AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035082.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035082.01.T01 MTR_0129s0070 49.429 350 156 10 1 337 56 397 4.41e-100 301
MsG0680035082.01.T01 MTR_0129s0120 48.696 345 156 10 1 332 56 392 1.01e-93 285
MsG0680035082.01.T01 MTR_4g117550 46.439 351 142 10 1 337 56 374 1.24e-92 281
MsG0680035082.01.T01 MTR_0129s0050 46.995 366 154 12 1 336 53 408 2.40e-89 274
MsG0680035082.01.T01 MTR_8g063440 44.798 346 170 10 1 337 51 384 9.58e-89 271
MsG0680035082.01.T01 MTR_2g038680 44.595 370 156 12 1 335 55 410 1.59e-86 266
MsG0680035082.01.T01 MTR_6g079770 44.022 368 164 11 1 334 56 415 1.00e-83 259
MsG0680035082.01.T01 MTR_6g090180 43.855 358 166 11 1 332 86 434 7.89e-83 258
MsG0680035082.01.T01 MTR_5g097330 42.090 354 174 11 1 332 55 399 3.15e-81 253
MsG0680035082.01.T01 MTR_6g086825 42.582 364 171 13 1 337 109 461 3.15e-80 252
MsG0680035082.01.T01 MTR_4g102410 43.944 355 172 13 1 334 100 448 5.90e-79 248
MsG0680035082.01.T01 MTR_4g028510 42.618 359 169 10 1 335 56 401 5.65e-77 243
MsG0680035082.01.T01 MTR_4g028680 42.577 357 175 11 1 337 55 401 9.64e-77 241
MsG0680035082.01.T01 MTR_0054s0100 41.096 365 176 12 1 337 56 409 1.96e-76 241
MsG0680035082.01.T01 MTR_4g130330 43.293 328 154 9 31 336 74 391 2.08e-76 240
MsG0680035082.01.T01 MTR_8g041270 42.359 373 162 12 2 337 51 407 3.85e-76 239
MsG0680035082.01.T01 MTR_2g104340 42.105 361 171 11 2 337 50 397 4.44e-76 239
MsG0680035082.01.T01 MTR_7g111700 43.210 324 137 8 30 337 74 366 4.57e-76 238
MsG0680035082.01.T01 MTR_0054s0090 41.096 365 174 13 1 337 56 407 7.11e-76 239
MsG0680035082.01.T01 MTR_2g021800 41.935 372 172 13 1 337 55 417 1.48e-74 236
MsG0680035082.01.T01 MTR_5g097350 41.880 351 163 12 1 332 55 383 3.30e-74 234
MsG0680035082.01.T01 MTR_2g021770 39.071 366 186 10 1 337 51 408 1.63e-73 233
MsG0680035082.01.T01 MTR_6g079810 41.389 360 178 12 1 334 109 461 2.62e-73 234
MsG0680035082.01.T01 MTR_0134s0060 39.459 370 182 10 2 337 52 413 4.62e-73 232
MsG0680035082.01.T01 MTR_2g022000 41.761 352 157 12 1 315 55 395 6.06e-73 232
MsG0680035082.01.T01 MTR_5g096630 38.753 369 180 12 1 335 57 413 1.43e-72 231
MsG0680035082.01.T01 MTR_4g028660 42.493 353 170 14 1 332 55 395 2.27e-72 229
MsG0680035082.01.T01 MTR_4g027450 42.493 353 170 14 1 332 55 395 2.27e-72 229
MsG0680035082.01.T01 MTR_3g011120 42.687 335 164 14 2 326 54 370 2.30e-72 229
MsG0680035082.01.T01 MTR_7g069550 42.614 352 163 15 1 327 53 390 2.65e-72 229
MsG0680035082.01.T01 MTR_5g097220 40.909 374 158 15 1 332 52 404 2.71e-72 230
MsG0680035082.01.T01 MTR_6g080680 41.127 355 161 12 1 330 58 389 4.32e-71 226
MsG0680035082.01.T01 MTR_8g467670 42.089 316 162 10 28 338 82 381 5.24e-71 226
MsG0680035082.01.T01 MTR_8g464770 41.593 339 176 12 2 332 52 376 1.47e-70 224
MsG0680035082.01.T01 MTR_7g017040 39.945 363 172 11 1 336 55 398 1.54e-70 225
MsG0680035082.01.T01 MTR_7g017130 40.057 352 186 9 1 337 57 398 2.02e-70 224
MsG0680035082.01.T01 MTR_2g066170 41.265 332 176 11 14 337 67 387 4.47e-70 223
MsG0680035082.01.T01 MTR_8g012090 42.188 320 131 11 1 306 55 334 5.30e-70 221
MsG0680035082.01.T01 MTR_1g007450 41.593 339 163 8 1 317 51 376 1.13e-69 223
MsG0680035082.01.T01 MTR_4g014370 39.037 374 174 15 1 335 50 408 1.55e-69 223
MsG0680035082.01.T01 MTR_7g017170 39.835 364 182 10 1 337 57 410 2.36e-69 222
MsG0680035082.01.T01 MTR_2g104280 40.220 363 180 11 2 335 50 404 7.12e-69 221
MsG0680035082.01.T01 MTR_6g078010 40.857 350 166 11 1 331 3 330 7.45e-69 219
MsG0680035082.01.T01 MTR_6g079790 43.963 323 139 12 43 337 2 310 7.82e-69 218
MsG0680035082.01.T01 MTR_4g007940 41.486 323 153 13 16 315 11 320 1.76e-68 218
MsG0680035082.01.T01 MTR_2g021950 39.601 351 181 12 1 332 55 393 2.23e-68 219
MsG0680035082.01.T01 MTR_5g037680 40.169 356 165 15 1 330 47 380 2.89e-68 221
MsG0680035082.01.T01 MTR_6g478230 39.510 367 168 12 1 337 66 408 1.41e-67 218
MsG0680035082.01.T01 MTR_5g096910 40.312 320 160 11 31 335 89 392 1.08e-66 215
MsG0680035082.01.T01 MTR_8g033140 37.270 381 176 12 1 337 58 419 3.85e-66 214
MsG0680035082.01.T01 MTR_1g007410 41.714 350 168 14 1 327 61 397 6.32e-66 213
MsG0680035082.01.T01 MTR_4g028750 41.801 311 152 11 28 321 84 382 1.47e-65 212
MsG0680035082.01.T01 MTR_5g097680 39.267 382 174 15 1 335 56 426 2.26e-65 213
MsG0680035082.01.T01 MTR_2g066200 38.418 354 186 14 1 337 51 389 3.64e-65 211
MsG0680035082.01.T01 MTR_5g064700 39.314 379 176 17 1 336 1 368 4.76e-65 210
MsG0680035082.01.T01 MTR_5g074800 40.395 354 165 14 1 327 1 335 4.97e-65 209
MsG0680035082.01.T01 MTR_6g461660 39.828 349 183 9 1 332 58 396 5.50e-65 211
MsG0680035082.01.T01 MTR_5g022850 39.877 326 165 10 31 337 41 354 4.74e-63 204
MsG0680035082.01.T01 MTR_8g039500 41.060 302 151 8 12 306 65 346 3.33e-62 202
MsG0680035082.01.T01 MTR_2g064950 38.592 355 168 15 1 327 49 381 1.73e-61 201
MsG0680035082.01.T01 MTR_4g055820 41.720 314 151 10 2 292 54 358 3.86e-61 199
MsG0680035082.01.T01 MTR_0831s0010 38.636 352 181 14 1 336 89 421 4.12e-61 201
MsG0680035082.01.T01 MTR_8g064890 37.216 352 189 12 1 337 55 389 5.41e-61 200
MsG0680035082.01.T01 MTR_5g069390 39.335 361 182 16 1 334 65 415 7.94e-61 202
MsG0680035082.01.T01 MTR_6g090250 37.931 377 181 11 1 331 50 419 9.26e-61 200
MsG0680035082.01.T01 MTR_2g034700 40.462 346 160 14 1 329 53 369 9.50e-61 199
MsG0680035082.01.T01 MTR_8g046310 41.499 347 159 17 1 331 55 373 1.38e-60 199
MsG0680035082.01.T01 MTR_5g059430 36.267 375 183 16 1 334 65 424 2.90e-60 199
MsG0680035082.01.T01 MTR_6g087620 39.935 308 134 10 36 326 6 279 3.09e-60 194
MsG0680035082.01.T01 MTR_7g079640 38.720 328 163 11 1 315 52 354 4.74e-60 197
MsG0680035082.01.T01 MTR_1g007430 40.850 306 154 8 1 295 35 324 7.26e-60 195
MsG0680035082.01.T01 MTR_2g065860 38.462 338 183 11 12 337 65 389 7.98e-60 197
MsG0680035082.01.T01 MTR_5g076490 41.209 364 156 13 1 336 3 336 1.36e-59 195
MsG0680035082.01.T01 MTR_1g053510 38.674 362 177 12 1 331 54 401 2.68e-59 196
MsG0680035082.01.T01 MTR_2g066150 38.872 337 155 14 12 337 65 361 4.86e-59 194
MsG0680035082.01.T01 MTR_5g086590 47.436 234 104 7 80 309 13 231 1.28e-58 189
MsG0680035082.01.T01 MTR_5g091140 40.708 339 147 15 1 315 87 395 4.34e-58 193
MsG0680035082.01.T01 MTR_5g092860 38.527 353 151 14 1 322 41 358 1.19e-57 191
MsG0680035082.01.T01 MTR_2g065840 36.723 354 191 13 1 336 51 389 2.81e-57 190
MsG0680035082.01.T01 MTR_8g033090 37.736 371 183 13 1 335 53 411 7.61e-57 190
MsG0680035082.01.T01 MTR_5g072060 40.419 334 146 14 1 312 45 347 2.53e-56 193
MsG0680035082.01.T01 MTR_7g023800 34.957 349 162 9 1 329 1 304 3.67e-56 185
MsG0680035082.01.T01 MTR_5g043605 38.730 315 157 12 32 333 45 336 1.28e-55 186
MsG0680035082.01.T01 MTR_8g013000 39.583 336 166 15 1 314 49 369 1.68e-55 186
MsG0680035082.01.T01 MTR_1g050312 40.789 304 136 13 23 315 3 273 1.68e-55 183
MsG0680035082.01.T01 MTR_8g036970 37.952 332 123 13 1 315 37 302 3.33e-55 184
MsG0680035082.01.T01 MTR_6g004450 44.578 249 106 8 105 338 40 271 4.56e-55 181
MsG0680035082.01.T01 MTR_5g068490 39.735 302 147 12 40 337 8 278 1.56e-54 180
MsG0680035082.01.T01 MTR_8g046200 35.079 382 180 13 2 331 52 417 6.83e-54 182
MsG0680035082.01.T01 MTR_8g032980 35.219 389 179 16 1 337 55 422 1.20e-52 179
MsG0680035082.01.T01 MTR_5g092850 37.500 328 159 12 35 332 87 398 3.64e-52 177
MsG0680035082.01.T01 MTR_8g033110 36.483 381 177 18 1 335 53 414 1.38e-51 176
MsG0680035082.01.T01 MTR_8g046220 35.389 373 172 13 1 331 56 401 6.57e-51 174
MsG0680035082.01.T01 MTR_5g024260 39.589 341 167 15 1 317 61 386 8.17e-51 174
MsG0680035082.01.T01 MTR_8g063330 37.864 309 162 13 1 292 53 348 8.94e-51 176
MsG0680035082.01.T01 MTR_2g021920 42.149 242 108 8 114 332 37 269 9.26e-51 170
MsG0680035082.01.T01 MTR_6g090260 46.377 207 90 6 136 331 63 259 5.93e-50 167
MsG0680035082.01.T01 MTR_8g018110 34.341 364 190 11 1 335 53 396 2.58e-49 170
MsG0680035082.01.T01 MTR_5g023970 47.000 200 90 7 145 337 7 197 5.20e-49 163
MsG0680035082.01.T01 MTR_5g097850 46.809 188 85 4 157 335 63 244 6.08e-49 164
MsG0680035082.01.T01 MTR_5g074850 35.277 343 176 14 1 314 76 401 1.94e-48 174
MsG0680035082.01.T01 MTR_1g050308 35.714 336 134 14 11 337 86 348 1.92e-47 164
MsG0680035082.01.T01 MTR_7g016930 38.065 310 148 10 1 297 55 333 4.15e-47 162
MsG0680035082.01.T01 MTR_8g033000 34.656 378 188 14 1 336 3 363 4.79e-47 163
MsG0680035082.01.T01 MTR_4g016000 44.550 211 87 9 85 287 2 190 8.82e-46 155
MsG0680035082.01.T01 MTR_8g064790 31.085 341 175 7 1 337 54 338 5.81e-45 157
MsG0680035082.01.T01 MTR_5g075370 34.286 315 158 14 33 314 79 377 2.35e-44 162
MsG0680035082.01.T01 MTR_5g098950 41.395 215 95 4 82 295 90 274 2.62e-44 153
MsG0680035082.01.T01 MTR_8g063370 32.558 344 194 13 1 328 63 384 8.10e-43 152
MsG0680035082.01.T01 MTR_1g098180 33.611 360 166 15 1 337 58 367 4.25e-42 150
MsG0680035082.01.T01 MTR_3g018750 39.837 246 110 10 114 332 3 237 1.56e-41 145
MsG0680035082.01.T01 MTR_8g035690 39.852 271 117 11 102 335 41 302 2.04e-41 146
MsG0680035082.01.T01 MTR_8g063350 34.796 319 160 14 31 327 45 337 5.62e-41 146
MsG0680035082.01.T01 MTR_7g019250 41.921 229 104 7 94 314 206 413 1.18e-40 148
MsG0680035082.01.T01 MTR_4g014370 40.000 200 99 5 146 335 148 336 1.25e-40 145
MsG0680035082.01.T01 MTR_7g104710 37.410 278 119 10 1 259 56 297 3.37e-36 133
MsG0680035082.01.T01 MTR_8g063310 32.500 320 173 14 31 327 89 388 3.38e-36 135
MsG0680035082.01.T01 MTR_6g009350 41.294 201 91 8 114 300 8 195 4.21e-36 129
MsG0680035082.01.T01 MTR_5g066420 47.518 141 60 3 183 314 2 137 1.83e-35 126
MsG0680035082.01.T01 MTR_6g079730 49.275 138 64 2 195 331 13 145 4.61e-33 120
MsG0680035082.01.T01 MTR_5g096920 44.231 156 76 5 183 334 2 150 7.82e-33 119
MsG0680035082.01.T01 MTR_5g097540 42.373 177 83 7 151 318 96 262 3.24e-32 122
MsG0680035082.01.T01 MTR_1g047080 31.805 349 159 16 1 337 3 284 1.39e-31 120
MsG0680035082.01.T01 MTR_3g030620 46.154 169 72 8 178 334 19 180 1.55e-31 117
MsG0680035082.01.T01 MTR_2g104260 36.681 229 118 8 2 206 50 275 2.38e-31 119
MsG0680035082.01.T01 MTR_5g096900 35.172 290 131 13 1 260 51 313 2.93e-31 120
MsG0680035082.01.T01 MTR_7g101200 37.054 224 99 9 78 292 36 226 3.88e-30 115
MsG0680035082.01.T01 MTR_5g097820 35.747 221 108 11 1 200 62 269 8.27e-29 112
MsG0680035082.01.T01 MTR_6g070880 38.660 194 94 8 1 173 71 260 2.59e-28 110
MsG0680035082.01.T01 MTR_6g071420 38.660 194 94 8 1 173 71 260 2.59e-28 110
MsG0680035082.01.T01 MTR_7g017150 39.901 203 99 7 147 337 77 268 5.62e-28 110
MsG0680035082.01.T01 MTR_7g017120 39.409 203 100 7 147 337 76 267 2.50e-27 108
MsG0680035082.01.T01 MTR_5g036250 50.000 104 50 1 235 338 12 113 4.28e-27 105
MsG0680035082.01.T01 MTR_5g094350 37.198 207 86 8 30 212 85 271 4.67e-27 108
MsG0680035082.01.T01 MTR_1g076810 37.824 193 102 8 1 183 57 241 5.77e-26 108
MsG0680035082.01.T01 MTR_5g091150 33.484 221 84 9 43 238 2 184 1.70e-24 99.4
MsG0680035082.01.T01 MTR_1g115205 39.412 170 73 6 32 189 85 236 3.13e-24 99.8
MsG0680035082.01.T01 MTR_5g097660 43.697 119 60 3 216 332 3 116 3.49e-24 95.9
MsG0680035082.01.T01 MTR_2g022020 40.164 122 67 2 211 332 1 116 6.79e-23 92.4
MsG0680035082.01.T01 MTR_5g086780 37.705 122 71 1 192 313 112 228 1.85e-22 94.4
MsG0680035082.01.T01 MTR_5g037190 32.579 221 107 6 1 203 55 251 2.79e-20 89.0
MsG0680035082.01.T01 MTR_3g047540 55.263 76 31 2 150 223 4 78 3.19e-19 81.6
MsG0680035082.01.T01 MTR_5g096050 41.739 115 45 4 183 281 2 110 1.27e-17 78.2
MsG0680035082.01.T01 MTR_1g050305 48.113 106 43 4 218 315 2 103 1.36e-17 79.0
MsG0680035082.01.T01 MTR_6g465360 34.247 146 79 5 1 132 101 243 1.66e-17 82.0
MsG0680035082.01.T01 MTR_3g097190 64.815 54 19 0 257 310 28 81 2.32e-17 79.3
MsG0680035082.01.T01 MTR_8g073570 38.889 126 72 5 1 125 49 170 2.93e-17 78.6
MsG0680035082.01.T01 MTR_4g094400 44.444 117 42 4 174 272 2 113 2.62e-16 74.7
MsG0680035082.01.T01 MTR_1g068835 24.648 284 174 13 43 309 74 334 4.58e-16 79.0
MsG0680035082.01.T01 MTR_2g026010 26.087 276 162 11 81 335 105 359 7.16e-16 78.2
MsG0680035082.01.T01 MTR_0667s0010 25.833 240 146 10 50 272 82 306 1.95e-15 77.0
MsG0680035082.01.T01 MTR_2g028110 26.354 277 171 13 50 310 78 337 3.48e-15 76.3
MsG0680035082.01.T01 MTR_2g028160 29.412 204 118 8 80 272 129 317 2.25e-14 73.9
MsG0680035082.01.T01 MTR_0129s0110 46.250 80 23 2 16 95 1 60 2.65e-14 68.9
MsG0680035082.01.T01 MTR_0064s0160 27.481 262 156 9 85 324 168 417 2.83e-14 73.9
MsG0680035082.01.T01 MTR_2g425980 26.199 271 166 12 80 331 121 376 3.72e-14 73.2
MsG0680035082.01.T01 MTR_2g028130 26.415 212 131 9 80 282 129 324 1.07e-13 72.0
MsG0680035082.01.T01 MTR_5g097730 68.421 38 12 0 259 296 15 52 7.23e-13 63.2
MsG0680035082.01.T01 MTR_5g048160 26.562 256 154 10 82 315 124 367 1.75e-12 68.2
MsG0680035082.01.T01 MTR_2g031640 25.092 271 160 14 59 307 87 336 1.78e-12 68.2
MsG0680035082.01.T01 MTR_2g021050 28.311 219 122 11 111 310 128 330 1.80e-12 68.2
MsG0680035082.01.T01 MTR_2g426070 26.667 255 138 15 80 310 128 357 2.12e-12 67.8
MsG0680035082.01.T01 MTR_2g024100 24.583 240 151 6 50 272 84 310 2.15e-12 67.8
MsG0680035082.01.T01 MTR_5g049560 34.351 131 66 5 25 142 35 158 4.41e-12 65.9
MsG0680035082.01.T01 MTR_2g031680 26.073 303 172 18 54 335 83 354 1.02e-11 65.9
MsG0680035082.01.T01 MTR_4g023080 25.185 270 158 9 76 334 113 349 1.95e-11 64.7
MsG0680035082.01.T01 MTR_2g021155 27.755 245 146 12 70 309 136 354 2.48e-11 65.5
MsG0680035082.01.T01 MTR_2g426090 25.632 277 158 15 80 335 123 372 4.42e-11 63.9
MsG0680035082.01.T01 MTR_3g036335 24.335 263 158 12 44 300 123 350 4.93e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035082.01.T01 AT4G12560 24.806 258 158 9 81 315 108 352 1.84e-14 74.3
MsG0680035082.01.T01 AT4G12560 24.806 258 158 9 81 315 108 352 1.84e-14 74.3
MsG0680035082.01.T01 AT4G12560 24.806 258 158 9 81 315 108 352 1.84e-14 74.3

Find 75 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTCCGAAAGTTGTATTT+TGG 0.152839 6:-98604124 MsG0680035082.01.T01:CDS
AACACCCCTACCCTTATATT+TGG 0.164053 6:-98603730 MsG0680035082.01.T01:CDS
AGATGCTTTGCGGTGTTAAA+TGG 0.182794 6:-98603678 MsG0680035082.01.T01:CDS
TGCATTTAATGTATCACTTT+TGG 0.198852 6:-98603604 MsG0680035082.01.T01:CDS
ATAATGATCATCCAAATATA+AGG 0.250011 6:+98603719 None:intergenic
TATTGAAATACTTCTTTATA+AGG 0.254498 6:-98603397 MsG0680035082.01.T01:CDS
TACCAAGCTTCTCAATCATT+TGG 0.277111 6:+98603436 None:intergenic
ATAGCTTAAGAAGTAATTCT+TGG 0.288276 6:-98603890 MsG0680035082.01.T01:CDS
TCCCACCACACGACTCCTTT+TGG 0.293440 6:-98603920 MsG0680035082.01.T01:CDS
ACATGAATGATCGACGTTAT+TGG 0.294156 6:+98604331 None:intergenic
AAAGAAGTATTTCAATATAA+AGG 0.299879 6:+98603403 None:intergenic
ATGTCTCACATTAGACTTAG+AGG 0.308944 6:-98604312 MsG0680035082.01.T01:CDS
CCTACTGGTTATCTTACTTC+AGG 0.318949 6:-98603846 MsG0680035082.01.T01:CDS
CGTTGAATTCCTTAGTAGTT+AGG 0.322190 6:+98604099 None:intergenic
CCTTGTATTGACCATCCTAT+TGG 0.341729 6:-98603528 MsG0680035082.01.T01:CDS
AGAATAAAGTCAAATTAGAT+TGG 0.371258 6:-98604235 MsG0680035082.01.T01:CDS
AGGTTCCAAAATACAACTTT+CGG 0.371388 6:+98604119 None:intergenic
ACCTTATGGTCATTGCTAAT+AGG 0.377981 6:+98603996 None:intergenic
CCTTTGTTTCCTGCTCCAAT+AGG 0.388585 6:+98603513 None:intergenic
ATGTACATAGCTTGTTTCAT+AGG 0.397178 6:+98603635 None:intergenic
ACCAAGCTTCTCAATCATTT+GGG 0.397734 6:+98603437 None:intergenic
CTGCATGTCGAATAACCTTA+TGG 0.403711 6:+98603982 None:intergenic
CAAAAGATGATGAACTTGTT+CGG 0.404344 6:-98603473 MsG0680035082.01.T01:CDS
GACCAAGGTATTAGAATGTT+GGG 0.434737 6:-98604191 MsG0680035082.01.T01:CDS
GATGCTTTGCGGTGTTAAAT+GGG 0.459635 6:-98603677 MsG0680035082.01.T01:CDS
TCCTATTAGCAATGACCATA+AGG 0.471847 6:-98603997 MsG0680035082.01.T01:CDS
AACCCAACATTCTAATACCT+TGG 0.482364 6:+98604189 None:intergenic
TGACCAAGGTATTAGAATGT+TGG 0.483267 6:-98604192 MsG0680035082.01.T01:CDS
AAGAAGGTGGCGTGAACTCA+AGG 0.486787 6:+98604060 None:intergenic
TAATGATCATCCAAATATAA+GGG 0.490125 6:+98603720 None:intergenic
ATCATCCAAATATAAGGGTA+GGG 0.491811 6:+98603725 None:intergenic
ATTGACCATCCTATTGGAGC+AGG 0.503407 6:-98603522 MsG0680035082.01.T01:CDS
TGTTTCCTGCTCCAATAGGA+TGG 0.505796 6:+98603517 None:intergenic
CCTTGGTCATCCTCGTGAAA+CGG 0.521395 6:+98604206 None:intergenic
ACACTCCATTCTTGTACACT+CGG 0.526608 6:+98603820 None:intergenic
TTAGTCCTCCGATCGGGAGA+AGG 0.528354 6:+98603370 None:intergenic
ACAAGAATGGAGTGTGTCAT+TGG 0.531939 6:-98603812 MsG0680035082.01.T01:CDS
GGTTCCCGAGTGTACAAGAA+TGG 0.532291 6:-98603825 MsG0680035082.01.T01:CDS
CCAATAGGATGGTCAATACA+AGG 0.535198 6:+98603528 None:intergenic
GATCATCCAAATATAAGGGT+AGG 0.535975 6:+98603724 None:intergenic
AGTGATACATTAAATGCAGT+TGG 0.542475 6:+98603609 None:intergenic
TAAGCTATATACCTCCCAAA+AGG 0.546670 6:+98603905 None:intergenic
AACTTGGTGTGAAAGAATCA+TGG 0.547068 6:-98603578 MsG0680035082.01.T01:CDS
ACCCAAATGATTGAGAAGCT+TGG 0.547419 6:-98603438 MsG0680035082.01.T01:CDS
ATTAGTGGATAGATGCTTTG+CGG 0.547810 6:-98603688 MsG0680035082.01.T01:CDS
AATGTGAAAACAAGGCATCA+TGG 0.553369 6:+98604273 None:intergenic
TCCCAAAAGGAGTCGTGTGG+TGG 0.562279 6:+98603918 None:intergenic
CTTGATGTAGATATGCCTAC+TGG 0.563644 6:-98603861 MsG0680035082.01.T01:CDS
TCATCCTCGTGAAACGGAGG+TGG 0.579669 6:+98604212 None:intergenic
CTCACCAGAATGTGAAAACA+AGG 0.579748 6:+98604265 None:intergenic
GACACTCTTTGTTTATACCA+AGG 0.582783 6:-98604152 MsG0680035082.01.T01:CDS
CCTATTGGAGCAGGAAACAA+AGG 0.583784 6:-98603513 MsG0680035082.01.T01:CDS
AGAAGGTGGCGTGAACTCAA+GGG 0.586840 6:+98604061 None:intergenic
CCCAAAAGGAGTCGTGTGGT+GGG 0.587442 6:+98603919 None:intergenic
AAGGAAGTCCTTCTCCCGAT+CGG 0.596639 6:-98603378 MsG0680035082.01.T01:CDS
GCATCATGGAGTTCGTAGGT+TGG 0.600474 6:+98604287 None:intergenic
TATTGGATATGAAATTGTTG+CGG 0.609249 6:+98604348 None:intergenic
TCATCCAAATATAAGGGTAG+GGG 0.611586 6:+98603726 None:intergenic
CACTCCATTCTTGTACACTC+GGG 0.617230 6:+98603821 None:intergenic
CAAGGCATCATGGAGTTCGT+AGG 0.620224 6:+98604283 None:intergenic
ACTCAAGGGGACTCGAAGGA+AGG 0.621265 6:+98604075 None:intergenic
AGAATGGAGTGTGTCATTGG+TGG 0.629907 6:-98603809 MsG0680035082.01.T01:CDS
AAAACAGTTTCATAAGAAGG+TGG 0.630883 6:+98604047 None:intergenic
GAAGGTGGCGTGAACTCAAG+GGG 0.634564 6:+98604062 None:intergenic
GAATGGAGTGTGTCATTGGT+GGG 0.635631 6:-98603808 MsG0680035082.01.T01:CDS
GTGAACTCAAGGGGACTCGA+AGG 0.638844 6:+98604071 None:intergenic
GAAGTCCTTCTCCCGATCGG+AGG 0.652052 6:-98603375 MsG0680035082.01.T01:CDS
TTGGCCACCTCCGTTTCACG+AGG 0.654456 6:-98604216 MsG0680035082.01.T01:CDS
GGATGGTCAATACAAGGCAA+CGG 0.654523 6:+98603534 None:intergenic
ATAGGTTGAGATACAAGCAA+CGG 0.668565 6:+98603653 None:intergenic
TGGTCATCCTCGTGAAACGG+AGG 0.668994 6:+98604209 None:intergenic
ACCTCCCAAAAGGAGTCGTG+TGG 0.672209 6:+98603915 None:intergenic
CCTGAAGTAAGATAACCAGT+AGG 0.695300 6:+98603846 None:intergenic
CCGTTTCACGAGGATGACCA+AGG 0.703535 6:-98604206 MsG0680035082.01.T01:CDS
TAAACAAAGAGTGTCGTCTG+CGG 0.706299 6:+98604160 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGAAATACTTCTTTATA+AGG - Chr6:98604315-98604334 MsG0680035082.01.T01:CDS 15.0%
!!! AAAGAAGTATTTCAATATAA+AGG + Chr6:98604312-98604331 None:intergenic 15.0%
!! AGAATAAAGTCAAATTAGAT+TGG - Chr6:98603477-98603496 MsG0680035082.01.T01:CDS 20.0%
!! ATAAAAACAGTTTCATAAGA+AGG + Chr6:98603671-98603690 None:intergenic 20.0%
!! ATAATGATCATCCAAATATA+AGG + Chr6:98603996-98604015 None:intergenic 20.0%
!! TAATGATCATCCAAATATAA+GGG + Chr6:98603995-98604014 None:intergenic 20.0%
!!! TCATTATGATTTTGAATTAG+TGG - Chr6:98604009-98604028 MsG0680035082.01.T01:CDS 20.0%
! ATAGCTTAAGAAGTAATTCT+TGG - Chr6:98603822-98603841 MsG0680035082.01.T01:CDS 25.0%
! TATTGGATATGAAATTGTTG+CGG + Chr6:98603367-98603386 None:intergenic 25.0%
!! AAATTCCGAAAGTTGTATTT+TGG - Chr6:98603588-98603607 MsG0680035082.01.T01:CDS 25.0%
!! TGCATTTAATGTATCACTTT+TGG - Chr6:98604108-98604127 MsG0680035082.01.T01:CDS 25.0%
!!! ACTGTTTTTATTAGCTTTCA+TGG - Chr6:98603680-98603699 MsG0680035082.01.T01:CDS 25.0%
!!! GCATTTAATGTATCACTTTT+GGG - Chr6:98604109-98604128 MsG0680035082.01.T01:CDS 25.0%
AAAACAGTTTCATAAGAAGG+TGG + Chr6:98603668-98603687 None:intergenic 30.0%
AGGTTCCAAAATACAACTTT+CGG + Chr6:98603596-98603615 None:intergenic 30.0%
AGTGATACATTAAATGCAGT+TGG + Chr6:98604106-98604125 None:intergenic 30.0%
ATCATCCAAATATAAGGGTA+GGG + Chr6:98603990-98604009 None:intergenic 30.0%
! CAAAAGATGATGAACTTGTT+CGG - Chr6:98604239-98604258 MsG0680035082.01.T01:CDS 30.0%
!! ATGTACATAGCTTGTTTCAT+AGG + Chr6:98604080-98604099 None:intergenic 30.0%
!!! ATTTTGGAACCTAACTACTA+AGG - Chr6:98603604-98603623 MsG0680035082.01.T01:CDS 30.0%
!!! CTTTCGGAATTTTAAAACCT+TGG + Chr6:98603580-98603599 None:intergenic 30.0%
AACCCAACATTCTAATACCT+TGG + Chr6:98603526-98603545 None:intergenic 35.0%
ACATGAATGATCGACGTTAT+TGG + Chr6:98603384-98603403 None:intergenic 35.0%
ACCAAGCTTCTCAATCATTT+GGG + Chr6:98604278-98604297 None:intergenic 35.0%
ACCTTATGGTCATTGCTAAT+AGG + Chr6:98603719-98603738 None:intergenic 35.0%
ATGTCTCACATTAGACTTAG+AGG - Chr6:98603400-98603419 MsG0680035082.01.T01:CDS 35.0%
CGTTGAATTCCTTAGTAGTT+AGG + Chr6:98603616-98603635 None:intergenic 35.0%
GATCATCCAAATATAAGGGT+AGG + Chr6:98603991-98604010 None:intergenic 35.0%
TAAGCTATATACCTCCCAAA+AGG + Chr6:98603810-98603829 None:intergenic 35.0%
TACCAAGCTTCTCAATCATT+TGG + Chr6:98604279-98604298 None:intergenic 35.0%
TCATCCAAATATAAGGGTAG+GGG + Chr6:98603989-98604008 None:intergenic 35.0%
TCCTATTAGCAATGACCATA+AGG - Chr6:98603715-98603734 MsG0680035082.01.T01:CDS 35.0%
! AATGTGAAAACAAGGCATCA+TGG + Chr6:98603442-98603461 None:intergenic 35.0%
! ATAGGTTGAGATACAAGCAA+CGG + Chr6:98604062-98604081 None:intergenic 35.0%
! ATTAGTGGATAGATGCTTTG+CGG - Chr6:98604024-98604043 MsG0680035082.01.T01:CDS 35.0%
! GACACTCTTTGTTTATACCA+AGG - Chr6:98603560-98603579 MsG0680035082.01.T01:CDS 35.0%
! GACCAAGGTATTAGAATGTT+GGG - Chr6:98603521-98603540 MsG0680035082.01.T01:CDS 35.0%
! TGACCAAGGTATTAGAATGT+TGG - Chr6:98603520-98603539 MsG0680035082.01.T01:CDS 35.0%
! TGGACGAAACTTTTTGTGTT+TGG - Chr6:98604154-98604173 MsG0680035082.01.T01:CDS 35.0%
!! AACTTGGTGTGAAAGAATCA+TGG - Chr6:98604134-98604153 MsG0680035082.01.T01:CDS 35.0%
!!! ACATTTTTTAGTCCTCCGAT+CGG + Chr6:98604352-98604371 None:intergenic 35.0%
AACACCCCTACCCTTATATT+TGG - Chr6:98603982-98604001 MsG0680035082.01.T01:CDS 40.0%
ACAAGAATGGAGTGTGTCAT+TGG - Chr6:98603900-98603919 MsG0680035082.01.T01:CDS 40.0%
ACACTCCATTCTTGTACACT+CGG + Chr6:98603895-98603914 None:intergenic 40.0%
ACCCAAATGATTGAGAAGCT+TGG - Chr6:98604274-98604293 MsG0680035082.01.T01:CDS 40.0%
CCAATAGGATGGTCAATACA+AGG + Chr6:98604187-98604206 None:intergenic 40.0%
CCTGAAGTAAGATAACCAGT+AGG + Chr6:98603869-98603888 None:intergenic 40.0%
CTCACCAGAATGTGAAAACA+AGG + Chr6:98603450-98603469 None:intergenic 40.0%
CTGCATGTCGAATAACCTTA+TGG + Chr6:98603733-98603752 None:intergenic 40.0%
CTTGATGTAGATATGCCTAC+TGG - Chr6:98603851-98603870 MsG0680035082.01.T01:CDS 40.0%
! AGATGCTTTGCGGTGTTAAA+TGG - Chr6:98604034-98604053 MsG0680035082.01.T01:CDS 40.0%
! CCTACTGGTTATCTTACTTC+AGG - Chr6:98603866-98603885 MsG0680035082.01.T01:CDS 40.0%
! CCTTGTATTGACCATCCTAT+TGG - Chr6:98604184-98604203 MsG0680035082.01.T01:CDS 40.0%
! GATGCTTTGCGGTGTTAAAT+GGG - Chr6:98604035-98604054 MsG0680035082.01.T01:CDS 40.0%
! TGTTTTCACATTCTGGTGAG+AGG - Chr6:98603450-98603469 MsG0680035082.01.T01:CDS 40.0%
!! GTATCACTTTTGGGTGAACT+TGG - Chr6:98604118-98604137 MsG0680035082.01.T01:CDS 40.0%
!! TAAACAAAGAGTGTCGTCTG+CGG + Chr6:98603555-98603574 None:intergenic 40.0%
!!! CATTTTTTAGTCCTCCGATC+GGG + Chr6:98604351-98604370 None:intergenic 40.0%
!!! CTGAGTTTTACTGACTCTGA+AGG - Chr6:98603761-98603780 MsG0680035082.01.T01:CDS 40.0%
!!! GATGCCTTGTTTTCACATTC+TGG - Chr6:98603443-98603462 MsG0680035082.01.T01:CDS 40.0%
ATTGACCATCCTATTGGAGC+AGG - Chr6:98604190-98604209 MsG0680035082.01.T01:CDS 45.0%
CACTCCATTCTTGTACACTC+GGG + Chr6:98603894-98603913 None:intergenic 45.0%
CCTATTGGAGCAGGAAACAA+AGG - Chr6:98604199-98604218 MsG0680035082.01.T01:CDS 45.0%
GGATGGTCAATACAAGGCAA+CGG + Chr6:98604181-98604200 None:intergenic 45.0%
TGTTTCCTGCTCCAATAGGA+TGG + Chr6:98604198-98604217 None:intergenic 45.0%
! AGAATGGAGTGTGTCATTGG+TGG - Chr6:98603903-98603922 MsG0680035082.01.T01:CDS 45.0%
! CCTTTGTTTCCTGCTCCAAT+AGG + Chr6:98604202-98604221 None:intergenic 45.0%
! GAATGGAGTGTGTCATTGGT+GGG - Chr6:98603904-98603923 MsG0680035082.01.T01:CDS 45.0%
AAGAAGGTGGCGTGAACTCA+AGG + Chr6:98603655-98603674 None:intergenic 50.0%
AAGGAAGTCCTTCTCCCGAT+CGG - Chr6:98604334-98604353 MsG0680035082.01.T01:CDS 50.0%
AGAAGGTGGCGTGAACTCAA+GGG + Chr6:98603654-98603673 None:intergenic 50.0%
CCTTGGTCATCCTCGTGAAA+CGG + Chr6:98603509-98603528 None:intergenic 50.0%
GCATCATGGAGTTCGTAGGT+TGG + Chr6:98603428-98603447 None:intergenic 50.0%
GGTTCCCGAGTGTACAAGAA+TGG - Chr6:98603887-98603906 MsG0680035082.01.T01:CDS 50.0%
! CAAGGCATCATGGAGTTCGT+AGG + Chr6:98603432-98603451 None:intergenic 50.0%
ACCTCCCAAAAGGAGTCGTG+TGG + Chr6:98603800-98603819 None:intergenic 55.0%
ACTCAAGGGGACTCGAAGGA+AGG + Chr6:98603640-98603659 None:intergenic 55.0%
CCCAAAAGGAGTCGTGTGGT+GGG + Chr6:98603796-98603815 None:intergenic 55.0%
CCGTTTCACGAGGATGACCA+AGG - Chr6:98603506-98603525 MsG0680035082.01.T01:CDS 55.0%
GAAGGTGGCGTGAACTCAAG+GGG + Chr6:98603653-98603672 None:intergenic 55.0%
GTGAACTCAAGGGGACTCGA+AGG + Chr6:98603644-98603663 None:intergenic 55.0%
TCATCCTCGTGAAACGGAGG+TGG + Chr6:98603503-98603522 None:intergenic 55.0%
TCCCAAAAGGAGTCGTGTGG+TGG + Chr6:98603797-98603816 None:intergenic 55.0%
TGGTCATCCTCGTGAAACGG+AGG + Chr6:98603506-98603525 None:intergenic 55.0%
TTAGTCCTCCGATCGGGAGA+AGG + Chr6:98604345-98604364 None:intergenic 55.0%
! ACCACACGACTCCTTTTGGG+AGG - Chr6:98603796-98603815 MsG0680035082.01.T01:CDS 55.0%
! CCCACCACACGACTCCTTTT+GGG - Chr6:98603793-98603812 MsG0680035082.01.T01:CDS 55.0%
! TCCCACCACACGACTCCTTT+TGG - Chr6:98603792-98603811 MsG0680035082.01.T01:CDS 55.0%
GAAGTCCTTCTCCCGATCGG+AGG - Chr6:98604337-98604356 MsG0680035082.01.T01:CDS 60.0%
TTGGCCACCTCCGTTTCACG+AGG - Chr6:98603496-98603515 MsG0680035082.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 98603359 98604375 98603359 ID=MsG0680035082.01;Name=MsG0680035082.01
Chr6 mRNA 98603359 98604375 98603359 ID=MsG0680035082.01.T01;Parent=MsG0680035082.01;Name=MsG0680035082.01.T01;_AED=0.98;_eAED=0.98;_QI=0|-1|0|1|-1|1|1|0|338
Chr6 exon 98603359 98604375 98603359 ID=MsG0680035082.01.T01:exon:14004;Parent=MsG0680035082.01.T01
Chr6 CDS 98603359 98604375 98603359 ID=MsG0680035082.01.T01:cds;Parent=MsG0680035082.01.T01
Gene Sequence

>MsG0680035082.01.T01

ATGTACCGCAACAATTTCATATCCAATAACGTCGATCATTCATGTCTCACATTAGACTTAGAGGAGCCAACCTACGAACTCCATGATGCCTTGTTTTCACATTCTGGTGAGAGGTTTGAGAATAAAGTCAAATTAGATTGGCCACCTCCGTTTCACGAGGATGACCAAGGTATTAGAATGTTGGGTTATGCTGCCGCAGACGACACTCTTTGTTTATACCAAGGTTTTAAAATTCCGAAAGTTGTATTTTGGAACCTAACTACTAAGGAATTCAACGTCCTTCCTTCGAGTCCCCTTGAGTTCACGCCACCTTCTTATGAAACTGTTTTTATTAGCTTTCATGGATTTGCTTATGATCCTATTAGCAATGACCATAAGGTTATTCGACATGCAGCGCATATTCTGAGTTTTACTGACTCTGAAGGTTGTTATTTCCCACCACACGACTCCTTTTGGGAGGTATATAGCTTAAGAAGTAATTCTTGGAGAAAACTTGATGTAGATATGCCTACTGGTTATCTTACTTCAGGTTCCCGAGTGTACAAGAATGGAGTGTGTCATTGGTGGGATGAACGTGAAGCTTGTTTAGTGTCATTTGACTTGAGCAAAGATGTGTTCTTTAGAACACCCCTACCCTTATATTTGGATGATCATTATGATTTTGAATTAGTGGATAGATGCTTTGCGGTGTTAAATGGGTCCGTTGCTTGTATCTCAACCTATGAAACAAGCTATGTACATACACCAACTGCATTTAATGTATCACTTTTGGGTGAACTTGGTGTGAAAGAATCATGGACGAAACTTTTTGTGTTTGGTCCGTTGCCTTGTATTGACCATCCTATTGGAGCAGGAAACAAAGGCGATATATTCTTTAGATCAAAAGATGATGAACTTGTTCGGTTTGATATGAGTACCCAAATGATTGAGAAGCTTGGTATTAAACATGTCCTTTATATTGAAATACTTCTTTATAAGGAAGTCCTTCTCCCGATCGGAGGACTAAAAAATGTATGA
Protein sequence

>MsG0680035082.01.T01

MYRNNFISNNVDHSCLTLDLEEPTYELHDALFSHSGERFENKVKLDWPPPFHEDDQGIRMLGYAAADDTLCLYQGFKIPKVVFWNLTTKEFNVLPSSPLEFTPPSYETVFISFHGFAYDPISNDHKVIRHAAHILSFTDSEGCYFPPHDSFWEVYSLRSNSWRKLDVDMPTGYLTSGSRVYKNGVCHWWDEREACLVSFDLSKDVFFRTPLPLYLDDHYDFELVDRCFAVLNGSVACISTYETSYVHTPTAFNVSLLGELGVKESWTKLFVFGPLPCIDHPIGAGNKGDIFFRSKDDELVRFDMSTQMIEKLGIKHVLYIEILLYKEVLLPIGGLKNV*