AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680034921.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034921.01.T01 MTR_6g078010 70.255 353 78 4 54 402 1 330 7.42e-170 479
MsG0680034921.01.T01 MTR_6g079770 57.074 417 161 5 1 403 1 413 6.15e-157 449
MsG0680034921.01.T01 MTR_0054s0090 57.073 410 163 4 1 404 1 403 7.43e-156 446
MsG0680034921.01.T01 MTR_0054s0100 56.553 412 164 5 1 404 1 405 1.33e-154 442
MsG0680034921.01.T01 MTR_6g079810 58.313 415 152 8 1 403 54 459 5.27e-153 441
MsG0680034921.01.T01 MTR_0134s0060 57.284 405 157 4 10 401 5 406 2.44e-152 437
MsG0680034921.01.T01 MTR_6g086825 58.231 407 147 12 10 404 62 457 6.11e-146 422
MsG0680034921.01.T01 MTR_4g028510 57.882 406 157 10 3 403 2 398 2.74e-145 421
MsG0680034921.01.T01 MTR_6g090250 56.359 401 138 9 35 405 29 422 5.69e-144 416
MsG0680034921.01.T01 MTR_4g130330 56.061 396 156 9 14 403 4 387 1.61e-140 406
MsG0680034921.01.T01 MTR_4g028660 54.680 406 171 8 3 405 2 397 7.85e-140 405
MsG0680034921.01.T01 MTR_4g027450 54.680 406 171 8 3 405 2 397 7.85e-140 405
MsG0680034921.01.T01 MTR_6g080680 55.583 412 149 12 1 401 1 389 1.79e-137 399
MsG0680034921.01.T01 MTR_4g028680 54.321 405 171 10 3 403 2 396 5.50e-134 390
MsG0680034921.01.T01 MTR_6g478230 54.634 410 138 12 14 404 24 404 6.08e-132 385
MsG0680034921.01.T01 MTR_6g090180 50.597 419 180 9 1 405 31 436 3.57e-125 369
MsG0680034921.01.T01 MTR_4g028750 54.404 386 159 12 5 385 3 376 1.21e-124 366
MsG0680034921.01.T01 MTR_0129s0070 46.024 415 193 11 1 405 1 394 1.59e-103 312
MsG0680034921.01.T01 MTR_8g063440 44.938 405 190 10 6 403 1 379 1.20e-101 307
MsG0680034921.01.T01 MTR_5g097220 45.093 428 190 14 1 405 1 406 4.04e-101 306
MsG0680034921.01.T01 MTR_0129s0120 45.542 415 195 11 1 405 1 394 4.65e-100 303
MsG0680034921.01.T01 MTR_6g079790 54.777 314 121 10 101 403 2 305 4.96e-100 300
MsG0680034921.01.T01 MTR_7g023800 47.159 352 141 4 56 405 1 309 4.44e-98 295
MsG0680034921.01.T01 MTR_7g104710 51.976 329 108 6 1 322 1 286 2.79e-95 288
MsG0680034921.01.T01 MTR_6g087620 50.479 313 115 7 90 397 2 279 1.21e-94 285
MsG0680034921.01.T01 MTR_2g022000 42.683 410 197 9 1 386 1 396 3.22e-93 287
MsG0680034921.01.T01 MTR_2g021800 42.689 424 202 11 1 399 1 408 2.97e-92 284
MsG0680034921.01.T01 MTR_0129s0050 41.822 428 200 11 1 403 1 404 7.63e-91 280
MsG0680034921.01.T01 MTR_2g038680 42.459 431 193 14 3 403 2 407 8.17e-90 277
MsG0680034921.01.T01 MTR_6g461660 42.482 419 202 10 2 405 4 398 7.00e-89 275
MsG0680034921.01.T01 MTR_2g021950 41.646 413 211 12 1 403 1 393 8.82e-88 271
MsG0680034921.01.T01 MTR_5g037680 41.951 410 182 12 14 401 5 380 2.60e-87 272
MsG0680034921.01.T01 MTR_5g097330 41.289 419 205 12 6 405 5 401 6.53e-86 267
MsG0680034921.01.T01 MTR_5g059430 40.426 423 198 13 14 404 23 423 7.36e-86 268
MsG0680034921.01.T01 MTR_5g092850 42.718 412 196 14 12 405 11 400 1.24e-85 266
MsG0680034921.01.T01 MTR_4g102410 42.381 420 210 13 1 404 44 447 1.57e-85 268
MsG0680034921.01.T01 MTR_0831s0010 42.432 403 191 13 14 405 47 419 2.69e-85 266
MsG0680034921.01.T01 MTR_4g117550 39.806 412 196 11 1 403 1 369 4.14e-85 264
MsG0680034921.01.T01 MTR_5g097680 40.972 432 217 12 1 403 1 423 6.87e-85 265
MsG0680034921.01.T01 MTR_1g007450 41.294 402 202 11 6 393 1 382 1.35e-84 265
MsG0680034921.01.T01 MTR_8g012090 44.444 378 141 10 13 376 12 334 2.30e-84 261
MsG0680034921.01.T01 MTR_2g021770 40.618 421 203 14 9 403 4 403 2.35e-84 263
MsG0680034921.01.T01 MTR_6g090260 65.979 194 60 3 214 405 73 262 1.49e-83 256
MsG0680034921.01.T01 MTR_6g090260 61.538 91 31 3 1 88 1 90 2.01e-21 93.2
MsG0680034921.01.T01 MTR_3g011120 42.432 403 184 15 7 397 4 370 2.96e-83 259
MsG0680034921.01.T01 MTR_8g464770 41.439 403 204 8 6 403 1 376 3.68e-83 259
MsG0680034921.01.T01 MTR_5g069390 40.049 412 204 12 14 403 23 413 2.56e-82 261
MsG0680034921.01.T01 MTR_5g097350 42.105 418 196 15 1 405 1 385 2.64e-82 257
MsG0680034921.01.T01 MTR_5g096630 39.161 429 209 12 5 404 6 411 4.92e-81 255
MsG0680034921.01.T01 MTR_7g069550 40.338 414 188 14 13 399 10 391 1.02e-80 253
MsG0680034921.01.T01 MTR_2g065860 40.732 410 205 11 6 403 1 384 4.68e-80 251
MsG0680034921.01.T01 MTR_8g064890 39.401 401 209 9 11 403 10 384 9.31e-80 251
MsG0680034921.01.T01 MTR_7g017130 40.288 417 207 14 2 403 4 393 1.53e-79 251
MsG0680034921.01.T01 MTR_2g034700 41.379 406 188 13 11 405 8 374 2.21e-79 249
MsG0680034921.01.T01 MTR_6g070880 53.469 245 98 4 2 234 17 257 1.02e-78 244
MsG0680034921.01.T01 MTR_6g071420 53.469 245 98 4 2 234 17 257 1.02e-78 244
MsG0680034921.01.T01 MTR_8g467670 39.440 393 199 12 15 400 12 372 1.91e-78 248
MsG0680034921.01.T01 MTR_1g007410 39.329 417 207 13 3 399 8 398 2.23e-78 248
MsG0680034921.01.T01 MTR_7g017040 39.904 416 210 10 6 405 5 396 2.60e-78 247
MsG0680034921.01.T01 MTR_2g104340 41.205 415 196 14 7 401 4 390 5.51e-78 246
MsG0680034921.01.T01 MTR_5g074850 42.963 405 177 18 6 383 23 400 8.66e-78 258
MsG0680034921.01.T01 MTR_4g055820 42.744 379 172 11 7 363 4 359 3.86e-77 243
MsG0680034921.01.T01 MTR_7g017170 39.627 429 205 15 2 403 4 405 6.50e-77 244
MsG0680034921.01.T01 MTR_8g046310 39.552 402 189 13 15 403 14 374 7.10e-77 243
MsG0680034921.01.T01 MTR_4g014370 37.560 418 227 10 8 404 2 406 1.19e-76 243
MsG0680034921.01.T01 MTR_8g033140 37.939 427 198 11 13 401 15 412 3.33e-76 243
MsG0680034921.01.T01 MTR_2g066170 40.049 407 208 14 6 402 1 381 1.67e-75 240
MsG0680034921.01.T01 MTR_5g096910 39.573 422 203 13 1 403 1 389 2.75e-75 239
MsG0680034921.01.T01 MTR_8g041270 40.000 420 195 15 13 403 11 402 7.29e-75 239
MsG0680034921.01.T01 MTR_2g066200 38.795 415 208 14 6 404 1 385 1.18e-74 238
MsG0680034921.01.T01 MTR_2g065840 39.225 413 208 12 6 403 1 385 1.52e-74 237
MsG0680034921.01.T01 MTR_2g104280 40.964 415 194 14 13 401 10 399 5.85e-73 234
MsG0680034921.01.T01 MTR_5g075370 40.500 400 188 16 14 387 5 380 9.14e-73 243
MsG0680034921.01.T01 MTR_5g091140 39.604 404 193 12 2 390 33 400 1.70e-71 230
MsG0680034921.01.T01 MTR_1g053510 37.589 423 205 14 11 403 9 402 8.25e-71 228
MsG0680034921.01.T01 MTR_5g024260 39.250 400 195 13 11 389 16 388 1.00e-70 228
MsG0680034921.01.T01 MTR_8g046220 37.585 439 194 18 6 403 3 402 1.21e-70 228
MsG0680034921.01.T01 MTR_5g092860 40.872 367 162 12 35 383 20 349 1.98e-70 226
MsG0680034921.01.T01 MTR_8g018110 37.296 429 203 14 6 404 2 394 2.26e-70 227
MsG0680034921.01.T01 MTR_8g033090 38.356 438 201 19 6 404 2 409 2.19e-69 225
MsG0680034921.01.T01 MTR_8g039500 40.933 386 173 17 6 376 1 346 1.62e-68 220
MsG0680034921.01.T01 MTR_6g465360 52.174 230 97 2 2 218 47 276 3.52e-68 218
MsG0680034921.01.T01 MTR_6g079730 69.799 149 44 1 257 405 1 148 4.76e-68 212
MsG0680034921.01.T01 MTR_7g079640 40.212 378 179 13 16 383 12 352 1.16e-67 219
MsG0680034921.01.T01 MTR_2g066150 39.655 406 189 16 6 404 1 357 6.08e-67 217
MsG0680034921.01.T01 MTR_2g064950 37.778 405 204 15 13 398 6 381 1.73e-66 217
MsG0680034921.01.T01 MTR_5g072060 39.323 384 175 12 16 382 5 347 1.63e-65 220
MsG0680034921.01.T01 MTR_8g033110 37.783 442 202 18 5 404 2 412 1.86e-65 214
MsG0680034921.01.T01 MTR_8g046200 36.018 447 186 17 15 403 14 418 2.01e-65 215
MsG0680034921.01.T01 MTR_7g111700 38.799 433 163 15 1 403 1 361 3.72e-65 212
MsG0680034921.01.T01 MTR_8g013000 39.426 383 175 14 13 369 6 357 2.80e-64 211
MsG0680034921.01.T01 MTR_5g076490 40.608 362 173 10 54 403 1 332 2.29e-63 207
MsG0680034921.01.T01 MTR_8g032980 34.873 433 205 14 15 401 14 415 1.73e-62 207
MsG0680034921.01.T01 MTR_1g098180 35.835 413 193 14 4 401 5 360 2.11e-62 205
MsG0680034921.01.T01 MTR_8g063330 37.037 378 187 13 6 362 1 348 4.82e-62 207
MsG0680034921.01.T01 MTR_4g007940 36.517 356 182 10 67 400 2 335 1.49e-61 202
MsG0680034921.01.T01 MTR_8g063370 34.963 409 223 17 2 399 8 384 1.54e-61 204
MsG0680034921.01.T01 MTR_1g007430 38.682 349 172 10 28 365 7 324 1.64e-60 199
MsG0680034921.01.T01 MTR_5g043605 35.101 396 171 10 13 403 21 335 5.24e-59 196
MsG0680034921.01.T01 MTR_8g064790 33.911 404 191 10 4 403 2 333 6.32e-59 195
MsG0680034921.01.T01 MTR_5g074800 37.396 361 184 11 56 399 1 336 1.11e-58 195
MsG0680034921.01.T01 MTR_5g064700 36.461 373 203 12 56 403 1 364 1.23e-58 196
MsG0680034921.01.T01 MTR_7g016930 37.831 378 180 13 1 368 1 333 3.46e-56 188
MsG0680034921.01.T01 MTR_2g021920 44.262 244 113 5 175 403 34 269 3.18e-54 181
MsG0680034921.01.T01 MTR_5g068490 36.928 306 153 8 98 403 8 273 5.37e-54 181
MsG0680034921.01.T01 MTR_8g036970 36.623 385 162 13 21 395 2 314 9.41e-54 183
MsG0680034921.01.T01 MTR_6g004450 45.690 232 107 6 178 404 49 266 2.08e-53 179
MsG0680034921.01.T01 MTR_8g063310 33.252 409 226 16 13 405 18 395 2.53e-52 180
MsG0680034921.01.T01 MTR_5g096900 39.306 346 148 14 10 330 5 313 8.47e-51 174
MsG0680034921.01.T01 MTR_8g063350 34.171 398 178 15 14 398 11 337 2.19e-50 173
MsG0680034921.01.T01 MTR_5g022850 32.678 407 189 14 13 403 12 349 9.13e-50 172
MsG0680034921.01.T01 MTR_5g097850 50.526 190 76 6 221 403 63 241 1.26e-47 163
MsG0680034921.01.T01 MTR_5g086590 41.224 245 118 4 136 380 14 232 1.98e-47 162
MsG0680034921.01.T01 MTR_1g050312 39.228 311 128 12 89 388 16 276 1.88e-46 161
MsG0680034921.01.T01 MTR_3g018750 41.532 248 116 9 177 405 2 239 8.27e-46 158
MsG0680034921.01.T01 MTR_5g023970 47.739 199 88 7 211 404 6 193 4.77e-43 149
MsG0680034921.01.T01 MTR_4g094400 66.981 106 24 1 246 340 6 111 1.55e-42 145
MsG0680034921.01.T01 MTR_1g076810 39.382 259 131 8 1 251 1 241 5.82e-42 157
MsG0680034921.01.T01 MTR_6g009350 43.878 196 99 6 178 370 8 195 9.12e-42 146
MsG0680034921.01.T01 MTR_4g014370 42.932 191 99 3 215 404 153 334 1.03e-41 150
MsG0680034921.01.T01 MTR_4g014370 38.346 133 77 3 1 129 6 137 7.28e-18 84.3
MsG0680034921.01.T01 MTR_5g066420 50.340 147 71 2 250 394 1 147 1.06e-41 144
MsG0680034921.01.T01 MTR_5g097820 36.496 274 136 10 5 265 14 262 2.80e-41 147
MsG0680034921.01.T01 MTR_5g094350 36.455 299 144 10 11 292 7 276 9.26e-41 146
MsG0680034921.01.T01 MTR_8g033000 32.105 380 203 13 54 401 1 357 2.99e-40 147
MsG0680034921.01.T01 MTR_7g019250 38.168 262 120 7 129 383 186 412 1.63e-38 144
MsG0680034921.01.T01 MTR_1g050308 40.336 238 110 9 5 233 22 236 2.71e-38 141
MsG0680034921.01.T01 MTR_1g050308 41.284 109 57 3 297 403 240 343 3.66e-15 76.6
MsG0680034921.01.T01 MTR_4g016000 38.914 221 100 5 140 357 2 190 4.15e-38 137
MsG0680034921.01.T01 MTR_3g030620 45.625 160 79 4 247 401 20 176 2.13e-36 131
MsG0680034921.01.T01 MTR_5g096920 47.097 155 74 4 250 403 1 148 7.65e-36 129
MsG0680034921.01.T01 MTR_5g098950 37.391 230 98 6 137 365 90 274 1.12e-35 132
MsG0680034921.01.T01 MTR_8g035690 36.576 257 113 7 178 401 58 297 1.49e-34 130
MsG0680034921.01.T01 MTR_5g097660 51.724 116 55 1 291 405 3 118 2.82e-33 121
MsG0680034921.01.T01 MTR_1g115205 34.733 262 126 9 1 258 17 237 3.06e-31 120
MsG0680034921.01.T01 MTR_8g073570 42.778 180 81 7 15 189 8 170 3.25e-29 112
MsG0680034921.01.T01 MTR_5g037190 34.848 264 130 10 1 257 1 229 1.32e-27 110
MsG0680034921.01.T01 MTR_7g017150 42.188 192 99 6 216 404 82 264 2.89e-27 109
MsG0680034921.01.T01 MTR_5g097540 43.478 161 78 4 205 365 86 233 3.07e-27 110
MsG0680034921.01.T01 MTR_5g036250 60.976 82 31 1 318 399 23 103 7.91e-27 106
MsG0680034921.01.T01 MTR_7g017120 41.667 192 100 6 216 404 81 263 1.00e-26 108
MsG0680034921.01.T01 MTR_5g086780 50.000 106 53 0 278 383 123 228 7.70e-26 104
MsG0680034921.01.T01 MTR_5g086780 64.407 59 20 1 1 59 1 58 8.12e-14 70.9
MsG0680034921.01.T01 MTR_2g104260 33.333 297 153 12 7 282 4 276 1.74e-25 105
MsG0680034921.01.T01 MTR_2g022020 50.000 102 49 1 302 403 17 116 2.49e-25 99.8
MsG0680034921.01.T01 MTR_4g133170 24.863 366 225 12 20 377 7 330 3.70e-23 100
MsG0680034921.01.T01 MTR_5g097650 41.259 143 72 5 12 152 17 149 3.99e-23 95.1
MsG0680034921.01.T01 MTR_6g087660 63.750 80 28 1 1 79 1 80 8.41e-23 95.5
MsG0680034921.01.T01 MTR_3g019290 37.297 185 72 6 14 198 11 151 5.22e-22 92.8
MsG0680034921.01.T01 MTR_7g101200 31.120 241 113 9 131 364 34 228 2.22e-21 92.4
MsG0680034921.01.T01 MTR_2g426070 25.193 389 213 19 25 380 14 357 6.76e-21 94.0
MsG0680034921.01.T01 MTR_1g047080 27.324 355 177 14 54 403 1 279 5.48e-20 89.7
MsG0680034921.01.T01 MTR_2g026010 24.044 366 214 17 36 382 23 343 3.59e-19 89.0
MsG0680034921.01.T01 MTR_8g041440 45.763 118 37 4 6 114 3 102 4.02e-19 82.8
MsG0680034921.01.T01 MTR_1g068875 22.933 375 228 16 20 380 7 334 4.23e-19 88.6
MsG0680034921.01.T01 MTR_5g096070 40.000 145 55 4 13 153 22 138 1.14e-18 82.8
MsG0680034921.01.T01 MTR_2g425980 23.664 393 222 18 20 379 8 355 1.94e-17 84.0
MsG0680034921.01.T01 MTR_2g028110 24.599 374 226 16 20 380 7 337 2.36e-17 83.6
MsG0680034921.01.T01 MTR_5g091150 34.518 197 75 8 117 312 40 183 3.43e-17 80.1
MsG0680034921.01.T01 MTR_5g096050 47.619 105 45 3 250 345 1 104 4.22e-17 77.4
MsG0680034921.01.T01 MTR_2g031720 24.802 379 223 20 20 380 9 343 1.11e-16 81.6
MsG0680034921.01.T01 MTR_1g068860 24.603 378 221 17 20 380 7 337 1.37e-16 81.3
MsG0680034921.01.T01 MTR_1g068835 24.869 382 213 19 20 380 7 335 2.34e-16 80.5
MsG0680034921.01.T01 MTR_3g047580 39.007 141 63 6 11 149 8 127 5.48e-16 74.3
MsG0680034921.01.T01 MTR_2g025420 23.116 398 235 17 20 380 9 372 7.94e-16 79.3
MsG0680034921.01.T01 MTR_2g021050 23.670 376 219 15 20 380 8 330 1.25e-15 78.2
MsG0680034921.01.T01 MTR_2g031640 24.129 373 223 16 20 377 9 336 1.50e-15 78.2
MsG0680034921.01.T01 MTR_2g426060 25.065 387 223 21 20 379 9 355 2.76e-15 77.4
MsG0680034921.01.T01 MTR_3g007410 25.656 343 188 13 25 349 60 353 3.90e-15 77.0
MsG0680034921.01.T01 MTR_2g028130 23.810 399 226 18 20 380 8 366 6.78e-15 76.3
MsG0680034921.01.T01 MTR_3g007360 25.882 340 191 14 25 349 60 353 7.25e-15 76.3
MsG0680034921.01.T01 MTR_3g013770 23.037 382 248 13 20 397 36 375 1.31e-14 75.5
MsG0680034921.01.T01 MTR_4g070550 23.544 395 228 18 20 381 8 361 1.69e-14 75.1
MsG0680034921.01.T01 MTR_3g097190 54.717 53 24 0 327 379 28 80 3.04e-14 70.9
MsG0680034921.01.T01 MTR_2g028160 22.980 396 235 16 20 380 8 368 5.22e-14 73.6
MsG0680034921.01.T01 MTR_2g024100 23.077 377 227 14 31 380 21 361 5.67e-14 73.6
MsG0680034921.01.T01 MTR_4g116330 22.549 408 268 12 2 403 8 373 5.97e-14 73.2
MsG0680034921.01.T01 MTR_1g050305 48.837 86 39 2 310 390 23 108 9.86e-14 68.6
MsG0680034921.01.T01 MTR_2g031680 25.199 377 227 18 12 377 1 333 1.88e-13 71.6
MsG0680034921.01.T01 MTR_8g099460 24.031 387 220 16 20 380 8 346 2.65e-13 71.2
MsG0680034921.01.T01 MTR_0064s0160 21.182 406 279 11 16 405 41 421 6.51e-13 70.5
MsG0680034921.01.T01 MTR_4g117850 23.192 401 238 14 20 403 26 373 7.62e-13 69.7
MsG0680034921.01.T01 MTR_4g117780 23.005 426 239 17 1 403 1 360 2.57e-12 68.2
MsG0680034921.01.T01 MTR_8g090195 53.571 56 26 0 326 381 58 113 2.67e-12 63.5
MsG0680034921.01.T01 MTR_4g117770 23.389 419 246 16 1 403 1 360 3.35e-12 67.8
MsG0680034921.01.T01 MTR_4g117810 23.389 419 246 16 1 403 1 360 3.35e-12 67.8
MsG0680034921.01.T01 MTR_4g021785 22.475 396 244 15 16 403 8 348 7.11e-12 66.6
MsG0680034921.01.T01 MTR_2g021150 22.941 340 209 12 16 346 13 308 2.62e-11 65.1
MsG0680034921.01.T01 MTR_3g007430 23.410 346 193 14 25 354 51 340 2.86e-11 65.1
MsG0680034921.01.T01 MTR_3g047540 47.826 92 33 5 216 306 6 83 4.97e-11 59.3
MsG0680034921.01.T01 MTR_2g046830 21.958 337 201 13 16 342 24 308 6.27e-11 63.9
MsG0680034921.01.T01 MTR_3g024130 25.784 287 167 14 67 345 5 253 9.48e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034921.01.T01 AT4G12560 23.509 285 175 10 120 385 92 352 1.33e-12 69.3
MsG0680034921.01.T01 AT4G12560 23.509 285 175 10 120 385 92 352 1.33e-12 69.3
MsG0680034921.01.T01 AT4G12560 23.509 285 175 10 120 385 92 352 1.33e-12 69.3

Find 76 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGGTGACTTATACAATAT+TGG 0.205348 6:+96635849 MsG0680034921.01.T01:CDS
GATTGCGGAGCTTGGTTATA+AGG 0.254764 6:+96636367 MsG0680034921.01.T01:CDS
TAAACTTGGCGGCGTTAAAT+AGG 0.265797 6:+96636135 MsG0680034921.01.T01:CDS
TTAACTACTTATTCCTCTAA+TGG 0.319115 6:-96636437 None:intergenic
TCATCTTCGTCCGAGGAAAT+AGG 0.322393 6:-96636086 None:intergenic
CTCTCTGTGATATGAGTATA+AGG 0.329557 6:-96636165 None:intergenic
CATCACTTCATGACCATGTT+TGG 0.338862 6:+96635384 MsG0680034921.01.T01:CDS
AAAATAGAATAAGTCATATA+AGG 0.350379 6:-96635933 None:intergenic
TTTGAAGACAACGACGATAT+TGG 0.352551 6:+96635558 MsG0680034921.01.T01:CDS
GATGTTAAGCCACGTATTCA+TGG 0.357284 6:+96635747 MsG0680034921.01.T01:CDS
TAGCCCCGCCGGACCATGTT+TGG 0.379833 6:+96636025 MsG0680034921.01.T01:CDS
CTGGTGACTTATACAATATT+GGG 0.379859 6:+96635850 MsG0680034921.01.T01:CDS
ATTCTTCATAAGAAAAGATA+TGG 0.381516 6:+96636313 MsG0680034921.01.T01:CDS
GAGGAACGGACTTGATTGTT+TGG 0.387612 6:-96635662 None:intergenic
GGAAAGCATTCTTCCATTAG+AGG 0.399007 6:+96636424 MsG0680034921.01.T01:CDS
AAACGCTTAAAAGATTTAAG+AGG 0.406317 6:-96635321 None:intergenic
AAGCCACGTATTCATGGATT+CGG 0.415201 6:+96635753 MsG0680034921.01.T01:CDS
CCTTATCACACCTATTTCCT+CGG 0.438410 6:+96636076 MsG0680034921.01.T01:CDS
AGGAACGGACTTGATTGTTT+GGG 0.449009 6:-96635661 None:intergenic
CTCAGCCAACTCAACCTCAC+TGG 0.455295 6:-96635685 None:intergenic
GACACCAAACATGGTCCGGC+GGG 0.457799 6:-96636029 None:intergenic
TTCTCCACGAGTCACTTCTT+AGG 0.461891 6:-96635893 None:intergenic
TTGAATCTGCTCGCAAATCA+TGG 0.463031 6:+96635343 MsG0680034921.01.T01:CDS
AGGCAAAGAGTCTTGGTTGT+AGG 0.463721 6:+96636387 MsG0680034921.01.T01:CDS
GAAAAGATATGGAAGTAGCT+TGG 0.464411 6:+96636324 MsG0680034921.01.T01:CDS
TCCCATTGTTCTTGTTATGA+CGG 0.472622 6:+96635429 MsG0680034921.01.T01:CDS
ACCGAACTGATTGCGGAGCT+TGG 0.473192 6:+96636359 MsG0680034921.01.T01:CDS
TCCTCCCAGTGAGGTTGAGT+TGG 0.479745 6:+96635680 MsG0680034921.01.T01:CDS
CTCGGTTCCACAATACAATA+TGG 0.481371 6:-96635635 None:intergenic
AAGAAAATCAATATCTTCAT+CGG 0.482543 6:-96635718 None:intergenic
AACGTTTCTATAATGTCATC+GGG 0.491695 6:+96635819 MsG0680034921.01.T01:CDS
TAAATGACTACAAGATCATT+CGG 0.493903 6:+96635790 MsG0680034921.01.T01:CDS
GGTTATAAGGCAAAGAGTCT+TGG 0.494956 6:+96636380 MsG0680034921.01.T01:CDS
ACTTGATTGTTTGGGTAGCT+CGG 0.495006 6:-96635653 None:intergenic
ACCAAGCTCCGCAATCAGTT+CGG 0.502016 6:-96636360 None:intergenic
CCGAGGAAATAGGTGTGATA+AGG 0.507291 6:-96636076 None:intergenic
AAACGTTTCTATAATGTCAT+CGG 0.513054 6:+96635818 MsG0680034921.01.T01:CDS
AAATGACACCAAACATGGTC+CGG 0.516116 6:-96636033 None:intergenic
CTCAACCTCACTGGGAGGAA+CGG 0.516387 6:-96635676 None:intergenic
TCTATTCTCTTGCTGGTGAG+AGG 0.516733 6:+96635499 MsG0680034921.01.T01:CDS
ACATCGATGGAGTATGTCAT+TGG 0.518452 6:+96635979 MsG0680034921.01.T01:CDS
ATGTCATCGGGTAAATACTC+TGG 0.521921 6:+96635831 MsG0680034921.01.T01:CDS
TCCGTCATAACAAGAACAAT+GGG 0.526151 6:-96635430 None:intergenic
CAATATGGTCATATACATCA+TGG 0.537311 6:-96635620 None:intergenic
GTTAAACGTCCTATCGGAGT+GGG 0.538293 6:+96636275 MsG0680034921.01.T01:CDS
TATACAATATTGGGCCAACA+TGG 0.546223 6:+96635859 MsG0680034921.01.T01:CDS
AGGCTATATATCTCCCATGT+TGG 0.548860 6:-96635873 None:intergenic
ATACTCCATCGATGTAGACT+TGG 0.552081 6:-96635971 None:intergenic
CTCCGTCATAACAAGAACAA+TGG 0.553989 6:-96635431 None:intergenic
TAAGAAATTGCTGCCAAACA+TGG 0.554116 6:-96635397 None:intergenic
TCAGCCAACTCAACCTCACT+GGG 0.563772 6:-96635684 None:intergenic
TTGTAGGATAGTAAATTACA+AGG 0.567320 6:+96636403 MsG0680034921.01.T01:CDS
CCTTGCGTTAAACGTCCTAT+CGG 0.575779 6:+96636269 MsG0680034921.01.T01:CDS
GGCACCCAAGTCTACATCGA+TGG 0.576959 6:+96635966 MsG0680034921.01.T01:CDS
GTGATATGAGTATAAGGCAA+TGG 0.580197 6:-96636159 None:intergenic
TTTGAGTACCGAACTGATTG+CGG 0.595965 6:+96636352 MsG0680034921.01.T01:CDS
GGACGTTTAACGCAAGGCAA+CGG 0.600723 6:-96636263 None:intergenic
TACTCCATCGATGTAGACTT+GGG 0.605400 6:-96635970 None:intergenic
TATATGACCATATTGTATTG+TGG 0.619962 6:+96635628 MsG0680034921.01.T01:CDS
CGTTAAACGTCCTATCGGAG+TGG 0.621791 6:+96636274 MsG0680034921.01.T01:CDS
AAAACAATCATCTTCGTCCG+AGG 0.622353 6:-96636093 None:intergenic
AGCTCGGTCTAAAAGAATCG+TGG 0.626081 6:+96636219 MsG0680034921.01.T01:CDS
CCGATAGGACGTTTAACGCA+AGG 0.627066 6:-96636269 None:intergenic
AAGTAAAATGACACCAAACA+TGG 0.627920 6:-96636038 None:intergenic
ATACAATATTGGGCCAACAT+GGG 0.628326 6:+96635860 MsG0680034921.01.T01:CDS
TGACACCAAACATGGTCCGG+CGG 0.631611 6:-96636030 None:intergenic
ATAGCCTAAGAAGTGACTCG+TGG 0.654513 6:+96635889 MsG0680034921.01.T01:CDS
ACACCAAACATGGTCCGGCG+GGG 0.658473 6:-96636028 None:intergenic
TAAACGTCCTATCGGAGTGG+GGG 0.664853 6:+96636277 MsG0680034921.01.T01:CDS
TTAAACGTCCTATCGGAGTG+GGG 0.668734 6:+96636276 MsG0680034921.01.T01:CDS
AACAGTGGCTGTGATGAATG+AGG 0.673791 6:+96635245 MsG0680034921.01.T01:CDS
GCCAACTCAACCTCACTGGG+AGG 0.676738 6:-96635681 None:intergenic
TAACCGAATCCATGAATACG+TGG 0.693562 6:-96635756 None:intergenic
CAAGTCCGTTCCTCCCAGTG+AGG 0.695018 6:+96635671 MsG0680034921.01.T01:CDS
TTGGATGGAGAAGACAACAG+TGG 0.747342 6:+96635230 None:intergenic
AGTGGCTGTGATGAATGAGG+TGG 0.764254 6:+96635248 MsG0680034921.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAGAATAAGTCATATA+AGG - Chr6:96635936-96635955 None:intergenic 15.0%
!!! ACTTTTCATATATCAATTTT+GGG + Chr6:96636194-96636213 MsG0680034921.01.T01:CDS 15.0%
!!! TACTTTTCATATATCAATTT+TGG + Chr6:96636193-96636212 MsG0680034921.01.T01:CDS 15.0%
!! AAGAAAATCAATATCTTCAT+CGG - Chr6:96635721-96635740 None:intergenic 20.0%
!! ATTCTTCATAAGAAAAGATA+TGG + Chr6:96636313-96636332 MsG0680034921.01.T01:CDS 20.0%
!!! TATTCTATTTTGTATACTGA+TGG + Chr6:96635945-96635964 MsG0680034921.01.T01:CDS 20.0%
! AAACGCTTAAAAGATTTAAG+AGG - Chr6:96635324-96635343 None:intergenic 25.0%
! AAACGTTTCTATAATGTCAT+CGG + Chr6:96635818-96635837 MsG0680034921.01.T01:CDS 25.0%
! TAAATGACTACAAGATCATT+CGG + Chr6:96635790-96635809 MsG0680034921.01.T01:CDS 25.0%
! TATATGACCATATTGTATTG+TGG + Chr6:96635628-96635647 MsG0680034921.01.T01:CDS 25.0%
! TTGTAGGATAGTAAATTACA+AGG + Chr6:96636403-96636422 MsG0680034921.01.T01:CDS 25.0%
!!! GATATTGGTATTTTTGGTTT+TGG + Chr6:96635573-96635592 MsG0680034921.01.T01:CDS 25.0%
!!! GTTTTGATGTTGTAAAAAAG+CGG + Chr6:96636111-96636130 MsG0680034921.01.T01:CDS 25.0%
AACGTTTCTATAATGTCATC+GGG + Chr6:96635819-96635838 MsG0680034921.01.T01:CDS 30.0%
AAGTAAAATGACACCAAACA+TGG - Chr6:96636041-96636060 None:intergenic 30.0%
CAATATGGTCATATACATCA+TGG - Chr6:96635623-96635642 None:intergenic 30.0%
CTGGTGACTTATACAATATT+GGG + Chr6:96635850-96635869 MsG0680034921.01.T01:CDS 30.0%
TCTGGTGACTTATACAATAT+TGG + Chr6:96635849-96635868 MsG0680034921.01.T01:CDS 30.0%
TGTAAAAAAGCGGATAAACT+TGG + Chr6:96636121-96636140 MsG0680034921.01.T01:CDS 30.0%
! AACGACGATATTGGTATTTT+TGG + Chr6:96635567-96635586 MsG0680034921.01.T01:CDS 30.0%
! AGTGATGATTTTCAAACAAG+AGG - Chr6:96635371-96635390 None:intergenic 30.0%
! GTGATGATTTTCAAACAAGA+GGG - Chr6:96635370-96635389 None:intergenic 30.0%
!!! TTTGGTTTTGGTAGTATTAG+TGG + Chr6:96635585-96635604 MsG0680034921.01.T01:CDS 30.0%
AAAAAAGCGGATAAACTTGG+CGG + Chr6:96636124-96636143 MsG0680034921.01.T01:CDS 35.0%
ATACAATATTGGGCCAACAT+GGG + Chr6:96635860-96635879 MsG0680034921.01.T01:CDS 35.0%
CTCTCTGTGATATGAGTATA+AGG - Chr6:96636168-96636187 None:intergenic 35.0%
GAAAAGATATGGAAGTAGCT+TGG + Chr6:96636324-96636343 MsG0680034921.01.T01:CDS 35.0%
GTGATATGAGTATAAGGCAA+TGG - Chr6:96636162-96636181 None:intergenic 35.0%
TAAGAAATTGCTGCCAAACA+TGG - Chr6:96635400-96635419 None:intergenic 35.0%
TATACAATATTGGGCCAACA+TGG + Chr6:96635859-96635878 MsG0680034921.01.T01:CDS 35.0%
TCCGTCATAACAAGAACAAT+GGG - Chr6:96635433-96635452 None:intergenic 35.0%
TTTGAAGACAACGACGATAT+TGG + Chr6:96635558-96635577 MsG0680034921.01.T01:CDS 35.0%
! GATGTTTTCTATTCTCTTGC+TGG + Chr6:96635492-96635511 MsG0680034921.01.T01:CDS 35.0%
! TCCCATTGTTCTTGTTATGA+CGG + Chr6:96635429-96635448 MsG0680034921.01.T01:CDS 35.0%
!! ATATCAATTTTGGGTGAGCT+CGG + Chr6:96636203-96636222 MsG0680034921.01.T01:CDS 35.0%
!! CAGAGTATTTGCACTCTTTT+AGG - Chr6:96635467-96635486 None:intergenic 35.0%
!! GTCATTTTACTTGAGCAATG+AGG + Chr6:96636049-96636068 MsG0680034921.01.T01:CDS 35.0%
!! TGGACCAAAGTTTTTGTTGT+TGG + Chr6:96636239-96636258 MsG0680034921.01.T01:CDS 35.0%
AAAACAATCATCTTCGTCCG+AGG - Chr6:96636096-96636115 None:intergenic 40.0%
AAATGACACCAAACATGGTC+CGG - Chr6:96636036-96636055 None:intergenic 40.0%
AAGCCACGTATTCATGGATT+CGG + Chr6:96635753-96635772 MsG0680034921.01.T01:CDS 40.0%
ACATCGATGGAGTATGTCAT+TGG + Chr6:96635979-96635998 MsG0680034921.01.T01:CDS 40.0%
ACTTGATTGTTTGGGTAGCT+CGG - Chr6:96635656-96635675 None:intergenic 40.0%
AGGCTATATATCTCCCATGT+TGG - Chr6:96635876-96635895 None:intergenic 40.0%
ATACTCCATCGATGTAGACT+TGG - Chr6:96635974-96635993 None:intergenic 40.0%
ATGTCATCGGGTAAATACTC+TGG + Chr6:96635831-96635850 MsG0680034921.01.T01:CDS 40.0%
CATCACTTCATGACCATGTT+TGG + Chr6:96635384-96635403 MsG0680034921.01.T01:CDS 40.0%
CCTTATCACACCTATTTCCT+CGG + Chr6:96636076-96636095 MsG0680034921.01.T01:CDS 40.0%
CGGTCCAACAACAAAAACTT+TGG - Chr6:96636246-96636265 None:intergenic 40.0%
CTCCGTCATAACAAGAACAA+TGG - Chr6:96635434-96635453 None:intergenic 40.0%
CTCGGTTCCACAATACAATA+TGG - Chr6:96635638-96635657 None:intergenic 40.0%
GATGTTAAGCCACGTATTCA+TGG + Chr6:96635747-96635766 MsG0680034921.01.T01:CDS 40.0%
GGTTATAAGGCAAAGAGTCT+TGG + Chr6:96636380-96636399 MsG0680034921.01.T01:CDS 40.0%
TAAACTTGGCGGCGTTAAAT+AGG + Chr6:96636135-96636154 MsG0680034921.01.T01:CDS 40.0%
TAACCGAATCCATGAATACG+TGG - Chr6:96635759-96635778 None:intergenic 40.0%
TACTCCATCGATGTAGACTT+GGG - Chr6:96635973-96635992 None:intergenic 40.0%
TTGAATCTGCTCGCAAATCA+TGG + Chr6:96635343-96635362 MsG0680034921.01.T01:CDS 40.0%
! AGGAACGGACTTGATTGTTT+GGG - Chr6:96635664-96635683 None:intergenic 40.0%
!! GGAAAGCATTCTTCCATTAG+AGG + Chr6:96636424-96636443 MsG0680034921.01.T01:CDS 40.0%
!! TTTGAGTACCGAACTGATTG+CGG + Chr6:96636352-96636371 MsG0680034921.01.T01:CDS 40.0%
AACAGTGGCTGTGATGAATG+AGG + Chr6:96635245-96635264 MsG0680034921.01.T01:CDS 45.0%
AGCTCGGTCTAAAAGAATCG+TGG + Chr6:96636219-96636238 MsG0680034921.01.T01:CDS 45.0%
AGGCAAAGAGTCTTGGTTGT+AGG + Chr6:96636387-96636406 MsG0680034921.01.T01:CDS 45.0%
ATAGCCTAAGAAGTGACTCG+TGG + Chr6:96635889-96635908 MsG0680034921.01.T01:CDS 45.0%
CCGAGGAAATAGGTGTGATA+AGG - Chr6:96636079-96636098 None:intergenic 45.0%
CCTTGCGTTAAACGTCCTAT+CGG + Chr6:96636269-96636288 MsG0680034921.01.T01:CDS 45.0%
GATTGCGGAGCTTGGTTATA+AGG + Chr6:96636367-96636386 MsG0680034921.01.T01:CDS 45.0%
GTTAAACGTCCTATCGGAGT+GGG + Chr6:96636275-96636294 MsG0680034921.01.T01:CDS 45.0%
TCATCTTCGTCCGAGGAAAT+AGG - Chr6:96636089-96636108 None:intergenic 45.0%
TCTATTCTCTTGCTGGTGAG+AGG + Chr6:96635499-96635518 MsG0680034921.01.T01:CDS 45.0%
TTAAACGTCCTATCGGAGTG+GGG + Chr6:96636276-96636295 MsG0680034921.01.T01:CDS 45.0%
TTCTCCACGAGTCACTTCTT+AGG - Chr6:96635896-96635915 None:intergenic 45.0%
! GAGGAACGGACTTGATTGTT+TGG - Chr6:96635665-96635684 None:intergenic 45.0%
ACCAAGCTCCGCAATCAGTT+CGG - Chr6:96636363-96636382 None:intergenic 50.0%
AGTGGCTGTGATGAATGAGG+TGG + Chr6:96635248-96635267 MsG0680034921.01.T01:CDS 50.0%
CCGATAGGACGTTTAACGCA+AGG - Chr6:96636272-96636291 None:intergenic 50.0%
CGTTAAACGTCCTATCGGAG+TGG + Chr6:96636274-96636293 MsG0680034921.01.T01:CDS 50.0%
GATGAAAAACATAGCCCCGC+CGG + Chr6:96636014-96636033 MsG0680034921.01.T01:CDS 50.0%
GGACGTTTAACGCAAGGCAA+CGG - Chr6:96636266-96636285 None:intergenic 50.0%
TAAACGTCCTATCGGAGTGG+GGG + Chr6:96636277-96636296 MsG0680034921.01.T01:CDS 50.0%
TCAGCCAACTCAACCTCACT+GGG - Chr6:96635687-96635706 None:intergenic 50.0%
ACCGAACTGATTGCGGAGCT+TGG + Chr6:96636359-96636378 MsG0680034921.01.T01:CDS 55.0%
CCTATCGGAGTGGGGGTAAA+GGG + Chr6:96636284-96636303 MsG0680034921.01.T01:CDS 55.0%
CTATCGGAGTGGGGGTAAAG+GGG + Chr6:96636285-96636304 MsG0680034921.01.T01:CDS 55.0%
CTCAACCTCACTGGGAGGAA+CGG - Chr6:96635679-96635698 None:intergenic 55.0%
CTCAGCCAACTCAACCTCAC+TGG - Chr6:96635688-96635707 None:intergenic 55.0%
GGCACCCAAGTCTACATCGA+TGG + Chr6:96635966-96635985 MsG0680034921.01.T01:CDS 55.0%
TATCGGAGTGGGGGTAAAGG+GGG + Chr6:96636286-96636305 MsG0680034921.01.T01:CDS 55.0%
TCCTATCGGAGTGGGGGTAA+AGG + Chr6:96636283-96636302 MsG0680034921.01.T01:CDS 55.0%
TCCTCCCAGTGAGGTTGAGT+TGG + Chr6:96635680-96635699 MsG0680034921.01.T01:CDS 55.0%
TGACACCAAACATGGTCCGG+CGG - Chr6:96636033-96636052 None:intergenic 55.0%
ACACCAAACATGGTCCGGCG+GGG - Chr6:96636031-96636050 None:intergenic 60.0%
CAAGTCCGTTCCTCCCAGTG+AGG + Chr6:96635671-96635690 MsG0680034921.01.T01:CDS 60.0%
CCCTTTACCCCCACTCCGAT+AGG - Chr6:96636287-96636306 None:intergenic 60.0%
GACACCAAACATGGTCCGGC+GGG - Chr6:96636032-96636051 None:intergenic 60.0%
GCCAACTCAACCTCACTGGG+AGG - Chr6:96635684-96635703 None:intergenic 60.0%
TAGCCCCGCCGGACCATGTT+TGG + Chr6:96636025-96636044 MsG0680034921.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr6 gene 96635234 96636451 96635234 ID=MsG0680034921.01;Name=MsG0680034921.01
Chr6 mRNA 96635234 96636451 96635234 ID=MsG0680034921.01.T01;Parent=MsG0680034921.01;Name=MsG0680034921.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|405
Chr6 exon 96635234 96636451 96635234 ID=MsG0680034921.01.T01:exon:6963;Parent=MsG0680034921.01.T01
Chr6 CDS 96635234 96636451 96635234 ID=MsG0680034921.01.T01:cds;Parent=MsG0680034921.01.T01
Gene Sequence

>MsG0680034921.01.T01

ATGGAGAAGACAACAGTGGCTGTGATGAATGAGGTGGTGAGTACATATGTATGTGATGATATAGCTTTTTCAATTCTCTCAAAGTTGCCTCTTAAATCTTTTAAGCGTTTTGAATCTGCTCGCAAATCATGGTCCCTCTTGTTTGAAAATCATCACTTCATGACCATGTTTGGCAGCAATTTCTTATCTAATTCTTCCCATTGTTCTTGTTATGACGGAGCATCTCTAATCCTAAAAGAGTGCAAATACTCTGAAGATGATGTTTTCTATTCTCTTGCTGGTGAGAGGTTTGAGAAAAAAGTCAAATTAGATTTCTCAAATCTATTTGAAGACAACGACGATATTGGTATTTTTGGTTTTGGTAGTATTAGTGGCATGCTTTGTGTCCATGATGTATATGACCATATTGTATTGTGGAACCGAGCTACCCAAACAATCAAGTCCGTTCCTCCCAGTGAGGTTGAGTTGGCTGAGTCGTTTATCACCGATGAAGATATTGATTTTCTTGAAATTGATGTTAAGCCACGTATTCATGGATTCGGTTACGATCTTGTTATAAATGACTACAAGATCATTCGGCATACAAACGTTTCTATAATGTCATCGGGTAAATACTCTGGTGACTTATACAATATTGGGCCAACATGGGAGATATATAGCCTAAGAAGTGACTCGTGGAGAAAGCTTTGTGTTGACATGCCTTATATGACTTATTCTATTTTGTATACTGATGGCACCCAAGTCTACATCGATGGAGTATGTCATTGGTTGTGTAAAAAAGATGAAAAACATAGCCCCGCCGGACCATGTTTGGTGTCATTTTACTTGAGCAATGAGGTGTTCCTTATCACACCTATTTCCTCGGACGAAGATGATTGTTTTGATGTTGTAAAAAAGCGGATAAACTTGGCGGCGTTAAATAGGTCCATTGCCTTATACTCATATCACAGAGAGACAACTACTTTTCATATATCAATTTTGGGTGAGCTCGGTCTAAAAGAATCGTGGACCAAAGTTTTTGTTGTTGGACCGTTGCCTTGCGTTAAACGTCCTATCGGAGTGGGGGTAAAGGGGGAAATATTCTTCATAAGAAAAGATATGGAAGTAGCTTGGTTTGATTTGAGTACCGAACTGATTGCGGAGCTTGGTTATAAGGCAAAGAGTCTTGGTTGTAGGATAGTAAATTACAAGGAAAGCATTCTTCCATTAGAGGAATAA
Protein sequence

>MsG0680034921.01.T01

MEKTTVAVMNEVVSTYVCDDIAFSILSKLPLKSFKRFESARKSWSLLFENHHFMTMFGSNFLSNSSHCSCYDGASLILKECKYSEDDVFYSLAGERFEKKVKLDFSNLFEDNDDIGIFGFGSISGMLCVHDVYDHIVLWNRATQTIKSVPPSEVELAESFITDEDIDFLEIDVKPRIHGFGYDLVINDYKIIRHTNVSIMSSGKYSGDLYNIGPTWEIYSLRSDSWRKLCVDMPYMTYSILYTDGTQVYIDGVCHWLCKKDEKHSPAGPCLVSFYLSNEVFLITPISSDEDDCFDVVKKRINLAALNRSIALYSYHRETTTFHISILGELGLKESWTKVFVVGPLPCVKRPIGVGVKGEIFFIRKDMEVAWFDLSTELIAELGYKAKSLGCRIVNYKESILPLEE*