AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028716.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028716.01.T01 MTR_5g076490 78.804 368 37 3 1 365 3 332 0.0 540
MsG0580028716.01.T01 MTR_5g064700 60.471 382 116 12 1 365 1 364 8.51e-138 404
MsG0580028716.01.T01 MTR_5g097680 57.292 384 129 10 1 365 56 423 9.61e-129 383
MsG0580028716.01.T01 MTR_6g461660 51.882 372 141 12 1 366 58 397 1.34e-103 318
MsG0580028716.01.T01 MTR_6g086825 45.288 382 160 15 1 366 109 457 2.51e-85 272
MsG0580028716.01.T01 MTR_6g079770 45.119 379 173 13 1 365 56 413 6.58e-81 259
MsG0580028716.01.T01 MTR_0134s0060 43.008 379 173 13 3 363 53 406 5.92e-80 257
MsG0580028716.01.T01 MTR_6g090180 43.617 376 176 13 1 366 86 435 6.07e-79 255
MsG0580028716.01.T01 MTR_6g079810 45.103 388 153 16 1 365 109 459 6.14e-78 253
MsG0580028716.01.T01 MTR_5g094350 54.826 259 74 7 1 251 52 275 3.90e-74 237
MsG0580028716.01.T01 MTR_5g097330 42.105 380 172 11 1 366 55 400 4.89e-74 241
MsG0580028716.01.T01 MTR_6g478230 40.722 388 159 15 1 366 66 404 9.87e-74 241
MsG0580028716.01.T01 MTR_6g090250 41.253 383 197 13 1 366 50 421 5.82e-73 239
MsG0580028716.01.T01 MTR_5g097220 45.869 351 152 14 29 366 80 405 1.48e-72 238
MsG0580028716.01.T01 MTR_6g079790 44.048 336 145 12 40 364 1 304 1.54e-72 234
MsG0580028716.01.T01 MTR_0054s0100 43.122 378 175 14 1 366 56 405 2.38e-72 237
MsG0580028716.01.T01 MTR_4g028510 42.895 380 165 17 1 365 56 398 2.55e-72 238
MsG0580028716.01.T01 MTR_6g080680 44.412 340 145 9 28 363 90 389 1.87e-71 234
MsG0580028716.01.T01 MTR_0054s0090 42.257 381 172 15 1 366 56 403 9.23e-71 233
MsG0580028716.01.T01 MTR_6g078010 41.823 373 163 13 1 364 3 330 9.66e-71 231
MsG0580028716.01.T01 MTR_2g038680 41.953 379 180 14 1 365 55 407 9.71e-71 233
MsG0580028716.01.T01 MTR_4g130330 43.314 344 158 11 29 365 74 387 1.27e-69 229
MsG0580028716.01.T01 MTR_2g021800 42.216 379 166 12 8 365 66 412 1.82e-69 229
MsG0580028716.01.T01 MTR_0129s0070 41.935 372 174 14 1 365 56 392 1.12e-68 227
MsG0580028716.01.T01 MTR_6g087620 41.543 337 133 9 29 359 1 279 5.90e-67 218
MsG0580028716.01.T01 MTR_4g102410 41.192 386 167 15 1 365 100 446 9.58e-66 221
MsG0580028716.01.T01 MTR_4g028680 41.270 378 173 15 1 365 55 396 1.06e-65 219
MsG0580028716.01.T01 MTR_2g022000 39.702 403 187 12 1 379 55 425 3.27e-65 219
MsG0580028716.01.T01 MTR_4g028660 39.481 385 171 16 1 366 55 396 3.27e-65 218
MsG0580028716.01.T01 MTR_4g027450 39.481 385 171 16 1 366 55 396 3.27e-65 218
MsG0580028716.01.T01 MTR_8g041270 44.669 347 153 13 29 365 85 402 4.18e-65 218
MsG0580028716.01.T01 MTR_0129s0120 41.737 357 169 13 14 366 72 393 5.78e-65 217
MsG0580028716.01.T01 MTR_8g464770 41.033 368 150 12 8 363 62 374 6.67e-65 216
MsG0580028716.01.T01 MTR_0129s0050 40.526 380 183 15 1 365 53 404 2.04e-64 216
MsG0580028716.01.T01 MTR_2g104340 42.105 380 161 17 5 365 53 392 1.09e-63 214
MsG0580028716.01.T01 MTR_8g063440 41.489 376 162 16 1 365 51 379 2.79e-63 212
MsG0580028716.01.T01 MTR_2g021950 39.947 378 169 15 4 365 58 393 1.58e-61 208
MsG0580028716.01.T01 MTR_7g017040 40.849 377 174 14 1 365 55 394 2.11e-61 208
MsG0580028716.01.T01 MTR_5g037680 40.310 387 154 16 1 363 47 380 3.12e-61 209
MsG0580028716.01.T01 MTR_4g014370 37.722 395 174 14 3 366 53 406 4.25e-59 202
MsG0580028716.01.T01 MTR_7g111700 40.591 372 157 14 5 365 43 361 7.41e-59 200
MsG0580028716.01.T01 MTR_4g007940 39.773 352 162 12 29 368 28 341 7.74e-59 199
MsG0580028716.01.T01 MTR_2g104280 39.155 355 160 14 30 365 84 401 1.05e-58 201
MsG0580028716.01.T01 MTR_8g033140 38.303 389 180 14 1 363 58 412 1.44e-58 201
MsG0580028716.01.T01 MTR_1g115205 55.122 205 46 5 31 230 86 249 3.75e-58 194
MsG0580028716.01.T01 MTR_4g117550 37.067 375 161 15 1 363 56 367 7.32e-58 197
MsG0580028716.01.T01 MTR_5g092860 38.440 359 162 12 1 347 41 352 1.87e-57 196
MsG0580028716.01.T01 MTR_5g069390 39.276 359 161 15 29 372 104 420 1.95e-57 200
MsG0580028716.01.T01 MTR_4g028750 38.838 327 164 9 28 350 86 380 4.07e-57 196
MsG0580028716.01.T01 MTR_6g090260 54.688 192 80 5 179 366 73 261 5.02e-57 192
MsG0580028716.01.T01 MTR_5g059430 40.057 352 160 15 29 366 109 423 5.19e-57 197
MsG0580028716.01.T01 MTR_5g074800 39.516 372 178 15 1 361 1 336 9.26e-57 194
MsG0580028716.01.T01 MTR_7g017130 39.118 340 169 12 28 365 90 393 2.06e-56 194
MsG0580028716.01.T01 MTR_2g021770 37.696 382 186 15 4 365 54 403 2.21e-56 195
MsG0580028716.01.T01 MTR_3g011120 38.147 367 162 14 4 358 56 369 3.61e-56 193
MsG0580028716.01.T01 MTR_7g069550 39.578 379 171 17 1 361 53 391 5.55e-56 193
MsG0580028716.01.T01 MTR_1g053510 38.579 394 178 18 1 370 54 407 1.18e-55 193
MsG0580028716.01.T01 MTR_7g017170 38.462 351 168 13 28 365 90 405 1.57e-55 192
MsG0580028716.01.T01 MTR_1g007450 38.482 369 176 15 1 355 51 382 8.22e-55 191
MsG0580028716.01.T01 MTR_4g055820 44.481 308 122 14 29 323 87 358 1.54e-54 188
MsG0580028716.01.T01 MTR_5g096630 36.990 392 184 14 1 366 57 411 8.14e-54 188
MsG0580028716.01.T01 MTR_5g096910 37.861 346 161 13 29 365 89 389 8.26e-54 187
MsG0580028716.01.T01 MTR_0831s0010 37.861 346 158 10 27 365 122 417 1.33e-53 187
MsG0580028716.01.T01 MTR_8g064890 39.130 345 164 12 25 365 82 384 1.42e-53 187
MsG0580028716.01.T01 MTR_8g467670 37.183 355 169 11 12 362 68 372 1.43e-53 187
MsG0580028716.01.T01 MTR_5g097350 37.337 383 170 15 1 366 55 384 2.86e-53 186
MsG0580028716.01.T01 MTR_5g092850 38.728 346 167 13 32 365 86 398 5.59e-52 183
MsG0580028716.01.T01 MTR_5g043605 37.427 342 155 14 30 364 45 334 7.61e-52 181
MsG0580028716.01.T01 MTR_8g046200 38.162 359 169 14 32 364 86 417 8.32e-52 183
MsG0580028716.01.T01 MTR_2g021920 45.344 247 109 9 131 365 37 269 9.50e-52 178
MsG0580028716.01.T01 MTR_2g066200 36.000 375 189 12 1 365 51 384 1.09e-51 182
MsG0580028716.01.T01 MTR_1g007410 39.067 343 163 13 27 361 94 398 1.17e-51 182
MsG0580028716.01.T01 MTR_5g022850 38.439 346 167 16 29 365 41 349 6.97e-51 178
MsG0580028716.01.T01 MTR_8g046310 38.667 375 163 17 1 364 55 373 1.13e-50 179
MsG0580028716.01.T01 MTR_8g033090 35.897 390 187 16 1 363 53 406 1.19e-50 179
MsG0580028716.01.T01 MTR_8g012090 37.681 345 142 10 1 337 55 334 2.21e-50 176
MsG0580028716.01.T01 MTR_2g065840 34.828 379 189 13 1 365 51 385 4.03e-50 177
MsG0580028716.01.T01 MTR_2g065860 36.257 342 174 13 29 365 82 384 4.45e-50 177
MsG0580028716.01.T01 MTR_6g004450 46.502 243 108 9 126 366 44 266 1.14e-49 172
MsG0580028716.01.T01 MTR_2g034700 38.012 342 141 13 32 362 88 369 6.22e-49 174
MsG0580028716.01.T01 MTR_7g023800 36.585 369 158 13 1 360 1 302 6.54e-49 172
MsG0580028716.01.T01 MTR_2g066170 37.609 343 163 14 29 364 83 381 2.81e-48 172
MsG0580028716.01.T01 MTR_5g074850 38.053 339 159 15 30 352 105 408 2.91e-48 180
MsG0580028716.01.T01 MTR_5g091140 36.957 368 151 14 1 346 87 395 2.99e-48 173
MsG0580028716.01.T01 MTR_8g046220 35.190 395 176 16 1 364 56 401 3.11e-48 173
MsG0580028716.01.T01 MTR_2g066150 37.209 344 144 13 29 365 78 356 2.38e-47 169
MsG0580028716.01.T01 MTR_8g018110 33.938 386 185 17 1 363 53 391 4.16e-47 169
MsG0580028716.01.T01 MTR_5g075370 31.818 484 255 22 30 482 78 517 4.73e-46 173
MsG0580028716.01.T01 MTR_1g007430 38.957 326 148 10 7 326 44 324 1.26e-45 164
MsG0580028716.01.T01 MTR_3g018750 43.028 251 115 9 129 365 1 237 1.35e-44 158
MsG0580028716.01.T01 MTR_5g023970 49.231 195 88 6 176 366 6 193 1.40e-44 156
MsG0580028716.01.T01 MTR_5g086590 40.078 257 111 8 89 340 13 231 3.25e-44 157
MsG0580028716.01.T01 MTR_8g033110 35.623 393 193 21 1 366 53 412 6.01e-44 161
MsG0580028716.01.T01 MTR_7g079640 38.630 365 151 18 1 350 52 358 9.66e-44 160
MsG0580028716.01.T01 MTR_5g024260 39.222 334 146 16 29 346 92 384 1.90e-42 157
MsG0580028716.01.T01 MTR_1g098180 34.383 381 154 18 1 363 58 360 2.36e-42 155
MsG0580028716.01.T01 MTR_5g068490 35.061 328 149 11 38 364 8 272 4.93e-42 152
MsG0580028716.01.T01 MTR_4g014370 44.393 214 100 5 160 366 133 334 4.94e-42 154
MsG0580028716.01.T01 MTR_6g079730 56.849 146 59 3 222 366 5 147 9.81e-42 147
MsG0580028716.01.T01 MTR_8g064790 35.088 342 133 11 25 365 80 333 3.91e-41 152
MsG0580028716.01.T01 MTR_8g033000 35.359 362 175 15 29 363 28 357 1.42e-40 150
MsG0580028716.01.T01 MTR_5g097850 48.936 188 79 6 186 365 63 241 7.96e-40 145
MsG0580028716.01.T01 MTR_8g032980 32.891 377 172 15 28 363 79 415 9.06e-40 150
MsG0580028716.01.T01 MTR_5g072060 36.813 364 157 15 1 347 45 352 2.17e-39 152
MsG0580028716.01.T01 MTR_8g039500 35.494 324 140 11 29 337 77 346 2.80e-39 147
MsG0580028716.01.T01 MTR_1g050312 34.242 330 141 11 29 350 16 277 5.03e-39 144
MsG0580028716.01.T01 MTR_8g036970 46.789 218 87 10 180 392 146 339 5.64e-39 147
MsG0580028716.01.T01 MTR_3g030620 45.783 166 82 3 202 363 15 176 8.66e-39 140
MsG0580028716.01.T01 MTR_8g035690 36.735 294 135 10 95 363 30 297 1.12e-38 144
MsG0580028716.01.T01 MTR_5g098950 40.586 239 87 6 90 326 89 274 1.73e-37 140
MsG0580028716.01.T01 MTR_7g016930 37.500 304 132 13 1 300 55 304 4.27e-37 140
MsG0580028716.01.T01 MTR_8g063330 35.314 303 145 12 29 323 89 348 2.32e-36 141
MsG0580028716.01.T01 MTR_5g096900 37.942 311 126 11 1 292 51 313 5.22e-36 137
MsG0580028716.01.T01 MTR_8g063350 33.429 350 164 15 25 359 41 336 8.15e-36 137
MsG0580028716.01.T01 MTR_2g064950 34.737 380 180 19 1 360 49 380 3.97e-35 136
MsG0580028716.01.T01 MTR_8g013000 36.232 345 161 15 1 326 49 353 4.04e-35 137
MsG0580028716.01.T01 MTR_6g009350 41.395 215 99 9 124 331 1 195 5.30e-35 130
MsG0580028716.01.T01 MTR_7g104710 36.913 298 107 12 1 284 56 286 5.42e-35 134
MsG0580028716.01.T01 MTR_5g066420 49.342 152 71 5 210 358 1 149 1.50e-34 127
MsG0580028716.01.T01 MTR_4g016000 39.407 236 97 10 94 325 2 195 3.30e-34 129
MsG0580028716.01.T01 MTR_8g063370 34.024 338 166 18 32 360 94 383 1.79e-33 132
MsG0580028716.01.T01 MTR_1g054775 38.333 180 103 1 367 538 1030 1209 8.11e-33 134
MsG0580028716.01.T01 MTR_7g017150 41.837 196 105 4 174 366 75 264 2.63e-31 122
MsG0580028716.01.T01 MTR_7g019250 36.475 244 118 4 103 345 206 413 2.71e-31 127
MsG0580028716.01.T01 MTR_7g017120 40.816 196 107 4 174 366 74 263 3.52e-30 119
MsG0580028716.01.T01 MTR_2g104260 36.036 222 106 7 30 243 84 277 4.98e-27 110
MsG0580028716.01.T01 MTR_5g096920 39.873 158 83 6 210 365 1 148 2.13e-25 102
MsG0580028716.01.T01 MTR_5g097660 51.923 104 47 2 265 366 15 117 6.48e-25 100
MsG0580028716.01.T01 MTR_8g063310 31.579 285 145 13 92 361 140 389 1.05e-23 103
MsG0580028716.01.T01 MTR_5g097540 38.547 179 93 7 153 326 67 233 3.06e-23 100
MsG0580028716.01.T01 MTR_2g022020 51.456 103 46 2 264 365 17 116 4.46e-23 94.7
MsG0580028716.01.T01 MTR_3g102690 30.588 170 107 2 375 533 16 185 5.62e-23 97.1
MsG0580028716.01.T01 MTR_5g036250 61.446 83 29 3 280 361 23 103 5.89e-22 94.0
MsG0580028716.01.T01 MTR_7g101200 33.607 244 102 11 89 325 38 228 2.20e-21 93.6
MsG0580028716.01.T01 MTR_4g094400 50.000 106 40 2 208 302 8 111 9.81e-21 88.6
MsG0580028716.01.T01 MTR_2g437490 30.921 152 95 2 375 518 41 190 9.27e-20 88.2
MsG0580028716.01.T01 MTR_1g050308 27.778 342 139 12 29 365 105 343 5.04e-19 89.0
MsG0580028716.01.T01 MTR_4g133170 26.616 263 152 10 85 338 100 330 6.37e-19 89.4
MsG0580028716.01.T01 MTR_5g086780 43.902 123 58 3 227 344 112 228 7.91e-19 86.3
MsG0580028716.01.T01 MTR_1g076810 35.238 210 96 10 5 208 61 236 2.00e-18 89.4
MsG0580028716.01.T01 MTR_6g070880 32.367 207 116 7 1 201 71 259 3.66e-18 85.1
MsG0580028716.01.T01 MTR_6g071420 32.367 207 116 7 1 201 71 259 3.66e-18 85.1
MsG0580028716.01.T01 MTR_7g111367 26.230 183 114 5 311 475 11 190 7.78e-17 82.4
MsG0580028716.01.T01 MTR_5g097820 31.301 246 120 10 1 235 62 269 2.13e-16 80.1
MsG0580028716.01.T01 MTR_2g031640 26.644 289 167 13 91 367 107 362 4.32e-16 80.9
MsG0580028716.01.T01 MTR_1g047080 26.415 371 171 17 1 364 3 278 7.56e-16 78.6
MsG0580028716.01.T01 MTR_5g037190 33.175 211 92 10 28 237 88 250 9.92e-16 77.4
MsG0580028716.01.T01 MTR_6g017295 31.159 138 87 1 365 494 410 547 2.12e-15 79.7
MsG0580028716.01.T01 MTR_1g026690 31.061 132 83 1 376 499 57 188 2.59e-15 78.2
MsG0580028716.01.T01 MTR_6g465360 32.039 206 112 10 1 197 101 287 3.50e-15 76.6
MsG0580028716.01.T01 MTR_8g442690 34.416 154 82 4 370 506 326 477 8.86e-15 77.4
MsG0580028716.01.T01 MTR_7g079370 27.622 286 165 10 90 365 96 349 8.93e-15 76.3
MsG0580028716.01.T01 MTR_5g096050 56.098 82 32 3 231 311 30 108 1.22e-14 70.9
MsG0580028716.01.T01 MTR_3g047540 52.113 71 28 3 180 249 5 70 1.48e-14 69.7
MsG0580028716.01.T01 MTR_2g090750 31.579 133 83 1 375 499 309 441 2.33e-14 76.6
MsG0580028716.01.T01 MTR_0115s0020 26.688 311 168 14 77 365 123 395 3.84e-14 74.7
MsG0580028716.01.T01 MTR_7g073570 31.967 122 75 1 394 507 138 259 4.17e-14 74.7
MsG0580028716.01.T01 MTR_6g006375 27.517 149 96 3 394 533 57 202 4.83e-14 72.4
MsG0580028716.01.T01 MTR_3g435820 31.933 119 73 1 397 507 11 129 1.17e-13 72.4
MsG0580028716.01.T01 MTR_2g064455 31.707 164 104 3 376 538 41 197 1.23e-13 71.2
MsG0580028716.01.T01 MTR_1g068835 23.462 260 158 9 91 340 106 334 1.47e-13 72.8
MsG0580028716.01.T01 MTR_2g026010 25.926 324 189 17 85 391 100 389 1.95e-13 72.8
MsG0580028716.01.T01 MTR_2g031720 25.660 265 151 12 91 341 111 343 4.77e-13 71.6
MsG0580028716.01.T01 MTR_8g015630 30.952 126 79 1 365 482 53 178 8.71e-13 70.1
MsG0580028716.01.T01 MTR_0667s0010 25.448 279 152 12 85 341 113 357 1.24e-12 70.1
MsG0580028716.01.T01 MTR_3g097190 53.704 54 24 1 289 341 28 81 1.36e-12 67.0
MsG0580028716.01.T01 MTR_5g048160 26.733 303 165 16 85 365 121 388 7.14e-12 67.8
MsG0580028716.01.T01 MTR_0064s0160 25.667 300 170 15 77 363 158 417 1.51e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028716.01.T01 AT3G24255 32.609 138 84 2 375 504 26 162 2.16e-17 85.5
MsG0580028716.01.T01 AT3G24255 32.609 138 84 2 375 504 80 216 2.17e-17 85.9
MsG0580028716.01.T01 AT3G24255 32.609 138 84 2 375 504 80 216 2.17e-17 85.9
MsG0580028716.01.T01 AT3G24255 32.609 138 84 2 375 504 80 216 5.23e-17 85.1
MsG0580028716.01.T01 AT3G24255 30.000 140 89 2 375 506 26 164 4.43e-14 75.9

Find 124 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGCGTACATATGACTTTA+AGG 0.175449 5:+85300672 MsG0580028716.01.T01:CDS
TGGATGTGGTAAATTTAATT+TGG 0.204224 5:-85300386 None:intergenic
CTTTGGTTCAAGGTGCTTAA+AGG 0.206323 5:+85302421 MsG0580028716.01.T01:CDS
GGAAAGAATTAACCGAATTA+TGG 0.213480 5:+85302509 MsG0580028716.01.T01:CDS
CCGTTCGCTGCAAATGTTAA+TGG 0.225920 5:+85300598 MsG0580028716.01.T01:CDS
AATAAATCCATCTATGTCTA+TGG 0.233226 5:-85301004 None:intergenic
AAATTGAGAGAGCCTTTATC+TGG 0.265750 5:+85302242 MsG0580028716.01.T01:CDS
CACCCAAGTCGTCAAGTTCA+TGG 0.286151 5:+85300637 MsG0580028716.01.T01:CDS
CTCTAAAAGAGAATCAAATT+TGG 0.290167 5:+85300936 MsG0580028716.01.T01:CDS
ATCTCTTCCAAACAACTCTT+TGG 0.301723 5:-85302214 None:intergenic
CAAACAACTCTTTGGCATCT+TGG 0.302651 5:-85302206 None:intergenic
CACCAAGGTCCTCAATCATT+TGG 0.303718 5:-85301275 None:intergenic
AGGTATCATTAGAACCCTTC+TGG 0.305119 5:+85300785 MsG0580028716.01.T01:CDS
TAACCTGATCTAAAACAAAT+TGG 0.305974 5:-85302102 None:intergenic
ATACATCAATAGAACGCTTC+TGG 0.317328 5:+85300761 MsG0580028716.01.T01:CDS
CCCAGTCCGCCAACATCTTC+AGG 0.320297 5:-85302322 None:intergenic
AATTAACCGAATTATGGATT+TGG 0.320385 5:+85302515 MsG0580028716.01.T01:CDS
AGTCGTCAAGTTCATGGATT+TGG 0.329651 5:+85300643 MsG0580028716.01.T01:CDS
GGTTAATTCTTTCCAAAGTC+TGG 0.333649 5:-85302500 None:intergenic
CGAAATTTAAGATTTCAAAA+TGG 0.345845 5:-85300435 None:intergenic
CCTGAAGATGTTGGCGGACT+GGG 0.348435 5:+85302322 MsG0580028716.01.T01:CDS
ACAATGGAAAGAATGATCTT+TGG 0.348788 5:+85302404 MsG0580028716.01.T01:CDS
ATCTGTATCACCCCAGATAA+AGG 0.350971 5:-85302254 None:intergenic
TCATGTGTCGAGTGTCCTTT+TGG 0.369050 5:+85301186 MsG0580028716.01.T01:CDS
TTGTTTGTTGAAATTAGCTT+GGG 0.383700 5:+85302375 MsG0580028716.01.T01:CDS
CTGCTGTAAGATTTGCACTA+TGG 0.385263 5:+85300521 MsG0580028716.01.T01:CDS
ACCTGAAGATGTTGGCGGAC+TGG 0.386158 5:+85302321 MsG0580028716.01.T01:CDS
ATCGTATTCCCACATGTTGT+TGG 0.388995 5:-85300859 None:intergenic
GTTTCAGTTCCTTCTTGAAA+TGG 0.391484 5:-85300406 None:intergenic
ATGTAGACAATAAGTATTCA+TGG 0.395776 5:+85301028 MsG0580028716.01.T01:CDS
GGATCAAACTCTTCATTGTT+GGG 0.398673 5:+85301157 MsG0580028716.01.T01:CDS
CTTGAATGGACTGTGTCATT+GGG 0.401379 5:+85300891 MsG0580028716.01.T01:CDS
CAAGATGCCAAAGAGTTGTT+TGG 0.403946 5:+85302207 MsG0580028716.01.T01:CDS
TCCATCTATGTCTATGGGTA+TGG 0.405397 5:-85300998 None:intergenic
GCGAAACCAAATCCATAATT+CGG 0.410245 5:-85302521 None:intergenic
TGGATCAAACTCTTCATTGT+TGG 0.415851 5:+85301156 MsG0580028716.01.T01:CDS
ACTTGAATGGACTGTGTCAT+TGG 0.421413 5:+85300890 MsG0580028716.01.T01:CDS
TTCCAAAGTCTGGAGTTTGT+AGG 0.422989 5:-85302490 None:intergenic
CTTTAAGGTGATTAGTTATG+TGG 0.424774 5:+85300687 MsG0580028716.01.T01:CDS
AAGGAAAACCTTCTTCCAAT+TGG 0.426058 5:+85301339 MsG0580028716.01.T01:CDS
CACGGGGTAGACCTGACATA+TGG 0.428435 5:+85300317 MsG0580028716.01.T01:CDS
CGCAAAGGTTATGTGGCATT+AGG 0.434781 5:+85300736 MsG0580028716.01.T01:CDS
ATAGTTTAAGAAGTAACTCT+TGG 0.438413 5:+85300815 MsG0580028716.01.T01:CDS
ACTTTGAATTCGTCCGTAGT+TGG 0.440008 5:-85300547 None:intergenic
TGTCAGATAAGAAATTGTTA+TGG 0.442968 5:-85300270 None:intergenic
CCCATACCCATAGACATAGA+TGG 0.443690 5:+85300997 MsG0580028716.01.T01:CDS
ACCAAGGTCCTCAATCATTT+GGG 0.448104 5:-85301274 None:intergenic
TACATCAATAGAACGCTTCT+GGG 0.450365 5:+85300762 MsG0580028716.01.T01:CDS
AACCTACAAACTCCAGACTT+TGG 0.456227 5:+85302488 MsG0580028716.01.T01:CDS
CTTGTTTGTTGAAATTAGCT+TGG 0.456746 5:+85302374 MsG0580028716.01.T01:CDS
TTGTTCTTGATATGTGATCA+AGG 0.459685 5:-85301082 None:intergenic
TTGCCCTTTCCCACTCCAAA+AGG 0.464318 5:-85301201 None:intergenic
CTAATTTCAACAAACAAGCT+TGG 0.467379 5:-85302369 None:intergenic
TAAGGTACATGCAGTTCGTT+GGG 0.476867 5:+85302285 MsG0580028716.01.T01:CDS
AAAGAATGATCTTTGGTTCA+AGG 0.477965 5:+85302411 MsG0580028716.01.T01:CDS
TTTAAGTACCCAAATGATTG+AGG 0.479377 5:+85301266 MsG0580028716.01.T01:CDS
GAGGACCTTGGTGTTAAAAG+AGG 0.481306 5:+85301285 MsG0580028716.01.T01:CDS
GATCACATATCAAGAACAAA+TGG 0.488807 5:+85301086 MsG0580028716.01.T01:CDS
CAAAGTCATTCCTCATAGTC+CGG 0.492741 5:+85300564 MsG0580028716.01.T01:CDS
AATCCATGAACTTGACGACT+TGG 0.496122 5:-85300640 None:intergenic
TTATTCCACCAATTGGAAGA+AGG 0.496695 5:-85301347 None:intergenic
AATTAACTGTGAAGGAATCA+TGG 0.499475 5:+85301136 MsG0580028716.01.T01:CDS
ACGATGGGAGTGTACTTGAA+TGG 0.500005 5:+85300877 MsG0580028716.01.T01:CDS
TATATCTCTCATCTTATCAC+GGG 0.506497 5:+85300300 MsG0580028716.01.T01:CDS
TTTGAGTGAATTAACTGTGA+AGG 0.506731 5:+85301128 MsG0580028716.01.T01:CDS
GGTATCATTAGAACCCTTCT+GGG 0.507446 5:+85300786 MsG0580028716.01.T01:CDS
CATAGTGCAAATCTTACAGC+AGG 0.507506 5:-85300520 None:intergenic
TACATTCTCAAATCTTTCGC+CGG 0.509200 5:-85300362 None:intergenic
AATTGAGAGAGCCTTTATCT+GGG 0.513379 5:+85302243 MsG0580028716.01.T01:CDS
CGAGGCGCAATCTCAGTCAA+TGG 0.526947 5:-85302067 None:intergenic
GTTCCTTCTTGAAATGGATG+TGG 0.527676 5:-85300400 None:intergenic
TTAGAGAAGCTGTGTCGCTA+TGG 0.527870 5:+85302029 MsG0580028716.01.T01:CDS
GTTTAAGAAGTAACTCTTGG+AGG 0.529321 5:+85300818 MsG0580028716.01.T01:CDS
CTTTGCGTGGATTCAATCGT+GGG 0.530835 5:-85300720 None:intergenic
CCATCTATGTCTATGGGTAT+GGG 0.531881 5:-85300997 None:intergenic
TTGATATGTGATCAAGGCAA+TGG 0.533113 5:-85301076 None:intergenic
TAAACTATATATCTCCCAGA+AGG 0.534106 5:-85300800 None:intergenic
ACGGGGTAGACCTGACATAT+GGG 0.536056 5:+85300318 MsG0580028716.01.T01:CDS
GTAAGGTACATGCAGTTCGT+TGG 0.536907 5:+85302284 MsG0580028716.01.T01:CDS
TTAGCTTGGGGTTTGTACAA+TGG 0.537329 5:+85302388 MsG0580028716.01.T01:CDS
ATAAATCCATCTATGTCTAT+GGG 0.540093 5:-85301003 None:intergenic
ATATATCTCTCATCTTATCA+CGG 0.540680 5:+85300299 MsG0580028716.01.T01:CDS
CCATTAACATTTGCAGCGAA+CGG 0.547358 5:-85300598 None:intergenic
AAAACTCCCACCCATATGTC+AGG 0.550990 5:-85300328 None:intergenic
CCTTTGCGTGGATTCAATCG+TGG 0.557292 5:-85300721 None:intergenic
GAATCCACGCAAAGGTTATG+TGG 0.560830 5:+85300729 MsG0580028716.01.T01:CDS
TTACCACATCCATTTCAAGA+AGG 0.563082 5:+85300397 MsG0580028716.01.T01:CDS
GTATTCATGGAGAGACTTGG+CGG 0.565778 5:+85301041 MsG0580028716.01.T01:CDS
ATTACGAGACCTGAAGATGT+TGG 0.570889 5:+85302313 MsG0580028716.01.T01:CDS
ATCCATGAACTTGACGACTT+GGG 0.570997 5:-85300639 None:intergenic
TTGAATGGACTGTGTCATTG+GGG 0.572280 5:+85300892 MsG0580028716.01.T01:CDS
TGCACTATGGAATCCAACTA+CGG 0.572611 5:+85300534 MsG0580028716.01.T01:CDS
TTGTGAGATAGTAGTGTACA+AGG 0.575887 5:+85301320 MsG0580028716.01.T01:CDS
TGAAACATATCCGGACTATG+AGG 0.576466 5:-85300574 None:intergenic
GTGTCGCTATGGAAGTATCT+TGG 0.579503 5:+85302040 MsG0580028716.01.T01:CDS
GCGAACGGTTGAAACATATC+CGG 0.588476 5:-85300583 None:intergenic
TTAAAGGAAAATATGAGTGT+GGG 0.595838 5:+85302437 MsG0580028716.01.T01:CDS
TAAGTATTCATGGAGAGACT+TGG 0.597902 5:+85301038 MsG0580028716.01.T01:CDS
TATGTCTATGGGTATGGGTG+TGG 0.599534 5:-85300992 None:intergenic
AATGCCACATAACCTTTGCG+TGG 0.605583 5:-85300733 None:intergenic
CTTAAAGGAAAATATGAGTG+TGG 0.609192 5:+85302436 MsG0580028716.01.T01:CDS
ACGAGACCTGAAGATGTTGG+CGG 0.609675 5:+85302316 MsG0580028716.01.T01:CDS
TGTTTGTTGAAATTAGCTTG+GGG 0.610345 5:+85302376 MsG0580028716.01.T01:CDS
TGATACAGATCATATTCGTA+AGG 0.613237 5:+85302267 MsG0580028716.01.T01:CDS
ACAACATGTGGGAATACGAT+GGG 0.622217 5:+85300862 MsG0580028716.01.T01:CDS
ACCCAAATGATTGAGGACCT+TGG 0.628747 5:+85301273 MsG0580028716.01.T01:CDS
AACAACATGTGGGAATACGA+TGG 0.634664 5:+85300861 MsG0580028716.01.T01:CDS
ATTGAGAGAGCCTTTATCTG+GGG 0.642746 5:+85302244 MsG0580028716.01.T01:CDS
GGGTAGACCTGACATATGGG+TGG 0.650130 5:+85300321 MsG0580028716.01.T01:CDS
AAACTATATATCTCCCAGAA+GGG 0.651709 5:-85300799 None:intergenic
GTTAAAAGAGGAAATAGTCG+TGG 0.652729 5:+85301297 MsG0580028716.01.T01:CDS
AATTGGAAATCTGATTTACG+AGG 0.669793 5:-85302085 None:intergenic
CCACGATTGAATCCACGCAA+AGG 0.669835 5:+85300721 MsG0580028716.01.T01:CDS
CCTTAAAGTCATATGTACGC+AGG 0.670924 5:-85300672 None:intergenic
GGTAGACCTGACATATGGGT+GGG 0.672892 5:+85300322 MsG0580028716.01.T01:CDS
AAGCTTGGTTCATGACAGTG+AGG 0.678748 5:-85302354 None:intergenic
ATAAGATGAGAGATATATCG+TGG 0.679943 5:-85300294 None:intergenic
GTTGTTATGCCAACAACATG+TGG 0.680161 5:+85300850 MsG0580028716.01.T01:CDS
TTGTTATGCCAACAACATGT+GGG 0.683967 5:+85300851 MsG0580028716.01.T01:CDS
TTTGCGTGGATTCAATCGTG+GGG 0.691928 5:-85300719 None:intergenic
ATCAATAGAACGCTTCTGGG+AGG 0.693716 5:+85300765 MsG0580028716.01.T01:CDS
TGAATGGACTGTGTCATTGG+GGG 0.701339 5:+85300893 MsG0580028716.01.T01:CDS
GGACACTCGACACATGACAG+AGG 0.703088 5:-85301180 None:intergenic
ATATCTCTCATCTTATCACG+GGG 0.716109 5:+85300301 MsG0580028716.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATTTTGAAATCTTAAATTT+CGG + Chr5:85300436-85300455 MsG0580028716.01.T01:CDS 15.0%
!! AATATAACTCAACAAAAACA+TGG - Chr5:85301651-85301670 None:intergenic 20.0%
!!! CGAAATTTAAGATTTCAAAA+TGG - Chr5:85300438-85300457 None:intergenic 20.0%
! AATAAATCCATCTATGTCTA+TGG - Chr5:85301007-85301026 None:intergenic 25.0%
! AATTAACCGAATTATGGATT+TGG + Chr5:85302515-85302534 MsG0580028716.01.T01:CDS 25.0%
! ATAAATCCATCTATGTCTAT+GGG - Chr5:85301006-85301025 None:intergenic 25.0%
! ATAGTTTAAGAAGTAACTCT+TGG + Chr5:85300815-85300834 MsG0580028716.01.T01:CDS 25.0%
! ATATATCTCTCATCTTATCA+CGG + Chr5:85300299-85300318 MsG0580028716.01.T01:CDS 25.0%
! ATGTAGACAATAAGTATTCA+TGG + Chr5:85301028-85301047 MsG0580028716.01.T01:CDS 25.0%
! ATTACAATCATGTGTATGAA+TGG + Chr5:85301789-85301808 MsG0580028716.01.T01:intron 25.0%
! CGAAATTAAACATAAAAGCA+TGG - Chr5:85301510-85301529 None:intergenic 25.0%
! TACTTATTTATTCCACCAAT+TGG - Chr5:85301357-85301376 None:intergenic 25.0%
! TGGATGTGGTAAATTTAATT+TGG - Chr5:85300389-85300408 None:intergenic 25.0%
! TGTCAGATAAGAAATTGTTA+TGG - Chr5:85300273-85300292 None:intergenic 25.0%
! TTAAAGGAAAATATGAGTGT+GGG + Chr5:85302437-85302456 MsG0580028716.01.T01:CDS 25.0%
!! CTCTAAAAGAGAATCAAATT+TGG + Chr5:85300936-85300955 MsG0580028716.01.T01:CDS 25.0%
!! TAACCTGATCTAAAACAAAT+TGG - Chr5:85302105-85302124 None:intergenic 25.0%
!! TTGTTTGTTGAAATTAGCTT+GGG + Chr5:85302375-85302394 MsG0580028716.01.T01:CDS 25.0%
!!! TTTCCAATTTGTTTTAGATC+AGG + Chr5:85302099-85302118 MsG0580028716.01.T01:CDS 25.0%
AAACTATATATCTCCCAGAA+GGG - Chr5:85300802-85300821 None:intergenic 30.0%
AATTAACTGTGAAGGAATCA+TGG + Chr5:85301136-85301155 MsG0580028716.01.T01:CDS 30.0%
AATTGGAAATCTGATTTACG+AGG - Chr5:85302088-85302107 None:intergenic 30.0%
ACAATGGAAAGAATGATCTT+TGG + Chr5:85302404-85302423 MsG0580028716.01.T01:CDS 30.0%
ACATTGCATACAAACTGATA+TGG + Chr5:85301430-85301449 MsG0580028716.01.T01:intron 30.0%
ATAAGATGAGAGATATATCG+TGG - Chr5:85300297-85300316 None:intergenic 30.0%
ATTCTGTGTCTCTATTTCAA+TGG + Chr5:85301727-85301746 MsG0580028716.01.T01:intron 30.0%
CCAAATGAATAAACTGAATG+TGG + Chr5:85301560-85301579 MsG0580028716.01.T01:intron 30.0%
CCACATTCAGTTTATTCATT+TGG - Chr5:85301563-85301582 None:intergenic 30.0%
CTAATTTCAACAAACAAGCT+TGG - Chr5:85302372-85302391 None:intergenic 30.0%
CTTAAAGGAAAATATGAGTG+TGG + Chr5:85302436-85302455 MsG0580028716.01.T01:CDS 30.0%
GACAAGTTATTAGTTACAGT+TGG - Chr5:85301905-85301924 None:intergenic 30.0%
GATCACATATCAAGAACAAA+TGG + Chr5:85301086-85301105 MsG0580028716.01.T01:CDS 30.0%
GGAAAGAATTAACCGAATTA+TGG + Chr5:85302509-85302528 MsG0580028716.01.T01:CDS 30.0%
GTTTCATGATGAAGGTTATT+GGG + Chr5:85301402-85301421 MsG0580028716.01.T01:intron 30.0%
TAAACTATATATCTCCCAGA+AGG - Chr5:85300803-85300822 None:intergenic 30.0%
TATATCTCTCATCTTATCAC+GGG + Chr5:85300300-85300319 MsG0580028716.01.T01:CDS 30.0%
TATGAAAGATACGCAAATAG+CGG - Chr5:85301830-85301849 None:intergenic 30.0%
TGATACAGATCATATTCGTA+AGG + Chr5:85302267-85302286 MsG0580028716.01.T01:CDS 30.0%
TTAACGAAAAACTTACTGCT+TGG + Chr5:85302122-85302141 MsG0580028716.01.T01:CDS 30.0%
TTGTTCTTGATATGTGATCA+AGG - Chr5:85301085-85301104 None:intergenic 30.0%
TTTAAGTACCCAAATGATTG+AGG + Chr5:85301266-85301285 MsG0580028716.01.T01:CDS 30.0%
TTTGAGTGAATTAACTGTGA+AGG + Chr5:85301128-85301147 MsG0580028716.01.T01:CDS 30.0%
! AAAGAATGATCTTTGGTTCA+AGG + Chr5:85302411-85302430 MsG0580028716.01.T01:CDS 30.0%
! CTTGTTTGTTGAAATTAGCT+TGG + Chr5:85302374-85302393 MsG0580028716.01.T01:CDS 30.0%
! TATTTCCTCTTTTAACACCA+AGG - Chr5:85301293-85301312 None:intergenic 30.0%
! TCAATGGTATACAGTTCTAA+TGG + Chr5:85301743-85301762 MsG0580028716.01.T01:intron 30.0%
! TGTTTGTTGAAATTAGCTTG+GGG + Chr5:85302376-85302395 MsG0580028716.01.T01:CDS 30.0%
!! CTTTAAGGTGATTAGTTATG+TGG + Chr5:85300687-85300706 MsG0580028716.01.T01:CDS 30.0%
AAAAAGATGAAGAGCTAGCT+TGG + Chr5:85301238-85301257 MsG0580028716.01.T01:CDS 35.0%
AAATTGAGAGAGCCTTTATC+TGG + Chr5:85302242-85302261 MsG0580028716.01.T01:CDS 35.0%
AAGGAAAACCTTCTTCCAAT+TGG + Chr5:85301339-85301358 MsG0580028716.01.T01:CDS 35.0%
AATTGAGAGAGCCTTTATCT+GGG + Chr5:85302243-85302262 MsG0580028716.01.T01:CDS 35.0%
ATACATCAATAGAACGCTTC+TGG + Chr5:85300761-85300780 MsG0580028716.01.T01:CDS 35.0%
ATATCTCTCATCTTATCACG+GGG + Chr5:85300301-85300320 MsG0580028716.01.T01:CDS 35.0%
ATCTCTTCCAAACAACTCTT+TGG - Chr5:85302217-85302236 None:intergenic 35.0%
GCGAAACCAAATCCATAATT+CGG - Chr5:85302524-85302543 None:intergenic 35.0%
GGATCAAACTCTTCATTGTT+GGG + Chr5:85301157-85301176 MsG0580028716.01.T01:CDS 35.0%
GGTTAATTCTTTCCAAAGTC+TGG - Chr5:85302503-85302522 None:intergenic 35.0%
GGTTTCATGATGAAGGTTAT+TGG + Chr5:85301401-85301420 MsG0580028716.01.T01:intron 35.0%
GTTAAAAGAGGAAATAGTCG+TGG + Chr5:85301297-85301316 MsG0580028716.01.T01:CDS 35.0%
GTTTAAGAAGTAACTCTTGG+AGG + Chr5:85300818-85300837 MsG0580028716.01.T01:CDS 35.0%
GTTTCAGTTCCTTCTTGAAA+TGG - Chr5:85300409-85300428 None:intergenic 35.0%
TAAGTATTCATGGAGAGACT+TGG + Chr5:85301038-85301057 MsG0580028716.01.T01:CDS 35.0%
TACATCAATAGAACGCTTCT+GGG + Chr5:85300762-85300781 MsG0580028716.01.T01:CDS 35.0%
TACATTCTCAAATCTTTCGC+CGG - Chr5:85300365-85300384 None:intergenic 35.0%
TGGATCAAACTCTTCATTGT+TGG + Chr5:85301156-85301175 MsG0580028716.01.T01:CDS 35.0%
TGGCTTTCTTTATAATAGCG+AGG - Chr5:85301631-85301650 None:intergenic 35.0%
TTACAGTTGGCAAATGATGT+GGG - Chr5:85301892-85301911 None:intergenic 35.0%
TTACCACATCCATTTCAAGA+AGG + Chr5:85300397-85300416 MsG0580028716.01.T01:CDS 35.0%
TTATTCCACCAATTGGAAGA+AGG - Chr5:85301350-85301369 None:intergenic 35.0%
TTGATATGTGATCAAGGCAA+TGG - Chr5:85301079-85301098 None:intergenic 35.0%
TTGTGAGATAGTAGTGTACA+AGG + Chr5:85301320-85301339 MsG0580028716.01.T01:CDS 35.0%
TTGTTATGCCAACAACATGT+GGG + Chr5:85300851-85300870 MsG0580028716.01.T01:CDS 35.0%
! ACCGGCAAAAGATAGATTTT+TGG - Chr5:85302152-85302171 None:intergenic 35.0%
! GCCAAAAATCTATCTTTTGC+CGG + Chr5:85302148-85302167 MsG0580028716.01.T01:CDS 35.0%
! TGTTTTCCAAAAATGTGTCG+CGG + Chr5:85301972-85301991 MsG0580028716.01.T01:intron 35.0%
! TTTTGTCTCGTGTAATCTCA+GGG + Chr5:85301676-85301695 MsG0580028716.01.T01:intron 35.0%
! TTTTTGTCTCGTGTAATCTC+AGG + Chr5:85301675-85301694 MsG0580028716.01.T01:intron 35.0%
!! GGAGTTTTGTATTCTTTGTC+CGG + Chr5:85300343-85300362 MsG0580028716.01.T01:CDS 35.0%
AACAACATGTGGGAATACGA+TGG + Chr5:85300861-85300880 MsG0580028716.01.T01:CDS 40.0%
AACCTACAAACTCCAGACTT+TGG + Chr5:85302488-85302507 MsG0580028716.01.T01:CDS 40.0%
AATCCATGAACTTGACGACT+TGG - Chr5:85300643-85300662 None:intergenic 40.0%
AATGATGTGGGCGAAATTCA+TGG - Chr5:85301880-85301899 None:intergenic 40.0%
ACAACATGTGGGAATACGAT+GGG + Chr5:85300862-85300881 MsG0580028716.01.T01:CDS 40.0%
ACCAAGGTCCTCAATCATTT+GGG - Chr5:85301277-85301296 None:intergenic 40.0%
ACTTTGAATTCGTCCGTAGT+TGG - Chr5:85300550-85300569 None:intergenic 40.0%
AGGTATCATTAGAACCCTTC+TGG + Chr5:85300785-85300804 MsG0580028716.01.T01:CDS 40.0%
AGTCGTCAAGTTCATGGATT+TGG + Chr5:85300643-85300662 MsG0580028716.01.T01:CDS 40.0%
ATCCATGAACTTGACGACTT+GGG - Chr5:85300642-85300661 None:intergenic 40.0%
ATCGTATTCCCACATGTTGT+TGG - Chr5:85300862-85300881 None:intergenic 40.0%
ATCTGTATCACCCCAGATAA+AGG - Chr5:85302257-85302276 None:intergenic 40.0%
ATTACGAGACCTGAAGATGT+TGG + Chr5:85302313-85302332 MsG0580028716.01.T01:CDS 40.0%
ATTGAGAGAGCCTTTATCTG+GGG + Chr5:85302244-85302263 MsG0580028716.01.T01:CDS 40.0%
CAAACAACTCTTTGGCATCT+TGG - Chr5:85302209-85302228 None:intergenic 40.0%
CAAAGTCATTCCTCATAGTC+CGG + Chr5:85300564-85300583 MsG0580028716.01.T01:CDS 40.0%
CAAGATGCCAAAGAGTTGTT+TGG + Chr5:85302207-85302226 MsG0580028716.01.T01:CDS 40.0%
CATAGTGCAAATCTTACAGC+AGG - Chr5:85300523-85300542 None:intergenic 40.0%
CCATCTATGTCTATGGGTAT+GGG - Chr5:85301000-85301019 None:intergenic 40.0%
CCATTAACATTTGCAGCGAA+CGG - Chr5:85300601-85300620 None:intergenic 40.0%
CCTGCGTACATATGACTTTA+AGG + Chr5:85300672-85300691 MsG0580028716.01.T01:CDS 40.0%
CCTTAAAGTCATATGTACGC+AGG - Chr5:85300675-85300694 None:intergenic 40.0%
CTGCTGTAAGATTTGCACTA+TGG + Chr5:85300521-85300540 MsG0580028716.01.T01:CDS 40.0%
GAAAACCTTCTTCCAATTGG+TGG + Chr5:85301342-85301361 MsG0580028716.01.T01:intron 40.0%
GGTATCATTAGAACCCTTCT+GGG + Chr5:85300786-85300805 MsG0580028716.01.T01:CDS 40.0%
GTTACAGTTGGCAAATGATG+TGG - Chr5:85301893-85301912 None:intergenic 40.0%
GTTCCTTCTTGAAATGGATG+TGG - Chr5:85300403-85300422 None:intergenic 40.0%
GTTGTTATGCCAACAACATG+TGG + Chr5:85300850-85300869 MsG0580028716.01.T01:CDS 40.0%
TAAGGTACATGCAGTTCGTT+GGG + Chr5:85302285-85302304 MsG0580028716.01.T01:CDS 40.0%
TCCATCTATGTCTATGGGTA+TGG - Chr5:85301001-85301020 None:intergenic 40.0%
TGAAACATATCCGGACTATG+AGG - Chr5:85300577-85300596 None:intergenic 40.0%
TGCACTATGGAATCCAACTA+CGG + Chr5:85300534-85300553 MsG0580028716.01.T01:CDS 40.0%
! CTTTGGTTCAAGGTGCTTAA+AGG + Chr5:85302421-85302440 MsG0580028716.01.T01:CDS 40.0%
! GTTTTCCAAAAATGTGTCGC+GGG + Chr5:85301973-85301992 MsG0580028716.01.T01:intron 40.0%
! TGTCATTGGGGGTGTATTAT+AGG + Chr5:85300904-85300923 MsG0580028716.01.T01:CDS 40.0%
! TTAGCTTGGGGTTTGTACAA+TGG + Chr5:85302388-85302407 MsG0580028716.01.T01:CDS 40.0%
! TTCCAAAGTCTGGAGTTTGT+AGG - Chr5:85302493-85302512 None:intergenic 40.0%
!! ACTTGAATGGACTGTGTCAT+TGG + Chr5:85300890-85300909 MsG0580028716.01.T01:CDS 40.0%
!! CTTGAATGGACTGTGTCATT+GGG + Chr5:85300891-85300910 MsG0580028716.01.T01:CDS 40.0%
!! TTGAATGGACTGTGTCATTG+GGG + Chr5:85300892-85300911 MsG0580028716.01.T01:CDS 40.0%
AAAACTCCCACCCATATGTC+AGG - Chr5:85300331-85300350 None:intergenic 45.0%
AAGCTTGGTTCATGACAGTG+AGG - Chr5:85302357-85302376 None:intergenic 45.0%
AATGCCACATAACCTTTGCG+TGG - Chr5:85300736-85300755 None:intergenic 45.0%
ACCCAAATGATTGAGGACCT+TGG + Chr5:85301273-85301292 MsG0580028716.01.T01:CDS 45.0%
ATCAATAGAACGCTTCTGGG+AGG + Chr5:85300765-85300784 MsG0580028716.01.T01:CDS 45.0%
CACCAAGGTCCTCAATCATT+TGG - Chr5:85301278-85301297 None:intergenic 45.0%
CCCATACCCATAGACATAGA+TGG + Chr5:85300997-85301016 MsG0580028716.01.T01:CDS 45.0%
CCGTTCGCTGCAAATGTTAA+TGG + Chr5:85300598-85300617 MsG0580028716.01.T01:CDS 45.0%
CCTCTAGCGAACACAAGAAA+AGG - Chr5:85301383-85301402 None:intergenic 45.0%
CGCAAAGGTTATGTGGCATT+AGG + Chr5:85300736-85300755 MsG0580028716.01.T01:CDS 45.0%
CGCTAGAGGTTTCATGATGA+AGG + Chr5:85301394-85301413 MsG0580028716.01.T01:intron 45.0%
CTTTGCGTGGATTCAATCGT+GGG - Chr5:85300723-85300742 None:intergenic 45.0%
GAATCCACGCAAAGGTTATG+TGG + Chr5:85300729-85300748 MsG0580028716.01.T01:CDS 45.0%
GCGAACGGTTGAAACATATC+CGG - Chr5:85300586-85300605 None:intergenic 45.0%
GTAAGGTACATGCAGTTCGT+TGG + Chr5:85302284-85302303 MsG0580028716.01.T01:CDS 45.0%
GTATTCATGGAGAGACTTGG+CGG + Chr5:85301041-85301060 MsG0580028716.01.T01:CDS 45.0%
TATGTCTATGGGTATGGGTG+TGG - Chr5:85300995-85301014 None:intergenic 45.0%
TTTGCGTGGATTCAATCGTG+GGG - Chr5:85300722-85300741 None:intergenic 45.0%
! ACGATGGGAGTGTACTTGAA+TGG + Chr5:85300877-85300896 MsG0580028716.01.T01:CDS 45.0%
! CCTTTTCTTGTGTTCGCTAG+AGG + Chr5:85301380-85301399 MsG0580028716.01.T01:intron 45.0%
! TCATGTGTCGAGTGTCCTTT+TGG + Chr5:85301186-85301205 MsG0580028716.01.T01:CDS 45.0%
! TTAGAGAAGCTGTGTCGCTA+TGG + Chr5:85302029-85302048 MsG0580028716.01.T01:CDS 45.0%
! TTTTGCTAGAGTCACTCGAC+CGG - Chr5:85302170-85302189 None:intergenic 45.0%
!! GAGGACCTTGGTGTTAAAAG+AGG + Chr5:85301285-85301304 MsG0580028716.01.T01:CDS 45.0%
!! GTGTCGCTATGGAAGTATCT+TGG + Chr5:85302040-85302059 MsG0580028716.01.T01:CDS 45.0%
!! TGAATGGACTGTGTCATTGG+GGG + Chr5:85300893-85300912 MsG0580028716.01.T01:CDS 45.0%
!!! TGTCCTTTTGGAGTGGGAAA+GGG + Chr5:85301198-85301217 MsG0580028716.01.T01:CDS 45.0%
AAAATGTGTCGCGGGAGATG+AGG + Chr5:85301981-85302000 MsG0580028716.01.T01:intron 50.0%
ACGAGACCTGAAGATGTTGG+CGG + Chr5:85302316-85302335 MsG0580028716.01.T01:CDS 50.0%
ACGGGGTAGACCTGACATAT+GGG + Chr5:85300318-85300337 MsG0580028716.01.T01:CDS 50.0%
CACCCAAGTCGTCAAGTTCA+TGG + Chr5:85300637-85300656 MsG0580028716.01.T01:CDS 50.0%
CCACGATTGAATCCACGCAA+AGG + Chr5:85300721-85300740 MsG0580028716.01.T01:CDS 50.0%
CCTTTGCGTGGATTCAATCG+TGG - Chr5:85300724-85300743 None:intergenic 50.0%
GGTAGACCTGACATATGGGT+GGG + Chr5:85300322-85300341 MsG0580028716.01.T01:CDS 50.0%
GTCTCGTGTAATCTCAGGGT+TGG + Chr5:85301680-85301699 MsG0580028716.01.T01:intron 50.0%
TTGCCCTTTCCCACTCCAAA+AGG - Chr5:85301204-85301223 None:intergenic 50.0%
! CATCTCCCGCGACACATTTT+TGG - Chr5:85301981-85302000 None:intergenic 50.0%
!! TGTCGAGTGTCCTTTTGGAG+TGG + Chr5:85301191-85301210 MsG0580028716.01.T01:CDS 50.0%
!!! GTCGAGTGTCCTTTTGGAGT+GGG + Chr5:85301192-85301211 MsG0580028716.01.T01:CDS 50.0%
!!! GTGTCCTTTTGGAGTGGGAA+AGG + Chr5:85301197-85301216 MsG0580028716.01.T01:CDS 50.0%
CACGGGGTAGACCTGACATA+TGG + Chr5:85300317-85300336 MsG0580028716.01.T01:CDS 55.0%
CGAGGCGCAATCTCAGTCAA+TGG - Chr5:85302070-85302089 None:intergenic 55.0%
GCGGGAGATGAGGAATCGAT+TGG + Chr5:85301991-85302010 MsG0580028716.01.T01:intron 55.0%
GGACACTCGACACATGACAG+AGG - Chr5:85301183-85301202 None:intergenic 55.0%
GGGTAGACCTGACATATGGG+TGG + Chr5:85300321-85300340 MsG0580028716.01.T01:CDS 55.0%
! ACCTGAAGATGTTGGCGGAC+TGG + Chr5:85302321-85302340 MsG0580028716.01.T01:CDS 55.0%
!! CCTGAAGATGTTGGCGGACT+GGG + Chr5:85302322-85302341 MsG0580028716.01.T01:CDS 55.0%
CCCAGTCCGCCAACATCTTC+AGG - Chr5:85302325-85302344 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 85300265 85302594 85300265 ID=MsG0580028716.01;Name=MsG0580028716.01
Chr5 mRNA 85300265 85302594 85300265 ID=MsG0580028716.01.T01;Parent=MsG0580028716.01;Name=MsG0580028716.01.T01;_AED=0.57;_eAED=0.57;_QI=0|0|0|0.5|1|1|2|0|554
Chr5 exon 85300265 85301360 85300265 ID=MsG0580028716.01.T01:exon:29885;Parent=MsG0580028716.01.T01
Chr5 exon 85302026 85302594 85302026 ID=MsG0580028716.01.T01:exon:29886;Parent=MsG0580028716.01.T01
Chr5 CDS 85300265 85301360 85300265 ID=MsG0580028716.01.T01:cds;Parent=MsG0580028716.01.T01
Chr5 CDS 85302026 85302594 85302026 ID=MsG0580028716.01.T01:cds;Parent=MsG0580028716.01.T01
Gene Sequence

>MsG0580028716.01.T01

ATGGTCCATAACAATTTCTTATCTGACAACCACGATATATCTCTCATCTTATCACGGGGTAGACCTGACATATGGGTGGGAGTTTTGTATTCTTTGTCCGGCGAAAGATTTGAGAATGTAGCCAAATTAAATTTACCACATCCATTTCAAGAAGGAACTGAAACTCTCAACCATTTTGAAATCTTAAATTTCGGCAGTATTTATGACGATTTTATTTTTGTCAAATGTTTTCTTAAACATAATGTTAACGTCAATCCTGCTGTAAGATTTGCACTATGGAATCCAACTACGGACGAATTCAAAGTCATTCCTCATAGTCCGGATATGTTTCAACCGTTCGCTGCAAATGTTAATGGTGATGTGATTAATTTTCACCCAAGTCGTCAAGTTCATGGATTTGGCTACGACCTGCGTACATATGACTTTAAGGTGATTAGTTATGTGGAGTTTAGTGCCCCACGATTGAATCCACGCAAAGGTTATGTGGCATTAGGTGATACATCAATAGAACGCTTCTGGGAGGTATCATTAGAACCCTTCTGGGAGATATATAGTTTAAGAAGTAACTCTTGGAGGAAACTTGATGTTGTTATGCCAACAACATGTGGGAATACGATGGGAGTGTACTTGAATGGACTGTGTCATTGGGGGTGTATTATAGGTCATTTTGACTCTAAAAGAGAATCAAATTTGGTGTCGTTTGATCTGAGCAATGATGTGTTCTTTACCACACCCATACCCATAGACATAGATGGATTTATTAATGTAGACAATAAGTATTCATGGAGAGACTTGGCGGTGTTAAATGAATCCATTGCCTTGATCACATATCAAGAACAAATGGCTACTTTTAACATATCAATTTTGAGTGAATTAACTGTGAAGGAATCATGGATCAAACTCTTCATTGTTGGGCCTCTGTCATGTGTCGAGTGTCCTTTTGGAGTGGGAAAGGGCAAAATATTCTTCACAAAAAAAGATGAAGAGCTAGCTTGGTTTGATTTAAGTACCCAAATGATTGAGGACCTTGGTGTTAAAAGAGGAAATAGTCGTGGTTGTGAGATAGTAGTGTACAAGGAAAACCTTCTTCCAATTGACTTTAGAGAAGCTGTGTCGCTATGGAAGTATCTTGGTGTTCCATTGACTGAGATTGCGCCTCGTAAATCAGATTTCCAATTTGTTTTAGATCAGGTTAACGAAAAACTTACTGCTTGGAAAGCCAAAAATCTATCTTTTGCCGGTCGAGTGACTCTAGCAAAAAGTTATATGATGACTGCCAAGATGCCAAAGAGTTGTTTGGAAGAGATTGAAAAAATTGAGAGAGCCTTTATCTGGGGTGATACAGATCATATTCGTAAGGTACATGCAGTTCGTTGGGAAGTTATTACGAGACCTGAAGATGTTGGCGGACTGGGATTGAGAAACCTCACTGTCATGAACCAAGCTTGTTTGTTGAAATTAGCTTGGGGTTTGTACAATGGAAAGAATGATCTTTGGTTCAAGGTGCTTAAAGGAAAATATGAGTGTGGGCATCAGCTTGATAATAATGTTGTTGTCAAACCTACAAACTCCAGACTTTGGAAAGAATTAACCGAATTATGGATTTGGTTTCGCTTTAATCAATGTTTTATGCATTCATTGCAGTCAGCAAATTTGAACTACTAG
Protein sequence

>MsG0580028716.01.T01

MVHNNFLSDNHDISLILSRGRPDIWVGVLYSLSGERFENVAKLNLPHPFQEGTETLNHFEILNFGSIYDDFIFVKCFLKHNVNVNPAVRFALWNPTTDEFKVIPHSPDMFQPFAANVNGDVINFHPSRQVHGFGYDLRTYDFKVISYVEFSAPRLNPRKGYVALGDTSIERFWEVSLEPFWEIYSLRSNSWRKLDVVMPTTCGNTMGVYLNGLCHWGCIIGHFDSKRESNLVSFDLSNDVFFTTPIPIDIDGFINVDNKYSWRDLAVLNESIALITYQEQMATFNISILSELTVKESWIKLFIVGPLSCVECPFGVGKGKIFFTKKDEELAWFDLSTQMIEDLGVKRGNSRGCEIVVYKENLLPIDFREAVSLWKYLGVPLTEIAPRKSDFQFVLDQVNEKLTAWKAKNLSFAGRVTLAKSYMMTAKMPKSCLEEIEKIERAFIWGDTDHIRKVHAVRWEVITRPEDVGGLGLRNLTVMNQACLLKLAWGLYNGKNDLWFKVLKGKYECGHQLDNNVVVKPTNSRLWKELTELWIWFRFNQCFMHSLQSANLNY*