AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001512.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001512.01.T01 MTR_5g086590 74.449 227 57 1 245 470 5 231 4.86e-120 355
MsG0180001512.01.T01 MTR_5g037680 44.902 461 184 13 78 522 4 410 3.70e-110 337
MsG0180001512.01.T01 MTR_5g096910 46.028 428 167 10 69 486 4 377 3.04e-107 328
MsG0180001512.01.T01 MTR_0129s0070 43.488 430 174 10 67 480 2 378 9.07e-103 317
MsG0180001512.01.T01 MTR_5g069390 44.573 433 179 11 75 488 19 409 4.67e-101 315
MsG0180001512.01.T01 MTR_2g022000 42.035 452 189 7 67 489 1 408 3.91e-100 311
MsG0180001512.01.T01 MTR_0129s0120 43.619 431 172 11 67 480 2 378 8.83e-100 308
MsG0180001512.01.T01 MTR_5g059430 42.391 460 200 13 65 501 8 425 1.79e-97 303
MsG0180001512.01.T01 MTR_8g012090 43.165 417 140 10 65 467 1 334 1.30e-92 288
MsG0180001512.01.T01 MTR_2g021800 40.265 452 196 7 67 488 1 408 1.49e-92 290
MsG0180001512.01.T01 MTR_2g038680 42.291 454 164 14 70 487 5 396 2.74e-92 290
MsG0180001512.01.T01 MTR_3g011120 43.659 410 159 12 76 474 8 356 2.04e-90 283
MsG0180001512.01.T01 MTR_7g017170 38.863 422 195 7 65 473 1 372 1.08e-89 283
MsG0180001512.01.T01 MTR_5g097330 41.966 417 184 12 79 481 13 385 1.09e-89 283
MsG0180001512.01.T01 MTR_7g017130 38.863 422 195 7 65 473 1 372 1.18e-89 282
MsG0180001512.01.T01 MTR_6g086825 40.959 459 183 15 69 498 57 456 2.76e-88 281
MsG0180001512.01.T01 MTR_8g063440 43.521 409 160 13 79 474 9 359 8.78e-88 277
MsG0180001512.01.T01 MTR_5g096900 49.858 353 126 14 75 421 6 313 6.40e-87 273
MsG0180001512.01.T01 MTR_7g069550 40.000 420 172 12 79 473 11 375 2.28e-86 274
MsG0180001512.01.T01 MTR_7g017040 41.218 427 176 8 65 472 2 372 2.01e-85 271
MsG0180001512.01.T01 MTR_6g080680 40.000 435 169 11 66 474 3 371 7.35e-85 270
MsG0180001512.01.T01 MTR_4g102410 40.225 445 177 12 61 474 40 426 1.66e-83 268
MsG0180001512.01.T01 MTR_5g024260 40.323 434 183 13 62 473 2 381 1.71e-81 261
MsG0180001512.01.T01 MTR_8g041270 41.204 432 178 12 69 474 2 383 2.92e-81 261
MsG0180001512.01.T01 MTR_0129s0050 38.926 447 173 12 65 474 1 384 4.17e-81 260
MsG0180001512.01.T01 MTR_0054s0100 39.726 438 182 13 65 474 1 384 5.11e-81 260
MsG0180001512.01.T01 MTR_0054s0090 39.726 438 180 13 65 474 1 382 1.31e-80 259
MsG0180001512.01.T01 MTR_5g096630 40.281 427 169 11 81 474 17 390 1.55e-80 259
MsG0180001512.01.T01 MTR_2g034700 40.887 406 170 11 76 473 8 351 9.26e-80 256
MsG0180001512.01.T01 MTR_4g117550 41.007 417 154 13 79 479 14 354 3.32e-79 254
MsG0180001512.01.T01 MTR_2g021950 39.189 444 195 13 65 488 1 389 3.79e-79 255
MsG0180001512.01.T01 MTR_8g464770 40.476 420 188 10 79 490 9 374 7.72e-79 253
MsG0180001512.01.T01 MTR_5g097680 39.246 451 180 11 79 484 14 415 9.67e-79 255
MsG0180001512.01.T01 MTR_5g097220 39.729 443 163 11 72 474 4 382 1.03e-78 254
MsG0180001512.01.T01 MTR_4g028660 37.900 438 194 12 67 482 1 382 4.00e-78 252
MsG0180001512.01.T01 MTR_4g027450 37.900 438 194 12 67 482 1 382 4.00e-78 252
MsG0180001512.01.T01 MTR_4g055820 40.648 401 159 10 79 453 11 358 5.50e-78 251
MsG0180001512.01.T01 MTR_8g039500 40.695 403 166 11 76 467 6 346 1.08e-76 247
MsG0180001512.01.T01 MTR_2g066200 39.524 420 182 12 70 474 2 364 1.27e-76 248
MsG0180001512.01.T01 MTR_1g007450 40.421 428 179 16 80 487 10 381 4.13e-76 248
MsG0180001512.01.T01 MTR_4g130330 40.284 422 169 13 78 474 3 366 1.01e-75 246
MsG0180001512.01.T01 MTR_4g028680 39.070 430 186 12 67 474 1 376 1.97e-75 245
MsG0180001512.01.T01 MTR_6g461660 40.144 416 174 13 79 474 16 376 2.06e-75 245
MsG0180001512.01.T01 MTR_2g066170 39.225 413 181 10 76 474 6 362 3.25e-75 244
MsG0180001512.01.T01 MTR_2g065860 38.293 410 185 10 79 474 9 364 5.97e-75 244
MsG0180001512.01.T01 MTR_8g033140 37.111 450 186 12 65 474 1 393 7.14e-75 244
MsG0180001512.01.T01 MTR_6g090180 38.391 435 192 12 65 474 31 414 1.20e-74 244
MsG0180001512.01.T01 MTR_2g104340 39.623 424 165 12 80 474 12 373 1.36e-74 243
MsG0180001512.01.T01 MTR_5g097850 51.154 260 76 4 224 474 5 222 7.82e-74 236
MsG0180001512.01.T01 MTR_6g079810 40.233 430 172 12 76 474 64 439 7.83e-74 243
MsG0180001512.01.T01 MTR_2g021770 38.785 428 171 11 81 473 11 382 7.96e-74 241
MsG0180001512.01.T01 MTR_4g028510 37.955 440 194 14 67 482 1 385 8.52e-74 243
MsG0180001512.01.T01 MTR_1g007410 38.356 438 184 12 61 473 5 381 9.50e-74 241
MsG0180001512.01.T01 MTR_0134s0060 36.111 432 186 9 80 474 10 388 1.14e-73 241
MsG0180001512.01.T01 MTR_7g111700 40.048 417 143 13 79 474 12 342 1.15e-73 239
MsG0180001512.01.T01 MTR_5g097350 39.949 393 174 11 91 474 25 364 1.75e-73 240
MsG0180001512.01.T01 MTR_6g079770 36.726 452 184 13 80 491 15 404 3.15e-73 240
MsG0180001512.01.T01 MTR_2g104280 38.679 424 176 14 80 473 12 381 4.99e-73 239
MsG0180001512.01.T01 MTR_4g028750 37.529 437 196 14 67 482 1 381 9.03e-73 238
MsG0180001512.01.T01 MTR_8g046310 40.194 413 181 14 67 473 1 353 4.37e-72 236
MsG0180001512.01.T01 MTR_7g079640 38.575 407 171 12 81 474 12 352 5.86e-72 236
MsG0180001512.01.T01 MTR_8g467670 37.530 413 189 12 76 479 8 360 6.03e-72 236
MsG0180001512.01.T01 MTR_5g072060 36.301 438 171 11 80 488 4 362 2.40e-71 241
MsG0180001512.01.T01 MTR_0831s0010 37.560 418 192 11 69 474 37 397 2.44e-71 235
MsG0180001512.01.T01 MTR_1g053510 38.326 454 192 13 67 488 1 398 4.33e-71 234
MsG0180001512.01.T01 MTR_5g091140 39.709 413 159 11 81 472 47 390 1.01e-70 234
MsG0180001512.01.T01 MTR_2g066150 38.780 410 161 15 76 474 6 336 1.10e-70 232
MsG0180001512.01.T01 MTR_6g478230 38.799 433 169 15 72 474 17 383 1.72e-70 233
MsG0180001512.01.T01 MTR_2g064950 36.792 424 182 11 74 472 2 364 2.62e-70 232
MsG0180001512.01.T01 MTR_2g065840 35.080 439 207 11 69 488 2 381 3.89e-70 231
MsG0180001512.01.T01 MTR_8g032980 36.923 455 180 12 67 473 1 396 7.09e-69 229
MsG0180001512.01.T01 MTR_5g074850 39.780 455 163 22 61 474 16 400 7.13e-69 240
MsG0180001512.01.T01 MTR_8g013000 37.129 404 181 11 74 456 2 353 1.77e-68 228
MsG0180001512.01.T01 MTR_8g064890 37.530 421 193 12 67 474 1 364 1.81e-68 227
MsG0180001512.01.T01 MTR_8g036970 35.870 460 175 14 86 528 2 358 2.79e-67 223
MsG0180001512.01.T01 MTR_5g075370 38.776 441 158 20 78 474 4 376 1.15e-66 232
MsG0180001512.01.T01 MTR_1g007430 38.860 386 146 12 93 456 7 324 1.46e-66 220
MsG0180001512.01.T01 MTR_5g092850 36.547 446 177 16 65 474 1 376 3.41e-66 221
MsG0180001512.01.T01 MTR_8g033110 36.585 451 181 14 67 473 1 390 1.74e-65 220
MsG0180001512.01.T01 MTR_7g016930 39.175 388 157 7 79 456 13 331 1.82e-65 217
MsG0180001512.01.T01 MTR_4g014370 36.092 435 180 15 79 473 8 384 2.68e-63 214
MsG0180001512.01.T01 MTR_8g063330 36.650 412 168 13 70 453 2 348 3.41e-62 213
MsG0180001512.01.T01 MTR_5g097820 42.105 304 130 6 67 366 8 269 3.43e-62 206
MsG0180001512.01.T01 MTR_8g046200 36.222 450 193 15 67 474 1 398 8.70e-62 210
MsG0180001512.01.T01 MTR_8g033090 35.927 437 182 12 77 473 9 387 1.77e-61 209
MsG0180001512.01.T01 MTR_6g090250 35.202 446 179 15 100 491 29 418 4.14e-60 206
MsG0180001512.01.T01 MTR_5g074800 36.649 382 151 12 121 473 1 320 5.95e-60 202
MsG0180001512.01.T01 MTR_8g018110 34.753 446 178 15 67 473 1 372 1.24e-59 204
MsG0180001512.01.T01 MTR_8g046220 36.242 447 175 15 67 471 1 379 1.21e-58 201
MsG0180001512.01.T01 MTR_4g007940 37.903 372 147 14 131 474 3 318 1.91e-57 196
MsG0180001512.01.T01 MTR_5g092860 35.922 412 145 14 100 474 20 349 5.87e-55 190
MsG0180001512.01.T01 MTR_5g064700 35.572 402 175 15 121 484 1 356 4.53e-54 188
MsG0180001512.01.T01 MTR_8g064790 34.243 403 161 12 76 473 9 312 9.34e-53 183
MsG0180001512.01.T01 MTR_6g078010 36.243 378 152 13 119 474 1 311 3.13e-51 180
MsG0180001512.01.T01 MTR_1g050308 34.053 417 161 12 63 474 19 326 4.36e-51 179
MsG0180001512.01.T01 MTR_6g079790 39.298 285 141 11 218 474 5 285 3.51e-47 167
MsG0180001512.01.T01 MTR_1g050312 36.533 323 131 9 159 474 16 271 3.81e-47 167
MsG0180001512.01.T01 MTR_6g004450 45.540 213 91 6 286 487 52 250 7.57e-47 166
MsG0180001512.01.T01 MTR_5g043605 41.502 253 128 8 237 478 77 320 1.19e-46 168
MsG0180001512.01.T01 MTR_8g063370 30.825 412 204 14 78 473 20 366 1.38e-45 166
MsG0180001512.01.T01 MTR_8g063310 30.986 426 206 18 70 473 13 372 3.23e-44 162
MsG0180001512.01.T01 MTR_6g087620 40.074 272 117 9 222 474 20 264 5.25e-44 158
MsG0180001512.01.T01 MTR_2g021920 42.308 234 104 7 282 488 36 265 9.86e-44 157
MsG0180001512.01.T01 MTR_7g104710 34.254 362 145 12 65 411 1 284 1.05e-43 159
MsG0180001512.01.T01 MTR_5g097540 57.447 141 57 2 316 456 96 233 1.89e-43 157
MsG0180001512.01.T01 MTR_5g022850 37.234 282 135 10 222 473 60 329 5.10e-43 157
MsG0180001512.01.T01 MTR_8g033000 32.658 395 169 13 119 473 1 338 5.62e-43 158
MsG0180001512.01.T01 MTR_5g037190 35.463 313 132 7 65 369 1 251 3.07e-42 152
MsG0180001512.01.T01 MTR_6g090260 47.027 185 87 5 296 474 59 238 4.22e-42 152
MsG0180001512.01.T01 MTR_6g090260 52.174 69 32 1 64 131 2 70 5.81e-12 66.6
MsG0180001512.01.T01 MTR_3g028060 75.490 102 25 0 475 576 208 309 5.38e-42 154
MsG0180001512.01.T01 MTR_3g034370 72.381 105 29 0 475 579 174 278 9.12e-42 152
MsG0180001512.01.T01 MTR_5g023970 49.080 163 78 3 316 474 10 171 1.01e-41 149
MsG0180001512.01.T01 MTR_5g068490 39.200 250 132 10 227 473 21 253 1.93e-41 151
MsG0180001512.01.T01 MTR_5g066420 55.882 136 53 2 346 474 1 136 1.04e-40 145
MsG0180001512.01.T01 MTR_5g096070 41.589 214 47 5 79 291 23 159 2.67e-39 141
MsG0180001512.01.T01 MTR_7g023800 31.667 360 167 10 121 474 1 287 7.61e-39 145
MsG0180001512.01.T01 MTR_5g096920 54.478 134 58 2 346 478 1 132 2.60e-38 138
MsG0180001512.01.T01 MTR_1g076810 35.689 283 117 6 65 337 1 228 4.24e-38 149
MsG0180001512.01.T01 MTR_5g076490 34.627 335 144 9 159 473 31 310 4.00e-37 141
MsG0180001512.01.T01 MTR_5g094350 34.783 322 144 10 76 383 7 276 1.19e-36 138
MsG0180001512.01.T01 MTR_2g104260 36.111 324 120 12 80 374 12 277 3.13e-36 137
MsG0180001512.01.T01 MTR_6g070880 33.681 288 111 9 79 340 29 262 9.51e-36 135
MsG0180001512.01.T01 MTR_6g071420 33.681 288 111 9 79 340 29 262 9.51e-36 135
MsG0180001512.01.T01 MTR_6g009350 42.632 190 90 7 286 461 11 195 1.02e-35 133
MsG0180001512.01.T01 MTR_8g035690 37.402 254 115 10 259 473 30 278 2.06e-35 135
MsG0180001512.01.T01 MTR_3g034540 62.857 105 39 0 472 576 19 123 4.61e-35 128
MsG0180001512.01.T01 MTR_1g098180 36.626 243 121 8 253 478 120 346 1.03e-34 135
MsG0180001512.01.T01 MTR_1g098180 50.000 112 45 3 65 173 1 104 1.27e-21 97.4
MsG0180001512.01.T01 MTR_8g073570 40.553 217 73 7 80 294 8 170 2.54e-34 128
MsG0180001512.01.T01 MTR_3g034410 69.388 98 24 1 475 572 172 263 3.06e-34 131
MsG0180001512.01.T01 MTR_3g018750 36.481 233 111 6 283 484 3 229 5.92e-34 129
MsG0180001512.01.T01 MTR_5g098950 37.441 211 97 5 255 456 90 274 1.10e-33 129
MsG0180001512.01.T01 MTR_4g016000 42.584 209 82 9 258 448 2 190 4.03e-33 126
MsG0180001512.01.T01 MTR_7g019250 41.584 202 89 6 286 474 227 412 5.60e-33 132
MsG0180001512.01.T01 MTR_8g063350 32.734 278 156 11 213 472 55 319 1.42e-32 128
MsG0180001512.01.T01 MTR_5g096050 58.716 109 32 2 346 441 1 109 2.30e-32 121
MsG0180001512.01.T01 MTR_6g465360 33.977 259 100 7 58 298 35 240 3.33e-32 126
MsG0180001512.01.T01 MTR_5g097660 63.441 93 33 1 382 473 3 95 9.98e-32 119
MsG0180001512.01.T01 MTR_5g091150 43.956 182 70 6 227 406 35 186 1.40e-31 121
MsG0180001512.01.T01 MTR_5g097650 39.423 208 60 6 77 284 17 158 2.72e-30 116
MsG0180001512.01.T01 MTR_5g049560 40.761 184 68 3 119 298 1 147 4.55e-30 119
MsG0180001512.01.T01 MTR_6g079730 51.327 113 55 0 362 474 14 126 1.67e-29 114
MsG0180001512.01.T01 MTR_1g115205 33.007 306 104 13 64 353 16 236 1.97e-27 111
MsG0180001512.01.T01 MTR_3g030620 45.070 142 66 5 342 474 19 157 8.16e-27 107
MsG0180001512.01.T01 MTR_5g086780 48.571 105 54 0 370 474 124 228 1.44e-26 108
MsG0180001512.01.T01 MTR_5g086780 67.347 49 13 1 80 125 14 62 1.87e-11 64.7
MsG0180001512.01.T01 MTR_4g014370 37.209 172 91 5 312 473 148 312 3.51e-26 110
MsG0180001512.01.T01 MTR_4g014370 43.750 144 62 5 79 218 19 147 8.63e-20 91.3
MsG0180001512.01.T01 MTR_7g017120 42.331 163 89 2 316 474 80 241 2.66e-25 105
MsG0180001512.01.T01 MTR_7g017150 41.718 163 90 2 316 474 81 242 6.64e-25 104
MsG0180001512.01.T01 MTR_1g047080 28.415 366 145 15 119 474 1 259 1.84e-23 101
MsG0180001512.01.T01 MTR_7g101200 35.321 218 99 11 253 455 38 228 1.12e-22 97.4
MsG0180001512.01.T01 MTR_5g036250 54.321 81 37 0 394 474 8 88 1.43e-21 92.8
MsG0180001512.01.T01 MTR_8g041440 51.000 100 26 1 79 178 12 88 2.43e-21 90.1
MsG0180001512.01.T01 MTR_2g022020 44.898 98 54 0 377 474 1 98 2.17e-20 87.4
MsG0180001512.01.T01 MTR_2g028140 23.059 438 251 15 82 491 5 384 9.43e-19 89.4
MsG0180001512.01.T01 MTR_3g047580 50.685 73 36 0 70 142 2 74 4.19e-18 81.3
MsG0180001512.01.T01 MTR_3g019290 45.690 116 55 4 67 181 1 109 8.24e-18 82.0
MsG0180001512.01.T01 MTR_1g037540 26.173 405 220 18 85 460 8 362 4.09e-16 80.9
MsG0180001512.01.T01 MTR_1g050305 55.556 81 31 2 399 474 21 101 8.28e-16 75.1
MsG0180001512.01.T01 MTR_3g097190 55.172 58 26 0 414 471 24 81 2.04e-15 75.1
MsG0180001512.01.T01 MTR_0003s0580 39.474 114 66 2 465 577 202 313 4.00e-15 77.0
MsG0180001512.01.T01 MTR_3g007360 34.123 211 107 12 237 433 146 338 4.82e-15 77.8
MsG0180001512.01.T01 MTR_6g087660 49.383 81 39 1 65 143 1 81 2.98e-14 72.0
MsG0180001512.01.T01 MTR_3g058480 31.847 157 90 5 237 386 144 290 3.77e-14 74.3
MsG0180001512.01.T01 MTR_2g026010 24.839 310 200 11 237 521 91 392 4.69e-14 74.7
MsG0180001512.01.T01 MTR_2g031720 23.077 273 176 11 237 485 97 359 6.23e-14 74.3
MsG0180001512.01.T01 MTR_3g047540 44.872 78 41 2 315 390 4 81 1.45e-13 67.0
MsG0180001512.01.T01 MTR_6g046320 25.000 396 238 17 82 456 39 396 1.83e-13 73.2
MsG0180001512.01.T01 MTR_3g083010 21.882 457 252 16 77 492 1 393 2.07e-13 72.8
MsG0180001512.01.T01 MTR_0627s0010 25.532 376 209 19 82 436 38 363 4.54e-13 71.6
MsG0180001512.01.T01 MTR_4g094400 36.220 127 54 3 342 452 6 121 6.49e-13 66.2
MsG0180001512.01.T01 MTR_2g031640 25.899 278 162 14 237 485 93 355 1.28e-12 70.1
MsG0180001512.01.T01 MTR_6g027910 26.271 236 140 9 239 460 114 329 1.38e-12 70.1
MsG0180001512.01.T01 MTR_2g028110 22.603 292 197 12 220 492 76 357 1.63e-12 69.7
MsG0180001512.01.T01 MTR_5g097730 71.795 39 11 0 420 458 15 53 2.41e-12 62.4
MsG0180001512.01.T01 MTR_1g082540 81.081 37 7 0 1 37 1 37 3.14e-12 63.2
MsG0180001512.01.T01 MTR_3g036335 25.610 246 153 11 222 460 127 349 3.15e-12 68.9
MsG0180001512.01.T01 MTR_3g034160 26.500 200 121 6 251 434 117 306 4.23e-12 68.6
MsG0180001512.01.T01 MTR_6g027890 25.316 316 168 14 225 502 30 315 5.68e-12 67.8
MsG0180001512.01.T01 MTR_3g011060 28.365 208 122 8 237 433 133 324 7.00e-12 67.8
MsG0180001512.01.T01 MTR_0064s0160 22.353 425 235 16 70 464 30 389 7.40e-12 68.2
MsG0180001512.01.T01 MTR_4g118370 29.952 207 106 8 256 452 34 211 7.69e-12 66.2
MsG0180001512.01.T01 MTR_0115s0020 24.009 429 224 17 77 472 5 364 8.03e-12 67.8
MsG0180001512.01.T01 MTR_8g041490 39.474 114 34 3 65 178 1 79 9.90e-12 62.4
MsG0180001512.01.T01 MTR_1g068860 23.985 271 175 10 237 485 92 353 1.10e-11 67.0
MsG0180001512.01.T01 MTR_0667s0010 24.242 297 186 12 222 485 82 372 1.44e-11 67.0
MsG0180001512.01.T01 MTR_5g021880 23.706 367 238 11 80 429 37 378 1.47e-11 67.4
MsG0180001512.01.T01 MTR_5g021880 23.706 367 238 11 80 429 37 378 1.54e-11 67.4
MsG0180001512.01.T01 MTR_5g021880 23.706 367 238 11 80 429 37 378 1.59e-11 67.4
MsG0180001512.01.T01 MTR_3g006670 25.737 373 183 16 82 438 49 343 1.59e-11 67.0
MsG0180001512.01.T01 MTR_5g049520 80.556 36 7 0 438 473 22 57 2.52e-11 62.8
MsG0180001512.01.T01 MTR_3g036430 25.234 214 137 7 222 433 81 273 3.18e-11 65.5
MsG0180001512.01.T01 MTR_5g070080 27.411 197 125 6 250 437 134 321 3.99e-11 65.5
MsG0180001512.01.T01 MTR_2g078900 27.230 213 115 9 233 433 129 313 5.26e-11 65.1
MsG0180001512.01.T01 MTR_3g034130 24.314 255 159 8 253 482 119 364 5.87e-11 64.7
MsG0180001512.01.T01 MTR_4g023120 28.272 191 123 6 249 433 107 289 8.93e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001512.01.T01 AT3G06240 24.752 404 252 17 82 474 36 398 8.47e-14 74.3
MsG0180001512.01.T01 AT3G17490 28.452 239 138 11 226 453 95 311 1.29e-11 67.0

Find 119 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTATTTGTATTCTCTTTC+TGG 0.078612 1:+22426805 MsG0180001512.01.T01:CDS
CCTTGCCTCGATCATCTTAT+TGG 0.174104 1:+22427645 MsG0180001512.01.T01:CDS
GTGTGCCATCTGATCAATTT+TGG 0.179842 1:+22427265 MsG0180001512.01.T01:CDS
AACAATTTACATTTCAGTTT+TGG 0.202229 1:+22427569 MsG0180001512.01.T01:CDS
TTGAAAGCCACCAACTAATT+TGG 0.206109 1:-22428843 None:intergenic
ACACGATTAGGGTTAAATTT+TGG 0.252670 1:-22427246 None:intergenic
AATACAAATAATAATAAATT+TGG 0.260490 1:-22426795 None:intergenic
TGCCTGAGAATAATCCTTCT+TGG 0.261563 1:+22427142 MsG0180001512.01.T01:CDS
CAGATTTCTCTGAAATTTCT+TGG 0.274346 1:-22428744 None:intergenic
TGATCAGATGGCACACGATT+AGG 0.277260 1:-22427258 None:intergenic
AACAGAAACAAATACGTATT+TGG 0.278603 1:+22427401 MsG0180001512.01.T01:CDS
AAACGCTTCAAAGATTTAAA+AGG 0.279731 1:-22426619 None:intergenic
CTCTGCAAATATGACAGTTT+TGG 0.311579 1:+22425797 None:intergenic
CGCATCGTCAAATGTTGTTC+AGG 0.342248 1:+22426543 MsG0180001512.01.T01:CDS
TAATCCTTCTTGGTATGTTT+CGG 0.353019 1:+22427152 MsG0180001512.01.T01:CDS
TTTGTTGCTCATCTTGTTGA+TGG 0.355796 1:+22428698 MsG0180001512.01.T01:CDS
CTCTTATGTAGATGACAGTT+TGG 0.358287 1:+22427470 MsG0180001512.01.T01:CDS
AACAAGGGTTGTCAAATAAG+AGG 0.367149 1:-22426666 None:intergenic
CTCGATCATCTTATTGGAGC+TGG 0.375804 1:+22427651 MsG0180001512.01.T01:CDS
GATCAGATGGCACACGATTA+GGG 0.376900 1:-22427257 None:intergenic
AGAATAGAGTCAAATTAGAT+TGG 0.387136 1:+22426839 MsG0180001512.01.T01:CDS
ACTTTGCTGATGTTAACAAA+TGG 0.407749 1:+22425853 MsG0180001512.01.T01:CDS
GGTATTCTTTGTCTAAAAGC+TGG 0.408632 1:+22427054 MsG0180001512.01.T01:CDS
TCGTCATCATCCTCCTCATC+TGG 0.408698 1:-22426970 None:intergenic
CAAACTGTCATCTACATAAG+AGG 0.411595 1:-22427469 None:intergenic
GTCATCTACATAAGAGGGTA+TGG 0.414415 1:-22427463 None:intergenic
CCATCATCATCCTCTTGAAA+TGG 0.419953 1:-22427000 None:intergenic
CTTTGCTGATGTTAACAAAT+GGG 0.426171 1:+22425854 MsG0180001512.01.T01:CDS
AAAGAATACCATTAAAACTA+AGG 0.435281 1:-22427041 None:intergenic
AGTGGTCTTCTCAAAACTAT+AGG 0.436461 1:-22426916 None:intergenic
AGTTTGAATTCGTTGGTAGT+TGG 0.439341 1:-22427111 None:intergenic
ACATCCTCGGTCAAAGCCTT+TGG 0.445894 1:-22426862 None:intergenic
TTAATAACATCAAGTCTCTC+TGG 0.447534 1:-22427506 None:intergenic
GCTTGGTTTGATTTAAGTAC+CGG 0.449407 1:+22427717 MsG0180001512.01.T01:CDS
GTTAACATCAGCAAAGTGAA+TGG 0.457251 1:-22425847 None:intergenic
AAATTTGGCGTCTCTCTTGT+GGG 0.462152 1:-22426780 None:intergenic
ATAGCCTACGCAGTAACTCT+TGG 0.465302 1:+22427295 MsG0180001512.01.T01:CDS
AGAGACTTGATGTTATTAAA+CGG 0.466511 1:+22427510 MsG0180001512.01.T01:CDS
TGGACTAAGCACTTCGTTGT+TGG 0.468288 1:+22427615 MsG0180001512.01.T01:CDS
CCATTTCAAGAGGATGATGA+TGG 0.471596 1:+22427000 MsG0180001512.01.T01:CDS
TCGGCCGATCATTATCAAAT+CGG 0.473206 1:+22427171 MsG0180001512.01.T01:CDS
TAAATTTGGCGTCTCTCTTG+TGG 0.473870 1:-22426781 None:intergenic
CTGATGTTAACAAATGGGAC+CGG 0.480323 1:+22425859 MsG0180001512.01.T01:CDS
TATCAAGTATTCAAACTTCG+AGG 0.480867 1:+22425977 MsG0180001512.01.T01:CDS
TGTCTAAAAGCTGGATTCTA+CGG 0.481457 1:+22427063 MsG0180001512.01.T01:CDS
TGTATTCTCTTTCTGGTGAG+AGG 0.484321 1:+22426812 MsG0180001512.01.T01:CDS
TCTTATTGGAGCTGGGAAGA+AGG 0.487007 1:+22427659 MsG0180001512.01.T01:CDS
CGTAATCGAGTGTACTTGAA+TGG 0.494817 1:+22427357 MsG0180001512.01.T01:CDS
AAGATGATCGAGGCAAGGTA+AGG 0.502901 1:-22427640 None:intergenic
GGATGTCTATCGACTTCAAA+AGG 0.503568 1:+22426879 MsG0180001512.01.T01:CDS
AGGAATGAGTTTGAATTCGT+TGG 0.503942 1:-22427118 None:intergenic
AAAGTGAATGGAATCGAGTA+AGG 0.505018 1:-22425835 None:intergenic
GCTAAGCCATACATGGAGTT+GGG 0.506012 1:+22428563 MsG0180001512.01.T01:intron
CATCATCTTCTCTAAGATGT+AGG 0.507693 1:-22426756 None:intergenic
AACTCGGTGTAAAGGAATCA+TGG 0.507915 1:+22427595 MsG0180001512.01.T01:CDS
TTTGGGTGAACTCGGTGTAA+AGG 0.508652 1:+22427587 MsG0180001512.01.T01:CDS
CTTATTGGAGCTGGGAAGAA+GGG 0.513717 1:+22427660 MsG0180001512.01.T01:CDS
AATTCTAAATCACCATGGCC+TGG 0.515306 1:-22425930 None:intergenic
GCGCAAGCTCTATCGTCTCC+AGG 0.516194 1:+22425912 MsG0180001512.01.T01:CDS
TGCTAAGCCATACATGGAGT+TGG 0.517723 1:+22428562 MsG0180001512.01.T01:intron
TACCAAGAAGGATTATTCTC+AGG 0.518019 1:-22427144 None:intergenic
ATCTCCCAAAATTGATCAGA+TGG 0.520160 1:-22427270 None:intergenic
ACTTGAATGGAGTGTCTCAT+TGG 0.521299 1:+22427370 MsG0180001512.01.T01:CDS
CAGAGAAATCTGTGCGAGGT+AGG 0.525539 1:+22428755 MsG0180001512.01.T01:CDS
TTGAGAAGACCACTGGTACC+AGG 0.527358 1:+22426925 MsG0180001512.01.T01:CDS
ATAGAGAAGACAATATCATC+TGG 0.531548 1:-22426583 None:intergenic
TGATGATATAAGCACGGAGG+TGG 0.531812 1:+22428787 MsG0180001512.01.T01:CDS
CTTGGTTTGATTTAAGTACC+GGG 0.533853 1:+22427718 MsG0180001512.01.T01:CDS
CGGAGAACCAAATTAGTTGG+TGG 0.538146 1:+22428836 MsG0180001512.01.T01:CDS
AAGAATACCATTAAAACTAA+GGG 0.540846 1:-22427040 None:intergenic
AGAGAAATCTGTGCGAGGTA+GGG 0.541376 1:+22428756 MsG0180001512.01.T01:CDS
ACCGGGATGATCGATGAGAT+TGG 0.546732 1:+22427735 MsG0180001512.01.T01:CDS
ACTCGGAGAACCAAATTAGT+TGG 0.547989 1:+22428833 MsG0180001512.01.T01:CDS
TCGATCATCTTATTGGAGCT+GGG 0.549271 1:+22427652 MsG0180001512.01.T01:CDS
TTGAATGTGTACGTAAACCT+TGG 0.554355 1:+22426641 MsG0180001512.01.T01:CDS
GCTCTATCGTCTCCAGGCCA+TGG 0.562663 1:+22425918 MsG0180001512.01.T01:CDS
TGGTGATGCTAAGCCATACA+TGG 0.563384 1:+22428556 MsG0180001512.01.T01:intron
TCCTCATCTGGATGAACATG+TGG 0.576591 1:-22426958 None:intergenic
CGGTACATAGTCATGAAACA+AGG 0.578691 1:-22426682 None:intergenic
GAAGGATTATTCTCAGGCAA+AGG 0.581560 1:-22427138 None:intergenic
ACAAGGGTTGTCAAATAAGA+GGG 0.590344 1:-22426665 None:intergenic
TGAATGGAGTGTCTCATTGG+TGG 0.594828 1:+22427373 MsG0180001512.01.T01:CDS
GCGAAACGAGAGTTGTATTG+TGG 0.596166 1:+22427085 MsG0180001512.01.T01:CDS
ACTGAATTTGAGATCAAGCG+TGG 0.597743 1:-22428621 None:intergenic
TCATCTACATAAGAGGGTAT+GGG 0.599356 1:-22427462 None:intergenic
ACATGTGGAACAAACCATCC+TGG 0.600545 1:-22426943 None:intergenic
TATTTGATTATGTGATGACT+CGG 0.601865 1:+22428816 MsG0180001512.01.T01:CDS
TTGGCCAAAGGCTTTGACCG+AGG 0.603491 1:+22426858 MsG0180001512.01.T01:CDS
AGATGATCGAGGCAAGGTAA+GGG 0.608476 1:-22427639 None:intergenic
ACCAATCTCATCGATCATCC+CGG 0.608557 1:-22427736 None:intergenic
AAACTGTCATCTACATAAGA+GGG 0.610107 1:-22427468 None:intergenic
TTGCGCTAGAGTAGCTGAGT+CGG 0.616283 1:-22425895 None:intergenic
TCTTCCAAGAGTTACTGCGT+AGG 0.620101 1:-22427299 None:intergenic
ACATGTTCATCCAGATGAGG+AGG 0.621711 1:+22426960 MsG0180001512.01.T01:CDS
GAATGGAGTGTCTCATTGGT+GGG 0.622509 1:+22427374 MsG0180001512.01.T01:CDS
ATAACCGATTTGATAATGAT+CGG 0.624831 1:-22427175 None:intergenic
GATATTTGATGATATAAGCA+CGG 0.625089 1:+22428781 MsG0180001512.01.T01:CDS
TCCACATGTTCATCCAGATG+AGG 0.627843 1:+22426957 MsG0180001512.01.T01:CDS
TTGAAGTCGATAGACATCCT+CGG 0.634485 1:-22426875 None:intergenic
TTGTCAAATAAGAGGGACCA+AGG 0.634881 1:-22426658 None:intergenic
CTGAACAACATTTGACGATG+CGG 0.637893 1:-22426542 None:intergenic
AGTCAAATTAGATTGGCCAA+AGG 0.643209 1:+22426846 MsG0180001512.01.T01:CDS
CGACGACGGACCATTTCAAG+AGG 0.643402 1:+22426990 MsG0180001512.01.T01:CDS
GGTGTCGTTTGACTTCTGCA+AGG 0.643769 1:+22427422 MsG0180001512.01.T01:CDS
GAGGAGGATGATGACGACGA+CGG 0.644220 1:+22426976 MsG0180001512.01.T01:CDS
AGTCGGACTCGAAGACAACC+CGG 0.645689 1:-22425878 None:intergenic
CCAATAAGATGATCGAGGCA+AGG 0.646718 1:-22427645 None:intergenic
AGAATACCATTAAAACTAAG+GGG 0.648935 1:-22427039 None:intergenic
GAAGACCACTGGTACCAGGA+TGG 0.650110 1:+22426929 MsG0180001512.01.T01:CDS
TGATGTTAACAAATGGGACC+GGG 0.651060 1:+22425860 MsG0180001512.01.T01:CDS
ACAAACCATCCTGGTACCAG+TGG 0.656773 1:-22426934 None:intergenic
TCGGCCGAAACATACCAAGA+AGG 0.660122 1:-22427156 None:intergenic
GCATAAATTCTAAATCACCA+TGG 0.669209 1:-22425935 None:intergenic
GTATCATCATAATAAGAACA+TGG 0.689910 1:-22426724 None:intergenic
CAGCTCCAATAAGATGATCG+AGG 0.696833 1:-22427650 None:intergenic
ATTTCAGAGAAATCTGTGCG+AGG 0.700680 1:+22428751 MsG0180001512.01.T01:CDS
GGTACATAGTCATGAAACAA+GGG 0.721899 1:-22426681 None:intergenic
GTCTTGTTAAAAGATAGTTG+CGG 0.724702 1:-22426702 None:intergenic
ATTTGATGATATAAGCACGG+AGG 0.777148 1:+22428784 MsG0180001512.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTATTATTATATATA+AGG + Chr1:22426441-22426460 MsG0180001512.01.T01:intron 0.0%
!!! TTATACCAATATTTTTTTTT+TGG - Chr1:22426262-22426281 None:intergenic 10.0%
!! AACAATAGTTTATAATTAAG+TGG + Chr1:22428149-22428168 MsG0180001512.01.T01:intron 15.0%
!! AATTCTTAATATCAAAGATA+TGG - Chr1:22427925-22427944 None:intergenic 15.0%
!! ATTATACAATTAACTTACTT+TGG + Chr1:22428409-22428428 MsG0180001512.01.T01:intron 15.0%
!! ATTCTTAATATCAAAGATAT+GGG - Chr1:22427924-22427943 None:intergenic 15.0%
!! TATATATGATTATGTCTATT+AGG + Chr1:22427861-22427880 MsG0180001512.01.T01:intron 15.0%
!!! CTTTATCAATTTTATTTTGA+AGG + Chr1:22427948-22427967 MsG0180001512.01.T01:intron 15.0%
!! AAAGAATACCATTAAAACTA+AGG - Chr1:22427044-22427063 None:intergenic 20.0%
!! AAGAATACCATTAAAACTAA+GGG - Chr1:22427043-22427062 None:intergenic 20.0%
!! AGACATAATCATATATATTG+TGG - Chr1:22427858-22427877 None:intergenic 20.0%
!! ATGTAGAAAAAGCTTATATT+TGG + Chr1:22428069-22428088 MsG0180001512.01.T01:intron 20.0%
!! CAATGATTTGAAAATTACAA+AGG + Chr1:22428334-22428353 MsG0180001512.01.T01:intron 20.0%
!! GACATAATCATATATATTGT+GGG - Chr1:22427857-22427876 None:intergenic 20.0%
!! TATTATTTGTATTCTCTTTC+TGG + Chr1:22426805-22426824 MsG0180001512.01.T01:CDS 20.0%
!! TCCTTCCAAAAAAAAAATAT+TGG + Chr1:22426254-22426273 MsG0180001512.01.T01:intron 20.0%
!! TGTAGAAAAAGCTTATATTT+GGG + Chr1:22428070-22428089 MsG0180001512.01.T01:intron 20.0%
!!! AAATGAAGAGTATTGAATTT+TGG + Chr1:22428509-22428528 MsG0180001512.01.T01:intron 20.0%
!!! AACAATTTACATTTCAGTTT+TGG + Chr1:22427569-22427588 MsG0180001512.01.T01:CDS 20.0%
!!! AAGCTTTTTCTACATAAAAT+CGG - Chr1:22428064-22428083 None:intergenic 20.0%
!!! AATGAAGAGTATTGAATTTT+GGG + Chr1:22428510-22428529 MsG0180001512.01.T01:intron 20.0%
!!! ACAATTTACATTTCAGTTTT+GGG + Chr1:22427570-22427589 MsG0180001512.01.T01:CDS 20.0%
!!! ACCAATATTTTTTTTTTGGA+AGG - Chr1:22426258-22426277 None:intergenic 20.0%
! AAACGCTTCAAAGATTTAAA+AGG - Chr1:22426622-22426641 None:intergenic 25.0%
! AACAAAAGAAAGAAGAGAAA+AGG - Chr1:22426505-22426524 None:intergenic 25.0%
! AACAAATGTTCAATACAAGA+AGG - Chr1:22426098-22426117 None:intergenic 25.0%
! AACAGAAACAAATACGTATT+TGG + Chr1:22427401-22427420 MsG0180001512.01.T01:CDS 25.0%
! AATAAAGCAAAGATTGTTTC+TGG + Chr1:22428454-22428473 MsG0180001512.01.T01:intron 25.0%
! ACAAAAGAAAGAAGAGAAAA+GGG - Chr1:22426504-22426523 None:intergenic 25.0%
! ACTCTATCTGAAAACTTATA+TGG + Chr1:22427984-22428003 MsG0180001512.01.T01:intron 25.0%
! AGAATACCATTAAAACTAAG+GGG - Chr1:22427042-22427061 None:intergenic 25.0%
! AGAATAGAGTCAAATTAGAT+TGG + Chr1:22426839-22426858 MsG0180001512.01.T01:CDS 25.0%
! AGAGACTTGATGTTATTAAA+CGG + Chr1:22427510-22427529 MsG0180001512.01.T01:CDS 25.0%
! ATACTCTTCAGAAAAAAAGA+CGG + Chr1:22427687-22427706 MsG0180001512.01.T01:CDS 25.0%
! GAAAATAGTTAACTTTGCTT+TGG + Chr1:22426125-22426144 MsG0180001512.01.T01:intron 25.0%
! GTATCATCATAATAAGAACA+TGG - Chr1:22426727-22426746 None:intergenic 25.0%
! TATATATAAGGAAACATACG+TGG + Chr1:22426453-22426472 MsG0180001512.01.T01:intron 25.0%
! TATTTGATTATGTGATGACT+CGG + Chr1:22428816-22428835 MsG0180001512.01.T01:CDS 25.0%
! TTTGTATATATTCCTCCAAT+TGG - Chr1:22427816-22427835 None:intergenic 25.0%
!! GATATTTGATGATATAAGCA+CGG + Chr1:22428781-22428800 MsG0180001512.01.T01:CDS 25.0%
!!! ACTGCTCATCTTTTTTTTTT+AGG - Chr1:22426173-22426192 None:intergenic 25.0%
!!! ATAACCGATTTGATAATGAT+CGG - Chr1:22427178-22427197 None:intergenic 25.0%
!!! CTGCTCATCTTTTTTTTTTA+GGG - Chr1:22426172-22426191 None:intergenic 25.0%
AAACTGTCATCTACATAAGA+GGG - Chr1:22427471-22427490 None:intergenic 30.0%
ACTTTGCTGATGTTAACAAA+TGG + Chr1:22425853-22425872 MsG0180001512.01.T01:CDS 30.0%
ATAGAGAAGACAATATCATC+TGG - Chr1:22426586-22426605 None:intergenic 30.0%
CTTTGCTGATGTTAACAAAT+GGG + Chr1:22425854-22425873 MsG0180001512.01.T01:CDS 30.0%
GCATAAATTCTAAATCACCA+TGG - Chr1:22425938-22425957 None:intergenic 30.0%
TAATCCTTCTTGGTATGTTT+CGG + Chr1:22427152-22427171 MsG0180001512.01.T01:CDS 30.0%
TATCAAGTATTCAAACTTCG+AGG + Chr1:22425977-22425996 MsG0180001512.01.T01:CDS 30.0%
TGCAGTTGCTAAAATCAATT+TGG + Chr1:22428379-22428398 MsG0180001512.01.T01:intron 30.0%
TTAATAACATCAAGTCTCTC+TGG - Chr1:22427509-22427528 None:intergenic 30.0%
! ACACGATTAGGGTTAAATTT+TGG - Chr1:22427249-22427268 None:intergenic 30.0%
! CTTTTTCTACATAAAATCGG+AGG - Chr1:22428061-22428080 None:intergenic 30.0%
! GTCTTGTTAAAAGATAGTTG+CGG - Chr1:22426705-22426724 None:intergenic 30.0%
!! AGTATTGAATTTTGGGTATG+TGG + Chr1:22428517-22428536 MsG0180001512.01.T01:intron 30.0%
!! CAGATTTCTCTGAAATTTCT+TGG - Chr1:22428747-22428766 None:intergenic 30.0%
!! CTTCTTTCTTTTGTTTCAGA+TGG + Chr1:22426510-22426529 MsG0180001512.01.T01:intron 30.0%
!!! TGACAGTTTGGATTTTAACT+TGG + Chr1:22427482-22427501 MsG0180001512.01.T01:CDS 30.0%
!!! TTACATCCCCTTAGTTTTAA+TGG + Chr1:22427033-22427052 MsG0180001512.01.T01:CDS 30.0%
AAAGAAAGCCTTCTTCCAAT+TGG + Chr1:22427798-22427817 MsG0180001512.01.T01:intron 35.0%
AAAGTGAATGGAATCGAGTA+AGG - Chr1:22425838-22425857 None:intergenic 35.0%
AACAAGGGTTGTCAAATAAG+AGG - Chr1:22426669-22426688 None:intergenic 35.0%
ACAAGGGTTGTCAAATAAGA+GGG - Chr1:22426668-22426687 None:intergenic 35.0%
AGGAATGAGTTTGAATTCGT+TGG - Chr1:22427121-22427140 None:intergenic 35.0%
AGTGGTCTTCTCAAAACTAT+AGG - Chr1:22426919-22426938 None:intergenic 35.0%
ATATTCCTCCAATTGGAAGA+AGG - Chr1:22427809-22427828 None:intergenic 35.0%
ATCTCCCAAAATTGATCAGA+TGG - Chr1:22427273-22427292 None:intergenic 35.0%
ATGCATGCAAATATGCTACT+TGG + Chr1:22428238-22428257 MsG0180001512.01.T01:intron 35.0%
CAAACTGTCATCTACATAAG+AGG - Chr1:22427472-22427491 None:intergenic 35.0%
CGATTACGAATCGAAAAAGA+AGG - Chr1:22427345-22427364 None:intergenic 35.0%
CTCTTATGTAGATGACAGTT+TGG + Chr1:22427470-22427489 MsG0180001512.01.T01:CDS 35.0%
GGTACATAGTCATGAAACAA+GGG - Chr1:22426684-22426703 None:intergenic 35.0%
GGTATTCTTTGTCTAAAAGC+TGG + Chr1:22427054-22427073 MsG0180001512.01.T01:CDS 35.0%
GGTTGCTCATACTTTAACTA+AGG + Chr1:22426028-22426047 MsG0180001512.01.T01:intron 35.0%
GTTAACATCAGCAAAGTGAA+TGG - Chr1:22425850-22425869 None:intergenic 35.0%
TACCAAGAAGGATTATTCTC+AGG - Chr1:22427147-22427166 None:intergenic 35.0%
TGCTCATACTTTAACTAAGG+CGG + Chr1:22426031-22426050 MsG0180001512.01.T01:intron 35.0%
TGTCTAAAAGCTGGATTCTA+CGG + Chr1:22427063-22427082 MsG0180001512.01.T01:CDS 35.0%
TTGAAAGCCACCAACTAATT+TGG - Chr1:22428846-22428865 None:intergenic 35.0%
TTGAATGTGTACGTAAACCT+TGG + Chr1:22426641-22426660 MsG0180001512.01.T01:CDS 35.0%
TTTGTTGCTCATCTTGTTGA+TGG + Chr1:22428698-22428717 MsG0180001512.01.T01:CDS 35.0%
! AGTCAAATTAGATTGGCCAA+AGG + Chr1:22426846-22426865 MsG0180001512.01.T01:CDS 35.0%
! AGTTGGGAAAAATTGCTGTT+GGG + Chr1:22428579-22428598 MsG0180001512.01.T01:CDS 35.0%
! ATTTGATGATATAAGCACGG+AGG + Chr1:22428784-22428803 MsG0180001512.01.T01:CDS 35.0%
! ATTTTGCCCAGTACACAAAA+TGG + Chr1:22427884-22427903 MsG0180001512.01.T01:intron 35.0%
! ATTTTTCCCAACTCCATGTA+TGG - Chr1:22428572-22428591 None:intergenic 35.0%
! CTGGTAAAGAGTTGATTGTT+CGG + Chr1:22428473-22428492 MsG0180001512.01.T01:intron 35.0%
! CTTGGTTTGATTTAAGTACC+GGG + Chr1:22427718-22427737 MsG0180001512.01.T01:CDS 35.0%
! GCTTGGTTTGATTTAAGTAC+CGG + Chr1:22427717-22427736 MsG0180001512.01.T01:CDS 35.0%
! TCATCTACATAAGAGGGTAT+GGG - Chr1:22427465-22427484 None:intergenic 35.0%
! TGGTAAAGAGTTGATTGTTC+GGG + Chr1:22428474-22428493 MsG0180001512.01.T01:intron 35.0%
! TGTGCCATCTGATCAATTTT+GGG + Chr1:22427266-22427285 MsG0180001512.01.T01:CDS 35.0%
!! AGTTTGAATTCGTTGGTAGT+TGG - Chr1:22427114-22427133 None:intergenic 35.0%
!! CATCATCTTCTCTAAGATGT+AGG - Chr1:22426759-22426778 None:intergenic 35.0%
!! GTGGTCTTGTCTTGTTTATA+TGG + Chr1:22428536-22428555 MsG0180001512.01.T01:intron 35.0%
!!! ATTTCAGTTTTGGGTGAACT+CGG + Chr1:22427579-22427598 MsG0180001512.01.T01:CDS 35.0%
!!! TATAGTTTTGAGAAGACCAC+TGG + Chr1:22426918-22426937 MsG0180001512.01.T01:CDS 35.0%
AAAAAGACGGTGAACTAGCT+TGG + Chr1:22427700-22427719 MsG0180001512.01.T01:CDS 40.0%
AAATTTGGCGTCTCTCTTGT+GGG - Chr1:22426783-22426802 None:intergenic 40.0%
AATTCTAAATCACCATGGCC+TGG - Chr1:22425933-22425952 None:intergenic 40.0%
ACTCGGAGAACCAAATTAGT+TGG + Chr1:22428833-22428852 MsG0180001512.01.T01:CDS 40.0%
ATTTCAGAGAAATCTGTGCG+AGG + Chr1:22428751-22428770 MsG0180001512.01.T01:CDS 40.0%
CCATCATCATCCTCTTGAAA+TGG - Chr1:22427003-22427022 None:intergenic 40.0%
CCATTTCAAGAGGATGATGA+TGG + Chr1:22427000-22427019 MsG0180001512.01.T01:CDS 40.0%
CGGTACATAGTCATGAAACA+AGG - Chr1:22426685-22426704 None:intergenic 40.0%
CGTAATCGAGTGTACTTGAA+TGG + Chr1:22427357-22427376 MsG0180001512.01.T01:CDS 40.0%
CTGAACAACATTTGACGATG+CGG - Chr1:22426545-22426564 None:intergenic 40.0%
CTGATGTTAACAAATGGGAC+CGG + Chr1:22425859-22425878 MsG0180001512.01.T01:CDS 40.0%
GAAGGATTATTCTCAGGCAA+AGG - Chr1:22427141-22427160 None:intergenic 40.0%
GAGTTGGGAAAAATTGCTGT+TGG + Chr1:22428578-22428597 MsG0180001512.01.T01:CDS 40.0%
GATGAAGATATGTTGCTCAC+TGG - Chr1:22428108-22428127 None:intergenic 40.0%
GTCATCTACATAAGAGGGTA+TGG - Chr1:22427466-22427485 None:intergenic 40.0%
TAAATTTGGCGTCTCTCTTG+TGG - Chr1:22426784-22426803 None:intergenic 40.0%
TCGATCATCTTATTGGAGCT+GGG + Chr1:22427652-22427671 MsG0180001512.01.T01:CDS 40.0%
TCGGCCGATCATTATCAAAT+CGG + Chr1:22427171-22427190 MsG0180001512.01.T01:CDS 40.0%
TGATGTTAACAAATGGGACC+GGG + Chr1:22425860-22425879 MsG0180001512.01.T01:CDS 40.0%
TGCCTGAGAATAATCCTTCT+TGG + Chr1:22427142-22427161 MsG0180001512.01.T01:CDS 40.0%
TTGAAGTCGATAGACATCCT+CGG - Chr1:22426878-22426897 None:intergenic 40.0%
TTGGAAGAAGGCTTTCTTTG+TGG - Chr1:22427797-22427816 None:intergenic 40.0%
TTGTCAAATAAGAGGGACCA+AGG - Chr1:22426661-22426680 None:intergenic 40.0%
! AAAGAGTTGATTGTTCGGGA+AGG + Chr1:22428478-22428497 MsG0180001512.01.T01:intron 40.0%
! AAGCCACCATTTTGTGTACT+GGG - Chr1:22427893-22427912 None:intergenic 40.0%
! ACTGAATTTGAGATCAAGCG+TGG - Chr1:22428624-22428643 None:intergenic 40.0%
! ACTTGAATGGAGTGTCTCAT+TGG + Chr1:22427370-22427389 MsG0180001512.01.T01:CDS 40.0%
! GTGTGCCATCTGATCAATTT+TGG + Chr1:22427265-22427284 MsG0180001512.01.T01:CDS 40.0%
! TGTATTCTCTTTCTGGTGAG+AGG + Chr1:22426812-22426831 MsG0180001512.01.T01:CDS 40.0%
!! AACTCGGTGTAAAGGAATCA+TGG + Chr1:22427595-22427614 MsG0180001512.01.T01:CDS 40.0%
!! GGATGTCTATCGACTTCAAA+AGG + Chr1:22426879-22426898 MsG0180001512.01.T01:CDS 40.0%
AACTAAGGCGGCCTGTTATT+GGG + Chr1:22426043-22426062 MsG0180001512.01.T01:intron 45.0%
AAGATGATCGAGGCAAGGTA+AGG - Chr1:22427643-22427662 None:intergenic 45.0%
ACATGTGGAACAAACCATCC+TGG - Chr1:22426946-22426965 None:intergenic 45.0%
ACATGTTCATCCAGATGAGG+AGG + Chr1:22426960-22426979 MsG0180001512.01.T01:CDS 45.0%
ACCAATCTCATCGATCATCC+CGG - Chr1:22427739-22427758 None:intergenic 45.0%
ACTTCGAGGTGAAGTTTGTC+AGG + Chr1:22425991-22426010 MsG0180001512.01.T01:intron 45.0%
AGAGAAATCTGTGCGAGGTA+GGG + Chr1:22428756-22428775 MsG0180001512.01.T01:CDS 45.0%
AGATGATCGAGGCAAGGTAA+GGG - Chr1:22427642-22427661 None:intergenic 45.0%
ATAGCCTACGCAGTAACTCT+TGG + Chr1:22427295-22427314 MsG0180001512.01.T01:CDS 45.0%
CAGCTCCAATAAGATGATCG+AGG - Chr1:22427653-22427672 None:intergenic 45.0%
CCAATAAGATGATCGAGGCA+AGG - Chr1:22427648-22427667 None:intergenic 45.0%
CCTTGCCTCGATCATCTTAT+TGG + Chr1:22427645-22427664 MsG0180001512.01.T01:CDS 45.0%
CGCATCGTCAAATGTTGTTC+AGG + Chr1:22426543-22426562 MsG0180001512.01.T01:CDS 45.0%
CGGAGAACCAAATTAGTTGG+TGG + Chr1:22428836-22428855 MsG0180001512.01.T01:CDS 45.0%
CTCGATCATCTTATTGGAGC+TGG + Chr1:22427651-22427670 MsG0180001512.01.T01:CDS 45.0%
CTTATTGGAGCTGGGAAGAA+GGG + Chr1:22427660-22427679 MsG0180001512.01.T01:CDS 45.0%
GAAAGCCTTCTTCCAATTGG+AGG + Chr1:22427801-22427820 MsG0180001512.01.T01:intron 45.0%
GATCAGATGGCACACGATTA+GGG - Chr1:22427260-22427279 None:intergenic 45.0%
GCGAAACGAGAGTTGTATTG+TGG + Chr1:22427085-22427104 MsG0180001512.01.T01:CDS 45.0%
GCTAAGCCATACATGGAGTT+GGG + Chr1:22428563-22428582 MsG0180001512.01.T01:intron 45.0%
TAACTAAGGCGGCCTGTTAT+TGG + Chr1:22426042-22426061 MsG0180001512.01.T01:intron 45.0%
TCCACATGTTCATCCAGATG+AGG + Chr1:22426957-22426976 MsG0180001512.01.T01:CDS 45.0%
TCCTCATCTGGATGAACATG+TGG - Chr1:22426961-22426980 None:intergenic 45.0%
TCTTATTGGAGCTGGGAAGA+AGG + Chr1:22427659-22427678 MsG0180001512.01.T01:CDS 45.0%
TCTTCCAAGAGTTACTGCGT+AGG - Chr1:22427302-22427321 None:intergenic 45.0%
TGATCAGATGGCACACGATT+AGG - Chr1:22427261-22427280 None:intergenic 45.0%
TGATGATATAAGCACGGAGG+TGG + Chr1:22428787-22428806 MsG0180001512.01.T01:CDS 45.0%
TGCTAAGCCATACATGGAGT+TGG + Chr1:22428562-22428581 MsG0180001512.01.T01:intron 45.0%
TGGACTAAGCACTTCGTTGT+TGG + Chr1:22427615-22427634 MsG0180001512.01.T01:CDS 45.0%
TGTCAGGAGACAAACGAACA+TGG + Chr1:22426007-22426026 MsG0180001512.01.T01:intron 45.0%
TTGCCCAGTACACAAAATGG+TGG + Chr1:22427887-22427906 MsG0180001512.01.T01:intron 45.0%
! CAAGCCACCATTTTGTGTAC+TGG - Chr1:22427894-22427913 None:intergenic 45.0%
! GAATGGAGTGTCTCATTGGT+GGG + Chr1:22427374-22427393 MsG0180001512.01.T01:CDS 45.0%
! GATTGTTCGGGAAGGTGTAA+AGG + Chr1:22428486-22428505 MsG0180001512.01.T01:intron 45.0%
! TGAATGGAGTGTCTCATTGG+TGG + Chr1:22427373-22427392 MsG0180001512.01.T01:CDS 45.0%
! TGGTGATGCTAAGCCATACA+TGG + Chr1:22428556-22428575 MsG0180001512.01.T01:intron 45.0%
!! TTTGGGTGAACTCGGTGTAA+AGG + Chr1:22427587-22427606 MsG0180001512.01.T01:CDS 45.0%
!! AATACAAATAATAATAAATT+TGG - Chr1:22426798-22426817 None:intergenic 5.0%
!! GATAATAATAAAAAAAAATA+CGG - Chr1:22426076-22426095 None:intergenic 5.0%
!! TATATAAATAATATAGTATT+GGG + Chr1:22426356-22426375 MsG0180001512.01.T01:intron 5.0%
!! TTATATAAATAATATAGTAT+TGG + Chr1:22426355-22426374 MsG0180001512.01.T01:intron 5.0%
ACAAACCATCCTGGTACCAG+TGG - Chr1:22426937-22426956 None:intergenic 50.0%
ACATCCTCGGTCAAAGCCTT+TGG - Chr1:22426865-22426884 None:intergenic 50.0%
ACCGGGATGATCGATGAGAT+TGG + Chr1:22427735-22427754 MsG0180001512.01.T01:CDS 50.0%
CAGAGAAATCTGTGCGAGGT+AGG + Chr1:22428755-22428774 MsG0180001512.01.T01:CDS 50.0%
TCGGCCGAAACATACCAAGA+AGG - Chr1:22427159-22427178 None:intergenic 50.0%
TCGTCATCATCCTCCTCATC+TGG - Chr1:22426973-22426992 None:intergenic 50.0%
TTGAGAAGACCACTGGTACC+AGG + Chr1:22426925-22426944 MsG0180001512.01.T01:CDS 50.0%
TTGCGCTAGAGTAGCTGAGT+CGG - Chr1:22425898-22425917 None:intergenic 50.0%
! ACGGCGATTAGCCCAATAAC+AGG - Chr1:22426057-22426076 None:intergenic 50.0%
!! GGTGTCGTTTGACTTCTGCA+AGG + Chr1:22427422-22427441 MsG0180001512.01.T01:CDS 50.0%
AGTCGGACTCGAAGACAACC+CGG - Chr1:22425881-22425900 None:intergenic 55.0%
CGACGACGGACCATTTCAAG+AGG + Chr1:22426990-22427009 MsG0180001512.01.T01:CDS 55.0%
GAAGACCACTGGTACCAGGA+TGG + Chr1:22426929-22426948 MsG0180001512.01.T01:CDS 55.0%
! GAGGAGGATGATGACGACGA+CGG + Chr1:22426976-22426995 MsG0180001512.01.T01:CDS 55.0%
! TTGGCCAAAGGCTTTGACCG+AGG + Chr1:22426858-22426877 MsG0180001512.01.T01:CDS 55.0%
GCGCAAGCTCTATCGTCTCC+AGG + Chr1:22425912-22425931 MsG0180001512.01.T01:CDS 60.0%
GCTCTATCGTCTCCAGGCCA+TGG + Chr1:22425918-22425937 MsG0180001512.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 22425807 22428886 22425807 ID=MsG0180001512.01;Name=MsG0180001512.01
Chr1 mRNA 22425807 22428886 22425807 ID=MsG0180001512.01.T01;Parent=MsG0180001512.01;Name=MsG0180001512.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|0.66|1|1|3|0|579
Chr1 exon 22425807 22425998 22425807 ID=MsG0180001512.01.T01:exon:426;Parent=MsG0180001512.01.T01
Chr1 exon 22426529 22427758 22426529 ID=MsG0180001512.01.T01:exon:425;Parent=MsG0180001512.01.T01
Chr1 exon 22428569 22428886 22428569 ID=MsG0180001512.01.T01:exon:424;Parent=MsG0180001512.01.T01
Chr1 CDS 22425807 22425998 22425807 ID=MsG0180001512.01.T01:cds;Parent=MsG0180001512.01.T01
Chr1 CDS 22426529 22427758 22426529 ID=MsG0180001512.01.T01:cds;Parent=MsG0180001512.01.T01
Chr1 CDS 22428569 22428886 22428569 ID=MsG0180001512.01.T01:cds;Parent=MsG0180001512.01.T01
Gene Sequence

>MsG0180001512.01.T01

ATGACAGTTTTGGAGGAGGAGGCAGTAACCTTACTCGATTCCATTCACTTTGCTGATGTTAACAAATGGGACCGGGTTGTCTTCGAGTCCGACTCAGCTACTCTAGCGCAAGCTCTATCGTCTCCAGGCCATGGTGATTTAGAATTTATGCTATTGTTTCTAGTATTATTTATCAAGTATTCAAACTTCGAGATGGAGATGTCATCCGCATCGTCAAATGTTGTTCAGGTTAGCAGCAATCATATACCAGATGATATTGTCTTCTCTATTCTCTCTAAACTTCCTTTTAAATCTTTGAAGCGTTTTGAATGTGTACGTAAACCTTGGTCCCTCTTATTTGACAACCCTTGTTTCATGACTATGTACCGCAACTATCTTTTAACAAGACCATGTTCTTATTATGATGATACATCTCTTCTCCTACATCTTAGAGAAGATGATGACCCACAAGAGAGACGCCAAATTTATTATTATTTGTATTCTCTTTCTGGTGAGAGGTTTGAGAATAGAGTCAAATTAGATTGGCCAAAGGCTTTGACCGAGGATGTCTATCGACTTCAAAAGGAACAGTGTGTTCGTCCTATAGTTTTGAGAAGACCACTGGTACCAGGATGGTTTGTTCCACATGTTCATCCAGATGAGGAGGATGATGACGACGACGGACCATTTCAAGAGGATGATGATGGTTTTGAAATTTTACATCCCCTTAGTTTTAATGGTATTCTTTGTCTAAAAGCTGGATTCTACGGCGAAACGAGAGTTGTATTGTGGAATCCAACTACCAACGAATTCAAACTCATTCCTTTGCCTGAGAATAATCCTTCTTGGTATGTTTCGGCCGATCATTATCAAATCGGTTATGATCATGTTAAAGATGATTATAAGATGATTCGCTGCACATATTATTCTCCAAAATTTAACCCTAATCGTGTGCCATCTGATCAATTTTGGGAGATCTATAGCCTACGCAGTAACTCTTGGAAGAAAATTAATGTCGACATGACTCCTTCTTTTTCGATTCGTAATCGAGTGTACTTGAATGGAGTGTCTCATTGGTGGGATAAAACAGAAACAAATACGTATTTGGTGTCGTTTGACTTCTGCAAGGAATCTTTTATTACAACACCCATACCCTCTTATGTAGATGACAGTTTGGATTTTAACTTGGTCCAGAGAGACTTGATGTTATTAAACGGATCTGTTGCTTTTATGTTACATTATACAGAGACATCAACAATTTACATTTCAGTTTTGGGTGAACTCGGTGTAAAGGAATCATGGACTAAGCACTTCGTTGTTGGTCCCTTACCTTGCCTCGATCATCTTATTGGAGCTGGGAAGAAGGGCAATATACTCTTCAGAAAAAAAGACGGTGAACTAGCTTGGTTTGATTTAAGTACCGGGATGATCGATGAGATTGGTCCATACATGGAGTTGGGAAAAATTGCTGTTGGGTGTGAAAATTCTATAGTTTCCACGCTTGATCTCAAATTCAGTGAAAAGCTAACGAAGAAAATGCAATGCTTTAGTGAGATCAAAGAGATGAACAATTTTGTTGCTCATCTTGTTGATGGTGATGAAGTAAGAGATGATACTACCAAGAAATTTCAGAGAAATCTGTGCGAGGTAGGGAAGATATTTGATGATATAAGCACGGAGGTGGATCATCTATTTGATTATGTGATGACTCGGAGAACCAAATTAGTTGGTGGCTTTCAACTTAAGAAACAACAACAATAA
Protein sequence

>MsG0180001512.01.T01

MTVLEEEAVTLLDSIHFADVNKWDRVVFESDSATLAQALSSPGHGDLEFMLLFLVLFIKYSNFEMEMSSASSNVVQVSSNHIPDDIVFSILSKLPFKSLKRFECVRKPWSLLFDNPCFMTMYRNYLLTRPCSYYDDTSLLLHLREDDDPQERRQIYYYLYSLSGERFENRVKLDWPKALTEDVYRLQKEQCVRPIVLRRPLVPGWFVPHVHPDEEDDDDDGPFQEDDDGFEILHPLSFNGILCLKAGFYGETRVVLWNPTTNEFKLIPLPENNPSWYVSADHYQIGYDHVKDDYKMIRCTYYSPKFNPNRVPSDQFWEIYSLRSNSWKKINVDMTPSFSIRNRVYLNGVSHWWDKTETNTYLVSFDFCKESFITTPIPSYVDDSLDFNLVQRDLMLLNGSVAFMLHYTETSTIYISVLGELGVKESWTKHFVVGPLPCLDHLIGAGKKGNILFRKKDGELAWFDLSTGMIDEIGPYMELGKIAVGCENSIVSTLDLKFSEKLTKKMQCFSEIKEMNNFVAHLVDGDEVRDDTTKKFQRNLCEVGKIFDDISTEVDHLFDYVMTRRTKLVGGFQLKKQQQ*