AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035812.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035812.01.T01 MTR_6g090250 65.676 370 96 7 1 342 56 422 4.89e-159 452
MsG0680035812.01.T01 MTR_6g090260 89.529 191 20 0 152 342 72 262 2.03e-126 363
MsG0680035812.01.T01 MTR_6g079810 56.495 331 125 7 20 339 136 458 6.83e-123 361
MsG0680035812.01.T01 MTR_6g078010 55.682 352 113 9 1 339 9 330 1.38e-122 357
MsG0680035812.01.T01 MTR_0134s0060 56.037 323 123 7 29 338 90 406 3.60e-115 340
MsG0680035812.01.T01 MTR_0054s0100 53.846 338 132 6 15 341 81 405 2.17e-114 338
MsG0680035812.01.T01 MTR_4g028510 52.676 355 133 9 1 339 62 397 6.27e-114 338
MsG0680035812.01.T01 MTR_0054s0090 52.959 338 133 6 15 341 81 403 3.14e-111 330
MsG0680035812.01.T01 MTR_6g079770 54.268 328 131 7 25 339 91 412 6.09e-111 329
MsG0680035812.01.T01 MTR_6g086825 53.333 330 114 7 30 341 150 457 6.27e-107 320
MsG0680035812.01.T01 MTR_4g130330 52.394 355 133 10 3 339 50 386 7.17e-106 315
MsG0680035812.01.T01 MTR_4g028680 50.141 355 141 10 1 339 61 395 1.67e-101 305
MsG0680035812.01.T01 MTR_4g028660 49.438 356 147 9 1 342 61 397 4.46e-101 303
MsG0680035812.01.T01 MTR_4g027450 49.438 356 147 9 1 342 61 397 4.46e-101 303
MsG0680035812.01.T01 MTR_6g079790 53.681 326 109 8 35 340 2 305 4.51e-100 298
MsG0680035812.01.T01 MTR_6g080680 49.858 351 133 7 4 338 66 389 1.36e-98 297
MsG0680035812.01.T01 MTR_6g090180 49.719 356 154 10 1 342 92 436 4.90e-97 294
MsG0680035812.01.T01 MTR_6g478230 49.275 345 120 10 18 341 94 404 3.00e-91 279
MsG0680035812.01.T01 MTR_6g087620 50.467 321 94 8 30 334 8 279 1.32e-89 270
MsG0680035812.01.T01 MTR_7g023800 45.428 339 119 6 15 342 26 309 8.38e-88 266
MsG0680035812.01.T01 MTR_6g079730 82.667 150 24 1 193 342 1 148 9.29e-86 255
MsG0680035812.01.T01 MTR_4g028750 48.494 332 137 10 3 319 63 375 9.37e-85 262
MsG0680035812.01.T01 MTR_2g022000 47.682 302 136 10 29 318 102 393 4.64e-77 243
MsG0680035812.01.T01 MTR_2g021800 44.648 327 159 9 20 336 94 408 5.20e-77 242
MsG0680035812.01.T01 MTR_5g097220 44.411 331 157 9 29 342 86 406 1.72e-75 238
MsG0680035812.01.T01 MTR_0129s0070 43.030 330 157 9 19 339 84 391 1.05e-73 233
MsG0680035812.01.T01 MTR_8g063440 43.125 320 142 11 29 339 90 378 2.12e-72 229
MsG0680035812.01.T01 MTR_0129s0120 42.042 333 162 9 19 342 84 394 1.18e-71 228
MsG0680035812.01.T01 MTR_6g461660 43.692 325 156 8 4 318 69 376 5.84e-71 226
MsG0680035812.01.T01 MTR_5g037680 43.252 326 133 11 29 338 91 380 5.20e-70 225
MsG0680035812.01.T01 MTR_2g038680 40.503 358 182 11 1 339 61 406 1.55e-68 220
MsG0680035812.01.T01 MTR_5g097330 43.034 323 158 10 29 342 96 401 7.52e-68 218
MsG0680035812.01.T01 MTR_4g102410 42.284 324 156 12 30 341 143 447 1.81e-66 216
MsG0680035812.01.T01 MTR_5g069390 40.909 330 159 10 25 340 106 413 5.03e-66 216
MsG0680035812.01.T01 MTR_7g104710 48.638 257 80 5 9 257 74 286 1.45e-65 210
MsG0680035812.01.T01 MTR_0129s0050 40.683 322 166 10 29 336 90 400 6.78e-64 208
MsG0680035812.01.T01 MTR_5g096630 39.942 343 147 11 29 341 98 411 2.25e-63 207
MsG0680035812.01.T01 MTR_4g117550 39.319 323 151 9 19 338 87 367 2.54e-63 206
MsG0680035812.01.T01 MTR_4g007940 39.806 309 149 9 30 322 35 322 2.92e-63 204
MsG0680035812.01.T01 MTR_5g097680 41.092 348 161 11 22 339 89 422 3.46e-63 207
MsG0680035812.01.T01 MTR_2g104340 43.533 317 158 9 30 338 87 390 1.64e-62 204
MsG0680035812.01.T01 MTR_7g069550 40.606 330 148 14 25 336 92 391 7.68e-62 202
MsG0680035812.01.T01 MTR_2g021950 42.767 318 155 12 29 339 95 392 3.24e-61 201
MsG0680035812.01.T01 MTR_5g064700 38.439 346 173 10 20 339 32 363 5.69e-61 199
MsG0680035812.01.T01 MTR_4g055820 41.935 279 141 8 29 298 93 359 1.18e-60 198
MsG0680035812.01.T01 MTR_5g097350 40.379 317 152 11 29 342 103 385 1.75e-60 199
MsG0680035812.01.T01 MTR_5g074800 41.538 325 164 12 20 336 30 336 2.14e-60 197
MsG0680035812.01.T01 MTR_5g092850 38.788 330 168 8 29 342 89 400 5.65e-60 198
MsG0680035812.01.T01 MTR_8g064890 38.365 318 163 9 29 339 92 383 1.17e-59 197
MsG0680035812.01.T01 MTR_2g021770 39.940 333 175 11 17 339 85 402 1.91e-59 197
MsG0680035812.01.T01 MTR_8g464770 40.557 323 158 10 20 338 82 374 5.84e-59 194
MsG0680035812.01.T01 MTR_2g065840 38.080 323 162 10 29 339 88 384 1.08e-58 194
MsG0680035812.01.T01 MTR_5g043605 36.709 316 166 7 29 340 50 335 2.20e-58 193
MsG0680035812.01.T01 MTR_8g041270 39.628 323 148 10 27 325 89 388 2.53e-58 194
MsG0680035812.01.T01 MTR_7g017130 40.923 325 167 11 20 339 88 392 7.26e-58 192
MsG0680035812.01.T01 MTR_5g092860 41.584 303 135 10 30 318 75 349 3.69e-57 189
MsG0680035812.01.T01 MTR_2g065860 38.509 322 161 10 29 339 88 383 3.71e-57 190
MsG0680035812.01.T01 MTR_8g012090 37.267 322 143 8 1 311 61 334 8.42e-57 187
MsG0680035812.01.T01 MTR_2g104280 39.799 299 158 6 30 315 90 379 2.79e-56 188
MsG0680035812.01.T01 MTR_5g059430 38.855 332 157 11 31 341 117 423 3.25e-56 189
MsG0680035812.01.T01 MTR_7g017170 41.914 303 153 10 20 317 88 372 4.40e-56 188
MsG0680035812.01.T01 MTR_5g096910 37.500 328 162 11 25 339 91 388 7.32e-56 187
MsG0680035812.01.T01 MTR_2g066170 36.677 319 168 13 29 339 89 381 3.56e-55 185
MsG0680035812.01.T01 MTR_7g079640 40.190 316 135 15 14 315 74 349 9.13e-55 184
MsG0680035812.01.T01 MTR_5g076490 41.975 324 146 13 29 339 37 331 1.27e-54 182
MsG0680035812.01.T01 MTR_8g467670 37.793 299 149 9 30 322 92 359 1.41e-54 184
MsG0680035812.01.T01 MTR_0831s0010 36.336 333 168 12 17 339 118 416 2.67e-54 184
MsG0680035812.01.T01 MTR_1g007450 39.806 309 164 10 18 318 78 372 5.42e-54 183
MsG0680035812.01.T01 MTR_2g066200 37.654 324 164 13 29 341 89 385 1.78e-53 181
MsG0680035812.01.T01 MTR_7g017040 36.441 354 191 11 3 342 63 396 2.46e-53 181
MsG0680035812.01.T01 MTR_2g021920 44.770 239 121 5 105 339 37 268 3.20e-53 176
MsG0680035812.01.T01 MTR_5g074850 40.127 314 149 13 29 322 110 404 5.12e-53 187
MsG0680035812.01.T01 MTR_5g068490 35.826 321 155 12 23 340 1 273 6.90e-53 176
MsG0680035812.01.T01 MTR_2g034700 36.795 337 164 12 15 342 78 374 2.16e-52 177
MsG0680035812.01.T01 MTR_5g075370 39.432 317 150 13 29 322 83 380 4.60e-52 184
MsG0680035812.01.T01 MTR_1g007410 38.245 319 160 13 21 325 94 389 7.09e-52 177
MsG0680035812.01.T01 MTR_6g004450 43.333 240 113 7 104 341 48 266 1.21e-51 172
MsG0680035812.01.T01 MTR_8g033140 35.030 334 178 9 29 340 98 414 1.24e-51 176
MsG0680035812.01.T01 MTR_3g011120 38.511 309 154 12 30 333 92 369 2.72e-51 174
MsG0680035812.01.T01 MTR_1g050312 37.107 318 138 12 29 335 22 288 1.91e-50 170
MsG0680035812.01.T01 MTR_5g086590 39.464 261 130 6 62 322 4 236 3.62e-50 167
MsG0680035812.01.T01 MTR_2g066150 38.390 323 140 15 29 341 84 357 1.15e-49 170
MsG0680035812.01.T01 MTR_3g018750 41.767 249 124 6 104 342 2 239 3.19e-49 165
MsG0680035812.01.T01 MTR_4g014370 35.503 338 166 11 31 341 94 406 5.67e-49 169
MsG0680035812.01.T01 MTR_5g097850 37.966 295 102 8 54 339 18 240 9.44e-49 164
MsG0680035812.01.T01 MTR_5g022850 37.931 319 169 12 30 338 48 347 1.12e-48 167
MsG0680035812.01.T01 MTR_5g072060 39.088 307 145 10 20 317 74 347 1.31e-48 172
MsG0680035812.01.T01 MTR_7g111700 37.994 329 151 11 22 339 74 360 1.39e-48 167
MsG0680035812.01.T01 MTR_1g053510 37.273 330 168 13 29 340 94 402 8.72e-48 166
MsG0680035812.01.T01 MTR_2g064950 37.662 308 157 11 20 315 79 363 1.58e-47 165
MsG0680035812.01.T01 MTR_8g039500 37.075 294 144 13 29 311 83 346 1.61e-47 164
MsG0680035812.01.T01 MTR_8g013000 39.803 304 145 11 4 297 75 350 1.80e-47 165
MsG0680035812.01.T01 MTR_4g016000 41.518 224 95 6 76 295 2 193 4.86e-47 159
MsG0680035812.01.T01 MTR_5g091140 38.742 302 130 11 29 315 128 389 7.49e-47 164
MsG0680035812.01.T01 MTR_5g023970 47.668 193 95 4 150 341 6 193 1.31e-46 157
MsG0680035812.01.T01 MTR_5g024260 34.545 330 161 13 30 331 99 401 1.39e-46 163
MsG0680035812.01.T01 MTR_8g046200 32.115 383 197 14 1 340 56 418 5.88e-46 162
MsG0680035812.01.T01 MTR_8g018110 35.258 329 169 11 30 341 93 394 2.13e-45 159
MsG0680035812.01.T01 MTR_8g063370 35.517 290 153 11 53 336 123 384 6.20e-45 158
MsG0680035812.01.T01 MTR_8g046310 33.803 355 175 14 3 340 63 374 1.95e-44 157
MsG0680035812.01.T01 MTR_6g070880 46.199 171 80 4 13 175 93 259 9.65e-44 152
MsG0680035812.01.T01 MTR_6g071420 46.199 171 80 4 13 175 93 259 9.65e-44 152
MsG0680035812.01.T01 MTR_8g032980 33.913 345 182 13 29 340 86 417 2.21e-43 155
MsG0680035812.01.T01 MTR_8g063350 35.811 296 149 12 31 315 53 318 2.87e-43 152
MsG0680035812.01.T01 MTR_8g033090 34.986 363 194 16 2 341 66 409 1.07e-42 153
MsG0680035812.01.T01 MTR_1g007430 37.234 282 149 7 18 295 62 319 1.36e-42 150
MsG0680035812.01.T01 MTR_8g033000 32.698 367 171 13 19 340 24 359 3.66e-42 150
MsG0680035812.01.T01 MTR_8g033110 35.962 317 154 13 52 341 118 412 6.90e-42 150
MsG0680035812.01.T01 MTR_8g063330 35.106 282 140 11 30 297 96 348 1.22e-41 151
MsG0680035812.01.T01 MTR_4g094400 65.714 105 24 2 182 274 6 110 2.82e-40 138
MsG0680035812.01.T01 MTR_7g016930 38.866 247 129 7 30 273 77 304 1.26e-39 143
MsG0680035812.01.T01 MTR_7g019250 41.256 223 98 6 103 321 222 415 1.43e-39 145
MsG0680035812.01.T01 MTR_5g098950 37.037 297 126 13 13 297 24 271 6.47e-39 139
MsG0680035812.01.T01 MTR_5g066420 49.342 152 66 4 186 331 1 147 1.02e-38 135
MsG0680035812.01.T01 MTR_1g098180 34.615 286 128 10 71 340 120 362 3.67e-38 140
MsG0680035812.01.T01 MTR_8g063310 31.562 320 178 11 30 336 98 389 1.47e-37 139
MsG0680035812.01.T01 MTR_4g014370 41.361 191 98 4 155 341 154 334 1.62e-37 137
MsG0680035812.01.T01 MTR_8g046220 29.973 367 204 16 1 340 62 402 2.89e-37 138
MsG0680035812.01.T01 MTR_3g030620 44.706 170 85 5 174 338 11 176 4.35e-37 132
MsG0680035812.01.T01 MTR_6g009350 40.887 203 101 6 106 305 9 195 4.74e-37 132
MsG0680035812.01.T01 MTR_8g036970 44.134 179 88 5 155 332 147 314 9.35e-37 136
MsG0680035812.01.T01 MTR_7g017120 42.079 202 111 4 142 341 66 263 3.57e-36 132
MsG0680035812.01.T01 MTR_7g017150 42.784 194 107 3 148 341 75 264 4.71e-36 132
MsG0680035812.01.T01 MTR_8g035690 34.256 289 146 11 77 340 30 299 9.87e-34 126
MsG0680035812.01.T01 MTR_5g096920 44.805 154 76 4 186 338 1 146 2.29e-32 118
MsG0680035812.01.T01 MTR_5g097660 52.101 119 54 2 225 342 2 118 8.98e-32 115
MsG0680035812.01.T01 MTR_5g096900 34.926 272 128 10 13 265 72 313 2.91e-31 120
MsG0680035812.01.T01 MTR_8g064790 30.696 316 141 15 29 339 90 332 2.52e-29 115
MsG0680035812.01.T01 MTR_5g036250 52.427 103 46 1 238 340 8 107 4.37e-29 110
MsG0680035812.01.T01 MTR_5g097540 45.205 146 65 5 155 297 97 230 2.34e-28 112
MsG0680035812.01.T01 MTR_2g104260 35.354 198 109 5 30 218 90 277 1.06e-26 107
MsG0680035812.01.T01 MTR_5g086780 53.465 101 47 0 218 318 128 228 6.84e-26 103
MsG0680035812.01.T01 MTR_2g022020 43.220 118 63 1 221 338 1 114 2.18e-24 96.3
MsG0680035812.01.T01 MTR_5g094350 34.946 186 100 5 54 227 100 276 2.49e-23 97.8
MsG0680035812.01.T01 MTR_6g465360 40.690 145 69 6 31 163 142 281 8.16e-22 94.0
MsG0680035812.01.T01 MTR_5g097820 34.946 186 85 8 29 202 102 263 8.51e-22 93.6
MsG0680035812.01.T01 MTR_7g101200 30.417 240 121 10 63 298 30 227 1.78e-21 92.0
MsG0680035812.01.T01 MTR_5g091150 34.615 208 74 8 53 255 44 194 1.73e-19 85.5
MsG0680035812.01.T01 MTR_1g076810 34.118 170 91 6 22 187 89 241 4.63e-19 88.6
MsG0680035812.01.T01 MTR_5g096050 45.370 108 50 1 186 284 1 108 1.88e-18 80.5
MsG0680035812.01.T01 MTR_4g133170 22.711 273 170 9 51 312 88 330 6.47e-17 81.3
MsG0680035812.01.T01 MTR_1g115205 31.844 179 78 8 29 194 90 237 8.37e-17 79.3
MsG0680035812.01.T01 MTR_1g050308 32.544 169 81 9 11 172 94 236 3.32e-16 79.0
MsG0680035812.01.T01 MTR_1g050308 41.818 110 55 3 234 339 238 342 1.13e-15 77.4
MsG0680035812.01.T01 MTR_1g047080 26.814 317 155 14 29 340 35 279 2.89e-15 75.5
MsG0680035812.01.T01 MTR_2g024100 22.183 284 169 9 56 315 106 361 8.43e-15 75.1
MsG0680035812.01.T01 MTR_5g037190 33.880 183 85 6 20 199 86 235 1.59e-14 72.8
MsG0680035812.01.T01 MTR_2g028130 22.642 265 164 8 67 314 125 365 2.88e-14 73.6
MsG0680035812.01.T01 MTR_3g013740 25.161 310 181 13 4 304 99 366 5.86e-14 72.8
MsG0680035812.01.T01 MTR_1g050305 48.810 84 38 2 243 321 21 104 9.99e-14 68.2
MsG0680035812.01.T01 MTR_8g101580 27.391 230 131 10 49 277 136 330 7.58e-13 69.3
MsG0680035812.01.T01 MTR_6g065980 24.000 300 171 11 25 324 89 331 7.62e-13 68.9
MsG0680035812.01.T01 MTR_2g024110 23.397 312 174 14 36 314 63 342 1.15e-12 68.6
MsG0680035812.01.T01 MTR_3g083010 22.857 280 171 9 56 315 118 372 2.23e-12 68.2
MsG0680035812.01.T01 MTR_2g426090 26.103 272 154 14 51 303 98 341 5.46e-12 66.6
MsG0680035812.01.T01 MTR_2g031640 22.909 275 171 11 51 312 90 336 7.29e-12 66.2
MsG0680035812.01.T01 MTR_1g068860 21.250 320 211 11 34 342 75 364 8.04e-12 66.2
MsG0680035812.01.T01 MTR_2g031720 23.656 279 170 12 51 315 94 343 9.76e-12 65.9
MsG0680035812.01.T01 MTR_3g024130 24.727 275 164 12 33 304 40 274 1.04e-11 65.5
MsG0680035812.01.T01 MTR_2g025960 22.148 298 169 15 51 317 3 268 1.21e-11 65.1
MsG0680035812.01.T01 MTR_3g009310 25.630 238 135 10 42 278 131 327 1.37e-11 65.5
MsG0680035812.01.T01 MTR_4g061340 27.559 254 151 12 31 281 96 319 1.59e-11 65.1
MsG0680035812.01.T01 MTR_2g028110 23.050 282 168 12 51 315 88 337 1.80e-11 65.1
MsG0680035812.01.T01 MTR_3g058480 26.582 158 86 4 47 202 137 266 2.13e-11 64.7
MsG0680035812.01.T01 MTR_5g070080 23.510 302 188 11 42 338 113 376 3.11e-11 64.3
MsG0680035812.01.T01 MTR_2g021050 21.986 282 161 10 50 315 92 330 4.36e-11 63.9
MsG0680035812.01.T01 MTR_2g425980 22.840 324 192 14 41 339 86 376 5.09e-11 63.9
MsG0680035812.01.T01 MTR_2g021150 24.756 307 180 14 4 300 67 332 5.51e-11 63.5
MsG0680035812.01.T01 MTR_3g097190 44.118 68 34 1 262 329 28 91 5.87e-11 61.2
MsG0680035812.01.T01 MTR_8g102520 25.984 254 147 13 74 319 75 295 6.62e-11 63.2
MsG0680035812.01.T01 MTR_6g060650 24.573 293 172 13 54 338 113 364 6.88e-11 63.2
MsG0680035812.01.T01 MTR_3g058440 27.568 185 99 8 42 224 126 277 7.44e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 74 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGAAGAAGTTTGATTATT+TGG 0.100222 6:-112659098 None:intergenic
AAATAAAGATACTCTTTAAA+TGG 0.198028 6:-112658985 None:intergenic
AGGCTATATATCTCCCATTT+TGG 0.222547 6:-112659318 None:intergenic
CTCTTCTCATCTTGAATTGA+TGG 0.274458 6:+112658664 MsG0680035812.01.T01:CDS
CCGTCTCGTCTTCATGGATT+TGG 0.279470 6:+112659162 MsG0680035812.01.T01:CDS
TATGTTCCGTCTCGTCTTCA+TGG 0.283062 6:+112659156 MsG0680035812.01.T01:CDS
TTGTAGGATAATAAATTATA+AGG 0.289217 6:+112659842 MsG0680035812.01.T01:CDS
TCAAAGAATTTGTTTCTTAC+CGG 0.308819 6:+112659274 MsG0680035812.01.T01:CDS
TGGAAGAAGTTTGATTATTT+GGG 0.315174 6:-112659097 None:intergenic
AGAGTGCTCATTCTCTTTGT+AGG 0.333373 6:+112659826 MsG0680035812.01.T01:CDS
ACACACCGTCCATGTAGATT+TGG 0.338077 6:-112659407 None:intergenic
ATATTAATTTAGTGCCAAAA+TGG 0.343855 6:+112659304 MsG0680035812.01.T01:CDS
ATCGTGGATCAAACTCTTAA+TGG 0.345702 6:+112659668 MsG0680035812.01.T01:CDS
ATAAACTTGGTGGTGTTAAA+TGG 0.359513 6:+112659567 MsG0680035812.01.T01:CDS
AGTTTGATTATTTGGGTAGT+TGG 0.371724 6:-112659090 None:intergenic
CAAACTTCTTCCACGTAGTA+AGG 0.376289 6:+112659107 MsG0680035812.01.T01:CDS
TAATCCCGTTGGACCATGTT+TGG 0.378580 6:+112659458 MsG0680035812.01.T01:CDS
TAAACTACTTATTCCTCAAA+TGG 0.380948 6:-112659876 None:intergenic
GTTTGATTATTTGGGTAGTT+GGG 0.385461 6:-112659089 None:intergenic
GGAAAGCATTGTTCCATTTG+AGG 0.409137 6:+112659863 MsG0680035812.01.T01:CDS
TCAAAACAATCATCTGCGTC+TGG 0.412061 6:-112659528 None:intergenic
CCTATCGGAGTGGGGACAAA+GGG 0.426606 6:+112659717 MsG0680035812.01.T01:CDS
CACACCGTCCATGTAGATTT+GGG 0.428429 6:-112659406 None:intergenic
GATACCAAACATGGTCCAAC+GGG 0.436633 6:-112659462 None:intergenic
CATGTAGATTTGGGTGCCTT+CGG 0.439153 6:-112659397 None:intergenic
CCCTTTGTCCCCACTCCGAT+AGG 0.439470 6:-112659717 None:intergenic
GATTTGGGTGCCTTCGGTAC+AGG 0.444606 6:-112659391 None:intergenic
TATTAATTTAGTGCCAAAAT+GGG 0.485477 6:+112659305 MsG0680035812.01.T01:CDS
CCCTATCGGAGTGGGGACAA+AGG 0.498257 6:+112659716 MsG0680035812.01.T01:CDS
CAAAGAATTTGTTTCTTACC+GGG 0.499031 6:+112659275 MsG0680035812.01.T01:CDS
ACTCAAATGATTGAGTTGCT+TGG 0.499407 6:+112659792 MsG0680035812.01.T01:CDS
TAAAAGATAATGAAGTGGCT+TGG 0.502515 6:+112659757 MsG0680035812.01.T01:CDS
GAATTTGTTTCTTACCGGGT+TGG 0.503130 6:+112659279 MsG0680035812.01.T01:CDS
CAAAACAATCATCTGCGTCT+GGG 0.503412 6:-112659527 None:intergenic
CCTTACGTTGAGCGCCCTAT+CGG 0.508965 6:+112659702 MsG0680035812.01.T01:CDS
CATTATAGTCATTCGTAACA+TGG 0.509529 6:-112659191 None:intergenic
ACATGGACGGTGTGTGTCAT+TGG 0.510290 6:+112659415 MsG0680035812.01.T01:CDS
CCGATAGGGCGCTCAACGTA+AGG 0.515943 6:-112659702 None:intergenic
ATGATATGAGATCAATGCTA+TGG 0.518105 6:-112659592 None:intergenic
CCTTTGTCCCCACTCCGATA+GGG 0.536905 6:-112659716 None:intergenic
CGAAGGCACCCAAATCTACA+TGG 0.538690 6:+112659398 MsG0680035812.01.T01:CDS
TCTTCTGAGGCCTGTACCGA+AGG 0.541197 6:+112659381 MsG0680035812.01.T01:CDS
GTTGAGCGCCCTATCGGAGT+GGG 0.544996 6:+112659708 MsG0680035812.01.T01:CDS
TGGATCAAACTCTTAATGGT+TGG 0.555769 6:+112659672 MsG0680035812.01.T01:CDS
AATGGGAGATATATAGCCTA+AGG 0.561188 6:+112659322 MsG0680035812.01.T01:CDS
GGTTGCTGCGACGAACGAAA+AGG 0.564400 6:+112658703 MsG0680035812.01.T01:CDS
AAAACAATCATCTGCGTCTG+GGG 0.565772 6:-112659526 None:intergenic
AAACCATCATATGATTACTC+TGG 0.567062 6:+112659246 MsG0680035812.01.T01:CDS
TCGGTACAGGCCTCAGAAGA+CGG 0.568106 6:-112659378 None:intergenic
TCACCAGAGTAATCATATGA+TGG 0.568791 6:-112659249 None:intergenic
AAGCAATGAACAATAATCAC+AGG 0.569158 6:+112658633 None:intergenic
AAACATAAAGATAATCCCGT+TGG 0.570917 6:+112659447 MsG0680035812.01.T01:CDS
ATTATCGCAAAATTGTATTG+TGG 0.571300 6:+112659064 MsG0680035812.01.T01:CDS
TGTTGACATGCCGTCTTCTG+AGG 0.572263 6:+112659368 MsG0680035812.01.T01:CDS
CATAATAAAAGATAATGAAG+TGG 0.576619 6:+112659752 MsG0680035812.01.T01:CDS
TCCACGTAGTAAGGTTGAGT+CGG 0.581495 6:+112659116 MsG0680035812.01.T01:CDS
TAAAGCAAAGTGGATAAACT+TGG 0.582061 6:+112659554 MsG0680035812.01.T01:CDS
TGATACCAAACATGGTCCAA+CGG 0.596717 6:-112659463 None:intergenic
AGCAAAGTGGATAAACTTGG+TGG 0.604972 6:+112659557 MsG0680035812.01.T01:CDS
CGTTGAGCGCCCTATCGGAG+TGG 0.607842 6:+112659707 MsG0680035812.01.T01:CDS
AACTCGGTTTCAAAGAATCG+TGG 0.617879 6:+112659652 MsG0680035812.01.T01:CDS
GGCACCCAAATCTACATGGA+CGG 0.622775 6:+112659402 MsG0680035812.01.T01:CDS
CTAAATTAATATCTCCAACC+CGG 0.631478 6:-112659293 None:intergenic
ATAGCCTAAGGAGTAACTCG+TGG 0.639939 6:+112659334 MsG0680035812.01.T01:CDS
CTCTCCACGAGTTACTCCTT+AGG 0.643218 6:-112659338 None:intergenic
GGGCGCTCAACGTAAGGCAA+CGG 0.644355 6:-112659696 None:intergenic
TATCGGAGTGGGGACAAAGG+GGG 0.649198 6:+112659719 MsG0680035812.01.T01:CDS
AAACAATCATCTGCGTCTGG+GGG 0.653926 6:-112659525 None:intergenic
AAGTAAAATGATACCAAACA+TGG 0.663221 6:-112659471 None:intergenic
CCAAATCCATGAAGACGAGA+CGG 0.664386 6:-112659162 None:intergenic
CTATCGGAGTGGGGACAAAG+GGG 0.669039 6:+112659718 MsG0680035812.01.T01:CDS
GATGGAGAAGAACAAATCAG+TGG 0.670747 6:+112658682 MsG0680035812.01.T01:CDS
TTGAGCGCCCTATCGGAGTG+GGG 0.675422 6:+112659709 MsG0680035812.01.T01:CDS
TCCGACTCAACCTTACTACG+TGG 0.745267 6:-112659117 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAGATACTCTTTAAA+TGG - Chr6:112658988-112659007 None:intergenic 15.0%
!! TTGTAGGATAATAAATTATA+AGG + Chr6:112659842-112659861 MsG0680035812.01.T01:CDS 15.0%
!!! AATTATTTTGTTCATTTTGA+TGG - Chr6:112659047-112659066 None:intergenic 15.0%
!!! GATATTTTTACTAGTATTAA+TGG + Chr6:112659009-112659028 MsG0680035812.01.T01:CDS 15.0%
!! ATATTAATTTAGTGCCAAAA+TGG + Chr6:112659304-112659323 MsG0680035812.01.T01:CDS 20.0%
!! CATAATAAAAGATAATGAAG+TGG + Chr6:112659752-112659771 MsG0680035812.01.T01:CDS 20.0%
!! TATTAATTTAGTGCCAAAAT+GGG + Chr6:112659305-112659324 MsG0680035812.01.T01:CDS 20.0%
!!! ACTTTTCATGTATCAATTTT+GGG + Chr6:112659627-112659646 MsG0680035812.01.T01:CDS 20.0%
!!! TACTTTTCATGTATCAATTT+TGG + Chr6:112659626-112659645 MsG0680035812.01.T01:CDS 20.0%
! AAGTAAAATGATACCAAACA+TGG - Chr6:112659474-112659493 None:intergenic 25.0%
! AATATCATCAGATATGTATG+CGG - Chr6:112658735-112658754 None:intergenic 25.0%
! ATTATCGCAAAATTGTATTG+TGG + Chr6:112659064-112659083 MsG0680035812.01.T01:CDS 25.0%
! TGGAAGAAGTTTGATTATTT+GGG - Chr6:112659100-112659119 None:intergenic 25.0%
!! AGTTTTCATTATCAAAGAGT+AGG - Chr6:112658897-112658916 None:intergenic 25.0%
!! TCAAAGAATTTGTTTCTTAC+CGG + Chr6:112659274-112659293 MsG0680035812.01.T01:CDS 25.0%
AAACATAAAGATAATCCCGT+TGG + Chr6:112659447-112659466 MsG0680035812.01.T01:CDS 30.0%
AAACCATCATATGATTACTC+TGG + Chr6:112659246-112659265 MsG0680035812.01.T01:CDS 30.0%
AGTTTGATTATTTGGGTAGT+TGG - Chr6:112659093-112659112 None:intergenic 30.0%
ATGATATGAGATCAATGCTA+TGG - Chr6:112659595-112659614 None:intergenic 30.0%
CATTATAGTCATTCGTAACA+TGG - Chr6:112659194-112659213 None:intergenic 30.0%
CTAAATTAATATCTCCAACC+CGG - Chr6:112659296-112659315 None:intergenic 30.0%
GTGGAAGAAGTTTGATTATT+TGG - Chr6:112659101-112659120 None:intergenic 30.0%
GTTTGATTATTTGGGTAGTT+GGG - Chr6:112659092-112659111 None:intergenic 30.0%
TAAAAGATAATGAAGTGGCT+TGG + Chr6:112659757-112659776 MsG0680035812.01.T01:CDS 30.0%
TAAAGCAAAGTGGATAAACT+TGG + Chr6:112659554-112659573 MsG0680035812.01.T01:CDS 30.0%
! CAAAGAATTTGTTTCTTACC+GGG + Chr6:112659275-112659294 MsG0680035812.01.T01:CDS 30.0%
!! AAGAGGTAATTTTGAGAGAA+TGG - Chr6:112658765-112658784 None:intergenic 30.0%
!! ATAAACTTGGTGGTGTTAAA+TGG + Chr6:112659567-112659586 MsG0680035812.01.T01:CDS 30.0%
!! GAAGTTTTGTATTCTGTTTC+TGG + Chr6:112658922-112658941 MsG0680035812.01.T01:intron 30.0%
!! GTTTTGATGTTAAAGCAAAG+TGG + Chr6:112659544-112659563 MsG0680035812.01.T01:CDS 30.0%
!! TAATTTTGAGAGAATGGAGA+AGG - Chr6:112658759-112658778 None:intergenic 30.0%
AAACGCTTGAAAGACTTAAG+AGG - Chr6:112658782-112658801 None:intergenic 35.0%
AATGGGAGATATATAGCCTA+AGG + Chr6:112659322-112659341 MsG0680035812.01.T01:CDS 35.0%
ATCGTGGATCAAACTCTTAA+TGG + Chr6:112659668-112659687 MsG0680035812.01.T01:CDS 35.0%
CTCTTCTCATCTTGAATTGA+TGG + Chr6:112658664-112658683 MsG0680035812.01.T01:CDS 35.0%
TCACCAGAGTAATCATATGA+TGG - Chr6:112659252-112659271 None:intergenic 35.0%
TGGATCAAACTCTTAATGGT+TGG + Chr6:112659672-112659691 MsG0680035812.01.T01:CDS 35.0%
! ACTCAAATGATTGAGTTGCT+TGG + Chr6:112659792-112659811 MsG0680035812.01.T01:CDS 35.0%
! AGGCTATATATCTCCCATTT+TGG - Chr6:112659321-112659340 None:intergenic 35.0%
!! GTATCAATTTTGGGTCAACT+CGG + Chr6:112659636-112659655 MsG0680035812.01.T01:CDS 35.0%
AAAACAATCATCTGCGTCTG+GGG - Chr6:112659529-112659548 None:intergenic 40.0%
AACTCGGTTTCAAAGAATCG+TGG + Chr6:112659652-112659671 MsG0680035812.01.T01:CDS 40.0%
AGCAAAGTGGATAAACTTGG+TGG + Chr6:112659557-112659576 MsG0680035812.01.T01:CDS 40.0%
CAAAACAATCATCTGCGTCT+GGG - Chr6:112659530-112659549 None:intergenic 40.0%
CAAACTTCTTCCACGTAGTA+AGG + Chr6:112659107-112659126 MsG0680035812.01.T01:CDS 40.0%
GATGGAGAAGAACAAATCAG+TGG + Chr6:112658682-112658701 MsG0680035812.01.T01:CDS 40.0%
GCTTCACATTGAGATTGTAG+CGG - Chr6:112658865-112658884 None:intergenic 40.0%
TCAAAACAATCATCTGCGTC+TGG - Chr6:112659531-112659550 None:intergenic 40.0%
TGATACCAAACATGGTCCAA+CGG - Chr6:112659466-112659485 None:intergenic 40.0%
! AGAGTGCTCATTCTCTTTGT+AGG + Chr6:112659826-112659845 MsG0680035812.01.T01:CDS 40.0%
! GAATTTGTTTCTTACCGGGT+TGG + Chr6:112659279-112659298 MsG0680035812.01.T01:CDS 40.0%
!! GGAAAGCATTGTTCCATTTG+AGG + Chr6:112659863-112659882 MsG0680035812.01.T01:CDS 40.0%
!! TGTATTCTGTTTCTGGTGAG+AGG + Chr6:112658929-112658948 MsG0680035812.01.T01:intron 40.0%
AAACAATCATCTGCGTCTGG+GGG - Chr6:112659528-112659547 None:intergenic 45.0%
ACACACCGTCCATGTAGATT+TGG - Chr6:112659410-112659429 None:intergenic 45.0%
ATAGCCTAAGGAGTAACTCG+TGG + Chr6:112659334-112659353 MsG0680035812.01.T01:CDS 45.0%
CACACCGTCCATGTAGATTT+GGG - Chr6:112659409-112659428 None:intergenic 45.0%
CATGTAGATTTGGGTGCCTT+CGG - Chr6:112659400-112659419 None:intergenic 45.0%
CCAAATCCATGAAGACGAGA+CGG - Chr6:112659165-112659184 None:intergenic 45.0%
GATACCAAACATGGTCCAAC+GGG - Chr6:112659465-112659484 None:intergenic 45.0%
TAATCCCGTTGGACCATGTT+TGG + Chr6:112659458-112659477 MsG0680035812.01.T01:CDS 45.0%
TATGTTCCGTCTCGTCTTCA+TGG + Chr6:112659156-112659175 MsG0680035812.01.T01:CDS 45.0%
TCCACGTAGTAAGGTTGAGT+CGG + Chr6:112659116-112659135 MsG0680035812.01.T01:CDS 45.0%
ACATGGACGGTGTGTGTCAT+TGG + Chr6:112659415-112659434 MsG0680035812.01.T01:CDS 50.0%
CCGTCTCGTCTTCATGGATT+TGG + Chr6:112659162-112659181 MsG0680035812.01.T01:CDS 50.0%
CTCTCCACGAGTTACTCCTT+AGG - Chr6:112659341-112659360 None:intergenic 50.0%
GGCACCCAAATCTACATGGA+CGG + Chr6:112659402-112659421 MsG0680035812.01.T01:CDS 50.0%
TCCGACTCAACCTTACTACG+TGG - Chr6:112659120-112659139 None:intergenic 50.0%
TGTTGACATGCCGTCTTCTG+AGG + Chr6:112659368-112659387 MsG0680035812.01.T01:CDS 50.0%
!! CGAAGGCACCCAAATCTACA+TGG + Chr6:112659398-112659417 MsG0680035812.01.T01:CDS 50.0%
ATCATCTGCGTCTGGGGGTA+TGG - Chr6:112659523-112659542 None:intergenic 55.0%
CCTATCGGAGTGGGGACAAA+GGG + Chr6:112659717-112659736 MsG0680035812.01.T01:CDS 55.0%
CCTTACGTTGAGCGCCCTAT+CGG + Chr6:112659702-112659721 MsG0680035812.01.T01:CDS 55.0%
CCTTTGTCCCCACTCCGATA+GGG - Chr6:112659719-112659738 None:intergenic 55.0%
CTATCGGAGTGGGGACAAAG+GGG + Chr6:112659718-112659737 MsG0680035812.01.T01:CDS 55.0%
GATTTGGGTGCCTTCGGTAC+AGG - Chr6:112659394-112659413 None:intergenic 55.0%
GGTTGCTGCGACGAACGAAA+AGG + Chr6:112658703-112658722 MsG0680035812.01.T01:CDS 55.0%
TATCGGAGTGGGGACAAAGG+GGG + Chr6:112659719-112659738 MsG0680035812.01.T01:CDS 55.0%
TCGGTACAGGCCTCAGAAGA+CGG - Chr6:112659381-112659400 None:intergenic 55.0%
! TGGGGGTATGGCTGTTGTGA+AGG - Chr6:112659511-112659530 None:intergenic 55.0%
!! TCTTCTGAGGCCTGTACCGA+AGG + Chr6:112659381-112659400 MsG0680035812.01.T01:CDS 55.0%
CCCTATCGGAGTGGGGACAA+AGG + Chr6:112659716-112659735 MsG0680035812.01.T01:CDS 60.0%
CCCTTTGTCCCCACTCCGAT+AGG - Chr6:112659720-112659739 None:intergenic 60.0%
GGGCGCTCAACGTAAGGCAA+CGG - Chr6:112659699-112659718 None:intergenic 60.0%
GTTGAGCGCCCTATCGGAGT+GGG + Chr6:112659708-112659727 MsG0680035812.01.T01:CDS 60.0%
TTGAGCGCCCTATCGGAGTG+GGG + Chr6:112659709-112659728 MsG0680035812.01.T01:CDS 60.0%
! CCGATAGGGCGCTCAACGTA+AGG - Chr6:112659705-112659724 None:intergenic 60.0%
CGTTGAGCGCCCTATCGGAG+TGG + Chr6:112659707-112659726 MsG0680035812.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr6 gene 112658638 112659890 112658638 ID=MsG0680035812.01;Name=MsG0680035812.01
Chr6 mRNA 112658638 112659890 112658638 ID=MsG0680035812.01.T01;Parent=MsG0680035812.01;Name=MsG0680035812.01.T01;_AED=0.42;_eAED=0.42;_QI=0|0|0|1|1|1|2|0|342
Chr6 exon 112658638 112658724 112658638 ID=MsG0680035812.01.T01:exon:9641;Parent=MsG0680035812.01.T01
Chr6 exon 112658949 112659890 112658949 ID=MsG0680035812.01.T01:exon:9642;Parent=MsG0680035812.01.T01
Chr6 CDS 112658638 112658724 112658638 ID=MsG0680035812.01.T01:cds;Parent=MsG0680035812.01.T01
Chr6 CDS 112658949 112659890 112658949 ID=MsG0680035812.01.T01:cds;Parent=MsG0680035812.01.T01
Gene Sequence

>MsG0680035812.01.T01

ATGAACAATAATCACAGGCGAACAAGCTCTTCTCATCTTGAATTGATGGAGAAGAACAAATCAGTGGTTGCTGCGACGAACGAAAAGAGGTTCAAGAATAAAATCAAATTAGATTTTTCAAATCCATTTAAAGAGTATCTTTATTTTGATATTTTTACTAGTATTAATGGTACAATTTGTCTCCATCAAAATGAACAAAATAATTATCGCAAAATTGTATTGTGGAACCCAACTACCCAAATAATCAAACTTCTTCCACGTAGTAAGGTTGAGTCGGATAGTTTTTTTGATATTTATGTTCCGTCTCGTCTTCATGGATTTGGTTATAACCATGTTACGAATGACTATAATGTCATTCAACTTATAAAAGTTTGTATAAAAGAAAAACCATCATATGATTACTCTGGTGACTTCAAAGAATTTGTTTCTTACCGGGTTGGAGATATTAATTTAGTGCCAAAATGGGAGATATATAGCCTAAGGAGTAACTCGTGGAGAGAACTTGATGTTGACATGCCGTCTTCTGAGGCCTGTACCGAAGGCACCCAAATCTACATGGACGGTGTGTGTCATTGGTTGTGTGAAAAACATAAAGATAATCCCGTTGGACCATGTTTGGTATCATTTTACTTGAGCAATGAAGTGTCCTTCACAACAGCCATACCCCCAGACGCAGATGATTGTTTTGATGTTAAAGCAAAGTGGATAAACTTGGTGGTGTTAAATGGATCCATAGCATTGATCTCATATCATGTAAAGACGACTACTTTTCATGTATCAATTTTGGGTCAACTCGGTTTCAAAGAATCGTGGATCAAACTCTTAATGGTTGGACCGTTGCCTTACGTTGAGCGCCCTATCGGAGTGGGGACAAAGGGGGAAATATTCTTCATAATAAAAGATAATGAAGTGGCTTGGTTTGATTTGAATACTCAAATGATTGAGTTGCTTGGTTATACAACAGAGAGTGCTCATTCTCTTTGTAGGATAATAAATTATAAGGAAAGCATTGTTCCATTTGAGGAATAA
Protein sequence

>MsG0680035812.01.T01

MNNNHRRTSSSHLELMEKNKSVVAATNEKRFKNKIKLDFSNPFKEYLYFDIFTSINGTICLHQNEQNNYRKIVLWNPTTQIIKLLPRSKVESDSFFDIYVPSRLHGFGYNHVTNDYNVIQLIKVCIKEKPSYDYSGDFKEFVSYRVGDINLVPKWEIYSLRSNSWRELDVDMPSSEACTEGTQIYMDGVCHWLCEKHKDNPVGPCLVSFYLSNEVSFTTAIPPDADDCFDVKAKWINLVVLNGSIALISYHVKTTTFHVSILGQLGFKESWIKLLMVGPLPYVERPIGVGTKGEIFFIIKDNEVAWFDLNTQMIELLGYTTESAHSLCRIINYKESIVPFEE*