AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028261.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028261.01.T01 MTR_5g059430 47.343 414 119 13 17 341 16 419 1.16e-102 308
MsG0580028261.01.T01 MTR_5g037680 46.134 388 131 6 21 341 3 379 1.42e-101 306
MsG0580028261.01.T01 MTR_5g069390 45.729 398 131 11 21 341 21 410 9.04e-99 301
MsG0580028261.01.T01 MTR_0054s0090 38.425 419 155 12 11 344 2 402 1.27e-73 233
MsG0580028261.01.T01 MTR_5g066420 77.852 149 22 2 200 337 1 149 3.79e-73 223
MsG0580028261.01.T01 MTR_0054s0100 38.554 415 156 13 11 341 2 401 1.31e-72 231
MsG0580028261.01.T01 MTR_6g086825 39.098 399 147 10 25 341 69 453 4.15e-72 231
MsG0580028261.01.T01 MTR_2g038680 37.410 417 166 10 12 344 2 407 7.47e-70 224
MsG0580028261.01.T01 MTR_2g021800 38.861 404 157 12 25 344 15 412 7.03e-68 219
MsG0580028261.01.T01 MTR_8g063440 35.949 395 172 8 15 344 1 379 3.41e-67 216
MsG0580028261.01.T01 MTR_5g096630 36.145 415 169 13 15 344 7 410 1.49e-66 215
MsG0580028261.01.T01 MTR_5g097220 37.740 416 156 12 17 344 4 404 2.52e-66 214
MsG0580028261.01.T01 MTR_0134s0060 37.530 413 158 14 15 338 1 402 5.86e-66 214
MsG0580028261.01.T01 MTR_2g022000 38.519 405 156 13 25 344 15 411 6.09e-66 214
MsG0580028261.01.T01 MTR_4g130330 38.916 406 148 14 20 344 1 387 1.34e-65 212
MsG0580028261.01.T01 MTR_4g102410 36.203 395 169 10 25 344 60 446 4.57e-65 213
MsG0580028261.01.T01 MTR_0129s0070 37.817 394 144 12 25 339 16 387 1.43e-64 210
MsG0580028261.01.T01 MTR_5g097330 37.965 403 149 14 25 344 15 399 1.09e-62 205
MsG0580028261.01.T01 MTR_0129s0120 36.709 395 147 13 25 339 16 387 1.52e-62 204
MsG0580028261.01.T01 MTR_4g028510 39.231 390 159 14 26 344 16 398 1.96e-62 206
MsG0580028261.01.T01 MTR_6g478230 35.961 406 189 10 1 341 1 400 4.56e-62 204
MsG0580028261.01.T01 MTR_8g012090 37.602 367 124 9 15 316 5 331 9.63e-62 201
MsG0580028261.01.T01 MTR_5g091140 38.522 379 158 14 10 327 32 396 2.05e-61 202
MsG0580028261.01.T01 MTR_3g011120 36.436 376 159 12 25 338 13 370 4.65e-61 200
MsG0580028261.01.T01 MTR_6g090180 35.142 424 166 14 9 344 32 434 2.38e-60 200
MsG0580028261.01.T01 MTR_5g096910 37.913 393 152 13 25 344 16 389 2.60e-60 199
MsG0580028261.01.T01 MTR_0129s0050 36.973 403 150 14 25 339 13 399 4.53e-60 198
MsG0580028261.01.T01 MTR_2g021770 36.070 402 164 11 26 344 12 403 4.82e-60 198
MsG0580028261.01.T01 MTR_5g092850 36.430 409 164 15 16 344 6 398 7.32e-59 195
MsG0580028261.01.T01 MTR_2g021950 35.732 403 171 12 17 344 4 393 7.68e-59 195
MsG0580028261.01.T01 MTR_4g028680 38.213 403 167 15 11 344 7 396 1.45e-58 194
MsG0580028261.01.T01 MTR_6g079770 36.946 406 162 12 25 344 16 413 2.24e-58 194
MsG0580028261.01.T01 MTR_4g028660 36.935 398 172 13 11 341 7 392 7.28e-58 192
MsG0580028261.01.T01 MTR_4g027450 36.935 398 172 13 11 341 7 392 7.28e-58 192
MsG0580028261.01.T01 MTR_6g461660 36.298 416 160 12 11 344 4 396 1.43e-57 192
MsG0580028261.01.T01 MTR_4g055820 36.314 369 144 10 16 306 4 359 1.48e-56 188
MsG0580028261.01.T01 MTR_5g097680 35.198 429 176 14 11 344 2 423 4.60e-56 188
MsG0580028261.01.T01 MTR_7g079640 36.559 372 151 16 17 325 4 353 6.71e-56 187
MsG0580028261.01.T01 MTR_0831s0010 33.663 404 181 12 9 344 33 417 1.22e-55 187
MsG0580028261.01.T01 MTR_4g028750 36.318 402 177 13 15 343 1 396 3.04e-55 186
MsG0580028261.01.T01 MTR_5g074850 37.374 396 143 17 16 325 25 401 7.53e-54 190
MsG0580028261.01.T01 MTR_5g092860 36.667 360 140 13 44 335 20 359 8.35e-54 181
MsG0580028261.01.T01 MTR_4g117550 35.770 383 148 14 25 341 16 366 8.41e-54 181
MsG0580028261.01.T01 MTR_2g066200 33.831 402 176 17 15 344 1 384 1.80e-52 178
MsG0580028261.01.T01 MTR_8g046220 33.747 403 162 10 25 341 16 399 2.88e-52 178
MsG0580028261.01.T01 MTR_5g097350 36.508 378 153 16 35 344 25 383 3.14e-52 177
MsG0580028261.01.T01 MTR_8g046310 35.279 377 164 12 25 341 15 371 7.73e-52 176
MsG0580028261.01.T01 MTR_8g464770 34.211 380 170 9 25 341 11 373 1.14e-51 176
MsG0580028261.01.T01 MTR_8g064890 34.884 387 168 10 25 344 15 384 1.29e-51 176
MsG0580028261.01.T01 MTR_1g007410 33.173 416 182 16 8 344 4 402 5.41e-51 174
MsG0580028261.01.T01 MTR_2g034700 35.013 377 165 11 25 341 13 369 7.15e-51 173
MsG0580028261.01.T01 MTR_1g007450 33.924 395 171 15 15 334 3 382 1.92e-50 174
MsG0580028261.01.T01 MTR_7g069550 34.121 381 149 12 26 323 14 375 2.53e-50 172
MsG0580028261.01.T01 MTR_5g075370 37.084 391 140 18 25 327 7 379 5.26e-50 178
MsG0580028261.01.T01 MTR_7g017130 35.417 384 165 12 26 339 18 388 1.61e-49 171
MsG0580028261.01.T01 MTR_2g064950 35.340 382 155 14 15 322 1 364 2.18e-49 170
MsG0580028261.01.T01 MTR_7g017170 36.216 370 153 12 26 325 18 374 3.19e-49 170
MsG0580028261.01.T01 MTR_7g017040 32.658 395 176 10 25 344 15 394 3.62e-49 169
MsG0580028261.01.T01 MTR_8g041270 33.252 412 172 10 19 344 8 402 3.65e-49 170
MsG0580028261.01.T01 MTR_2g066170 32.161 398 180 14 15 341 1 379 3.71e-49 169
MsG0580028261.01.T01 MTR_4g014370 36.336 333 179 10 25 344 21 333 1.09e-48 167
MsG0580028261.01.T01 MTR_1g098180 33.333 378 171 12 19 343 12 361 6.18e-48 166
MsG0580028261.01.T01 MTR_6g078010 45.291 223 99 7 131 341 117 328 1.08e-47 164
MsG0580028261.01.T01 MTR_8g467670 32.812 384 173 11 25 344 13 375 1.44e-47 165
MsG0580028261.01.T01 MTR_1g053510 32.936 419 169 16 15 341 1 399 3.25e-47 165
MsG0580028261.01.T01 MTR_2g104340 32.924 407 171 12 19 344 7 392 5.71e-47 164
MsG0580028261.01.T01 MTR_5g097820 36.957 276 98 7 12 226 9 269 8.28e-47 160
MsG0580028261.01.T01 MTR_4g014370 33.666 401 180 15 25 344 10 405 5.51e-46 161
MsG0580028261.01.T01 MTR_6g080680 45.045 222 99 8 132 341 178 388 5.80e-46 161
MsG0580028261.01.T01 MTR_6g080680 37.333 150 70 5 10 155 2 131 4.86e-14 72.8
MsG0580028261.01.T01 MTR_8g046200 33.495 412 166 15 25 340 15 414 7.57e-46 161
MsG0580028261.01.T01 MTR_8g039500 32.493 357 160 9 15 310 1 337 7.65e-46 159
MsG0580028261.01.T01 MTR_8g063330 30.919 359 159 7 19 305 7 348 1.50e-45 162
MsG0580028261.01.T01 MTR_8g032980 32.644 435 156 15 19 343 9 416 1.98e-45 160
MsG0580028261.01.T01 MTR_6g079810 41.277 235 102 5 139 344 232 459 6.43e-45 160
MsG0580028261.01.T01 MTR_6g079810 37.821 156 69 6 3 148 40 177 8.01e-18 84.7
MsG0580028261.01.T01 MTR_2g065860 30.769 403 187 12 15 344 1 384 1.28e-44 157
MsG0580028261.01.T01 MTR_6g090260 46.316 190 83 5 164 341 75 257 1.83e-44 154
MsG0580028261.01.T01 MTR_6g090260 56.667 60 26 0 16 75 10 69 1.26e-11 64.7
MsG0580028261.01.T01 MTR_2g104280 32.518 409 179 13 19 344 7 401 2.70e-44 157
MsG0580028261.01.T01 MTR_5g043605 33.616 354 183 12 8 339 7 330 1.34e-43 154
MsG0580028261.01.T01 MTR_8g033110 29.508 427 182 11 16 343 4 410 1.77e-43 155
MsG0580028261.01.T01 MTR_5g097850 46.632 193 72 6 169 344 63 241 4.45e-43 149
MsG0580028261.01.T01 MTR_6g004450 41.810 232 108 5 131 344 43 265 4.48e-43 150
MsG0580028261.01.T01 MTR_5g072060 33.889 360 160 11 25 323 5 347 5.25e-43 157
MsG0580028261.01.T01 MTR_2g066150 31.633 392 170 12 15 344 1 356 6.96e-43 152
MsG0580028261.01.T01 MTR_8g033140 30.913 427 168 13 19 343 12 413 6.97e-43 154
MsG0580028261.01.T01 MTR_8g036970 35.928 334 164 16 30 335 2 313 1.39e-42 152
MsG0580028261.01.T01 MTR_8g064790 33.621 348 175 12 25 344 14 333 1.63e-42 150
MsG0580028261.01.T01 MTR_8g018110 30.024 413 180 12 16 343 4 392 3.45e-42 151
MsG0580028261.01.T01 MTR_8g013000 32.908 392 156 13 18 325 1 369 1.77e-41 149
MsG0580028261.01.T01 MTR_7g111700 32.727 385 157 13 25 344 14 361 1.97e-41 148
MsG0580028261.01.T01 MTR_5g086590 43.119 218 90 8 128 327 34 235 4.00e-41 144
MsG0580028261.01.T01 MTR_6g079790 40.343 233 102 6 139 341 77 302 1.53e-40 144
MsG0580028261.01.T01 MTR_6g090250 38.843 242 102 7 139 341 183 417 2.78e-40 147
MsG0580028261.01.T01 MTR_6g087620 42.105 228 86 7 130 338 79 279 1.09e-39 141
MsG0580028261.01.T01 MTR_5g064700 40.851 235 101 9 139 344 139 364 5.98e-39 142
MsG0580028261.01.T01 MTR_5g022850 31.653 357 193 12 21 344 11 349 1.03e-38 141
MsG0580028261.01.T01 MTR_5g097540 37.209 258 137 6 63 314 1 239 3.72e-38 138
MsG0580028261.01.T01 MTR_1g007430 33.429 350 123 12 37 308 7 324 4.35e-38 139
MsG0580028261.01.T01 MTR_7g104710 36.301 292 110 9 25 263 16 284 1.53e-36 134
MsG0580028261.01.T01 MTR_3g018750 39.252 214 101 7 139 325 5 216 1.87e-36 132
MsG0580028261.01.T01 MTR_4g007940 30.330 333 135 10 95 344 20 338 3.00e-36 134
MsG0580028261.01.T01 MTR_2g021920 38.000 250 110 8 127 344 33 269 6.43e-36 131
MsG0580028261.01.T01 MTR_8g063350 33.533 334 157 15 25 321 13 318 8.56e-36 133
MsG0580028261.01.T01 MTR_5g094350 38.462 273 98 11 25 239 12 272 6.00e-35 129
MsG0580028261.01.T01 MTR_7g016930 34.551 301 142 10 25 281 15 304 9.83e-35 130
MsG0580028261.01.T01 MTR_8g033090 29.469 414 178 15 25 343 13 407 3.19e-34 130
MsG0580028261.01.T01 MTR_5g076490 34.957 349 143 13 63 344 1 332 3.93e-34 128
MsG0580028261.01.T01 MTR_2g065840 30.667 375 185 13 15 325 1 364 5.93e-34 129
MsG0580028261.01.T01 MTR_5g023970 40.306 196 99 5 154 340 1 187 6.40e-34 124
MsG0580028261.01.T01 MTR_6g079730 46.853 143 66 2 209 341 1 143 9.05e-34 122
MsG0580028261.01.T01 MTR_5g097660 64.286 98 32 2 245 339 14 111 1.13e-33 121
MsG0580028261.01.T01 MTR_5g074800 31.006 358 143 13 65 340 1 336 1.24e-33 127
MsG0580028261.01.T01 MTR_8g063370 31.367 373 176 14 30 339 28 383 2.79e-32 124
MsG0580028261.01.T01 MTR_5g096920 44.156 154 71 4 200 344 1 148 6.42e-32 117
MsG0580028261.01.T01 MTR_6g009350 40.107 187 91 5 139 309 10 191 8.83e-32 118
MsG0580028261.01.T01 MTR_7g023800 40.110 182 86 5 175 344 137 307 2.88e-31 120
MsG0580028261.01.T01 MTR_8g035690 39.683 189 90 5 165 344 126 299 5.76e-29 114
MsG0580028261.01.T01 MTR_1g076810 37.089 213 72 7 25 180 17 224 1.08e-28 117
MsG0580028261.01.T01 MTR_1g050312 33.449 287 103 11 102 327 15 274 1.13e-28 112
MsG0580028261.01.T01 MTR_5g068490 34.286 210 118 5 139 341 74 270 2.24e-28 111
MsG0580028261.01.T01 MTR_5g097650 45.270 148 57 4 17 164 13 136 1.13e-27 106
MsG0580028261.01.T01 MTR_3g030620 41.975 162 75 7 195 341 18 175 1.88e-26 103
MsG0580028261.01.T01 MTR_2g022020 56.863 102 40 2 245 344 17 116 3.94e-26 100
MsG0580028261.01.T01 MTR_8g033000 37.374 198 91 7 164 343 176 358 8.51e-26 106
MsG0580028261.01.T01 MTR_7g101200 31.900 279 128 13 40 307 1 228 7.48e-25 101
MsG0580028261.01.T01 MTR_7g019250 33.333 216 91 5 139 327 226 415 1.41e-24 104
MsG0580028261.01.T01 MTR_8g063310 27.621 391 206 14 19 344 15 393 1.74e-24 103
MsG0580028261.01.T01 MTR_7g017150 41.520 171 80 5 164 324 82 242 1.79e-24 100
MsG0580028261.01.T01 MTR_4g016000 33.333 204 91 7 121 300 8 190 6.30e-24 98.2
MsG0580028261.01.T01 MTR_1g115205 33.061 245 104 9 7 209 12 238 1.51e-23 98.2
MsG0580028261.01.T01 MTR_7g017120 40.351 171 82 5 164 324 81 241 2.16e-23 97.8
MsG0580028261.01.T01 MTR_1g050308 33.906 233 95 8 1 180 9 235 2.69e-23 99.4
MsG0580028261.01.T01 MTR_1g050308 42.718 103 52 3 246 344 244 343 7.66e-13 68.9
MsG0580028261.01.T01 MTR_5g098950 37.912 182 71 5 139 308 123 274 7.29e-23 96.7
MsG0580028261.01.T01 MTR_8g073570 49.474 95 41 2 25 119 9 96 1.06e-21 90.9
MsG0580028261.01.T01 MTR_5g024260 32.432 222 107 8 139 330 180 388 3.52e-21 94.0
MsG0580028261.01.T01 MTR_5g024260 47.222 108 44 2 11 118 13 107 5.64e-21 93.6
MsG0580028261.01.T01 MTR_6g465360 41.212 165 74 8 1 160 35 181 4.34e-20 89.4
MsG0580028261.01.T01 MTR_6g070880 38.750 160 77 7 5 162 11 151 2.50e-19 86.7
MsG0580028261.01.T01 MTR_6g071420 38.750 160 77 7 5 162 11 151 2.50e-19 86.7
MsG0580028261.01.T01 MTR_5g096050 48.077 104 39 3 200 288 1 104 5.34e-19 82.0
MsG0580028261.01.T01 MTR_5g096900 39.735 151 66 5 136 273 175 313 8.05e-19 86.3
MsG0580028261.01.T01 MTR_5g096900 51.000 100 40 2 20 118 6 97 3.29e-18 84.3
MsG0580028261.01.T01 MTR_5g036250 54.430 79 33 2 263 339 25 102 2.31e-18 82.0
MsG0580028261.01.T01 MTR_5g086780 44.762 105 48 2 230 324 124 228 8.64e-18 81.6
MsG0580028261.01.T01 MTR_5g086780 34.868 152 47 5 10 151 2 111 1.02e-11 64.3
MsG0580028261.01.T01 MTR_8g041440 55.128 78 33 1 15 92 6 81 2.10e-17 77.4
MsG0580028261.01.T01 MTR_3g019290 36.111 144 80 3 17 151 5 145 4.01e-17 78.6
MsG0580028261.01.T01 MTR_5g037190 41.221 131 64 4 26 156 16 133 6.64e-17 79.7
MsG0580028261.01.T01 MTR_3g103020 24.274 379 229 15 10 344 7 371 1.61e-14 74.3
MsG0580028261.01.T01 MTR_4g094400 40.952 105 42 3 199 284 9 112 1.14e-13 67.8
MsG0580028261.01.T01 MTR_5g096070 55.172 58 24 1 13 68 11 68 2.35e-13 67.4
MsG0580028261.01.T01 MTR_8g032390 24.719 356 220 19 18 344 32 368 2.54e-13 70.5
MsG0580028261.01.T01 MTR_2g461450 22.807 342 208 11 12 308 4 334 4.58e-12 66.6
MsG0580028261.01.T01 MTR_6g087660 41.818 110 54 3 10 116 2 104 5.97e-12 64.3
MsG0580028261.01.T01 MTR_5g097730 67.500 40 13 0 272 311 15 54 1.88e-11 59.3
MsG0580028261.01.T01 MTR_1g068875 22.647 340 202 14 29 320 7 333 1.91e-11 65.1
MsG0580028261.01.T01 MTR_2g028110 23.167 341 204 14 29 321 7 337 3.24e-11 64.3
MsG0580028261.01.T01 MTR_3g047580 42.857 63 36 0 25 87 13 75 6.53e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 65 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCATTTGGTACTATTAAA+TGG 0.115472 5:+77409459 MsG0580028261.01.T01:CDS
CTCAAATGTATCATAGTAAT+AGG 0.233333 5:-77408925 None:intergenic
GCGGTAGACGTTCATGAAAA+TGG 0.260740 5:-77408820 None:intergenic
ATAGTTTAAGAAGTAATTCT+TGG 0.264187 5:+77409229 MsG0580028261.01.T01:CDS
TACAATTCAAATTTCAATCT+TGG 0.264647 5:+77409518 MsG0580028261.01.T01:CDS
AGAATAGGGTCAAATTAAAC+TGG 0.271848 5:+77408976 MsG0580028261.01.T01:CDS
AATCGCTTGAAAGATTTAAT+AGG 0.281695 5:-77408756 None:intergenic
CACCAAGGTCCTCCATCATT+TGG 0.345498 5:-77409680 None:intergenic
TAGTGAGAAGTTTCAGAATA+GGG 0.353697 5:+77408962 MsG0580028261.01.T01:CDS
TATGTTTCTCATCGTAAGTT+TGG 0.354346 5:+77409126 MsG0580028261.01.T01:CDS
ATAGATTTATCCTGGTAATA+AGG 0.354739 5:-77408867 None:intergenic
ACAATTCAAATTTCAATCTT+GGG 0.373995 5:+77409519 MsG0580028261.01.T01:CDS
CTAGTGAGAAGTTTCAGAAT+AGG 0.385143 5:+77408961 MsG0580028261.01.T01:CDS
ATTTCAATCTTGGGTGAACT+TGG 0.403095 5:+77409528 MsG0580028261.01.T01:CDS
ACCAAGGTCCTCCATCATTT+GGG 0.410615 5:-77409679 None:intergenic
ATCATTTGGTACTATTAAAT+GGG 0.421447 5:+77409460 MsG0580028261.01.T01:CDS
ATTAAATGGGTCTATTGCTC+TGG 0.426185 5:+77409473 MsG0580028261.01.T01:CDS
ACTTGACAGTGCTGAGGGTA+TGG 0.426699 5:-77409436 None:intergenic
TCAAATGTATCATAGTAATA+GGG 0.429935 5:-77408924 None:intergenic
AGCACTGTCAAGTGATCATT+TGG 0.431957 5:+77409446 MsG0580028261.01.T01:CDS
CGGTAGACGTTCATGAAAAT+GGG 0.444062 5:-77408819 None:intergenic
ACCCTTCTTTCCTACTCCGA+TGG 0.463256 5:-77409610 None:intergenic
AATGCTTGTTGTCATTTGAC+TGG 0.470350 5:+77409388 MsG0580028261.01.T01:CDS
TCATGAAGAAAGACGGCGGC+TGG 0.477391 5:+77409643 MsG0580028261.01.T01:CDS
ACATAGAAATTTAAATCATG+TGG 0.485258 5:+77408896 MsG0580028261.01.T01:CDS
CTTGGGTGAACTTGGTGTAA+AGG 0.486647 5:+77409536 MsG0580028261.01.T01:CDS
AACTTGGTGTAAAGGAATCA+TGG 0.495770 5:+77409544 MsG0580028261.01.T01:CDS
ACATGGATGGACTCTCTCAC+TGG 0.496194 5:+77409325 MsG0580028261.01.T01:CDS
CTTCCATGTATATGTTCCTA+AGG 0.506640 5:-77409202 None:intergenic
CCCTTCTTTCCTACTCCGAT+GGG 0.512120 5:-77409609 None:intergenic
CGACTTAAATACCCAAATGA+TGG 0.513304 5:+77409668 MsG0580028261.01.T01:CDS
CCTTGCCTTTACTGTCCCAT+CGG 0.520808 5:+77409594 MsG0580028261.01.T01:CDS
TCCCATCGGAGTAGGAAAGA+AGG 0.521226 5:+77409608 MsG0580028261.01.T01:CDS
TGGATTAAAATCTTTGCTGT+AGG 0.521874 5:+77409564 MsG0580028261.01.T01:CDS
GTAAGAGAATAGATTTATCC+TGG 0.525904 5:-77408875 None:intergenic
CTTTATAGTCGTCGTTGACA+CGG 0.529440 5:-77409155 None:intergenic
AACTCGCGTCTGTAGAGGTA+AGG 0.533211 5:+77408682 MsG0580028261.01.T01:CDS
CTTTACTGTCCCATCGGAGT+AGG 0.535168 5:+77409600 MsG0580028261.01.T01:CDS
CTACTCCGATGGGACAGTAA+AGG 0.538416 5:-77409599 None:intergenic
TGCTCTGGTTGTAGTTTACA+CGG 0.561204 5:+77409488 MsG0580028261.01.T01:CDS
GACCTTGGTGTTACAAGCAT+CGG 0.570652 5:+77409693 MsG0580028261.01.T01:CDS
CATAGAAATTTAAATCATGT+GGG 0.574327 5:+77408897 MsG0580028261.01.T01:CDS
CCGCAAGACTTTCTTAACAA+AGG 0.574396 5:+77408839 MsG0580028261.01.T01:CDS
ATATTGAAGTGCATTGCACC+TGG 0.574773 5:+77409087 MsG0580028261.01.T01:CDS
ACAGAAACTCGCGTCTGTAG+AGG 0.578520 5:+77408677 MsG0580028261.01.T01:CDS
CTTGACAGTGCTGAGGGTAT+GGG 0.582250 5:-77409435 None:intergenic
ACTCCTTAGGAACATATACA+TGG 0.582867 5:+77409199 MsG0580028261.01.T01:CDS
CCCATCGGAGTAGGAAAGAA+GGG 0.585300 5:+77409609 MsG0580028261.01.T01:CDS
TGATCACTTGACAGTGCTGA+GGG 0.588684 5:-77409441 None:intergenic
GAATAGGGTCAAATTAAACT+GGG 0.591320 5:+77408977 MsG0580028261.01.T01:CDS
ACCCAAATGATGGAGGACCT+TGG 0.598884 5:+77409678 MsG0580028261.01.T01:CDS
AAAGGATCATCCTTATTACC+AGG 0.601465 5:+77408857 MsG0580028261.01.T01:CDS
GTCATTTGACTGGAGCAAAG+AGG 0.610970 5:+77409398 MsG0580028261.01.T01:CDS
AAATGGGATCATCAAACAAG+AGG 0.614973 5:-77408803 None:intergenic
ACACATGAAGGTGTAAAACA+TGG 0.625391 5:+77409363 MsG0580028261.01.T01:CDS
AATGGGATCATCAAACAAGA+GGG 0.625751 5:-77408802 None:intergenic
ATGTTGTTCATGAAGAAAGA+CGG 0.628205 5:+77409636 MsG0580028261.01.T01:CDS
CTTAAATACCCAAATGATGG+AGG 0.628335 5:+77409671 MsG0580028261.01.T01:CDS
TTGAATGCGTATGTAAATCA+CGG 0.633659 5:+77408778 MsG0580028261.01.T01:CDS
ATGATCACTTGACAGTGCTG+AGG 0.643187 5:-77409442 None:intergenic
CCGATGGGACAGTAAAGGCA+AGG 0.655773 5:-77409594 None:intergenic
TGTATAGCTGAAACACATGA+AGG 0.689591 5:+77409351 MsG0580028261.01.T01:CDS
GTCCGATGCTTGTAACACCA+AGG 0.695639 5:-77409695 None:intergenic
CCTTTGTTAAGAAAGTCTTG+CGG 0.733483 5:-77408839 None:intergenic
TTGTTCATGAAGAAAGACGG+CGG 0.744061 5:+77409639 MsG0580028261.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACATAAAAATCATTTCTA+GGG - Chr5:77409113-77409132 None:intergenic 15.0%
!! ATAAATGTAATATTGTATTG+TGG + Chr5:77409049-77409068 MsG0580028261.01.T01:intron 15.0%
!! ACAATTCAAATTTCAATCTT+GGG + Chr5:77409519-77409538 MsG0580028261.01.T01:CDS 20.0%
!! ACATAGAAATTTAAATCATG+TGG + Chr5:77408896-77408915 MsG0580028261.01.T01:CDS 20.0%
!! ATAGTTTAAGAAGTAATTCT+TGG + Chr5:77409229-77409248 MsG0580028261.01.T01:CDS 20.0%
!! ATCATTTGGTACTATTAAAT+GGG + Chr5:77409460-77409479 MsG0580028261.01.T01:CDS 20.0%
!! CATAGAAATTTAAATCATGT+GGG + Chr5:77408897-77408916 MsG0580028261.01.T01:CDS 20.0%
!! GAAACATAAAAATCATTTCT+AGG - Chr5:77409114-77409133 None:intergenic 20.0%
!! TACAATTCAAATTTCAATCT+TGG + Chr5:77409518-77409537 MsG0580028261.01.T01:CDS 20.0%
!! TCAAATGTATCATAGTAATA+GGG - Chr5:77408927-77408946 None:intergenic 20.0%
!!! ATTTAATAGGTAATTTTGAG+AGG - Chr5:77408746-77408765 None:intergenic 20.0%
!!! TAAACTGGGTTTAATATTTT+AGG + Chr5:77408991-77409010 MsG0580028261.01.T01:intron 20.0%
! AATCGCTTGAAAGATTTAAT+AGG - Chr5:77408759-77408778 None:intergenic 25.0%
! ACTTCAATATCTTCCTATAT+GGG - Chr5:77409077-77409096 None:intergenic 25.0%
! ATAGATTTATCCTGGTAATA+AGG - Chr5:77408870-77408889 None:intergenic 25.0%
! CTCAAATGTATCATAGTAAT+AGG - Chr5:77408928-77408947 None:intergenic 25.0%
! GATCATTTGGTACTATTAAA+TGG + Chr5:77409459-77409478 MsG0580028261.01.T01:CDS 25.0%
AATCAAAAATGGTACATGGA+TGG + Chr5:77409312-77409331 MsG0580028261.01.T01:CDS 30.0%
AGAATAGGGTCAAATTAAAC+TGG + Chr5:77408976-77408995 MsG0580028261.01.T01:CDS 30.0%
ATGTTGTTCATGAAGAAAGA+CGG + Chr5:77409636-77409655 MsG0580028261.01.T01:CDS 30.0%
CACTTCAATATCTTCCTATA+TGG - Chr5:77409078-77409097 None:intergenic 30.0%
CTTGTTGAAAAATACTCCTT+AGG + Chr5:77409186-77409205 MsG0580028261.01.T01:CDS 30.0%
GAATAGGGTCAAATTAAACT+GGG + Chr5:77408977-77408996 MsG0580028261.01.T01:CDS 30.0%
GTAAGAGAATAGATTTATCC+TGG - Chr5:77408878-77408897 None:intergenic 30.0%
TAGCAATCAAAAATGGTACA+TGG + Chr5:77409308-77409327 MsG0580028261.01.T01:CDS 30.0%
TAGTGAGAAGTTTCAGAATA+GGG + Chr5:77408962-77408981 MsG0580028261.01.T01:CDS 30.0%
TATGTTTCTCATCGTAAGTT+TGG + Chr5:77409126-77409145 MsG0580028261.01.T01:CDS 30.0%
TTAGCACTAGCAATCAAAAA+TGG + Chr5:77409301-77409320 MsG0580028261.01.T01:CDS 30.0%
TTGAATGCGTATGTAAATCA+CGG + Chr5:77408778-77408797 MsG0580028261.01.T01:CDS 30.0%
! TGGATTAAAATCTTTGCTGT+AGG + Chr5:77409564-77409583 MsG0580028261.01.T01:CDS 30.0%
!! AATAGGTAATTTTGAGAGGA+TGG - Chr5:77408742-77408761 None:intergenic 30.0%
!!! CTAAGGAGTATTTTTCAACA+AGG - Chr5:77409188-77409207 None:intergenic 30.0%
AAAGGATCATCCTTATTACC+AGG + Chr5:77408857-77408876 MsG0580028261.01.T01:CDS 35.0%
AAATGGGATCATCAAACAAG+AGG - Chr5:77408806-77408825 None:intergenic 35.0%
AATGGGATCATCAAACAAGA+GGG - Chr5:77408805-77408824 None:intergenic 35.0%
ACTCCTTAGGAACATATACA+TGG + Chr5:77409199-77409218 MsG0580028261.01.T01:CDS 35.0%
ATTAAATGGGTCTATTGCTC+TGG + Chr5:77409473-77409492 MsG0580028261.01.T01:CDS 35.0%
ATTTCAATCTTGGGTGAACT+TGG + Chr5:77409528-77409547 MsG0580028261.01.T01:CDS 35.0%
CCTTTGTTAAGAAAGTCTTG+CGG - Chr5:77408842-77408861 None:intergenic 35.0%
CGACTTAAATACCCAAATGA+TGG + Chr5:77409668-77409687 MsG0580028261.01.T01:CDS 35.0%
CTAGTGAGAAGTTTCAGAAT+AGG + Chr5:77408961-77408980 MsG0580028261.01.T01:CDS 35.0%
CTTAAATACCCAAATGATGG+AGG + Chr5:77409671-77409690 MsG0580028261.01.T01:CDS 35.0%
CTTCCATGTATATGTTCCTA+AGG - Chr5:77409205-77409224 None:intergenic 35.0%
TGTATAGCTGAAACACATGA+AGG + Chr5:77409351-77409370 MsG0580028261.01.T01:CDS 35.0%
TTGTATTGTGGAACCCATAT+AGG + Chr5:77409061-77409080 MsG0580028261.01.T01:intron 35.0%
! ACACATGAAGGTGTAAAACA+TGG + Chr5:77409363-77409382 MsG0580028261.01.T01:CDS 35.0%
!! AACTTGGTGTAAAGGAATCA+TGG + Chr5:77409544-77409563 MsG0580028261.01.T01:CDS 35.0%
!! AATGCTTGTTGTCATTTGAC+TGG + Chr5:77409388-77409407 MsG0580028261.01.T01:CDS 35.0%
!! TAAAAATCATTTCTAGGGCC+AGG - Chr5:77409108-77409127 None:intergenic 35.0%
AGCACTGTCAAGTGATCATT+TGG + Chr5:77409446-77409465 MsG0580028261.01.T01:CDS 40.0%
ATATTGAAGTGCATTGCACC+TGG + Chr5:77409087-77409106 MsG0580028261.01.T01:CDS 40.0%
CCGCAAGACTTTCTTAACAA+AGG + Chr5:77408839-77408858 MsG0580028261.01.T01:CDS 40.0%
CGGTAGACGTTCATGAAAAT+GGG - Chr5:77408822-77408841 None:intergenic 40.0%
CTTTATAGTCGTCGTTGACA+CGG - Chr5:77409158-77409177 None:intergenic 40.0%
TGCTCTGGTTGTAGTTTACA+CGG + Chr5:77409488-77409507 MsG0580028261.01.T01:CDS 40.0%
TTGTTCATGAAGAAAGACGG+CGG + Chr5:77409639-77409658 MsG0580028261.01.T01:CDS 40.0%
!! AATATTTTAGGCTCCGCTCA+TGG + Chr5:77409003-77409022 MsG0580028261.01.T01:intron 40.0%
ACCAAGGTCCTCCATCATTT+GGG - Chr5:77409682-77409701 None:intergenic 45.0%
ATGATCACTTGACAGTGCTG+AGG - Chr5:77409445-77409464 None:intergenic 45.0%
CAGACAAAGAGTTCCATGAG+CGG - Chr5:77409019-77409038 None:intergenic 45.0%
GCGGTAGACGTTCATGAAAA+TGG - Chr5:77408823-77408842 None:intergenic 45.0%
GTCATTTGACTGGAGCAAAG+AGG + Chr5:77409398-77409417 MsG0580028261.01.T01:CDS 45.0%
! TGATCACTTGACAGTGCTGA+GGG - Chr5:77409444-77409463 None:intergenic 45.0%
!! CTTGGGTGAACTTGGTGTAA+AGG + Chr5:77409536-77409555 MsG0580028261.01.T01:CDS 45.0%
!! GACCTTGGTGTTACAAGCAT+CGG + Chr5:77409693-77409712 MsG0580028261.01.T01:CDS 45.0%
AACTCGCGTCTGTAGAGGTA+AGG + Chr5:77408682-77408701 MsG0580028261.01.T01:CDS 50.0%
ACAGAAACTCGCGTCTGTAG+AGG + Chr5:77408677-77408696 MsG0580028261.01.T01:CDS 50.0%
ACCCTTCTTTCCTACTCCGA+TGG - Chr5:77409613-77409632 None:intergenic 50.0%
CACCAAGGTCCTCCATCATT+TGG - Chr5:77409683-77409702 None:intergenic 50.0%
CCCATCGGAGTAGGAAAGAA+GGG + Chr5:77409609-77409628 MsG0580028261.01.T01:CDS 50.0%
CCCTTCTTTCCTACTCCGAT+GGG - Chr5:77409612-77409631 None:intergenic 50.0%
CCTTGCCTTTACTGTCCCAT+CGG + Chr5:77409594-77409613 MsG0580028261.01.T01:CDS 50.0%
CTACTCCGATGGGACAGTAA+AGG - Chr5:77409602-77409621 None:intergenic 50.0%
CTTTACTGTCCCATCGGAGT+AGG + Chr5:77409600-77409619 MsG0580028261.01.T01:CDS 50.0%
GTCCGATGCTTGTAACACCA+AGG - Chr5:77409698-77409717 None:intergenic 50.0%
TCCCATCGGAGTAGGAAAGA+AGG + Chr5:77409608-77409627 MsG0580028261.01.T01:CDS 50.0%
! ACATGGATGGACTCTCTCAC+TGG + Chr5:77409325-77409344 MsG0580028261.01.T01:CDS 50.0%
! ACCCAAATGATGGAGGACCT+TGG + Chr5:77409678-77409697 MsG0580028261.01.T01:CDS 50.0%
! ACTTGACAGTGCTGAGGGTA+TGG - Chr5:77409439-77409458 None:intergenic 50.0%
! CTTGACAGTGCTGAGGGTAT+GGG - Chr5:77409438-77409457 None:intergenic 50.0%
CCGATGGGACAGTAAAGGCA+AGG - Chr5:77409597-77409616 None:intergenic 55.0%
TCATGAAGAAAGACGGCGGC+TGG + Chr5:77409643-77409662 MsG0580028261.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 77408642 77409764 77408642 ID=MsG0580028261.01;Name=MsG0580028261.01
Chr5 mRNA 77408642 77409764 77408642 ID=MsG0580028261.01.T01;Parent=MsG0580028261.01;Name=MsG0580028261.01.T01;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|344
Chr5 exon 77408642 77408998 77408642 ID=MsG0580028261.01.T01:exon:46333;Parent=MsG0580028261.01.T01
Chr5 exon 77409087 77409764 77409087 ID=MsG0580028261.01.T01:exon:46334;Parent=MsG0580028261.01.T01
Chr5 CDS 77408642 77408998 77408642 ID=MsG0580028261.01.T01:cds;Parent=MsG0580028261.01.T01
Chr5 CDS 77409087 77409764 77409087 ID=MsG0580028261.01.T01:cds;Parent=MsG0580028261.01.T01
Gene Sequence

>MsG0580028261.01.T01

ATGAAGAAAAAGCAGCTGCATAAGAAGAAGAAGAAACAGAAACTCGCGTCTGTAGAGGTAAGGAAACAACTATATATACATGATGATATTGCTTTCTCCATCCTCTCAAAATTACCTATTAAATCTTTCAAGCGATTTGAATGCGTATGTAAATCACGGTCCCTCTTGTTTGATGATCCCATTTTCATGAACGTCTACCGCAAGACTTTCTTAACAAAGGATCATCCTTATTACCAGGATAAATCTATTCTCTTACATAGAAATTTAAATCATGTGGGCTATCCCTATTACTATGATACATTTGAGTTTTATTCTCTTTCTAGTGAGAAGTTTCAGAATAGGGTCAAATTAAACTGGATATTGAAGTGCATTGCACCTGGCCCTAGAAATGATTTTTATGTTTCTCATCGTAAGTTTGGTTATGACCGTGTCAACGACGACTATAAAGTGTTGTGCCTTGTTGAAAAATACTCCTTAGGAACATATACATGGAAGATATATAGTTTAAGAAGTAATTCTTGGAGAAAACTTGATGTTGATGACAATATGCATAATTTGTATTTTAAAGAAATTAGCACTAGCAATCAAAAATGGTACATGGATGGACTCTCTCACTGGTTGTGTATAGCTGAAACACATGAAGGTGTAAAACATGGAGAATGCTTGTTGTCATTTGACTGGAGCAAAGAGGTTTTCATTATAACACCCATACCCTCAGCACTGTCAAGTGATCATTTGGTACTATTAAATGGGTCTATTGCTCTGGTTGTAGTTTACACGGCAACAACTACAATTCAAATTTCAATCTTGGGTGAACTTGGTGTAAAGGAATCATGGATTAAAATCTTTGCTGTAGGATCATTACCTTGCCTTTACTGTCCCATCGGAGTAGGAAAGAAGGGTGATATGTTGTTCATGAAGAAAGACGGCGGCTGGTTCGACTTAAATACCCAAATGATGGAGGACCTTGGTGTTACAAGCATCGGACTTCGTTGTCAAATAGTAATTCATAAAGAAAAGCTTTCTTCCATCTGA
Protein sequence

>MsG0580028261.01.T01

MKKKQLHKKKKKQKLASVEVRKQLYIHDDIAFSILSKLPIKSFKRFECVCKSRSLLFDDPIFMNVYRKTFLTKDHPYYQDKSILLHRNLNHVGYPYYYDTFEFYSLSSEKFQNRVKLNWILKCIAPGPRNDFYVSHRKFGYDRVNDDYKVLCLVEKYSLGTYTWKIYSLRSNSWRKLDVDDNMHNLYFKEISTSNQKWYMDGLSHWLCIAETHEGVKHGECLLSFDWSKEVFIITPIPSALSSDHLVLLNGSIALVVVYTATTTIQISILGELGVKESWIKIFAVGSLPCLYCPIGVGKKGDMLFMKKDGGWFDLNTQMMEDLGVTSIGLRCQIVIHKEKLSSI*