AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031306.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031306.01.T01 MTR_5g069390 57.186 334 128 6 20 348 1 324 1.50e-122 362
MsG0680031306.01.T01 MTR_5g037680 59.425 313 100 7 37 348 3 289 3.62e-118 349
MsG0680031306.01.T01 MTR_5g059430 54.810 343 128 10 20 347 1 331 1.44e-115 342
MsG0680031306.01.T01 MTR_5g097820 58.904 292 93 9 20 307 1 269 3.77e-110 322
MsG0680031306.01.T01 MTR_7g017170 42.679 321 152 10 33 347 9 303 1.97e-74 236
MsG0680031306.01.T01 MTR_7g017130 42.679 321 152 10 33 347 9 303 2.34e-74 235
MsG0680031306.01.T01 MTR_2g022000 44.379 338 153 12 27 347 4 323 1.20e-73 234
MsG0680031306.01.T01 MTR_4g055820 44.207 328 151 13 31 347 3 309 9.33e-73 230
MsG0680031306.01.T01 MTR_6g086825 42.687 335 155 12 26 347 56 366 6.67e-71 228
MsG0680031306.01.T01 MTR_5g097330 44.207 328 146 14 33 348 7 309 2.05e-70 225
MsG0680031306.01.T01 MTR_5g096900 44.675 338 137 15 29 353 2 302 1.32e-69 221
MsG0680031306.01.T01 MTR_1g007410 42.486 346 153 14 14 347 1 312 1.42e-69 223
MsG0680031306.01.T01 MTR_5g097350 44.518 301 133 11 51 347 25 295 1.51e-68 220
MsG0680031306.01.T01 MTR_8g063440 43.385 325 145 12 26 347 1 289 1.22e-67 218
MsG0680031306.01.T01 MTR_2g021800 42.012 338 162 12 27 347 4 324 1.28e-67 218
MsG0680031306.01.T01 MTR_8g012090 41.667 324 113 10 37 347 11 271 1.43e-67 216
MsG0680031306.01.T01 MTR_4g102410 42.229 341 158 11 18 347 42 354 1.07e-66 217
MsG0680031306.01.T01 MTR_2g038680 43.491 338 142 11 32 348 9 318 1.37e-66 216
MsG0680031306.01.T01 MTR_7g069550 43.344 323 148 13 36 347 8 306 2.35e-66 214
MsG0680031306.01.T01 MTR_4g117550 42.173 313 148 10 20 327 1 285 4.50e-66 213
MsG0680031306.01.T01 MTR_7g017040 41.945 329 146 11 33 347 7 304 5.43e-66 214
MsG0680031306.01.T01 MTR_5g096630 42.042 333 166 11 20 347 1 311 1.04e-65 213
MsG0680031306.01.T01 MTR_0129s0070 41.916 334 149 14 26 347 2 302 4.70e-65 211
MsG0680031306.01.T01 MTR_6g090180 40.230 348 172 12 14 347 19 344 9.56e-65 212
MsG0680031306.01.T01 MTR_8g464770 43.631 314 142 12 36 347 6 286 2.62e-64 209
MsG0680031306.01.T01 MTR_2g021770 43.125 320 154 11 40 347 10 313 4.07e-64 209
MsG0680031306.01.T01 MTR_7g016930 40.823 316 137 10 36 347 10 279 1.01e-63 206
MsG0680031306.01.T01 MTR_8g013000 41.896 327 149 13 34 347 2 300 1.25e-63 208
MsG0680031306.01.T01 MTR_5g096910 40.483 331 140 10 35 349 10 299 9.12e-63 205
MsG0680031306.01.T01 MTR_0129s0120 41.617 334 150 14 26 347 2 302 1.02e-62 205
MsG0680031306.01.T01 MTR_2g064950 42.073 328 148 14 35 350 3 300 3.55e-61 201
MsG0680031306.01.T01 MTR_2g021950 40.367 327 162 14 31 347 2 305 8.93e-61 200
MsG0680031306.01.T01 MTR_8g041270 43.413 334 152 13 27 347 3 312 1.33e-60 200
MsG0680031306.01.T01 MTR_6g080680 40.346 347 142 12 20 347 1 301 3.03e-60 199
MsG0680031306.01.T01 MTR_0129s0050 39.706 340 157 12 30 347 2 315 2.08e-59 197
MsG0680031306.01.T01 MTR_8g039500 37.224 317 156 9 36 349 6 282 2.44e-59 195
MsG0680031306.01.T01 MTR_7g079640 41.212 330 144 11 26 350 1 285 5.88e-59 195
MsG0680031306.01.T01 MTR_0054s0090 39.645 338 157 13 27 347 5 312 7.49e-59 196
MsG0680031306.01.T01 MTR_2g104340 41.071 336 150 16 26 347 1 302 7.93e-59 195
MsG0680031306.01.T01 MTR_0134s0060 40.762 341 150 15 32 347 5 318 1.77e-58 194
MsG0680031306.01.T01 MTR_2g104280 39.628 323 163 12 37 347 9 311 3.27e-58 194
MsG0680031306.01.T01 MTR_6g461660 40.187 321 157 10 36 347 13 307 6.63e-58 193
MsG0680031306.01.T01 MTR_0054s0100 39.067 343 152 14 27 346 5 313 8.23e-58 193
MsG0680031306.01.T01 MTR_4g028750 38.484 343 145 15 31 347 2 304 1.28e-57 192
MsG0680031306.01.T01 MTR_6g079810 40.491 326 150 14 41 347 69 369 1.60e-57 193
MsG0680031306.01.T01 MTR_5g097650 55.615 187 51 7 20 202 1 159 4.15e-57 183
MsG0680031306.01.T01 MTR_3g011120 39.809 314 142 14 41 347 13 286 5.12e-57 189
MsG0680031306.01.T01 MTR_5g024260 37.462 331 165 11 26 347 5 302 1.97e-56 189
MsG0680031306.01.T01 MTR_4g028680 39.181 342 146 14 31 347 2 306 2.25e-56 189
MsG0680031306.01.T01 MTR_1g007450 40.184 326 157 13 32 347 5 302 5.06e-56 189
MsG0680031306.01.T01 MTR_4g028510 38.123 341 153 16 31 347 2 308 1.37e-55 188
MsG0680031306.01.T01 MTR_5g072060 39.815 324 130 13 40 347 4 278 3.31e-55 191
MsG0680031306.01.T01 MTR_6g478230 37.322 351 157 13 20 346 1 312 5.54e-55 186
MsG0680031306.01.T01 MTR_4g028660 36.982 338 158 13 31 347 2 305 1.80e-54 184
MsG0680031306.01.T01 MTR_4g027450 36.982 338 158 13 31 347 2 305 1.80e-54 184
MsG0680031306.01.T01 MTR_5g097680 38.551 345 166 14 33 347 8 336 2.22e-54 184
MsG0680031306.01.T01 MTR_6g079770 37.719 342 160 15 33 347 8 323 2.51e-54 184
MsG0680031306.01.T01 MTR_5g097220 37.647 340 160 11 31 347 2 312 9.65e-54 182
MsG0680031306.01.T01 MTR_4g130330 37.195 328 156 12 37 347 2 296 1.02e-53 182
MsG0680031306.01.T01 MTR_4g014370 36.257 342 163 10 33 347 2 315 3.66e-52 178
MsG0680031306.01.T01 MTR_2g066170 35.127 316 169 10 36 347 6 289 9.35e-52 176
MsG0680031306.01.T01 MTR_2g066200 34.675 323 169 11 33 347 3 291 1.81e-51 176
MsG0680031306.01.T01 MTR_7g104710 38.462 338 137 14 20 347 1 277 6.57e-51 172
MsG0680031306.01.T01 MTR_5g037190 39.298 285 124 10 33 309 7 250 8.22e-51 170
MsG0680031306.01.T01 MTR_0831s0010 36.196 326 169 10 28 348 37 328 4.72e-50 173
MsG0680031306.01.T01 MTR_2g065860 35.562 329 168 12 26 347 1 292 7.60e-50 171
MsG0680031306.01.T01 MTR_8g467670 34.985 323 166 12 30 347 2 285 1.18e-49 171
MsG0680031306.01.T01 MTR_2g034700 37.621 311 149 11 40 347 12 280 1.48e-49 170
MsG0680031306.01.T01 MTR_1g053510 37.971 345 160 14 26 348 1 313 3.01e-49 170
MsG0680031306.01.T01 MTR_8g063330 35.093 322 161 11 41 348 13 300 7.11e-49 171
MsG0680031306.01.T01 MTR_1g007430 39.000 300 144 12 53 347 7 272 2.75e-48 166
MsG0680031306.01.T01 MTR_8g064890 36.499 337 156 14 26 347 1 294 4.35e-48 167
MsG0680031306.01.T01 MTR_7g111700 37.188 320 129 13 41 348 14 273 5.37e-48 166
MsG0680031306.01.T01 MTR_8g046310 37.952 332 146 14 28 348 2 284 1.74e-47 165
MsG0680031306.01.T01 MTR_5g094350 37.374 297 143 8 40 324 11 276 2.78e-46 159
MsG0680031306.01.T01 MTR_6g070880 39.370 254 114 10 31 265 24 256 1.48e-45 157
MsG0680031306.01.T01 MTR_6g071420 39.370 254 114 10 31 265 24 256 1.48e-45 157
MsG0680031306.01.T01 MTR_2g066150 33.333 312 153 10 36 347 6 262 1.90e-45 159
MsG0680031306.01.T01 MTR_5g074850 37.356 348 150 19 26 347 23 328 2.03e-45 166
MsG0680031306.01.T01 MTR_2g065840 32.931 331 173 14 26 347 1 291 2.30e-45 160
MsG0680031306.01.T01 MTR_8g033140 34.615 364 151 14 27 347 4 323 3.01e-45 160
MsG0680031306.01.T01 MTR_5g091140 38.209 335 150 15 26 347 31 321 1.51e-44 158
MsG0680031306.01.T01 MTR_1g076810 41.350 237 105 9 37 264 13 224 1.56e-44 162
MsG0680031306.01.T01 MTR_5g075370 37.725 334 147 18 40 347 6 304 2.07e-43 160
MsG0680031306.01.T01 MTR_2g104260 38.908 293 143 13 36 315 8 277 2.70e-43 151
MsG0680031306.01.T01 MTR_8g033090 34.024 338 163 13 40 347 12 319 3.77e-43 154
MsG0680031306.01.T01 MTR_6g090250 35.890 326 147 14 60 347 29 330 7.62e-43 154
MsG0680031306.01.T01 MTR_8g046200 36.467 351 157 12 34 348 8 328 1.07e-42 153
MsG0680031306.01.T01 MTR_5g092850 37.048 332 143 13 41 347 15 305 4.81e-42 151
MsG0680031306.01.T01 MTR_1g098180 31.269 323 158 9 27 348 4 263 6.09e-42 150
MsG0680031306.01.T01 MTR_8g033110 35.000 340 159 14 40 347 12 321 1.21e-41 150
MsG0680031306.01.T01 MTR_5g074800 38.434 281 129 12 81 347 1 251 6.53e-41 147
MsG0680031306.01.T01 MTR_8g032980 32.075 371 159 15 26 347 1 327 7.45e-41 148
MsG0680031306.01.T01 MTR_1g050308 37.143 245 118 8 26 265 23 236 2.41e-40 145
MsG0680031306.01.T01 MTR_5g092860 36.977 311 123 14 60 347 20 280 6.06e-40 145
MsG0680031306.01.T01 MTR_8g018110 35.390 308 143 11 40 321 12 289 2.78e-39 144
MsG0680031306.01.T01 MTR_8g036970 33.003 303 127 10 46 347 2 229 1.14e-37 139
MsG0680031306.01.T01 MTR_8g073570 39.779 181 93 4 38 218 6 170 1.16e-37 133
MsG0680031306.01.T01 MTR_8g046220 32.022 356 165 11 26 348 1 312 6.58e-37 137
MsG0680031306.01.T01 MTR_6g465360 42.512 207 86 10 32 224 55 242 2.52e-36 133
MsG0680031306.01.T01 MTR_6g078010 35.336 283 127 14 79 347 1 241 7.44e-36 133
MsG0680031306.01.T01 MTR_5g064700 35.154 293 148 14 81 347 1 277 7.20e-35 131
MsG0680031306.01.T01 MTR_8g064790 31.385 325 138 12 26 347 1 243 3.89e-34 129
MsG0680031306.01.T01 MTR_1g115205 35.125 279 113 9 27 292 14 237 1.14e-33 125
MsG0680031306.01.T01 MTR_8g063310 30.655 336 183 15 28 351 8 305 3.98e-32 124
MsG0680031306.01.T01 MTR_8g063370 31.429 315 170 15 39 347 21 295 6.03e-32 124
MsG0680031306.01.T01 MTR_5g094360 52.000 125 50 4 185 307 40 156 6.54e-32 117
MsG0680031306.01.T01 MTR_5g022850 32.822 326 136 14 32 347 6 258 8.40e-32 122
MsG0680031306.01.T01 MTR_6g079790 36.444 225 117 8 137 347 3 215 1.01e-30 119
MsG0680031306.01.T01 MTR_5g076490 34.737 285 127 11 79 347 1 242 7.92e-30 117
MsG0680031306.01.T01 MTR_5g096070 38.743 191 74 7 25 215 12 159 5.37e-29 110
MsG0680031306.01.T01 MTR_5g086590 37.791 172 87 6 176 347 14 165 2.07e-28 110
MsG0680031306.01.T01 MTR_4g007940 31.136 273 143 9 92 347 4 248 2.19e-28 113
MsG0680031306.01.T01 MTR_6g087620 35.714 238 98 9 119 346 1 193 3.25e-28 111
MsG0680031306.01.T01 MTR_4g014370 31.329 316 131 10 33 347 13 243 6.55e-28 112
MsG0680031306.01.T01 MTR_3g019290 33.333 222 86 5 40 254 12 178 1.37e-26 104
MsG0680031306.01.T01 MTR_1g050312 34.894 235 95 14 118 347 15 196 7.63e-26 105
MsG0680031306.01.T01 MTR_8g041440 52.525 99 24 1 41 139 14 89 2.98e-25 99.0
MsG0680031306.01.T01 MTR_7g023800 34.783 276 112 15 81 347 1 217 2.62e-24 101
MsG0680031306.01.T01 MTR_5g091150 32.479 234 92 9 131 348 2 185 3.53e-24 98.6
MsG0680031306.01.T01 MTR_1g047080 32.714 269 127 13 79 342 1 220 4.98e-24 100
MsG0680031306.01.T01 MTR_5g049560 37.000 200 115 5 79 274 1 193 5.03e-24 99.8
MsG0680031306.01.T01 MTR_6g004450 40.523 153 72 5 199 348 41 177 1.67e-23 98.6
MsG0680031306.01.T01 MTR_5g043605 30.579 242 125 13 115 348 40 246 1.38e-22 97.8
MsG0680031306.01.T01 MTR_8g063350 27.103 321 146 13 36 347 8 249 6.80e-21 92.4
MsG0680031306.01.T01 MTR_5g068490 27.727 220 117 8 128 347 8 185 1.31e-20 90.5
MsG0680031306.01.T01 MTR_4g016000 33.333 174 81 5 180 347 2 146 2.74e-20 88.6
MsG0680031306.01.T01 MTR_2g021920 42.105 152 72 7 206 347 36 181 1.91e-19 87.0
MsG0680031306.01.T01 MTR_3g047580 32.121 165 73 4 26 189 1 127 4.94e-19 82.4
MsG0680031306.01.T01 MTR_6g090260 46.535 101 46 3 248 347 75 168 6.60e-19 85.5
MsG0680031306.01.T01 MTR_6g090260 48.485 66 30 1 26 91 8 69 1.77e-11 64.3
MsG0680031306.01.T01 MTR_2g028160 25.484 310 174 12 40 330 3 274 2.58e-18 85.9
MsG0680031306.01.T01 MTR_6g009350 40.940 149 65 9 207 348 8 140 5.58e-18 81.6
MsG0680031306.01.T01 MTR_2g028130 25.868 317 194 15 39 346 2 286 1.33e-17 84.0
MsG0680031306.01.T01 MTR_5g023970 44.118 102 48 3 247 348 10 102 3.48e-17 79.3
MsG0680031306.01.T01 MTR_3g018750 34.437 151 82 4 207 348 3 145 1.65e-16 78.6
MsG0680031306.01.T01 MTR_4g023080 27.055 292 150 11 41 326 8 242 2.66e-16 79.3
MsG0680031306.01.T01 MTR_2g024110 25.240 313 176 15 42 346 4 266 4.47e-16 79.0
MsG0680031306.01.T01 MTR_4g021785 28.920 287 152 13 41 326 8 243 8.29e-16 78.2
MsG0680031306.01.T01 MTR_7g019250 33.735 166 74 4 189 347 206 342 1.07e-15 78.6
MsG0680031306.01.T01 MTR_5g098950 40.310 129 66 5 177 303 90 209 1.64e-15 76.3
MsG0680031306.01.T01 MTR_7g017150 46.535 101 43 5 248 347 82 172 1.96e-15 75.9
MsG0680031306.01.T01 MTR_1g037540 25.460 326 191 11 45 347 8 304 1.96e-15 77.4
MsG0680031306.01.T01 MTR_3g034130 29.066 289 155 16 41 319 8 256 2.48e-15 76.6
MsG0680031306.01.T01 MTR_0667s0010 23.368 291 174 10 42 324 6 255 4.63e-15 76.3
MsG0680031306.01.T01 MTR_5g097850 37.324 142 68 6 211 347 27 152 4.73e-15 74.3
MsG0680031306.01.T01 MTR_2g426020 23.390 295 172 12 42 322 6 260 4.77e-15 76.3
MsG0680031306.01.T01 MTR_2g426070 25.246 305 174 11 43 329 7 275 5.96e-15 75.9
MsG0680031306.01.T01 MTR_4g023120 26.761 284 165 11 42 325 3 243 6.59e-15 75.5
MsG0680031306.01.T01 MTR_4g133170 24.272 309 177 13 40 340 2 261 9.33e-15 75.1
MsG0680031306.01.T01 MTR_4g023070 26.460 291 171 12 42 332 3 250 1.20e-14 74.7
MsG0680031306.01.T01 MTR_7g017120 45.545 101 44 5 248 347 81 171 1.21e-14 73.6
MsG0680031306.01.T01 MTR_0115s0020 29.758 289 164 12 41 319 9 268 1.66e-14 74.3
MsG0680031306.01.T01 MTR_4g094365 27.875 287 159 12 35 319 1 241 2.11e-14 73.9
MsG0680031306.01.T01 MTR_5g057710 24.468 282 165 9 42 319 46 283 2.59e-14 73.9
MsG0680031306.01.T01 MTR_3g013740 24.422 303 174 12 33 320 35 297 3.31e-14 73.6
MsG0680031306.01.T01 MTR_5g057790 25.085 295 169 10 42 331 46 293 3.83e-14 73.2
MsG0680031306.01.T01 MTR_4g070550 25.000 312 173 14 39 330 2 272 5.05e-14 73.2
MsG0680031306.01.T01 MTR_8g073550 28.889 225 119 7 42 263 30 216 1.52e-13 69.7
MsG0680031306.01.T01 MTR_5g066420 57.971 69 25 2 284 349 1 68 1.52e-13 67.8
MsG0680031306.01.T01 MTR_4g116330 25.850 294 170 12 34 325 15 262 2.99e-13 70.5
MsG0680031306.01.T01 MTR_3g009310 25.676 296 159 10 36 320 36 281 4.72e-13 70.1
MsG0680031306.01.T01 MTR_7g101200 32.022 178 91 9 175 350 38 187 5.23e-13 68.2
MsG0680031306.01.T01 MTR_2g028110 24.104 307 182 13 42 339 4 268 5.47e-13 69.7
MsG0680031306.01.T01 MTR_3g013770 21.918 292 183 8 37 320 24 278 9.78e-13 68.9
MsG0680031306.01.T01 MTR_3g034160 32.018 228 120 10 36 262 3 196 1.28e-12 68.6
MsG0680031306.01.T01 MTR_4g023130 30.921 152 79 5 175 326 76 201 1.63e-12 67.8
MsG0680031306.01.T01 MTR_3g083010 23.003 313 199 13 42 339 6 291 1.80e-12 68.6
MsG0680031306.01.T01 MTR_1g097560 26.349 315 160 14 41 348 35 284 1.84e-12 68.2
MsG0680031306.01.T01 MTR_1g055135 29.965 287 156 15 41 324 12 256 2.57e-12 67.8
MsG0680031306.01.T01 MTR_2g055970 24.534 322 191 14 25 341 193 467 2.62e-12 68.2
MsG0680031306.01.T01 MTR_3g047540 42.353 85 39 4 246 329 4 79 3.37e-12 62.4
MsG0680031306.01.T01 MTR_2g021050 23.344 317 174 12 39 347 2 257 3.52e-12 67.4
MsG0680031306.01.T01 MTR_1g097490 26.621 293 154 13 33 319 42 279 6.61e-12 66.6
MsG0680031306.01.T01 MTR_4g030830 25.329 304 172 13 37 323 4 269 7.75e-12 66.2
MsG0680031306.01.T01 MTR_4g023150 27.517 298 164 12 36 332 4 250 1.00e-11 65.9
MsG0680031306.01.T01 MTR_3g083640 23.667 300 179 14 42 339 55 306 1.27e-11 65.9
MsG0680031306.01.T01 MTR_6g087660 39.091 110 57 4 27 134 5 106 2.02e-11 62.8
MsG0680031306.01.T01 MTR_5g086780 53.623 69 23 3 20 86 1 62 2.38e-11 63.2
MsG0680031306.01.T01 MTR_5g097540 39.216 102 45 4 248 348 97 182 7.08e-11 62.8
MsG0680031306.01.T01 MTR_3g058480 25.000 296 162 12 38 320 35 283 7.43e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031306.01.T01 AT3G16210 26.978 278 143 9 42 319 5 222 1.80e-15 77.0
MsG0680031306.01.T01 AT3G06240 24.484 339 203 14 30 347 24 330 2.68e-15 77.0

Find 68 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAGTTGTATGCTGTTTC+TGG 0.205123 6:-17800545 MsG0680031306.01.T01:CDS
TTCCAAACCAAACTCGTTCT+TGG 0.228814 6:-17800866 MsG0680031306.01.T01:CDS
TCCAAGTGAATATTGTATTA+TGG 0.250614 6:-17800379 MsG0680031306.01.T01:CDS
AATCGCTTCAAAGATTTAAT+AGG 0.258645 6:+17800731 None:intergenic
TATCTTTCAACTCCTCGATA+TGG 0.271855 6:-17800172 MsG0680031306.01.T01:CDS
AGTCACTTGGTGTTATTAAA+TGG 0.277057 6:-17799891 MsG0680031306.01.T01:CDS
ATTAAATCTTTGAAGCGATT+TGG 0.278221 6:-17800728 MsG0680031306.01.T01:CDS
ACCTTAAAATCTTCGGTAGA+TGG 0.278549 6:+17800353 None:intergenic
TCCTTGGAACGGTGGTTTAT+TGG 0.288244 6:-17800576 MsG0680031306.01.T01:CDS
TGGTGAGAGGTTTGAGAATA+GGG 0.295501 6:-17800525 MsG0680031306.01.T01:CDS
TCTATTTCTTTGATCTTTGT+TGG 0.297616 6:-17799867 MsG0680031306.01.T01:CDS
TCTGTTTGTCATCAAGGATT+TGG 0.313542 6:-17800287 MsG0680031306.01.T01:CDS
CGATATGCATCATGAGCTTA+TGG 0.316866 6:-17800105 MsG0680031306.01.T01:CDS
CGATACATAGTCATAAAATA+AGG 0.320038 6:+17800668 None:intergenic
CTGCGTCTAGAGTATAATTC+TGG 0.323837 6:-17800461 MsG0680031306.01.T01:CDS
ACTCGTTCTTGGAGCCAATA+TGG 0.337091 6:-17800855 MsG0680031306.01.T01:CDS
CTCCAAGAACGAGTTTGGTT+TGG 0.337730 6:+17800864 None:intergenic
TATGGAGAGATGCGTGGATT+TGG 0.349856 6:-17800837 MsG0680031306.01.T01:CDS
ATTGGCTCCAAGAACGAGTT+TGG 0.351424 6:+17800859 None:intergenic
CTGGTGAGAGGTTTGAGAAT+AGG 0.353371 6:-17800526 MsG0680031306.01.T01:CDS
TTTCAATCTTGATTCTCGTT+TGG 0.367632 6:-17799919 MsG0680031306.01.T01:CDS
ATCATTCATGTCTGAGGGTA+AGG 0.383213 6:+17799944 None:intergenic
AGAATAGGGTCAAATTTGAT+TGG 0.396484 6:-17800511 MsG0680031306.01.T01:CDS
AATATTTGTTGTCATTTGAC+TGG 0.400364 6:-17799992 MsG0680031306.01.T01:CDS
CAAACAGAAGCTTCCCAATA+AGG 0.407646 6:+17800302 None:intergenic
TGGAATAACCTTAAAATCTT+CGG 0.422113 6:+17800346 None:intergenic
TTATACTCTAGACGCAGATA+TGG 0.438489 6:+17800467 None:intergenic
GGTCGTTCTAACTCAACCTT+TGG 0.442208 6:+17800488 None:intergenic
ATAGTCTAAGAAGTAACTCT+TGG 0.454400 6:-17800142 MsG0680031306.01.T01:CDS
ATCTTTCAACTCCTCGATAT+GGG 0.454948 6:-17800171 MsG0680031306.01.T01:CDS
ATCCACGCATCTCTCCATAT+TGG 0.456681 6:+17800841 None:intergenic
ATGAGAATGCAAAGGTAAGA+AGG 0.467228 6:-17800805 MsG0680031306.01.T01:CDS
GAAGCTTCTGTTTGTCATCA+AGG 0.483762 6:-17800293 MsG0680031306.01.T01:CDS
ACATGGATGGACTCTCTCAC+TGG 0.488586 6:-17800046 MsG0680031306.01.T01:CDS
TCCAATAAACCACCGTTCCA+AGG 0.498762 6:+17800575 None:intergenic
GATACATAGTCATAAAATAA+GGG 0.506822 6:+17800669 None:intergenic
CTGAGGGTAAGGGTGTTGTA+AGG 0.507776 6:+17799955 None:intergenic
AATACAATATTCACTTGGAA+TGG 0.508389 6:+17800383 None:intergenic
ATCTTGATTCTCGTTTGGTG+TGG 0.517189 6:-17799914 MsG0680031306.01.T01:CDS
TCACTTGGAATGGACAGATC+AGG 0.519605 6:+17800393 None:intergenic
ATAGAGAAGAAAAGATCTTG+AGG 0.526764 6:+17800767 None:intergenic
GTTTGGAAAGTGTAATCCGT+TGG 0.532435 6:+17800881 None:intergenic
TGTGACCTTGATGTGCTACC+AGG 0.539033 6:-17800203 MsG0680031306.01.T01:CDS
TTGAAAGATATATTTGTCCC+TGG 0.541932 6:+17800185 None:intergenic
CAATAAGGTCCAGAATCAAA+AGG 0.547930 6:+17800317 None:intergenic
TCGTTTGGTGTGGAGTCACT+TGG 0.549609 6:-17799904 MsG0680031306.01.T01:CDS
GGTCCAGAATCAAAAGGACT+AGG 0.550391 6:+17800323 None:intergenic
TGTATGCTGTTTCTGGTGAG+AGG 0.557239 6:-17800538 MsG0680031306.01.T01:CDS
GGATATGGTAGTGTTGCTCA+TGG 0.563123 6:-17800425 MsG0680031306.01.T01:CDS
GGATGATGAACATTTGTACA+TGG 0.570257 6:-17800084 MsG0680031306.01.T01:CDS
CAGAATGATATTTACTCCAA+CGG 0.570713 6:-17800897 None:intergenic
TCCATAATACAATATTCACT+TGG 0.578742 6:+17800378 None:intergenic
TTGTCCCTGGTAGCACATCA+AGG 0.581507 6:+17800198 None:intergenic
GGTCAAATTTGATTGGCCAA+AGG 0.585401 6:-17800504 MsG0680031306.01.T01:CDS
GAAAGTATCATTCATGTCTG+AGG 0.586462 6:+17799938 None:intergenic
TCATTCATGTCTGAGGGTAA+GGG 0.593297 6:+17799945 None:intergenic
GGATGATGAACAATTGTACA+TGG 0.596335 6:-17800063 MsG0680031306.01.T01:CDS
AAAGTATCATTCATGTCTGA+GGG 0.601818 6:+17799939 None:intergenic
GATGAACAATTGTACATGGA+TGG 0.605417 6:-17800059 MsG0680031306.01.T01:CDS
TTATCAAATAAGAGCGACCA+TGG 0.618617 6:+17800692 None:intergenic
TCGCAACAACTTCTTAACAA+AGG 0.645192 6:-17800648 MsG0680031306.01.T01:CDS
CCAGAATCAAAAGGACTAGG+TGG 0.651550 6:+17800326 None:intergenic
ACTATATATCTCCCATATCG+AGG 0.658487 6:+17800160 None:intergenic
ATCGACAGATGAGAATGCAA+AGG 0.660893 6:-17800813 MsG0680031306.01.T01:CDS
TTGGATGTGTAAGTAAACCA+TGG 0.679080 6:-17800709 MsG0680031306.01.T01:CDS
GTCATTTGACTGGAGCGATG+AGG 0.682186 6:-17799982 MsG0680031306.01.T01:CDS
AGCCAATATGGAGAGATGCG+TGG 0.686311 6:-17800843 MsG0680031306.01.T01:CDS
GTGACCTTGATGTGCTACCA+GGG 0.689786 6:-17800202 MsG0680031306.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATACATAGTCATAAAATAA+GGG + Chr6:17800081-17800100 None:intergenic 20.0%
!!! AATTCTGGTTTTGATATTTT+AGG - Chr6:17800301-17800320 MsG0680031306.01.T01:CDS 20.0%
! AATACAATATTCACTTGGAA+TGG + Chr6:17800367-17800386 None:intergenic 25.0%
! AATCGCTTCAAAGATTTAAT+AGG + Chr6:17800019-17800038 None:intergenic 25.0%
! CGATACATAGTCATAAAATA+AGG + Chr6:17800082-17800101 None:intergenic 25.0%
! TCCAAGTGAATATTGTATTA+TGG - Chr6:17800368-17800387 MsG0680031306.01.T01:CDS 25.0%
! TCCATAATACAATATTCACT+TGG + Chr6:17800372-17800391 None:intergenic 25.0%
! TGGAATAACCTTAAAATCTT+CGG + Chr6:17800404-17800423 None:intergenic 25.0%
!! TCTATTTCTTTGATCTTTGT+TGG - Chr6:17800880-17800899 MsG0680031306.01.T01:CDS 25.0%
!!! AATATTTGTTGTCATTTGAC+TGG - Chr6:17800755-17800774 MsG0680031306.01.T01:CDS 25.0%
!!! ATTAAATCTTTGAAGCGATT+TGG - Chr6:17800019-17800038 MsG0680031306.01.T01:CDS 25.0%
!!! GGTTTTGATATTTTAGGATA+TGG - Chr6:17800307-17800326 MsG0680031306.01.T01:CDS 25.0%
AGAATAGGGTCAAATTTGAT+TGG - Chr6:17800236-17800255 MsG0680031306.01.T01:CDS 30.0%
ATAGAGAAGAAAAGATCTTG+AGG + Chr6:17799983-17800002 None:intergenic 30.0%
ATAGTCTAAGAAGTAACTCT+TGG - Chr6:17800605-17800624 MsG0680031306.01.T01:CDS 30.0%
TTGAAAGATATATTTGTCCC+TGG + Chr6:17800565-17800584 None:intergenic 30.0%
TTTCAATCTTGATTCTCGTT+TGG - Chr6:17800828-17800847 MsG0680031306.01.T01:CDS 30.0%
! AAAGTATCATTCATGTCTGA+GGG + Chr6:17800811-17800830 None:intergenic 30.0%
! TACATCAAACATTTTGTCCT+TGG - Chr6:17800155-17800174 MsG0680031306.01.T01:CDS 30.0%
!! AGTCACTTGGTGTTATTAAA+TGG - Chr6:17800856-17800875 MsG0680031306.01.T01:CDS 30.0%
!! TTTTGATTCTGGACCTTATT+GGG - Chr6:17800432-17800451 MsG0680031306.01.T01:CDS 30.0%
ACCTTAAAATCTTCGGTAGA+TGG + Chr6:17800397-17800416 None:intergenic 35.0%
ACTATATATCTCCCATATCG+AGG + Chr6:17800590-17800609 None:intergenic 35.0%
ATCTTTCAACTCCTCGATAT+GGG - Chr6:17800576-17800595 MsG0680031306.01.T01:CDS 35.0%
ATGAGAATGCAAAGGTAAGA+AGG - Chr6:17799942-17799961 MsG0680031306.01.T01:CDS 35.0%
CAATAAGGTCCAGAATCAAA+AGG + Chr6:17800433-17800452 None:intergenic 35.0%
GATGAACAATTGTACATGGA+TGG - Chr6:17800688-17800707 MsG0680031306.01.T01:CDS 35.0%
GGATGATGAACAATTGTACA+TGG - Chr6:17800684-17800703 MsG0680031306.01.T01:CDS 35.0%
GGATGATGAACATTTGTACA+TGG - Chr6:17800663-17800682 MsG0680031306.01.T01:CDS 35.0%
TATCTTTCAACTCCTCGATA+TGG - Chr6:17800575-17800594 MsG0680031306.01.T01:CDS 35.0%
TCGCAACAACTTCTTAACAA+AGG - Chr6:17800099-17800118 MsG0680031306.01.T01:CDS 35.0%
TCTGTTTGTCATCAAGGATT+TGG - Chr6:17800460-17800479 MsG0680031306.01.T01:CDS 35.0%
TTATACTCTAGACGCAGATA+TGG + Chr6:17800283-17800302 None:intergenic 35.0%
TTATCAAATAAGAGCGACCA+TGG + Chr6:17800058-17800077 None:intergenic 35.0%
TTGGATGTGTAAGTAAACCA+TGG - Chr6:17800038-17800057 MsG0680031306.01.T01:CDS 35.0%
! CAAACATTTTGTCCTTGGAA+CGG - Chr6:17800160-17800179 MsG0680031306.01.T01:CDS 35.0%
! TCCATCTACCGAAGATTTTA+AGG - Chr6:17800393-17800412 MsG0680031306.01.T01:CDS 35.0%
!! CTTTTGATTCTGGACCTTAT+TGG - Chr6:17800431-17800450 MsG0680031306.01.T01:CDS 35.0%
!! GAAAGTATCATTCATGTCTG+AGG + Chr6:17800812-17800831 None:intergenic 35.0%
!! TTTGAGTTGTATGCTGTTTC+TGG - Chr6:17800202-17800221 MsG0680031306.01.T01:CDS 35.0%
ATCATTCATGTCTGAGGGTA+AGG + Chr6:17800806-17800825 None:intergenic 40.0%
ATCGACAGATGAGAATGCAA+AGG - Chr6:17799934-17799953 MsG0680031306.01.T01:CDS 40.0%
CAAACAGAAGCTTCCCAATA+AGG + Chr6:17800448-17800467 None:intergenic 40.0%
CGATATGCATCATGAGCTTA+TGG - Chr6:17800642-17800661 MsG0680031306.01.T01:CDS 40.0%
GAAGCTTCTGTTTGTCATCA+AGG - Chr6:17800454-17800473 MsG0680031306.01.T01:CDS 40.0%
TCATTCATGTCTGAGGGTAA+GGG + Chr6:17800805-17800824 None:intergenic 40.0%
TGGTGAGAGGTTTGAGAATA+GGG - Chr6:17800222-17800241 MsG0680031306.01.T01:CDS 40.0%
TTCCAAACCAAACTCGTTCT+TGG - Chr6:17799881-17799900 MsG0680031306.01.T01:CDS 40.0%
! ATCTTGATTCTCGTTTGGTG+TGG - Chr6:17800833-17800852 MsG0680031306.01.T01:CDS 40.0%
! CTGCGTCTAGAGTATAATTC+TGG - Chr6:17800286-17800305 MsG0680031306.01.T01:CDS 40.0%
! GGTCAAATTTGATTGGCCAA+AGG - Chr6:17800243-17800262 MsG0680031306.01.T01:CDS 40.0%
! GTTTGGAAAGTGTAATCCGT+TGG + Chr6:17799869-17799888 None:intergenic 40.0%
ACTCGTTCTTGGAGCCAATA+TGG - Chr6:17799892-17799911 MsG0680031306.01.T01:CDS 45.0%
ATCCACGCATCTCTCCATAT+TGG + Chr6:17799909-17799928 None:intergenic 45.0%
CCAGAATCAAAAGGACTAGG+TGG + Chr6:17800424-17800443 None:intergenic 45.0%
CTCCAAGAACGAGTTTGGTT+TGG + Chr6:17799886-17799905 None:intergenic 45.0%
CTGGTGAGAGGTTTGAGAAT+AGG - Chr6:17800221-17800240 MsG0680031306.01.T01:CDS 45.0%
GGTCCAGAATCAAAAGGACT+AGG + Chr6:17800427-17800446 None:intergenic 45.0%
GGTCGTTCTAACTCAACCTT+TGG + Chr6:17800262-17800281 None:intergenic 45.0%
TATGGAGAGATGCGTGGATT+TGG - Chr6:17799910-17799929 MsG0680031306.01.T01:CDS 45.0%
TCACTTGGAATGGACAGATC+AGG + Chr6:17800357-17800376 None:intergenic 45.0%
TCCAATAAACCACCGTTCCA+AGG + Chr6:17800175-17800194 None:intergenic 45.0%
TCCTTGGAACGGTGGTTTAT+TGG - Chr6:17800171-17800190 MsG0680031306.01.T01:CDS 45.0%
! ACATTTTGTCCTTGGAACGG+TGG - Chr6:17800163-17800182 MsG0680031306.01.T01:CDS 45.0%
! ATTGGCTCCAAGAACGAGTT+TGG + Chr6:17799891-17799910 None:intergenic 45.0%
! GGATATGGTAGTGTTGCTCA+TGG - Chr6:17800322-17800341 MsG0680031306.01.T01:CDS 45.0%
!! CCACCTAGTCCTTTTGATTC+TGG - Chr6:17800421-17800440 MsG0680031306.01.T01:CDS 45.0%
!! TGTATGCTGTTTCTGGTGAG+AGG - Chr6:17800209-17800228 MsG0680031306.01.T01:CDS 45.0%
AGCCAATATGGAGAGATGCG+TGG - Chr6:17799904-17799923 MsG0680031306.01.T01:CDS 50.0%
GTCATTTGACTGGAGCGATG+AGG - Chr6:17800765-17800784 MsG0680031306.01.T01:CDS 50.0%
GTGACCTTGATGTGCTACCA+GGG - Chr6:17800545-17800564 MsG0680031306.01.T01:CDS 50.0%
TGTGACCTTGATGTGCTACC+AGG - Chr6:17800544-17800563 MsG0680031306.01.T01:CDS 50.0%
TTGTCCCTGGTAGCACATCA+AGG + Chr6:17800552-17800571 None:intergenic 50.0%
! ACATGGATGGACTCTCTCAC+TGG - Chr6:17800701-17800720 MsG0680031306.01.T01:CDS 50.0%
!! CTGAGGGTAAGGGTGTTGTA+AGG + Chr6:17800795-17800814 None:intergenic 50.0%
!! TCGTTTGGTGTGGAGTCACT+TGG - Chr6:17800843-17800862 MsG0680031306.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr6 gene 17799854 17800915 17799854 ID=MsG0680031306.01;Name=MsG0680031306.01
Chr6 mRNA 17799854 17800915 17799854 ID=MsG0680031306.01.T01;Parent=MsG0680031306.01;Name=MsG0680031306.01.T01;_AED=0.16;_eAED=0.16;_QI=0|-1|0|1|-1|1|1|0|353
Chr6 exon 17799854 17800915 17799854 ID=MsG0680031306.01.T01:exon:8092;Parent=MsG0680031306.01.T01
Chr6 CDS 17799854 17800915 17799854 ID=MsG0680031306.01.T01:cds;Parent=MsG0680031306.01.T01
Gene Sequence

>MsG0680031306.01.T01

ATGATATTTACTCCAACGGATTACACTTTCCAAACCAAACTCGTTCTTGGAGCCAATATGGAGAGATGCGTGGATTTGGCATCGACAGATGAGAATGCAAAGGTAAGAAGGAGACAATCACATATACCTCAAGATCTTTTCTTCTCTATTCTCTCTAAATTGCCTATTAAATCTTTGAAGCGATTTGGATGTGTAAGTAAACCATGGTCGCTCTTATTTGATAACCCTTATTTTATGACTATGTATCGCAACAACTTCTTAACAAAGGATCATTCTTATTATGATGATACATCTCTATTCATACATCAAACATTTTGTCCTTGGAACGGTGGTTTATTGGATAAAAAATTTGAGTTGTATGCTGTTTCTGGTGAGAGGTTTGAGAATAGGGTCAAATTTGATTGGCCAAAGGTTGAGTTAGAACGACCATATCTGCGTCTAGAGTATAATTCTGGTTTTGATATTTTAGGATATGGTAGTGTTGCTCATGGAACTCTTTGCCTGATCTGTCCATTCCAAGTGAATATTGTATTATGGAATCCATCTACCGAAGATTTTAAGGTTATTCCACCTAGTCCTTTTGATTCTGGACCTTATTGGGAAGCTTCTGTTTGTCATCAAGGATTTGGTTATGATGATATTAGAGACGATTATAAAGTGATGCGCTATATATATTTAACAAAAACTAATTGTGACCTTGATGTGCTACCAGGGACAAATATATCTTTCAACTCCTCGATATGGGAGATATATAGTCTAAGAAGTAACTCTTGGAGAAAATTTGACGTCGATATGCATCATGAGCTTATGGATGATGAACATTTGTACATGGATGATGAACAATTGTACATGGATGGACTCTCTCACTGGATGTGTAGTAGAAGTAAAAAACGTAATGAAAAATATTTGTTGTCATTTGACTGGAGCGATGAGGTTTTCCTTACAACACCCTTACCCTCAGACATGAATGATACTTTCAATCTTGATTCTCGTTTGGTGTGGAGTCACTTGGTGTTATTAAATGGTTCTATTTCTTTGATCTTTGTTGGAACATGTGTGTGA
Protein sequence

>MsG0680031306.01.T01

MIFTPTDYTFQTKLVLGANMERCVDLASTDENAKVRRRQSHIPQDLFFSILSKLPIKSLKRFGCVSKPWSLLFDNPYFMTMYRNNFLTKDHSYYDDTSLFIHQTFCPWNGGLLDKKFELYAVSGERFENRVKFDWPKVELERPYLRLEYNSGFDILGYGSVAHGTLCLICPFQVNIVLWNPSTEDFKVIPPSPFDSGPYWEASVCHQGFGYDDIRDDYKVMRYIYLTKTNCDLDVLPGTNISFNSSIWEIYSLRSNSWRKFDVDMHHELMDDEHLYMDDEQLYMDGLSHWMCSRSKKRNEKYLLSFDWSDEVFLTTPLPSDMNDTFNLDSRLVWSHLVLLNGSISLIFVGTCV*