTTGTATTTGATTTC+TGG - Chr2:53904394-53904413 MsG0280009535.01.T01:CDS 35.0%
!! ATGGGTTGATAGATATTTGG+CGG - Chr2:53904975-53904994 MsG0280009535.01.T01:CDS 35.0%
!! TACCTTTGAAATCTGTGAAG+CGG - Chr2:53904233-53904252 MsG0280009535.01.T01:CDS 35.0%
AAACCATGAGAGTAGGGAAA+GGG - Chr2:53904375-53904394 MsG0280009535.01.T01:CDS 40.0%
AACCGCTTCACAGATTTCAA+AGG + Chr2:53904238-53904257 None:intergenic 40.0%
ACCGGAGTAATTCAGAACAT+TGG - Chr2:53905216-53905235 MsG0280009535.01.T01:CDS 40.0%
ATCCATGATTCATTGATCCC+GGG + Chr2:53905081-53905100 None:intergenic 40.0%
ATGGAAAGCTTGACACTCTT+TGG - Chr2:53904727-53904746 MsG0280009535.01.T01:intron 40.0%
ATTAAGGGGTTTATTGGAGC+AGG - Chr2:53905132-53905151 MsG0280009535.01.T01:CDS 40.0%
TAAACCATGAGAGTAGGGAA+AGG - Chr2:53904374-53904393 MsG0280009535.01.T01:CDS 40.0%
TCATGGTTTAGGAGGAAACA+TGG + Chr2:53904364-53904383 None:intergenic 40.0%
TGGATTAAGCTCTTCGATGT+TGG - Chr2:53905096-53905115 MsG0280009535.01.T01:CDS 40.0%
TTAAGGGGTTTATTGGAGCA+GGG - Chr2:53905133-53905152 MsG0280009535.01.T01:CDS 40.0%
TTTCCCTACTCTCATGGTTT+AGG + Chr2:53904375-53904394 None:intergenic 40.0%
! AGAACAAAGTGCCATTGAAC+TGG - Chr2:53904428-53904447 MsG0280009535.01.T01:CDS 40.0%
AAGCTTGACACTCTTTGGCA+TGG - Chr2:53904732-53904751 MsG0280009535.01.T01:intron 45.0%
ATATCAATGTTGGGTGAGCC+CGG - Chr2:53905060-53905079 MsG0280009535.01.T01:CDS 45.0%
CCTCCTAAACCATGAGAGTA+GGG - Chr2:53904369-53904388 MsG0280009535.01.T01:CDS 45.0%
CGGGAATAACACAATGATCG+TGG + Chr2:53904612-53904631 None:intergenic 45.0%
CGTTCTCGAATAAAAGGACG+TGG + Chr2:53904454-53904473 None:intergenic 45.0%
TAAGTCTGAGGGTAGGAAAG+TGG + Chr2:53904944-53904963 None:intergenic 45.0%
TCCTCCTAAACCATGAGAGT+AGG - Chr2:53904368-53904387 MsG0280009535.01.T01:CDS 45.0%
! AATCTGTTTTGCCCGACCAA+TGG + Chr2:53904873-53904892 None:intergenic 45.0%
! ATATGCGAGAGCGTACTCTT+CGG - Chr2:53904809-53904828 MsG0280009535.01.T01:CDS 45.0%
!! TTCGGTGTTCGGTAGGTATT+GGG - Chr2:53904827-53904846 MsG0280009535.01.T01:CDS 45.0%
AAAGGACGTGGCCAGTTCAA+TGG + Chr2:53904442-53904461 None:intergenic 50.0%
ACTCCCTTTCCCTACTCTCA+TGG + Chr2:53904381-53904400 None:intergenic 50.0%
ACTTGGATGGAGTGTGCCAT+TGG - Chr2:53904854-53904873 MsG0280009535.01.T01:CDS 50.0%
AGCCCGGGATCAATGAATCA+TGG - Chr2:53905076-53905095 MsG0280009535.01.T01:CDS 50.0%
CCCTACTCTCATGGTTTAGG+AGG + Chr2:53904372-53904391 None:intergenic 50.0%
GAGGGTAGGAAAGTGGTAAC+AGG + Chr2:53904937-53904956 None:intergenic 50.0%
GTTTATTGGAGCAGGGAGGA+AGG - Chr2:53905140-53905159 MsG0280009535.01.T01:CDS 50.0%
TATCAATGTTGGGTGAGCCC+GGG - Chr2:53905061-53905080 MsG0280009535.01.T01:CDS 50.0%
TCTTTGGCATGGCCCCTATT+GGG - Chr2:53904743-53904762 MsG0280009535.01.T01:intron 50.0%
! GGTATTGGGGTTCACTTGGA+TGG - Chr2:53904841-53904860 MsG0280009535.01.T01:CDS 50.0%
! TCGGTGTTCGGTAGGTATTG+GGG - Chr2:53904828-53904847 MsG0280009535.01.T01:CDS 50.0%
!! AGAGCGTACTCTTCGGTGTT+CGG - Chr2:53904816-53904835 MsG0280009535.01.T01:CDS 50.0%
!! CTTCGGTGTTCGGTAGGTAT+TGG - Chr2:53904826-53904845 MsG0280009535.01.T01:CDS 50.0%
!! GGTAGGTATTGGGGTTCACT+TGG - Chr2:53904837-53904856 MsG0280009535.01.T01:CDS 50.0%
AGGGGTTTATTGGAGCAGGG+AGG - Chr2:53905136-53905155 MsG0280009535.01.T01:CDS 55.0%
CTCTTTGGCATGGCCCCTAT+TGG - Chr2:53904742-53904761 MsG0280009535.01.T01:intron 55.0%
! GATGGAGTGTGCCATTGGTC+GGG - Chr2:53904859-53904878 MsG0280009535.01.T01:CDS 55.0%
! GGATGGAGTGTGCCATTGGT+CGG - Chr2:53904858-53904877 MsG0280009535.01.T01:CDS 55.0%
!! CGTACTCTTCGGTGTTCGGT+AGG - Chr2:53904820-53904839 MsG0280009535.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 53904163 53905317 53904163 ID=MsG0280009535.01;Name=MsG0280009535.01
Chr2 mRNA 53904163 53905317 53904163 ID=MsG0280009535.01.T01;Parent=MsG0280009535.01;Name=MsG0280009535.01.T01;_AED=0.19;_eAED=0.19;_QI=0|0|0|1|1|1|2|0|351
Chr2 exon 53904805 53905317 53904805 ID=MsG0280009535.01.T01:exon:18484;Parent=MsG0280009535.01.T01
Chr2 exon 53904163 53904705 53904163 ID=MsG0280009535.01.T01:exon:18485;Parent=MsG0280009535.01.T01
Chr2 CDS 53904805 53905317 53904805 ID=MsG0280009535.01.T01:cds;Parent=MsG0280009535.01.T01
Chr2 CDS 53904163 53904705 53904163 ID=MsG0280009535.01.T01:cds;Parent=MsG0280009535.01.T01
Gene Sequence

>MsG0280009535.01.T01

ATGGCTGCGATCAATGAAAAGGTTAGTATCTATGTTCCTGAAGATATCGTCTTCTCTATTCTTTCAAAACTACCTTTGAAATCTGTGAAGCGGTTTACTTGTGTAAACAAACATTGGAGTCATTTATTTGAAAACCCTTATTTCATGAACATGTTTTCCAAAAATATGGTTTCCAAATATAGTTCATTGTACGATGAACCATGTTTCCTCCTAAACCATGAGAGTAGGGAAAGGGAGTTGTATTTGATTTCTGGAAAGAGAATTGAGAACAAAGTGCCATTGAACTGGCCACGTCCTTTTATTCGAGAACGATATAATTATCCTTGTCCTTTTATTGCATGTTCATCTGTTAATGGCACTATTTGTATCTACGATGACATTGATAGTCATTCAACTGTTGTATTGTGGAATCCCGCTACCGATGAATTACATATTGTTGCTGAGGACCACGATCATTGTGTTATTCCCGATTTTTATAATGATAGAGAATTACCTATACTATTCATGGATTCGTTTAAAGGTAACTATTGGGATACACTCGATGTTGATATGCGAGAGCGTACTCTTCGGTGTTCGGTAGGTATTGGGGTTCACTTGGATGGAGTGTGCCATTGGTCGGGCAAAACAGATTGTGAAACATATGTGGTATCATTTAACTTGAGCACTGAAGTGCCTGTTACCACTTTCCTACCCTCAGACTTATATGATATACAATGGGTTGATAGATATTTGGCGGAGTTAAATGGGCATGTAATTATGATCTCCCAGTATGTAAAGACCACTTCTTTCCACATATCAATATCAATGTTGGGTGAGCCCGGGATCAATGAATCATGGATTAAGCTCTTCGATGTTGGACCCTTATCTGATATTAAGGGGTTTATTGGAGCAGGGAGGAAGGCCGATATATTTTTAAAAAAAGCTGATGATGAACTTGCATATTTTGATTTAACTACCGGAGTAATTCAGAACATTGGTGTTAAAGGAAAATCATTTTGTTCTCAAGTAGTATTTTATAAGAAAAACATTCTTCCAATCGTAGGAATCCAAAATTAA
Protein sequence

>MsG0280009535.01.T01

MAAINEKVSIYVPEDIVFSILSKLPLKSVKRFTCVNKHWSHLFENPYFMNMFSKNMVSKYSSLYDEPCFLLNHESRERELYLISGKRIENKVPLNWPRPFIRERYNYPCPFIACSSVNGTICIYDDIDSHSTVVLWNPATDELHIVAEDHDHCVIPDFYNDRELPILFMDSFKGNYWDTLDVDMRERTLRCSVGIGVHLDGVCHWSGKTDCETYVVSFNLSTEVPVTTFLPSDLYDIQWVDRYLAELNGHVIMISQYVKTTSFHISISMLGEPGINESWIKLFDVGPLSDIKGFIGAGRKADIFLKKADDELAYFDLTTGVIQNIGVKGKSFCSQVVFYKKNILPIVGIQN*