AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035294.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035294.01.T01 MTR_0129s0070 94.565 184 10 0 1 184 1 184 1.14e-124 358
MsG0680035294.01.T01 MTR_0129s0070 86.667 45 6 0 176 220 353 397 4.38e-19 85.1
MsG0680035294.01.T01 MTR_0129s0120 90.217 184 18 0 1 184 1 184 7.56e-118 341
MsG0680035294.01.T01 MTR_0129s0120 75.556 45 11 0 176 220 353 397 1.61e-14 72.0
MsG0680035294.01.T01 MTR_4g117550 72.727 187 48 1 1 184 1 187 3.56e-94 280
MsG0680035294.01.T01 MTR_0129s0050 70.000 180 48 4 7 183 4 180 3.25e-73 228
MsG0680035294.01.T01 MTR_0129s0050 59.091 44 18 0 176 219 365 408 8.41e-11 61.2
MsG0680035294.01.T01 MTR_8g063440 56.284 183 73 4 2 183 1 177 6.78e-58 187
MsG0680035294.01.T01 MTR_1g007450 54.494 178 80 1 7 183 2 179 3.40e-56 184
MsG0680035294.01.T01 MTR_8g073570 53.939 165 74 2 15 179 8 170 3.23e-55 174
MsG0680035294.01.T01 MTR_1g007410 54.301 186 80 4 2 183 7 191 4.89e-54 178
MsG0680035294.01.T01 MTR_4g014370 45.259 232 98 6 10 212 4 235 5.39e-53 176
MsG0680035294.01.T01 MTR_2g038680 52.381 189 82 4 2 182 1 189 5.87e-53 175
MsG0680035294.01.T01 MTR_5g096630 52.459 183 81 4 7 183 8 190 1.54e-52 174
MsG0680035294.01.T01 MTR_4g055820 45.413 218 106 5 5 219 2 209 1.63e-52 173
MsG0680035294.01.T01 MTR_1g053510 53.143 175 75 4 14 183 12 184 3.30e-51 171
MsG0680035294.01.T01 MTR_2g021950 49.198 187 89 5 2 183 1 186 1.08e-50 169
MsG0680035294.01.T01 MTR_2g021770 51.934 181 79 5 11 183 6 186 1.70e-50 169
MsG0680035294.01.T01 MTR_1g007430 55.063 158 70 1 28 184 7 164 2.13e-50 167
MsG0680035294.01.T01 MTR_2g022000 49.744 195 85 6 2 184 1 194 2.53e-50 169
MsG0680035294.01.T01 MTR_6g070880 50.538 186 74 7 12 183 27 208 8.43e-50 163
MsG0680035294.01.T01 MTR_6g071420 50.538 186 74 7 12 183 27 208 8.43e-50 163
MsG0680035294.01.T01 MTR_8g012090 51.795 195 68 9 2 181 1 184 1.38e-49 165
MsG0680035294.01.T01 MTR_6g086825 53.073 179 75 5 14 184 67 244 3.36e-49 167
MsG0680035294.01.T01 MTR_8g464770 51.977 177 77 5 12 183 7 180 9.80e-49 164
MsG0680035294.01.T01 MTR_5g037190 48.663 187 90 4 2 183 1 186 1.14e-48 160
MsG0680035294.01.T01 MTR_8g039500 50.000 176 76 5 11 183 6 172 1.56e-48 162
MsG0680035294.01.T01 MTR_4g102410 49.180 183 87 3 7 183 51 233 1.97e-48 164
MsG0680035294.01.T01 MTR_2g021800 46.970 198 87 6 2 184 1 195 2.27e-48 164
MsG0680035294.01.T01 MTR_8g064790 48.588 177 86 4 7 180 5 179 1.29e-47 160
MsG0680035294.01.T01 MTR_5g097220 47.594 187 79 4 10 183 6 186 2.38e-47 160
MsG0680035294.01.T01 MTR_8g046200 45.408 196 85 5 2 183 1 188 4.69e-47 160
MsG0680035294.01.T01 MTR_8g046310 48.168 191 80 7 2 183 1 181 5.22e-47 159
MsG0680035294.01.T01 MTR_2g066150 47.727 176 82 4 11 184 6 173 6.15e-47 159
MsG0680035294.01.T01 MTR_3g011120 50.000 182 82 6 7 183 4 181 3.45e-46 157
MsG0680035294.01.T01 MTR_6g465360 50.785 191 72 9 11 184 56 241 3.64e-46 154
MsG0680035294.01.T01 MTR_7g017040 52.486 181 77 6 8 183 7 183 7.68e-46 157
MsG0680035294.01.T01 MTR_5g097330 49.206 189 78 7 11 184 10 195 8.42e-46 157
MsG0680035294.01.T01 MTR_8g467670 47.541 183 90 5 6 183 2 183 1.90e-45 155
MsG0680035294.01.T01 MTR_2g104340 47.475 198 92 5 7 199 4 194 1.97e-45 155
MsG0680035294.01.T01 MTR_8g033090 50.000 174 81 4 14 183 11 182 4.16e-45 155
MsG0680035294.01.T01 MTR_0831s0010 47.977 173 80 3 14 183 47 212 7.55e-45 154
MsG0680035294.01.T01 MTR_8g033140 48.187 193 86 7 1 183 1 189 1.33e-44 154
MsG0680035294.01.T01 MTR_2g066170 46.591 176 91 3 11 184 6 180 2.45e-44 152
MsG0680035294.01.T01 MTR_5g072060 50.877 171 80 4 15 183 4 172 2.68e-44 156
MsG0680035294.01.T01 MTR_2g066200 45.810 179 90 4 11 184 6 182 3.43e-44 152
MsG0680035294.01.T01 MTR_5g037680 46.524 187 82 6 12 183 3 186 4.02e-44 153
MsG0680035294.01.T01 MTR_7g017130 47.090 189 92 5 2 183 1 188 4.03e-44 152
MsG0680035294.01.T01 MTR_7g017170 47.090 189 92 5 2 183 1 188 4.41e-44 152
MsG0680035294.01.T01 MTR_7g069550 45.652 184 93 5 7 184 4 186 5.38e-44 152
MsG0680035294.01.T01 MTR_8g013000 46.111 180 93 3 8 183 1 180 6.37e-44 152
MsG0680035294.01.T01 MTR_5g024260 49.741 193 85 8 1 184 5 194 6.97e-44 152
MsG0680035294.01.T01 MTR_2g034700 49.180 183 78 7 11 183 7 184 7.26e-44 151
MsG0680035294.01.T01 MTR_6g461660 48.677 189 80 5 8 183 10 194 1.19e-43 151
MsG0680035294.01.T01 MTR_8g046220 47.541 183 82 4 6 183 2 175 1.25e-43 151
MsG0680035294.01.T01 MTR_2g104280 46.231 199 88 6 12 197 9 201 4.29e-43 149
MsG0680035294.01.T01 MTR_6g079770 48.241 199 85 7 1 184 1 196 6.11e-43 149
MsG0680035294.01.T01 MTR_5g097820 43.137 204 101 6 1 193 7 206 1.19e-42 145
MsG0680035294.01.T01 MTR_6g080680 47.208 197 78 6 7 184 9 198 1.78e-42 148
MsG0680035294.01.T01 MTR_7g079640 46.111 180 91 5 7 183 4 180 3.31e-42 147
MsG0680035294.01.T01 MTR_6g478230 47.027 185 90 4 7 184 17 200 3.53e-42 147
MsG0680035294.01.T01 MTR_8g033110 47.159 176 83 5 14 183 11 182 4.99e-42 147
MsG0680035294.01.T01 MTR_0054s0090 43.868 212 92 8 1 192 1 205 5.55e-42 147
MsG0680035294.01.T01 MTR_6g090180 46.114 193 94 6 1 183 31 223 2.30e-41 145
MsG0680035294.01.T01 MTR_8g064890 43.684 190 95 5 2 183 1 186 3.05e-41 144
MsG0680035294.01.T01 MTR_8g032980 45.989 187 84 7 5 183 2 179 7.28e-41 144
MsG0680035294.01.T01 MTR_6g079810 46.193 197 90 6 1 184 53 246 1.51e-40 144
MsG0680035294.01.T01 MTR_0134s0060 48.677 189 79 7 11 184 6 191 2.38e-40 142
MsG0680035294.01.T01 MTR_5g096900 47.938 194 76 6 12 186 7 194 2.85e-40 140
MsG0680035294.01.T01 MTR_1g076810 49.733 187 73 8 10 183 11 189 4.51e-40 145
MsG0680035294.01.T01 MTR_7g016930 44.186 215 78 8 11 220 10 187 4.91e-40 140
MsG0680035294.01.T01 MTR_0054s0100 45.714 210 90 9 3 195 4 206 5.46e-40 141
MsG0680035294.01.T01 MTR_8g018110 46.591 176 83 6 14 183 11 181 6.82e-40 141
MsG0680035294.01.T01 MTR_8g041270 45.361 194 83 7 4 184 2 185 7.22e-40 141
MsG0680035294.01.T01 MTR_5g059430 44.554 202 81 10 7 182 16 212 9.99e-40 141
MsG0680035294.01.T01 MTR_4g028510 43.655 197 91 8 2 182 1 193 2.25e-39 141
MsG0680035294.01.T01 MTR_4g014370 43.128 211 98 8 2 190 6 216 3.39e-39 138
MsG0680035294.01.T01 MTR_2g064950 45.604 182 79 5 8 183 1 168 5.53e-39 139
MsG0680035294.01.T01 MTR_5g069390 45.078 193 87 8 7 182 16 206 1.08e-38 139
MsG0680035294.01.T01 MTR_5g074850 44.560 193 86 8 2 182 20 203 1.55e-38 141
MsG0680035294.01.T01 MTR_4g028680 42.268 194 96 5 2 182 1 191 1.94e-38 137
MsG0680035294.01.T01 MTR_5g097680 43.541 209 86 6 7 184 7 214 2.43e-38 137
MsG0680035294.01.T01 MTR_5g097350 48.649 185 78 7 12 183 11 191 3.07e-38 136
MsG0680035294.01.T01 MTR_4g028660 41.624 197 92 8 2 182 1 190 7.22e-38 135
MsG0680035294.01.T01 MTR_4g027450 41.624 197 92 8 2 182 1 190 7.22e-38 135
MsG0680035294.01.T01 MTR_5g092850 44.271 192 85 7 3 182 1 182 1.07e-37 135
MsG0680035294.01.T01 MTR_1g050308 44.068 177 90 5 11 182 28 200 1.09e-37 134
MsG0680035294.01.T01 MTR_2g065860 43.617 188 87 7 11 184 6 188 3.93e-37 134
MsG0680035294.01.T01 MTR_5g096910 40.099 202 97 5 1 184 1 196 1.93e-36 132
MsG0680035294.01.T01 MTR_5g097650 41.818 165 82 3 1 162 6 159 9.32e-36 124
MsG0680035294.01.T01 MTR_4g028750 43.158 190 91 6 7 182 5 191 1.41e-35 129
MsG0680035294.01.T01 MTR_5g075370 44.809 183 79 7 14 182 5 179 1.54e-35 133
MsG0680035294.01.T01 MTR_7g104710 47.500 160 75 5 1 155 1 156 2.29e-35 127
MsG0680035294.01.T01 MTR_5g096070 43.860 171 62 6 10 176 19 159 3.62e-35 122
MsG0680035294.01.T01 MTR_2g104260 44.712 208 94 8 7 200 4 204 4.90e-35 125
MsG0680035294.01.T01 MTR_4g130330 43.243 185 81 7 14 182 4 180 5.62e-35 128
MsG0680035294.01.T01 MTR_2g065840 40.625 192 96 8 7 184 2 189 6.23e-35 128
MsG0680035294.01.T01 MTR_8g063330 43.094 181 94 5 11 184 6 184 9.42e-34 126
MsG0680035294.01.T01 MTR_8g036970 46.259 147 73 3 21 161 2 148 2.50e-33 123
MsG0680035294.01.T01 MTR_8g063370 36.923 195 112 4 5 196 16 202 4.17e-33 123
MsG0680035294.01.T01 MTR_3g019290 38.462 182 70 4 7 183 2 146 7.65e-33 117
MsG0680035294.01.T01 MTR_7g111700 43.850 187 72 9 14 183 12 182 9.49e-33 121
MsG0680035294.01.T01 MTR_1g098180 41.176 187 86 7 1 183 1 167 1.75e-32 121
MsG0680035294.01.T01 MTR_5g091140 42.784 194 75 9 1 184 30 197 2.54e-32 121
MsG0680035294.01.T01 MTR_8g063310 39.674 184 94 7 12 190 17 188 1.03e-31 119
MsG0680035294.01.T01 MTR_6g090250 44.186 172 69 8 35 183 29 196 2.79e-27 107
MsG0680035294.01.T01 MTR_5g094350 39.153 189 79 6 13 184 9 178 4.58e-27 104
MsG0680035294.01.T01 MTR_5g022850 35.897 195 74 3 7 196 6 154 2.91e-25 101
MsG0680035294.01.T01 MTR_1g115205 41.341 179 81 4 10 188 22 176 2.38e-24 97.1
MsG0680035294.01.T01 MTR_5g092860 39.375 160 62 7 35 182 20 156 1.75e-23 97.1
MsG0680035294.01.T01 MTR_3g047580 42.069 145 54 5 11 149 7 127 1.93e-23 91.7
MsG0680035294.01.T01 MTR_5g043605 38.693 199 50 9 1 184 1 142 1.37e-21 91.7
MsG0680035294.01.T01 MTR_8g063350 34.682 173 68 4 17 184 14 146 3.03e-21 90.5
MsG0680035294.01.T01 MTR_1g047080 41.791 134 60 7 54 183 1 120 1.67e-20 87.8
MsG0680035294.01.T01 MTR_8g041440 46.364 110 31 3 12 112 10 100 3.16e-20 82.8
MsG0680035294.01.T01 MTR_4g007940 39.344 122 71 2 64 183 4 124 5.79e-20 87.0
MsG0680035294.01.T01 MTR_6g078010 39.726 146 72 7 54 186 1 143 6.39e-20 87.0
MsG0680035294.01.T01 MTR_5g049560 41.379 174 79 10 54 206 1 172 6.44e-20 85.9
MsG0680035294.01.T01 MTR_5g074800 41.085 129 74 2 56 183 1 128 1.01e-19 86.3
MsG0680035294.01.T01 MTR_6g090260 30.741 270 119 10 2 214 1 259 2.43e-19 84.0
MsG0680035294.01.T01 MTR_1g050312 43.878 98 46 3 88 183 16 106 5.83e-19 83.6
MsG0680035294.01.T01 MTR_5g086780 64.516 62 19 1 2 60 1 62 9.72e-18 79.0
MsG0680035294.01.T01 MTR_2g046900 35.882 170 89 7 19 182 30 185 1.01e-17 80.9
MsG0680035294.01.T01 MTR_5g068490 44.318 88 42 2 97 184 8 88 2.89e-15 73.2
MsG0680035294.01.T01 MTR_4g023090 30.457 197 116 7 1 192 1 181 8.15e-15 72.8
MsG0680035294.01.T01 MTR_6g027910 31.461 178 103 8 17 182 9 179 8.19e-15 72.8
MsG0680035294.01.T01 MTR_2g021150 32.530 166 101 5 19 182 16 172 8.35e-15 72.8
MsG0680035294.01.T01 MTR_7g023800 41.026 117 54 6 87 190 33 147 1.86e-14 71.2
MsG0680035294.01.T01 MTR_2g046920 31.776 214 111 12 19 219 26 217 2.15e-14 71.6
MsG0680035294.01.T01 MTR_4g023440 31.288 163 99 5 20 182 21 170 2.36e-14 71.2
MsG0680035294.01.T01 MTR_3g006670 31.902 163 102 4 20 182 52 205 2.60e-14 71.2
MsG0680035294.01.T01 MTR_1g009110 29.688 192 123 8 6 190 2 188 3.67e-14 70.9
MsG0680035294.01.T01 MTR_0044s0300 30.178 169 99 6 20 182 41 196 7.48e-14 70.1
MsG0680035294.01.T01 MTR_1g097490 33.520 179 105 7 3 180 35 200 7.83e-14 70.1
MsG0680035294.01.T01 MTR_6g087620 38.000 100 55 3 88 181 1 99 9.32e-14 68.9
MsG0680035294.01.T01 MTR_8g041490 40.741 108 31 4 12 112 10 91 1.48e-13 65.1
MsG0680035294.01.T01 MTR_4g021785 32.609 184 97 7 20 197 12 174 1.64e-13 68.9
MsG0680035294.01.T01 MTR_3g014540 32.927 164 96 6 20 182 32 182 1.75e-13 68.9
MsG0680035294.01.T01 MTR_4g080200 29.545 176 94 6 17 187 3 153 2.08e-13 68.6
MsG0680035294.01.T01 MTR_6g079790 46.154 91 42 3 100 184 2 91 2.16e-13 68.2
MsG0680035294.01.T01 MTR_4g094365 32.934 167 93 8 20 182 11 162 2.20e-13 68.6
MsG0680035294.01.T01 MTR_8g013010 29.651 172 102 6 16 182 53 210 2.55e-13 68.2
MsG0680035294.01.T01 MTR_2g078900 28.090 178 97 7 20 186 36 193 3.29e-13 68.2
MsG0680035294.01.T01 MTR_3g013740 28.261 184 105 6 12 182 39 208 3.61e-13 68.2
MsG0680035294.01.T01 MTR_3g014570 30.500 200 98 11 2 180 1 180 4.10e-13 67.8
MsG0680035294.01.T01 MTR_5g064700 35.669 157 69 7 56 184 1 153 4.43e-13 67.8
MsG0680035294.01.T01 MTR_5g022420 28.440 218 127 9 2 210 6 203 4.85e-13 67.8
MsG0680035294.01.T01 MTR_2g046830 32.948 173 89 9 19 182 27 181 1.07e-12 66.6
MsG0680035294.01.T01 MTR_4g116330 29.775 178 105 7 9 182 15 176 1.40e-12 66.2
MsG0680035294.01.T01 MTR_4g116330 29.775 178 105 7 9 182 15 176 1.66e-12 66.2
MsG0680035294.01.T01 MTR_5g091150 38.889 108 40 5 100 185 2 105 1.68e-12 64.3
MsG0680035294.01.T01 MTR_8g101580 28.814 177 95 6 25 192 54 208 1.74e-12 65.9
MsG0680035294.01.T01 MTR_4g117770 33.136 169 96 7 16 182 10 163 2.48e-12 65.5
MsG0680035294.01.T01 MTR_4g117810 33.136 169 96 7 16 182 10 163 2.48e-12 65.5
MsG0680035294.01.T01 MTR_4g023080 32.418 182 100 8 20 197 12 174 2.50e-12 65.5
MsG0680035294.01.T01 MTR_5g098950 49.153 59 30 0 135 193 88 146 3.28e-12 64.7
MsG0680035294.01.T01 MTR_2g021860 40.659 91 48 4 98 184 13 101 3.76e-12 62.0
MsG0680035294.01.T01 MTR_2g047832 32.370 173 90 9 19 182 31 185 5.54e-12 64.7
MsG0680035294.01.T01 MTR_3g006570 29.379 177 100 8 20 188 24 183 6.00e-12 64.3
MsG0680035294.01.T01 MTR_3g013770 30.909 165 99 5 20 180 36 189 6.95e-12 64.3
MsG0680035294.01.T01 MTR_4g117780 34.969 163 93 6 20 182 14 163 8.39e-12 63.9
MsG0680035294.01.T01 MTR_3g058470 31.638 177 94 9 20 182 40 203 8.88e-12 63.9
MsG0680035294.01.T01 MTR_1g068835 31.288 163 88 8 25 182 12 155 1.27e-11 63.5
MsG0680035294.01.T01 MTR_6g048140 30.457 197 102 10 20 195 42 224 1.32e-11 63.5
MsG0680035294.01.T01 MTR_2g024110 27.879 165 102 4 20 183 7 155 1.72e-11 63.2
MsG0680035294.01.T01 MTR_3g034130 29.670 182 111 6 12 190 4 171 1.72e-11 63.2
MsG0680035294.01.T01 MTR_0115s0020 34.483 174 91 11 20 182 13 174 1.75e-11 63.2
MsG0680035294.01.T01 MTR_5g070130 29.231 195 115 8 11 200 5 181 2.29e-11 62.8
MsG0680035294.01.T01 MTR_3g011100 29.167 168 103 5 20 182 238 394 2.57e-11 62.8
MsG0680035294.01.T01 MTR_8g073550 31.638 177 84 9 20 182 33 186 2.84e-11 61.6
MsG0680035294.01.T01 MTR_2g055970 29.310 174 106 6 11 182 205 363 2.98e-11 62.8
MsG0680035294.01.T01 MTR_6g087660 43.750 80 41 2 1 76 1 80 4.74e-11 60.1
MsG0680035294.01.T01 MTR_1g068905 27.545 167 97 7 20 181 7 154 5.36e-11 61.2
MsG0680035294.01.T01 MTR_2g078850 26.966 178 99 8 20 186 33 190 5.74e-11 61.6
MsG0680035294.01.T01 MTR_4g133170 26.220 164 97 6 25 182 12 157 6.85e-11 61.2
MsG0680035294.01.T01 MTR_3g009310 30.286 175 92 9 20 182 45 201 7.90e-11 61.2
MsG0680035294.01.T01 MTR_5g011200 27.273 198 121 8 1 188 1 185 8.88e-11 60.8
MsG0680035294.01.T01 MTR_3g034160 28.729 181 116 6 11 190 3 171 8.97e-11 60.8
MsG0680035294.01.T01 MTR_5g076490 34.783 138 60 4 54 182 1 117 9.72e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035294.01.T01 AT3G16210 28.977 176 94 7 13 184 1 149 9.84e-14 69.7

Find 54 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGAAGAGCTTGGTCTTAA+AGG 0.197117 6:+101602379 MsG0680035294.01.T01:CDS
CGGTACATGTTCATGAAATT+AGG 0.201897 6:-101601959 None:intergenic
CTTGGTCTTAAAGGGGAATT+TGG 0.203493 6:+101602388 MsG0680035294.01.T01:CDS
GAGAACCTGTTGTTATATTA+TGG 0.240459 6:+101602203 MsG0680035294.01.T01:CDS
CAAAGATTTAAGAGGCAATT+TGG 0.243362 6:-101601888 None:intergenic
TATTATTATGTTCATGGATT+TGG 0.246010 6:+101602298 MsG0680035294.01.T01:CDS
TGGTGAGAGGTTTGAGAATA+AGG 0.252574 6:+101602084 MsG0680035294.01.T01:CDS
AAATATAACACATGAAGATT+TGG 0.282976 6:-101602055 None:intergenic
ACATGAAGATTTGGGAAATA+TGG 0.322530 6:-101602046 None:intergenic
GAAGTCTATTATTATGTTCA+TGG 0.330358 6:+101602292 MsG0680035294.01.T01:CDS
TTGAAGAGCTTGGTCTTAAA+GGG 0.333229 6:+101602380 MsG0680035294.01.T01:CDS
CAATCATTTATTTGTCCAAT+AGG 0.335987 6:-101602448 None:intergenic
ACCTTGTATTCCTCGGTAGC+TGG 0.336170 6:-101602229 None:intergenic
AAGGAAAGCTCTCTTCCTAT+TGG 0.360100 6:+101602433 MsG0680035294.01.T01:CDS
AACCGTTTCAAAGATTTAAG+AGG 0.380298 6:-101601896 None:intergenic
AGAATAAGGTCAAATTAGAT+TGG 0.382141 6:+101602098 MsG0680035294.01.T01:CDS
AATATAACACATGAAGATTT+GGG 0.388508 6:-101602054 None:intergenic
TACAGGTGACTCGGTAGGGC+TGG 0.404674 6:-101602260 None:intergenic
TTTCATATCCAGTACTTATG+AGG 0.414154 6:+101601988 MsG0680035294.01.T01:CDS
AAGAGGCAATTTGGACAGAA+TGG 0.425116 6:-101601879 None:intergenic
GACCAAGCTCTTCAATCACT+TGG 0.429184 6:-101602372 None:intergenic
CAAACAATGCCGTTAATAAC+CGG 0.442912 6:-101602166 None:intergenic
ACAGGTGACTCGGTAGGGCT+GGG 0.450049 6:-101602259 None:intergenic
CGAGTCACCTGTACTTTATG+AGG 0.451084 6:+101602270 MsG0680035294.01.T01:CDS
AGATTCTGTTAGAGCTGAAA+AGG 0.451160 6:+101601823 MsG0680035294.01.T01:CDS
GCTGTGTTATACTCTTGAAA+TGG 0.454283 6:-101602127 None:intergenic
GGCATTGTTTGCCTCTATCA+AGG 0.454494 6:+101602178 MsG0680035294.01.T01:CDS
ACTCGGTAGGGCTGGGAGGA+AGG 0.462800 6:-101602252 None:intergenic
TGCCTCTTAAATCTTTGAAA+CGG 0.468477 6:+101601894 MsG0680035294.01.T01:CDS
TACTGGATATGAAATAATTA+CGG 0.474142 6:-101601979 None:intergenic
GGGTTCCATAATATAACAAC+AGG 0.491869 6:-101602208 None:intergenic
CCTTGTATTCCTCGGTAGCT+GGG 0.492565 6:-101602228 None:intergenic
TTGTCAGATTGTCAATTACA+AGG 0.494312 6:+101602414 MsG0680035294.01.T01:CDS
ACCATCCTCCTCATAAGTAC+TGG 0.495470 6:-101601996 None:intergenic
CCCAGCTACCGAGGAATACA+AGG 0.507258 6:+101602228 MsG0680035294.01.T01:CDS
AATATGGCAGAGTCTGTTGT+AGG 0.526099 6:-101602030 None:intergenic
CTCATAAAGTACAGGTGACT+CGG 0.527477 6:-101602269 None:intergenic
AAAGTACAGGTGACTCGGTA+GGG 0.549427 6:-101602264 None:intergenic
TAAAGTACAGGTGACTCGGT+AGG 0.557713 6:-101602265 None:intergenic
CAGGTTCTCGTCCTTGATAG+AGG 0.563392 6:-101602189 None:intergenic
ACCCAAGTGATTGAAGAGCT+TGG 0.565240 6:+101602370 MsG0680035294.01.T01:CDS
TTAGCTGCGTACGTAAATCA+TGG 0.567892 6:+101601918 MsG0680035294.01.T01:CDS
TAGACTTCCTCATAAAGTAC+AGG 0.569050 6:-101602277 None:intergenic
CTTTGTAGTCATCTCTAACA+TGG 0.576554 6:-101602327 None:intergenic
AGGAAGGACCTTGTATTCCT+CGG 0.585881 6:-101602236 None:intergenic
TGAAGAGCTTGGTCTTAAAG+GGG 0.598562 6:+101602381 MsG0680035294.01.T01:CDS
ATGGCAGAGTCTGTTGTAGG+AGG 0.603888 6:-101602027 None:intergenic
ACCAAGCTCTTCAATCACTT+GGG 0.604380 6:-101602371 None:intergenic
CATATCCAGTACTTATGAGG+AGG 0.606225 6:+101601991 MsG0680035294.01.T01:CDS
TCCAGTACTTATGAGGAGGA+TGG 0.622047 6:+101601995 MsG0680035294.01.T01:CDS
GTGTTATACTCTTGAAATGG+AGG 0.637145 6:-101602124 None:intergenic
ATTATGGAACCCAGCTACCG+AGG 0.639079 6:+101602219 MsG0680035294.01.T01:CDS
GGTGACTCGGTAGGGCTGGG+AGG 0.644676 6:-101602256 None:intergenic
TTATACTCTTGAAATGGAGG+TGG 0.650276 6:-101602121 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATAACACATGAAGATT+TGG - Chr6:101602058-101602077 None:intergenic 20.0%
!! AATATAACACATGAAGATTT+GGG - Chr6:101602057-101602076 None:intergenic 20.0%
!! TACTGGATATGAAATAATTA+CGG - Chr6:101601982-101602001 None:intergenic 20.0%
!! TATTATTATGTTCATGGATT+TGG + Chr6:101602298-101602317 MsG0680035294.01.T01:CDS 20.0%
! AGAATAAGGTCAAATTAGAT+TGG + Chr6:101602098-101602117 MsG0680035294.01.T01:CDS 25.0%
! CAATCATTTATTTGTCCAAT+AGG - Chr6:101602451-101602470 None:intergenic 25.0%
! GAAGTCTATTATTATGTTCA+TGG + Chr6:101602292-101602311 MsG0680035294.01.T01:CDS 25.0%
!! AACACAGCTATTCATATTTT+AGG + Chr6:101602142-101602161 MsG0680035294.01.T01:CDS 25.0%
!!! CATGTGTTATATTTGCTTTT+TGG + Chr6:101602064-101602083 MsG0680035294.01.T01:CDS 25.0%
AACCGTTTCAAAGATTTAAG+AGG - Chr6:101601899-101601918 None:intergenic 30.0%
ACATGAAGATTTGGGAAATA+TGG - Chr6:101602049-101602068 None:intergenic 30.0%
CAAAGATTTAAGAGGCAATT+TGG - Chr6:101601891-101601910 None:intergenic 30.0%
GAGAACCTGTTGTTATATTA+TGG + Chr6:101602203-101602222 MsG0680035294.01.T01:CDS 30.0%
TTGTCAGATTGTCAATTACA+AGG + Chr6:101602414-101602433 MsG0680035294.01.T01:CDS 30.0%
TTTCATATCCAGTACTTATG+AGG + Chr6:101601988-101602007 MsG0680035294.01.T01:CDS 30.0%
! TGCCTCTTAAATCTTTGAAA+CGG + Chr6:101601894-101601913 MsG0680035294.01.T01:CDS 30.0%
!! AGCTATTCATATTTTAGGTC+CGG + Chr6:101602147-101602166 MsG0680035294.01.T01:CDS 30.0%
!! ATTTTAGGTCCGGTTATTAA+CGG + Chr6:101602157-101602176 MsG0680035294.01.T01:CDS 30.0%
!!! TATATTTGCTTTTTGGTGAG+AGG + Chr6:101602071-101602090 MsG0680035294.01.T01:CDS 30.0%
AGATTCTGTTAGAGCTGAAA+AGG + Chr6:101601823-101601842 MsG0680035294.01.T01:CDS 35.0%
CAAACAATGCCGTTAATAAC+CGG - Chr6:101602169-101602188 None:intergenic 35.0%
CGGTACATGTTCATGAAATT+AGG - Chr6:101601962-101601981 None:intergenic 35.0%
CTTTGTAGTCATCTCTAACA+TGG - Chr6:101602330-101602349 None:intergenic 35.0%
GCTGTGTTATACTCTTGAAA+TGG - Chr6:101602130-101602149 None:intergenic 35.0%
GGGTTCCATAATATAACAAC+AGG - Chr6:101602211-101602230 None:intergenic 35.0%
GTGTTATACTCTTGAAATGG+AGG - Chr6:101602127-101602146 None:intergenic 35.0%
TAGACTTCCTCATAAAGTAC+AGG - Chr6:101602280-101602299 None:intergenic 35.0%
TTATACTCTTGAAATGGAGG+TGG - Chr6:101602124-101602143 None:intergenic 35.0%
!! ATTGAAGAGCTTGGTCTTAA+AGG + Chr6:101602379-101602398 MsG0680035294.01.T01:CDS 35.0%
!! TTGAAGAGCTTGGTCTTAAA+GGG + Chr6:101602380-101602399 MsG0680035294.01.T01:CDS 35.0%
AAGAGGCAATTTGGACAGAA+TGG - Chr6:101601882-101601901 None:intergenic 40.0%
AAGGAAAGCTCTCTTCCTAT+TGG + Chr6:101602433-101602452 MsG0680035294.01.T01:CDS 40.0%
AATATGGCAGAGTCTGTTGT+AGG - Chr6:101602033-101602052 None:intergenic 40.0%
ACCAAGCTCTTCAATCACTT+GGG - Chr6:101602374-101602393 None:intergenic 40.0%
CATATCCAGTACTTATGAGG+AGG + Chr6:101601991-101602010 MsG0680035294.01.T01:CDS 40.0%
CTCATAAAGTACAGGTGACT+CGG - Chr6:101602272-101602291 None:intergenic 40.0%
TGGTGAGAGGTTTGAGAATA+AGG + Chr6:101602084-101602103 MsG0680035294.01.T01:CDS 40.0%
TTAGCTGCGTACGTAAATCA+TGG + Chr6:101601918-101601937 MsG0680035294.01.T01:CDS 40.0%
!! CTTGGTCTTAAAGGGGAATT+TGG + Chr6:101602388-101602407 MsG0680035294.01.T01:CDS 40.0%
!! TGAAGAGCTTGGTCTTAAAG+GGG + Chr6:101602381-101602400 MsG0680035294.01.T01:CDS 40.0%
ACCATCCTCCTCATAAGTAC+TGG - Chr6:101601999-101602018 None:intergenic 45.0%
ACCCAAGTGATTGAAGAGCT+TGG + Chr6:101602370-101602389 MsG0680035294.01.T01:CDS 45.0%
AGGAAGGACCTTGTATTCCT+CGG - Chr6:101602239-101602258 None:intergenic 45.0%
CGAGTCACCTGTACTTTATG+AGG + Chr6:101602270-101602289 MsG0680035294.01.T01:CDS 45.0%
GACCAAGCTCTTCAATCACT+TGG - Chr6:101602375-101602394 None:intergenic 45.0%
GGCATTGTTTGCCTCTATCA+AGG + Chr6:101602178-101602197 MsG0680035294.01.T01:CDS 45.0%
TCCAGTACTTATGAGGAGGA+TGG + Chr6:101601995-101602014 MsG0680035294.01.T01:CDS 45.0%
!! AAAGTACAGGTGACTCGGTA+GGG - Chr6:101602267-101602286 None:intergenic 45.0%
!! TAAAGTACAGGTGACTCGGT+AGG - Chr6:101602268-101602287 None:intergenic 45.0%
ACCTTGTATTCCTCGGTAGC+TGG - Chr6:101602232-101602251 None:intergenic 50.0%
ATGGCAGAGTCTGTTGTAGG+AGG - Chr6:101602030-101602049 None:intergenic 50.0%
ATTATGGAACCCAGCTACCG+AGG + Chr6:101602219-101602238 MsG0680035294.01.T01:CDS 50.0%
CAGGTTCTCGTCCTTGATAG+AGG - Chr6:101602192-101602211 None:intergenic 50.0%
CCTTGTATTCCTCGGTAGCT+GGG - Chr6:101602231-101602250 None:intergenic 50.0%
CCCAGCTACCGAGGAATACA+AGG + Chr6:101602228-101602247 MsG0680035294.01.T01:CDS 55.0%
!! ACAGGTGACTCGGTAGGGCT+GGG - Chr6:101602262-101602281 None:intergenic 60.0%
!! TACAGGTGACTCGGTAGGGC+TGG - Chr6:101602263-101602282 None:intergenic 60.0%
ACTCGGTAGGGCTGGGAGGA+AGG - Chr6:101602255-101602274 None:intergenic 65.0%
!! GGTGACTCGGTAGGGCTGGG+AGG - Chr6:101602259-101602278 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr6 gene 101601809 101602471 101601809 ID=MsG0680035294.01;Name=MsG0680035294.01
Chr6 mRNA 101601809 101602471 101601809 ID=MsG0680035294.01.T01;Parent=MsG0680035294.01;Name=MsG0680035294.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|220
Chr6 exon 101601809 101602471 101601809 ID=MsG0680035294.01.T01:exon:10753;Parent=MsG0680035294.01.T01
Chr6 CDS 101601809 101602471 101601809 ID=MsG0680035294.01.T01:cds;Parent=MsG0680035294.01.T01
Gene Sequence

>MsG0680035294.01.T01

ATGATGCAATCAACAGATTCTGTTAGAGCTGAAAAGGTTAGCAATCATATTCATGATGATCTTGCATTTTCCATTCTGTCCAAATTGCCTCTTAAATCTTTGAAACGGTTTAGCTGCGTACGTAAATCATGGTCTTGCTTATTTGAGAATCCTAATTTCATGAACATGTACCGTAATTATTTCATATCCAGTACTTATGAGGAGGATGGTTCACGTCTCCTCCTACAACAGACTCTGCCATATTTCCCAAATCTTCATGTGTTATATTTGCTTTTTGGTGAGAGGTTTGAGAATAAGGTCAAATTAGATTGGCCACCTCCATTTCAAGAGTATAACACAGCTATTCATATTTTAGGTCCGGTTATTAACGGCATTGTTTGCCTCTATCAAGGACGAGAACCTGTTGTTATATTATGGAACCCAGCTACCGAGGAATACAAGGTCCTTCCTCCCAGCCCTACCGAGTCACCTGTACTTTATGAGGAAGTCTATTATTATGTTCATGGATTTGGTTATGACCATGTTAGAGATGACTACAAAGTCATTCGATTTAATTTAAGTACCCAAGTGATTGAAGAGCTTGGTCTTAAAGGGGAATTTGGTTGTTGTCAGATTGTCAATTACAAGGAAAGCTCTCTTCCTATTGGACAAATAAATGATTGA
Protein sequence

>MsG0680035294.01.T01

MMQSTDSVRAEKVSNHIHDDLAFSILSKLPLKSLKRFSCVRKSWSCLFENPNFMNMYRNYFISSTYEEDGSRLLLQQTLPYFPNLHVLYLLFGERFENKVKLDWPPPFQEYNTAIHILGPVINGIVCLYQGREPVVILWNPATEEYKVLPPSPTESPVLYEEVYYYVHGFGYDHVRDDYKVIRFNLSTQVIEELGLKGEFGCCQIVNYKESSLPIGQIND*