AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680034899.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034899.01.T01 MTR_6g078010 63.127 339 86 5 5 343 31 330 8.73e-146 416
MsG0680034899.01.T01 MTR_6g086825 63.609 338 100 7 9 345 142 457 1.79e-140 406
MsG0680034899.01.T01 MTR_6g079810 57.267 344 129 6 3 344 132 459 2.80e-128 375
MsG0680034899.01.T01 MTR_0134s0060 58.112 339 126 5 7 342 81 406 1.01e-126 369
MsG0680034899.01.T01 MTR_6g090250 54.108 353 146 7 4 346 76 422 4.21e-122 358
MsG0680034899.01.T01 MTR_6g079770 56.012 341 134 5 7 344 86 413 5.20e-119 350
MsG0680034899.01.T01 MTR_6g478230 56.609 348 99 8 9 345 98 404 1.10e-118 349
MsG0680034899.01.T01 MTR_0054s0100 53.959 341 136 4 6 345 85 405 6.40e-117 344
MsG0680034899.01.T01 MTR_0054s0090 53.666 341 135 4 6 345 85 403 2.66e-116 343
MsG0680034899.01.T01 MTR_4g028510 54.070 344 132 4 2 344 80 398 2.34e-115 342
MsG0680034899.01.T01 MTR_6g079790 55.077 325 123 6 22 344 2 305 4.13e-114 333
MsG0680034899.01.T01 MTR_4g130330 52.436 349 133 7 2 344 66 387 1.08e-107 320
MsG0680034899.01.T01 MTR_6g090180 52.174 345 144 6 3 346 112 436 1.94e-106 318
MsG0680034899.01.T01 MTR_4g028660 52.023 346 138 6 2 346 79 397 2.63e-106 317
MsG0680034899.01.T01 MTR_4g027450 52.023 346 138 6 2 346 79 397 2.63e-106 317
MsG0680034899.01.T01 MTR_6g080680 52.647 340 127 4 3 342 84 389 3.19e-106 317
MsG0680034899.01.T01 MTR_4g028680 50.145 345 143 6 2 344 79 396 2.15e-98 297
MsG0680034899.01.T01 MTR_6g087620 49.697 330 112 8 11 338 2 279 1.11e-95 286
MsG0680034899.01.T01 MTR_4g028750 48.624 327 137 8 2 326 79 376 1.52e-91 279
MsG0680034899.01.T01 MTR_7g023800 45.882 340 123 4 7 346 31 309 7.57e-89 269
MsG0680034899.01.T01 MTR_6g090260 63.500 200 61 2 149 346 73 262 8.18e-85 257
MsG0680034899.01.T01 MTR_0129s0070 46.334 341 149 9 6 344 84 392 9.12e-85 262
MsG0680034899.01.T01 MTR_8g063440 45.401 337 143 10 9 344 83 379 1.38e-82 256
MsG0680034899.01.T01 MTR_0129s0120 45.190 343 154 9 6 346 84 394 4.38e-82 255
MsG0680034899.01.T01 MTR_4g102410 43.109 341 160 11 10 345 136 447 2.45e-78 247
MsG0680034899.01.T01 MTR_5g097220 41.341 358 168 11 5 346 75 406 1.04e-76 241
MsG0680034899.01.T01 MTR_5g064700 41.880 351 173 12 7 344 32 364 6.93e-75 236
MsG0680034899.01.T01 MTR_2g021800 41.570 344 160 9 7 339 94 407 1.64e-74 236
MsG0680034899.01.T01 MTR_5g097680 41.408 355 169 11 9 344 89 423 4.34e-74 235
MsG0680034899.01.T01 MTR_0129s0050 40.922 347 167 7 5 339 79 399 1.06e-73 234
MsG0680034899.01.T01 MTR_2g021770 42.363 347 155 11 10 344 90 403 2.35e-72 230
MsG0680034899.01.T01 MTR_5g096630 39.943 348 169 10 10 345 92 411 3.52e-72 230
MsG0680034899.01.T01 MTR_2g022000 41.520 342 159 10 7 336 93 405 4.65e-72 230
MsG0680034899.01.T01 MTR_5g037680 41.499 347 138 11 10 342 85 380 7.06e-72 230
MsG0680034899.01.T01 MTR_2g021950 42.241 348 161 10 2 344 81 393 7.28e-71 226
MsG0680034899.01.T01 MTR_5g097330 41.311 351 168 10 3 346 82 401 2.37e-70 225
MsG0680034899.01.T01 MTR_6g461660 41.570 344 163 9 9 346 87 398 6.81e-70 224
MsG0680034899.01.T01 MTR_4g117550 41.716 338 139 9 6 342 87 367 2.94e-69 221
MsG0680034899.01.T01 MTR_5g097350 41.298 339 147 11 10 346 97 385 1.96e-68 219
MsG0680034899.01.T01 MTR_8g064890 40.351 342 154 8 10 344 86 384 2.90e-68 219
MsG0680034899.01.T01 MTR_2g104340 41.088 331 154 9 10 330 80 379 1.96e-67 217
MsG0680034899.01.T01 MTR_5g092850 42.059 340 172 11 10 346 83 400 2.97e-67 217
MsG0680034899.01.T01 MTR_7g017130 41.003 339 166 11 7 344 88 393 1.85e-66 215
MsG0680034899.01.T01 MTR_3g011120 39.577 331 149 11 11 337 86 369 5.80e-66 213
MsG0680034899.01.T01 MTR_2g038680 39.601 351 166 12 8 344 89 407 7.91e-65 211
MsG0680034899.01.T01 MTR_5g069390 38.218 348 162 10 10 343 104 412 1.83e-64 212
MsG0680034899.01.T01 MTR_5g096910 40.058 347 150 13 10 344 89 389 6.52e-64 208
MsG0680034899.01.T01 MTR_4g007940 36.550 342 159 9 10 334 28 328 7.18e-64 206
MsG0680034899.01.T01 MTR_8g012090 39.812 319 126 6 5 317 76 334 1.33e-63 205
MsG0680034899.01.T01 MTR_2g066200 39.429 350 159 13 6 345 79 385 2.75e-63 206
MsG0680034899.01.T01 MTR_7g017170 39.940 333 166 11 7 338 88 387 4.99e-63 206
MsG0680034899.01.T01 MTR_6g079730 66.207 145 49 0 202 346 4 148 5.81e-63 197
MsG0680034899.01.T01 MTR_8g464770 39.003 341 151 11 11 344 86 376 8.98e-63 204
MsG0680034899.01.T01 MTR_0831s0010 37.037 351 170 10 1 346 115 419 3.06e-62 204
MsG0680034899.01.T01 MTR_5g059430 37.677 353 165 9 10 345 109 423 4.33e-62 204
MsG0680034899.01.T01 MTR_7g069550 39.359 343 153 12 10 339 90 390 5.85e-62 203
MsG0680034899.01.T01 MTR_8g467670 39.881 336 146 13 10 339 85 370 1.31e-61 202
MsG0680034899.01.T01 MTR_5g074800 39.766 342 153 13 11 339 34 335 1.57e-61 200
MsG0680034899.01.T01 MTR_5g043605 39.583 336 154 12 11 343 45 334 5.56e-61 199
MsG0680034899.01.T01 MTR_1g007410 40.988 344 149 15 9 339 95 397 9.13e-61 200
MsG0680034899.01.T01 MTR_7g017040 39.823 339 176 10 9 346 85 396 1.88e-60 199
MsG0680034899.01.T01 MTR_8g041270 38.000 350 170 10 10 344 85 402 6.44e-60 198
MsG0680034899.01.T01 MTR_5g076490 40.290 345 155 12 9 344 30 332 8.81e-60 196
MsG0680034899.01.T01 MTR_4g055820 41.447 304 138 8 10 304 87 359 1.73e-59 196
MsG0680034899.01.T01 MTR_2g066170 37.609 343 169 11 6 343 79 381 1.89e-59 196
MsG0680034899.01.T01 MTR_5g074850 40.122 329 151 14 10 324 104 400 3.13e-59 205
MsG0680034899.01.T01 MTR_2g104280 36.103 349 177 10 11 344 84 401 7.50e-59 195
MsG0680034899.01.T01 MTR_7g111700 40.233 343 143 10 9 344 74 361 1.96e-58 193
MsG0680034899.01.T01 MTR_2g021920 45.783 249 114 6 103 344 35 269 2.09e-58 190
MsG0680034899.01.T01 MTR_1g007450 39.820 334 166 11 6 334 79 382 3.60e-58 194
MsG0680034899.01.T01 MTR_2g065860 37.243 341 170 9 10 344 82 384 4.37e-58 192
MsG0680034899.01.T01 MTR_7g104710 44.061 261 91 4 3 263 81 286 1.80e-57 189
MsG0680034899.01.T01 MTR_5g075370 38.554 332 163 13 10 328 77 380 3.28e-57 198
MsG0680034899.01.T01 MTR_5g092860 40.181 331 152 10 10 334 68 358 9.52e-57 189
MsG0680034899.01.T01 MTR_2g065840 36.390 349 163 10 10 344 82 385 3.86e-56 187
MsG0680034899.01.T01 MTR_4g014370 36.236 356 176 10 8 345 84 406 5.51e-56 188
MsG0680034899.01.T01 MTR_5g072060 40.491 326 125 13 11 323 78 347 3.42e-55 191
MsG0680034899.01.T01 MTR_2g034700 37.941 340 152 10 13 346 88 374 6.18e-55 184
MsG0680034899.01.T01 MTR_6g004450 43.902 246 107 8 104 345 48 266 7.60e-54 178
MsG0680034899.01.T01 MTR_5g068490 36.923 325 145 10 19 343 8 272 8.79e-54 178
MsG0680034899.01.T01 MTR_8g033140 35.475 358 169 12 10 342 92 412 1.91e-53 181
MsG0680034899.01.T01 MTR_1g007430 39.145 304 139 9 1 301 59 319 2.74e-53 178
MsG0680034899.01.T01 MTR_8g046310 35.294 340 155 11 13 343 90 373 9.71e-53 178
MsG0680034899.01.T01 MTR_5g022850 37.143 350 164 14 10 344 41 349 1.08e-52 177
MsG0680034899.01.T01 MTR_2g066150 35.882 340 154 10 10 345 78 357 3.77e-52 176
MsG0680034899.01.T01 MTR_5g097850 49.490 196 75 6 156 344 63 241 8.75e-52 172
MsG0680034899.01.T01 MTR_5g091140 38.415 328 145 10 10 330 122 399 8.84e-52 177
MsG0680034899.01.T01 MTR_8g033090 35.475 358 171 12 12 345 88 409 5.33e-51 175
MsG0680034899.01.T01 MTR_5g024260 37.318 343 139 15 11 327 93 385 9.22e-51 174
MsG0680034899.01.T01 MTR_3g018750 41.569 255 122 9 103 346 1 239 2.00e-50 168
MsG0680034899.01.T01 MTR_8g046200 33.158 380 159 12 13 344 86 418 2.55e-50 173
MsG0680034899.01.T01 MTR_1g053510 36.311 347 169 13 13 343 91 401 4.90e-50 172
MsG0680034899.01.T01 MTR_5g086590 39.847 261 117 5 60 320 11 231 3.78e-49 165
MsG0680034899.01.T01 MTR_8g039500 36.741 313 150 11 10 317 77 346 7.47e-48 165
MsG0680034899.01.T01 MTR_7g079640 35.890 326 141 12 9 324 85 352 1.77e-47 165
MsG0680034899.01.T01 MTR_5g023970 46.798 203 92 6 145 345 5 193 2.01e-47 159
MsG0680034899.01.T01 MTR_1g050312 36.585 328 133 12 10 329 16 276 3.08e-47 162
MsG0680034899.01.T01 MTR_8g018110 32.493 357 174 11 9 345 85 394 1.87e-46 162
MsG0680034899.01.T01 MTR_6g070880 47.059 170 81 4 4 171 97 259 4.29e-46 158
MsG0680034899.01.T01 MTR_6g071420 47.059 170 81 4 4 171 97 259 4.29e-46 158
MsG0680034899.01.T01 MTR_8g032980 33.062 369 182 11 8 342 78 415 4.36e-46 162
MsG0680034899.01.T01 MTR_8g033000 34.865 370 172 13 6 342 24 357 3.77e-45 158
MsG0680034899.01.T01 MTR_2g064950 35.328 351 165 13 5 339 77 381 5.95e-45 159
MsG0680034899.01.T01 MTR_8g033110 34.722 360 178 14 10 345 86 412 1.19e-44 158
MsG0680034899.01.T01 MTR_8g064790 32.840 338 140 9 8 344 82 333 8.01e-44 154
MsG0680034899.01.T01 MTR_8g063350 34.024 338 168 14 10 338 45 336 2.01e-43 153
MsG0680034899.01.T01 MTR_5g066420 47.771 157 69 3 182 335 1 147 2.56e-43 147
MsG0680034899.01.T01 MTR_8g046220 31.608 367 161 13 13 344 91 402 2.23e-42 152
MsG0680034899.01.T01 MTR_8g013000 38.538 301 138 12 13 303 87 350 6.48e-41 148
MsG0680034899.01.T01 MTR_1g098180 33.559 295 128 10 62 342 120 360 1.62e-40 146
MsG0680034899.01.T01 MTR_7g016930 36.786 280 137 9 2 277 59 302 1.74e-40 145
MsG0680034899.01.T01 MTR_6g009350 39.048 210 103 7 105 311 8 195 9.44e-40 139
MsG0680034899.01.T01 MTR_8g063330 31.126 302 164 6 10 306 89 351 1.15e-39 146
MsG0680034899.01.T01 MTR_4g016000 38.912 239 95 7 67 301 2 193 1.45e-39 139
MsG0680034899.01.T01 MTR_5g096920 46.341 164 71 4 182 344 1 148 2.89e-39 136
MsG0680034899.01.T01 MTR_8g063370 31.287 342 188 11 3 339 84 383 6.62e-39 142
MsG0680034899.01.T01 MTR_4g014370 42.211 199 93 5 151 345 154 334 3.62e-38 139
MsG0680034899.01.T01 MTR_7g019250 35.798 257 119 8 76 330 206 418 3.08e-37 139
MsG0680034899.01.T01 MTR_5g097660 54.310 116 52 1 232 346 3 118 3.13e-37 130
MsG0680034899.01.T01 MTR_8g036970 43.915 189 82 5 151 336 147 314 1.26e-36 136
MsG0680034899.01.T01 MTR_6g465360 49.333 150 69 4 7 153 131 276 4.25e-36 132
MsG0680034899.01.T01 MTR_3g030620 43.353 173 79 5 173 339 14 173 4.45e-34 124
MsG0680034899.01.T01 MTR_8g063310 31.195 343 180 14 10 339 89 388 5.14e-34 129
MsG0680034899.01.T01 MTR_8g035690 32.550 298 148 8 68 342 30 297 9.03e-34 127
MsG0680034899.01.T01 MTR_7g017150 42.132 197 98 5 151 345 82 264 5.27e-33 124
MsG0680034899.01.T01 MTR_7g017120 42.132 197 98 5 151 345 81 263 5.32e-33 124
MsG0680034899.01.T01 MTR_4g094400 52.586 116 35 2 178 282 6 112 1.78e-32 118
MsG0680034899.01.T01 MTR_5g096900 36.364 286 106 12 9 271 81 313 3.14e-32 123
MsG0680034899.01.T01 MTR_2g022020 45.763 118 62 1 227 344 1 116 5.18e-29 108
MsG0680034899.01.T01 MTR_5g036250 56.250 96 41 1 244 339 8 102 6.65e-29 110
MsG0680034899.01.T01 MTR_5g097540 37.374 198 95 7 107 303 61 230 3.61e-28 111
MsG0680034899.01.T01 MTR_5g098950 34.568 243 101 6 64 306 90 274 4.67e-28 110
MsG0680034899.01.T01 MTR_5g086780 44.697 132 72 1 194 324 97 228 1.82e-27 108
MsG0680034899.01.T01 MTR_5g094350 32.051 234 118 7 6 233 78 276 2.29e-27 108
MsG0680034899.01.T01 MTR_2g104260 33.636 220 110 9 11 222 84 275 4.63e-24 100
MsG0680034899.01.T01 MTR_7g101200 33.202 253 100 14 61 305 37 228 2.31e-22 94.4
MsG0680034899.01.T01 MTR_5g097820 35.079 191 83 8 12 192 98 257 9.87e-22 93.6
MsG0680034899.01.T01 MTR_2g031640 28.146 302 156 16 37 325 87 340 3.15e-21 94.0
MsG0680034899.01.T01 MTR_1g068835 25.153 326 190 13 3 320 55 334 7.55e-21 92.8
MsG0680034899.01.T01 MTR_1g050308 54.118 85 37 1 242 324 242 326 1.51e-20 91.7
MsG0680034899.01.T01 MTR_1g050308 32.353 170 78 6 10 174 105 242 5.71e-17 81.3
MsG0680034899.01.T01 MTR_4g133170 25.524 286 161 12 41 318 89 330 2.52e-20 91.3
MsG0680034899.01.T01 MTR_2g026010 26.936 297 169 13 37 323 85 343 2.97e-20 91.3
MsG0680034899.01.T01 MTR_1g068860 25.382 327 192 13 3 321 55 337 4.10e-20 90.5
MsG0680034899.01.T01 MTR_1g047080 27.434 339 154 13 9 343 28 278 6.99e-20 88.6
MsG0680034899.01.T01 MTR_5g096050 44.954 109 59 1 182 290 1 108 7.74e-20 84.3
MsG0680034899.01.T01 MTR_2g028140 26.061 330 186 18 3 320 81 364 3.80e-19 88.2
MsG0680034899.01.T01 MTR_1g068875 26.364 330 182 19 3 321 55 334 5.11e-19 87.4
MsG0680034899.01.T01 MTR_2g031720 25.478 314 168 16 26 321 78 343 7.89e-19 87.0
MsG0680034899.01.T01 MTR_1g076810 35.556 180 84 7 9 183 89 241 1.86e-18 87.0
MsG0680034899.01.T01 MTR_5g091150 33.032 221 70 9 37 254 39 184 2.08e-18 82.8
MsG0680034899.01.T01 MTR_2g028130 24.786 351 190 16 3 320 56 365 3.65e-18 85.5
MsG0680034899.01.T01 MTR_2g426070 27.687 307 167 18 29 321 92 357 4.67e-18 85.1
MsG0680034899.01.T01 MTR_2g426090 25.545 321 179 16 21 321 74 354 8.92e-18 84.3
MsG0680034899.01.T01 MTR_2g024100 24.922 321 176 12 26 321 81 361 2.89e-17 82.8
MsG0680034899.01.T01 MTR_1g050305 50.000 88 38 3 249 331 21 107 1.15e-16 76.3
MsG0680034899.01.T01 MTR_1g115205 33.333 183 87 7 12 190 86 237 1.16e-16 79.0
MsG0680034899.01.T01 MTR_3g083010 22.507 351 203 15 4 321 58 372 3.32e-16 79.7
MsG0680034899.01.T01 MTR_2g426060 24.490 294 172 15 41 320 98 355 5.49e-16 79.0
MsG0680034899.01.T01 MTR_3g097190 53.125 64 30 0 268 331 28 91 8.43e-16 75.1
MsG0680034899.01.T01 MTR_4g070550 25.085 295 173 13 42 322 101 361 1.16e-15 77.8
MsG0680034899.01.T01 MTR_2g031680 27.304 293 153 14 41 320 90 335 1.73e-14 74.3
MsG0680034899.01.T01 MTR_2g028110 25.246 305 177 16 26 321 75 337 4.35e-14 73.2
MsG0680034899.01.T01 MTR_7g017270 55.556 63 15 1 232 294 38 87 2.73e-13 65.5
MsG0680034899.01.T01 MTR_5g037190 29.630 189 92 6 7 194 86 234 3.91e-13 68.9
MsG0680034899.01.T01 MTR_2g021050 24.852 338 172 17 3 321 56 330 4.30e-13 70.1
MsG0680034899.01.T01 MTR_8g099460 24.765 319 186 16 16 320 67 345 6.81e-13 69.7
MsG0680034899.01.T01 MTR_6g065980 26.644 289 141 15 43 329 111 330 5.15e-12 66.6
MsG0680034899.01.T01 MTR_4g061340 28.049 246 132 9 42 287 119 319 8.66e-12 66.2
MsG0680034899.01.T01 MTR_0044s0300 24.490 294 157 14 43 334 138 368 2.48e-11 64.7
MsG0680034899.01.T01 MTR_3g036335 26.515 264 133 10 23 284 123 327 2.79e-11 64.7
MsG0680034899.01.T01 MTR_3g024130 25.714 245 131 8 42 286 60 253 4.38e-11 63.5
MsG0680034899.01.T01 MTR_8g102520 26.207 290 159 13 65 346 75 317 4.59e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034899.01.T01 AT4G12560 26.032 315 180 12 25 326 78 352 7.08e-19 87.4
MsG0680034899.01.T01 AT4G12560 26.032 315 180 12 25 326 78 352 7.08e-19 87.4
MsG0680034899.01.T01 AT4G12560 26.032 315 180 12 25 326 78 352 7.08e-19 87.4
MsG0680034899.01.T01 AT4G22390 22.500 320 188 14 25 329 78 352 6.84e-11 63.5
MsG0680034899.01.T01 AT4G22390 22.500 320 188 14 25 329 78 352 6.86e-11 63.5

Find 78 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTTGATTGTTTGGGTAAC+TGG 0.148754 6:-96403185 None:intergenic
AGAAACTTGGCAGTGTTAAA+TGG 0.237551 6:+96403707 MsG0680034899.01.T01:CDS
AATAATTATGGATATGAATA+TGG 0.251218 6:+96403557 MsG0680034899.01.T01:CDS
ATGACCCTTCTTCATGGATT+TGG 0.257099 6:+96403284 MsG0680034899.01.T01:CDS
TGGTTGTGTCAAAATAATTA+TGG 0.263338 6:+96403545 MsG0680034899.01.T01:CDS
GAGGAAGACACTTGATTGTT+TGG 0.291661 6:-96403193 None:intergenic
AACCAAGCTGCTCGATCATC+TGG 0.295825 6:-96403934 None:intergenic
CATTCTTCCAATTGAGGAAT+AGG 0.302947 6:+96404003 MsG0680034899.01.T01:CDS
CCTTGTGTTGAGTGTCCTAT+TGG 0.303711 6:+96403842 MsG0680034899.01.T01:CDS
TATCCCATTTCAACCTAGTT+TGG 0.313670 6:+96403598 MsG0680034899.01.T01:CDS
ATGGATATGAATATGGATAT+TGG 0.318905 6:+96403564 MsG0680034899.01.T01:CDS
GTTACCAAACTAGGTTGAAA+TGG 0.334259 6:-96403602 None:intergenic
TGGACCAATCTCTTCACTAT+TGG 0.339983 6:+96403812 MsG0680034899.01.T01:CDS
TCAATACGATCCGAAGAATA+AGG 0.348362 6:-96403488 None:intergenic
TATGTTATGACCCTTCTTCA+TGG 0.349073 6:+96403278 MsG0680034899.01.T01:CDS
TTAACTACCTATTCCTCAAT+TGG 0.356598 6:-96404010 None:intergenic
TGTTGACATGCCTTATTCTT+CGG 0.373233 6:+96403478 MsG0680034899.01.T01:CDS
AGGAAGACACTTGATTGTTT+GGG 0.389874 6:-96403192 None:intergenic
GAAGTTATGATAGAATTATC+AGG 0.391497 6:+96403350 MsG0680034899.01.T01:CDS
CTAATATCCGTATGGTCAGC+TGG 0.395342 6:-96403248 None:intergenic
ATCATCTACATCTGAGGGTA+TGG 0.398755 6:-96403660 None:intergenic
AATTTAGATGTTGAAATACT+TGG 0.402569 6:+96403080 MsG0680034899.01.T01:CDS
GATGTTGAAATACTTGGTTT+TGG 0.406609 6:+96403086 MsG0680034899.01.T01:CDS
GGAAATCATTCTTCCAATTG+AGG 0.437981 6:+96403997 MsG0680034899.01.T01:CDS
GAAGAATAAGGCATGTCAAC+AGG 0.451165 6:-96403476 None:intergenic
ATATCAATTATGGGTGAGAT+TGG 0.452163 6:+96403776 MsG0680034899.01.T01:CDS
GAAACTTGGCAGTGTTAAAT+GGG 0.461564 6:+96403708 MsG0680034899.01.T01:CDS
CCCTTCGTACCCACTCCAAT+AGG 0.463699 6:-96403857 None:intergenic
GAACAAACATTGGGCCATCA+TGG 0.463725 6:+96403414 MsG0680034899.01.T01:CDS
CCTAGTGAGATTGAGTTGGC+TGG 0.474040 6:+96403215 MsG0680034899.01.T01:CDS
TATTCTTCGGATCGTATTGA+GGG 0.475478 6:+96403491 MsG0680034899.01.T01:CDS
AAGTAAAACGTTACCAAACT+AGG 0.478192 6:-96403611 None:intergenic
TTATTCTTCGGATCGTATTG+AGG 0.487311 6:+96403490 MsG0680034899.01.T01:CDS
CATATCCAAATCCATGAAGA+AGG 0.488423 6:-96403289 None:intergenic
TTACCAAACTAGGTTGAAAT+GGG 0.489308 6:-96403601 None:intergenic
TGAAACAAATTGGAGAAACT+TGG 0.511083 6:+96403694 MsG0680034899.01.T01:CDS
TAAATGACTATAAAGTCATT+CGG 0.514322 6:+96403321 MsG0680034899.01.T01:CDS
ACTTGATTGTTTGGGTAACT+GGG 0.515046 6:-96403184 None:intergenic
CTCTCCACGAGTCACTTCTT+AGG 0.515400 6:-96403448 None:intergenic
ACATGGATGGAGTGTATCAT+TGG 0.515881 6:+96403525 MsG0680034899.01.T01:CDS
TCCTCCTAGTGAGATTGAGT+TGG 0.518662 6:+96403211 MsG0680034899.01.T01:CDS
TTGTAGGATAATAAATTACA+AGG 0.518948 6:+96403976 MsG0680034899.01.T01:CDS
AACAAACATTGGGCCATCAT+GGG 0.528352 6:+96403415 MsG0680034899.01.T01:CDS
CCTATTGGAGTGGGTACGAA+GGG 0.532436 6:+96403857 MsG0680034899.01.T01:CDS
TGAGGGCACCCAAGTCTACA+TGG 0.532671 6:+96403508 MsG0680034899.01.T01:CDS
GTCTATCCCAGCTGACCATA+CGG 0.533807 6:+96403241 MsG0680034899.01.T01:CDS
AGATTGGTGTGAAGGAATCA+TGG 0.533826 6:+96403792 MsG0680034899.01.T01:CDS
ACACTCCATCCATGTAGACT+TGG 0.534797 6:-96403517 None:intergenic
CTTTATAGTCATTTATAGCA+TGG 0.536983 6:-96403313 None:intergenic
CCAGCCAACTCAATCTCACT+AGG 0.544126 6:-96403215 None:intergenic
AAAAGAATCATCTACATCTG+AGG 0.560143 6:-96403666 None:intergenic
TAATATCCGTATGGTCAGCT+GGG 0.562587 6:-96403247 None:intergenic
TCCTATTGGAGTGGGTACGA+AGG 0.563560 6:+96403856 MsG0680034899.01.T01:CDS
AAAGAATCATCTACATCTGA+GGG 0.571534 6:-96403665 None:intergenic
GGCACCCAAGTCTACATGGA+TGG 0.576024 6:+96403512 MsG0680034899.01.T01:CDS
TCATCTACATCTGAGGGTAT+GGG 0.580225 6:-96403659 None:intergenic
TGGTCCAATAGTGAAGAGAT+TGG 0.582752 6:-96403816 None:intergenic
CTCGAAGATTATATATCTGT+AGG 0.583135 6:+96403383 MsG0680034899.01.T01:CDS
CACTCCATCCATGTAGACTT+GGG 0.584074 6:-96403516 None:intergenic
GAAATGATGAGGAACTAGCT+TGG 0.584973 6:+96403897 MsG0680034899.01.T01:CDS
TATGGGTGAGATTGGTGTGA+AGG 0.588992 6:+96403784 MsG0680034899.01.T01:CDS
GTTGAGTGTCCTATTGGAGT+GGG 0.589788 6:+96403848 MsG0680034899.01.T01:CDS
ACCAAGCTGCTCGATCATCT+GGG 0.590904 6:-96403933 None:intergenic
TGTTGAGTGTCCTATTGGAG+TGG 0.591016 6:+96403847 MsG0680034899.01.T01:CDS
ACCCAGATGATCGAGCAGCT+TGG 0.595795 6:+96403932 MsG0680034899.01.T01:CDS
AGGCTATATATCTCCCATGA+TGG 0.599780 6:-96403428 None:intergenic
AAATACTACTAATATCCGTA+TGG 0.603487 6:-96403256 None:intergenic
ATTCTTCATAAGAAATGATG+AGG 0.609058 6:+96403886 MsG0680034899.01.T01:CDS
ATAATATGAGATCAATGCAA+TGG 0.614224 6:-96403732 None:intergenic
CCAATAGGACACTCAACACA+AGG 0.623385 6:-96403842 None:intergenic
TATTGGAGTGGGTACGAAGG+GGG 0.628720 6:+96403859 MsG0680034899.01.T01:CDS
ATATCCAAATCCATGAAGAA+GGG 0.629021 6:-96403288 None:intergenic
TGTTATCGTAATAATGACGA+TGG 0.631174 6:-96403133 None:intergenic
GCCAACTCAATCTCACTAGG+AGG 0.647616 6:-96403212 None:intergenic
CTATTGGAGTGGGTACGAAG+GGG 0.648066 6:+96403858 MsG0680034899.01.T01:CDS
ATAGCCTAAGAAGTGACTCG+TGG 0.654513 6:+96403444 MsG0680034899.01.T01:CDS
ATAATAAACAAATTGCATTG+TGG 0.660444 6:+96403159 MsG0680034899.01.T01:CDS
GGACACTCAACACAAGGCAA+TGG 0.666920 6:-96403836 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATTATGGATATGAATA+TGG + Chr6:96403557-96403576 MsG0680034899.01.T01:CDS 15.0%
!!! ACATCTAAATTTTCTTTAAA+TGG - Chr6:96403071-96403090 None:intergenic 15.0%
!! AATTTAGATGTTGAAATACT+TGG + Chr6:96403080-96403099 MsG0680034899.01.T01:CDS 20.0%
!! ATAATAAACAAATTGCATTG+TGG + Chr6:96403159-96403178 MsG0680034899.01.T01:CDS 20.0%
!! TAAATGACTATAAAGTCATT+CGG + Chr6:96403321-96403340 MsG0680034899.01.T01:CDS 20.0%
!! TTGTAGGATAATAAATTACA+AGG + Chr6:96403976-96403995 MsG0680034899.01.T01:CDS 20.0%
!!! ACTTTTCACATATCAATTAT+GGG + Chr6:96403767-96403786 MsG0680034899.01.T01:CDS 20.0%
!!! TACTTTTCACATATCAATTA+TGG + Chr6:96403766-96403785 MsG0680034899.01.T01:CDS 20.0%
! AAATACTACTAATATCCGTA+TGG - Chr6:96403259-96403278 None:intergenic 25.0%
! ATAATATGAGATCAATGCAA+TGG - Chr6:96403735-96403754 None:intergenic 25.0%
! ATGGATATGAATATGGATAT+TGG + Chr6:96403564-96403583 MsG0680034899.01.T01:CDS 25.0%
! ATTCTTCATAAGAAATGATG+AGG + Chr6:96403886-96403905 MsG0680034899.01.T01:CDS 25.0%
! CTTTATAGTCATTTATAGCA+TGG - Chr6:96403316-96403335 None:intergenic 25.0%
! GAAGTTATGATAGAATTATC+AGG + Chr6:96403350-96403369 MsG0680034899.01.T01:CDS 25.0%
! TGGTTGTGTCAAAATAATTA+TGG + Chr6:96403545-96403564 MsG0680034899.01.T01:CDS 25.0%
!! CTTTTGATGTTGAAACAAAT+TGG + Chr6:96403684-96403703 MsG0680034899.01.T01:CDS 25.0%
!! GATGTTTTCTATTCTTTTAC+TGG + Chr6:96403005-96403024 MsG0680034899.01.T01:CDS 25.0%
!! TTATATATCTGTAGGCTTTT+CGG + Chr6:96403391-96403410 MsG0680034899.01.T01:CDS 25.0%
AAAAGAATCATCTACATCTG+AGG - Chr6:96403669-96403688 None:intergenic 30.0%
AAAGAATCATCTACATCTGA+GGG - Chr6:96403668-96403687 None:intergenic 30.0%
AAGTAAAACGTTACCAAACT+AGG - Chr6:96403614-96403633 None:intergenic 30.0%
ATATCAATTATGGGTGAGAT+TGG + Chr6:96403776-96403795 MsG0680034899.01.T01:CDS 30.0%
ATATCCAAATCCATGAAGAA+GGG - Chr6:96403291-96403310 None:intergenic 30.0%
CTCGAAGATTATATATCTGT+AGG + Chr6:96403383-96403402 MsG0680034899.01.T01:CDS 30.0%
TGAAACAAATTGGAGAAACT+TGG + Chr6:96403694-96403713 MsG0680034899.01.T01:CDS 30.0%
TGTTATCGTAATAATGACGA+TGG - Chr6:96403136-96403155 None:intergenic 30.0%
! GATGTTGAAATACTTGGTTT+TGG + Chr6:96403086-96403105 MsG0680034899.01.T01:CDS 30.0%
! TATATATCTGTAGGCTTTTC+GGG + Chr6:96403392-96403411 MsG0680034899.01.T01:CDS 30.0%
! TTACCAAACTAGGTTGAAAT+GGG - Chr6:96403604-96403623 None:intergenic 30.0%
ACTTGATTGTTTGGGTAACT+GGG - Chr6:96403187-96403206 None:intergenic 35.0%
AGAAACTTGGCAGTGTTAAA+TGG + Chr6:96403707-96403726 MsG0680034899.01.T01:CDS 35.0%
AGGAAGACACTTGATTGTTT+GGG - Chr6:96403195-96403214 None:intergenic 35.0%
CATATCCAAATCCATGAAGA+AGG - Chr6:96403292-96403311 None:intergenic 35.0%
GAAACTTGGCAGTGTTAAAT+GGG + Chr6:96403708-96403727 MsG0680034899.01.T01:CDS 35.0%
GGAAATCATTCTTCCAATTG+AGG + Chr6:96403997-96404016 MsG0680034899.01.T01:CDS 35.0%
TATCCCATTTCAACCTAGTT+TGG + Chr6:96403598-96403617 MsG0680034899.01.T01:CDS 35.0%
TATGTTATGACCCTTCTTCA+TGG + Chr6:96403278-96403297 MsG0680034899.01.T01:CDS 35.0%
TATTCTTCGGATCGTATTGA+GGG + Chr6:96403491-96403510 MsG0680034899.01.T01:CDS 35.0%
TCAATACGATCCGAAGAATA+AGG - Chr6:96403491-96403510 None:intergenic 35.0%
TTATTCTTCGGATCGTATTG+AGG + Chr6:96403490-96403509 MsG0680034899.01.T01:CDS 35.0%
! GTTACCAAACTAGGTTGAAA+TGG - Chr6:96403605-96403624 None:intergenic 35.0%
! TCTATTCTTTTACTGGTGAG+AGG + Chr6:96403012-96403031 MsG0680034899.01.T01:CDS 35.0%
! TGTTGACATGCCTTATTCTT+CGG + Chr6:96403478-96403497 MsG0680034899.01.T01:CDS 35.0%
!!! CTTGGTTTTGGTAGTATTAG+TGG + Chr6:96403098-96403117 MsG0680034899.01.T01:CDS 35.0%
AACAAACATTGGGCCATCAT+GGG + Chr6:96403415-96403434 MsG0680034899.01.T01:CDS 40.0%
ACATGGATGGAGTGTATCAT+TGG + Chr6:96403525-96403544 MsG0680034899.01.T01:CDS 40.0%
AGATTGGTGTGAAGGAATCA+TGG + Chr6:96403792-96403811 MsG0680034899.01.T01:CDS 40.0%
AGGCTATATATCTCCCATGA+TGG - Chr6:96403431-96403450 None:intergenic 40.0%
ATCATCTACATCTGAGGGTA+TGG - Chr6:96403663-96403682 None:intergenic 40.0%
ATGACCCTTCTTCATGGATT+TGG + Chr6:96403284-96403303 MsG0680034899.01.T01:CDS 40.0%
CACTTGATTGTTTGGGTAAC+TGG - Chr6:96403188-96403207 None:intergenic 40.0%
GAAATGATGAGGAACTAGCT+TGG + Chr6:96403897-96403916 MsG0680034899.01.T01:CDS 40.0%
GAAGAATAAGGCATGTCAAC+AGG - Chr6:96403479-96403498 None:intergenic 40.0%
GAGGAAGACACTTGATTGTT+TGG - Chr6:96403196-96403215 None:intergenic 40.0%
TAATATCCGTATGGTCAGCT+GGG - Chr6:96403250-96403269 None:intergenic 40.0%
TGGACCAATCTCTTCACTAT+TGG + Chr6:96403812-96403831 MsG0680034899.01.T01:CDS 40.0%
TGGTCCAATAGTGAAGAGAT+TGG - Chr6:96403819-96403838 None:intergenic 40.0%
! AAGCACAGAGTTTTCGTTGT+AGG + Chr6:96403960-96403979 MsG0680034899.01.T01:CDS 40.0%
! TCATCTACATCTGAGGGTAT+GGG - Chr6:96403662-96403681 None:intergenic 40.0%
!! GCTTTTCGGGAACAAACATT+GGG + Chr6:96403405-96403424 MsG0680034899.01.T01:CDS 40.0%
ACACTCCATCCATGTAGACT+TGG - Chr6:96403520-96403539 None:intergenic 45.0%
ATAGCCTAAGAAGTGACTCG+TGG + Chr6:96403444-96403463 MsG0680034899.01.T01:CDS 45.0%
CACTCCATCCATGTAGACTT+GGG - Chr6:96403519-96403538 None:intergenic 45.0%
CCAATAGGACACTCAACACA+AGG - Chr6:96403845-96403864 None:intergenic 45.0%
CCTTGTGTTGAGTGTCCTAT+TGG + Chr6:96403842-96403861 MsG0680034899.01.T01:CDS 45.0%
CTAATATCCGTATGGTCAGC+TGG - Chr6:96403251-96403270 None:intergenic 45.0%
GAACAAACATTGGGCCATCA+TGG + Chr6:96403414-96403433 MsG0680034899.01.T01:CDS 45.0%
GTTGAGTGTCCTATTGGAGT+GGG + Chr6:96403848-96403867 MsG0680034899.01.T01:CDS 45.0%
TATGGGTGAGATTGGTGTGA+AGG + Chr6:96403784-96403803 MsG0680034899.01.T01:CDS 45.0%
TCCTCCTAGTGAGATTGAGT+TGG + Chr6:96403211-96403230 MsG0680034899.01.T01:CDS 45.0%
TGTTGAGTGTCCTATTGGAG+TGG + Chr6:96403847-96403866 MsG0680034899.01.T01:CDS 45.0%
!! GGCTTTTCGGGAACAAACAT+TGG + Chr6:96403404-96403423 MsG0680034899.01.T01:CDS 45.0%
AACCAAGCTGCTCGATCATC+TGG - Chr6:96403937-96403956 None:intergenic 50.0%
ACCAAGCTGCTCGATCATCT+GGG - Chr6:96403936-96403955 None:intergenic 50.0%
CCAGCCAACTCAATCTCACT+AGG - Chr6:96403218-96403237 None:intergenic 50.0%
CCTATTGGAGTGGGTACGAA+GGG + Chr6:96403857-96403876 MsG0680034899.01.T01:CDS 50.0%
CTATTGGAGTGGGTACGAAG+GGG + Chr6:96403858-96403877 MsG0680034899.01.T01:CDS 50.0%
CTCTCCACGAGTCACTTCTT+AGG - Chr6:96403451-96403470 None:intergenic 50.0%
GCCAACTCAATCTCACTAGG+AGG - Chr6:96403215-96403234 None:intergenic 50.0%
GGACACTCAACACAAGGCAA+TGG - Chr6:96403839-96403858 None:intergenic 50.0%
GTCTATCCCAGCTGACCATA+CGG + Chr6:96403241-96403260 MsG0680034899.01.T01:CDS 50.0%
TATTGGAGTGGGTACGAAGG+GGG + Chr6:96403859-96403878 MsG0680034899.01.T01:CDS 50.0%
TCCTATTGGAGTGGGTACGA+AGG + Chr6:96403856-96403875 MsG0680034899.01.T01:CDS 50.0%
!! CCTAGTGAGATTGAGTTGGC+TGG + Chr6:96403215-96403234 MsG0680034899.01.T01:CDS 50.0%
ACCCAGATGATCGAGCAGCT+TGG + Chr6:96403932-96403951 MsG0680034899.01.T01:CDS 55.0%
CCCTTCGTACCCACTCCAAT+AGG - Chr6:96403860-96403879 None:intergenic 55.0%
GGCACCCAAGTCTACATGGA+TGG + Chr6:96403512-96403531 MsG0680034899.01.T01:CDS 55.0%
!! TGAGGGCACCCAAGTCTACA+TGG + Chr6:96403508-96403527 MsG0680034899.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 96402984 96404024 96402984 ID=MsG0680034899.01;Name=MsG0680034899.01
Chr6 mRNA 96402984 96404024 96402984 ID=MsG0680034899.01.T01;Parent=MsG0680034899.01;Name=MsG0680034899.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|346
Chr6 exon 96402984 96404024 96402984 ID=MsG0680034899.01.T01:exon:6843;Parent=MsG0680034899.01.T01
Chr6 CDS 96402984 96404024 96402984 ID=MsG0680034899.01.T01:cds;Parent=MsG0680034899.01.T01
Gene Sequence

>MsG0680034899.01.T01

ATGGATTTCAAACACAACAAAGATGTTTTCTATTCTTTTACTGGTGAGAGGTTTGAGAATAAAGTCAAATTAGATTTATCAAATCCATTTAAAGAAAATTTAGATGTTGAAATACTTGGTTTTGGTAGTATTAGTGGCATACTTTGTCTCCATCGTCATTATTACGATAACAGTGATAATAAACAAATTGCATTGTGGAACCCAGTTACCCAAACAATCAAGTGTCTTCCTCCTAGTGAGATTGAGTTGGCTGGATTGTCTATCCCAGCTGACCATACGGATATTAGTAGTATTTATGTTATGACCCTTCTTCATGGATTTGGATATGACCATGCTATAAATGACTATAAAGTCATTCGGTATATAGAAGTTATGATAGAATTATCAGGTAGATGTGAGCTCGAAGATTATATATCTGTAGGCTTTTCGGGAACAAACATTGGGCCATCATGGGAGATATATAGCCTAAGAAGTGACTCGTGGAGAGAGATTCCTGTTGACATGCCTTATTCTTCGGATCGTATTGAGGGCACCCAAGTCTACATGGATGGAGTGTATCATTGGTTGTGTCAAAATAATTATGGATATGAATATGGATATTGGAAAAAACATAATATCCCATTTCAACCTAGTTTGGTAACGTTTTACTTGAGCAACGAAGTGTTCTGCATAACACCCATACCCTCAGATGTAGATGATTCTTTTGATGTTGAAACAAATTGGAGAAACTTGGCAGTGTTAAATGGGACCATTGCATTGATCTCATATTATGAAAAGACGACTACTTTTCACATATCAATTATGGGTGAGATTGGTGTGAAGGAATCATGGACCAATCTCTTCACTATTGGACCATTGCCTTGTGTTGAGTGTCCTATTGGAGTGGGTACGAAGGGGGAAATATTCTTCATAAGAAATGATGAGGAACTAGCTTGGTTTGATTTGAGTACCCAGATGATCGAGCAGCTTGGTTATAAAGCACAGAGTTTTCGTTGTAGGATAATAAATTACAAGGAAATCATTCTTCCAATTGAGGAATAG
Protein sequence

>MsG0680034899.01.T01

MDFKHNKDVFYSFTGERFENKVKLDLSNPFKENLDVEILGFGSISGILCLHRHYYDNSDNKQIALWNPVTQTIKCLPPSEIELAGLSIPADHTDISSIYVMTLLHGFGYDHAINDYKVIRYIEVMIELSGRCELEDYISVGFSGTNIGPSWEIYSLRSDSWREIPVDMPYSSDRIEGTQVYMDGVYHWLCQNNYGYEYGYWKKHNIPFQPSLVTFYLSNEVFCITPIPSDVDDSFDVETNWRNLAVLNGTIALISYYEKTTTFHISIMGEIGVKESWTNLFTIGPLPCVECPIGVGTKGEIFFIRNDEELAWFDLSTQMIEQLGYKAQSFRCRIINYKEIILPIEE*