AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580030061.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580030061.01.T01 MTR_5g096910 76.190 399 49 5 1 361 1 391 0.0 577
MsG0580030061.01.T01 MTR_5g037680 45.195 385 163 11 13 357 4 380 1.69e-97 297
MsG0580030061.01.T01 MTR_5g069390 43.596 406 166 14 10 361 19 415 5.33e-91 282
MsG0580030061.01.T01 MTR_5g059430 42.439 410 174 12 10 361 19 424 2.75e-90 278
MsG0580030061.01.T01 MTR_8g012090 44.353 363 144 12 1 334 1 334 5.48e-86 263
MsG0580030061.01.T01 MTR_5g096920 85.526 152 20 1 214 363 1 152 7.13e-85 254
MsG0580030061.01.T01 MTR_7g017130 38.614 404 185 11 6 361 7 395 2.42e-81 254
MsG0580030061.01.T01 MTR_5g097330 40.587 409 172 13 7 359 6 399 2.11e-80 251
MsG0580030061.01.T01 MTR_0129s0070 39.372 414 176 13 1 360 1 393 3.37e-80 251
MsG0580030061.01.T01 MTR_5g097680 39.762 420 171 13 15 361 15 425 6.26e-80 251
MsG0580030061.01.T01 MTR_3g011120 39.846 389 177 11 3 353 1 370 1.19e-79 248
MsG0580030061.01.T01 MTR_7g017170 37.500 416 185 12 6 361 7 407 1.33e-78 247
MsG0580030061.01.T01 MTR_6g086825 39.151 424 172 13 3 361 56 458 1.84e-78 248
MsG0580030061.01.T01 MTR_4g028680 41.247 417 165 15 4 361 3 398 1.01e-77 244
MsG0580030061.01.T01 MTR_2g034700 40.103 389 169 12 8 356 5 369 1.24e-77 243
MsG0580030061.01.T01 MTR_4g130330 40.146 411 160 13 13 361 3 389 6.67e-77 242
MsG0580030061.01.T01 MTR_0129s0120 39.225 413 176 13 1 359 1 392 1.16e-76 241
MsG0580030061.01.T01 MTR_2g065840 36.585 410 181 11 7 361 2 387 4.01e-76 240
MsG0580030061.01.T01 MTR_8g063440 38.693 398 181 11 7 360 2 380 4.11e-75 237
MsG0580030061.01.T01 MTR_2g038680 36.817 421 184 13 6 361 6 409 6.36e-75 238
MsG0580030061.01.T01 MTR_2g065860 37.469 403 186 12 7 361 2 386 6.40e-75 237
MsG0580030061.01.T01 MTR_4g028510 39.618 419 171 16 4 361 3 400 2.53e-74 238
MsG0580030061.01.T01 MTR_2g066170 37.562 402 185 14 7 361 2 384 5.78e-74 234
MsG0580030061.01.T01 MTR_6g090180 38.186 419 184 11 1 359 31 434 1.11e-73 235
MsG0580030061.01.T01 MTR_0134s0060 38.642 427 172 14 7 361 2 410 1.75e-73 234
MsG0580030061.01.T01 MTR_0054s0090 39.243 423 169 16 4 361 5 404 3.54e-73 233
MsG0580030061.01.T01 MTR_2g022000 37.081 418 189 9 1 355 1 407 5.03e-73 233
MsG0580030061.01.T01 MTR_7g069550 39.547 397 169 12 14 355 11 391 6.58e-73 232
MsG0580030061.01.T01 MTR_0054s0100 38.642 427 175 16 1 361 1 406 2.93e-72 231
MsG0580030061.01.T01 MTR_8g464770 38.120 383 181 9 14 357 9 374 3.43e-72 229
MsG0580030061.01.T01 MTR_6g080680 37.871 404 177 12 6 357 8 389 6.95e-72 229
MsG0580030061.01.T01 MTR_4g117550 37.073 410 170 11 1 361 1 371 1.82e-71 228
MsG0580030061.01.T01 MTR_5g097350 38.830 376 171 11 26 359 25 383 1.84e-71 228
MsG0580030061.01.T01 MTR_0129s0050 36.471 425 178 14 7 361 4 406 2.05e-70 226
MsG0580030061.01.T01 MTR_0831s0010 38.155 401 167 13 13 360 46 418 4.64e-70 225
MsG0580030061.01.T01 MTR_8g039500 38.919 370 159 14 7 334 2 346 4.92e-70 223
MsG0580030061.01.T01 MTR_2g066200 37.313 402 188 15 7 361 2 386 6.83e-70 224
MsG0580030061.01.T01 MTR_6g079770 39.951 408 177 13 15 361 15 415 7.84e-70 224
MsG0580030061.01.T01 MTR_4g102410 38.350 412 185 13 6 361 50 448 1.71e-69 224
MsG0580030061.01.T01 MTR_5g097220 36.215 428 171 13 7 359 4 404 1.94e-69 223
MsG0580030061.01.T01 MTR_8g046310 37.437 398 187 12 4 362 3 377 1.07e-68 221
MsG0580030061.01.T01 MTR_5g096630 37.346 407 181 13 17 361 18 412 3.10e-68 220
MsG0580030061.01.T01 MTR_7g017040 37.750 400 177 12 15 360 14 395 3.39e-68 220
MsG0580030061.01.T01 MTR_2g021950 37.871 404 186 13 6 359 5 393 4.98e-68 219
MsG0580030061.01.T01 MTR_1g007450 36.364 385 173 10 17 347 12 378 5.26e-68 220
MsG0580030061.01.T01 MTR_2g021800 36.038 419 193 10 1 355 1 408 7.85e-68 219
MsG0580030061.01.T01 MTR_6g478230 38.235 408 182 12 6 361 16 405 1.75e-67 219
MsG0580030061.01.T01 MTR_6g461660 38.366 404 180 11 8 359 10 396 1.84e-67 218
MsG0580030061.01.T01 MTR_7g079640 38.889 360 169 11 16 342 12 353 4.94e-67 216
MsG0580030061.01.T01 MTR_5g097850 49.160 238 96 6 137 361 18 243 1.71e-66 211
MsG0580030061.01.T01 MTR_5g091140 39.730 370 184 13 7 346 38 398 5.33e-66 215
MsG0580030061.01.T01 MTR_2g021770 35.784 408 179 13 20 361 15 405 6.28e-66 214
MsG0580030061.01.T01 MTR_6g079810 38.626 422 184 16 1 361 54 461 6.61e-66 216
MsG0580030061.01.T01 MTR_4g028660 38.481 395 164 15 4 341 3 375 6.77e-66 214
MsG0580030061.01.T01 MTR_4g027450 38.481 395 164 15 4 341 3 375 6.77e-66 214
MsG0580030061.01.T01 MTR_5g096900 42.991 321 135 9 6 287 2 313 7.27e-66 211
MsG0580030061.01.T01 MTR_5g092850 35.849 424 168 14 12 363 11 402 4.86e-65 212
MsG0580030061.01.T01 MTR_2g104340 36.967 422 177 16 1 361 1 394 3.21e-63 207
MsG0580030061.01.T01 MTR_8g036970 40.426 329 168 11 21 346 2 305 4.20e-63 206
MsG0580030061.01.T01 MTR_4g028750 37.666 377 165 12 13 337 12 370 6.46e-63 206
MsG0580030061.01.T01 MTR_5g072060 38.965 367 166 12 12 340 1 347 8.75e-63 211
MsG0580030061.01.T01 MTR_1g053510 34.988 423 186 14 3 358 1 401 1.93e-62 205
MsG0580030061.01.T01 MTR_2g066150 36.649 382 190 14 7 361 2 358 8.56e-62 202
MsG0580030061.01.T01 MTR_8g041270 37.164 409 178 15 15 361 13 404 1.16e-61 203
MsG0580030061.01.T01 MTR_4g055820 37.265 373 157 12 7 320 4 358 1.28e-61 201
MsG0580030061.01.T01 MTR_8g064890 35.930 398 187 11 11 361 10 386 2.01e-61 202
MsG0580030061.01.T01 MTR_5g074850 36.207 406 168 12 6 345 24 404 2.06e-61 212
MsG0580030061.01.T01 MTR_1g007410 37.984 387 166 14 14 345 19 386 7.26e-61 201
MsG0580030061.01.T01 MTR_5g024260 34.596 396 188 13 1 342 5 383 1.15e-60 201
MsG0580030061.01.T01 MTR_2g104280 35.452 409 185 14 15 361 12 403 1.81e-59 197
MsG0580030061.01.T01 MTR_8g467670 34.409 372 187 12 11 345 8 359 1.37e-58 195
MsG0580030061.01.T01 MTR_8g064790 34.656 378 185 12 3 361 1 335 1.49e-57 190
MsG0580030061.01.T01 MTR_5g075370 35.821 402 164 14 13 345 4 380 2.31e-57 199
MsG0580030061.01.T01 MTR_7g111700 38.107 391 182 14 1 360 1 362 9.84e-57 189
MsG0580030061.01.T01 MTR_4g014370 33.333 408 204 9 14 361 8 407 3.54e-56 189
MsG0580030061.01.T01 MTR_4g014370 34.946 372 189 10 1 361 6 335 1.03e-55 186
MsG0580030061.01.T01 MTR_5g086590 43.644 236 94 5 146 346 6 237 1.76e-55 182
MsG0580030061.01.T01 MTR_8g032980 33.800 429 181 13 13 360 12 418 5.01e-55 186
MsG0580030061.01.T01 MTR_5g064700 36.412 379 155 15 56 361 1 366 7.63e-55 184
MsG0580030061.01.T01 MTR_8g013000 34.688 369 170 9 9 323 2 353 9.25e-54 182
MsG0580030061.01.T01 MTR_6g090250 36.029 408 160 19 35 358 29 419 1.19e-53 182
MsG0580030061.01.T01 MTR_2g064950 33.862 378 188 10 9 339 2 364 1.43e-53 182
MsG0580030061.01.T01 MTR_8g046220 34.063 411 192 18 13 362 13 405 4.81e-53 181
MsG0580030061.01.T01 MTR_8g046200 31.973 441 196 15 4 362 3 421 4.84e-53 181
MsG0580030061.01.T01 MTR_7g104710 39.542 306 138 11 1 279 1 286 7.91e-53 177
MsG0580030061.01.T01 MTR_1g050308 34.177 395 154 14 2 361 22 345 5.76e-52 176
MsG0580030061.01.T01 MTR_5g092860 35.328 351 164 14 35 342 20 350 7.85e-52 176
MsG0580030061.01.T01 MTR_8g033110 34.023 435 184 17 6 361 3 413 2.49e-51 176
MsG0580030061.01.T01 MTR_5g097820 39.350 277 110 11 2 234 7 269 8.68e-51 171
MsG0580030061.01.T01 MTR_7g016930 34.637 358 153 10 14 326 13 334 1.22e-50 172
MsG0580030061.01.T01 MTR_1g007430 35.057 348 144 11 28 323 7 324 2.05e-50 171
MsG0580030061.01.T01 MTR_5g023970 49.462 186 85 5 184 361 10 194 3.32e-50 167
MsG0580030061.01.T01 MTR_8g033090 33.255 424 187 16 12 361 9 410 5.09e-50 173
MsG0580030061.01.T01 MTR_6g078010 37.176 347 159 12 54 358 1 330 4.56e-49 169
MsG0580030061.01.T01 MTR_8g018110 31.442 423 193 15 6 361 3 395 9.51e-49 169
MsG0580030061.01.T01 MTR_5g074800 35.854 357 151 12 56 355 1 336 1.06e-48 167
MsG0580030061.01.T01 MTR_8g063330 34.734 357 185 12 3 320 1 348 1.97e-48 170
MsG0580030061.01.T01 MTR_5g097540 38.889 270 128 5 54 323 1 233 2.43e-48 165
MsG0580030061.01.T01 MTR_7g023800 37.231 325 159 14 56 356 1 304 8.23e-48 164
MsG0580030061.01.T01 MTR_1g098180 32.090 402 171 14 11 360 12 363 9.16e-46 160
MsG0580030061.01.T01 MTR_6g090260 48.925 186 82 6 185 358 75 259 2.17e-45 156
MsG0580030061.01.T01 MTR_6g090260 39.474 114 48 2 6 119 9 101 1.73e-17 81.6
MsG0580030061.01.T01 MTR_8g063370 30.488 410 204 17 1 355 1 384 2.36e-44 157
MsG0580030061.01.T01 MTR_4g007940 32.822 326 148 12 93 360 27 339 6.08e-42 150
MsG0580030061.01.T01 MTR_5g043605 32.722 327 155 13 87 360 22 336 3.02e-41 149
MsG0580030061.01.T01 MTR_8g033000 32.105 380 167 14 54 361 1 361 4.58e-41 148
MsG0580030061.01.T01 MTR_6g087620 34.247 292 147 9 94 353 1 279 4.72e-41 145
MsG0580030061.01.T01 MTR_1g050312 36.271 295 126 11 83 342 5 272 4.19e-40 143
MsG0580030061.01.T01 MTR_6g079790 33.750 320 134 10 106 361 2 307 3.95e-38 139
MsG0580030061.01.T01 MTR_5g068490 32.746 284 148 11 103 360 8 274 6.85e-37 134
MsG0580030061.01.T01 MTR_5g022850 28.713 404 165 12 15 361 14 351 1.01e-35 133
MsG0580030061.01.T01 MTR_8g063350 28.889 405 169 18 1 354 1 337 2.64e-35 132
MsG0580030061.01.T01 MTR_5g066420 47.333 150 67 4 214 352 1 149 7.97e-35 125
MsG0580030061.01.T01 MTR_2g021920 43.316 187 92 6 185 359 85 269 2.12e-34 128
MsG0580030061.01.T01 MTR_5g094350 32.353 306 106 9 11 251 7 276 6.89e-34 127
MsG0580030061.01.T01 MTR_6g465360 42.222 180 77 5 7 176 52 214 1.18e-33 126
MsG0580030061.01.T01 MTR_3g018750 39.796 196 93 7 184 359 47 237 1.70e-33 124
MsG0580030061.01.T01 MTR_6g004450 40.323 186 98 6 184 361 87 267 2.64e-33 125
MsG0580030061.01.T01 MTR_8g033140 37.209 215 120 4 2 214 3 204 3.13e-33 128
MsG0580030061.01.T01 MTR_8g033140 36.464 181 107 3 184 361 241 416 1.49e-27 112
MsG0580030061.01.T01 MTR_1g076810 34.323 303 128 13 6 268 8 279 7.65e-33 129
MsG0580030061.01.T01 MTR_5g037190 33.824 272 113 8 6 236 5 250 4.49e-32 121
MsG0580030061.01.T01 MTR_5g076490 35.714 350 167 16 54 361 1 334 1.22e-31 122
MsG0580030061.01.T01 MTR_8g063310 28.788 396 203 21 14 355 19 389 2.20e-31 123
MsG0580030061.01.T01 MTR_6g070880 42.949 156 70 4 14 167 29 167 3.84e-31 119
MsG0580030061.01.T01 MTR_6g071420 42.949 156 70 4 14 167 29 167 3.84e-31 119
MsG0580030061.01.T01 MTR_5g097650 41.379 174 76 4 1 174 6 153 7.69e-31 115
MsG0580030061.01.T01 MTR_7g017150 40.513 195 105 6 176 361 73 265 1.67e-28 112
MsG0580030061.01.T01 MTR_7g017120 40.513 195 105 6 176 361 72 264 1.77e-28 112
MsG0580030061.01.T01 MTR_8g035690 40.884 181 101 4 184 361 124 301 4.60e-28 112
MsG0580030061.01.T01 MTR_5g091150 38.421 190 87 7 106 271 2 185 7.17e-28 108
MsG0580030061.01.T01 MTR_6g079730 46.617 133 66 4 230 358 14 145 2.84e-27 105
MsG0580030061.01.T01 MTR_5g097660 50.962 104 49 1 258 359 13 116 7.28e-27 103
MsG0580030061.01.T01 MTR_8g073570 40.252 159 76 5 9 167 2 141 1.42e-26 104
MsG0580030061.01.T01 MTR_2g104260 34.058 276 112 11 17 238 14 273 4.96e-26 105
MsG0580030061.01.T01 MTR_6g009350 40.523 153 78 6 185 328 47 195 1.95e-25 102
MsG0580030061.01.T01 MTR_1g115205 35.193 233 96 10 17 220 29 235 3.79e-25 102
MsG0580030061.01.T01 MTR_7g019250 39.394 165 80 4 184 343 265 414 1.62e-24 104
MsG0580030061.01.T01 MTR_4g016000 44.776 134 60 5 184 315 69 190 2.47e-24 99.8
MsG0580030061.01.T01 MTR_5g096070 38.272 162 58 4 6 167 15 134 5.48e-24 97.1
MsG0580030061.01.T01 MTR_5g096050 52.830 106 35 4 214 304 1 106 1.66e-23 94.4
MsG0580030061.01.T01 MTR_8g041440 45.802 131 38 6 1 124 1 105 8.66e-22 89.7
MsG0580030061.01.T01 MTR_3g047580 37.580 157 61 5 11 167 8 127 1.15e-21 89.7
MsG0580030061.01.T01 MTR_1g047080 27.042 355 138 14 54 361 1 281 1.37e-21 94.0
MsG0580030061.01.T01 MTR_2g022020 41.584 101 58 1 259 359 17 116 3.07e-21 88.2
MsG0580030061.01.T01 MTR_1g097560 24.479 384 224 13 5 357 24 372 5.42e-21 93.6
MsG0580030061.01.T01 MTR_5g036250 52.439 82 37 2 275 355 23 103 8.21e-20 86.3
MsG0580030061.01.T01 MTR_3g019290 36.458 192 84 10 13 190 10 177 1.94e-19 85.1
MsG0580030061.01.T01 MTR_5g098950 29.717 212 83 7 153 323 88 274 6.57e-19 85.9
MsG0580030061.01.T01 MTR_7g101200 30.476 210 96 8 155 327 40 236 9.26e-18 82.0
MsG0580030061.01.T01 MTR_3g030620 34.756 164 93 6 210 361 19 180 2.10e-17 79.7
MsG0580030061.01.T01 MTR_6g087660 51.948 77 36 1 4 79 5 81 5.19e-17 79.0
MsG0580030061.01.T01 MTR_5g086780 41.748 103 57 2 241 341 127 228 9.05e-17 79.0
MsG0580030061.01.T01 MTR_3g009310 23.824 340 189 12 2 302 21 329 3.25e-15 76.6
MsG0580030061.01.T01 MTR_6g053260 24.658 365 206 16 17 337 337 676 4.43e-15 77.0
MsG0580030061.01.T01 MTR_5g057790 25.545 321 173 14 17 303 46 334 1.18e-14 75.1
MsG0580030061.01.T01 MTR_5g049560 36.066 122 74 2 54 174 1 119 1.50e-14 73.6
MsG0580030061.01.T01 MTR_1g050305 49.398 83 36 3 265 342 21 102 3.80e-14 69.3
MsG0580030061.01.T01 MTR_2g021050 23.677 359 208 15 17 340 5 332 1.40e-13 71.6
MsG0580030061.01.T01 MTR_3g047540 48.438 64 32 1 184 246 5 68 1.41e-13 66.2
MsG0580030061.01.T01 MTR_7g056200 22.959 392 213 18 6 345 12 366 2.31e-13 70.9
MsG0580030061.01.T01 MTR_7g056820 22.901 393 225 16 17 357 7 373 1.78e-12 68.2
MsG0580030061.01.T01 MTR_4g094400 40.367 109 47 3 207 297 3 111 2.29e-12 63.9
MsG0580030061.01.T01 MTR_3g013770 25.000 396 211 17 4 347 20 381 4.43e-12 67.0
MsG0580030061.01.T01 MTR_4g107820 23.034 356 199 14 17 332 36 356 5.20e-12 67.0
MsG0580030061.01.T01 MTR_4g107820 22.500 360 196 13 17 332 36 356 5.48e-12 67.0
MsG0580030061.01.T01 MTR_4g107780 22.500 360 196 13 17 332 36 356 5.48e-12 67.0
MsG0580030061.01.T01 MTR_4g107780 23.034 356 199 14 17 332 36 356 5.80e-12 67.0
MsG0580030061.01.T01 MTR_3g072470 25.134 374 196 17 5 320 7 354 9.53e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580030061.01.T01 AT1G62270 25.641 273 134 11 8 238 2 247 8.94e-13 69.3

Find 88 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGATTTGTATTCTGTTTC+TGG 0.073137 5:+106078643 MsG0580030061.01.T01:CDS
AACTAGCCTTGTTTGATTTA+AGG 0.101987 5:+106079452 MsG0580030061.01.T01:CDS
TTGGTAGAATGCTTTCTTTA+TGG 0.238341 5:-106079528 None:intergenic
TGCTTTCATCTTAGCTTATC+AGG 0.255319 5:+106079276 MsG0580030061.01.T01:CDS
TTAAGTTGTGCTAGTGTTAA+TGG 0.257804 5:+106078766 MsG0580030061.01.T01:CDS
ATTGATAAGATTGGTGTTAC+AGG 0.279465 5:+106079484 MsG0580030061.01.T01:CDS
GTACTGTGATGTTTATAATT+TGG 0.285573 5:+106078818 MsG0580030061.01.T01:CDS
AAAAGATTTAACAGGAAGTT+TGG 0.287725 5:-106078449 None:intergenic
AAATCGAAGATAATTTCTTT+TGG 0.302195 5:+106079008 MsG0580030061.01.T01:CDS
CTCCTCCTTCTACATGTAAA+AGG 0.303122 5:-106078594 None:intergenic
CTAGTCTTTACAAATGGATT+TGG 0.305180 5:-106078700 None:intergenic
TTGGTATTCCTCACCGTTAT+AGG 0.332027 5:+106079074 MsG0580030061.01.T01:CDS
GAAGGAGGAGATGAAAAGTA+TGG 0.332865 5:+106078607 MsG0580030061.01.T01:CDS
AACAGGAAGTTTGGAGAGAA+TGG 0.333785 5:-106078440 None:intergenic
AAGTAGAACACGTACTTATT+TGG 0.338130 5:+106079144 MsG0580030061.01.T01:CDS
AATCGCTTAAAAGATTTAAC+AGG 0.341479 5:-106078457 None:intergenic
GGGCCCTTACCTTATGATCT+TGG 0.363696 5:+106079373 MsG0580030061.01.T01:CDS
AGAAAAGAGTCAAATTAGAT+TGG 0.375321 5:+106078677 MsG0580030061.01.T01:CDS
CATAGATGATGATAGTGTTA+AGG 0.393223 5:+106079219 MsG0580030061.01.T01:CDS
TGGACTAAACTCTTCATTAT+CGG 0.394679 5:+106079352 MsG0580030061.01.T01:CDS
AAAGAAAGCATTCTACCAAT+TGG 0.398241 5:+106079532 MsG0580030061.01.T01:CDS
ATAGATGATGATAGTGTTAA+GGG 0.401882 5:+106079220 MsG0580030061.01.T01:CDS
ACTATCATCATCTATGTAAG+AGG 0.423487 5:-106079212 None:intergenic
GAAACAGAATACAAATCATA+TGG 0.427307 5:-106078640 None:intergenic
ACCTTGAATTCGTCTGTAGT+TGG 0.430152 5:-106078844 None:intergenic
CCTTGAATTCGTCTGTAGTT+GGG 0.433265 5:-106078843 None:intergenic
TCCCCTCTAGTCTTTACAAA+TGG 0.435218 5:-106078706 None:intergenic
AATCATATGTTCCCTCCAAT+TGG 0.448997 5:-106079547 None:intergenic
GCCTTGTTTGATTTAAGGAC+CGG 0.454335 5:+106079457 MsG0580030061.01.T01:CDS
ATCATCTATGTAAGAGGGTA+TGG 0.458203 5:-106079206 None:intergenic
ACACACTTGGTGATATGGAA+TGG 0.473011 5:+106079247 MsG0580030061.01.T01:CDS
TAACTCTTGGAAGAAATTGA+AGG 0.474092 5:+106079051 MsG0580030061.01.T01:CDS
CCGCAACAATTTCTTATCAA+AGG 0.475101 5:+106078540 MsG0580030061.01.T01:CDS
TCTTCCAAGAGTTACTGTTA+AGG 0.476406 5:-106079042 None:intergenic
CACACTTGGTGATATGGAAT+GGG 0.478514 5:+106079248 MsG0580030061.01.T01:CDS
CAGTACCACCGTAGGAAGAA+TGG 0.479700 5:-106078801 None:intergenic
ATAGCCTTAACAGTAACTCT+TGG 0.483209 5:+106079038 MsG0580030061.01.T01:CDS
GGACTAAACTCTTCATTATC+GGG 0.487918 5:+106079353 MsG0580030061.01.T01:CDS
CTTTGTATCCATTCTTCCTA+CGG 0.488080 5:+106078793 MsG0580030061.01.T01:CDS
GATGGAGCGTCTCATTGGTT+AGG 0.488428 5:+106079118 MsG0580030061.01.T01:CDS
CCCAACTACAGACGAATTCA+AGG 0.489253 5:+106078843 MsG0580030061.01.T01:CDS
ATAGGATATCCAAGATCATA+AGG 0.491730 5:-106079382 None:intergenic
ATGGAGAAAACAATATCATC+AGG 0.493354 5:-106078421 None:intergenic
ACCAATCTTATCAATCATTC+CGG 0.497292 5:-106079476 None:intergenic
GAAGAAAGAACAGTACCATA+TGG 0.497723 5:-106078736 None:intergenic
ACCGACTGCAACAACTGTTC+AGG 0.506081 5:+106078381 MsG0580030061.01.T01:CDS
AATAATTGTCATCAAACAAG+AGG 0.506802 5:-106078504 None:intergenic
ACATGGATGGAGCGTCTCAT+TGG 0.508480 5:+106079113 MsG0580030061.01.T01:CDS
ACCGGAATGATTGATAAGAT+TGG 0.510850 5:+106079475 MsG0580030061.01.T01:CDS
CTTGGATATCCTATCGGAGG+GGG 0.523849 5:+106079391 MsG0580030061.01.T01:CDS
CTTGTTCATCAATCCTATAA+CGG 0.530922 5:-106079087 None:intergenic
ATATCCAAGATCATAAGGTA+AGG 0.536973 5:-106079377 None:intergenic
ATCTTGGATATCCTATCGGA+GGG 0.538466 5:+106079389 MsG0580030061.01.T01:CDS
ATCCATTTGTAAAGACTAGA+GGG 0.540392 5:+106078704 MsG0580030061.01.T01:CDS
AGAGGGGACCCATCACCATA+TGG 0.542277 5:+106078721 MsG0580030061.01.T01:CDS
AGAACAGTACCATATGGTGA+TGG 0.547424 5:-106078730 None:intergenic
TTCATCTTAGCTTATCAGGA+GGG 0.552483 5:+106079280 MsG0580030061.01.T01:CDS
GAAAGCATTCTACCAATTGG+AGG 0.565886 5:+106079535 MsG0580030061.01.T01:CDS
TCTTGGAAGAAATTGAAGGT+TGG 0.570503 5:+106079055 MsG0580030061.01.T01:CDS
TATGATCTTGGATATCCTAT+CGG 0.573636 5:+106079385 MsG0580030061.01.T01:CDS
AATCCATTTGTAAAGACTAG+AGG 0.576405 5:+106078703 MsG0580030061.01.T01:CDS
ACAGAATACAAATCATATGG+AGG 0.588650 5:-106078637 None:intergenic
CGTAGGAAGAATGGATACAA+AGG 0.590097 5:-106078792 None:intergenic
GATTGATGAACAAGTGTACA+TGG 0.595954 5:+106079096 MsG0580030061.01.T01:CDS
GAACAGTACCATATGGTGAT+GGG 0.596455 5:-106078729 None:intergenic
TTGGATATCCTATCGGAGGG+GGG 0.601228 5:+106079392 MsG0580030061.01.T01:CDS
GCAGTCGGTGATATCGCCAT+AGG 0.601535 5:-106078367 None:intergenic
GTAGGAAGAATGGATACAAA+GGG 0.603151 5:-106078791 None:intergenic
ACCTGAACAGTTGTTGCAGT+CGG 0.604635 5:-106078382 None:intergenic
AACTTGGAATAAAAGAATCG+TGG 0.605926 5:+106079332 MsG0580030061.01.T01:CDS
AAAGCATTCTACCAATTGGA+GGG 0.614043 5:+106079536 MsG0580030061.01.T01:CDS
CAGAATACAAATCATATGGA+GGG 0.619207 5:-106078636 None:intergenic
TTGAATGTGTGAGTAAATCA+TGG 0.619771 5:+106078479 MsG0580030061.01.T01:CDS
GATCTTGGATATCCTATCGG+AGG 0.621126 5:+106079388 MsG0580030061.01.T01:CDS
CTATCATCATCTATGTAAGA+GGG 0.621318 5:-106079211 None:intergenic
TATCCAAGATCATAAGGTAA+GGG 0.621592 5:-106079376 None:intergenic
ATAATTGTCATCAAACAAGA+GGG 0.622232 5:-106078503 None:intergenic
TGGATATCCTATCGGAGGGG+GGG 0.626789 5:+106079393 MsG0580030061.01.T01:CDS
TGTATCCATTCTTCCTACGG+TGG 0.628368 5:+106078796 MsG0580030061.01.T01:CDS
TCCGGTCCTTAAATCAAACA+AGG 0.638060 5:-106079458 None:intergenic
TTTCATCTTAGCTTATCAGG+AGG 0.644336 5:+106079279 MsG0580030061.01.T01:CDS
GATGAACAAGTGTACATGGA+TGG 0.652316 5:+106079100 MsG0580030061.01.T01:CDS
TCGTCGTCATGTGAGTCATG+TGG 0.665209 5:-106078986 None:intergenic
CCTTTGATAAGAAATTGTTG+CGG 0.668964 5:-106078540 None:intergenic
AAACATCACAGTACCACCGT+AGG 0.683759 5:-106078809 None:intergenic
TCTTGGATATCCTATCGGAG+GGG 0.700683 5:+106079390 MsG0580030061.01.T01:CDS
TCCATTTGTAAAGACTAGAG+GGG 0.701697 5:+106078705 MsG0580030061.01.T01:CDS
TCATCAATCCTATAACGGTG+AGG 0.711530 5:-106079082 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATCGAAGATAATTTCTTT+TGG + Chr5:106079008-106079027 MsG0580030061.01.T01:CDS 20.0%
!!! AATCGAAGATAATTTCTTTT+GGG + Chr5:106079009-106079028 MsG0580030061.01.T01:CDS 20.0%
!!! ATATTTCACATTTCAGTTTT+AGG + Chr5:106079307-106079326 MsG0580030061.01.T01:CDS 20.0%
!!! TATTTCACATTTCAGTTTTA+GGG + Chr5:106079308-106079327 MsG0580030061.01.T01:CDS 20.0%
!!! TTGAATTTCCTGAATTTTAT+TGG + Chr5:106078881-106078900 MsG0580030061.01.T01:intron 20.0%
! AAAAGATTTAACAGGAAGTT+TGG - Chr5:106078452-106078471 None:intergenic 25.0%
! AATAATTGTCATCAAACAAG+AGG - Chr5:106078507-106078526 None:intergenic 25.0%
! AATCGCTTAAAAGATTTAAC+AGG - Chr5:106078460-106078479 None:intergenic 25.0%
! AGAAAAGAGTCAAATTAGAT+TGG + Chr5:106078677-106078696 MsG0580030061.01.T01:CDS 25.0%
! ATAATTGTCATCAAACAAGA+GGG - Chr5:106078506-106078525 None:intergenic 25.0%
! GAAACAGAATACAAATCATA+TGG - Chr5:106078643-106078662 None:intergenic 25.0%
! GTACTGTGATGTTTATAATT+TGG + Chr5:106078818-106078837 MsG0580030061.01.T01:CDS 25.0%
!! AATTTCCTGAATTTTATTGG+CGG + Chr5:106078884-106078903 MsG0580030061.01.T01:intron 25.0%
!! ATAGATGATGATAGTGTTAA+GGG + Chr5:106079220-106079239 MsG0580030061.01.T01:CDS 25.0%
!! CTATATAGTCCTTTTTAACA+CGG - Chr5:106078942-106078961 None:intergenic 25.0%
!! TATGATTTGTATTCTGTTTC+TGG + Chr5:106078643-106078662 MsG0580030061.01.T01:CDS 25.0%
!!! ATTTCACATTTCAGTTTTAG+GGG + Chr5:106079309-106079328 MsG0580030061.01.T01:CDS 25.0%
AAATTCAGGAAATTCAAAGC+TGG - Chr5:106078878-106078897 None:intergenic 30.0%
AACTTGGAATAAAAGAATCG+TGG + Chr5:106079332-106079351 MsG0580030061.01.T01:CDS 30.0%
AAGTAGAACACGTACTTATT+TGG + Chr5:106079144-106079163 MsG0580030061.01.T01:CDS 30.0%
AATCCATTTGTAAAGACTAG+AGG + Chr5:106078703-106078722 MsG0580030061.01.T01:CDS 30.0%
ACAGAATACAAATCATATGG+AGG - Chr5:106078640-106078659 None:intergenic 30.0%
ACCAATCTTATCAATCATTC+CGG - Chr5:106079479-106079498 None:intergenic 30.0%
ACTATCATCATCTATGTAAG+AGG - Chr5:106079215-106079234 None:intergenic 30.0%
ATAGGATATCCAAGATCATA+AGG - Chr5:106079385-106079404 None:intergenic 30.0%
ATATCCAAGATCATAAGGTA+AGG - Chr5:106079380-106079399 None:intergenic 30.0%
ATCCATTTGTAAAGACTAGA+GGG + Chr5:106078704-106078723 MsG0580030061.01.T01:CDS 30.0%
ATGGAGAAAACAATATCATC+AGG - Chr5:106078424-106078443 None:intergenic 30.0%
CAGAATACAAATCATATGGA+GGG - Chr5:106078639-106078658 None:intergenic 30.0%
CCTTTGATAAGAAATTGTTG+CGG - Chr5:106078543-106078562 None:intergenic 30.0%
CTATCATCATCTATGTAAGA+GGG - Chr5:106079214-106079233 None:intergenic 30.0%
CTTGTTCATCAATCCTATAA+CGG - Chr5:106079090-106079109 None:intergenic 30.0%
GTCATAACCAAATAGATGAT+TGG - Chr5:106078920-106078939 None:intergenic 30.0%
TAACTCTTGGAAGAAATTGA+AGG + Chr5:106079051-106079070 MsG0580030061.01.T01:CDS 30.0%
TACACAACCAATCATCTATT+TGG + Chr5:106078910-106078929 MsG0580030061.01.T01:intron 30.0%
TATCCAAGATCATAAGGTAA+GGG - Chr5:106079379-106079398 None:intergenic 30.0%
TATGATCTTGGATATCCTAT+CGG + Chr5:106079385-106079404 MsG0580030061.01.T01:CDS 30.0%
TGGACTAAACTCTTCATTAT+CGG + Chr5:106079352-106079371 MsG0580030061.01.T01:CDS 30.0%
TTGAATGTGTGAGTAAATCA+TGG + Chr5:106078479-106078498 MsG0580030061.01.T01:CDS 30.0%
TTGGTAGAATGCTTTCTTTA+TGG - Chr5:106079531-106079550 None:intergenic 30.0%
! AATAGATGATTGGTTGTGTA+AGG - Chr5:106078910-106078929 None:intergenic 30.0%
! CATAGATGATGATAGTGTTA+AGG + Chr5:106079219-106079238 MsG0580030061.01.T01:CDS 30.0%
! CTAGTCTTTACAAATGGATT+TGG - Chr5:106078703-106078722 None:intergenic 30.0%
! TTAAGTTGTGCTAGTGTTAA+TGG + Chr5:106078766-106078785 MsG0580030061.01.T01:CDS 30.0%
!! AAAGAAAGCATTCTACCAAT+TGG + Chr5:106079532-106079551 MsG0580030061.01.T01:CDS 30.0%
!! AACTAGCCTTGTTTGATTTA+AGG + Chr5:106079452-106079471 MsG0580030061.01.T01:CDS 30.0%
!! ATTGATAAGATTGGTGTTAC+AGG + Chr5:106079484-106079503 MsG0580030061.01.T01:CDS 30.0%
ACCGGAATGATTGATAAGAT+TGG + Chr5:106079475-106079494 MsG0580030061.01.T01:CDS 35.0%
ATCATCTATGTAAGAGGGTA+TGG - Chr5:106079209-106079228 None:intergenic 35.0%
CCGCAACAATTTCTTATCAA+AGG + Chr5:106078540-106078559 MsG0580030061.01.T01:CDS 35.0%
CTTTGTATCCATTCTTCCTA+CGG + Chr5:106078793-106078812 MsG0580030061.01.T01:CDS 35.0%
GAAGAAAGAACAGTACCATA+TGG - Chr5:106078739-106078758 None:intergenic 35.0%
GATTGATGAACAAGTGTACA+TGG + Chr5:106079096-106079115 MsG0580030061.01.T01:CDS 35.0%
GGACTAAACTCTTCATTATC+GGG + Chr5:106079353-106079372 MsG0580030061.01.T01:CDS 35.0%
GTAGGAAGAATGGATACAAA+GGG - Chr5:106078794-106078813 None:intergenic 35.0%
TCCATTTGTAAAGACTAGAG+GGG + Chr5:106078705-106078724 MsG0580030061.01.T01:CDS 35.0%
TCTTCCAAGAGTTACTGTTA+AGG - Chr5:106079045-106079064 None:intergenic 35.0%
TCTTGGAAGAAATTGAAGGT+TGG + Chr5:106079055-106079074 MsG0580030061.01.T01:CDS 35.0%
TGCTTTCATCTTAGCTTATC+AGG + Chr5:106079276-106079295 MsG0580030061.01.T01:CDS 35.0%
TGGTTATGACCGTGTTAAAA+AGG + Chr5:106078930-106078949 MsG0580030061.01.T01:intron 35.0%
TGTTAAGGGAAAAACACACT+TGG + Chr5:106079234-106079253 MsG0580030061.01.T01:CDS 35.0%
TTCATCTTAGCTTATCAGGA+GGG + Chr5:106079280-106079299 MsG0580030061.01.T01:CDS 35.0%
TTTCATCTTAGCTTATCAGG+AGG + Chr5:106079279-106079298 MsG0580030061.01.T01:CDS 35.0%
! AAAGCATTCTACCAATTGGA+GGG + Chr5:106079536-106079555 MsG0580030061.01.T01:CDS 35.0%
! ATAGCCTTAACAGTAACTCT+TGG + Chr5:106079038-106079057 MsG0580030061.01.T01:CDS 35.0%
! GAAAAACACACTTGGTGATA+TGG + Chr5:106079242-106079261 MsG0580030061.01.T01:CDS 35.0%
! TCTCTCCTTTTACATGTAGA+AGG + Chr5:106078589-106078608 MsG0580030061.01.T01:CDS 35.0%
!!! ATTTCAGTTTTAGGGGAACT+TGG + Chr5:106079316-106079335 MsG0580030061.01.T01:CDS 35.0%
AACAGGAAGTTTGGAGAGAA+TGG - Chr5:106078443-106078462 None:intergenic 40.0%
ACCTTGAATTCGTCTGTAGT+TGG - Chr5:106078847-106078866 None:intergenic 40.0%
AGAACAGTACCATATGGTGA+TGG - Chr5:106078733-106078752 None:intergenic 40.0%
ATCTTGGATATCCTATCGGA+GGG + Chr5:106079389-106079408 MsG0580030061.01.T01:CDS 40.0%
CCTTGAATTCGTCTGTAGTT+GGG - Chr5:106078846-106078865 None:intergenic 40.0%
CGTAGGAAGAATGGATACAA+AGG - Chr5:106078795-106078814 None:intergenic 40.0%
CTCCTCCTTCTACATGTAAA+AGG - Chr5:106078597-106078616 None:intergenic 40.0%
GAACAGTACCATATGGTGAT+GGG - Chr5:106078732-106078751 None:intergenic 40.0%
GAAGGAGGAGATGAAAAGTA+TGG + Chr5:106078607-106078626 MsG0580030061.01.T01:CDS 40.0%
GATGAACAAGTGTACATGGA+TGG + Chr5:106079100-106079119 MsG0580030061.01.T01:CDS 40.0%
TAAGGCCGCCAATAAAATTC+AGG - Chr5:106078892-106078911 None:intergenic 40.0%
TCAGGAAATTCAAAGCTGGA+AGG - Chr5:106078874-106078893 None:intergenic 40.0%
TCATCAATCCTATAACGGTG+AGG - Chr5:106079085-106079104 None:intergenic 40.0%
TCCGGTCCTTAAATCAAACA+AGG - Chr5:106079461-106079480 None:intergenic 40.0%
TTGGTATTCCTCACCGTTAT+AGG + Chr5:106079074-106079093 MsG0580030061.01.T01:CDS 40.0%
! ACACACTTGGTGATATGGAA+TGG + Chr5:106079247-106079266 MsG0580030061.01.T01:CDS 40.0%
! CACACTTGGTGATATGGAAT+GGG + Chr5:106079248-106079267 MsG0580030061.01.T01:CDS 40.0%
! CTCCTTTTACATGTAGAAGG+AGG + Chr5:106078592-106078611 MsG0580030061.01.T01:CDS 40.0%
! TCCCCTCTAGTCTTTACAAA+TGG - Chr5:106078709-106078728 None:intergenic 40.0%
!! GAAAGCATTCTACCAATTGG+AGG + Chr5:106079535-106079554 MsG0580030061.01.T01:CDS 40.0%
!! GCCTTGTTTGATTTAAGGAC+CGG + Chr5:106079457-106079476 MsG0580030061.01.T01:CDS 40.0%
AAACATCACAGTACCACCGT+AGG - Chr5:106078812-106078831 None:intergenic 45.0%
ACCTGAACAGTTGTTGCAGT+CGG - Chr5:106078385-106078404 None:intergenic 45.0%
CCCAACTACAGACGAATTCA+AGG + Chr5:106078843-106078862 MsG0580030061.01.T01:CDS 45.0%
GATCTTGGATATCCTATCGG+AGG + Chr5:106079388-106079407 MsG0580030061.01.T01:CDS 45.0%
TCTTGGATATCCTATCGGAG+GGG + Chr5:106079390-106079409 MsG0580030061.01.T01:CDS 45.0%
TGTATCCATTCTTCCTACGG+TGG + Chr5:106078796-106078815 MsG0580030061.01.T01:CDS 45.0%
ACCGACTGCAACAACTGTTC+AGG + Chr5:106078381-106078400 MsG0580030061.01.T01:CDS 50.0%
CAGTACCACCGTAGGAAGAA+TGG - Chr5:106078804-106078823 None:intergenic 50.0%
CTTGGATATCCTATCGGAGG+GGG + Chr5:106079391-106079410 MsG0580030061.01.T01:CDS 50.0%
GGGCCCTTACCTTATGATCT+TGG + Chr5:106079373-106079392 MsG0580030061.01.T01:CDS 50.0%
TCGTCGTCATGTGAGTCATG+TGG - Chr5:106078989-106079008 None:intergenic 50.0%
TTGGATATCCTATCGGAGGG+GGG + Chr5:106079392-106079411 MsG0580030061.01.T01:CDS 50.0%
! ACATGGATGGAGCGTCTCAT+TGG + Chr5:106079113-106079132 MsG0580030061.01.T01:CDS 50.0%
! GATGGAGCGTCTCATTGGTT+AGG + Chr5:106079118-106079137 MsG0580030061.01.T01:CDS 50.0%
AGAGGGGACCCATCACCATA+TGG + Chr5:106078721-106078740 MsG0580030061.01.T01:CDS 55.0%
TGGATATCCTATCGGAGGGG+GGG + Chr5:106079393-106079412 MsG0580030061.01.T01:CDS 55.0%
CCTATCGGAGGGGGGGAGAA+AGG + Chr5:106079400-106079419 MsG0580030061.01.T01:CDS 65.0%
CCTTTCTCCCCCCCTCCGAT+AGG - Chr5:106079403-106079422 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 106078370 106079567 106078370 ID=MsG0580030061.01;Name=MsG0580030061.01
Chr5 mRNA 106078370 106079567 106078370 ID=MsG0580030061.01.T01;Parent=MsG0580030061.01;Name=MsG0580030061.01.T01;_AED=0.28;_eAED=0.28;_QI=0|0|0|1|1|1|2|0|363
Chr5 exon 106078370 106078864 106078370 ID=MsG0580030061.01.T01:exon:14501;Parent=MsG0580030061.01.T01
Chr5 exon 106078971 106079567 106078971 ID=MsG0580030061.01.T01:exon:14502;Parent=MsG0580030061.01.T01
Chr5 CDS 106078370 106078864 106078370 ID=MsG0580030061.01.T01:cds;Parent=MsG0580030061.01.T01
Chr5 CDS 106078971 106079567 106078971 ID=MsG0580030061.01.T01:cds;Parent=MsG0580030061.01.T01
Gene Sequence

>MsG0580030061.01.T01

ATGGCGATATCACCGACTGCAACAACTGTTCAGGTTAGCAGCAGTTATGTGCCTGATGATATTGTTTTCTCCATTCTCTCCAAACTTCCTGTTAAATCTTTTAAGCGATTTGAATGTGTGAGTAAATCATGGTCCCTCTTGTTTGATGACAATTATTTTATGAATATGTACCGCAACAATTTCTTATCAAAGGTTTCTTCTTTTTATCATGATCATACATCTCTCCTTTTACATGTAGAAGGAGGAGATGAAAAGTATGGAGAATACCCTCCATATGATTTGTATTCTGTTTCTGGAGAGAGATTTGAGAAAAGAGTCAAATTAGATTGGCCAAATCCATTTGTAAAGACTAGAGGGGACCCATCACCATATGGTACTGTTCTTTCTTCTAAATTGTTAAGTTGTGCTAGTGTTAATGGCACCCTTTGTATCCATTCTTCCTACGGTGGTACTGTGATGTTTATAATTTGGAACCCAACTACAGACGAATTCAAGTTCGTACATGAGATACCACATGACTCACATGACGACGAAATCGAAGATAATTTCTTTTGGGAGATTTATAGCCTTAACAGTAACTCTTGGAAGAAATTGAAGGTTGGTATTCCTCACCGTTATAGGATTGATGAACAAGTGTACATGGATGGAGCGTCTCATTGGTTAGGTGAAAGTAGAACACGTACTTATTTGGTGTCGTTTGACTTCAGCAGTGAATCTTGCATTATAACATCCATACCCTCTTACATAGATGATGATAGTGTTAAGGGAAAAACACACTTGGTGATATGGAATGGGTATATTGCTTTCATCTTAGCTTATCAGGAGGGATCTATATTTCACATTTCAGTTTTAGGGGAACTTGGAATAAAAGAATCGTGGACTAAACTCTTCATTATCGGGCCCTTACCTTATGATCTTGGATATCCTATCGGAGGGGGGGAGAAAGGCAAGATGTTGTTTAGAAGAAAAAATGATAAACTAGCCTTGTTTGATTTAAGGACCGGAATGATTGATAAGATTGGTGTTACAGGAGAGAGATGTGACATACTATTCCATAAAGAAAGCATTCTACCAATTGGAGGGAACATATGA
Protein sequence

>MsG0580030061.01.T01

MAISPTATTVQVSSSYVPDDIVFSILSKLPVKSFKRFECVSKSWSLLFDDNYFMNMYRNNFLSKVSSFYHDHTSLLLHVEGGDEKYGEYPPYDLYSVSGERFEKRVKLDWPNPFVKTRGDPSPYGTVLSSKLLSCASVNGTLCIHSSYGGTVMFIIWNPTTDEFKFVHEIPHDSHDDEIEDNFFWEIYSLNSNSWKKLKVGIPHRYRIDEQVYMDGASHWLGESRTRTYLVSFDFSSESCIITSIPSYIDDDSVKGKTHLVIWNGYIAFILAYQEGSIFHISVLGELGIKESWTKLFIIGPLPYDLGYPIGGGEKGKMLFRRKNDKLALFDLRTGMIDKIGVTGERCDILFHKESILPIGGNI*