AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025460.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025460.01.T01 MTR_0004s0610 92.063 189 15 0 1 189 154 342 1.07e-126 363
MsG0580025460.01.T01 MTR_1g051205 91.979 187 15 0 1 187 153 339 1.48e-125 360
MsG0580025460.01.T01 MTR_5g068170 86.096 187 9 1 1 187 83 252 7.16e-104 301
MsG0580025460.01.T01 MTR_6g034745 72.340 47 13 0 143 189 271 317 2.62e-16 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025460.01.T01 AT3G42630 64.583 192 68 0 1 192 171 362 1.18e-86 261
MsG0580025460.01.T01 AT4G14190 31.928 166 111 2 1 165 256 420 3.07e-14 70.9

Find 38 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAATTTCATTACCTGTTT+TGG 0.192464 5:+19045872 None:intergenic
TTTCGAGAATGGCGCTGTTT+TGG 0.201918 5:-19045527 MsG0580025460.01.T01:CDS
TAGAAGGCTTGGAAGGAATT+TGG 0.229388 5:-19045415 MsG0580025460.01.T01:CDS
GTTCCTGATTTGGTTACTTA+TGG 0.229795 5:-19045462 MsG0580025460.01.T01:CDS
GAAGAGAAAATTCTATGAAT+TGG 0.243285 5:-19045730 MsG0580025460.01.T01:CDS
TTTGTTAGAATGGTTGAATT+AGG 0.246590 5:-19045594 MsG0580025460.01.T01:CDS
TGAGAAGGTGGTTCCTGATT+TGG 0.298398 5:-19045472 MsG0580025460.01.T01:CDS
AATGAAATGGAAAATGCTTA+TGG 0.317533 5:-19045822 MsG0580025460.01.T01:CDS
GATTGATGAAAATGTGATTA+GGG 0.338061 5:-19045775 MsG0580025460.01.T01:CDS
TTGTTAGAATGGTTGAATTA+GGG 0.352168 5:-19045593 MsG0580025460.01.T01:CDS
TTTCAAATAGATTCCAAAAC+AGG 0.356714 5:-19045885 MsG0580025460.01.T01:CDS
GGTCAGTTTGTTAGAGATGT+TGG 0.368673 5:-19045708 MsG0580025460.01.T01:CDS
ATGGAAGGTTTAAGAGATCT+AGG 0.371453 5:-19045803 MsG0580025460.01.T01:CDS
GCAGAGAGAGTTTGTTAGAA+TGG 0.371832 5:-19045604 MsG0580025460.01.T01:CDS
TGATTGATGAAAATGTGATT+AGG 0.372675 5:-19045776 MsG0580025460.01.T01:CDS
GGTTTAGGTAGGAGAAATGT+AGG 0.379552 5:-19045687 MsG0580025460.01.T01:CDS
TTTGTTAGAGATGTTGGTTT+AGG 0.396561 5:-19045702 MsG0580025460.01.T01:CDS
GACGTTTAATATTCGCGCTT+TGG 0.397317 5:-19045553 MsG0580025460.01.T01:CDS
TGGATGCTTACTTGGATAGA+AGG 0.398737 5:-19045431 MsG0580025460.01.T01:CDS
CAACCATAAGTAACCAAATC+AGG 0.419881 5:+19045459 None:intergenic
AAGAGAAAATTCTATGAATT+GGG 0.420495 5:-19045729 MsG0580025460.01.T01:CDS
TTGTGTTGTGGATGCTTACT+TGG 0.434412 5:-19045439 MsG0580025460.01.T01:CDS
ATCAATGAAATGATTTCAAA+GGG 0.437570 5:-19045909 MsG0580025460.01.T01:CDS
ATATTAAACGTCGTAACATC+AGG 0.448111 5:+19045564 None:intergenic
GACGGATCCGTTTGTGTTTG+AGG 0.453067 5:-19045345 None:intergenic
AATCAATGAAATGATTTCAA+AGG 0.463491 5:-19045910 MsG0580025460.01.T01:CDS
GCTTACTTGGATAGAAGGCT+TGG 0.482566 5:-19045426 MsG0580025460.01.T01:CDS
TTAGAGATGTTGGTTTAGGT+AGG 0.503038 5:-19045698 MsG0580025460.01.T01:CDS
CCTAAAACCTCAAACACAAA+CGG 0.528045 5:+19045338 None:intergenic
TGAAAATGTGATTAGGGCTA+TGG 0.528263 5:-19045769 MsG0580025460.01.T01:CDS
AAATGGAAAATGCTTATGGA+AGG 0.541034 5:-19045818 MsG0580025460.01.T01:CDS
GATTTGTCCACAGTTGTTGA+CGG 0.541478 5:-19045363 MsG0580025460.01.T01:CDS
GGTTACTTATGGTTGTGTTG+TGG 0.542271 5:-19045451 MsG0580025460.01.T01:CDS
ACTTGGATAGAAGGCTTGGA+AGG 0.547884 5:-19045422 MsG0580025460.01.T01:CDS
GTTTAGGTAGGAGAAATGTA+GGG 0.576945 5:-19045686 MsG0580025460.01.T01:CDS
TGAACATATGAGAATTGAGA+AGG 0.590282 5:-19045487 MsG0580025460.01.T01:CDS
AACGGATCCGTCAACAACTG+TGG 0.629929 5:+19045356 None:intergenic
ACATATGAGAATTGAGAAGG+TGG 0.658498 5:-19045484 MsG0580025460.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAGAAAATTCTATGAATT+GGG - Chr5:19045539-19045558 MsG0580025460.01.T01:CDS 20.0%
!! AATCAATGAAATGATTTCAA+AGG - Chr5:19045358-19045377 MsG0580025460.01.T01:CDS 20.0%
!! ATCAATGAAATGATTTCAAA+GGG - Chr5:19045359-19045378 MsG0580025460.01.T01:CDS 20.0%
! AATGAAATGGAAAATGCTTA+TGG - Chr5:19045446-19045465 MsG0580025460.01.T01:CDS 25.0%
! GAAGAGAAAATTCTATGAAT+TGG - Chr5:19045538-19045557 MsG0580025460.01.T01:CDS 25.0%
! GATTGATGAAAATGTGATTA+GGG - Chr5:19045493-19045512 MsG0580025460.01.T01:CDS 25.0%
! TGATTGATGAAAATGTGATT+AGG - Chr5:19045492-19045511 MsG0580025460.01.T01:CDS 25.0%
! TTGTTAGAATGGTTGAATTA+GGG - Chr5:19045675-19045694 MsG0580025460.01.T01:CDS 25.0%
! TTTCAAATAGATTCCAAAAC+AGG - Chr5:19045383-19045402 MsG0580025460.01.T01:CDS 25.0%
! TTTGTTAGAATGGTTGAATT+AGG - Chr5:19045674-19045693 MsG0580025460.01.T01:CDS 25.0%
!! AAGAATTTCATTACCTGTTT+TGG + Chr5:19045399-19045418 None:intergenic 25.0%
!!! GGAGTTTGTTTTGAATAAAA+TGG - Chr5:19045874-19045893 MsG0580025460.01.T01:CDS 25.0%
!!! TGTTTTGAATAAAATGGATG+TGG - Chr5:19045880-19045899 MsG0580025460.01.T01:CDS 25.0%
!!! TTTTGGTAGTTTGAATGAAA+TGG - Chr5:19045433-19045452 MsG0580025460.01.T01:CDS 25.0%
AAATGGAAAATGCTTATGGA+AGG - Chr5:19045450-19045469 MsG0580025460.01.T01:CDS 30.0%
ATATTAAACGTCGTAACATC+AGG + Chr5:19045707-19045726 None:intergenic 30.0%
TGAACATATGAGAATTGAGA+AGG - Chr5:19045781-19045800 MsG0580025460.01.T01:CDS 30.0%
! CTTCTGTGTTATAGTGTTTT+TGG - Chr5:19045416-19045435 MsG0580025460.01.T01:CDS 30.0%
!! TTTGTTAGAGATGTTGGTTT+AGG - Chr5:19045566-19045585 MsG0580025460.01.T01:CDS 30.0%
ACATATGAGAATTGAGAAGG+TGG - Chr5:19045784-19045803 MsG0580025460.01.T01:CDS 35.0%
ATGGAAGGTTTAAGAGATCT+AGG - Chr5:19045465-19045484 MsG0580025460.01.T01:CDS 35.0%
CAACCATAAGTAACCAAATC+AGG + Chr5:19045812-19045831 None:intergenic 35.0%
GTTTAGGTAGGAGAAATGTA+GGG - Chr5:19045582-19045601 MsG0580025460.01.T01:CDS 35.0%
TGAAAATGTGATTAGGGCTA+TGG - Chr5:19045499-19045518 MsG0580025460.01.T01:CDS 35.0%
! GAAATGTAGGGAATCTTTTG+TGG - Chr5:19045594-19045613 MsG0580025460.01.T01:CDS 35.0%
!! GTTCCTGATTTGGTTACTTA+TGG - Chr5:19045806-19045825 MsG0580025460.01.T01:CDS 35.0%
!! TTAGAGATGTTGGTTTAGGT+AGG - Chr5:19045570-19045589 MsG0580025460.01.T01:CDS 35.0%
GACGTTTAATATTCGCGCTT+TGG - Chr5:19045715-19045734 MsG0580025460.01.T01:CDS 40.0%
GATTTGTCCACAGTTGTTGA+CGG - Chr5:19045905-19045924 MsG0580025460.01.T01:CDS 40.0%
GGTTACTTATGGTTGTGTTG+TGG - Chr5:19045817-19045836 MsG0580025460.01.T01:CDS 40.0%
GGTTTAGGTAGGAGAAATGT+AGG - Chr5:19045581-19045600 MsG0580025460.01.T01:CDS 40.0%
TAGAAGGCTTGGAAGGAATT+TGG - Chr5:19045853-19045872 MsG0580025460.01.T01:CDS 40.0%
TGGATGCTTACTTGGATAGA+AGG - Chr5:19045837-19045856 MsG0580025460.01.T01:CDS 40.0%
TTGTGTTGTGGATGCTTACT+TGG - Chr5:19045829-19045848 MsG0580025460.01.T01:CDS 40.0%
!! GCAGAGAGAGTTTGTTAGAA+TGG - Chr5:19045664-19045683 MsG0580025460.01.T01:CDS 40.0%
!! GGTCAGTTTGTTAGAGATGT+TGG - Chr5:19045560-19045579 MsG0580025460.01.T01:CDS 40.0%
ACTTGGATAGAAGGCTTGGA+AGG - Chr5:19045846-19045865 MsG0580025460.01.T01:CDS 45.0%
GCTTACTTGGATAGAAGGCT+TGG - Chr5:19045842-19045861 MsG0580025460.01.T01:CDS 45.0%
TGAGAAGGTGGTTCCTGATT+TGG - Chr5:19045796-19045815 MsG0580025460.01.T01:CDS 45.0%
! TTTCGAGAATGGCGCTGTTT+TGG - Chr5:19045741-19045760 MsG0580025460.01.T01:CDS 45.0%
!! TTCGAGAATGGCGCTGTTTT+GGG - Chr5:19045742-19045761 MsG0580025460.01.T01:CDS 45.0%
AACGGATCCGTCAACAACTG+TGG + Chr5:19045915-19045934 None:intergenic 50.0%
!! CGCTTTGGCGTTTTCGAGAA+TGG - Chr5:19045730-19045749 MsG0580025460.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr5 gene 19045347 19045943 19045347 ID=MsG0580025460.01;Name=MsG0580025460.01
Chr5 mRNA 19045347 19045943 19045347 ID=MsG0580025460.01.T01;Parent=MsG0580025460.01;Name=MsG0580025460.01.T01;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|198
Chr5 exon 19045347 19045943 19045347 ID=MsG0580025460.01.T01:exon:15429;Parent=MsG0580025460.01.T01
Chr5 CDS 19045347 19045943 19045347 ID=MsG0580025460.01.T01:cds;Parent=MsG0580025460.01.T01
Gene Sequence

>MsG0580025460.01.T01

ATGGAAGAAACAATCAATGAAATGATTTCAAAGGGTTTTCAAATAGATTCCAAAACAGGTAATGAAATTCTTCTGTGTTATAGTGTTTTTGGTAGTTTGAATGAAATGGAAAATGCTTATGGAAGGTTTAAGAGATCTAGGTTTTTGATTGATGAAAATGTGATTAGGGCTATGGCTTATGCTTATATAAAGAAGAGAAAATTCTATGAATTGGGTCAGTTTGTTAGAGATGTTGGTTTAGGTAGGAGAAATGTAGGGAATCTTTTGTGGAATCTCTTATTGCTTTCTTATGCTGCTAATTTTAAGATGAAGAGTTTGCAGAGAGAGTTTGTTAGAATGGTTGAATTAGGGTTTCGTCCTGATGTTACGACGTTTAATATTCGCGCTTTGGCGTTTTCGAGAATGGCGCTGTTTTGGGATCTTCATCTTAGTATTGAACATATGAGAATTGAGAAGGTGGTTCCTGATTTGGTTACTTATGGTTGTGTTGTGGATGCTTACTTGGATAGAAGGCTTGGAAGGAATTTGGAGTTTGTTTTGAATAAAATGGATGTGGATGATTTGTCCACAGTTGTTGACGGATCCGTTTGTGTTTGA

Protein sequence

>MsG0580025460.01.T01

MEETINEMISKGFQIDSKTGNEILLCYSVFGSLNEMENAYGRFKRSRFLIDENVIRAMAYAYIKKRKFYELGQFVRDVGLGRRNVGNLLWNLLLLSYAANFKMKSLQREFVRMVELGFRPDVTTFNIRALAFSRMALFWDLHLSIEHMRIEKVVPDLVTYGCVVDAYLDRRLGRNLEFVLNKMDVDDLSTVVDGSVCV*