AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001502.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001502.01.T01 MTR_5g096900 48.997 349 125 14 9 351 6 307 1.01e-83 258
MsG0180001502.01.T01 MTR_5g086590 70.930 172 49 1 181 351 5 176 3.78e-83 253
MsG0180001502.01.T01 MTR_5g037680 43.931 346 142 7 11 351 1 299 1.49e-77 246
MsG0180001502.01.T01 MTR_5g096910 42.541 362 138 10 3 351 4 308 2.48e-73 233
MsG0180001502.01.T01 MTR_2g022000 40.164 366 146 8 15 351 13 334 6.60e-70 226
MsG0180001502.01.T01 MTR_7g017130 37.088 364 166 7 1 351 1 314 3.98e-69 223
MsG0180001502.01.T01 MTR_7g017170 37.088 364 166 7 1 351 1 314 4.43e-69 223
MsG0180001502.01.T01 MTR_0129s0070 39.118 363 152 10 5 351 4 313 2.38e-67 218
MsG0180001502.01.T01 MTR_8g012090 39.945 363 120 10 3 351 4 282 3.47e-67 216
MsG0180001502.01.T01 MTR_5g069390 38.965 367 165 10 2 351 10 334 2.06e-66 218
MsG0180001502.01.T01 MTR_2g021800 38.420 367 152 8 15 351 13 335 1.88e-64 211
MsG0180001502.01.T01 MTR_7g017040 39.888 356 142 7 16 352 14 316 2.25e-64 210
MsG0180001502.01.T01 MTR_2g038680 39.583 384 135 13 4 351 5 327 3.18e-64 210
MsG0180001502.01.T01 MTR_5g059430 38.338 373 167 10 2 351 10 342 3.52e-64 211
MsG0180001502.01.T01 MTR_5g097820 43.234 303 125 7 4 302 10 269 6.97e-64 205
MsG0180001502.01.T01 MTR_7g069550 36.565 361 151 10 15 351 11 317 1.16e-63 208
MsG0180001502.01.T01 MTR_5g024260 37.203 379 169 11 1 364 5 329 6.86e-63 207
MsG0180001502.01.T01 MTR_5g097330 39.316 351 155 12 15 351 13 319 6.89e-63 206
MsG0180001502.01.T01 MTR_0129s0120 38.292 363 155 10 5 351 4 313 8.36e-63 206
MsG0180001502.01.T01 MTR_4g055820 37.634 372 153 10 6 351 2 320 1.28e-60 199
MsG0180001502.01.T01 MTR_5g096630 39.326 356 139 9 17 348 17 319 3.26e-60 200
MsG0180001502.01.T01 MTR_4g102410 38.082 365 139 10 15 350 58 364 1.18e-59 199
MsG0180001502.01.T01 MTR_7g016930 38.617 347 134 7 15 351 13 290 1.64e-58 193
MsG0180001502.01.T01 MTR_3g011120 37.784 352 153 12 8 351 4 297 1.76e-58 194
MsG0180001502.01.T01 MTR_8g063440 39.143 350 142 13 15 351 9 300 2.24e-58 194
MsG0180001502.01.T01 MTR_1g007410 37.057 367 151 9 5 347 9 319 6.74e-58 194
MsG0180001502.01.T01 MTR_2g034700 38.166 338 143 9 14 346 10 286 3.97e-56 188
MsG0180001502.01.T01 MTR_0129s0050 36.631 374 142 10 15 351 11 326 6.47e-56 189
MsG0180001502.01.T01 MTR_6g086825 38.108 370 139 13 14 351 66 377 9.47e-56 189
MsG0180001502.01.T01 MTR_4g117550 40.181 331 128 11 9 322 8 285 2.17e-55 186
MsG0180001502.01.T01 MTR_5g097350 37.725 334 147 10 27 351 25 306 3.50e-55 186
MsG0180001502.01.T01 MTR_6g080680 37.190 363 136 11 15 351 16 312 4.38e-55 186
MsG0180001502.01.T01 MTR_5g097680 36.719 384 146 11 15 351 14 347 2.27e-54 185
MsG0180001502.01.T01 MTR_8g464770 38.209 335 147 9 15 343 9 289 8.42e-54 182
MsG0180001502.01.T01 MTR_8g039500 36.550 342 147 10 15 348 9 288 1.38e-53 181
MsG0180001502.01.T01 MTR_1g007450 38.764 356 146 14 16 351 10 313 2.10e-53 182
MsG0180001502.01.T01 MTR_2g021950 37.079 356 151 12 16 351 14 316 8.66e-53 180
MsG0180001502.01.T01 MTR_6g461660 37.432 366 155 12 6 351 7 318 1.06e-52 180
MsG0180001502.01.T01 MTR_4g028680 37.265 373 156 13 1 351 1 317 2.14e-52 179
MsG0180001502.01.T01 MTR_2g065860 34.758 351 155 9 17 351 11 303 2.74e-52 178
MsG0180001502.01.T01 MTR_8g041270 36.944 360 152 11 16 350 13 322 1.33e-51 177
MsG0180001502.01.T01 MTR_0054s0090 36.267 375 152 13 2 348 5 320 1.41e-51 177
MsG0180001502.01.T01 MTR_5g075370 37.795 381 128 18 13 351 3 316 3.65e-51 182
MsG0180001502.01.T01 MTR_4g028660 34.584 373 165 12 1 351 1 316 7.98e-51 175
MsG0180001502.01.T01 MTR_4g027450 34.584 373 165 12 1 351 1 316 7.98e-51 175
MsG0180001502.01.T01 MTR_0054s0100 35.450 378 159 13 2 351 5 325 1.35e-50 174
MsG0180001502.01.T01 MTR_4g028750 36.389 360 153 14 13 351 11 315 1.75e-50 174
MsG0180001502.01.T01 MTR_2g066200 35.072 345 157 9 15 347 9 298 2.25e-50 173
MsG0180001502.01.T01 MTR_8g013000 34.844 353 158 10 16 348 8 308 2.28e-50 174
MsG0180001502.01.T01 MTR_8g046310 37.394 353 156 13 4 351 2 294 2.74e-50 173
MsG0180001502.01.T01 MTR_1g053510 35.958 381 156 12 1 351 1 323 3.09e-50 174
MsG0180001502.01.T01 MTR_2g021770 35.753 372 148 11 15 351 9 324 3.15e-50 174
MsG0180001502.01.T01 MTR_5g074850 39.305 374 119 20 18 351 35 340 3.36e-50 181
MsG0180001502.01.T01 MTR_6g090180 35.635 362 158 11 15 351 44 355 3.84e-50 174
MsG0180001502.01.T01 MTR_8g033140 33.419 389 162 11 2 351 4 334 5.33e-50 173
MsG0180001502.01.T01 MTR_5g072060 33.699 365 136 9 15 351 3 289 1.73e-49 176
MsG0180001502.01.T01 MTR_2g066170 34.884 344 157 9 15 347 9 296 2.69e-49 171
MsG0180001502.01.T01 MTR_2g104280 35.519 366 152 14 16 351 12 323 4.78e-49 170
MsG0180001502.01.T01 MTR_7g079640 35.260 346 147 10 17 351 12 291 4.80e-49 170
MsG0180001502.01.T01 MTR_4g130330 36.364 363 148 13 14 351 3 307 2.04e-48 168
MsG0180001502.01.T01 MTR_4g028510 34.667 375 165 14 1 351 1 319 2.62e-48 170
MsG0180001502.01.T01 MTR_5g097220 34.974 386 145 12 6 351 4 323 5.23e-48 168
MsG0180001502.01.T01 MTR_2g064950 34.066 364 155 10 9 348 1 303 8.12e-48 167
MsG0180001502.01.T01 MTR_5g091140 36.620 355 136 10 17 351 47 332 1.25e-47 167
MsG0180001502.01.T01 MTR_6g079810 36.968 376 152 12 7 351 59 380 1.32e-47 168
MsG0180001502.01.T01 MTR_7g111700 36.565 361 122 13 12 351 9 283 1.56e-47 165
MsG0180001502.01.T01 MTR_0831s0010 34.349 361 167 12 4 352 37 339 2.34e-47 166
MsG0180001502.01.T01 MTR_2g065840 33.146 356 157 9 22 358 16 309 2.72e-47 165
MsG0180001502.01.T01 MTR_8g063330 33.967 368 150 13 13 352 9 311 6.37e-47 166
MsG0180001502.01.T01 MTR_5g092850 33.766 385 148 15 2 351 4 316 1.01e-46 164
MsG0180001502.01.T01 MTR_0134s0060 33.246 382 162 12 10 351 1 329 1.05e-46 164
MsG0180001502.01.T01 MTR_8g032980 34.109 387 148 12 13 351 11 338 1.16e-46 164
MsG0180001502.01.T01 MTR_8g033110 32.570 393 162 11 1 351 1 332 4.22e-46 163
MsG0180001502.01.T01 MTR_8g467670 35.569 343 151 13 14 347 10 291 7.02e-46 162
MsG0180001502.01.T01 MTR_2g104340 35.342 365 145 12 16 351 12 314 1.94e-45 160
MsG0180001502.01.T01 MTR_7g104710 33.705 359 143 12 7 347 3 284 1.09e-44 157
MsG0180001502.01.T01 MTR_6g079770 33.245 376 155 12 16 351 15 334 1.83e-44 159
MsG0180001502.01.T01 MTR_1g007430 35.072 345 134 12 29 351 7 283 4.17e-44 155
MsG0180001502.01.T01 MTR_2g066150 34.808 339 135 13 17 348 11 270 6.42e-44 155
MsG0180001502.01.T01 MTR_4g014370 35.799 338 125 13 15 312 8 293 2.66e-43 155
MsG0180001502.01.T01 MTR_5g037190 35.065 308 132 6 6 305 4 251 2.97e-43 151
MsG0180001502.01.T01 MTR_8g064890 35.977 353 154 14 14 351 12 307 9.18e-43 153
MsG0180001502.01.T01 MTR_8g033090 33.237 346 139 9 1 315 1 285 1.03e-42 154
MsG0180001502.01.T01 MTR_8g018110 32.861 353 132 13 1 314 1 287 9.88e-40 145
MsG0180001502.01.T01 MTR_8g036970 33.824 340 114 11 22 351 2 240 4.19e-39 143
MsG0180001502.01.T01 MTR_5g096070 41.121 214 48 5 15 227 23 159 5.17e-39 137
MsG0180001502.01.T01 MTR_6g478230 34.048 373 150 15 9 351 18 324 7.78e-39 144
MsG0180001502.01.T01 MTR_8g046200 32.908 392 168 15 1 351 1 338 1.94e-38 142
MsG0180001502.01.T01 MTR_8g046220 32.821 390 155 13 1 351 1 322 4.67e-38 141
MsG0180001502.01.T01 MTR_1g076810 34.982 283 116 7 1 273 4 228 1.81e-37 143
MsG0180001502.01.T01 MTR_2g104260 36.111 324 120 12 16 310 12 277 2.50e-37 136
MsG0180001502.01.T01 MTR_5g097850 41.791 201 66 4 160 351 5 163 1.37e-36 133
MsG0180001502.01.T01 MTR_5g074800 32.198 323 130 10 57 351 1 262 1.46e-36 135
MsG0180001502.01.T01 MTR_6g070880 33.916 286 109 9 17 276 31 262 2.02e-36 133
MsG0180001502.01.T01 MTR_6g071420 33.916 286 109 9 17 276 31 262 2.02e-36 133
MsG0180001502.01.T01 MTR_5g094350 35.669 314 146 12 15 319 10 276 1.36e-35 131
MsG0180001502.01.T01 MTR_8g073570 38.393 224 82 6 9 230 1 170 4.36e-35 127
MsG0180001502.01.T01 MTR_6g090250 31.593 364 150 12 36 351 29 341 1.07e-33 129
MsG0180001502.01.T01 MTR_1g050308 34.375 256 114 7 17 270 33 236 2.64e-33 127
MsG0180001502.01.T01 MTR_5g092860 32.578 353 120 13 36 351 20 291 3.68e-33 127
MsG0180001502.01.T01 MTR_6g465360 36.145 249 88 8 4 234 45 240 6.61e-33 124
MsG0180001502.01.T01 MTR_5g091150 42.308 182 77 5 163 344 35 188 3.18e-31 117
MsG0180001502.01.T01 MTR_5g097650 39.091 220 61 7 8 220 5 158 5.22e-30 113
MsG0180001502.01.T01 MTR_4g007940 33.121 314 126 14 67 352 3 260 5.76e-30 118
MsG0180001502.01.T01 MTR_5g064700 31.548 336 141 13 57 351 1 288 1.82e-29 117
MsG0180001502.01.T01 MTR_5g049560 40.217 184 69 3 55 234 1 147 2.71e-29 114
MsG0180001502.01.T01 MTR_1g115205 32.986 288 97 11 18 289 29 236 1.22e-27 109
MsG0180001502.01.T01 MTR_8g064790 30.523 344 133 13 15 351 12 256 3.68e-27 110
MsG0180001502.01.T01 MTR_6g078010 31.661 319 129 13 55 351 1 252 3.98e-26 107
MsG0180001502.01.T01 MTR_6g004450 41.844 141 65 5 222 351 52 186 6.29e-25 102
MsG0180001502.01.T01 MTR_5g043605 35.979 189 102 7 173 351 77 256 3.89e-24 102
MsG0180001502.01.T01 MTR_1g050312 30.116 259 112 7 95 351 16 207 1.21e-23 99.8
MsG0180001502.01.T01 MTR_5g097540 52.000 100 45 2 252 351 96 192 3.70e-23 98.6
MsG0180001502.01.T01 MTR_1g047080 29.487 312 141 13 55 358 1 241 7.76e-23 97.4
MsG0180001502.01.T01 MTR_5g068490 35.754 179 97 8 175 351 34 196 2.32e-22 95.9
MsG0180001502.01.T01 MTR_5g022850 34.222 225 102 9 158 351 60 269 7.24e-22 95.9
MsG0180001502.01.T01 MTR_8g041440 51.000 100 26 1 15 114 12 88 7.45e-22 90.1
MsG0180001502.01.T01 MTR_1g098180 50.000 110 44 3 2 109 4 104 8.49e-22 95.9
MsG0180001502.01.T01 MTR_1g098180 33.125 160 84 7 189 341 120 263 5.91e-14 72.8
MsG0180001502.01.T01 MTR_8g033000 28.783 337 143 13 55 351 1 280 9.14e-22 95.9
MsG0180001502.01.T01 MTR_5g076490 29.964 277 120 8 95 351 31 253 4.24e-21 93.6
MsG0180001502.01.T01 MTR_5g096050 56.627 83 23 2 282 351 1 83 5.50e-21 87.8
MsG0180001502.01.T01 MTR_4g014370 43.056 144 63 5 15 154 19 147 2.28e-20 91.3
MsG0180001502.01.T01 MTR_6g087620 35.681 213 91 9 158 351 20 205 5.49e-20 89.4
MsG0180001502.01.T01 MTR_6g079790 35.450 189 93 9 191 351 39 226 5.86e-20 89.7
MsG0180001502.01.T01 MTR_5g023970 43.269 104 54 3 252 351 10 112 4.87e-19 84.7
MsG0180001502.01.T01 MTR_3g019290 46.364 110 50 3 1 109 1 102 9.23e-19 83.6
MsG0180001502.01.T01 MTR_2g021920 37.342 158 74 6 218 351 36 192 1.15e-18 85.5
MsG0180001502.01.T01 MTR_3g018750 33.333 159 74 4 219 351 3 155 3.95e-17 80.5
MsG0180001502.01.T01 MTR_6g009350 37.143 140 70 5 222 347 11 146 5.98e-17 79.0
MsG0180001502.01.T01 MTR_4g016000 38.150 173 77 8 194 352 2 158 1.59e-16 78.2
MsG0180001502.01.T01 MTR_7g023800 27.575 301 139 11 57 351 1 228 6.14e-16 78.2
MsG0180001502.01.T01 MTR_8g063370 29.167 192 114 7 173 351 124 306 1.76e-15 77.8
MsG0180001502.01.T01 MTR_3g047580 51.562 64 31 0 15 78 11 74 3.42e-15 72.0
MsG0180001502.01.T01 MTR_6g090260 36.029 136 76 4 232 361 59 189 3.62e-15 75.1
MsG0180001502.01.T01 MTR_6g090260 58.182 55 22 1 14 67 16 70 8.84e-11 62.4
MsG0180001502.01.T01 MTR_5g098950 37.719 114 62 3 191 295 90 203 3.90e-15 75.1
MsG0180001502.01.T01 MTR_8g035690 29.897 194 95 7 195 351 30 219 5.28e-15 75.5
MsG0180001502.01.T01 MTR_3g058480 32.680 153 94 4 173 322 144 290 1.01e-14 74.7
MsG0180001502.01.T01 MTR_7g017120 41.346 104 56 2 252 351 80 182 2.53e-14 72.8
MsG0180001502.01.T01 MTR_7g017150 40.385 104 57 2 252 351 81 183 8.00e-14 71.2
MsG0180001502.01.T01 MTR_3g047540 44.872 78 41 2 251 326 4 81 9.10e-14 67.0
MsG0180001502.01.T01 MTR_7g019250 35.417 144 64 6 222 352 227 354 2.18e-13 71.6
MsG0180001502.01.T01 MTR_2g028140 22.599 354 196 12 15 346 2 299 3.83e-13 70.9
MsG0180001502.01.T01 MTR_8g063350 26.941 219 131 10 149 351 55 260 4.09e-13 70.1
MsG0180001502.01.T01 MTR_8g063310 30.457 197 115 11 173 351 122 314 5.27e-13 70.1
MsG0180001502.01.T01 MTR_5g021860 25.000 308 203 8 18 317 30 317 7.19e-13 70.1
MsG0180001502.01.T01 MTR_5g021860 25.000 308 203 8 18 317 30 317 7.90e-13 70.1
MsG0180001502.01.T01 MTR_6g087660 44.444 81 43 1 1 79 1 81 9.66e-13 67.0
MsG0180001502.01.T01 MTR_5g096920 48.571 70 34 1 282 351 1 68 1.28e-12 65.5
MsG0180001502.01.T01 MTR_3g007360 32.778 180 108 9 173 341 146 323 1.55e-12 68.9
MsG0180001502.01.T01 MTR_7g101200 32.203 177 86 9 189 351 38 194 1.58e-12 67.0
MsG0180001502.01.T01 MTR_4g027370 24.839 310 205 8 16 317 28 317 1.70e-12 68.9
MsG0180001502.01.T01 MTR_3g007410 32.222 180 109 9 173 341 146 323 4.64e-12 67.4
MsG0180001502.01.T01 MTR_8g041490 41.000 100 27 2 15 114 12 79 5.27e-12 62.4
MsG0180001502.01.T01 MTR_6g027910 26.257 179 111 6 175 335 114 289 9.29e-12 66.2
MsG0180001502.01.T01 MTR_5g086780 60.714 56 19 1 16 68 14 69 1.16e-11 64.3
MsG0180001502.01.T01 MTR_5g022900 25.402 311 202 9 16 317 30 319 7.36e-11 63.9
MsG0180001502.01.T01 MTR_4g133170 27.136 199 109 8 167 340 74 261 7.79e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001502.01.T01 AT3G06240 26.045 311 185 13 18 319 36 310 4.02e-13 70.9

Find 79 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTATCTGTATTCTCTTTC+TGG 0.078612 1:+22356671 MsG0180001502.01.T01:CDS
GTGTGCCATCTGATCAATTT+TGG 0.179842 1:+22357131 MsG0180001502.01.T01:CDS
TGCCCGAGAATAATCCTTCT+TGG 0.252645 1:+22357008 MsG0180001502.01.T01:CDS
ACACGATTAGGGTTAAATTT+TGG 0.252670 1:-22357112 None:intergenic
AATACAGATAATAATAAATT+TGG 0.261393 1:-22356661 None:intergenic
TGATCAGATGGCACACGATT+AGG 0.277260 1:-22357124 None:intergenic
AACAGAAACAAATACGTATT+TGG 0.278603 1:+22357267 MsG0180001502.01.T01:CDS
AAACGCTTCAAAGATTTAAA+AGG 0.279731 1:-22356485 None:intergenic
CTCTTATGTGGATGACAGTT+TGG 0.291726 1:+22357336 MsG0180001502.01.T01:CDS
CGCCTCGTCAAATGTTGTTC+AGG 0.342525 1:+22356403 MsG0180001502.01.T01:CDS
TAATCCTTCTTGGTATGTTT+CGG 0.353019 1:+22357018 MsG0180001502.01.T01:CDS
AACAAGGGTTGTCAAATAAG+AGG 0.367149 1:-22356532 None:intergenic
GATCAGATGGCACACGATTA+GGG 0.376900 1:-22357123 None:intergenic
AGAATAGAGTCAAATTAGAT+TGG 0.387136 1:+22356705 MsG0180001502.01.T01:CDS
GGTATTCTTTGTCTAAAAGC+TGG 0.408632 1:+22356920 MsG0180001502.01.T01:CDS
TCATCATCATCCTCCTCATC+TGG 0.413338 1:-22356836 None:intergenic
CCATCATCATCCTCTTGAAA+TGG 0.419953 1:-22356866 None:intergenic
GTCATCCACATAAGAGGGTA+TGG 0.429296 1:-22357329 None:intergenic
AAAGAATACCATTAAAACTA+AGG 0.435281 1:-22356907 None:intergenic
AGTGGTCTTCTCAAAACTAT+AGG 0.436461 1:-22356782 None:intergenic
AGTTTGAATTCGTTGGTAGT+TGG 0.439341 1:-22356977 None:intergenic
CAAACTGTCATCCACATAAG+AGG 0.442626 1:-22357335 None:intergenic
TTAATAACATCAAGTCTCTC+TGG 0.447534 1:-22357372 None:intergenic
AATATCATCTGGTATATGAT+TGG 0.455802 1:-22356438 None:intergenic
AAATTTGGCGTCTCTCTTGT+GGG 0.462152 1:-22356646 None:intergenic
ACATCCTCGGTCAAAACCTT+TGG 0.464888 1:-22356728 None:intergenic
ATAGCCTACGCAGTAACTCT+TGG 0.465302 1:+22357161 MsG0180001502.01.T01:CDS
AGAGACTTGATGTTATTAAA+CGG 0.466511 1:+22357376 MsG0180001502.01.T01:CDS
CCATTTCAAGAGGATGATGA+TGG 0.471596 1:+22356866 MsG0180001502.01.T01:CDS
TCGGCCGATCATTATCAAAT+CGG 0.473206 1:+22357037 MsG0180001502.01.T01:CDS
TAAATTTGGCGTCTCTCTTG+TGG 0.473870 1:-22356647 None:intergenic
TGTCTAAAAGCTGGATTCTA+CGG 0.481457 1:+22356929 MsG0180001502.01.T01:CDS
TGTATTCTCTTTCTGGTGAG+AGG 0.484321 1:+22356678 MsG0180001502.01.T01:CDS
CGTAATCGAGTGTACTTGAA+TGG 0.494817 1:+22357223 MsG0180001502.01.T01:CDS
GGATGTCTATCGACTTCAAA+AGG 0.503568 1:+22356745 MsG0180001502.01.T01:CDS
AGGAATGAGTTTGAATTCGT+TGG 0.503942 1:-22356984 None:intergenic
ATACCAAGAAGGATTATTCT+CGG 0.504259 1:-22357011 None:intergenic
CATCATCTTCTCTAAGATGT+AGG 0.507693 1:-22356622 None:intergenic
AACACCCATACCCTCTTATG+TGG 0.513515 1:+22357324 MsG0180001502.01.T01:CDS
ATCTCCCAAAATTGATCAGA+TGG 0.520160 1:-22357136 None:intergenic
ACTTGAATGGAGTGTCTCAT+TGG 0.521299 1:+22357236 MsG0180001502.01.T01:CDS
TTGAGAAGACCACTGGTACC+AGG 0.527358 1:+22356791 MsG0180001502.01.T01:CDS
ATAGAGAAGACAATATCATC+TGG 0.531548 1:-22356449 None:intergenic
AAGAATACCATTAAAACTAA+GGG 0.540846 1:-22356906 None:intergenic
TACCAAGAAGGATTATTCTC+GGG 0.545050 1:-22357010 None:intergenic
ACCGCCAGTTTGGCTAATAG+TGG 0.553295 1:-22357510 None:intergenic
TTGAATGTGTACGTAAACCT+TGG 0.554355 1:+22356507 MsG0180001502.01.T01:CDS
GAAGGATTATTCTCGGGCAA+AGG 0.568316 1:-22357004 None:intergenic
TCCTCATCTGGATGAACATG+TGG 0.576591 1:-22356824 None:intergenic
CGGTACATAGTCATGAAACA+AGG 0.578691 1:-22356548 None:intergenic
ACAAGGGTTGTCAAATAAGA+GGG 0.590344 1:-22356531 None:intergenic
TGAATGGAGTGTCTCATTGG+TGG 0.594828 1:+22357239 MsG0180001502.01.T01:CDS
GCGAAACGAGAGTTGTATTG+TGG 0.596166 1:+22356951 MsG0180001502.01.T01:CDS
ACATGTGGAACAAACCATCC+TGG 0.600545 1:-22356809 None:intergenic
TCATCCACATAAGAGGGTAT+GGG 0.612470 1:-22357328 None:intergenic
AAACTGTCATCCACATAAGA+GGG 0.612590 1:-22357334 None:intergenic
AGTCAAATTAGATTGGCCAA+AGG 0.619489 1:+22356712 MsG0180001502.01.T01:CDS
TCTTCCAAGAGTTACTGCGT+AGG 0.620101 1:-22357165 None:intergenic
ACATGTTCATCCAGATGAGG+AGG 0.621711 1:+22356826 MsG0180001502.01.T01:CDS
GAATGGAGTGTCTCATTGGT+GGG 0.622509 1:+22357240 MsG0180001502.01.T01:CDS
ATAACCGATTTGATAATGAT+CGG 0.624831 1:-22357041 None:intergenic
AATATGCAAAATTTAGCCGC+CGG 0.627384 1:-22357471 None:intergenic
TCCACATGTTCATCCAGATG+AGG 0.627843 1:+22356823 MsG0180001502.01.T01:CDS
GTCTTCTCAAAACTATAGGA+CGG 0.629432 1:-22356778 None:intergenic
TTGAAGTCGATAGACATCCT+CGG 0.634485 1:-22356741 None:intergenic
TTGTCAAATAAGAGGGACCA+AGG 0.634881 1:-22356524 None:intergenic
GCCAGTTTGGCTAATAGTGG+CGG 0.637158 1:-22357507 None:intergenic
GGTGTCGTTTGACTTCTGCA+AGG 0.643769 1:+22357288 MsG0180001502.01.T01:CDS
AGAATACCATTAAAACTAAG+GGG 0.648935 1:-22356905 None:intergenic
GAAGACCACTGGTACCAGGA+TGG 0.650110 1:+22356795 MsG0180001502.01.T01:CDS
ACAAACCATCCTGGTACCAG+TGG 0.656773 1:-22356800 None:intergenic
TCGGCCGAAACATACCAAGA+AGG 0.660122 1:-22357022 None:intergenic
TGACGACGGACCATTTCAAG+AGG 0.660532 1:+22356856 MsG0180001502.01.T01:CDS
CTGAACAACATTTGACGAGG+CGG 0.673425 1:-22356402 None:intergenic
GTATCATCATAATAAGAACA+TGG 0.689910 1:-22356590 None:intergenic
GAGGAGGATGATGATGACGA+CGG 0.692169 1:+22356842 MsG0180001502.01.T01:CDS
AACCTGAACAACATTTGACG+AGG 0.701887 1:-22356405 None:intergenic
GGTACATAGTCATGAAACAA+GGG 0.721899 1:-22356547 None:intergenic
GTCTTGTTAAAAGATAGTTG+CGG 0.724702 1:-22356568 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATACAGATAATAATAAATT+TGG - Chr1:22356664-22356683 None:intergenic 10.0%
!! AAAGAATACCATTAAAACTA+AGG - Chr1:22356910-22356929 None:intergenic 20.0%
!! AAGAATACCATTAAAACTAA+GGG - Chr1:22356909-22356928 None:intergenic 20.0%
! AAACGCTTCAAAGATTTAAA+AGG - Chr1:22356488-22356507 None:intergenic 25.0%
! AACAGAAACAAATACGTATT+TGG + Chr1:22357267-22357286 MsG0180001502.01.T01:CDS 25.0%
! AATATCATCTGGTATATGAT+TGG - Chr1:22356441-22356460 None:intergenic 25.0%
! AGAATACCATTAAAACTAAG+GGG - Chr1:22356908-22356927 None:intergenic 25.0%
! AGAATAGAGTCAAATTAGAT+TGG + Chr1:22356705-22356724 MsG0180001502.01.T01:CDS 25.0%
! AGAGACTTGATGTTATTAAA+CGG + Chr1:22357376-22357395 MsG0180001502.01.T01:CDS 25.0%
! GTATCATCATAATAAGAACA+TGG - Chr1:22356593-22356612 None:intergenic 25.0%
! TATTATCTGTATTCTCTTTC+TGG + Chr1:22356671-22356690 MsG0180001502.01.T01:CDS 25.0%
!!! ATAACCGATTTGATAATGAT+CGG - Chr1:22357044-22357063 None:intergenic 25.0%
ATACCAAGAAGGATTATTCT+CGG - Chr1:22357014-22357033 None:intergenic 30.0%
ATAGAGAAGACAATATCATC+TGG - Chr1:22356452-22356471 None:intergenic 30.0%
TAATCCTTCTTGGTATGTTT+CGG + Chr1:22357018-22357037 MsG0180001502.01.T01:CDS 30.0%
TTAATAACATCAAGTCTCTC+TGG - Chr1:22357375-22357394 None:intergenic 30.0%
! ACACGATTAGGGTTAAATTT+TGG - Chr1:22357115-22357134 None:intergenic 30.0%
! GTCTTGTTAAAAGATAGTTG+CGG - Chr1:22356571-22356590 None:intergenic 30.0%
!!! TGACAGTTTGGATTTTAACT+TGG + Chr1:22357348-22357367 MsG0180001502.01.T01:CDS 30.0%
AAACTGTCATCCACATAAGA+GGG - Chr1:22357337-22357356 None:intergenic 35.0%
AACAAGGGTTGTCAAATAAG+AGG - Chr1:22356535-22356554 None:intergenic 35.0%
AATATGCAAAATTTAGCCGC+CGG - Chr1:22357474-22357493 None:intergenic 35.0%
ACAAAAAAACGCGTGAATTC+CGG + Chr1:22357452-22357471 MsG0180001502.01.T01:CDS 35.0%
ACAAGGGTTGTCAAATAAGA+GGG - Chr1:22356534-22356553 None:intergenic 35.0%
AGGAATGAGTTTGAATTCGT+TGG - Chr1:22356987-22357006 None:intergenic 35.0%
AGTGGTCTTCTCAAAACTAT+AGG - Chr1:22356785-22356804 None:intergenic 35.0%
ATCTCCCAAAATTGATCAGA+TGG - Chr1:22357139-22357158 None:intergenic 35.0%
CGATTACGAATCGAAAAAGA+AGG - Chr1:22357211-22357230 None:intergenic 35.0%
GGTACATAGTCATGAAACAA+GGG - Chr1:22356550-22356569 None:intergenic 35.0%
GGTATTCTTTGTCTAAAAGC+TGG + Chr1:22356920-22356939 MsG0180001502.01.T01:CDS 35.0%
GTCTTCTCAAAACTATAGGA+CGG - Chr1:22356781-22356800 None:intergenic 35.0%
TACCAAGAAGGATTATTCTC+GGG - Chr1:22357013-22357032 None:intergenic 35.0%
TGTCTAAAAGCTGGATTCTA+CGG + Chr1:22356929-22356948 MsG0180001502.01.T01:CDS 35.0%
TTGAATGTGTACGTAAACCT+TGG + Chr1:22356507-22356526 MsG0180001502.01.T01:CDS 35.0%
! AGTCAAATTAGATTGGCCAA+AGG + Chr1:22356712-22356731 MsG0180001502.01.T01:CDS 35.0%
! CGGCTAAATTTTGCATATTG+CGG + Chr1:22357475-22357494 MsG0180001502.01.T01:CDS 35.0%
! CTAAATTTTGCATATTGCGG+CGG + Chr1:22357478-22357497 MsG0180001502.01.T01:CDS 35.0%
! TGTGCCATCTGATCAATTTT+GGG + Chr1:22357132-22357151 MsG0180001502.01.T01:CDS 35.0%
!! AGTTTGAATTCGTTGGTAGT+TGG - Chr1:22356980-22356999 None:intergenic 35.0%
!! CATCATCTTCTCTAAGATGT+AGG - Chr1:22356625-22356644 None:intergenic 35.0%
!!! TATAGTTTTGAGAAGACCAC+TGG + Chr1:22356784-22356803 MsG0180001502.01.T01:CDS 35.0%
!!! TCACATCCCCTTAGTTTTAA+TGG + Chr1:22356899-22356918 MsG0180001502.01.T01:CDS 35.0%
AAATTTGGCGTCTCTCTTGT+GGG - Chr1:22356649-22356668 None:intergenic 40.0%
AACCTGAACAACATTTGACG+AGG - Chr1:22356408-22356427 None:intergenic 40.0%
CAAACTGTCATCCACATAAG+AGG - Chr1:22357338-22357357 None:intergenic 40.0%
CCATCATCATCCTCTTGAAA+TGG - Chr1:22356869-22356888 None:intergenic 40.0%
CCATTTCAAGAGGATGATGA+TGG + Chr1:22356866-22356885 MsG0180001502.01.T01:CDS 40.0%
CGGTACATAGTCATGAAACA+AGG - Chr1:22356551-22356570 None:intergenic 40.0%
CGTAATCGAGTGTACTTGAA+TGG + Chr1:22357223-22357242 MsG0180001502.01.T01:CDS 40.0%
CTCTTATGTGGATGACAGTT+TGG + Chr1:22357336-22357355 MsG0180001502.01.T01:CDS 40.0%
TAAATTTGGCGTCTCTCTTG+TGG - Chr1:22356650-22356669 None:intergenic 40.0%
TCGGCCGATCATTATCAAAT+CGG + Chr1:22357037-22357056 MsG0180001502.01.T01:CDS 40.0%
TTGAAGTCGATAGACATCCT+CGG - Chr1:22356744-22356763 None:intergenic 40.0%
TTGTCAAATAAGAGGGACCA+AGG - Chr1:22356527-22356546 None:intergenic 40.0%
! ACTTGAATGGAGTGTCTCAT+TGG + Chr1:22357236-22357255 MsG0180001502.01.T01:CDS 40.0%
! GTGTGCCATCTGATCAATTT+TGG + Chr1:22357131-22357150 MsG0180001502.01.T01:CDS 40.0%
! TCATCCACATAAGAGGGTAT+GGG - Chr1:22357331-22357350 None:intergenic 40.0%
! TGTATTCTCTTTCTGGTGAG+AGG + Chr1:22356678-22356697 MsG0180001502.01.T01:CDS 40.0%
!! GGATGTCTATCGACTTCAAA+AGG + Chr1:22356745-22356764 MsG0180001502.01.T01:CDS 40.0%
AAAAAACGCGTGAATTCCGG+CGG + Chr1:22357455-22357474 MsG0180001502.01.T01:CDS 45.0%
AACACCCATACCCTCTTATG+TGG + Chr1:22357324-22357343 MsG0180001502.01.T01:CDS 45.0%
ACATCCTCGGTCAAAACCTT+TGG - Chr1:22356731-22356750 None:intergenic 45.0%
ACATGTGGAACAAACCATCC+TGG - Chr1:22356812-22356831 None:intergenic 45.0%
ACATGTTCATCCAGATGAGG+AGG + Chr1:22356826-22356845 MsG0180001502.01.T01:CDS 45.0%
ATAGCCTACGCAGTAACTCT+TGG + Chr1:22357161-22357180 MsG0180001502.01.T01:CDS 45.0%
CTGAACAACATTTGACGAGG+CGG - Chr1:22356405-22356424 None:intergenic 45.0%
GAAGGATTATTCTCGGGCAA+AGG - Chr1:22357007-22357026 None:intergenic 45.0%
GATCAGATGGCACACGATTA+GGG - Chr1:22357126-22357145 None:intergenic 45.0%
GCGAAACGAGAGTTGTATTG+TGG + Chr1:22356951-22356970 MsG0180001502.01.T01:CDS 45.0%
GTCATCCACATAAGAGGGTA+TGG - Chr1:22357332-22357351 None:intergenic 45.0%
TCATCATCATCCTCCTCATC+TGG - Chr1:22356839-22356858 None:intergenic 45.0%
TCCACATGTTCATCCAGATG+AGG + Chr1:22356823-22356842 MsG0180001502.01.T01:CDS 45.0%
TCCTCATCTGGATGAACATG+TGG - Chr1:22356827-22356846 None:intergenic 45.0%
TCTTCCAAGAGTTACTGCGT+AGG - Chr1:22357168-22357187 None:intergenic 45.0%
TGATCAGATGGCACACGATT+AGG - Chr1:22357127-22357146 None:intergenic 45.0%
TGCCCGAGAATAATCCTTCT+TGG + Chr1:22357008-22357027 MsG0180001502.01.T01:CDS 45.0%
! GAATGGAGTGTCTCATTGGT+GGG + Chr1:22357240-22357259 MsG0180001502.01.T01:CDS 45.0%
! TGAATGGAGTGTCTCATTGG+TGG + Chr1:22357239-22357258 MsG0180001502.01.T01:CDS 45.0%
ACAAACCATCCTGGTACCAG+TGG - Chr1:22356803-22356822 None:intergenic 50.0%
CGCCTCGTCAAATGTTGTTC+AGG + Chr1:22356403-22356422 MsG0180001502.01.T01:CDS 50.0%
TCGGCCGAAACATACCAAGA+AGG - Chr1:22357025-22357044 None:intergenic 50.0%
TGACGACGGACCATTTCAAG+AGG + Chr1:22356856-22356875 MsG0180001502.01.T01:CDS 50.0%
TTGAGAAGACCACTGGTACC+AGG + Chr1:22356791-22356810 MsG0180001502.01.T01:CDS 50.0%
! GAGGAGGATGATGATGACGA+CGG + Chr1:22356842-22356861 MsG0180001502.01.T01:CDS 50.0%
!! GGTGTCGTTTGACTTCTGCA+AGG + Chr1:22357288-22357307 MsG0180001502.01.T01:CDS 50.0%
!! TTGGCCAAAGGTTTTGACCG+AGG + Chr1:22356724-22356743 MsG0180001502.01.T01:CDS 50.0%
GAAGACCACTGGTACCAGGA+TGG + Chr1:22356795-22356814 MsG0180001502.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 22356395 22357519 22356395 ID=MsG0180001502.01;Name=MsG0180001502.01
Chr1 mRNA 22356395 22357519 22356395 ID=MsG0180001502.01.T01;Parent=MsG0180001502.01;Name=MsG0180001502.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|374
Chr1 exon 22356395 22357519 22356395 ID=MsG0180001502.01.T01:exon:15873;Parent=MsG0180001502.01.T01
Chr1 CDS 22356395 22357519 22356395 ID=MsG0180001502.01.T01:cds;Parent=MsG0180001502.01.T01
Gene Sequence

>MsG0180001502.01.T01

ATGTCATCCGCCTCGTCAAATGTTGTTCAGGTTAGCAGCAATGCCAATCATATACCAGATGATATTGTCTTCTCTATTCTCTCTAAACTTCCTTTTAAATCTTTGAAGCGTTTTGAATGTGTACGTAAACCTTGGTCCCTCTTATTTGACAACCCTTGTTTCATGACTATGTACCGCAACTATCTTTTAACAAGACCATGTTCTTATTATGATGATACATCTCTTCTCCTACATCTTAGAGAAGATGATGACCCACAAGAGAGACGCCAAATTTATTATTATCTGTATTCTCTTTCTGGTGAGAGGTTTGAGAATAGAGTCAAATTAGATTGGCCAAAGGTTTTGACCGAGGATGTCTATCGACTTCAAAAGGAACAGTGTGTCCGTCCTATAGTTTTGAGAAGACCACTGGTACCAGGATGGTTTGTTCCACATGTTCATCCAGATGAGGAGGATGATGATGACGACGGACCATTTCAAGAGGATGATGATGGTTTTGAAATTTCACATCCCCTTAGTTTTAATGGTATTCTTTGTCTAAAAGCTGGATTCTACGGCGAAACGAGAGTTGTATTGTGGAATCCAACTACCAACGAATTCAAACTCATTCCTTTGCCCGAGAATAATCCTTCTTGGTATGTTTCGGCCGATCATTATCAAATCGGTTATGATCATGTTAAAGATGATTATAAGATGATTCGCTGCACATATTATTCTCCAAAATTTAACCCTAATCGTGTGCCATCTGATCAATTTTGGGAGATCTATAGCCTACGCAGTAACTCTTGGAAGAAAATTAATGTCGACATGACTCCTTCTTTTTCGATTCGTAATCGAGTGTACTTGAATGGAGTGTCTCATTGGTGGGATAAAACAGAAACAAATACGTATTTGGTGTCGTTTGACTTCTGCAAGGAATCTTTTATTACAACACCCATACCCTCTTATGTGGATGACAGTTTGGATTTTAACTTGGTCCAGAGAGACTTGATGTTATTAAACGGATCTGTTGCTTTTATGTTACATTATACAGAGACATCAACAATTTACATTCACTACAAAAAAACGCGTGAATTCCGGCGGCTAAATTTTGCATATTGCGGCGGTTTTAGCCGCCACTATTAG
Protein sequence

>MsG0180001502.01.T01

MSSASSNVVQVSSNANHIPDDIVFSILSKLPFKSLKRFECVRKPWSLLFDNPCFMTMYRNYLLTRPCSYYDDTSLLLHLREDDDPQERRQIYYYLYSLSGERFENRVKLDWPKVLTEDVYRLQKEQCVRPIVLRRPLVPGWFVPHVHPDEEDDDDDGPFQEDDDGFEISHPLSFNGILCLKAGFYGETRVVLWNPTTNEFKLIPLPENNPSWYVSADHYQIGYDHVKDDYKMIRCTYYSPKFNPNRVPSDQFWEIYSLRSNSWKKINVDMTPSFSIRNRVYLNGVSHWWDKTETNTYLVSFDFCKESFITTPIPSYVDDSLDFNLVQRDLMLLNGSVAFMLHYTETSTIYIHYKKTREFRRLNFAYCGGFSRHY*