AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680034882.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034882.01.T01 MTR_6g080680 73.927 303 73 5 3 301 89 389 8.47e-152 431
MsG0680034882.01.T01 MTR_4g028510 60.372 323 105 13 3 307 85 402 1.72e-115 341
MsG0680034882.01.T01 MTR_6g079810 56.881 327 112 7 6 305 137 461 3.58e-112 333
MsG0680034882.01.T01 MTR_6g079770 54.819 332 120 6 5 308 89 418 9.77e-109 322
MsG0680034882.01.T01 MTR_4g028660 57.372 312 114 10 3 299 84 391 3.69e-108 320
MsG0680034882.01.T01 MTR_4g027450 57.372 312 114 10 3 299 84 391 3.69e-108 320
MsG0680034882.01.T01 MTR_0134s0060 54.054 333 123 7 4 308 83 413 1.37e-107 319
MsG0680034882.01.T01 MTR_4g028680 56.966 323 117 10 3 308 84 401 1.98e-107 318
MsG0680034882.01.T01 MTR_0054s0100 53.988 326 128 8 3 308 86 409 7.17e-107 317
MsG0680034882.01.T01 MTR_0054s0090 54.294 326 125 8 3 308 86 407 1.17e-105 314
MsG0680034882.01.T01 MTR_6g078010 56.352 307 113 10 5 302 36 330 2.02e-104 309
MsG0680034882.01.T01 MTR_4g130330 56.308 325 117 13 3 307 72 391 1.26e-103 308
MsG0680034882.01.T01 MTR_6g090250 52.035 344 115 10 5 303 82 420 4.51e-102 305
MsG0680034882.01.T01 MTR_6g086825 52.410 332 126 9 2 308 137 461 3.60e-100 302
MsG0680034882.01.T01 MTR_6g090180 52.648 321 116 10 9 302 122 433 6.62e-97 293
MsG0680034882.01.T01 MTR_4g028750 53.821 301 119 8 3 288 84 379 6.68e-96 289
MsG0680034882.01.T01 MTR_6g079790 52.532 316 118 9 18 308 2 310 2.13e-92 277
MsG0680034882.01.T01 MTR_7g023800 51.000 300 115 5 3 298 31 302 1.21e-88 267
MsG0680034882.01.T01 MTR_6g478230 48.936 329 133 11 2 308 93 408 4.11e-87 267
MsG0680034882.01.T01 MTR_6g087620 51.325 302 113 8 6 297 2 279 2.00e-86 261
MsG0680034882.01.T01 MTR_0129s0070 46.154 325 139 10 5 308 88 397 2.17e-79 247
MsG0680034882.01.T01 MTR_8g063440 46.688 317 135 9 8 308 86 384 2.23e-79 246
MsG0680034882.01.T01 MTR_6g090260 61.000 200 71 4 110 303 62 260 5.51e-76 233
MsG0680034882.01.T01 MTR_0129s0120 45.031 322 143 9 5 306 88 395 9.67e-75 234
MsG0680034882.01.T01 MTR_5g069390 43.344 323 145 10 10 307 108 417 4.49e-72 231
MsG0680034882.01.T01 MTR_5g097220 42.941 340 150 10 3 307 78 408 5.47e-72 228
MsG0680034882.01.T01 MTR_2g038680 44.248 339 133 13 5 306 91 410 2.64e-70 224
MsG0680034882.01.T01 MTR_5g097680 42.815 341 149 11 9 308 93 428 6.94e-70 223
MsG0680034882.01.T01 MTR_5g037680 44.231 312 136 11 9 301 88 380 3.36e-69 222
MsG0680034882.01.T01 MTR_5g059430 43.692 325 141 11 10 305 113 424 1.08e-68 220
MsG0680034882.01.T01 MTR_2g022000 42.857 329 141 11 1 297 91 404 1.43e-68 220
MsG0680034882.01.T01 MTR_2g021770 40.854 328 154 9 9 308 93 408 3.65e-68 218
MsG0680034882.01.T01 MTR_5g092850 41.818 330 148 10 4 302 81 397 5.70e-68 218
MsG0680034882.01.T01 MTR_2g021800 43.072 332 141 13 10 308 101 417 1.44e-67 217
MsG0680034882.01.T01 MTR_8g064890 42.945 326 145 13 4 308 84 389 1.81e-67 216
MsG0680034882.01.T01 MTR_4g007940 40.000 335 152 11 4 308 27 342 2.02e-67 214
MsG0680034882.01.T01 MTR_5g064700 41.791 335 155 11 9 307 38 368 8.01e-66 211
MsG0680034882.01.T01 MTR_3g011120 43.234 303 138 12 8 296 87 369 1.24e-65 210
MsG0680034882.01.T01 MTR_5g097330 43.807 331 143 13 5 308 90 404 1.47e-65 211
MsG0680034882.01.T01 MTR_8g467670 42.633 319 145 12 5 308 85 380 5.06e-65 209
MsG0680034882.01.T01 MTR_0831s0010 40.439 319 144 10 9 306 127 420 2.09e-64 209
MsG0680034882.01.T01 MTR_4g102410 42.202 327 151 13 4 305 135 448 1.52e-63 207
MsG0680034882.01.T01 MTR_7g017130 40.498 321 154 10 9 308 94 398 6.11e-63 204
MsG0680034882.01.T01 MTR_8g012090 43.793 290 120 8 1 276 74 334 1.11e-62 202
MsG0680034882.01.T01 MTR_5g096630 40.533 338 153 14 4 307 91 414 1.20e-62 204
MsG0680034882.01.T01 MTR_8g464770 42.208 308 147 10 6 301 86 374 1.39e-62 202
MsG0680034882.01.T01 MTR_2g066200 39.450 327 157 10 4 308 82 389 2.47e-62 202
MsG0680034882.01.T01 MTR_2g034700 42.904 303 139 10 9 299 88 368 2.51e-62 202
MsG0680034882.01.T01 MTR_0129s0050 40.774 336 150 10 8 307 86 408 2.70e-62 203
MsG0680034882.01.T01 MTR_5g043605 40.728 302 142 11 16 307 65 339 2.98e-62 202
MsG0680034882.01.T01 MTR_2g021950 43.438 320 136 13 9 302 92 392 4.02e-62 202
MsG0680034882.01.T01 MTR_5g097350 42.434 304 138 11 9 299 100 379 8.17e-62 201
MsG0680034882.01.T01 MTR_5g096910 41.824 318 146 12 10 306 93 392 2.39e-61 200
MsG0680034882.01.T01 MTR_2g066170 40.125 319 157 8 8 308 85 387 5.17e-61 199
MsG0680034882.01.T01 MTR_2g066150 43.730 311 136 12 8 308 80 361 1.30e-60 197
MsG0680034882.01.T01 MTR_6g461660 42.025 326 148 12 9 308 91 401 1.79e-60 198
MsG0680034882.01.T01 MTR_2g065840 41.358 324 155 13 3 306 80 388 1.22e-59 196
MsG0680034882.01.T01 MTR_7g017170 39.039 333 154 11 9 308 94 410 1.67e-59 196
MsG0680034882.01.T01 MTR_4g117550 40.994 322 129 11 8 308 93 374 2.68e-59 194
MsG0680034882.01.T01 MTR_8g046310 40.063 317 150 11 2 303 83 374 9.33e-59 193
MsG0680034882.01.T01 MTR_5g074850 41.534 313 139 14 2 283 101 400 1.28e-58 202
MsG0680034882.01.T01 MTR_7g069550 39.763 337 147 14 2 308 86 396 1.58e-58 193
MsG0680034882.01.T01 MTR_7g111700 41.018 334 118 12 9 308 78 366 4.41e-58 191
MsG0680034882.01.T01 MTR_8g046200 36.813 364 144 13 2 303 79 418 3.43e-57 190
MsG0680034882.01.T01 MTR_6g079730 65.493 142 47 2 163 303 6 146 5.03e-57 181
MsG0680034882.01.T01 MTR_1g007450 41.270 315 139 13 8 293 85 382 5.09e-57 189
MsG0680034882.01.T01 MTR_5g074800 41.818 330 138 16 9 308 36 341 6.08e-57 187
MsG0680034882.01.T01 MTR_5g068490 44.565 276 118 12 38 308 33 278 6.62e-57 185
MsG0680034882.01.T01 MTR_8g033140 39.714 350 136 16 9 308 95 419 7.89e-57 189
MsG0680034882.01.T01 MTR_5g024260 41.883 308 140 12 8 289 94 388 8.20e-57 189
MsG0680034882.01.T01 MTR_4g014370 39.286 336 154 12 8 307 88 409 8.96e-57 189
MsG0680034882.01.T01 MTR_2g104340 40.000 335 157 11 3 308 78 397 1.05e-56 188
MsG0680034882.01.T01 MTR_5g075370 40.064 312 145 13 4 283 75 376 1.21e-56 195
MsG0680034882.01.T01 MTR_1g053510 38.348 339 152 9 8 308 90 409 1.26e-56 188
MsG0680034882.01.T01 MTR_2g065860 42.006 319 154 13 8 308 84 389 1.78e-56 187
MsG0680034882.01.T01 MTR_7g017040 40.798 326 150 10 9 307 89 398 3.71e-56 187
MsG0680034882.01.T01 MTR_7g104710 47.619 231 80 6 3 222 85 285 6.30e-56 184
MsG0680034882.01.T01 MTR_1g007410 40.248 323 140 12 8 299 98 398 1.32e-55 185
MsG0680034882.01.T01 MTR_5g076490 42.988 328 133 14 9 307 34 336 3.17e-55 182
MsG0680034882.01.T01 MTR_2g104280 38.209 335 160 10 8 308 85 406 6.39e-55 184
MsG0680034882.01.T01 MTR_4g055820 42.414 290 126 9 2 263 83 359 6.49e-55 182
MsG0680034882.01.T01 MTR_5g086590 43.210 243 111 7 46 279 7 231 8.00e-55 178
MsG0680034882.01.T01 MTR_8g039500 40.559 286 135 8 8 276 79 346 9.84e-55 181
MsG0680034882.01.T01 MTR_6g004450 45.148 237 105 6 86 306 41 268 7.88e-54 177
MsG0680034882.01.T01 MTR_8g033090 38.551 345 145 14 9 307 89 412 9.12e-54 181
MsG0680034882.01.T01 MTR_1g050312 40.705 312 126 12 3 296 14 284 2.25e-53 176
MsG0680034882.01.T01 MTR_5g092860 41.158 311 119 13 8 283 68 349 2.38e-53 178
MsG0680034882.01.T01 MTR_8g033110 37.536 349 152 13 6 307 86 415 1.16e-51 175
MsG0680034882.01.T01 MTR_2g021920 45.992 237 94 8 95 302 37 268 3.15e-51 170
MsG0680034882.01.T01 MTR_7g079640 41.869 289 131 14 8 283 88 352 4.42e-51 173
MsG0680034882.01.T01 MTR_5g023970 49.741 193 92 4 119 308 7 197 1.60e-50 166
MsG0680034882.01.T01 MTR_8g041270 40.000 340 151 17 5 308 85 407 3.29e-50 171
MsG0680034882.01.T01 MTR_8g046220 36.390 349 145 13 2 303 84 402 4.97e-50 171
MsG0680034882.01.T01 MTR_5g097850 52.688 186 77 6 128 306 63 244 4.99e-50 166
MsG0680034882.01.T01 MTR_3g018750 40.891 247 106 9 94 307 2 241 3.66e-49 164
MsG0680034882.01.T01 MTR_8g036970 36.395 294 123 9 1 286 66 303 1.99e-48 166
MsG0680034882.01.T01 MTR_1g007430 38.732 284 128 8 4 265 65 324 7.15e-48 163
MsG0680034882.01.T01 MTR_8g064790 36.452 310 139 10 4 308 82 338 4.29e-47 161
MsG0680034882.01.T01 MTR_8g032980 37.360 356 151 13 9 308 83 422 4.48e-47 164
MsG0680034882.01.T01 MTR_8g018110 36.283 339 152 12 6 307 86 397 6.99e-47 162
MsG0680034882.01.T01 MTR_5g022850 37.202 336 159 15 4 308 40 354 7.92e-47 161
MsG0680034882.01.T01 MTR_1g098180 38.710 279 118 10 52 308 120 367 2.15e-46 160
MsG0680034882.01.T01 MTR_5g091140 39.490 314 123 12 4 287 121 397 6.20e-46 160
MsG0680034882.01.T01 MTR_5g066420 51.678 149 66 2 154 296 1 149 1.72e-45 151
MsG0680034882.01.T01 MTR_2g064950 36.943 314 137 11 2 281 78 364 1.39e-44 156
MsG0680034882.01.T01 MTR_8g063350 36.218 312 166 14 3 298 43 337 2.04e-43 152
MsG0680034882.01.T01 MTR_4g016000 41.553 219 80 8 57 257 2 190 1.02e-42 146
MsG0680034882.01.T01 MTR_8g035690 38.028 284 113 11 65 306 40 302 1.09e-42 149
MsG0680034882.01.T01 MTR_5g072060 37.954 303 148 14 9 292 80 361 2.57e-42 154
MsG0680034882.01.T01 MTR_6g009350 44.388 196 81 5 95 270 8 195 4.22e-42 144
MsG0680034882.01.T01 MTR_4g014370 44.103 195 94 5 120 307 151 337 4.75e-42 148
MsG0680034882.01.T01 MTR_8g033000 48.980 196 81 6 123 307 176 363 4.20e-41 146
MsG0680034882.01.T01 MTR_8g013000 38.516 283 134 11 8 265 86 353 1.50e-40 146
MsG0680034882.01.T01 MTR_8g063370 35.065 308 166 12 8 298 93 383 2.97e-40 145
MsG0680034882.01.T01 MTR_3g030620 47.929 169 78 7 145 305 14 180 3.32e-39 136
MsG0680034882.01.T01 MTR_8g063330 34.767 279 137 10 8 262 91 348 3.85e-39 143
MsG0680034882.01.T01 MTR_8g063310 33.546 313 171 12 8 298 91 388 5.74e-39 142
MsG0680034882.01.T01 MTR_7g016930 41.224 245 110 9 12 238 76 304 2.69e-37 135
MsG0680034882.01.T01 MTR_7g019250 38.785 214 97 5 87 283 216 412 4.53e-36 135
MsG0680034882.01.T01 MTR_5g096920 47.403 154 75 4 154 305 1 150 1.27e-35 126
MsG0680034882.01.T01 MTR_6g070880 45.181 166 64 6 3 145 100 261 3.37e-34 125
MsG0680034882.01.T01 MTR_6g071420 45.181 166 64 6 3 145 100 261 3.37e-34 125
MsG0680034882.01.T01 MTR_5g096900 39.113 248 106 12 9 230 85 313 3.09e-33 124
MsG0680034882.01.T01 MTR_5g097660 54.545 110 48 2 196 303 7 116 2.19e-32 117
MsG0680034882.01.T01 MTR_5g097540 46.897 145 67 5 123 265 97 233 2.88e-31 119
MsG0680034882.01.T01 MTR_5g098950 38.393 224 87 7 54 265 90 274 1.31e-30 116
MsG0680034882.01.T01 MTR_4g094400 58.491 106 27 4 151 240 7 111 1.47e-30 112
MsG0680034882.01.T01 MTR_7g017150 42.188 192 100 6 123 308 82 268 2.67e-30 115
MsG0680034882.01.T01 MTR_7g017120 41.667 192 101 6 123 308 81 267 1.33e-29 114
MsG0680034882.01.T01 MTR_5g036250 54.286 105 46 2 204 307 8 111 1.90e-28 108
MsG0680034882.01.T01 MTR_2g022020 49.138 116 54 3 191 303 3 116 1.54e-27 103
MsG0680034882.01.T01 MTR_7g101200 37.168 226 96 11 51 264 37 228 8.57e-26 102
MsG0680034882.01.T01 MTR_5g094350 34.951 206 94 5 9 192 89 276 1.83e-25 103
MsG0680034882.01.T01 MTR_1g047080 30.982 326 144 14 1 308 22 284 3.58e-25 102
MsG0680034882.01.T01 MTR_5g097820 38.824 170 73 6 9 161 99 254 1.56e-24 100
MsG0680034882.01.T01 MTR_4g133170 26.255 259 166 9 31 277 85 330 2.12e-22 96.7
MsG0680034882.01.T01 MTR_5g091150 35.450 189 84 7 26 214 34 184 6.74e-22 91.7
MsG0680034882.01.T01 MTR_5g096050 51.376 109 39 3 154 249 1 108 6.87e-22 89.4
MsG0680034882.01.T01 MTR_5g086780 48.598 107 53 2 178 283 123 228 1.12e-21 91.7
MsG0680034882.01.T01 MTR_1g050308 50.442 113 48 4 201 308 239 348 1.16e-21 94.0
MsG0680034882.01.T01 MTR_1g050308 35.862 145 66 6 8 140 107 236 1.99e-15 76.3
MsG0680034882.01.T01 MTR_2g104260 34.634 205 93 6 8 183 85 277 1.28e-21 92.4
MsG0680034882.01.T01 MTR_6g465360 41.722 151 63 5 4 131 133 281 1.92e-21 92.4
MsG0680034882.01.T01 MTR_2g426060 28.205 273 167 13 39 294 109 369 5.15e-21 92.8
MsG0680034882.01.T01 MTR_1g068835 26.531 294 185 14 4 282 60 337 1.79e-20 90.9
MsG0680034882.01.T01 MTR_2g425980 27.344 256 163 11 39 279 108 355 1.80e-20 91.3
MsG0680034882.01.T01 MTR_2g426070 26.953 256 162 10 40 282 116 359 4.09e-20 90.1
MsG0680034882.01.T01 MTR_2g028140 25.704 284 187 12 40 308 118 392 4.42e-20 90.1
MsG0680034882.01.T01 MTR_2g028130 25.283 265 163 10 40 282 117 368 1.43e-19 88.6
MsG0680034882.01.T01 MTR_2g026010 26.449 276 162 15 31 282 85 343 1.86e-19 88.2
MsG0680034882.01.T01 MTR_2g025960 24.809 262 167 12 39 280 15 266 8.85e-19 85.5
MsG0680034882.01.T01 MTR_2g024100 24.812 266 170 8 39 282 106 363 8.98e-19 86.3
MsG0680034882.01.T01 MTR_2g426020 26.119 268 172 11 39 298 110 359 1.80e-18 85.5
MsG0680034882.01.T01 MTR_1g076810 36.478 159 79 6 9 155 93 241 3.40e-18 85.5
MsG0680034882.01.T01 MTR_2g426090 25.610 246 159 9 40 272 111 345 5.62e-18 84.0
MsG0680034882.01.T01 MTR_0667s0010 25.373 268 167 10 39 285 104 359 1.91e-17 82.4
MsG0680034882.01.T01 MTR_3g097190 49.367 79 37 1 227 302 28 106 2.60e-17 78.6
MsG0680034882.01.T01 MTR_1g068860 24.436 266 175 11 31 282 86 339 2.97e-17 81.6
MsG0680034882.01.T01 MTR_2g028160 24.430 307 190 12 16 294 89 381 3.48e-17 81.6
MsG0680034882.01.T01 MTR_3g047540 54.118 85 32 4 123 207 6 83 3.99e-17 75.5
MsG0680034882.01.T01 MTR_1g115205 32.584 178 81 6 1 160 79 235 9.00e-17 78.6
MsG0680034882.01.T01 MTR_3g083010 26.697 221 147 9 39 252 118 330 1.32e-16 80.1
MsG0680034882.01.T01 MTR_5g070080 26.259 278 149 11 51 305 136 380 2.99e-16 79.0
MsG0680034882.01.T01 MTR_1g050305 52.381 84 34 3 209 286 21 104 5.17e-16 73.9
MsG0680034882.01.T01 MTR_2g024110 24.232 293 174 14 36 302 93 363 3.44e-14 72.8
MsG0680034882.01.T01 MTR_2g031640 24.908 273 166 13 31 282 87 341 6.57e-14 72.0
MsG0680034882.01.T01 MTR_6g065980 27.200 250 150 11 39 288 113 330 2.14e-13 70.5
MsG0680034882.01.T01 MTR_1g097330 27.778 234 131 13 18 242 93 297 3.22e-13 70.1
MsG0680034882.01.T01 MTR_3g036335 25.424 236 142 11 39 270 145 350 5.27e-13 69.3
MsG0680034882.01.T01 MTR_5g021860 25.676 296 174 10 20 298 165 431 6.22e-13 69.3
MsG0680034882.01.T01 MTR_2g021150 27.092 251 144 12 27 263 105 330 6.39e-13 68.9
MsG0680034882.01.T01 MTR_5g021860 25.676 296 174 10 20 298 165 431 7.67e-13 69.3
MsG0680034882.01.T01 MTR_2g031720 22.794 272 171 12 32 282 92 345 8.16e-13 68.9
MsG0680034882.01.T01 MTR_3g006670 29.018 224 109 10 36 242 146 336 1.09e-12 68.6
MsG0680034882.01.T01 MTR_5g022420 24.390 246 156 7 35 279 121 337 1.12e-12 68.2
MsG0680034882.01.T01 MTR_2g031680 25.660 265 165 12 35 284 90 337 1.80e-12 67.8
MsG0680034882.01.T01 MTR_4g030830 25.877 228 125 7 36 242 112 316 3.50e-12 67.0
MsG0680034882.01.T01 MTR_6g060650 26.449 276 157 14 51 308 124 371 3.70e-12 66.6
MsG0680034882.01.T01 MTR_3g009240 27.397 219 127 9 32 243 124 317 6.24e-12 66.2
MsG0680034882.01.T01 MTR_2g029990 29.167 216 117 10 36 242 150 338 9.46e-12 65.5
MsG0680034882.01.T01 MTR_2g461450 24.242 264 155 8 23 265 95 334 9.80e-12 65.5
MsG0680034882.01.T01 MTR_4g027370 26.104 249 142 9 20 255 165 384 1.31e-11 65.5
MsG0680034882.01.T01 MTR_8g102520 25.573 262 157 11 55 301 75 313 1.31e-11 64.7
MsG0680034882.01.T01 MTR_8g090195 50.000 56 28 0 226 281 58 113 2.85e-11 60.1
MsG0680034882.01.T01 MTR_6g048120 26.000 300 183 11 14 308 117 382 3.27e-11 63.9
MsG0680034882.01.T01 MTR_5g010560 26.860 242 144 11 26 251 167 391 3.60e-11 63.9
MsG0680034882.01.T01 MTR_2g046830 25.571 219 135 8 27 242 115 308 3.83e-11 63.9
MsG0680034882.01.T01 MTR_3g013740 27.189 217 125 8 36 242 149 342 5.76e-11 63.2
MsG0680034882.01.T01 MTR_2g055970 26.941 219 131 9 30 242 301 496 6.03e-11 63.5
MsG0680034882.01.T01 MTR_4g094365 26.996 263 158 12 53 303 117 357 6.26e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034882.01.T01 AT3G06240 29.858 211 123 8 36 241 167 357 2.01e-17 82.8

Find 73 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGTGAGAGGTTTGAGAATA+AGG 0.185658 6:+96196372 MsG0680034882.01.T01:CDS
AGCGTGCTCGTTTGTGTAGC+TGG 0.269760 6:-96196514 None:intergenic
GTAAACTTGGCGGTGTTAAA+TGG 0.277192 6:+96196946 MsG0680034882.01.T01:CDS
AACCAACCTCCTCAATCATT+TGG 0.310877 6:-96197170 None:intergenic
GAAAAGATAAAGAAGTAGTT+TGG 0.341699 6:+96197133 MsG0680034882.01.T01:CDS
TTTGGTTTCGGTGGTATTGA+TGG 0.344664 6:+96196439 MsG0680034882.01.T01:CDS
CTCTCCACATGTTACTTCTT+AGG 0.348416 6:-96196723 None:intergenic
CGCATTGTTGTGGGTAAACT+TGG 0.352474 6:+96196933 MsG0680034882.01.T01:CDS
TTGGGTGAAGAAATCCCTTC+TGG 0.361562 6:+96196823 MsG0680034882.01.T01:CDS
CACGCTCCTTCCTCCCAGTC+TGG 0.367375 6:+96196531 MsG0680034882.01.T01:CDS
AAGGAAAACACTCTTCCATT+TGG 0.378090 6:+96197231 MsG0680034882.01.T01:CDS
TTTCACCATCATAATAATCA+TGG 0.398671 6:-96196474 None:intergenic
AATCCCTTCTGGATCATGTT+TGG 0.405213 6:+96196834 MsG0680034882.01.T01:CDS
AACTAGACCGGGAGCCAGAC+TGG 0.412351 6:-96196545 None:intergenic
ATCCTCTAGTTCTGAGGGTA+AGG 0.417409 6:-96196896 None:intergenic
TGGACCAAACTCTTCATTGT+TGG 0.433176 6:+96197048 MsG0680034882.01.T01:CDS
TAATTATTTATTCCTCCAAA+TGG 0.433360 6:-96197246 None:intergenic
TAGGCTATATATCTCCCATA+AGG 0.441236 6:-96196704 None:intergenic
ACCAACCTCCTCAATCATTT+GGG 0.446245 6:-96197169 None:intergenic
TGTTTCCATGATTATTATGA+TGG 0.449385 6:+96196469 MsG0680034882.01.T01:CDS
GCCGCTTTCTGTGGAATGTA+TGG 0.456965 6:+96196762 MsG0680034882.01.T01:CDS
CGGTCCAACAATGAAGAGTT+TGG 0.457196 6:-96197052 None:intergenic
TAAACTTGGCGGTGTTAAAT+GGG 0.459385 6:+96196947 MsG0680034882.01.T01:CDS
TCATGATTTCGCATTGTTGT+GGG 0.470021 6:+96196924 MsG0680034882.01.T01:CDS
GCGTGCTCGTTTGTGTAGCT+GGG 0.473936 6:-96196513 None:intergenic
GGATGGAGTGTGTCATTGGT+TGG 0.474472 6:+96196804 MsG0680034882.01.T01:CDS
CTTCCTCCCAGTCTGGCTCC+CGG 0.478149 6:+96196538 MsG0680034882.01.T01:CDS
CGGGAGCCAGACTGGGAGGA+AGG 0.478736 6:-96196537 None:intergenic
TTTAAGTACCCAAATGATTG+AGG 0.490285 6:+96197161 MsG0680034882.01.T01:CDS
ACATGGATGGAGTGTGTCAT+TGG 0.492746 6:+96196800 MsG0680034882.01.T01:CDS
TACCCTCAGAACTAGAGGAT+TGG 0.499269 6:+96196899 MsG0680034882.01.T01:CDS
TTCATGATTTCGCATTGTTG+TGG 0.500563 6:+96196923 MsG0680034882.01.T01:CDS
GTTGAGCTTCCTATCGGAGT+GGG 0.510966 6:+96197084 MsG0680034882.01.T01:CDS
TCTTGCGTTGAGCTTCCTAT+CGG 0.514385 6:+96197078 MsG0680034882.01.T01:CDS
GATGGAGTGTGTCATTGGTT+GGG 0.516709 6:+96196805 MsG0680034882.01.T01:CDS
TAAGATAAACTCAACTAGAC+CGG 0.518690 6:-96196557 None:intergenic
GAAAACACTCTTCCATTTGG+AGG 0.525879 6:+96197234 MsG0680034882.01.T01:CDS
TTGTTCGATTATAATTTACA+AGG 0.530543 6:+96197212 MsG0680034882.01.T01:CDS
AGGCTATATATCTCCCATAA+GGG 0.540514 6:-96196703 None:intergenic
TTTGGGTGAATTCGGTGTGA+AGG 0.549151 6:+96197020 MsG0680034882.01.T01:CDS
CCTTTCGTCCCCACTCCGAT+AGG 0.550231 6:-96197093 None:intergenic
GTTAAATGGGTCCATTGCAT+TGG 0.551884 6:+96196960 MsG0680034882.01.T01:CDS
ATAAAGTCATTTGTAATGTA+CGG 0.553002 6:+96196659 MsG0680034882.01.T01:CDS
GGACAGCATCCAAGTCTACA+TGG 0.555064 6:+96196783 MsG0680034882.01.T01:CDS
ACTAGACCGGGAGCCAGACT+GGG 0.560080 6:-96196544 None:intergenic
CTTTATAGTCATTTGTAACA+CGG 0.562412 6:-96196642 None:intergenic
AACCAATCCTCTAGTTCTGA+GGG 0.566095 6:-96196901 None:intergenic
TCCATACATTCCACAGAAAG+CGG 0.566858 6:-96196763 None:intergenic
CCTATCGGAGTGGGGACGAA+AGG 0.567512 6:+96197093 MsG0680034882.01.T01:CDS
GGTTGACATGCCGCTTTCTG+TGG 0.569843 6:+96196753 MsG0680034882.01.T01:CDS
AGCATCCAAGTCTACATGGA+TGG 0.570457 6:+96196787 MsG0680034882.01.T01:CDS
AAACCAATCCTCTAGTTCTG+AGG 0.574539 6:-96196902 None:intergenic
ACCCAAATGATTGAGGAGGT+TGG 0.575732 6:+96197168 MsG0680034882.01.T01:CDS
ATTGTTGTGGGTAAACTTGG+CGG 0.576268 6:+96196936 MsG0680034882.01.T01:CDS
ACACTCCATCCATGTAGACT+TGG 0.579628 6:-96196792 None:intergenic
CTTATTCTCAAACCTCTCAC+CGG 0.582453 6:-96196371 None:intergenic
CGTTGAGCTTCCTATCGGAG+TGG 0.593212 6:+96197083 MsG0680034882.01.T01:CDS
TAACATGTGGAGAGAACTTG+AGG 0.601549 6:+96196732 MsG0680034882.01.T01:CDS
ACCCTTACCCTCAGAACTAG+AGG 0.603935 6:+96196894 MsG0680034882.01.T01:CDS
TGTAATGTACGGTCAGCACG+AGG 0.607003 6:+96196670 MsG0680034882.01.T01:CDS
AAGATAAACTCAACTAGACC+GGG 0.608595 6:-96196556 None:intergenic
AAGTACCCAAATGATTGAGG+AGG 0.608686 6:+96197164 MsG0680034882.01.T01:CDS
GTCATGATATACCAATGCAA+TGG 0.610142 6:-96196971 None:intergenic
TCCTCTAGTTCTGAGGGTAA+GGG 0.616987 6:-96196895 None:intergenic
GATACCAAACATGATCCAGA+AGG 0.621545 6:-96196838 None:intergenic
GTTTATCTTAGATAATGATG+AGG 0.637843 6:+96196570 MsG0680034882.01.T01:CDS
AATTCGGTGTGAAGGAATCG+TGG 0.641995 6:+96197028 MsG0680034882.01.T01:CDS
TTGAGCTTCCTATCGGAGTG+GGG 0.661009 6:+96197085 MsG0680034882.01.T01:CDS
ATACCAAACATGATCCAGAA+GGG 0.678694 6:-96196837 None:intergenic
ATAGCCTAAGAAGTAACATG+TGG 0.680142 6:+96196719 MsG0680034882.01.T01:CDS
GGAAGCTCAACGCAAGACAA+CGG 0.681509 6:-96197072 None:intergenic
AGACCGGGAGCCAGACTGGG+AGG 0.702106 6:-96196541 None:intergenic
GTAATGTACGGTCAGCACGA+GGG 0.735811 6:+96196671 MsG0680034882.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTCACATATCAATTTT+GGG + Chr6:96197003-96197022 MsG0680034882.01.T01:CDS 15.0%
!!! TAATTTTCACATATCAATTT+TGG + Chr6:96197002-96197021 MsG0680034882.01.T01:CDS 15.0%
!! ATAAAGTCATTTGTAATGTA+CGG + Chr6:96196659-96196678 MsG0680034882.01.T01:CDS 20.0%
!! TAATTATTTATTCCTCCAAA+TGG - Chr6:96197249-96197268 None:intergenic 20.0%
!! TTGTTCGATTATAATTTACA+AGG + Chr6:96197212-96197231 MsG0680034882.01.T01:CDS 20.0%
!!! AATTTTCGTATTTTTGGTTT+CGG + Chr6:96196427-96196446 MsG0680034882.01.T01:CDS 20.0%
!!! GAGTTTTATATTCTTTTTTC+CGG + Chr6:96196352-96196371 MsG0680034882.01.T01:CDS 20.0%
! ATGGTGAAAAAATTGTATTG+TGG + Chr6:96196488-96196507 MsG0680034882.01.T01:CDS 25.0%
! CTTTATAGTCATTTGTAACA+CGG - Chr6:96196645-96196664 None:intergenic 25.0%
! GAAAAGATAAAGAAGTAGTT+TGG + Chr6:96197133-96197152 MsG0680034882.01.T01:CDS 25.0%
! GTTTATCTTAGATAATGATG+AGG + Chr6:96196570-96196589 MsG0680034882.01.T01:CDS 25.0%
! TGTTTCCATGATTATTATGA+TGG + Chr6:96196469-96196488 MsG0680034882.01.T01:CDS 25.0%
! TTTCACCATCATAATAATCA+TGG - Chr6:96196477-96196496 None:intergenic 25.0%
!! GAAGACAATTTTCGTATTTT+TGG + Chr6:96196421-96196440 MsG0680034882.01.T01:CDS 25.0%
!! TTTTATCATCTTCATGGATT+TGG + Chr6:96196613-96196632 MsG0680034882.01.T01:CDS 25.0%
!!! ATATCAATTTTGGGTGAATT+CGG + Chr6:96197012-96197031 MsG0680034882.01.T01:CDS 25.0%
!!! CGTATTTTTTATCATCTTCA+TGG + Chr6:96196607-96196626 MsG0680034882.01.T01:CDS 25.0%
TAAGATAAACTCAACTAGAC+CGG - Chr6:96196560-96196579 None:intergenic 30.0%
TTTAAGTACCCAAATGATTG+AGG + Chr6:96197161-96197180 MsG0680034882.01.T01:CDS 30.0%
! ATCGTTTTACTTTAGCAATG+AGG + Chr6:96196858-96196877 MsG0680034882.01.T01:CDS 30.0%
AAGATAAACTCAACTAGACC+GGG - Chr6:96196559-96196578 None:intergenic 35.0%
AAGGAAAACACTCTTCCATT+TGG + Chr6:96197231-96197250 MsG0680034882.01.T01:CDS 35.0%
AGGCTATATATCTCCCATAA+GGG - Chr6:96196706-96196725 None:intergenic 35.0%
ATACCAAACATGATCCAGAA+GGG - Chr6:96196840-96196859 None:intergenic 35.0%
ATAGCCTAAGAAGTAACATG+TGG + Chr6:96196719-96196738 MsG0680034882.01.T01:CDS 35.0%
GTCATGATATACCAATGCAA+TGG - Chr6:96196974-96196993 None:intergenic 35.0%
TAGGCTATATATCTCCCATA+AGG - Chr6:96196707-96196726 None:intergenic 35.0%
TCATGATTTCGCATTGTTGT+GGG + Chr6:96196924-96196943 MsG0680034882.01.T01:CDS 35.0%
TTCATGATTTCGCATTGTTG+TGG + Chr6:96196923-96196942 MsG0680034882.01.T01:CDS 35.0%
! TAAACTTGGCGGTGTTAAAT+GGG + Chr6:96196947-96196966 MsG0680034882.01.T01:CDS 35.0%
!!! ATATTCTTTTTTCCGGTGAG+AGG + Chr6:96196359-96196378 MsG0680034882.01.T01:CDS 35.0%
!!! TTTCGTATTTTTGGTTTCGG+TGG + Chr6:96196430-96196449 MsG0680034882.01.T01:CDS 35.0%
AACCAACCTCCTCAATCATT+TGG - Chr6:96197173-96197192 None:intergenic 40.0%
AAGTACCCAAATGATTGAGG+AGG + Chr6:96197164-96197183 MsG0680034882.01.T01:CDS 40.0%
AATCCCTTCTGGATCATGTT+TGG + Chr6:96196834-96196853 MsG0680034882.01.T01:CDS 40.0%
ACCAACCTCCTCAATCATTT+GGG - Chr6:96197172-96197191 None:intergenic 40.0%
ATTGTTGTGGGTAAACTTGG+CGG + Chr6:96196936-96196955 MsG0680034882.01.T01:CDS 40.0%
CTTATTCTCAAACCTCTCAC+CGG - Chr6:96196374-96196393 None:intergenic 40.0%
GAAAACACTCTTCCATTTGG+AGG + Chr6:96197234-96197253 MsG0680034882.01.T01:CDS 40.0%
GATACCAAACATGATCCAGA+AGG - Chr6:96196841-96196860 None:intergenic 40.0%
GTTAAATGGGTCCATTGCAT+TGG + Chr6:96196960-96196979 MsG0680034882.01.T01:CDS 40.0%
TAACATGTGGAGAGAACTTG+AGG + Chr6:96196732-96196751 MsG0680034882.01.T01:CDS 40.0%
TCCATACATTCCACAGAAAG+CGG - Chr6:96196766-96196785 None:intergenic 40.0%
TGGACCAAACTCTTCATTGT+TGG + Chr6:96197048-96197067 MsG0680034882.01.T01:CDS 40.0%
! CTCTCCACATGTTACTTCTT+AGG - Chr6:96196726-96196745 None:intergenic 40.0%
! GTAAACTTGGCGGTGTTAAA+TGG + Chr6:96196946-96196965 MsG0680034882.01.T01:CDS 40.0%
! TTTGGTTTCGGTGGTATTGA+TGG + Chr6:96196439-96196458 MsG0680034882.01.T01:CDS 40.0%
!! AAACCAATCCTCTAGTTCTG+AGG - Chr6:96196905-96196924 None:intergenic 40.0%
!! AACCAATCCTCTAGTTCTGA+GGG - Chr6:96196904-96196923 None:intergenic 40.0%
!!! AGGGTTTTTAGAGCCCTTAT+GGG + Chr6:96196690-96196709 MsG0680034882.01.T01:CDS 40.0%
ACACTCCATCCATGTAGACT+TGG - Chr6:96196795-96196814 None:intergenic 45.0%
ACATGGATGGAGTGTGTCAT+TGG + Chr6:96196800-96196819 MsG0680034882.01.T01:CDS 45.0%
ACCCAAATGATTGAGGAGGT+TGG + Chr6:96197168-96197187 MsG0680034882.01.T01:CDS 45.0%
AGCATCCAAGTCTACATGGA+TGG + Chr6:96196787-96196806 MsG0680034882.01.T01:CDS 45.0%
CGCATTGTTGTGGGTAAACT+TGG + Chr6:96196933-96196952 MsG0680034882.01.T01:CDS 45.0%
CGGTCCAACAATGAAGAGTT+TGG - Chr6:96197055-96197074 None:intergenic 45.0%
CGGTGAGAGGTTTGAGAATA+AGG + Chr6:96196372-96196391 MsG0680034882.01.T01:CDS 45.0%
TACCCTCAGAACTAGAGGAT+TGG + Chr6:96196899-96196918 MsG0680034882.01.T01:CDS 45.0%
TCTTGCGTTGAGCTTCCTAT+CGG + Chr6:96197078-96197097 MsG0680034882.01.T01:CDS 45.0%
TTGGGTGAAGAAATCCCTTC+TGG + Chr6:96196823-96196842 MsG0680034882.01.T01:CDS 45.0%
! GATGGAGTGTGTCATTGGTT+GGG + Chr6:96196805-96196824 MsG0680034882.01.T01:CDS 45.0%
!! AATTCGGTGTGAAGGAATCG+TGG + Chr6:96197028-96197047 MsG0680034882.01.T01:CDS 45.0%
!! ATCCTCTAGTTCTGAGGGTA+AGG - Chr6:96196899-96196918 None:intergenic 45.0%
!! TCCTCTAGTTCTGAGGGTAA+GGG - Chr6:96196898-96196917 None:intergenic 45.0%
!! TTTGGGTGAATTCGGTGTGA+AGG + Chr6:96197020-96197039 MsG0680034882.01.T01:CDS 45.0%
!!! GAGGGTTTTTAGAGCCCTTA+TGG + Chr6:96196689-96196708 MsG0680034882.01.T01:CDS 45.0%
ACCCTTACCCTCAGAACTAG+AGG + Chr6:96196894-96196913 MsG0680034882.01.T01:CDS 50.0%
GGAAGCTCAACGCAAGACAA+CGG - Chr6:96197075-96197094 None:intergenic 50.0%
GGACAGCATCCAAGTCTACA+TGG + Chr6:96196783-96196802 MsG0680034882.01.T01:CDS 50.0%
GTAATGTACGGTCAGCACGA+GGG + Chr6:96196671-96196690 MsG0680034882.01.T01:CDS 50.0%
GTTGAGCTTCCTATCGGAGT+GGG + Chr6:96197084-96197103 MsG0680034882.01.T01:CDS 50.0%
TGTAATGTACGGTCAGCACG+AGG + Chr6:96196670-96196689 MsG0680034882.01.T01:CDS 50.0%
TTGAGCTTCCTATCGGAGTG+GGG + Chr6:96197085-96197104 MsG0680034882.01.T01:CDS 50.0%
! GCCGCTTTCTGTGGAATGTA+TGG + Chr6:96196762-96196781 MsG0680034882.01.T01:CDS 50.0%
! GGATGGAGTGTGTCATTGGT+TGG + Chr6:96196804-96196823 MsG0680034882.01.T01:CDS 50.0%
AGCGTGCTCGTTTGTGTAGC+TGG - Chr6:96196517-96196536 None:intergenic 55.0%
CGTTGAGCTTCCTATCGGAG+TGG + Chr6:96197083-96197102 MsG0680034882.01.T01:CDS 55.0%
GCGTGCTCGTTTGTGTAGCT+GGG - Chr6:96196516-96196535 None:intergenic 55.0%
!! GGTTGACATGCCGCTTTCTG+TGG + Chr6:96196753-96196772 MsG0680034882.01.T01:CDS 55.0%
AACTAGACCGGGAGCCAGAC+TGG - Chr6:96196548-96196567 None:intergenic 60.0%
ACTAGACCGGGAGCCAGACT+GGG - Chr6:96196547-96196566 None:intergenic 60.0%
CCTATCGGAGTGGGGACGAA+AGG + Chr6:96197093-96197112 MsG0680034882.01.T01:CDS 60.0%
CCTTTCGTCCCCACTCCGAT+AGG - Chr6:96197096-96197115 None:intergenic 60.0%
CACGCTCCTTCCTCCCAGTC+TGG + Chr6:96196531-96196550 MsG0680034882.01.T01:CDS 65.0%
! CTTCCTCCCAGTCTGGCTCC+CGG + Chr6:96196538-96196557 MsG0680034882.01.T01:CDS 65.0%
AGACCGGGAGCCAGACTGGG+AGG - Chr6:96196544-96196563 None:intergenic 70.0%
CGGGAGCCAGACTGGGAGGA+AGG - Chr6:96196540-96196559 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr6 gene 96196343 96197269 96196343 ID=MsG0680034882.01;Name=MsG0680034882.01
Chr6 mRNA 96196343 96197269 96196343 ID=MsG0680034882.01.T01;Parent=MsG0680034882.01;Name=MsG0680034882.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|308
Chr6 exon 96196343 96197269 96196343 ID=MsG0680034882.01.T01:exon:6726;Parent=MsG0680034882.01.T01
Chr6 CDS 96196343 96197269 96196343 ID=MsG0680034882.01.T01:cds;Parent=MsG0680034882.01.T01
Gene Sequence

>MsG0680034882.01.T01

ATGATAAAAGAGTTTTATATTCTTTTTTCCGGTGAGAGGTTTGAGAATAAGGTTAAATTAGATTCGTTTGATGTATCTGAAGACAATTTTCGTATTTTTGGTTTCGGTGGTATTGATGGCACACTTTGTTTCCATGATTATTATGATGGTGAAAAAATTGTATTGTGGAACCCAGCTACACAAACGAGCACGCTCCTTCCTCCCAGTCTGGCTCCCGGTCTAGTTGAGTTTATCTTAGATAATGATGAGGATATGTATTTTGATCGTATTTTTTATCATCTTCATGGATTTGGTTATGACCGTGTTACAAATGACTATAAAGTCATTTGTAATGTACGGTCAGCACGAGGGTTTTTAGAGCCCTTATGGGAGATATATAGCCTAAGAAGTAACATGTGGAGAGAACTTGAGGTTGACATGCCGCTTTCTGTGGAATGTATGGACAGCATCCAAGTCTACATGGATGGAGTGTGTCATTGGTTGGGTGAAGAAATCCCTTCTGGATCATGTTTGGTATCGTTTTACTTTAGCAATGAGGTGTTCTTCACAACACCCTTACCCTCAGAACTAGAGGATTGGTTTCATGATTTCGCATTGTTGTGGGTAAACTTGGCGGTGTTAAATGGGTCCATTGCATTGGTATATCATGACGAGACGACTAATTTTCACATATCAATTTTGGGTGAATTCGGTGTGAAGGAATCGTGGACCAAACTCTTCATTGTTGGACCGTTGTCTTGCGTTGAGCTTCCTATCGGAGTGGGGACGAAAGGAGAAATATTCTTCATCAGAAAAGATAAAGAAGTAGTTTGGCTTGATTTAAGTACCCAAATGATTGAGGAGGTTGGTTATAAAGCATACAATCTTGATTGTTCGATTATAATTTACAAGGAAAACACTCTTCCATTTGGAGGAATAAATAATTAA
Protein sequence

>MsG0680034882.01.T01

MIKEFYILFSGERFENKVKLDSFDVSEDNFRIFGFGGIDGTLCFHDYYDGEKIVLWNPATQTSTLLPPSLAPGLVEFILDNDEDMYFDRIFYHLHGFGYDRVTNDYKVICNVRSARGFLEPLWEIYSLRSNMWRELEVDMPLSVECMDSIQVYMDGVCHWLGEEIPSGSCLVSFYFSNEVFFTTPLPSELEDWFHDFALLWVNLAVLNGSIALVYHDETTNFHISILGEFGVKESWTKLFIVGPLSCVELPIGVGTKGEIFFIRKDKEVVWLDLSTQMIEEVGYKAYNLDCSIIIYKENTLPFGGINN*