AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046936.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046936.01.T01 MTR_4g117550 71.357 398 59 3 1 367 1 374 0.0 552
MsG0880046936.01.T01 MTR_0129s0070 62.250 400 115 3 1 367 1 397 9.42e-172 484
MsG0880046936.01.T01 MTR_0129s0120 60.500 400 122 3 1 367 1 397 1.02e-164 466
MsG0880046936.01.T01 MTR_0129s0050 55.502 418 124 10 1 366 1 408 2.27e-134 390
MsG0880046936.01.T01 MTR_8g063440 46.547 391 167 7 9 367 4 384 1.38e-111 331
MsG0880046936.01.T01 MTR_2g038680 45.238 420 162 12 3 365 2 410 4.74e-104 312
MsG0880046936.01.T01 MTR_4g102410 44.146 410 178 10 1 364 44 448 7.02e-102 308
MsG0880046936.01.T01 MTR_5g097330 45.946 407 162 12 8 366 7 403 6.52e-100 301
MsG0880046936.01.T01 MTR_4g014370 41.966 417 171 10 10 366 4 409 9.07e-99 299
MsG0880046936.01.T01 MTR_1g007450 45.833 384 165 6 8 352 3 382 9.53e-99 299
MsG0880046936.01.T01 MTR_2g022000 44.819 415 164 13 1 358 1 407 2.58e-98 298
MsG0880046936.01.T01 MTR_2g021800 43.794 427 170 14 1 367 1 417 1.34e-97 296
MsG0880046936.01.T01 MTR_6g086825 44.802 404 164 10 14 367 67 461 2.62e-97 297
MsG0880046936.01.T01 MTR_2g021770 43.210 405 180 7 11 367 6 408 4.15e-95 290
MsG0880046936.01.T01 MTR_5g096630 43.447 412 174 11 8 366 9 414 2.26e-94 288
MsG0880046936.01.T01 MTR_5g037680 43.250 400 154 12 12 360 3 380 1.34e-92 285
MsG0880046936.01.T01 MTR_6g079770 42.959 419 180 12 1 364 1 415 2.01e-92 283
MsG0880046936.01.T01 MTR_8g464770 43.750 384 169 10 12 362 7 376 6.58e-92 280
MsG0880046936.01.T01 MTR_6g080680 43.204 412 159 12 1 360 1 389 1.97e-90 277
MsG0880046936.01.T01 MTR_6g079810 43.914 419 169 14 1 364 54 461 6.45e-90 278
MsG0880046936.01.T01 MTR_0134s0060 42.439 410 181 8 9 365 4 411 1.68e-89 275
MsG0880046936.01.T01 MTR_2g066150 42.432 370 190 9 9 367 4 361 7.70e-89 272
MsG0880046936.01.T01 MTR_6g090180 43.137 408 182 10 1 362 31 434 1.36e-88 274
MsG0880046936.01.T01 MTR_8g041270 43.980 407 168 12 11 366 9 406 2.25e-88 272
MsG0880046936.01.T01 MTR_2g104340 43.921 403 171 13 8 366 5 396 2.33e-88 272
MsG0880046936.01.T01 MTR_0054s0090 41.727 417 183 10 1 367 1 407 2.59e-88 272
MsG0880046936.01.T01 MTR_7g069550 42.647 408 179 15 1 366 1 395 4.89e-88 271
MsG0880046936.01.T01 MTR_8g012090 45.380 368 119 7 9 335 8 334 5.21e-88 269
MsG0880046936.01.T01 MTR_2g066170 42.356 399 175 13 9 367 4 387 6.28e-88 270
MsG0880046936.01.T01 MTR_2g021950 41.832 404 182 11 1 362 1 393 9.23e-88 270
MsG0880046936.01.T01 MTR_0054s0100 41.007 417 188 9 1 367 1 409 3.43e-86 266
MsG0880046936.01.T01 MTR_1g007430 47.077 325 137 7 28 324 7 324 3.67e-86 264
MsG0880046936.01.T01 MTR_3g011120 43.272 379 170 11 8 355 5 369 1.10e-85 264
MsG0880046936.01.T01 MTR_4g055820 44.413 358 153 8 6 320 3 357 2.22e-85 263
MsG0880046936.01.T01 MTR_5g097350 42.432 403 171 11 1 362 1 383 4.39e-85 263
MsG0880046936.01.T01 MTR_0831s0010 39.948 383 190 6 14 365 47 420 6.37e-85 264
MsG0880046936.01.T01 MTR_8g467670 42.408 382 176 12 16 367 13 380 1.17e-84 262
MsG0880046936.01.T01 MTR_5g059430 39.568 417 185 13 7 364 16 424 7.45e-84 261
MsG0880046936.01.T01 MTR_6g461660 41.353 399 186 8 8 366 10 400 1.30e-83 260
MsG0880046936.01.T01 MTR_5g096910 40.099 404 177 12 8 365 8 392 3.47e-83 258
MsG0880046936.01.T01 MTR_8g033140 40.095 419 187 11 8 367 6 419 5.89e-83 259
MsG0880046936.01.T01 MTR_2g104280 42.054 409 179 13 8 366 5 405 8.08e-83 258
MsG0880046936.01.T01 MTR_7g017130 40.796 402 193 8 2 366 4 397 1.05e-82 257
MsG0880046936.01.T01 MTR_5g069390 40.394 406 184 10 11 366 20 417 1.47e-82 260
MsG0880046936.01.T01 MTR_1g007410 45.363 399 157 17 8 358 13 398 1.56e-82 257
MsG0880046936.01.T01 MTR_7g017040 40.943 403 177 9 12 367 11 399 4.13e-82 256
MsG0880046936.01.T01 MTR_5g072060 46.132 349 161 10 15 341 4 347 3.07e-81 260
MsG0880046936.01.T01 MTR_7g111700 44.020 393 167 16 1 367 1 366 7.37e-81 251
MsG0880046936.01.T01 MTR_5g097220 40.714 420 183 13 1 366 1 408 1.20e-80 252
MsG0880046936.01.T01 MTR_2g066200 39.901 406 179 12 8 367 3 389 1.30e-80 252
MsG0880046936.01.T01 MTR_4g130330 39.901 406 173 11 14 366 4 391 3.55e-80 251
MsG0880046936.01.T01 MTR_7g017170 39.614 414 193 9 2 366 4 409 1.25e-79 250
MsG0880046936.01.T01 MTR_4g028680 39.750 400 184 8 14 367 13 401 1.52e-78 247
MsG0880046936.01.T01 MTR_2g065860 39.401 401 182 12 11 367 6 389 5.92e-78 245
MsG0880046936.01.T01 MTR_8g064890 39.386 391 193 10 11 367 9 389 8.08e-78 244
MsG0880046936.01.T01 MTR_1g050308 41.644 365 165 10 9 367 26 348 2.91e-77 242
MsG0880046936.01.T01 MTR_1g053510 39.494 395 189 12 14 362 12 402 3.75e-77 243
MsG0880046936.01.T01 MTR_2g034700 40.000 385 183 10 9 362 5 372 5.49e-77 242
MsG0880046936.01.T01 MTR_4g028510 40.964 415 165 13 11 366 9 402 8.86e-77 244
MsG0880046936.01.T01 MTR_5g097680 39.679 436 184 14 1 366 1 427 8.99e-76 240
MsG0880046936.01.T01 MTR_4g028660 38.750 400 177 10 14 362 13 395 9.01e-76 239
MsG0880046936.01.T01 MTR_4g027450 38.750 400 177 10 14 362 13 395 9.01e-76 239
MsG0880046936.01.T01 MTR_2g065840 38.670 406 185 13 8 367 3 390 2.96e-75 238
MsG0880046936.01.T01 MTR_7g079640 41.361 382 158 14 1 344 1 354 3.33e-74 235
MsG0880046936.01.T01 MTR_8g013000 40.845 355 170 6 8 324 1 353 9.55e-74 234
MsG0880046936.01.T01 MTR_8g046310 39.547 397 172 15 8 362 4 374 2.06e-73 233
MsG0880046936.01.T01 MTR_6g478230 38.537 410 171 8 14 367 24 408 4.30e-73 233
MsG0880046936.01.T01 MTR_8g046200 37.097 434 181 16 5 362 1 418 5.72e-73 233
MsG0880046936.01.T01 MTR_8g046220 37.650 417 186 13 5 362 1 402 3.23e-72 231
MsG0880046936.01.T01 MTR_8g064790 38.630 365 188 8 9 367 4 338 4.32e-72 228
MsG0880046936.01.T01 MTR_2g064950 40.253 395 177 13 8 357 1 381 1.30e-71 229
MsG0880046936.01.T01 MTR_8g039500 39.623 371 152 10 9 335 4 346 3.19e-71 226
MsG0880046936.01.T01 MTR_8g033090 38.329 407 192 13 14 366 11 412 1.49e-69 224
MsG0880046936.01.T01 MTR_8g033110 38.835 412 186 15 14 366 11 415 2.16e-69 224
MsG0880046936.01.T01 MTR_5g091140 38.303 389 170 9 2 345 33 396 2.65e-68 221
MsG0880046936.01.T01 MTR_5g024260 38.734 395 176 15 1 344 5 384 6.73e-68 219
MsG0880046936.01.T01 MTR_4g028750 39.583 384 172 11 8 344 6 376 1.16e-65 213
MsG0880046936.01.T01 MTR_1g098180 37.594 399 177 12 1 363 1 363 3.06e-65 211
MsG0880046936.01.T01 MTR_4g007940 37.463 339 167 7 67 363 4 339 1.23e-63 206
MsG0880046936.01.T01 MTR_8g018110 37.626 396 195 15 14 366 11 397 1.80e-63 207
MsG0880046936.01.T01 MTR_8g032980 35.566 433 195 16 6 367 3 422 1.89e-63 208
MsG0880046936.01.T01 MTR_6g090250 37.975 395 175 10 35 362 29 420 4.57e-63 207
MsG0880046936.01.T01 MTR_5g074800 40.857 350 158 14 56 366 1 340 2.34e-62 203
MsG0880046936.01.T01 MTR_5g022850 36.111 396 159 9 12 366 11 353 3.61e-62 203
MsG0880046936.01.T01 MTR_5g092850 36.616 396 190 13 16 362 15 398 2.34e-61 202
MsG0880046936.01.T01 MTR_1g050312 41.096 292 128 8 91 358 16 287 9.34e-60 195
MsG0880046936.01.T01 MTR_8g063330 37.500 352 170 11 11 321 6 348 1.11e-59 200
MsG0880046936.01.T01 MTR_8g036970 36.512 367 140 10 23 353 4 313 7.80e-59 195
MsG0880046936.01.T01 MTR_6g078010 37.853 354 150 9 54 361 1 330 4.05e-58 192
MsG0880046936.01.T01 MTR_5g074850 37.975 395 179 17 5 346 23 404 1.78e-56 198
MsG0880046936.01.T01 MTR_7g016930 39.548 354 146 10 11 326 10 333 1.82e-56 187
MsG0880046936.01.T01 MTR_5g064700 36.412 379 162 14 56 366 1 368 1.77e-53 181
MsG0880046936.01.T01 MTR_6g070880 42.917 240 115 7 12 235 27 260 2.07e-52 175
MsG0880046936.01.T01 MTR_6g071420 42.917 240 115 7 12 235 27 260 2.07e-52 175
MsG0880046936.01.T01 MTR_5g075370 36.573 391 175 18 14 346 5 380 1.10e-51 184
MsG0880046936.01.T01 MTR_6g079790 36.137 321 137 7 103 367 2 310 5.06e-51 172
MsG0880046936.01.T01 MTR_8g073570 50.286 175 82 2 8 182 1 170 1.04e-50 167
MsG0880046936.01.T01 MTR_5g096900 40.549 328 124 11 12 289 7 313 1.26e-50 172
MsG0880046936.01.T01 MTR_5g068490 37.374 297 131 9 100 367 8 278 1.80e-50 170
MsG0880046936.01.T01 MTR_1g076810 45.344 247 114 10 1 239 1 234 2.91e-48 173
MsG0880046936.01.T01 MTR_6g004450 43.777 233 86 6 168 365 46 268 7.68e-48 163
MsG0880046936.01.T01 MTR_5g097820 43.277 238 115 8 3 231 9 235 8.26e-48 163
MsG0880046936.01.T01 MTR_5g092860 35.043 351 166 13 35 343 20 350 9.70e-48 166
MsG0880046936.01.T01 MTR_5g037190 46.396 222 111 7 1 218 1 218 2.87e-47 161
MsG0880046936.01.T01 MTR_5g043605 32.828 396 147 13 13 366 21 339 5.30e-47 164
MsG0880046936.01.T01 MTR_6g087620 35.577 312 122 10 91 356 1 279 9.87e-47 160
MsG0880046936.01.T01 MTR_2g021920 43.038 237 86 7 171 362 37 269 1.18e-46 160
MsG0880046936.01.T01 MTR_8g063310 30.905 398 207 12 8 358 13 389 2.53e-46 163
MsG0880046936.01.T01 MTR_7g023800 38.050 318 165 12 56 357 1 302 4.44e-46 160
MsG0880046936.01.T01 MTR_5g076490 37.260 365 148 14 54 366 1 336 5.30e-46 160
MsG0880046936.01.T01 MTR_6g465360 47.642 212 93 8 2 200 47 253 3.86e-45 157
MsG0880046936.01.T01 MTR_8g063370 29.688 384 209 10 15 358 22 384 4.16e-45 159
MsG0880046936.01.T01 MTR_5g086590 40.833 240 99 6 138 348 13 238 1.82e-44 153
MsG0880046936.01.T01 MTR_5g023970 45.361 194 71 3 207 366 4 196 2.08e-44 152
MsG0880046936.01.T01 MTR_5g098950 45.312 192 91 4 140 324 90 274 1.17e-41 147
MsG0880046936.01.T01 MTR_7g104710 35.583 326 124 10 1 281 1 285 6.46e-41 146
MsG0880046936.01.T01 MTR_2g104260 43.697 238 113 7 8 232 5 234 1.33e-39 142
MsG0880046936.01.T01 MTR_8g033000 32.181 376 179 14 54 366 1 363 1.51e-39 144
MsG0880046936.01.T01 MTR_4g014370 41.081 185 78 4 213 366 153 337 7.79e-38 139
MsG0880046936.01.T01 MTR_4g014370 55.906 127 53 2 10 134 15 140 1.26e-37 138
MsG0880046936.01.T01 MTR_8g063350 28.571 392 175 11 9 356 6 336 1.43e-37 138
MsG0880046936.01.T01 MTR_5g086780 39.216 204 84 2 139 342 65 228 3.73e-37 134
MsG0880046936.01.T01 MTR_5g086780 63.492 63 19 2 1 60 1 62 8.36e-15 73.6
MsG0880046936.01.T01 MTR_6g090260 40.860 186 73 3 214 362 75 260 1.61e-36 133
MsG0880046936.01.T01 MTR_6g090260 55.072 69 28 2 1 66 1 69 2.53e-14 72.8
MsG0880046936.01.T01 MTR_4g016000 39.234 209 97 6 143 333 2 198 2.34e-36 131
MsG0880046936.01.T01 MTR_7g019250 41.000 200 83 4 170 343 223 413 6.95e-36 136
MsG0880046936.01.T01 MTR_5g097850 34.630 257 131 7 120 365 14 244 1.39e-35 130
MsG0880046936.01.T01 MTR_5g066420 50.000 122 60 1 235 355 28 149 2.25e-32 119
MsG0880046936.01.T01 MTR_6g079730 50.400 125 62 0 238 362 22 146 2.33e-31 116
MsG0880046936.01.T01 MTR_5g094350 38.494 239 114 8 13 237 9 228 2.44e-31 120
MsG0880046936.01.T01 MTR_5g036250 64.634 82 28 1 277 358 23 103 2.49e-30 114
MsG0880046936.01.T01 MTR_1g115205 36.400 250 103 9 1 238 17 222 4.14e-30 116
MsG0880046936.01.T01 MTR_3g018750 35.000 240 113 6 170 366 2 241 5.34e-30 115
MsG0880046936.01.T01 MTR_5g096920 42.105 152 79 3 214 364 7 150 9.03e-30 112
MsG0880046936.01.T01 MTR_5g097660 51.754 114 54 1 250 362 3 116 1.12e-29 110
MsG0880046936.01.T01 MTR_8g035690 36.032 247 104 9 171 365 58 302 2.71e-29 115
MsG0880046936.01.T01 MTR_2g022020 47.458 118 60 1 245 362 1 116 3.98e-28 106
MsG0880046936.01.T01 MTR_3g019290 34.300 207 97 3 14 220 11 178 7.93e-27 105
MsG0880046936.01.T01 MTR_3g030620 48.855 131 61 4 239 364 51 180 8.35e-27 105
MsG0880046936.01.T01 MTR_6g009350 35.961 203 77 5 168 329 5 195 1.46e-26 105
MsG0880046936.01.T01 MTR_5g096070 41.111 180 64 7 8 179 14 159 4.73e-25 100
MsG0880046936.01.T01 MTR_5g097650 41.611 149 72 4 10 155 13 149 1.24e-24 99.0
MsG0880046936.01.T01 MTR_3g047580 40.136 147 57 6 11 152 7 127 1.54e-23 94.7
MsG0880046936.01.T01 MTR_1g047080 29.321 324 177 14 54 366 1 283 1.85e-22 96.3
MsG0880046936.01.T01 MTR_5g097540 34.807 181 80 5 207 352 90 267 3.95e-22 95.5
MsG0880046936.01.T01 MTR_7g017120 35.897 195 86 5 208 366 75 266 3.03e-20 89.7
MsG0880046936.01.T01 MTR_8g041440 42.609 115 39 3 8 115 6 100 9.43e-20 84.3
MsG0880046936.01.T01 MTR_7g017150 35.385 195 87 5 208 366 76 267 2.03e-19 87.4
MsG0880046936.01.T01 MTR_4g023080 26.250 400 202 17 11 367 3 352 4.51e-19 87.8
MsG0880046936.01.T01 MTR_4g021785 26.913 379 217 14 16 367 8 353 8.32e-19 87.0
MsG0880046936.01.T01 MTR_3g097190 39.837 123 27 1 217 339 6 81 1.53e-18 82.8
MsG0880046936.01.T01 MTR_1g050305 54.321 81 32 2 269 344 23 103 3.73e-18 80.9
MsG0880046936.01.T01 MTR_5g096050 53.333 75 35 0 235 309 35 109 2.77e-17 77.4
MsG0880046936.01.T01 MTR_5g049560 39.333 150 71 7 54 186 1 147 9.17e-17 79.7
MsG0880046936.01.T01 MTR_1g097490 27.401 354 199 15 9 334 41 364 2.12e-16 80.1
MsG0880046936.01.T01 MTR_1g097560 25.000 376 229 14 8 360 27 372 1.21e-15 77.8
MsG0880046936.01.T01 MTR_3g034160 25.700 393 228 14 11 364 3 370 6.66e-15 75.5
MsG0880046936.01.T01 MTR_4g133170 23.896 385 223 16 17 360 4 359 1.73e-14 74.7
MsG0880046936.01.T01 MTR_1g050542 23.947 380 219 11 17 360 58 403 5.96e-14 73.2
MsG0880046936.01.T01 MTR_2g021155 25.806 310 175 11 20 301 39 321 8.54e-14 73.2
MsG0880046936.01.T01 MTR_3g058470 26.756 299 169 13 17 299 37 301 1.17e-13 72.0
MsG0880046936.01.T01 MTR_2g046900 29.545 220 112 9 17 227 28 213 2.81e-13 70.9
MsG0880046936.01.T01 MTR_4g094400 59.649 57 23 0 243 299 55 111 4.45e-13 66.2
MsG0880046936.01.T01 MTR_4g117870 28.279 244 143 10 126 364 46 262 9.24e-13 68.2
MsG0880046936.01.T01 MTR_2g028110 23.796 353 175 14 17 317 4 314 9.61e-13 69.3
MsG0880046936.01.T01 MTR_1g068835 24.691 324 171 13 17 302 4 292 1.50e-12 68.6
MsG0880046936.01.T01 MTR_8g041490 38.462 104 35 3 5 108 3 77 1.50e-12 63.9
MsG0880046936.01.T01 MTR_2g024100 24.000 350 164 15 31 317 21 331 1.71e-12 68.6
MsG0880046936.01.T01 MTR_0667s0010 22.674 344 172 14 13 301 2 306 2.10e-12 68.2
MsG0880046936.01.T01 MTR_6g087660 45.000 80 43 1 1 79 1 80 3.10e-12 65.5
MsG0880046936.01.T01 MTR_5g022420 22.727 374 204 14 5 336 15 345 3.41e-12 67.4
MsG0880046936.01.T01 MTR_6g027910 24.719 356 185 16 20 329 12 330 3.76e-12 67.4
MsG0880046936.01.T01 MTR_3g024040 28.713 303 155 16 25 302 39 305 4.43e-12 67.0
MsG0880046936.01.T01 MTR_3g034130 26.230 244 147 9 12 254 4 215 4.84e-12 67.0
MsG0880046936.01.T01 MTR_2g028140 22.443 352 179 14 13 301 1 321 6.76e-12 66.6
MsG0880046936.01.T01 MTR_3g013740 26.180 233 134 8 12 232 39 245 1.66e-11 65.5
MsG0880046936.01.T01 MTR_4g094365 22.739 387 229 10 16 364 7 361 2.71e-11 64.7
MsG0880046936.01.T01 MTR_0115s0020 31.532 222 121 15 16 228 9 208 2.74e-11 64.7
MsG0880046936.01.T01 MTR_1g022200 28.910 211 108 8 20 224 18 192 2.86e-11 64.7
MsG0880046936.01.T01 MTR_3g006670 27.419 310 178 12 17 301 49 336 4.43e-11 64.3
MsG0880046936.01.T01 MTR_2g021150 24.847 326 169 14 17 301 14 304 4.57e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046936.01.T01 AT4G12560 26.168 214 128 8 17 228 4 189 1.68e-11 65.5
MsG0880046936.01.T01 AT4G12560 26.168 214 128 8 17 228 4 189 1.68e-11 65.5
MsG0880046936.01.T01 AT4G12560 26.168 214 128 8 17 228 4 189 1.68e-11 65.5
MsG0880046936.01.T01 AT3G16210 28.000 175 100 5 13 187 1 149 1.72e-11 65.5

Find 75 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGTGTTATACTTGCTTTC+TGG 0.163821 8:-80175031 MsG0880046936.01.T01:CDS
ACTGAGGAGCTTGGTGTTAA+AGG 0.215377 8:-80174191 MsG0880046936.01.T01:CDS
CTTGACTATATTTATCTTCA+TGG 0.238560 8:-80174803 MsG0880046936.01.T01:CDS
TATATTTATCTTCATGGATT+TGG 0.252301 8:-80174797 MsG0880046936.01.T01:CDS
ACATGGTGATTTGGAAAATA+TGG 0.265633 8:+80175049 None:intergenic
TTCAGTCGTAACATGATCTA+TGG 0.269409 8:+80175285 None:intergenic
TGGACTAAACTCTTCATTCT+TGG 0.274555 8:-80174320 MsG0880046936.01.T01:CDS
CTTGGCATTGAACATCCTAT+TGG 0.291282 8:-80174290 MsG0880046936.01.T01:CDS
ATATTGAAGTTGGGATAGTA+TGG 0.316710 8:-80174697 MsG0880046936.01.T01:CDS
ATTGAAAATTATATTGAAGT+TGG 0.317159 8:-80174707 MsG0880046936.01.T01:CDS
CTAGATATGCCTCAATGTTA+TGG 0.333387 8:-80174611 MsG0880046936.01.T01:CDS
TACACGGATGCCTTCTGATA+TGG 0.341698 8:-80174477 MsG0880046936.01.T01:CDS
CTGAGGAGCTTGGTGTTAAA+GGG 0.350114 8:-80174190 MsG0880046936.01.T01:CDS
TTGAAAATTATATTGAAGTT+GGG 0.356748 8:-80174706 MsG0880046936.01.T01:CDS
CACCAAGCTCCTCAGTCATC+TGG 0.358485 8:+80174198 None:intergenic
CCATTACTTCCCTTTCCAAT+AGG 0.362020 8:+80174275 None:intergenic
GGGTTCCATAACACAACATT+AGG 0.366401 8:+80174887 None:intergenic
AAGGAAAGTCTTCTTCCTCT+TGG 0.377051 8:-80174137 MsG0880046936.01.T01:CDS
ACATTCAAATTGTCAATAGT+TGG 0.381127 8:+80174866 None:intergenic
AGAATAAGGTCAAATTAGAT+TGG 0.382141 8:-80174997 MsG0880046936.01.T01:CDS
GGGTGTCATGTCGTTACATA+TGG 0.383673 8:-80174676 MsG0880046936.01.T01:CDS
TTCATTCTTGGACCCTTACT+TGG 0.384139 8:-80174308 MsG0880046936.01.T01:CDS
CAGACAATGCCGTTAATAAC+AGG 0.385505 8:+80174929 None:intergenic
AACCGTTTCAAAGATTTAAG+AGG 0.387329 8:+80175208 None:intergenic
AACTCGGAGTGAAGGAATCT+TGG 0.396768 8:-80174340 MsG0880046936.01.T01:CDS
GATTGTGTTGAGATACTTGA+TGG 0.399469 8:-80174435 MsG0880046936.01.T01:CDS
GATTGGCCATTCTCTTGAAA+TGG 0.402721 8:+80174968 None:intergenic
TAGATATGCCTCAATGTTAT+GGG 0.413458 8:-80174610 MsG0880046936.01.T01:CDS
AAGTATAACACATGGTGATT+TGG 0.414679 8:+80175040 None:intergenic
CCACCTCCATTTCAAGAGAA+TGG 0.425367 8:-80174974 MsG0880046936.01.T01:CDS
CATTCAAATTGTCAATAGTT+GGG 0.433745 8:+80174867 None:intergenic
AGGATGTTCAATGCCAAGTA+AGG 0.438687 8:+80174295 None:intergenic
TCTCAACACAATCACAAAAT+CGG 0.441386 8:+80174445 None:intergenic
TGCCTCTTAAATCTTTGAAA+CGG 0.468477 8:-80175210 MsG0880046936.01.T01:CDS
AGGGGAAGCGTATTGTTGTC+AGG 0.482447 8:-80174171 MsG0880046936.01.T01:CDS
ATGGTATTAAATCAGTCCAT+TGG 0.491901 8:-80174416 MsG0880046936.01.T01:CDS
ATTTGGTTATGACCATGTTA+AGG 0.496635 8:-80174780 MsG0880046936.01.T01:CDS
AGTCAAGCTCATAAAGTGTT+GGG 0.502165 8:+80174819 None:intergenic
GAAAGTCTTCTTCCTCTTGG+AGG 0.505548 8:-80174134 MsG0880046936.01.T01:CDS
TCTGGGTATTCAAATTCAAC+CGG 0.507511 8:+80174216 None:intergenic
CATTCTTCTTATGATGAGGA+TGG 0.510721 8:-80175100 MsG0880046936.01.T01:CDS
GTACACCTAATGTTGTGTTA+TGG 0.512237 8:-80174892 MsG0880046936.01.T01:CDS
AATATGGCAGAGCTTGTTGT+AGG 0.515355 8:+80175065 None:intergenic
TTGTCAGGTAGTTATTTACA+AGG 0.523979 8:-80174156 MsG0880046936.01.T01:CDS
ACCAAGCTCCTCAGTCATCT+GGG 0.524636 8:+80174199 None:intergenic
ATTGAACATCCTATTGGAAA+GGG 0.538458 8:-80174284 MsG0880046936.01.T01:CDS
TAGTCAAGCTCATAAAGTGT+TGG 0.539904 8:+80174818 None:intergenic
CATTGAACATCCTATTGGAA+AGG 0.539919 8:-80174285 MsG0880046936.01.T01:CDS
CCTATTGGAAAGGGAAGTAA+TGG 0.540984 8:-80174275 MsG0880046936.01.T01:CDS
CAACATTAGGTGTACGCCCT+TGG 0.541257 8:+80174900 None:intergenic
AGATCATGTTACGACTGAAA+AGG 0.549746 8:-80175281 MsG0880046936.01.T01:CDS
ACCCAGATGACTGAGGAGCT+TGG 0.552832 8:-80174200 MsG0880046936.01.T01:CDS
CCATTCTCTTGAAATGGAGG+TGG 0.553221 8:+80174974 None:intergenic
TTTGGGTGAACTCGGAGTGA+AGG 0.555736 8:-80174348 MsG0880046936.01.T01:CDS
CCATGTTAAGGATGACTACA+AGG 0.573407 8:-80174768 MsG0880046936.01.T01:CDS
ACTCAATAGCGCTATGAGGA+AGG 0.576120 8:+80174843 None:intergenic
TAATCATTCTTCTTATGATG+AGG 0.579629 8:-80175104 MsG0880046936.01.T01:CDS
TATTGAAGTTGGGATAGTAT+GGG 0.582348 8:-80174696 MsG0880046936.01.T01:CDS
ATAATTTGAGATCAAACCAA+TGG 0.586703 8:+80174400 None:intergenic
TGGCCATTCTCTTGAAATGG+AGG 0.591233 8:+80174971 None:intergenic
GGTGTCATGTCGTTACATAT+GGG 0.592164 8:-80174675 MsG0880046936.01.T01:CDS
ATGGCAGAGCTTGTTGTAGG+AGG 0.594532 8:+80175068 None:intergenic
CCTTGTAGTCATCCTTAACA+TGG 0.598335 8:+80174768 None:intergenic
GGGTACTCAATAGCGCTATG+AGG 0.603788 8:+80174839 None:intergenic
GGCATTGTCTGTCTCTACCA+AGG 0.613087 8:-80174917 MsG0880046936.01.T01:CDS
CAATCATTTATTCCTCCAAG+AGG 0.613135 8:+80174122 None:intergenic
GAATTATCATCCATATCAGA+AGG 0.613890 8:+80174467 None:intergenic
TGAGGAGCTTGGTGTTAAAG+GGG 0.617418 8:-80174189 MsG0880046936.01.T01:CDS
GCATTGTCTGTCTCTACCAA+GGG 0.623045 8:-80174916 MsG0880046936.01.T01:CDS
GGATGTTCAATGCCAAGTAA+GGG 0.623759 8:+80174296 None:intergenic
TACTAGATATGAAATAATTG+CGG 0.649269 8:+80175125 None:intergenic
ACACTAGTCCCATAACATTG+AGG 0.670032 8:+80174602 None:intergenic
CAGAAAGCAAGTATAACACA+TGG 0.673219 8:+80175032 None:intergenic
TTTGAATACCCAGATGACTG+AGG 0.686881 8:-80174207 MsG0880046936.01.T01:CDS
ATGAGGTGTTCTGTACTACA+CGG 0.766768 8:-80174493 MsG0880046936.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAATATAATTTTCAATATT+AGG + Chr8:80174706-80174725 None:intergenic 10.0%
!! ATTGAAAATTATATTGAAGT+TGG - Chr8:80174709-80174728 MsG0880046936.01.T01:CDS 15.0%
!! TTGAAAATTATATTGAAGTT+GGG - Chr8:80174710-80174729 MsG0880046936.01.T01:CDS 15.0%
!! ATAAAAAATTCAAGATCATC+AGG + Chr8:80174175-80174194 None:intergenic 20.0%
!! TACTAGATATGAAATAATTG+CGG + Chr8:80174294-80174313 None:intergenic 20.0%
!! TATATTTATCTTCATGGATT+TGG - Chr8:80174619-80174638 MsG0880046936.01.T01:CDS 20.0%
!!! ACTTTTCACATATCAATTTT+GGG - Chr8:80175051-80175070 MsG0880046936.01.T01:CDS 20.0%
!!! TACTTTTCACATATCAATTT+TGG - Chr8:80175050-80175069 MsG0880046936.01.T01:CDS 20.0%
!!! TGAAATTAGAGTTTTGTAAT+AGG + Chr8:80174261-80174280 None:intergenic 20.0%
! ACATTCAAATTGTCAATAGT+TGG + Chr8:80174553-80174572 None:intergenic 25.0%
! AGAATAAGGTCAAATTAGAT+TGG - Chr8:80174419-80174438 MsG0880046936.01.T01:CDS 25.0%
! CATTCAAATTGTCAATAGTT+GGG + Chr8:80174552-80174571 None:intergenic 25.0%
! CTTGACTATATTTATCTTCA+TGG - Chr8:80174613-80174632 MsG0880046936.01.T01:CDS 25.0%
! TAATCATTCTTCTTATGATG+AGG - Chr8:80174312-80174331 MsG0880046936.01.T01:CDS 25.0%
!! ATAATTTGAGATCAAACCAA+TGG + Chr8:80175019-80175038 None:intergenic 25.0%
AACCGTTTCAAAGATTTAAG+AGG + Chr8:80174211-80174230 None:intergenic 30.0%
AAGTATAACACATGGTGATT+TGG + Chr8:80174379-80174398 None:intergenic 30.0%
AAGTCAAATGACACCAATAA+TGG + Chr8:80174898-80174917 None:intergenic 30.0%
ACAGTCTAAAAAGTAACTCT+TGG - Chr8:80174770-80174789 MsG0880046936.01.T01:CDS 30.0%
ATATTGAAGTTGGGATAGTA+TGG - Chr8:80174719-80174738 MsG0880046936.01.T01:CDS 30.0%
ATCTGAATGGAGTTTGATAT+TGG - Chr8:80174851-80174870 MsG0880046936.01.T01:CDS 30.0%
ATGGTATTAAATCAGTCCAT+TGG - Chr8:80175000-80175019 MsG0880046936.01.T01:CDS 30.0%
ATTGAACATCCTATTGGAAA+GGG - Chr8:80175132-80175151 MsG0880046936.01.T01:CDS 30.0%
ATTTGGTTATGACCATGTTA+AGG - Chr8:80174636-80174655 MsG0880046936.01.T01:CDS 30.0%
GAAAAAATGATGAACTAGTC+CGG - Chr8:80175181-80175200 MsG0880046936.01.T01:CDS 30.0%
GAATTATCATCCATATCAGA+AGG + Chr8:80174952-80174971 None:intergenic 30.0%
GACCCAAAATAAAAACAGAT+TGG + Chr8:80174468-80174487 None:intergenic 30.0%
TAGATATGCCTCAATGTTAT+GGG - Chr8:80174806-80174825 MsG0880046936.01.T01:CDS 30.0%
TATTGAAGTTGGGATAGTAT+GGG - Chr8:80174720-80174739 MsG0880046936.01.T01:CDS 30.0%
TCTCAACACAATCACAAAAT+CGG + Chr8:80174974-80174993 None:intergenic 30.0%
TGATACATATGAGCCATTAT+TGG - Chr8:80174882-80174901 MsG0880046936.01.T01:CDS 30.0%
TTAGTTGCGTACAAAAATCA+TGG - Chr8:80174230-80174249 MsG0880046936.01.T01:CDS 30.0%
TTGTCAGGTAGTTATTTACA+AGG - Chr8:80175260-80175279 MsG0880046936.01.T01:CDS 30.0%
! ACATGGTGATTTGGAAAATA+TGG + Chr8:80174370-80174389 None:intergenic 30.0%
! TGCCTCTTAAATCTTTGAAA+CGG - Chr8:80174206-80174225 MsG0880046936.01.T01:CDS 30.0%
!! ATATCAATTTTGGGTGAACT+CGG - Chr8:80175060-80175079 MsG0880046936.01.T01:CDS 30.0%
!! ATTTTGGGTCCTGTTATTAA+CGG - Chr8:80174478-80174497 MsG0880046936.01.T01:CDS 30.0%
!! TGGTGAGAATTTTGAGAATA+AGG - Chr8:80174405-80174424 MsG0880046936.01.T01:CDS 30.0%
!!! GGCCAATCTGTTTTTATTTT+GGG - Chr8:80174463-80174482 MsG0880046936.01.T01:CDS 30.0%
!!! TGGCCAATCTGTTTTTATTT+TGG - Chr8:80174462-80174481 MsG0880046936.01.T01:CDS 30.0%
AACTCCATTCAGATACACAA+AGG + Chr8:80174845-80174864 None:intergenic 35.0%
AGATCATGTTACGACTGAAA+AGG - Chr8:80174135-80174154 MsG0880046936.01.T01:CDS 35.0%
CAATCATTTATTCCTCCAAG+AGG + Chr8:80175297-80175316 None:intergenic 35.0%
CAGAAAGCAAGTATAACACA+TGG + Chr8:80174387-80174406 None:intergenic 35.0%
CATGTGTTATACTTGCTTTC+TGG - Chr8:80174385-80174404 MsG0880046936.01.T01:CDS 35.0%
CATTCTTCTTATGATGAGGA+TGG - Chr8:80174316-80174335 MsG0880046936.01.T01:CDS 35.0%
CATTGAACATCCTATTGGAA+AGG - Chr8:80175131-80175150 MsG0880046936.01.T01:CDS 35.0%
CTAGATATGCCTCAATGTTA+TGG - Chr8:80174805-80174824 MsG0880046936.01.T01:CDS 35.0%
GATTGTGTTGAGATACTTGA+TGG - Chr8:80174981-80175000 MsG0880046936.01.T01:CDS 35.0%
GTACACCTAATGTTGTGTTA+TGG - Chr8:80174524-80174543 MsG0880046936.01.T01:intron 35.0%
TAGTCAAGCTCATAAAGTGT+TGG + Chr8:80174601-80174620 None:intergenic 35.0%
TCTGGGTATTCAAATTCAAC+CGG + Chr8:80175203-80175222 None:intergenic 35.0%
TGGACTAAACTCTTCATTCT+TGG - Chr8:80175096-80175115 MsG0880046936.01.T01:CDS 35.0%
TTCAGTCGTAACATGATCTA+TGG + Chr8:80174134-80174153 None:intergenic 35.0%
! AGTCAAGCTCATAAAGTGTT+GGG + Chr8:80174600-80174619 None:intergenic 35.0%
AAGGAAAGTCTTCTTCCTCT+TGG - Chr8:80175279-80175298 MsG0880046936.01.T01:CDS 40.0%
ACACTAGTCCCATAACATTG+AGG + Chr8:80174817-80174836 None:intergenic 40.0%
AGGATGTTCAATGCCAAGTA+AGG + Chr8:80175124-80175143 None:intergenic 40.0%
CAGACAATGCCGTTAATAAC+AGG + Chr8:80174490-80174509 None:intergenic 40.0%
CCATGTTAAGGATGACTACA+AGG - Chr8:80174648-80174667 MsG0880046936.01.T01:CDS 40.0%
CCATTACTTCCCTTTCCAAT+AGG + Chr8:80175144-80175163 None:intergenic 40.0%
CCTATTGGAAAGGGAAGTAA+TGG - Chr8:80175141-80175160 MsG0880046936.01.T01:CDS 40.0%
CCTTGTAGTCATCCTTAACA+TGG + Chr8:80174651-80174670 None:intergenic 40.0%
GGATGTTCAATGCCAAGTAA+GGG + Chr8:80175123-80175142 None:intergenic 40.0%
GGGTTCCATAACACAACATT+AGG + Chr8:80174532-80174551 None:intergenic 40.0%
TTCATTCTTGGACCCTTACT+TGG - Chr8:80175108-80175127 MsG0880046936.01.T01:CDS 40.0%
TTTGAATACCCAGATGACTG+AGG - Chr8:80175209-80175228 MsG0880046936.01.T01:CDS 40.0%
! ATGAGGTGTTCTGTACTACA+CGG - Chr8:80174923-80174942 MsG0880046936.01.T01:CDS 40.0%
! CAATGTTATGGGACTAGTGT+AGG - Chr8:80174817-80174836 MsG0880046936.01.T01:CDS 40.0%
! CTTGGCATTGAACATCCTAT+TGG - Chr8:80175126-80175145 MsG0880046936.01.T01:CDS 40.0%
! GATTGGCCATTCTCTTGAAA+TGG + Chr8:80174451-80174470 None:intergenic 40.0%
! GGTGTCATGTCGTTACATAT+GGG - Chr8:80174741-80174760 MsG0880046936.01.T01:CDS 40.0%
! GTCATTTGACTTATGCGATG+AGG - Chr8:80174906-80174925 MsG0880046936.01.T01:CDS 40.0%
!! AATATGGCAGAGCTTGTTGT+AGG + Chr8:80174354-80174373 None:intergenic 40.0%
AACTCGGAGTGAAGGAATCT+TGG - Chr8:80175076-80175095 MsG0880046936.01.T01:CDS 45.0%
ACTCAATAGCGCTATGAGGA+AGG + Chr8:80174576-80174595 None:intergenic 45.0%
CCACCTCCATTTCAAGAGAA+TGG - Chr8:80174442-80174461 MsG0880046936.01.T01:CDS 45.0%
CCATTCTCTTGAAATGGAGG+TGG + Chr8:80174445-80174464 None:intergenic 45.0%
GAAAGTCTTCTTCCTCTTGG+AGG - Chr8:80175282-80175301 MsG0880046936.01.T01:CDS 45.0%
GCATTGTCTGTCTCTACCAA+GGG - Chr8:80174500-80174519 MsG0880046936.01.T01:intron 45.0%
GGTGCCTTTGTGTATCTGAA+TGG - Chr8:80174838-80174857 MsG0880046936.01.T01:CDS 45.0%
TACACGGATGCCTTCTGATA+TGG - Chr8:80174939-80174958 MsG0880046936.01.T01:CDS 45.0%
TGGCCATTCTCTTGAAATGG+AGG + Chr8:80174448-80174467 None:intergenic 45.0%
! GGGTGTCATGTCGTTACATA+TGG - Chr8:80174740-80174759 MsG0880046936.01.T01:CDS 45.0%
!! ACTGAGGAGCTTGGTGTTAA+AGG - Chr8:80175225-80175244 MsG0880046936.01.T01:CDS 45.0%
!! CTGAGGAGCTTGGTGTTAAA+GGG - Chr8:80175226-80175245 MsG0880046936.01.T01:CDS 45.0%
!! TGAGGAGCTTGGTGTTAAAG+GGG - Chr8:80175227-80175246 MsG0880046936.01.T01:CDS 45.0%
ACCAAGCTCCTCAGTCATCT+GGG + Chr8:80175220-80175239 None:intergenic 50.0%
CAACATTAGGTGTACGCCCT+TGG + Chr8:80174519-80174538 None:intergenic 50.0%
GGCATTGTCTGTCTCTACCA+AGG - Chr8:80174499-80174518 MsG0880046936.01.T01:intron 50.0%
GGGTACTCAATAGCGCTATG+AGG + Chr8:80174580-80174599 None:intergenic 50.0%
TTTGGGTGAACTCGGAGTGA+AGG - Chr8:80175068-80175087 MsG0880046936.01.T01:CDS 50.0%
! AGGGGAAGCGTATTGTTGTC+AGG - Chr8:80175245-80175264 MsG0880046936.01.T01:CDS 50.0%
!! ATGGCAGAGCTTGTTGTAGG+AGG + Chr8:80174351-80174370 None:intergenic 50.0%
ACCCAGATGACTGAGGAGCT+TGG - Chr8:80175216-80175235 MsG0880046936.01.T01:CDS 55.0%
CACCAAGCTCCTCAGTCATC+TGG + Chr8:80175221-80175240 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 80174121 80175317 80174121 ID=MsG0880046936.01;Name=MsG0880046936.01
Chr8 mRNA 80174121 80175317 80174121 ID=MsG0880046936.01.T01;Parent=MsG0880046936.01;Name=MsG0880046936.01.T01;_AED=0.27;_eAED=0.27;_QI=0|0|0|1|1|1|2|0|367
Chr8 exon 80174604 80175317 80174604 ID=MsG0880046936.01.T01:exon:10273;Parent=MsG0880046936.01.T01
Chr8 exon 80174121 80174510 80174121 ID=MsG0880046936.01.T01:exon:10272;Parent=MsG0880046936.01.T01
Chr8 CDS 80174604 80175317 80174604 ID=MsG0880046936.01.T01:cds;Parent=MsG0880046936.01.T01
Chr8 CDS 80174121 80174510 80174121 ID=MsG0880046936.01.T01:cds;Parent=MsG0880046936.01.T01
Gene Sequence

>MsG0880046936.01.T01

ATGGAGAAATCCATAGATCATGTTACGACTGAAAAGGTTAGCAATCATATTCCTGATGATCTTGAATTTTTTATTGTATCTAAATTGCCTCTTAAATCTTTGAAACGGTTTAGTTGCGTACAAAAATCATGGTCACTCCTATTACAAAACTCTAATTTCATGAACATGTACCGCAATTATTTCATATCTAGTAATCATTCTTCTTATGATGAGGATGGTTCGTGTCTCCTCCTACAACAAGCTCTGCCATATTTTCCAAATCACCATGTGTTATACTTGCTTTCTGGTGAGAATTTTGAGAATAAGGTCAAATTAGATTGGCCACCTCCATTTCAAGAGAATGGCCAATCTGTTTTTATTTTGGGTCCTGTTATTAACGGCATTGTCTGTCTCTACCAAGGGCGTACACCTAATGTTGTGTTATGGAACCCAACTATTGACAATTTGAATGTCCTTCCTCATAGCGCTATTGAGTACCCAACACTTTATGAGCTTGACTATATTTATCTTCATGGATTTGGTTATGACCATGTTAAGGATGACTACAAGGTGATTCGATATGCATCTTATACTCTTGATGCACCTAATATTGAAAATTATATTGAAGTTGGGATAGTATGGGTGTCATGTCGTTACATATGGGAGATATACAGTCTAAAAAGTAACTCTTGGAGAAAAATTGATCTAGATATGCCTCAATGTTATGGGACTAGTGTGTTCTGTACTACACGGATGCCTTCTGATATGGATGATAATTCCGATTTTGTGATTGTGTTGAGATACTTGATGGTATTAAATCAGTCCATTGGTTTGATCTCAAATTATTTTGAGACAACTACTTTTCACATATCAATTTTGGGTGAACTCGGAGTGAAGGAATCTTGGACTAAACTCTTCATTCTTGGACCCTTACTTGGCATTGAACATCCTATTGGAAAGGGAAGTAATGGCGATTTATTCTTTAGAAGAAAAAATGATGAACTAGTCCGGTTGAATTTGAATACCCAGATGACTGAGGAGCTTGGTGTTAAAGGGGAAGCGTATTGTTGTCAGGTAGTTATTTACAAGGAAAGTCTTCTTCCTCTTGGAGGAATAAATGATTGA
Protein sequence

>MsG0880046936.01.T01

MEKSIDHVTTEKVSNHIPDDLEFFIVSKLPLKSLKRFSCVQKSWSLLLQNSNFMNMYRNYFISSNHSSYDEDGSCLLLQQALPYFPNHHVLYLLSGENFENKVKLDWPPPFQENGQSVFILGPVINGIVCLYQGRTPNVVLWNPTIDNLNVLPHSAIEYPTLYELDYIYLHGFGYDHVKDDYKVIRYASYTLDAPNIENYIEVGIVWVSCRYIWEIYSLKSNSWRKIDLDMPQCYGTSVFCTTRMPSDMDDNSDFVIVLRYLMVLNQSIGLISNYFETTTFHISILGELGVKESWTKLFILGPLLGIEHPIGKGSNGDLFFRRKNDELVRLNLNTQMTEELGVKGEAYCCQVVIYKESLLPLGGIND*