AlfalfaGEDB | MsG0580025458.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025458.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025458.01.T01	XP_013443615.1	92.063	189	15	0	1	189	154	342	8.79E-123	363

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025458.01.T01	Q9M2A1	64.583	192	68	0	1	192	171	362	1.16E-85	261

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025458.01.T01	A0A072TVU0	92.063	189	15	0	1	189	154	342	4.20e-123	363

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048332.01	MsG0580025458.01	0.811833	5.745691e-51	3.593852e-48
MsG0080048787.01	MsG0580025458.01	0.834623	2.693486e-56	3.214080e-53
MsG0180000114.01	MsG0580025458.01	0.830792	2.406000e-55	2.561448e-52
MsG0180000144.01	MsG0580025458.01	0.809033	2.310968e-50	1.342200e-47
MsG0180000703.01	MsG0580025458.01	0.805606	1.230750e-49	6.539382e-47
MsG0180000777.01	MsG0580025458.01	0.805127	1.550956e-49	8.140277e-47
MsG0180000787.01	MsG0580025458.01	0.823254	1.529378e-53	1.309959e-50
MsG0180000836.01	MsG0580025458.01	0.803469	3.433990e-49	1.727055e-46
MsG0180000914.01	MsG0580025458.01	0.806640	7.455486e-50	4.067368e-47
MsG0180001311.01	MsG0580025458.01	0.811502	6.779511e-51	4.203899e-48
MsG0180001662.01	MsG0580025458.01	0.812467	4.178219e-51	2.658837e-48
MsG0180002399.01	MsG0580025458.01	0.819753	9.840522e-53	7.639606e-50
MsG0180002664.01	MsG0580025458.01	0.807685	4.479150e-50	2.510868e-47
MsG0180002867.01	MsG0580025458.01	0.805451	1.326228e-49	7.018952e-47
MsG0180003725.01	MsG0580025458.01	0.833894	4.102742e-56	4.790276e-53
MsG0180003807.01	MsG0580025458.01	0.829618	4.652944e-55	4.787322e-52
MsG0180003810.01	MsG0580025458.01	0.813503	2.477112e-51	1.620569e-48
MsG0180003821.01	MsG0580025458.01	0.805850	1.093908e-49	5.849309e-47
MsG0180003885.01	MsG0580025458.01	0.825324	4.988177e-54	4.532156e-51
MsG0180003922.01	MsG0580025458.01	0.800402	1.465870e-48	6.819329e-46
MsG0180003932.01	MsG0580025458.01	0.826928	2.072564e-54	1.971368e-51
MsG0180004249.01	MsG0580025458.01	0.801015	1.099224e-48	5.194053e-46
MsG0180004331.01	MsG0580025458.01	0.804146	2.484883e-49	1.271646e-46
MsG0180004465.01	MsG0580025458.01	0.808882	2.489429e-50	1.439995e-47
MsG0180004486.01	MsG0580025458.01	0.822276	2.583223e-53	2.152219e-50
MsG0180004493.01	MsG0580025458.01	0.819602	1.065264e-52	8.234996e-50
MsG0180004500.01	MsG0580025458.01	0.803668	3.123746e-49	1.579003e-46
MsG0180004765.01	MsG0580025458.01	0.805114	1.560670e-49	8.188286e-47
MsG0180005205.01	MsG0580025458.01	0.827557	1.464751e-54	1.418885e-51
MsG0180005240.01	MsG0580025458.01	0.805087	1.581236e-49	8.290511e-47
MsG0180005445.01	MsG0580025458.01	0.811057	8.468308e-51	5.189721e-48
MsG0180005730.01	MsG0580025458.01	0.801049	1.081598e-48	5.115196e-46
MsG0180005786.01	MsG0580025458.01	0.809376	1.951374e-50	1.143845e-47
MsG0180005787.01	MsG0580025458.01	0.814070	1.857505e-51	1.234026e-48
MsG0180006017.01	MsG0580025458.01	0.817049	4.034602e-52	2.907133e-49
MsG0180006096.01	MsG0580025458.01	0.825788	3.873229e-54	3.565899e-51
MsG0180006104.01	MsG0580025458.01	0.800853	1.186149e-48	5.581668e-46
MsG0180006225.01	MsG0580025458.01	0.804541	2.055584e-49	1.062666e-46
MsG0280006309.01	MsG0580025458.01	0.809579	1.764895e-50	1.040161e-47
MsG0280010599.01	MsG0580025458.01	0.846202	2.518409e-59	4.301436e-56
MsG0280010722.01	MsG0580025458.01	0.827674	1.373333e-54	1.334880e-51
MsG0280010753.01	MsG0580025458.01	0.820497	6.649990e-53	5.270261e-50
MsG0280010916.01	MsG0580025458.01	0.811832	5.747919e-51	3.595170e-48
MsG0280010976.01	MsG0580025458.01	0.802641	5.093707e-49	2.508052e-46
MsG0280011109.01	MsG0580025458.01	0.807344	5.292910e-50	2.940848e-47
MsG0280011201.01	MsG0580025458.01	0.816923	4.306121e-52	3.092030e-49
MsG0280011444.01	MsG0580025458.01	0.811256	7.668278e-51	4.723925e-48
MsG0380011537.01	MsG0580025458.01	0.808777	2.621661e-50	1.512260e-47
MsG0380011581.01	MsG0580025458.01	0.804466	2.131594e-49	1.099815e-46
MsG0380011669.01	MsG0580025458.01	0.806082	9.776889e-50	5.258688e-47
MsG0380012067.01	MsG0580025458.01	0.821410	4.098413e-53	3.331648e-50
MsG0380012371.01	MsG0580025458.01	0.811313	7.452359e-51	4.597615e-48
MsG0380013085.01	MsG0580025458.01	0.802232	6.186270e-49	3.014453e-46
MsG0380014706.01	MsG0580025458.01	0.857785	1.288546e-62	3.217095e-59
MsG0480019035.01	MsG0580025458.01	0.801574	8.446119e-49	4.047345e-46
MsG0480019453.01	MsG0580025458.01	0.853145	2.899089e-61	6.202105e-58
MsG0480019711.01	MsG0580025458.01	0.802820	4.679348e-49	2.314725e-46
MsG0480019970.01	MsG0580025458.01	0.801650	8.145580e-49	3.910824e-46
MsG0480020024.01	MsG0580025458.01	0.847530	1.090870e-59	1.944899e-56
MsG0480020505.01	MsG0580025458.01	0.831145	1.970340e-55	2.119626e-52
MsG0480020788.01	MsG0580025458.01	0.812307	4.528945e-51	2.869459e-48
MsG0480020887.01	MsG0580025458.01	0.801085	1.063562e-48	5.034400e-46
MsG0480021195.01	MsG0580025458.01	0.821770	3.382831e-53	2.778092e-50
MsG0480021492.01	MsG0580025458.01	0.814925	1.201594e-51	8.168839e-49
MsG0480021534.01	MsG0580025458.01	0.827549	1.471469e-54	1.425097e-51
MsG0480021796.01	MsG0580025458.01	0.816570	5.165788e-52	3.673643e-49
MsG0480021918.01	MsG0580025458.01	0.818002	2.460446e-52	1.819743e-49
MsG0480022169.01	MsG0580025458.01	0.808577	2.892395e-50	1.659798e-47
MsG0480022514.01	MsG0580025458.01	0.814560	1.447728e-51	9.746470e-49
MsG0480022624.01	MsG0580025458.01	0.812626	3.857569e-51	2.464839e-48
MsG0480022672.01	MsG0580025458.01	0.800081	1.703871e-48	7.862107e-46
MsG0480022827.01	MsG0580025458.01	0.802156	6.411714e-49	3.118342e-46
MsG0480023172.01	MsG0580025458.01	0.835917	1.269144e-56	1.574399e-53
MsG0480023354.01	MsG0580025458.01	0.825081	5.694665e-54	5.138361e-51
MsG0480023384.01	MsG0580025458.01	0.812889	3.378498e-51	2.173745e-48
MsG0480023488.01	MsG0580025458.01	0.818906	1.535212e-52	1.164201e-49
MsG0580024304.01	MsG0580025458.01	0.821888	3.177165e-53	2.617953e-50
MsG0580024790.01	MsG0580025458.01	-0.805008	1.642047e-49	8.591866e-47
MsG0580025332.01	MsG0580025458.01	0.801065	1.073548e-48	5.079055e-46
MsG0580025458.01	MsG0580025460.01	0.968340	1.542161e-128	1.275430e-122
MsG0580025458.01	MsG0580025661.01	0.833986	3.890093e-56	4.554671e-53
MsG0580025458.01	MsG0580025922.01	0.805229	1.476779e-49	7.770846e-47
MsG0580025458.01	MsG0580025987.01	0.821072	4.903850e-53	3.949522e-50
MsG0580025458.01	MsG0580026086.01	0.800189	1.619779e-48	7.494481e-46
MsG0580025458.01	MsG0580026137.01	0.803457	3.453987e-49	1.736572e-46
MsG0580025458.01	MsG0580026439.01	0.806493	8.009251e-50	4.353284e-47
MsG0580025458.01	MsG0580028175.01	0.958384	2.692748e-116	9.954345e-111
MsG0580025458.01	MsG0580028582.01	0.822081	2.867315e-53	2.375464e-50
MsG0580025458.01	MsG0580028754.01	0.805391	1.365744e-49	7.216630e-47
MsG0580025458.01	MsG0580028918.01	0.808997	2.351947e-50	1.364693e-47
MsG0580025458.01	MsG0580029236.01	0.825109	5.607053e-54	5.063158e-51
MsG0580025458.01	MsG0580029376.01	0.829956	3.850536e-55	4.000850e-52
MsG0580025458.01	MsG0580029550.01	0.814408	1.564249e-51	1.048813e-48
MsG0580025458.01	MsG0580029647.01	0.844073	9.476105e-59	1.512486e-55
MsG0580025458.01	MsG0580029650.01	0.803082	4.131669e-49	2.057242e-46
MsG0580025458.01	MsG0580029651.01	0.816009	6.897193e-52	4.830259e-49
MsG0580025458.01	MsG0580029879.01	0.828150	1.055145e-54	1.040041e-51
MsG0580025458.01	MsG0580029919.01	0.856229	3.704164e-62	8.779328e-59
MsG0580025458.01	MsG0580029921.01	0.808829	2.555101e-50	1.475935e-47
MsG0580025458.01	MsG0580029925.01	0.834602	2.726352e-56	3.251357e-53
MsG0580025458.01	MsG0580030142.01	0.819338	1.223893e-52	9.390990e-50
MsG0580025458.01	MsG0680030515.01	0.817746	2.810314e-52	2.064047e-49
MsG0580025458.01	MsG0680030616.01	0.805186	1.507194e-49	7.922444e-47
MsG0580025458.01	MsG0680030880.01	0.817095	3.938407e-52	2.841292e-49
MsG0580025458.01	MsG0680030881.01	0.812764	3.597782e-51	2.307186e-48
MsG0580025458.01	MsG0680031000.01	0.814355	1.607080e-51	1.075913e-48
MsG0580025458.01	MsG0680031815.01	0.813295	2.752057e-51	1.790190e-48
MsG0580025458.01	MsG0680032832.01	0.800540	1.373758e-48	6.413126e-46
MsG0580025458.01	MsG0680034235.01	0.849998	2.255299e-60	4.354522e-57
MsG0580025458.01	MsG0680034913.01	0.815631	8.374619e-52	5.804937e-49
MsG0580025458.01	MsG0680035216.01	0.842500	2.490058e-58	3.782816e-55
MsG0580025458.01	MsG0780035937.01	0.843381	1.451501e-58	2.267118e-55
MsG0580025458.01	MsG0780036117.01	0.806296	8.810951e-50	4.765123e-47
MsG0580025458.01	MsG0780036183.01	0.816251	6.090263e-52	4.293553e-49
MsG0580025458.01	MsG0780036664.01	0.801206	1.004238e-48	4.767933e-46
MsG0580025458.01	MsG0780036943.01	0.808382	3.184042e-50	1.817938e-47
MsG0580025458.01	MsG0780037330.01	0.820621	6.228075e-53	4.952489e-50
MsG0580025458.01	MsG0780037399.01	0.816347	5.794854e-52	4.096142e-49
MsG0580025458.01	MsG0780039155.01	0.835511	1.608526e-56	1.971230e-53
MsG0580025458.01	MsG0780039170.01	0.823111	1.651372e-53	1.408894e-50
MsG0580025458.01	MsG0780039178.01	0.805272	1.446398e-49	7.619398e-47
MsG0580025458.01	MsG0780039369.01	0.826372	2.812562e-54	2.632839e-51
MsG0580025458.01	MsG0780039439.01	0.807293	5.424516e-50	3.010086e-47
MsG0580025458.01	MsG0780039443.01	0.833119	6.404526e-56	7.306341e-53
MsG0580025458.01	MsG0780039717.01	0.812812	3.512414e-51	2.255139e-48
MsG0580025458.01	MsG0780040888.01	0.811529	6.690507e-51	4.151537e-48
MsG0580025458.01	MsG0780041127.01	0.828116	1.075098e-54	1.058696e-51
MsG0580025458.01	MsG0780041229.01	0.812718	3.682178e-51	2.358536e-48
MsG0580025458.01	MsG0780041387.01	0.826419	2.741354e-54	2.569567e-51
MsG0580025458.01	MsG0780041435.01	0.816258	6.068333e-52	4.278953e-49
MsG0580025458.01	MsG0780041482.01	0.839163	1.867366e-57	2.556990e-54
MsG0580025458.01	MsG0780041634.01	0.818221	2.195124e-52	1.633243e-49
MsG0580025458.01	MsG0780041729.01	-0.813425	2.576500e-51	1.681985e-48
MsG0580025458.01	MsG0880041854.01	0.816917	4.319609e-52	3.101155e-49
MsG0580025458.01	MsG0880042520.01	0.811294	7.522507e-51	4.638576e-48
MsG0580025458.01	MsG0880042857.01	0.809257	2.068954e-50	1.208910e-47
MsG0580025458.01	MsG0880043157.01	0.811961	5.387028e-51	3.381151e-48
MsG0580025458.01	MsG0880043480.01	0.811737	6.028607e-51	3.761358e-48
MsG0580025458.01	MsG0880043521.01	0.812787	3.555543e-51	2.281431e-48
MsG0580025458.01	MsG0880044131.01	0.801224	9.958277e-49	4.730164e-46
MsG0580025458.01	MsG0880044573.01	0.811494	6.807687e-51	4.220400e-48
MsG0580025458.01	MsG0880044948.01	0.834626	2.688576e-56	3.208558e-53
MsG0580025458.01	MsG0880044950.01	0.817242	3.650428e-52	2.644130e-49
MsG0580025458.01	MsG0880045111.01	0.804812	1.804645e-49	9.395786e-47
MsG0580025458.01	MsG0880045460.01	0.832711	8.089677e-56	9.116019e-53
MsG0580025458.01	MsG0880045613.01	0.806053	9.915323e-50	5.329265e-47
MsG0580025458.01	MsG0880045765.01	0.819188	1.323915e-52	1.011724e-49
MsG0580025458.01	MsG0880046517.01	0.809089	2.247950e-50	1.307610e-47
MsG0580025458.01	MsG0880046901.01	0.814930	1.198939e-51	8.151832e-49
MsG0580025458.01	MsG0880047696.01	0.858957	5.764501e-63	1.497454e-59
MsG0380014948.01	MsG0580025458.01	0.824527	7.691336e-54	6.830313e-51
MsG0380015220.01	MsG0580025458.01	0.812539	4.030285e-51	2.569440e-48
MsG0380015436.01	MsG0580025458.01	0.815048	1.128603e-51	7.698383e-49
MsG0380015930.01	MsG0580025458.01	0.823989	1.029180e-53	9.000306e-51
MsG0380016387.01	MsG0580025458.01	0.842334	2.755950e-58	4.165063e-55
MsG0380016391.01	MsG0580025458.01	0.814430	1.547297e-51	1.038006e-48
MsG0380016407.01	MsG0580025458.01	0.822601	2.170414e-53	1.825316e-50
MsG0380016505.01	MsG0580025458.01	0.812551	4.005088e-51	2.554166e-48
MsG0380016737.01	MsG0580025458.01	0.823905	1.076818e-53	9.395212e-51
MsG0380016740.01	MsG0580025458.01	0.833939	3.998687e-56	4.675166e-53
MsG0380016923.01	MsG0580025458.01	0.817212	3.707148e-52	2.683082e-49
MsG0380017201.01	MsG0580025458.01	0.816934	4.280534e-52	3.074748e-49
MsG0380017244.01	MsG0580025458.01	0.819383	1.195588e-52	9.185697e-50
MsG0380017401.01	MsG0580025458.01	0.825686	4.093447e-54	3.757802e-51
MsG0380017433.01	MsG0580025458.01	0.805660	1.199229e-49	6.380914e-47
MsG0380017498.01	MsG0580025458.01	0.808066	3.716680e-50	2.104406e-47
MsG0380017528.01	MsG0580025458.01	0.803625	3.188369e-49	1.609937e-46
MsG0380017589.01	MsG0580025458.01	0.842072	3.231550e-58	4.843673e-55
MsG0380018022.01	MsG0580025458.01	0.801981	6.966975e-49	3.373083e-46
MsG0480018402.01	MsG0580025458.01	0.812073	5.092322e-51	3.206018e-48
MsG0280006461.01	MsG0580025458.01	0.820833	5.565354e-53	4.452343e-50
MsG0280006601.01	MsG0580025458.01	0.827573	1.451707e-54	1.406867e-51
MsG0280006679.01	MsG0580025458.01	0.801807	7.563084e-49	3.645751e-46
MsG0280006930.01	MsG0580025458.01	0.802404	5.700117e-49	2.789931e-46
MsG0280007480.01	MsG0580025458.01	0.812551	4.005088e-51	2.554166e-48
MsG0280007496.01	MsG0580025458.01	0.816606	5.070822e-52	3.609691e-49
MsG0280007531.01	MsG0580025458.01	0.806307	8.763882e-50	4.741010e-47
MsG0280007903.01	MsG0580025458.01	0.806624	7.513328e-50	4.097276e-47
MsG0280008100.01	MsG0580025458.01	0.834504	2.885219e-56	3.430934e-53
MsG0280008113.01	MsG0580025458.01	0.808148	3.571690e-50	2.026736e-47
MsG0280008234.01	MsG0580025458.01	0.801283	9.688018e-49	4.608612e-46
MsG0280008257.01	MsG0580025458.01	0.816834	4.506924e-52	3.228374e-49
MsG0280008363.01	MsG0580025458.01	0.820855	5.501883e-53	4.404281e-50
MsG0280008738.01	MsG0580025458.01	0.815284	1.000454e-51	6.868622e-49
MsG0280008765.01	MsG0580025458.01	0.831461	1.647359e-55	1.788972e-52
MsG0280008932.01	MsG0580025458.01	0.802949	4.400568e-49	2.183796e-46
MsG0280010109.01	MsG0580025458.01	0.845105	4.997383e-59	8.239257e-56
MsG0280010158.01	MsG0580025458.01	0.852019	6.074961e-61	1.252392e-57
MsG0280010184.01	MsG0580025458.01	0.829300	5.561838e-55	5.670502e-52
MsG0280010349.01	MsG0580025458.01	0.830383	3.029033e-55	3.186246e-52
MsG0280010350.01	MsG0580025458.01	0.851187	1.044898e-60	2.097929e-57
MsG0280010411.01	MsG0580025458.01	0.802991	4.313399e-49	2.142807e-46
MsG0280010517.01	MsG0580025458.01	0.817120	3.887672e-52	2.806593e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025458.01.T01	MTR_0004s0610	92.063	189	15	0	1	189	154	342	1.07e-126	363
MsG0580025458.01.T01	MTR_1g051205	91.979	187	15	0	1	187	153	339	1.48e-125	360
MsG0580025458.01.T01	MTR_5g068170	86.096	187	9	1	1	187	83	252	7.16e-104	301
MsG0580025458.01.T01	MTR_6g034745	72.340	47	13	0	143	189	271	317	2.62e-16	75.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025458.01.T01	AT3G42630	64.583	192	68	0	1	192	171	362	1.18e-86	261
MsG0580025458.01.T01	AT4G14190	31.928	166	111	2	1	165	256	420	3.07e-14	70.9

Find 38 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAATTTCATTACCTGTTT+TGG	0.192464	5:+19021150	None:intergenic
TTTCGAGAATGGCGCTGTTT+TGG	0.201918	5:-19020805	MsG0580025458.01.T01:CDS
TAGAAGGCTTGGAAGGAATT+TGG	0.229388	5:-19020693	MsG0580025458.01.T01:CDS
GTTCCTGATTTGGTTACTTA+TGG	0.229795	5:-19020740	MsG0580025458.01.T01:CDS
GAAGAGAAAATTCTATGAAT+TGG	0.243285	5:-19021008	MsG0580025458.01.T01:CDS
TTTGTTAGAATGGTTGAATT+AGG	0.246590	5:-19020872	MsG0580025458.01.T01:CDS
TGAGAAGGTGGTTCCTGATT+TGG	0.298398	5:-19020750	MsG0580025458.01.T01:CDS
AATGAAATGGAAAATGCTTA+TGG	0.317533	5:-19021100	MsG0580025458.01.T01:CDS
GATTGATGAAAATGTGATTA+GGG	0.338061	5:-19021053	MsG0580025458.01.T01:CDS
TTGTTAGAATGGTTGAATTA+GGG	0.352168	5:-19020871	MsG0580025458.01.T01:CDS
TTTCAAATAGATTCCAAAAC+AGG	0.356714	5:-19021163	MsG0580025458.01.T01:CDS
GGTCAGTTTGTTAGAGATGT+TGG	0.368673	5:-19020986	MsG0580025458.01.T01:CDS
ATGGAAGGTTTAAGAGATCT+AGG	0.371453	5:-19021081	MsG0580025458.01.T01:CDS
GCAGAGAGAGTTTGTTAGAA+TGG	0.371832	5:-19020882	MsG0580025458.01.T01:CDS
TGATTGATGAAAATGTGATT+AGG	0.372675	5:-19021054	MsG0580025458.01.T01:CDS
GGTTTAGGTAGGAGAAATGT+AGG	0.379552	5:-19020965	MsG0580025458.01.T01:CDS
TTTGTTAGAGATGTTGGTTT+AGG	0.396561	5:-19020980	MsG0580025458.01.T01:CDS
GACGTTTAATATTCGCGCTT+TGG	0.397317	5:-19020831	MsG0580025458.01.T01:CDS
TGGATGCTTACTTGGATAGA+AGG	0.398737	5:-19020709	MsG0580025458.01.T01:CDS
CAACCATAAGTAACCAAATC+AGG	0.419881	5:+19020737	None:intergenic
AAGAGAAAATTCTATGAATT+GGG	0.420495	5:-19021007	MsG0580025458.01.T01:CDS
TTGTGTTGTGGATGCTTACT+TGG	0.434412	5:-19020717	MsG0580025458.01.T01:CDS
ATCAATGAAATGATTTCAAA+GGG	0.437570	5:-19021187	MsG0580025458.01.T01:CDS
ATATTAAACGTCGTAACATC+AGG	0.448111	5:+19020842	None:intergenic
GACGGATCCGTTTGTGTTTG+AGG	0.453067	5:-19020623	None:intergenic
AATCAATGAAATGATTTCAA+AGG	0.463491	5:-19021188	MsG0580025458.01.T01:CDS
GCTTACTTGGATAGAAGGCT+TGG	0.482566	5:-19020704	MsG0580025458.01.T01:CDS
TTAGAGATGTTGGTTTAGGT+AGG	0.503038	5:-19020976	MsG0580025458.01.T01:CDS
CCTAAAACCTCAAACACAAA+CGG	0.528045	5:+19020616	None:intergenic
TGAAAATGTGATTAGGGCTA+TGG	0.528263	5:-19021047	MsG0580025458.01.T01:CDS
AAATGGAAAATGCTTATGGA+AGG	0.541034	5:-19021096	MsG0580025458.01.T01:CDS
GATTTGTCCACAGTTGTTGA+CGG	0.541478	5:-19020641	MsG0580025458.01.T01:CDS
GGTTACTTATGGTTGTGTTG+TGG	0.542271	5:-19020729	MsG0580025458.01.T01:CDS
ACTTGGATAGAAGGCTTGGA+AGG	0.547884	5:-19020700	MsG0580025458.01.T01:CDS
GTTTAGGTAGGAGAAATGTA+GGG	0.576945	5:-19020964	MsG0580025458.01.T01:CDS
TGAACATATGAGAATTGAGA+AGG	0.590282	5:-19020765	MsG0580025458.01.T01:CDS
AACGGATCCGTCAACAACTG+TGG	0.629929	5:+19020634	None:intergenic
ACATATGAGAATTGAGAAGG+TGG	0.658498	5:-19020762	MsG0580025458.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGAGAAAATTCTATGAATT+GGG	-	Chr5:19020817-19020836	MsG0580025458.01.T01:CDS	20.0%
!!	AATCAATGAAATGATTTCAA+AGG	-	Chr5:19020636-19020655	MsG0580025458.01.T01:CDS	20.0%
!!	ATCAATGAAATGATTTCAAA+GGG	-	Chr5:19020637-19020656	MsG0580025458.01.T01:CDS	20.0%
!	AATGAAATGGAAAATGCTTA+TGG	-	Chr5:19020724-19020743	MsG0580025458.01.T01:CDS	25.0%
!	GAAGAGAAAATTCTATGAAT+TGG	-	Chr5:19020816-19020835	MsG0580025458.01.T01:CDS	25.0%
!	GATTGATGAAAATGTGATTA+GGG	-	Chr5:19020771-19020790	MsG0580025458.01.T01:CDS	25.0%
!	TGATTGATGAAAATGTGATT+AGG	-	Chr5:19020770-19020789	MsG0580025458.01.T01:CDS	25.0%
!	TTGTTAGAATGGTTGAATTA+GGG	-	Chr5:19020953-19020972	MsG0580025458.01.T01:CDS	25.0%
!	TTTCAAATAGATTCCAAAAC+AGG	-	Chr5:19020661-19020680	MsG0580025458.01.T01:CDS	25.0%
!	TTTGTTAGAATGGTTGAATT+AGG	-	Chr5:19020952-19020971	MsG0580025458.01.T01:CDS	25.0%
!!	AAGAATTTCATTACCTGTTT+TGG	+	Chr5:19020677-19020696	None:intergenic	25.0%
!!!	GGAGTTTGTTTTGAATAAAA+TGG	-	Chr5:19021152-19021171	MsG0580025458.01.T01:CDS	25.0%
!!!	TGTTTTGAATAAAATGGATG+TGG	-	Chr5:19021158-19021177	MsG0580025458.01.T01:CDS	25.0%
!!!	TTTTGGTAGTTTGAATGAAA+TGG	-	Chr5:19020711-19020730	MsG0580025458.01.T01:CDS	25.0%
	AAATGGAAAATGCTTATGGA+AGG	-	Chr5:19020728-19020747	MsG0580025458.01.T01:CDS	30.0%
	ATATTAAACGTCGTAACATC+AGG	+	Chr5:19020985-19021004	None:intergenic	30.0%
	TGAACATATGAGAATTGAGA+AGG	-	Chr5:19021059-19021078	MsG0580025458.01.T01:CDS	30.0%
!	CTTCTGTGTTATAGTGTTTT+TGG	-	Chr5:19020694-19020713	MsG0580025458.01.T01:CDS	30.0%
!!	TTTGTTAGAGATGTTGGTTT+AGG	-	Chr5:19020844-19020863	MsG0580025458.01.T01:CDS	30.0%
	ACATATGAGAATTGAGAAGG+TGG	-	Chr5:19021062-19021081	MsG0580025458.01.T01:CDS	35.0%
	ATGGAAGGTTTAAGAGATCT+AGG	-	Chr5:19020743-19020762	MsG0580025458.01.T01:CDS	35.0%
	CAACCATAAGTAACCAAATC+AGG	+	Chr5:19021090-19021109	None:intergenic	35.0%
	GTTTAGGTAGGAGAAATGTA+GGG	-	Chr5:19020860-19020879	MsG0580025458.01.T01:CDS	35.0%
	TGAAAATGTGATTAGGGCTA+TGG	-	Chr5:19020777-19020796	MsG0580025458.01.T01:CDS	35.0%
!	GAAATGTAGGGAATCTTTTG+TGG	-	Chr5:19020872-19020891	MsG0580025458.01.T01:CDS	35.0%
!!	GTTCCTGATTTGGTTACTTA+TGG	-	Chr5:19021084-19021103	MsG0580025458.01.T01:CDS	35.0%
!!	TTAGAGATGTTGGTTTAGGT+AGG	-	Chr5:19020848-19020867	MsG0580025458.01.T01:CDS	35.0%
	GACGTTTAATATTCGCGCTT+TGG	-	Chr5:19020993-19021012	MsG0580025458.01.T01:CDS	40.0%
	GATTTGTCCACAGTTGTTGA+CGG	-	Chr5:19021183-19021202	MsG0580025458.01.T01:CDS	40.0%
	GGTTACTTATGGTTGTGTTG+TGG	-	Chr5:19021095-19021114	MsG0580025458.01.T01:CDS	40.0%
	GGTTTAGGTAGGAGAAATGT+AGG	-	Chr5:19020859-19020878	MsG0580025458.01.T01:CDS	40.0%
	TAGAAGGCTTGGAAGGAATT+TGG	-	Chr5:19021131-19021150	MsG0580025458.01.T01:CDS	40.0%
	TGGATGCTTACTTGGATAGA+AGG	-	Chr5:19021115-19021134	MsG0580025458.01.T01:CDS	40.0%
	TTGTGTTGTGGATGCTTACT+TGG	-	Chr5:19021107-19021126	MsG0580025458.01.T01:CDS	40.0%
!!	GCAGAGAGAGTTTGTTAGAA+TGG	-	Chr5:19020942-19020961	MsG0580025458.01.T01:CDS	40.0%
!!	GGTCAGTTTGTTAGAGATGT+TGG	-	Chr5:19020838-19020857	MsG0580025458.01.T01:CDS	40.0%
	ACTTGGATAGAAGGCTTGGA+AGG	-	Chr5:19021124-19021143	MsG0580025458.01.T01:CDS	45.0%
	GCTTACTTGGATAGAAGGCT+TGG	-	Chr5:19021120-19021139	MsG0580025458.01.T01:CDS	45.0%
	TGAGAAGGTGGTTCCTGATT+TGG	-	Chr5:19021074-19021093	MsG0580025458.01.T01:CDS	45.0%
!	TTTCGAGAATGGCGCTGTTT+TGG	-	Chr5:19021019-19021038	MsG0580025458.01.T01:CDS	45.0%
!!	TTCGAGAATGGCGCTGTTTT+GGG	-	Chr5:19021020-19021039	MsG0580025458.01.T01:CDS	45.0%
	AACGGATCCGTCAACAACTG+TGG	+	Chr5:19021193-19021212	None:intergenic	50.0%
!!	CGCTTTGGCGTTTTCGAGAA+TGG	-	Chr5:19021008-19021027	MsG0580025458.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	19020625	19021221	19020625	ID=MsG0580025458.01;Name=MsG0580025458.01
Chr5	mRNA	19020625	19021221	19020625	ID=MsG0580025458.01.T01;Parent=MsG0580025458.01;Name=MsG0580025458.01.T01;_AED=0.36;_eAED=0.36;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|198
Chr5	exon	19020625	19021221	19020625	ID=MsG0580025458.01.T01:exon:9779;Parent=MsG0580025458.01.T01
Chr5	CDS	19020625	19021221	19020625	ID=MsG0580025458.01.T01:cds;Parent=MsG0580025458.01.T01

Gene Sequence

>MsG0580025458.01.T01

ATGGAAGAAACAATCAATGAAATGATTTCAAAGGGTTTTCAAATAGATTCCAAAACAGGTAATGAAATTCTTCTGTGTTATAGTGTTTTTGGTAGTTTGAATGAAATGGAAAATGCTTATGGAAGGTTTAAGAGATCTAGGTTTTTGATTGATGAAAATGTGATTAGGGCTATGGCTTATGCTTATATAAAGAAGAGAAAATTCTATGAATTGGGTCAGTTTGTTAGAGATGTTGGTTTAGGTAGGAGAAATGTAGGGAATCTTTTGTGGAATCTCTTATTGCTTTCTTATGCTGCTAATTTTAAGATGAAGAGTTTGCAGAGAGAGTTTGTTAGAATGGTTGAATTAGGGTTTCGTCCTGATGTTACGACGTTTAATATTCGCGCTTTGGCGTTTTCGAGAATGGCGCTGTTTTGGGATCTTCATCTTAGTATTGAACATATGAGAATTGAGAAGGTGGTTCCTGATTTGGTTACTTATGGTTGTGTTGTGGATGCTTACTTGGATAGAAGGCTTGGAAGGAATTTGGAGTTTGTTTTGAATAAAATGGATGTGGATGATTTGTCCACAGTTGTTGACGGATCCGTTTGTGTTTGA

Protein sequence

>MsG0580025458.01.T01

MEETINEMISKGFQIDSKTGNEILLCYSVFGSLNEMENAYGRFKRSRFLIDENVIRAMAYAYIKKRKFYELGQFVRDVGLGRRNVGNLLWNLLLLSYAANFKMKSLQREFVRMVELGFRPDVTTFNIRALAFSRMALFWDLHLSIEHMRIEKVVPDLVTYGCVVDAYLDRRLGRNLEFVLNKMDVDDLSTVVDGSVCV*