AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028175.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028175.01.T01 MTR_0004s0610 92.063 189 15 0 1 189 154 342 1.07e-126 363
MsG0580028175.01.T01 MTR_1g051205 91.979 187 15 0 1 187 153 339 1.48e-125 360
MsG0580028175.01.T01 MTR_5g068170 86.096 187 9 1 1 187 83 252 7.16e-104 301
MsG0580028175.01.T01 MTR_6g034745 72.340 47 13 0 143 189 271 317 2.62e-16 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028175.01.T01 AT3G42630 64.583 192 68 0 1 192 171 362 1.18e-86 261
MsG0580028175.01.T01 AT4G14190 31.928 166 111 2 1 165 256 420 3.07e-14 70.9

Find 38 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAATTTCATTACCTGTTT+TGG 0.192464 5:-76177827 None:intergenic
TTTCGAGAATGGCGCTGTTT+TGG 0.201918 5:+76178172 MsG0580028175.01.T01:CDS
TAGAAGGCTTGGAAGGAATT+TGG 0.229388 5:+76178284 MsG0580028175.01.T01:CDS
GTTCCTGATTTGGTTACTTA+TGG 0.229795 5:+76178237 MsG0580028175.01.T01:CDS
GAAGAGAAAATTCTATGAAT+TGG 0.243285 5:+76177969 MsG0580028175.01.T01:CDS
TTTGTTAGAATGGTTGAATT+AGG 0.246590 5:+76178105 MsG0580028175.01.T01:CDS
TGAGAAGGTGGTTCCTGATT+TGG 0.298398 5:+76178227 MsG0580028175.01.T01:CDS
AATGAAATGGAAAATGCTTA+TGG 0.317533 5:+76177877 MsG0580028175.01.T01:CDS
GATTGATGAAAATGTGATTA+GGG 0.338061 5:+76177924 MsG0580028175.01.T01:CDS
TTGTTAGAATGGTTGAATTA+GGG 0.352168 5:+76178106 MsG0580028175.01.T01:CDS
TTTCAAATAGATTCCAAAAC+AGG 0.356714 5:+76177814 MsG0580028175.01.T01:CDS
GGTCAGTTTGTTAGAGATGT+TGG 0.368673 5:+76177991 MsG0580028175.01.T01:CDS
ATGGAAGGTTTAAGAGATCT+AGG 0.371453 5:+76177896 MsG0580028175.01.T01:CDS
GCAGAGAGAGTTTGTTAGAA+TGG 0.371832 5:+76178095 MsG0580028175.01.T01:CDS
TGATTGATGAAAATGTGATT+AGG 0.372675 5:+76177923 MsG0580028175.01.T01:CDS
GGTTTAGGTAGGAGAAATGT+AGG 0.379552 5:+76178012 MsG0580028175.01.T01:CDS
TTTGTTAGAGATGTTGGTTT+AGG 0.396561 5:+76177997 MsG0580028175.01.T01:CDS
GACGTTTAATATTCGCGCTT+TGG 0.397317 5:+76178146 MsG0580028175.01.T01:CDS
TGGATGCTTACTTGGATAGA+AGG 0.398737 5:+76178268 MsG0580028175.01.T01:CDS
CAACCATAAGTAACCAAATC+AGG 0.419881 5:-76178240 None:intergenic
AAGAGAAAATTCTATGAATT+GGG 0.420495 5:+76177970 MsG0580028175.01.T01:CDS
TTGTGTTGTGGATGCTTACT+TGG 0.434412 5:+76178260 MsG0580028175.01.T01:CDS
ATCAATGAAATGATTTCAAA+GGG 0.437570 5:+76177790 MsG0580028175.01.T01:CDS
ATATTAAACGTCGTAACATC+AGG 0.448111 5:-76178135 None:intergenic
GACGGATCCGTTTGTGTTTG+AGG 0.453067 5:+76178354 MsG0580028175.01.T01:CDS
AATCAATGAAATGATTTCAA+AGG 0.463491 5:+76177789 MsG0580028175.01.T01:CDS
GCTTACTTGGATAGAAGGCT+TGG 0.482566 5:+76178273 MsG0580028175.01.T01:CDS
TTAGAGATGTTGGTTTAGGT+AGG 0.503038 5:+76178001 MsG0580028175.01.T01:CDS
CCTAAAACCTCAAACACAAA+CGG 0.528045 5:-76178361 None:intergenic
TGAAAATGTGATTAGGGCTA+TGG 0.528263 5:+76177930 MsG0580028175.01.T01:CDS
AAATGGAAAATGCTTATGGA+AGG 0.541034 5:+76177881 MsG0580028175.01.T01:CDS
GATTTGTCCACAGTTGTTGA+CGG 0.541478 5:+76178336 MsG0580028175.01.T01:CDS
GGTTACTTATGGTTGTGTTG+TGG 0.542271 5:+76178248 MsG0580028175.01.T01:CDS
ACTTGGATAGAAGGCTTGGA+AGG 0.547884 5:+76178277 MsG0580028175.01.T01:CDS
GTTTAGGTAGGAGAAATGTA+GGG 0.576945 5:+76178013 MsG0580028175.01.T01:CDS
TGAACATATGAGAATTGAGA+AGG 0.590282 5:+76178212 MsG0580028175.01.T01:CDS
AACGGATCCGTCAACAACTG+TGG 0.629929 5:-76178343 None:intergenic
ACATATGAGAATTGAGAAGG+TGG 0.658498 5:+76178215 MsG0580028175.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAGAAAATTCTATGAATT+GGG + Chr5:76177970-76177989 MsG0580028175.01.T01:CDS 20.0%
!! AATCAATGAAATGATTTCAA+AGG + Chr5:76177789-76177808 MsG0580028175.01.T01:CDS 20.0%
!! ATCAATGAAATGATTTCAAA+GGG + Chr5:76177790-76177809 MsG0580028175.01.T01:CDS 20.0%
! AATGAAATGGAAAATGCTTA+TGG + Chr5:76177877-76177896 MsG0580028175.01.T01:CDS 25.0%
! GAAGAGAAAATTCTATGAAT+TGG + Chr5:76177969-76177988 MsG0580028175.01.T01:CDS 25.0%
! GATTGATGAAAATGTGATTA+GGG + Chr5:76177924-76177943 MsG0580028175.01.T01:CDS 25.0%
! TGATTGATGAAAATGTGATT+AGG + Chr5:76177923-76177942 MsG0580028175.01.T01:CDS 25.0%
! TTGTTAGAATGGTTGAATTA+GGG + Chr5:76178106-76178125 MsG0580028175.01.T01:CDS 25.0%
! TTTCAAATAGATTCCAAAAC+AGG + Chr5:76177814-76177833 MsG0580028175.01.T01:CDS 25.0%
! TTTGTTAGAATGGTTGAATT+AGG + Chr5:76178105-76178124 MsG0580028175.01.T01:CDS 25.0%
!! AAGAATTTCATTACCTGTTT+TGG - Chr5:76177830-76177849 None:intergenic 25.0%
!!! GGAGTTTGTTTTGAATAAAA+TGG + Chr5:76178305-76178324 MsG0580028175.01.T01:CDS 25.0%
!!! TGTTTTGAATAAAATGGATG+TGG + Chr5:76178311-76178330 MsG0580028175.01.T01:CDS 25.0%
!!! TTTTGGTAGTTTGAATGAAA+TGG + Chr5:76177864-76177883 MsG0580028175.01.T01:CDS 25.0%
AAATGGAAAATGCTTATGGA+AGG + Chr5:76177881-76177900 MsG0580028175.01.T01:CDS 30.0%
ATATTAAACGTCGTAACATC+AGG - Chr5:76178138-76178157 None:intergenic 30.0%
TGAACATATGAGAATTGAGA+AGG + Chr5:76178212-76178231 MsG0580028175.01.T01:CDS 30.0%
! CTTCTGTGTTATAGTGTTTT+TGG + Chr5:76177847-76177866 MsG0580028175.01.T01:CDS 30.0%
!! TTTGTTAGAGATGTTGGTTT+AGG + Chr5:76177997-76178016 MsG0580028175.01.T01:CDS 30.0%
ACATATGAGAATTGAGAAGG+TGG + Chr5:76178215-76178234 MsG0580028175.01.T01:CDS 35.0%
ATGGAAGGTTTAAGAGATCT+AGG + Chr5:76177896-76177915 MsG0580028175.01.T01:CDS 35.0%
CAACCATAAGTAACCAAATC+AGG - Chr5:76178243-76178262 None:intergenic 35.0%
GTTTAGGTAGGAGAAATGTA+GGG + Chr5:76178013-76178032 MsG0580028175.01.T01:CDS 35.0%
TGAAAATGTGATTAGGGCTA+TGG + Chr5:76177930-76177949 MsG0580028175.01.T01:CDS 35.0%
! GAAATGTAGGGAATCTTTTG+TGG + Chr5:76178025-76178044 MsG0580028175.01.T01:CDS 35.0%
!! GTTCCTGATTTGGTTACTTA+TGG + Chr5:76178237-76178256 MsG0580028175.01.T01:CDS 35.0%
!! TTAGAGATGTTGGTTTAGGT+AGG + Chr5:76178001-76178020 MsG0580028175.01.T01:CDS 35.0%
GACGTTTAATATTCGCGCTT+TGG + Chr5:76178146-76178165 MsG0580028175.01.T01:CDS 40.0%
GATTTGTCCACAGTTGTTGA+CGG + Chr5:76178336-76178355 MsG0580028175.01.T01:CDS 40.0%
GGTTACTTATGGTTGTGTTG+TGG + Chr5:76178248-76178267 MsG0580028175.01.T01:CDS 40.0%
GGTTTAGGTAGGAGAAATGT+AGG + Chr5:76178012-76178031 MsG0580028175.01.T01:CDS 40.0%
TAGAAGGCTTGGAAGGAATT+TGG + Chr5:76178284-76178303 MsG0580028175.01.T01:CDS 40.0%
TGGATGCTTACTTGGATAGA+AGG + Chr5:76178268-76178287 MsG0580028175.01.T01:CDS 40.0%
TTGTGTTGTGGATGCTTACT+TGG + Chr5:76178260-76178279 MsG0580028175.01.T01:CDS 40.0%
!! GCAGAGAGAGTTTGTTAGAA+TGG + Chr5:76178095-76178114 MsG0580028175.01.T01:CDS 40.0%
!! GGTCAGTTTGTTAGAGATGT+TGG + Chr5:76177991-76178010 MsG0580028175.01.T01:CDS 40.0%
ACTTGGATAGAAGGCTTGGA+AGG + Chr5:76178277-76178296 MsG0580028175.01.T01:CDS 45.0%
GCTTACTTGGATAGAAGGCT+TGG + Chr5:76178273-76178292 MsG0580028175.01.T01:CDS 45.0%
TGAGAAGGTGGTTCCTGATT+TGG + Chr5:76178227-76178246 MsG0580028175.01.T01:CDS 45.0%
! TTTCGAGAATGGCGCTGTTT+TGG + Chr5:76178172-76178191 MsG0580028175.01.T01:CDS 45.0%
!! TTCGAGAATGGCGCTGTTTT+GGG + Chr5:76178173-76178192 MsG0580028175.01.T01:CDS 45.0%
AACGGATCCGTCAACAACTG+TGG - Chr5:76178346-76178365 None:intergenic 50.0%
!! CGCTTTGGCGTTTTCGAGAA+TGG + Chr5:76178161-76178180 MsG0580028175.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr5 gene 76177778 76178374 76177778 ID=MsG0580028175.01;Name=MsG0580028175.01
Chr5 mRNA 76177778 76178374 76177778 ID=MsG0580028175.01.T01;Parent=MsG0580028175.01;Name=MsG0580028175.01.T01;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|198
Chr5 exon 76177778 76178374 76177778 ID=MsG0580028175.01.T01:exon:9780;Parent=MsG0580028175.01.T01
Chr5 CDS 76177778 76178374 76177778 ID=MsG0580028175.01.T01:cds;Parent=MsG0580028175.01.T01
Gene Sequence

>MsG0580028175.01.T01

ATGGAAGAAACAATCAATGAAATGATTTCAAAGGGTTTTCAAATAGATTCCAAAACAGGTAATGAAATTCTTCTGTGTTATAGTGTTTTTGGTAGTTTGAATGAAATGGAAAATGCTTATGGAAGGTTTAAGAGATCTAGGTTTTTGATTGATGAAAATGTGATTAGGGCTATGGCTTATGCTTATATAAAGAAGAGAAAATTCTATGAATTGGGTCAGTTTGTTAGAGATGTTGGTTTAGGTAGGAGAAATGTAGGGAATCTTTTGTGGAATCTCTTATTGCTTTCTTATGCTGCTAATTTTAAGATGAAGAGTTTGCAGAGAGAGTTTGTTAGAATGGTTGAATTAGGGTTTCGTCCTGATGTTACGACGTTTAATATTCGCGCTTTGGCGTTTTCGAGAATGGCGCTGTTTTGGGATCTTCATCTTAGTATTGAACATATGAGAATTGAGAAGGTGGTTCCTGATTTGGTTACTTATGGTTGTGTTGTGGATGCTTACTTGGATAGAAGGCTTGGAAGGAATTTGGAGTTTGTTTTGAATAAAATGGATGTGGATGATTTGTCCACAGTTGTTGACGGATCCGTTTGTGTTTGA

Protein sequence

>MsG0580028175.01.T01

MEETINEMISKGFQIDSKTGNEILLCYSVFGSLNEMENAYGRFKRSRFLIDENVIRAMAYAYIKKRKFYELGQFVRDVGLGRRNVGNLLWNLLLLSYAANFKMKSLQREFVRMVELGFRPDVTTFNIRALAFSRMALFWDLHLSIEHMRIEKVVPDLVTYGCVVDAYLDRRLGRNLEFVLNKMDVDDLSTVVDGSVCV*