AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680034881.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034881.01.T01 MTR_6g080680 70.870 230 62 4 8 235 1 227 2.86e-103 306
MsG0680034881.01.T01 MTR_0134s0060 53.419 234 84 4 25 235 10 241 2.25e-71 226
MsG0680034881.01.T01 MTR_6g070880 47.104 259 112 6 2 241 10 262 6.46e-70 217
MsG0680034881.01.T01 MTR_6g071420 47.104 259 112 6 2 241 10 262 6.46e-70 217
MsG0680034881.01.T01 MTR_4g028660 52.863 227 95 4 15 233 4 226 6.35e-69 219
MsG0680034881.01.T01 MTR_4g027450 52.863 227 95 4 15 233 4 226 6.35e-69 219
MsG0680034881.01.T01 MTR_6g465360 49.593 246 96 6 3 225 41 281 6.38e-67 210
MsG0680034881.01.T01 MTR_4g028680 52.790 233 87 5 15 233 4 227 7.19e-67 214
MsG0680034881.01.T01 MTR_4g028510 51.754 228 99 4 15 233 4 229 2.63e-65 211
MsG0680034881.01.T01 MTR_6g079770 51.394 251 94 6 8 235 1 246 3.46e-65 210
MsG0680034881.01.T01 MTR_6g079810 48.699 269 105 8 7 253 53 310 1.68e-64 209
MsG0680034881.01.T01 MTR_0054s0090 51.012 247 93 8 8 235 1 238 1.78e-64 207
MsG0680034881.01.T01 MTR_4g028750 52.193 228 92 7 15 231 4 225 8.67e-63 203
MsG0680034881.01.T01 MTR_0054s0100 50.000 248 97 8 7 235 1 240 1.11e-62 203
MsG0680034881.01.T01 MTR_7g104710 50.000 228 81 3 8 235 1 195 2.88e-62 199
MsG0680034881.01.T01 MTR_6g086825 52.966 236 87 7 15 235 58 284 5.59e-62 202
MsG0680034881.01.T01 MTR_4g130330 50.446 224 96 5 21 235 1 218 1.98e-58 191
MsG0680034881.01.T01 MTR_6g090180 47.303 241 99 8 16 235 37 270 8.56e-54 181
MsG0680034881.01.T01 MTR_6g090250 47.489 219 83 5 45 233 29 245 1.89e-52 177
MsG0680034881.01.T01 MTR_2g034700 46.696 227 102 4 20 239 7 221 1.94e-49 168
MsG0680034881.01.T01 MTR_4g117550 44.400 250 98 11 8 235 1 231 8.13e-49 166
MsG0680034881.01.T01 MTR_8g039500 44.737 228 99 8 16 236 2 209 1.24e-48 165
MsG0680034881.01.T01 MTR_2g022000 40.977 266 102 8 8 238 1 246 2.55e-48 166
MsG0680034881.01.T01 MTR_8g012090 43.750 240 92 7 8 240 1 204 4.21e-48 163
MsG0680034881.01.T01 MTR_0129s0070 42.339 248 105 7 7 235 1 229 4.66e-48 165
MsG0680034881.01.T01 MTR_4g055820 42.460 252 104 8 14 240 1 236 3.70e-47 161
MsG0680034881.01.T01 MTR_7g017170 44.262 244 105 9 8 235 1 229 1.35e-46 161
MsG0680034881.01.T01 MTR_7g017130 44.262 244 105 9 8 235 1 229 1.40e-46 161
MsG0680034881.01.T01 MTR_2g065860 45.217 230 108 8 16 236 2 222 1.82e-46 160
MsG0680034881.01.T01 MTR_2g021800 40.075 267 104 9 8 238 1 247 2.28e-46 160
MsG0680034881.01.T01 MTR_1g076810 41.909 241 109 7 8 234 1 224 2.90e-46 165
MsG0680034881.01.T01 MTR_4g102410 42.688 253 109 10 5 236 41 278 1.08e-45 160
MsG0680034881.01.T01 MTR_3g011120 43.830 235 103 10 14 235 1 219 1.34e-45 157
MsG0680034881.01.T01 MTR_8g064890 44.355 248 110 8 15 246 4 239 1.76e-45 158
MsG0680034881.01.T01 MTR_5g024260 45.726 234 99 9 15 235 10 228 5.10e-45 157
MsG0680034881.01.T01 MTR_5g059430 38.188 309 123 13 8 267 1 290 1.16e-44 156
MsG0680034881.01.T01 MTR_5g037680 44.156 231 94 8 23 236 4 216 1.23e-44 157
MsG0680034881.01.T01 MTR_6g478230 44.583 240 103 8 8 235 1 222 8.35e-44 154
MsG0680034881.01.T01 MTR_0129s0120 40.726 248 109 7 7 235 1 229 9.05e-44 153
MsG0680034881.01.T01 MTR_1g050308 41.870 246 105 11 6 236 15 237 2.57e-43 151
MsG0680034881.01.T01 MTR_0129s0050 42.636 258 89 11 16 235 4 240 3.57e-43 152
MsG0680034881.01.T01 MTR_5g096630 41.833 251 108 10 8 236 1 235 1.70e-42 150
MsG0680034881.01.T01 MTR_7g017040 43.750 240 102 9 14 237 4 226 2.05e-42 150
MsG0680034881.01.T01 MTR_5g069390 40.230 261 108 8 8 235 1 246 4.07e-42 151
MsG0680034881.01.T01 MTR_5g096900 42.500 240 99 8 17 235 2 223 4.19e-42 147
MsG0680034881.01.T01 MTR_8g467670 43.668 229 98 9 22 235 9 221 4.91e-42 149
MsG0680034881.01.T01 MTR_2g021950 40.650 246 114 9 8 238 1 229 7.19e-42 148
MsG0680034881.01.T01 MTR_5g074850 40.083 242 114 6 14 235 22 252 1.43e-41 153
MsG0680034881.01.T01 MTR_5g097220 38.340 253 117 7 8 235 1 239 2.33e-41 147
MsG0680034881.01.T01 MTR_8g046310 41.558 231 104 5 13 232 2 212 5.09e-41 145
MsG0680034881.01.T01 MTR_6g078010 46.243 173 83 3 64 235 1 164 9.23e-41 144
MsG0680034881.01.T01 MTR_7g079640 42.857 217 94 9 26 235 12 205 1.29e-40 145
MsG0680034881.01.T01 MTR_1g053510 38.735 253 107 8 15 232 3 242 1.75e-40 145
MsG0680034881.01.T01 MTR_1g007410 38.403 263 111 9 4 237 3 243 2.76e-40 144
MsG0680034881.01.T01 MTR_8g033140 37.591 274 112 10 11 238 2 262 4.50e-40 144
MsG0680034881.01.T01 MTR_2g066200 40.948 232 111 9 16 236 2 218 7.35e-40 143
MsG0680034881.01.T01 MTR_6g461660 41.200 250 109 8 7 233 1 235 1.23e-39 142
MsG0680034881.01.T01 MTR_2g065840 37.600 250 128 9 16 255 2 233 1.29e-39 142
MsG0680034881.01.T01 MTR_0831s0010 37.860 243 104 8 15 235 38 255 1.41e-39 143
MsG0680034881.01.T01 MTR_5g097680 40.816 245 106 9 24 233 14 254 1.47e-39 143
MsG0680034881.01.T01 MTR_5g097820 38.934 244 110 8 15 236 10 236 3.46e-39 138
MsG0680034881.01.T01 MTR_2g066170 41.126 231 110 8 16 235 2 217 3.77e-39 141
MsG0680034881.01.T01 MTR_2g038680 40.392 255 107 12 14 235 4 246 1.07e-38 140
MsG0680034881.01.T01 MTR_5g097350 41.600 250 102 11 8 235 1 228 1.35e-38 139
MsG0680034881.01.T01 MTR_8g063440 40.084 237 109 7 14 235 1 219 1.55e-38 139
MsG0680034881.01.T01 MTR_7g069550 37.903 248 113 7 14 235 1 233 1.80e-38 139
MsG0680034881.01.T01 MTR_5g097330 38.645 251 106 10 14 235 5 236 2.26e-38 139
MsG0680034881.01.T01 MTR_4g014370 37.743 257 117 10 24 251 8 250 6.02e-38 138
MsG0680034881.01.T01 MTR_5g092850 39.095 243 108 10 16 235 5 230 6.34e-38 138
MsG0680034881.01.T01 MTR_8g013000 39.831 236 103 7 25 235 8 229 1.22e-37 137
MsG0680034881.01.T01 MTR_7g016930 41.453 234 90 9 19 237 9 210 2.09e-37 135
MsG0680034881.01.T01 MTR_2g021770 38.683 243 97 10 27 235 12 236 3.23e-37 136
MsG0680034881.01.T01 MTR_2g104340 36.992 246 109 8 14 235 1 224 4.10e-37 136
MsG0680034881.01.T01 MTR_1g007430 38.333 240 106 10 38 253 7 228 1.29e-36 133
MsG0680034881.01.T01 MTR_1g007450 37.500 256 119 10 16 248 2 239 1.79e-36 135
MsG0680034881.01.T01 MTR_5g072060 40.359 223 95 7 25 224 4 211 3.02e-36 136
MsG0680034881.01.T01 MTR_5g094350 38.462 234 103 6 24 237 10 222 3.85e-36 130
MsG0680034881.01.T01 MTR_5g075370 40.586 239 107 9 21 235 1 228 6.66e-36 136
MsG0680034881.01.T01 MTR_2g066150 43.284 201 84 8 26 218 11 189 1.47e-35 131
MsG0680034881.01.T01 MTR_8g464770 39.732 224 109 6 24 235 9 218 2.01e-35 131
MsG0680034881.01.T01 MTR_5g096910 38.723 235 109 9 21 235 11 230 5.51e-35 130
MsG0680034881.01.T01 MTR_5g091140 36.187 257 100 8 3 235 26 242 7.80e-35 130
MsG0680034881.01.T01 MTR_8g033090 35.115 262 120 9 14 239 1 248 8.48e-35 130
MsG0680034881.01.T01 MTR_8g064790 44.792 192 86 7 15 202 4 179 2.18e-34 127
MsG0680034881.01.T01 MTR_5g037190 41.277 235 101 8 8 222 1 218 3.45e-34 125
MsG0680034881.01.T01 MTR_8g073570 42.391 184 78 6 25 201 8 170 6.05e-34 122
MsG0680034881.01.T01 MTR_8g033110 35.433 254 115 8 14 232 1 240 7.31e-34 127
MsG0680034881.01.T01 MTR_8g046200 36.042 283 86 9 15 232 4 256 2.44e-33 126
MsG0680034881.01.T01 MTR_2g104280 36.434 258 103 11 14 235 1 233 2.62e-33 126
MsG0680034881.01.T01 MTR_7g023800 51.079 139 64 3 66 202 1 137 3.75e-32 120
MsG0680034881.01.T01 MTR_8g041270 35.547 256 114 10 15 239 3 238 5.15e-32 122
MsG0680034881.01.T01 MTR_8g063330 37.500 240 113 9 16 234 2 225 7.08e-31 120
MsG0680034881.01.T01 MTR_8g018110 33.858 254 118 7 14 231 1 240 1.59e-30 118
MsG0680034881.01.T01 MTR_8g046220 34.241 257 127 7 13 249 3 237 1.91e-30 118
MsG0680034881.01.T01 MTR_1g098180 37.281 228 98 9 8 224 1 194 4.39e-29 114
MsG0680034881.01.T01 MTR_5g097650 44.099 161 75 6 8 165 1 149 6.16e-29 108
MsG0680034881.01.T01 MTR_1g115205 35.878 262 107 11 1 246 10 226 8.12e-29 110
MsG0680034881.01.T01 MTR_5g096070 41.361 191 60 7 14 197 13 158 1.52e-28 107
MsG0680034881.01.T01 MTR_7g111700 36.965 257 97 11 8 232 1 224 3.89e-28 111
MsG0680034881.01.T01 MTR_8g032980 34.981 263 106 10 13 232 2 242 5.02e-28 112
MsG0680034881.01.T01 MTR_5g043605 32.636 239 93 5 8 241 1 176 8.69e-28 110
MsG0680034881.01.T01 MTR_2g104260 38.039 255 103 10 14 235 1 233 1.86e-27 107
MsG0680034881.01.T01 MTR_6g087660 53.097 113 49 2 8 118 1 111 1.09e-26 103
MsG0680034881.01.T01 MTR_2g064950 34.177 237 108 7 25 237 8 220 2.19e-26 107
MsG0680034881.01.T01 MTR_8g036970 36.744 215 78 7 31 237 2 166 2.42e-25 103
MsG0680034881.01.T01 MTR_8g063370 35.023 217 121 6 23 232 20 223 2.62e-25 104
MsG0680034881.01.T01 MTR_8g063310 33.191 235 129 8 11 237 6 220 2.99e-25 103
MsG0680034881.01.T01 MTR_5g092860 33.019 212 97 7 45 235 20 207 4.81e-25 103
MsG0680034881.01.T01 MTR_3g019290 33.333 228 82 7 16 224 2 178 2.62e-23 94.4
MsG0680034881.01.T01 MTR_4g014370 41.259 143 69 5 4 138 2 137 1.72e-21 92.8
MsG0680034881.01.T01 MTR_3g047580 40.972 144 63 5 14 157 1 122 4.98e-20 84.0
MsG0680034881.01.T01 MTR_8g063350 31.390 223 101 8 14 233 1 174 6.54e-20 88.6
MsG0680034881.01.T01 MTR_6g079790 43.796 137 59 5 110 235 2 131 1.64e-19 87.0
MsG0680034881.01.T01 MTR_5g022850 32.468 231 77 6 25 234 14 186 2.15e-19 87.0
MsG0680034881.01.T01 MTR_5g074800 34.314 204 80 8 66 238 1 181 2.45e-19 86.7
MsG0680034881.01.T01 MTR_6g090260 59.420 69 28 0 8 76 1 69 6.16e-19 84.3
MsG0680034881.01.T01 MTR_1g050312 36.054 147 66 5 98 236 16 142 8.86e-19 84.3
MsG0680034881.01.T01 MTR_4g007940 32.044 181 90 5 77 237 2 169 9.33e-18 82.4
MsG0680034881.01.T01 MTR_2g046900 28.745 247 126 10 1 231 1 213 1.28e-16 79.3
MsG0680034881.01.T01 MTR_1g097330 33.333 213 100 12 27 231 16 194 2.78e-16 78.6
MsG0680034881.01.T01 MTR_8g041440 48.315 89 29 2 26 114 14 85 4.56e-16 73.2
MsG0680034881.01.T01 MTR_5g064700 33.000 200 103 8 66 237 1 197 1.02e-15 76.6
MsG0680034881.01.T01 MTR_6g087620 36.496 137 63 3 99 235 2 114 1.21e-15 75.5
MsG0680034881.01.T01 MTR_4g117780 31.884 207 111 8 27 232 11 188 2.01e-15 75.9
MsG0680034881.01.T01 MTR_4g094365 28.512 242 131 7 26 262 7 211 1.93e-14 73.2
MsG0680034881.01.T01 MTR_1g009110 32.298 161 96 5 22 177 9 161 2.04e-14 73.2
MsG0680034881.01.T01 MTR_8g013010 28.829 222 132 8 15 232 40 239 4.19e-14 71.6
MsG0680034881.01.T01 MTR_1g047080 30.601 183 94 8 64 236 1 160 6.63e-14 70.9
MsG0680034881.01.T01 MTR_4g117770 29.956 227 119 9 7 232 1 188 9.42e-14 70.9
MsG0680034881.01.T01 MTR_4g117810 29.956 227 119 9 7 232 1 188 9.42e-14 70.9
MsG0680034881.01.T01 MTR_5g068490 33.784 148 73 6 107 250 8 134 9.93e-14 70.1
MsG0680034881.01.T01 MTR_1g050542 29.075 227 129 8 6 232 40 234 1.48e-13 70.5
MsG0680034881.01.T01 MTR_5g086780 65.000 60 17 2 8 67 1 56 1.68e-13 68.9
MsG0680034881.01.T01 MTR_3g013740 28.226 248 137 10 2 230 14 239 1.87e-13 70.5
MsG0680034881.01.T01 MTR_4g133170 25.000 212 124 7 27 231 4 187 2.35e-13 70.1
MsG0680034881.01.T01 MTR_1g097560 28.270 237 130 9 1 228 5 210 2.75e-13 69.7
MsG0680034881.01.T01 MTR_3g006670 28.169 213 121 7 27 238 49 230 2.87e-13 69.7
MsG0680034881.01.T01 MTR_4g023080 27.935 247 127 11 8 250 1 200 4.70e-13 68.9
MsG0680034881.01.T01 MTR_8g433040 29.362 235 130 8 20 248 11 215 5.50e-13 68.6
MsG0680034881.01.T01 MTR_8g032380 29.362 235 130 8 20 248 11 215 5.50e-13 68.6
MsG0680034881.01.T01 MTR_5g057040 27.347 245 138 10 27 260 46 261 6.93e-13 69.3
MsG0680034881.01.T01 MTR_4g023090 29.808 208 121 8 26 232 15 198 9.61e-13 68.2
MsG0680034881.01.T01 MTR_1g097490 31.073 177 96 8 27 202 49 200 1.74e-12 67.4
MsG0680034881.01.T01 MTR_8g073550 26.887 212 131 6 26 235 29 218 4.83e-12 64.7
MsG0680034881.01.T01 MTR_5g057710 28.037 214 117 9 27 232 46 230 6.14e-12 65.9
MsG0680034881.01.T01 MTR_3g009310 27.700 213 123 10 27 232 42 230 6.36e-12 65.9
MsG0680034881.01.T01 MTR_5g011200 28.384 229 135 9 8 232 1 204 7.68e-12 65.5
MsG0680034881.01.T01 MTR_2g047832 27.897 233 134 9 5 231 6 210 9.10e-12 65.5
MsG0680034881.01.T01 MTR_4g021785 28.638 213 108 8 26 232 8 182 1.03e-11 65.1
MsG0680034881.01.T01 MTR_4g080200 30.939 181 89 8 27 205 3 149 2.12e-11 63.9
MsG0680034881.01.T01 MTR_3g007270 26.887 212 137 5 27 232 47 246 2.50e-11 63.9
MsG0680034881.01.T01 MTR_3g011100 28.311 219 119 8 27 232 235 428 3.99e-11 63.5
MsG0680034881.01.T01 MTR_5g057643 24.887 221 115 7 27 232 46 230 4.18e-11 62.4
MsG0680034881.01.T01 MTR_3g007430 26.957 230 133 9 35 257 51 252 4.28e-11 63.2
MsG0680034881.01.T01 MTR_7g056170 28.736 174 83 6 5 158 50 202 5.04e-11 63.2
MsG0680034881.01.T01 MTR_1g055135 29.384 211 115 9 26 231 12 193 5.74e-11 62.8
MsG0680034881.01.T01 MTR_4g023440 27.602 221 122 9 18 232 9 197 7.19e-11 62.4
MsG0680034881.01.T01 MTR_3g007410 29.808 208 112 10 35 233 60 242 7.30e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 56 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGAATAGAAAACTTCTTTA+TGG 0.132949 6:-96190415 None:intergenic
AAACGCTTTAAAGATTTAAA+AGG 0.200477 6:-96190254 None:intergenic
ATTCATGTTTGGAGCCCTTA+TGG 0.255955 6:+96190768 MsG0680034881.01.T01:CDS
ATAGCTTAAGAAATAACTTT+TGG 0.269465 6:+96190798 MsG0680034881.01.T01:CDS
GTGGAAGGATCATGCTTGTT+TGG 0.284124 6:-96190604 None:intergenic
TGGTAAGAAGTTTGAGAATA+AGG 0.292394 6:+96190442 MsG0680034881.01.T01:CDS
TGTGAAGAAGAAAGCCCTTC+TGG 0.321700 6:+96190904 MsG0680034881.01.T01:CDS
AGGTTGACATCCGTATTCTT+TGG 0.323572 6:+96190831 MsG0680034881.01.T01:CDS
ACGGAGCATCTCTCCTCTTA+AGG 0.342857 6:+96190381 MsG0680034881.01.T01:CDS
TGGAAGGATCATGCTTGTTT+GGG 0.360984 6:-96190603 None:intergenic
CATGATCCTTCCACCCAGTT+TGG 0.380113 6:+96190613 MsG0680034881.01.T01:CDS
AGAATAAGGTCAAATTAGAT+TGG 0.380739 6:+96190456 MsG0680034881.01.T01:CDS
AACTAGACTAGGAGCCAAAC+TGG 0.380763 6:-96190627 None:intergenic
TTCATGTTTGGAGCCCTTAT+GGG 0.383110 6:+96190769 MsG0680034881.01.T01:CDS
TATCTCAGACGATGAGGATA+TGG 0.383368 6:+96190655 MsG0680034881.01.T01:CDS
AGACGATGAGGATATGGATT+TGG 0.386491 6:+96190661 MsG0680034881.01.T01:CDS
ATCATGCTTGTTTGGGTAGG+TGG 0.395469 6:-96190596 None:intergenic
GTCCTCTACTTCTGAGGGTA+AGG 0.406537 6:-96190977 None:intergenic
AAGCCCTTCTGGATCATGTT+TGG 0.407834 6:+96190915 MsG0680034881.01.T01:CDS
CGGAGCATCTCTCCTCTTAA+GGG 0.409616 6:+96190382 MsG0680034881.01.T01:CDS
TTGAATGTGTTTGCAAATCA+TGG 0.420913 6:+96190276 MsG0680034881.01.T01:CDS
TAAGCTATATATCTCCCATA+AGG 0.425559 6:-96190783 None:intergenic
AACGCTTTAAAGATTTAAAA+GGG 0.449378 6:-96190253 None:intergenic
ACGGTTCGCAGATTCATGTT+TGG 0.456538 6:+96190757 MsG0680034881.01.T01:CDS
TCATGCTTGTTTGGGTAGGT+GGG 0.478028 6:-96190595 None:intergenic
GGCTTCAACGAAGAATGAAA+TGG 0.478343 6:+96190178 MsG0680034881.01.T01:CDS
TACATACATTCCAAAGAATA+CGG 0.486356 6:-96190841 None:intergenic
ACATGGATGGAGTGTGTCAT+TGG 0.492746 6:+96190878 MsG0680034881.01.T01:CDS
GGCTCATCTCAACACGATGA+TGG 0.512250 6:+96190139 MsG0680034881.01.T01:CDS
ATGGAGAAGTGAATATCATC+TGG 0.513516 6:-96190218 None:intergenic
AGGATCATGCTTGTTTGGGT+AGG 0.520952 6:-96190599 None:intergenic
ACACTCCATCCATGTAGACT+TGG 0.522257 6:-96190870 None:intergenic
TTTGGCTCCTAGTCTAGTTG+AGG 0.527993 6:+96190631 MsG0680034881.01.T01:CDS
AAGCTATATATCTCCCATAA+GGG 0.540514 6:-96190782 None:intergenic
TATTCTTTGGAATGTATGTA+CGG 0.546977 6:+96190844 MsG0680034881.01.T01:CDS
GTACGGCACCCAAGTCTACA+TGG 0.554754 6:+96190861 MsG0680034881.01.T01:CDS
CACTCCATCCATGTAGACTT+GGG 0.555541 6:-96190869 None:intergenic
TCCTCTACTTCTGAGGGTAA+GGG 0.557394 6:-96190976 None:intergenic
TAGGAGCCAAACTGGGTGGA+AGG 0.558076 6:-96190619 None:intergenic
AAACCAGTCCTCTACTTCTG+AGG 0.571783 6:-96190983 None:intergenic
GGAAGGTCATTCGTAATGTA+CGG 0.575587 6:+96190738 MsG0680034881.01.T01:CDS
AACCAGTCCTCTACTTCTGA+GGG 0.576782 6:-96190982 None:intergenic
GAGATAACCTCAACTAGACT+AGG 0.584233 6:-96190638 None:intergenic
GATGGAGAAGACTACAGCAT+TGG 0.592155 6:+96190157 MsG0680034881.01.T01:CDS
GGCACCCAAGTCTACATGGA+TGG 0.594373 6:+96190865 MsG0680034881.01.T01:CDS
ACCCTTACCCTCAGAAGTAG+AGG 0.594906 6:+96190975 MsG0680034881.01.T01:CDS
CATCGTTGTTCTTATTACGA+CGG 0.614675 6:+96190362 MsG0680034881.01.T01:CDS
ATGACCGTGTTATAGATGAC+TGG 0.621039 6:+96190717 MsG0680034881.01.T01:CDS
GATACCAAACATGATCCAGA+AGG 0.644084 6:-96190919 None:intergenic
ACTAGACTAGGAGCCAAACT+GGG 0.654727 6:-96190626 None:intergenic
ATGGTGGAAATATTGTATTG+TGG 0.658789 6:+96190570 MsG0680034881.01.T01:CDS
TGAGGTTATCTCAGACGATG+AGG 0.665933 6:+96190649 MsG0680034881.01.T01:CDS
CCGTGTTATAGATGACTGGA+AGG 0.672924 6:+96190721 MsG0680034881.01.T01:CDS
CCTTCCAGTCATCTATAACA+CGG 0.677183 6:-96190721 None:intergenic
AGACTAGGAGCCAAACTGGG+TGG 0.687024 6:-96190623 None:intergenic
ATACCAAACATGATCCAGAA+GGG 0.696088 6:-96190918 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATTTTTTATCATCTTCA+TGG + Chr6:96190686-96190705 MsG0680034881.01.T01:CDS 15.0%
!! GAAAAATACGAAAAATGAAA+TGG - Chr6:96190499-96190518 None:intergenic 20.0%
!!! AAACGCTTTAAAGATTTAAA+AGG - Chr6:96190257-96190276 None:intergenic 20.0%
!!! AACGCTTTAAAGATTTAAAA+GGG - Chr6:96190256-96190275 None:intergenic 20.0%
!!! ATAGCTTAAGAAATAACTTT+TGG + Chr6:96190798-96190817 MsG0680034881.01.T01:CDS 20.0%
!!! ATTTTTCGTATTTTTCGTTT+TGG + Chr6:96190503-96190522 MsG0680034881.01.T01:CDS 20.0%
! AGAATAAGGTCAAATTAGAT+TGG + Chr6:96190456-96190475 MsG0680034881.01.T01:CDS 25.0%
! GAGAATAGAAAACTTCTTTA+TGG - Chr6:96190418-96190437 None:intergenic 25.0%
! TACATACATTCCAAAGAATA+CGG - Chr6:96190844-96190863 None:intergenic 25.0%
!! TATAATTTTTCTACTGGTGA+TGG + Chr6:96190551-96190570 MsG0680034881.01.T01:CDS 25.0%
!! TGTCTGTATAATTTTTCTAC+TGG + Chr6:96190545-96190564 MsG0680034881.01.T01:CDS 25.0%
!! TTTTATCATCTTCATGGATT+TGG + Chr6:96190692-96190711 MsG0680034881.01.T01:CDS 25.0%
!!! TATTCTTTGGAATGTATGTA+CGG + Chr6:96190844-96190863 MsG0680034881.01.T01:CDS 25.0%
!!! TTTCGTTTTGGTAGTATTAA+TGG + Chr6:96190515-96190534 MsG0680034881.01.T01:CDS 25.0%
AAGCTATATATCTCCCATAA+GGG - Chr6:96190785-96190804 None:intergenic 30.0%
TAAGCTATATATCTCCCATA+AGG - Chr6:96190786-96190805 None:intergenic 30.0%
TGGTAAGAAGTTTGAGAATA+AGG + Chr6:96190442-96190461 MsG0680034881.01.T01:CDS 30.0%
TTGAATGTGTTTGCAAATCA+TGG + Chr6:96190276-96190295 MsG0680034881.01.T01:CDS 30.0%
! ATCGTTTTACTTTAGCAATG+AGG + Chr6:96190939-96190958 MsG0680034881.01.T01:CDS 30.0%
! ATGGTGGAAATATTGTATTG+TGG + Chr6:96190570-96190589 MsG0680034881.01.T01:CDS 30.0%
! GAAGTTTTCTATTCTCTTTC+TGG + Chr6:96190422-96190441 MsG0680034881.01.T01:CDS 30.0%
!! AAAAGGGAGTTTTGATAGAA+TGG - Chr6:96190240-96190259 None:intergenic 30.0%
ATACCAAACATGATCCAGAA+GGG - Chr6:96190921-96190940 None:intergenic 35.0%
ATGGAGAAGTGAATATCATC+TGG - Chr6:96190221-96190240 None:intergenic 35.0%
CATCGTTGTTCTTATTACGA+CGG + Chr6:96190362-96190381 MsG0680034881.01.T01:CDS 35.0%
!! TAACTTTTGGAGAGAACTTG+AGG + Chr6:96190811-96190830 MsG0680034881.01.T01:CDS 35.0%
!!! AATTTTTCTACTGGTGATGG+TGG + Chr6:96190554-96190573 MsG0680034881.01.T01:CDS 35.0%
AGACGATGAGGATATGGATT+TGG + Chr6:96190661-96190680 MsG0680034881.01.T01:CDS 40.0%
AGGTTGACATCCGTATTCTT+TGG + Chr6:96190831-96190850 MsG0680034881.01.T01:CDS 40.0%
ATGACCGTGTTATAGATGAC+TGG + Chr6:96190717-96190736 MsG0680034881.01.T01:CDS 40.0%
CCTTCCAGTCATCTATAACA+CGG - Chr6:96190724-96190743 None:intergenic 40.0%
GAGATAACCTCAACTAGACT+AGG - Chr6:96190641-96190660 None:intergenic 40.0%
GATACCAAACATGATCCAGA+AGG - Chr6:96190922-96190941 None:intergenic 40.0%
GGAAGGTCATTCGTAATGTA+CGG + Chr6:96190738-96190757 MsG0680034881.01.T01:CDS 40.0%
GGCTTCAACGAAGAATGAAA+TGG + Chr6:96190178-96190197 MsG0680034881.01.T01:CDS 40.0%
TATCTCAGACGATGAGGATA+TGG + Chr6:96190655-96190674 MsG0680034881.01.T01:CDS 40.0%
TGGAAGGATCATGCTTGTTT+GGG - Chr6:96190606-96190625 None:intergenic 40.0%
! ATTCATGTTTGGAGCCCTTA+TGG + Chr6:96190768-96190787 MsG0680034881.01.T01:CDS 40.0%
! GGTTTTCAGAATCCCTTAAG+AGG - Chr6:96190397-96190416 None:intergenic 40.0%
! TTCATGTTTGGAGCCCTTAT+GGG + Chr6:96190769-96190788 MsG0680034881.01.T01:CDS 40.0%
AACTAGACTAGGAGCCAAAC+TGG - Chr6:96190630-96190649 None:intergenic 45.0%
AAGCCCTTCTGGATCATGTT+TGG + Chr6:96190915-96190934 MsG0680034881.01.T01:CDS 45.0%
ACACTCCATCCATGTAGACT+TGG - Chr6:96190873-96190892 None:intergenic 45.0%
ACATGGATGGAGTGTGTCAT+TGG + Chr6:96190878-96190897 MsG0680034881.01.T01:CDS 45.0%
ACGGTTCGCAGATTCATGTT+TGG + Chr6:96190757-96190776 MsG0680034881.01.T01:CDS 45.0%
ACTAGACTAGGAGCCAAACT+GGG - Chr6:96190629-96190648 None:intergenic 45.0%
AGGATCATGCTTGTTTGGGT+AGG - Chr6:96190602-96190621 None:intergenic 45.0%
ATCATGCTTGTTTGGGTAGG+TGG - Chr6:96190599-96190618 None:intergenic 45.0%
CACTCCATCCATGTAGACTT+GGG - Chr6:96190872-96190891 None:intergenic 45.0%
CCGTGTTATAGATGACTGGA+AGG + Chr6:96190721-96190740 MsG0680034881.01.T01:CDS 45.0%
GATGGAGAAGACTACAGCAT+TGG + Chr6:96190157-96190176 MsG0680034881.01.T01:CDS 45.0%
GTGGAAGGATCATGCTTGTT+TGG - Chr6:96190607-96190626 None:intergenic 45.0%
TCATGCTTGTTTGGGTAGGT+GGG - Chr6:96190598-96190617 None:intergenic 45.0%
TGAGGTTATCTCAGACGATG+AGG + Chr6:96190649-96190668 MsG0680034881.01.T01:CDS 45.0%
TGTGAAGAAGAAAGCCCTTC+TGG + Chr6:96190904-96190923 MsG0680034881.01.T01:CDS 45.0%
! TTTGGCTCCTAGTCTAGTTG+AGG + Chr6:96190631-96190650 MsG0680034881.01.T01:CDS 45.0%
CATGATCCTTCCACCCAGTT+TGG + Chr6:96190613-96190632 MsG0680034881.01.T01:CDS 50.0%
GGCTCATCTCAACACGATGA+TGG + Chr6:96190139-96190158 MsG0680034881.01.T01:CDS 50.0%
! ACGGAGCATCTCTCCTCTTA+AGG + Chr6:96190381-96190400 MsG0680034881.01.T01:CDS 50.0%
! CGGAGCATCTCTCCTCTTAA+GGG + Chr6:96190382-96190401 MsG0680034881.01.T01:CDS 50.0%
AGACTAGGAGCCAAACTGGG+TGG - Chr6:96190626-96190645 None:intergenic 55.0%
GGCACCCAAGTCTACATGGA+TGG + Chr6:96190865-96190884 MsG0680034881.01.T01:CDS 55.0%
GTACGGCACCCAAGTCTACA+TGG + Chr6:96190861-96190880 MsG0680034881.01.T01:CDS 55.0%
TAGGAGCCAAACTGGGTGGA+AGG - Chr6:96190622-96190641 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 96190137 96190994 96190137 ID=MsG0680034881.01;Name=MsG0680034881.01
Chr6 mRNA 96190137 96190994 96190137 ID=MsG0680034881.01.T01;Parent=MsG0680034881.01;Name=MsG0680034881.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|285
Chr6 exon 96190137 96190994 96190137 ID=MsG0680034881.01.T01:exon:6725;Parent=MsG0680034881.01.T01
Chr6 CDS 96190137 96190994 96190137 ID=MsG0680034881.01.T01:cds;Parent=MsG0680034881.01.T01
Gene Sequence

>MsG0680034881.01.T01

ATGGCTCATCTCAACACGATGATGGAGAAGACTACAGCATTGGCTTCAACGAAGAATGAAATGGTTAGTAACAACTATATACCAGATGATATTCACTTCTCCATTCTATCAAAACTCCCTTTTAAATCTTTAAAGCGTTTTGAATGTGTTTGCAAATCATGGTCTCTCTTGTTTGAAAATACTTATTTTATGAATATGTTTCGCAATGATCTCGTAACTAACTCTCATCGTTGTTCTTATTACGACGGAGCATCTCTCCTCTTAAGGGATTCTGAAAACCATAAAGAAGTTTTCTATTCTCTTTCTGGTAAGAAGTTTGAGAATAAGGTCAAATTAGATTGGTTTAATTCATCTCAAGACCATTTCATTTTTCGTATTTTTCGTTTTGGTAGTATTAATGGCATACTTTGTCTGTATAATTTTTCTACTGGTGATGGTGGAAATATTGTATTGTGGAACCCACCTACCCAAACAAGCATGATCCTTCCACCCAGTTTGGCTCCTAGTCTAGTTGAGGTTATCTCAGACGATGAGGATATGGATTTGGAGTATATTTTTTATCATCTTCATGGATTTGGTTATGACCGTGTTATAGATGACTGGAAGGTCATTCGTAATGTACGGTTCGCAGATTCATGTTTGGAGCCCTTATGGGAGATATATAGCTTAAGAAATAACTTTTGGAGAGAACTTGAGGTTGACATCCGTATTCTTTGGAATGTATGTACGGCACCCAAGTCTACATGGATGGAGTGTGTCATTGGTTGTGTGAAGAAGAAAGCCCTTCTGGATCATGTTTGGTATCGTTTTACTTTAGCAATGAGGTGTTCTTCACAACACCCTTACCCTCAGAAGTAG
Protein sequence

>MsG0680034881.01.T01

MAHLNTMMEKTTALASTKNEMVSNNYIPDDIHFSILSKLPFKSLKRFECVCKSWSLLFENTYFMNMFRNDLVTNSHRCSYYDGASLLLRDSENHKEVFYSLSGKKFENKVKLDWFNSSQDHFIFRIFRFGSINGILCLYNFSTGDGGNIVLWNPPTQTSMILPPSLAPSLVEVISDDEDMDLEYIFYHLHGFGYDRVIDDWKVIRNVRFADSCLEPLWEIYSLRNNFWRELEVDIRILWNVCTAPKSTWMECVIGCVKKKALLDHVWYRFTLAMRCSSQHPYPQK*