AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580030117.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580030117.01.T01 MTR_5g037680 62.242 339 104 7 1 320 47 380 3.52e-140 404
MsG0580030117.01.T01 MTR_5g059430 57.025 363 120 8 1 327 65 427 7.86e-137 395
MsG0580030117.01.T01 MTR_5g069390 58.028 355 118 8 1 326 65 417 1.98e-136 397
MsG0580030117.01.T01 MTR_5g097820 58.491 212 71 4 1 199 62 269 8.95e-82 249
MsG0580030117.01.T01 MTR_5g097350 44.345 336 168 10 1 323 55 384 3.31e-81 251
MsG0580030117.01.T01 MTR_5g097330 44.258 357 160 11 1 326 55 403 3.35e-81 252
MsG0580030117.01.T01 MTR_6g086825 42.975 363 159 11 1 326 109 460 8.07e-79 248
MsG0580030117.01.T01 MTR_7g111700 42.286 350 147 9 5 326 43 365 9.20e-78 242
MsG0580030117.01.T01 MTR_8g012090 45.079 315 119 9 1 296 55 334 2.14e-76 238
MsG0580030117.01.T01 MTR_7g017040 43.875 351 153 11 1 320 55 392 1.81e-75 237
MsG0580030117.01.T01 MTR_8g063440 43.323 337 164 10 1 320 51 377 7.16e-75 235
MsG0580030117.01.T01 MTR_5g096910 44.409 313 155 10 30 324 80 391 3.42e-73 231
MsG0580030117.01.T01 MTR_7g017130 42.000 350 170 10 1 326 57 397 1.60e-72 229
MsG0580030117.01.T01 MTR_2g038680 42.033 364 162 11 1 324 55 409 2.52e-72 229
MsG0580030117.01.T01 MTR_0054s0090 42.779 367 149 13 1 324 56 404 6.67e-72 228
MsG0580030117.01.T01 MTR_5g096630 41.096 365 169 13 1 326 57 414 1.74e-71 228
MsG0580030117.01.T01 MTR_7g017170 40.884 362 169 11 1 326 57 409 2.07e-71 227
MsG0580030117.01.T01 MTR_0054s0100 42.120 368 152 13 1 324 56 406 6.03e-71 226
MsG0580030117.01.T01 MTR_2g104340 41.944 360 161 13 2 326 50 396 9.03e-70 223
MsG0580030117.01.T01 MTR_5g097220 39.189 370 162 12 1 323 52 405 1.58e-69 222
MsG0580030117.01.T01 MTR_4g102410 43.137 357 162 15 1 324 100 448 1.69e-69 223
MsG0580030117.01.T01 MTR_0129s0070 40.741 351 173 11 1 326 56 396 9.38e-69 220
MsG0580030117.01.T01 MTR_6g080680 42.090 354 151 12 1 321 58 390 3.71e-68 218
MsG0580030117.01.T01 MTR_8g041270 40.331 362 173 10 2 326 51 406 7.04e-68 218
MsG0580030117.01.T01 MTR_0129s0120 41.092 348 170 11 1 323 56 393 2.06e-67 216
MsG0580030117.01.T01 MTR_2g022000 40.483 373 171 11 1 326 55 423 3.66e-67 217
MsG0580030117.01.T01 MTR_6g478230 39.227 362 164 11 1 326 66 407 1.87e-65 212
MsG0580030117.01.T01 MTR_2g021800 41.783 359 165 12 1 319 55 409 3.79e-65 211
MsG0580030117.01.T01 MTR_7g069550 39.943 348 170 11 1 318 53 391 8.22e-65 210
MsG0580030117.01.T01 MTR_5g074800 40.571 350 162 13 1 318 1 336 2.01e-64 207
MsG0580030117.01.T01 MTR_4g130330 38.827 358 167 13 5 324 46 389 2.09e-64 209
MsG0580030117.01.T01 MTR_8g464770 40.060 332 175 10 5 322 55 376 3.94e-63 205
MsG0580030117.01.T01 MTR_6g090180 39.385 358 174 12 1 323 86 435 5.22e-63 206
MsG0580030117.01.T01 MTR_6g078010 41.618 346 157 14 1 320 3 329 6.47e-63 203
MsG0580030117.01.T01 MTR_4g028510 39.889 361 170 15 1 327 56 403 2.95e-62 205
MsG0580030117.01.T01 MTR_3g011120 40.419 334 161 14 2 315 54 369 3.08e-62 202
MsG0580030117.01.T01 MTR_4g007940 40.176 341 162 13 12 319 4 335 4.64e-62 201
MsG0580030117.01.T01 MTR_6g079770 40.053 377 160 17 1 326 56 417 6.87e-62 203
MsG0580030117.01.T01 MTR_8g033140 38.196 377 166 13 1 326 58 418 8.44e-62 202
MsG0580030117.01.T01 MTR_2g104280 39.071 366 172 12 2 326 50 405 1.06e-61 202
MsG0580030117.01.T01 MTR_0831s0010 39.080 348 163 12 1 320 89 415 3.60e-61 201
MsG0580030117.01.T01 MTR_6g079810 39.554 359 162 12 11 326 117 463 4.19e-61 202
MsG0580030117.01.T01 MTR_6g461660 39.776 357 170 14 1 326 58 400 8.74e-61 199
MsG0580030117.01.T01 MTR_1g007410 41.641 329 157 10 1 302 61 381 2.20e-60 198
MsG0580030117.01.T01 MTR_5g086590 43.933 239 106 6 88 309 11 238 1.09e-59 191
MsG0580030117.01.T01 MTR_0129s0050 38.814 371 167 13 1 326 53 408 1.55e-59 196
MsG0580030117.01.T01 MTR_4g028660 38.592 355 173 13 1 323 55 396 1.98e-59 196
MsG0580030117.01.T01 MTR_4g027450 38.592 355 173 13 1 323 55 396 1.98e-59 196
MsG0580030117.01.T01 MTR_5g064700 37.234 376 180 13 1 327 1 369 2.38e-59 195
MsG0580030117.01.T01 MTR_5g097850 43.431 274 98 7 68 324 10 243 3.16e-59 191
MsG0580030117.01.T01 MTR_7g079640 41.195 318 157 10 1 304 52 353 8.35e-59 194
MsG0580030117.01.T01 MTR_4g028680 39.326 356 172 13 1 324 55 398 6.71e-58 192
MsG0580030117.01.T01 MTR_4g055820 39.062 320 139 12 2 281 54 357 1.15e-57 190
MsG0580030117.01.T01 MTR_2g066200 36.888 347 185 13 1 324 51 386 1.18e-57 191
MsG0580030117.01.T01 MTR_4g117550 38.040 347 165 13 1 326 56 373 1.62e-57 190
MsG0580030117.01.T01 MTR_2g021770 37.845 362 184 13 1 326 51 407 1.82e-57 191
MsG0580030117.01.T01 MTR_2g021950 37.249 349 178 12 1 320 55 391 1.30e-56 188
MsG0580030117.01.T01 MTR_6g079790 39.873 316 144 11 50 326 1 309 1.61e-56 186
MsG0580030117.01.T01 MTR_6g087620 40.984 305 127 11 39 316 1 279 4.60e-56 184
MsG0580030117.01.T01 MTR_1g007450 40.719 334 157 13 1 304 51 373 5.78e-56 187
MsG0580030117.01.T01 MTR_5g092860 37.391 345 140 12 1 304 41 350 7.24e-56 186
MsG0580030117.01.T01 MTR_5g097680 36.939 379 179 11 1 326 56 427 9.91e-56 187
MsG0580030117.01.T01 MTR_0134s0060 37.772 368 167 13 2 320 52 406 1.54e-55 186
MsG0580030117.01.T01 MTR_5g022850 39.308 318 158 9 39 326 41 353 2.25e-55 184
MsG0580030117.01.T01 MTR_4g028750 40.303 330 152 14 5 303 59 374 2.88e-55 185
MsG0580030117.01.T01 MTR_8g013000 38.988 336 166 10 1 308 49 373 5.41e-55 184
MsG0580030117.01.T01 MTR_2g066170 37.644 348 179 15 1 324 51 384 7.38e-55 184
MsG0580030117.01.T01 MTR_6g090250 36.340 388 166 14 1 323 50 421 3.60e-54 183
MsG0580030117.01.T01 MTR_4g014370 35.150 367 186 10 1 324 50 407 4.36e-54 182
MsG0580030117.01.T01 MTR_7g016930 39.869 306 134 9 1 285 55 331 3.28e-53 178
MsG0580030117.01.T01 MTR_7g023800 39.233 339 159 12 1 323 1 308 5.25e-53 177
MsG0580030117.01.T01 MTR_8g046310 37.237 333 185 13 1 322 55 374 8.96e-53 178
MsG0580030117.01.T01 MTR_2g065860 36.311 347 187 12 1 324 51 386 1.07e-52 178
MsG0580030117.01.T01 MTR_8g039500 38.924 316 153 14 1 296 51 346 2.11e-52 176
MsG0580030117.01.T01 MTR_5g072060 39.339 333 141 12 1 302 45 347 9.39e-52 181
MsG0580030117.01.T01 MTR_5g023970 49.735 189 84 5 149 326 8 196 9.61e-52 170
MsG0580030117.01.T01 MTR_5g092850 37.466 363 163 15 1 320 55 396 2.57e-51 175
MsG0580030117.01.T01 MTR_2g064950 37.037 351 169 11 1 317 49 381 4.53e-51 174
MsG0580030117.01.T01 MTR_1g053510 36.986 365 171 15 1 322 54 402 8.07e-51 174
MsG0580030117.01.T01 MTR_5g043605 36.913 298 166 10 40 322 45 335 2.02e-50 171
MsG0580030117.01.T01 MTR_5g074850 37.861 346 153 17 1 304 76 401 5.00e-50 179
MsG0580030117.01.T01 MTR_8g063330 37.299 311 151 10 1 282 53 348 1.16e-49 172
MsG0580030117.01.T01 MTR_8g046200 35.106 376 175 14 5 322 54 418 1.63e-49 171
MsG0580030117.01.T01 MTR_5g097540 38.199 322 128 11 1 312 3 263 2.60e-49 167
MsG0580030117.01.T01 MTR_5g091140 38.710 341 149 13 1 309 87 399 4.91e-49 169
MsG0580030117.01.T01 MTR_2g065840 35.345 348 190 14 1 324 51 387 2.31e-48 167
MsG0580030117.01.T01 MTR_1g007430 39.228 311 142 14 1 285 35 324 6.84e-48 164
MsG0580030117.01.T01 MTR_1g050312 39.590 293 134 11 29 304 6 272 8.16e-48 162
MsG0580030117.01.T01 MTR_5g075370 37.069 348 156 18 1 304 49 377 1.56e-47 171
MsG0580030117.01.T01 MTR_2g034700 36.873 339 180 13 1 323 53 373 2.33e-47 164
MsG0580030117.01.T01 MTR_5g096900 43.066 274 121 12 1 250 51 313 9.94e-47 160
MsG0580030117.01.T01 MTR_8g467670 34.431 334 193 13 5 325 57 377 2.90e-46 161
MsG0580030117.01.T01 MTR_5g066420 55.705 149 52 3 181 315 1 149 7.45e-46 153
MsG0580030117.01.T01 MTR_5g068490 37.722 281 154 13 48 322 8 273 7.82e-46 157
MsG0580030117.01.T01 MTR_6g090260 46.524 187 85 4 152 323 75 261 3.94e-45 155
MsG0580030117.01.T01 MTR_5g024260 33.819 343 179 14 1 309 61 389 6.18e-45 158
MsG0580030117.01.T01 MTR_8g036970 33.025 324 159 12 1 313 37 313 1.75e-44 156
MsG0580030117.01.T01 MTR_8g063350 36.644 292 141 14 38 301 44 319 2.05e-44 155
MsG0580030117.01.T01 MTR_8g033110 33.598 378 186 15 1 327 53 416 7.20e-44 155
MsG0580030117.01.T01 MTR_6g004450 45.000 220 99 7 124 324 51 267 1.83e-43 150
MsG0580030117.01.T01 MTR_5g076490 36.339 366 161 11 1 326 3 336 7.77e-43 151
MsG0580030117.01.T01 MTR_2g066150 34.503 342 176 14 1 326 51 360 8.21e-43 151
MsG0580030117.01.T01 MTR_8g046220 31.818 374 176 14 1 322 56 402 1.51e-42 152
MsG0580030117.01.T01 MTR_1g098180 31.897 348 176 10 1 326 58 366 1.89e-42 150
MsG0580030117.01.T01 MTR_8g064890 34.727 311 172 13 39 326 86 388 5.26e-42 150
MsG0580030117.01.T01 MTR_8g032980 32.642 386 181 12 1 326 55 421 6.73e-41 148
MsG0580030117.01.T01 MTR_8g033090 32.000 375 193 13 1 327 53 413 9.69e-41 147
MsG0580030117.01.T01 MTR_4g014370 32.938 337 151 9 1 324 61 335 2.73e-40 144
MsG0580030117.01.T01 MTR_8g063370 34.139 331 162 17 1 302 63 366 6.98e-40 144
MsG0580030117.01.T01 MTR_8g018110 32.787 366 187 13 1 327 53 398 9.84e-40 144
MsG0580030117.01.T01 MTR_4g016000 43.350 203 81 7 95 277 2 190 1.43e-38 136
MsG0580030117.01.T01 MTR_6g009350 43.386 189 82 7 124 290 10 195 1.94e-36 130
MsG0580030117.01.T01 MTR_8g064790 30.847 295 156 9 39 326 84 337 4.92e-36 133
MsG0580030117.01.T01 MTR_7g019250 40.000 220 103 5 104 305 206 414 1.09e-35 134
MsG0580030117.01.T01 MTR_3g018750 36.100 241 112 10 124 326 5 241 1.14e-35 129
MsG0580030117.01.T01 MTR_5g097660 60.000 115 44 2 215 327 7 121 1.88e-35 125
MsG0580030117.01.T01 MTR_5g097650 61.404 114 26 4 2 115 62 157 2.52e-35 126
MsG0580030117.01.T01 MTR_2g021920 39.485 233 101 9 124 320 39 267 2.84e-35 129
MsG0580030117.01.T01 MTR_1g050308 33.433 335 154 12 1 326 73 347 4.71e-35 130
MsG0580030117.01.T01 MTR_8g033000 42.051 195 98 5 147 327 171 364 5.64e-35 130
MsG0580030117.01.T01 MTR_5g098950 41.148 209 84 8 92 285 90 274 1.87e-34 127
MsG0580030117.01.T01 MTR_8g035690 36.531 271 118 11 101 324 38 301 2.51e-34 127
MsG0580030117.01.T01 MTR_7g104710 37.262 263 112 10 1 242 56 286 3.95e-34 127
MsG0580030117.01.T01 MTR_8g063310 28.653 349 192 14 6 318 62 389 3.96e-34 129
MsG0580030117.01.T01 MTR_5g096920 47.712 153 73 5 181 327 1 152 5.52e-34 122
MsG0580030117.01.T01 MTR_6g079730 50.746 134 61 3 195 323 14 147 5.08e-32 117
MsG0580030117.01.T01 MTR_5g091150 35.714 224 94 8 50 248 1 199 1.02e-30 115
MsG0580030117.01.T01 MTR_7g017150 40.000 195 106 3 143 326 73 267 2.60e-28 110
MsG0580030117.01.T01 MTR_7g017120 40.323 186 100 3 152 326 81 266 9.54e-28 109
MsG0580030117.01.T01 MTR_3g030620 42.945 163 77 5 177 324 19 180 1.30e-27 106
MsG0580030117.01.T01 MTR_2g104260 36.287 237 111 10 2 207 50 277 1.60e-27 108
MsG0580030117.01.T01 MTR_1g076810 41.243 177 86 7 1 168 57 224 3.27e-27 112
MsG0580030117.01.T01 MTR_7g101200 34.597 211 104 8 89 284 37 228 8.41e-26 103
MsG0580030117.01.T01 MTR_5g036250 47.619 105 55 0 223 327 8 112 1.43e-25 101
MsG0580030117.01.T01 MTR_5g037190 37.441 211 107 8 1 201 55 250 1.94e-25 102
MsG0580030117.01.T01 MTR_1g047080 27.647 340 169 14 1 324 3 281 7.79e-25 102
MsG0580030117.01.T01 MTR_5g049560 43.448 145 72 5 1 136 3 146 1.62e-24 100
MsG0580030117.01.T01 MTR_5g086780 32.331 266 100 9 46 303 35 228 3.72e-23 96.3
MsG0580030117.01.T01 MTR_5g096050 50.909 110 35 3 181 271 1 110 4.57e-23 92.8
MsG0580030117.01.T01 MTR_6g070880 34.848 198 88 7 1 169 71 256 8.52e-23 95.9
MsG0580030117.01.T01 MTR_6g071420 34.848 198 88 7 1 169 71 256 8.52e-23 95.9
MsG0580030117.01.T01 MTR_2g022020 48.544 103 52 1 218 320 13 114 1.94e-21 88.2
MsG0580030117.01.T01 MTR_5g094360 39.831 118 54 3 99 199 39 156 1.06e-18 82.0
MsG0580030117.01.T01 MTR_5g096070 47.619 84 42 2 48 131 79 160 3.06e-18 80.9
MsG0580030117.01.T01 MTR_5g094350 29.804 255 110 10 1 216 52 276 2.29e-17 81.3
MsG0580030117.01.T01 MTR_3g103020 25.606 289 186 11 56 326 98 375 5.37e-16 78.6
MsG0580030117.01.T01 MTR_5g097730 77.500 40 9 0 250 289 16 55 8.79e-16 71.2
MsG0580030117.01.T01 MTR_7g079370 26.380 326 196 13 33 322 32 349 3.56e-15 75.9
MsG0580030117.01.T01 MTR_1g050305 52.439 82 34 2 228 304 21 102 7.03e-15 71.2
MsG0580030117.01.T01 MTR_8g073570 34.586 133 76 5 1 133 49 170 1.65e-13 68.2
MsG0580030117.01.T01 MTR_4g094400 38.095 105 43 3 178 260 7 111 2.27e-13 66.6
MsG0580030117.01.T01 MTR_6g060650 26.772 254 163 10 89 326 124 370 6.02e-13 69.3
MsG0580030117.01.T01 MTR_2g078870 24.900 249 157 9 49 290 36 261 2.02e-12 67.4
MsG0580030117.01.T01 MTR_3g047540 47.619 63 30 2 152 211 6 68 3.95e-12 62.0
MsG0580030117.01.T01 MTR_2g028140 26.887 212 121 11 78 262 117 321 1.12e-11 65.9
MsG0580030117.01.T01 MTR_2g029990 26.792 265 168 11 9 262 89 338 1.88e-11 65.1
MsG0580030117.01.T01 MTR_0064s0160 26.471 238 148 8 92 304 165 400 2.96e-11 64.3
MsG0580030117.01.T01 MTR_2g078900 25.225 222 139 9 49 262 111 313 6.42e-11 63.2
MsG0580030117.01.T01 MTR_1g068835 23.140 242 161 11 75 299 91 324 6.53e-11 63.2
MsG0580030117.01.T01 MTR_4g117780 27.103 321 157 17 45 326 82 364 6.76e-11 63.2
MsG0580030117.01.T01 MTR_4g021785 25.979 281 165 12 66 326 95 352 7.21e-11 62.8
MsG0580030117.01.T01 MTR_1g097560 28.090 267 156 16 69 320 127 372 8.14e-11 62.8
MsG0580030117.01.T01 MTR_1g097330 25.391 256 150 12 75 305 109 348 8.37e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 65 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCAAGTTTCTCGATCTTC+TGG 0.208952 5:+106910226 None:intergenic
TGGTTTGACCATCGTGAATT+TGG 0.276325 5:-106910762 MsG0580030117.01.T01:CDS
CTATATATCTCCCACATATA+GGG 0.293868 5:+106910669 None:intergenic
GATTGGCCAGAGTATGATTC+TGG 0.299951 5:-106910933 MsG0580030117.01.T01:CDS
TAGGACCTAATTGGGGAGTT+TGG 0.321647 5:-106910782 MsG0580030117.01.T01:CDS
ACTATATATCTCCCACATAT+AGG 0.326320 5:+106910668 None:intergenic
TGGTGATAGATTTGAGAATA+TGG 0.331334 5:-106910985 MsG0580030117.01.T01:CDS
GGCCAAATGTCTTATTAGAT+TGG 0.336489 5:-106910950 MsG0580030117.01.T01:CDS
AGCTTAAATTCCATAGTAGT+TGG 0.355267 5:+106910828 None:intergenic
GTTATTGACGAAACTAATTT+CGG 0.364304 5:-106910693 MsG0580030117.01.T01:CDS
CCACAATATAATATTCTTAC+GGG 0.369410 5:+106910854 None:intergenic
GAAAGCCTTCTTCAATTTGA+GGG 0.390424 5:-106910165 MsG0580030117.01.T01:CDS
TCAAACCAAACTCCCCAATT+AGG 0.392362 5:+106910777 None:intergenic
TTATCGATGGACTCTCTCAT+TGG 0.402817 5:-106910575 MsG0580030117.01.T01:CDS
CTCTCTCATTGGATGTGTTA+TGG 0.405648 5:-106910564 MsG0580030117.01.T01:CDS
TCCACAATATAATATTCTTA+CGG 0.407485 5:+106910853 None:intergenic
TTTAGGTGCGCTTGGTGTAA+TGG 0.410117 5:-106910376 MsG0580030117.01.T01:CDS
CCTTGCCTTGATTATCGAAT+CGG 0.411290 5:-106910318 MsG0580030117.01.T01:CDS
AGAAAGCCTTCTTCAATTTG+AGG 0.412911 5:-106910166 MsG0580030117.01.T01:CDS
CATATTTGCTGTCATTTGAC+TGG 0.419300 5:-106910536 MsG0580030117.01.T01:CDS
GGCCAATCTAATAAGACATT+TGG 0.423433 5:+106910948 None:intergenic
AATAATTGTTGATCAATCAT+AGG 0.423502 5:+106910597 None:intergenic
TTGATGTCGATATGCATAAC+AGG 0.430857 5:-106910620 MsG0580030117.01.T01:CDS
CAATTAGGTCCTAAATCAAA+AGG 0.431969 5:+106910792 None:intergenic
ATAGTCTAAGAAGTAACTCT+TGG 0.438522 5:-106910650 MsG0580030117.01.T01:CDS
TTTGAGTTGTATTCTATTTG+TGG 0.439612 5:-106911005 MsG0580030117.01.T01:CDS
GCCAAGTTTCTCGATCTTCT+GGG 0.449802 5:+106910227 None:intergenic
ATTGTGGAATCCAACTACTA+TGG 0.463872 5:-106910838 MsG0580030117.01.T01:CDS
CCACTCCGATTCGATAATCA+AGG 0.479939 5:+106910313 None:intergenic
TGGACTAAAGTCTTCATAGT+AGG 0.486691 5:-106910348 MsG0580030117.01.T01:CDS
TTGCGCCCTCAAATTGAAGA+AGG 0.490082 5:+106910160 None:intergenic
CGCTTGGTGTAATGGAATCA+TGG 0.495546 5:-106910368 MsG0580030117.01.T01:CDS
ATATTCAGTGTGGAAACAAT+TGG 0.496224 5:-106910466 MsG0580030117.01.T01:CDS
CTAATTTCGGCCCCTATATG+TGG 0.499423 5:-106910680 MsG0580030117.01.T01:CDS
GGTCCTAAATCAAAAGGGCT+AGG 0.510204 5:+106910798 None:intergenic
TCGAATCGGAGTGGGAAAGA+AGG 0.518323 5:-106910304 MsG0580030117.01.T01:CDS
TTAGGTTCAGGAAGTGTCCA+TGG 0.532233 5:-106910900 MsG0580030117.01.T01:CDS
CGAATCGGAGTGGGAAAGAA+GGG 0.533149 5:-106910303 MsG0580030117.01.T01:CDS
ACCCAGAAGATCGAGAAACT+TGG 0.535057 5:-106910228 MsG0580030117.01.T01:CDS
TCGTTATAATTTCTTAACAA+AGG 0.537231 5:-106911108 MsG0580030117.01.T01:CDS
GATCAACAATTATTTATCGA+TGG 0.547329 5:-106910588 MsG0580030117.01.T01:CDS
TGTGTCTGTGGCTGAAGGTA+AGG 0.548749 5:+106910488 None:intergenic
CTTGATTATCGAATCGGAGT+GGG 0.568420 5:-106910312 MsG0580030117.01.T01:CDS
AATTAGGTCCTAAATCAAAA+GGG 0.568996 5:+106910793 None:intergenic
ACAGATATTATGAATCTGAA+AGG 0.576014 5:-106911028 MsG0580030117.01.T01:CDS
CCACACTGAATATGTGTCTG+TGG 0.576718 5:+106910476 None:intergenic
TCAAAACCAGAATCATACTC+TGG 0.576743 5:+106910927 None:intergenic
AAAGCAGACAAAGAATTCCA+TGG 0.579973 5:+106910883 None:intergenic
TATATATCTCCCACATATAG+GGG 0.590313 5:+106910670 None:intergenic
GACTATAAAGTGATGTGCCA+CGG 0.591228 5:-106910720 MsG0580030117.01.T01:CDS
CGATAATCAAGGCAAGGCAA+AGG 0.599455 5:+106910324 None:intergenic
TGGTTATGATTCTGTCAACA+AGG 0.600309 5:-106910742 MsG0580030117.01.T01:CDS
GAATATGTGTCTGTGGCTGA+AGG 0.615134 5:+106910483 None:intergenic
TTTCGTCAATAACTATTCCG+TGG 0.618643 5:+106910703 None:intergenic
CCTAAATCAAAAGGGCTAGG+AGG 0.626131 5:+106910801 None:intergenic
TTTGATTTAGGACCTAATTG+GGG 0.626382 5:-106910789 MsG0580030117.01.T01:CDS
CCGATTCGATAATCAAGGCA+AGG 0.632085 5:+106910318 None:intergenic
TAATTTCGGCCCCTATATGT+GGG 0.632531 5:-106910679 MsG0580030117.01.T01:CDS
GATTCATAATATCTGTCACA+CGG 0.643207 5:+106911035 None:intergenic
CCTTGATTATCGAATCGGAG+TGG 0.647462 5:-106910313 MsG0580030117.01.T01:CDS
GATCTTAAATCACACAGACA+CGG 0.649530 5:-106910418 MsG0580030117.01.T01:CDS
CCCGTAAGAATATTATATTG+TGG 0.656010 5:-106910854 MsG0580030117.01.T01:CDS
GTCATTTGACTGGAGCAATG+AGG 0.661627 5:-106910526 MsG0580030117.01.T01:CDS
AATCATAACCAAATTCACGA+TGG 0.708315 5:+106910754 None:intergenic
CCACAGACACATATTCAGTG+TGG 0.714715 5:-106910476 MsG0580030117.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTGTTTAGAAAAAAAGA+CGG - Chr5:106910989-106911008 MsG0580030117.01.T01:CDS 15.0%
!! AATAATTGTTGATCAATCAT+AGG + Chr5:106910671-106910690 None:intergenic 20.0%
!! AGAATATGGTAAAAATAGAT+TGG - Chr5:106910294-106910313 MsG0580030117.01.T01:CDS 20.0%
!! TCCACAATATAATATTCTTA+CGG + Chr5:106910415-106910434 None:intergenic 20.0%
!! TCGTTATAATTTCTTAACAA+AGG - Chr5:106910157-106910176 MsG0580030117.01.T01:CDS 20.0%
!!! ACGTTTCATATTTCAATTTT+AGG - Chr5:106910872-106910891 MsG0580030117.01.T01:CDS 20.0%
! AATTAGGTCCTAAATCAAAA+GGG + Chr5:106910475-106910494 None:intergenic 25.0%
! ACAGATATTATGAATCTGAA+AGG - Chr5:106910237-106910256 MsG0580030117.01.T01:CDS 25.0%
! CCACAATATAATATTCTTAC+GGG + Chr5:106910414-106910433 None:intergenic 25.0%
! GATCAACAATTATTTATCGA+TGG - Chr5:106910677-106910696 MsG0580030117.01.T01:CDS 25.0%
! GTTATTGACGAAACTAATTT+CGG - Chr5:106910572-106910591 MsG0580030117.01.T01:CDS 25.0%
!!! AAACAATTGGTGCTTTTAAA+TGG - Chr5:106910812-106910831 MsG0580030117.01.T01:CDS 25.0%
!!! ACGGATTCAATTTTTTATGT+AGG + Chr5:106910214-106910233 None:intergenic 25.0%
!!! GATTCAATTTTTTATGTAGG+AGG + Chr5:106910211-106910230 None:intergenic 25.0%
!!! GATTCTGGTTTTGATATTTT+AGG - Chr5:106910347-106910366 MsG0580030117.01.T01:CDS 25.0%
!!! TTTGAGTTGTATTCTATTTG+TGG - Chr5:106910260-106910279 MsG0580030117.01.T01:CDS 25.0%
!!! TTTTGATTTAGGACCTAATT+GGG - Chr5:106910475-106910494 MsG0580030117.01.T01:CDS 25.0%
AATCATAACCAAATTCACGA+TGG + Chr5:106910514-106910533 None:intergenic 30.0%
ACTATATATCTCCCACATAT+AGG + Chr5:106910600-106910619 None:intergenic 30.0%
AGCTTAAATTCCATAGTAGT+TGG + Chr5:106910440-106910459 None:intergenic 30.0%
ATAGTCTAAGAAGTAACTCT+TGG - Chr5:106910615-106910634 MsG0580030117.01.T01:CDS 30.0%
ATATTCAGTGTGGAAACAAT+TGG - Chr5:106910799-106910818 MsG0580030117.01.T01:CDS 30.0%
CAATTAGGTCCTAAATCAAA+AGG + Chr5:106910476-106910495 None:intergenic 30.0%
CCCGTAAGAATATTATATTG+TGG - Chr5:106910411-106910430 MsG0580030117.01.T01:CDS 30.0%
CTATATATCTCCCACATATA+GGG + Chr5:106910599-106910618 None:intergenic 30.0%
GATTCATAATATCTGTCACA+CGG + Chr5:106910233-106910252 None:intergenic 30.0%
TATATATCTCCCACATATAG+GGG + Chr5:106910598-106910617 None:intergenic 30.0%
TGGTGATAGATTTGAGAATA+TGG - Chr5:106910280-106910299 MsG0580030117.01.T01:CDS 30.0%
TTGTTTAGAAAAAAAGACGG+TGG - Chr5:106910992-106911011 MsG0580030117.01.T01:CDS 30.0%
! TTTGATTTAGGACCTAATTG+GGG - Chr5:106910476-106910495 MsG0580030117.01.T01:CDS 30.0%
!!! CTTTTGATTTAGGACCTAAT+TGG - Chr5:106910474-106910493 MsG0580030117.01.T01:CDS 30.0%
!!! GGTTTTGATATTTTAGGTTC+AGG - Chr5:106910353-106910372 MsG0580030117.01.T01:CDS 30.0%
AAAGCAGACAAAGAATTCCA+TGG + Chr5:106910385-106910404 None:intergenic 35.0%
AGAAAGCCTTCTTCAATTTG+AGG - Chr5:106911099-106911118 MsG0580030117.01.T01:CDS 35.0%
ATTGTGGAATCCAACTACTA+TGG - Chr5:106910427-106910446 MsG0580030117.01.T01:CDS 35.0%
GAAAGCCTTCTTCAATTTGA+GGG - Chr5:106911100-106911119 MsG0580030117.01.T01:CDS 35.0%
GATCTTAAATCACACAGACA+CGG - Chr5:106910847-106910866 MsG0580030117.01.T01:CDS 35.0%
GGCCAATCTAATAAGACATT+TGG + Chr5:106910320-106910339 None:intergenic 35.0%
TCAAAACCAGAATCATACTC+TGG + Chr5:106910341-106910360 None:intergenic 35.0%
TGGACTAAAGTCTTCATAGT+AGG - Chr5:106910917-106910936 MsG0580030117.01.T01:CDS 35.0%
TGGTTATGATTCTGTCAACA+AGG - Chr5:106910523-106910542 MsG0580030117.01.T01:CDS 35.0%
TTGATGTCGATATGCATAAC+AGG - Chr5:106910645-106910664 MsG0580030117.01.T01:CDS 35.0%
TTTCGTCAATAACTATTCCG+TGG + Chr5:106910565-106910584 None:intergenic 35.0%
! GGCCAAATGTCTTATTAGAT+TGG - Chr5:106910315-106910334 MsG0580030117.01.T01:CDS 35.0%
!! ATTTCAATTTTAGGTGCGCT+TGG - Chr5:106910881-106910900 MsG0580030117.01.T01:CDS 35.0%
!! CATATTTGCTGTCATTTGAC+TGG - Chr5:106910729-106910748 MsG0580030117.01.T01:CDS 35.0%
CCTTGCCTTGATTATCGAAT+CGG - Chr5:106910947-106910966 MsG0580030117.01.T01:CDS 40.0%
CTCTCTCATTGGATGTGTTA+TGG - Chr5:106910701-106910720 MsG0580030117.01.T01:CDS 40.0%
CTTGATTATCGAATCGGAGT+GGG - Chr5:106910953-106910972 MsG0580030117.01.T01:CDS 40.0%
GACTATAAAGTGATGTGCCA+CGG - Chr5:106910545-106910564 MsG0580030117.01.T01:CDS 40.0%
TAATTTCGGCCCCTATATGT+GGG - Chr5:106910586-106910605 MsG0580030117.01.T01:CDS 40.0%
TCAAACCAAACTCCCCAATT+AGG + Chr5:106910491-106910510 None:intergenic 40.0%
TGGTTTGACCATCGTGAATT+TGG - Chr5:106910503-106910522 MsG0580030117.01.T01:CDS 40.0%
! TTATCGATGGACTCTCTCAT+TGG - Chr5:106910690-106910709 MsG0580030117.01.T01:CDS 40.0%
!! AAAAAGACGGTGGACTAGTT+AGG - Chr5:106911002-106911021 MsG0580030117.01.T01:CDS 40.0%
ACCCAGAAGATCGAGAAACT+TGG - Chr5:106911037-106911056 MsG0580030117.01.T01:CDS 45.0%
CCACACTGAATATGTGTCTG+TGG + Chr5:106910792-106910811 None:intergenic 45.0%
CCACAGACACATATTCAGTG+TGG - Chr5:106910789-106910808 MsG0580030117.01.T01:CDS 45.0%
CCTAAATCAAAAGGGCTAGG+AGG + Chr5:106910467-106910486 None:intergenic 45.0%
CCTTGATTATCGAATCGGAG+TGG - Chr5:106910952-106910971 MsG0580030117.01.T01:CDS 45.0%
CGATAATCAAGGCAAGGCAA+AGG + Chr5:106910944-106910963 None:intergenic 45.0%
CTAATTTCGGCCCCTATATG+TGG - Chr5:106910585-106910604 MsG0580030117.01.T01:CDS 45.0%
GAATATGTGTCTGTGGCTGA+AGG + Chr5:106910785-106910804 None:intergenic 45.0%
GCCAAGTTTCTCGATCTTCT+GGG + Chr5:106911041-106911060 None:intergenic 45.0%
GGTCCTAAATCAAAAGGGCT+AGG + Chr5:106910470-106910489 None:intergenic 45.0%
GTCATTTGACTGGAGCAATG+AGG - Chr5:106910739-106910758 MsG0580030117.01.T01:CDS 45.0%
TAGGACCTAATTGGGGAGTT+TGG - Chr5:106910483-106910502 MsG0580030117.01.T01:CDS 45.0%
TGCCAAGTTTCTCGATCTTC+TGG + Chr5:106911042-106911061 None:intergenic 45.0%
TTGCGCCCTCAAATTGAAGA+AGG + Chr5:106911108-106911127 None:intergenic 45.0%
! CCACTCCGATTCGATAATCA+AGG + Chr5:106910955-106910974 None:intergenic 45.0%
! CCGATTCGATAATCAAGGCA+AGG + Chr5:106910950-106910969 None:intergenic 45.0%
! GATTGGCCAGAGTATGATTC+TGG - Chr5:106910332-106910351 MsG0580030117.01.T01:CDS 45.0%
!! CGCTTGGTGTAATGGAATCA+TGG - Chr5:106910897-106910916 MsG0580030117.01.T01:CDS 45.0%
!! TTAGGTTCAGGAAGTGTCCA+TGG - Chr5:106910365-106910384 MsG0580030117.01.T01:CDS 45.0%
!! TTTAGGTGCGCTTGGTGTAA+TGG - Chr5:106910889-106910908 MsG0580030117.01.T01:CDS 45.0%
!!! CCTCCTAGCCCTTTTGATTT+AGG - Chr5:106910464-106910483 MsG0580030117.01.T01:CDS 45.0%
CGAATCGGAGTGGGAAAGAA+GGG - Chr5:106910962-106910981 MsG0580030117.01.T01:CDS 50.0%
TCGAATCGGAGTGGGAAAGA+AGG - Chr5:106910961-106910980 MsG0580030117.01.T01:CDS 50.0%
TGTGTCTGTGGCTGAAGGTA+AGG + Chr5:106910780-106910799 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr5 gene 106910152 106911135 106910152 ID=MsG0580030117.01;Name=MsG0580030117.01
Chr5 mRNA 106910152 106911135 106910152 ID=MsG0580030117.01.T01;Parent=MsG0580030117.01;Name=MsG0580030117.01.T01;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|1|1|0|327
Chr5 exon 106910152 106911135 106910152 ID=MsG0580030117.01.T01:exon:38239;Parent=MsG0580030117.01.T01
Chr5 CDS 106910152 106911135 106910152 ID=MsG0580030117.01.T01:cds;Parent=MsG0580030117.01.T01
Gene Sequence

>MsG0580030117.01.T01

ATGTATCGTTATAATTTCTTAACAAAGGATCATTCTTATTATGATGATACGTCTCTCCTCCTACATAAAAAATTGAATCCGTGTGACAGATATTATGAATCTGAAAGGTTTGAGTTGTATTCTATTTGTGGTGATAGATTTGAGAATATGGTAAAAATAGATTGGCCAAATGTCTTATTAGATTGGCCAGAGTATGATTCTGGTTTTGATATTTTAGGTTCAGGAAGTGTCCATGGAATTCTTTGTCTGCTTTGTGCATCCCGTAAGAATATTATATTGTGGAATCCAACTACTATGGAATTTAAGCTTGTTCCTCCTAGCCCTTTTGATTTAGGACCTAATTGGGGAGTTTGGTTTGACCATCGTGAATTTGGTTATGATTCTGTCAACAAGGACTATAAAGTGATGTGCCACGGAATAGTTATTGACGAAACTAATTTCGGCCCCTATATGTGGGAGATATATAGTCTAAGAAGTAACTCTTGGAAAAAACTTGATGTCGATATGCATAACAGGCCTATGATTGATCAACAATTATTTATCGATGGACTCTCTCATTGGATGTGTTATGGTGAAACATATTTGCTGTCATTTGACTGGAGCAATGAGGTTTTCATTACAACATCCTTACCTTCAGCCACAGACACATATTCAGTGTGGAAACAATTGGTGCTTTTAAATGGATCTATTGCTTTGATCTTAAATCACACAGACACGGCTACGTTTCATATTTCAATTTTAGGTGCGCTTGGTGTAATGGAATCATGGACTAAAGTCTTCATAGTAGGACCTTTGCCTTGCCTTGATTATCGAATCGGAGTGGGAAAGAAGGGTGATATTTTGTTTAGAAAAAAAGACGGTGGACTAGTTAGGTTAAATTTAAGTACCCAGAAGATCGAGAAACTTGGCATTATAATAGAGAGATATTGTAAGATACTAATTCATAAAGAAAGCCTTCTTCAATTTGAGGGCGCAAATATTTAA
Protein sequence

>MsG0580030117.01.T01

MYRYNFLTKDHSYYDDTSLLLHKKLNPCDRYYESERFELYSICGDRFENMVKIDWPNVLLDWPEYDSGFDILGSGSVHGILCLLCASRKNIILWNPTTMEFKLVPPSPFDLGPNWGVWFDHREFGYDSVNKDYKVMCHGIVIDETNFGPYMWEIYSLRSNSWKKLDVDMHNRPMIDQQLFIDGLSHWMCYGETYLLSFDWSNEVFITTSLPSATDTYSVWKQLVLLNGSIALILNHTDTATFHISILGALGVMESWTKVFIVGPLPCLDYRIGVGKKGDILFRKKDGGLVRLNLSTQKIEKLGIIIERYCKILIHKESLLQFEGANI*