Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0236700 | XP_003593896.1 | 95.575 | 113 | 5 | 0 | 1 | 113 | 1 | 113 | 3.26e-72 | 220 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0236700 | G7IP64 | 95.575 | 113 | 5 | 0 | 1 | 113 | 1 | 113 | 1.56e-72 | 220 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000550 | Msa0236700 | 0.802928 | 4.445466e-49 | -8.615850e-47 |
| Msa0014570 | Msa0236700 | -0.800304 | 1.534459e-48 | -8.615850e-47 |
| Msa0022750 | Msa0236700 | 0.831422 | 1.684758e-55 | -8.615850e-47 |
| Msa0029850 | Msa0236700 | -0.814784 | 1.291348e-51 | -8.615850e-47 |
| Msa0032320 | Msa0236700 | -0.806748 | 7.076518e-50 | -8.615850e-47 |
| Msa0042550 | Msa0236700 | -0.839010 | 2.045856e-57 | -8.615850e-47 |
| Msa0050930 | Msa0236700 | -0.812223 | 4.724597e-51 | -8.615850e-47 |
| Msa0055070 | Msa0236700 | -0.831622 | 1.503900e-55 | -8.615850e-47 |
| Msa0078610 | Msa0236700 | -0.832863 | 7.417134e-56 | -8.615850e-47 |
| Msa0085720 | Msa0236700 | -0.804825 | 1.793308e-49 | -8.615850e-47 |
| Msa0101020 | Msa0236700 | -0.829776 | 4.260877e-55 | -8.615850e-47 |
| Msa0101880 | Msa0236700 | -0.817129 | 3.869357e-52 | -8.615850e-47 |
| Msa0124550 | Msa0236700 | -0.834898 | 2.296650e-56 | -8.615850e-47 |
| Msa0140500 | Msa0236700 | 0.804429 | 2.169507e-49 | -8.615850e-47 |
| Msa0140510 | Msa0236700 | 0.831240 | 1.867014e-55 | -8.615850e-47 |
| Msa0144790 | Msa0236700 | -0.804755 | 1.854844e-49 | -8.615850e-47 |
| Msa0145110 | Msa0236700 | -0.815013 | 1.148714e-51 | -8.615850e-47 |
| Msa0154440 | Msa0236700 | -0.801074 | 1.068978e-48 | -8.615850e-47 |
| Msa0162790 | Msa0236700 | 0.828082 | 1.095691e-54 | -8.615850e-47 |
| Msa0169820 | Msa0236700 | -0.828554 | 8.428992e-55 | -8.615850e-47 |
| Msa0178510 | Msa0236700 | -0.853334 | 2.559080e-61 | -8.615850e-47 |
| Msa0183010 | Msa0236700 | 0.847613 | 1.034978e-59 | -8.615850e-47 |
| Msa0187030 | Msa0236700 | -0.802165 | 6.386757e-49 | -8.615850e-47 |
| Msa0188050 | Msa0236700 | -0.802279 | 6.050225e-49 | -8.615850e-47 |
| Msa0199800 | Msa0236700 | 0.837225 | 5.892356e-57 | -8.615850e-47 |
| Msa0213040 | Msa0236700 | -0.837225 | 5.892096e-57 | -8.615850e-47 |
| Msa0223410 | Msa0236700 | 0.818172 | 2.251484e-52 | -8.615850e-47 |
| Msa0227230 | Msa0236700 | -0.809619 | 1.729985e-50 | -8.615850e-47 |
| Msa0230640 | Msa0236700 | 0.822126 | 2.799140e-53 | -8.615850e-47 |
| Msa0236700 | Msa0238230 | -0.805864 | 1.086707e-49 | -8.615850e-47 |
| Msa0236700 | Msa0239690 | -0.801780 | 7.659868e-49 | -8.615850e-47 |
| Msa0236700 | Msa0243250 | -0.803964 | 2.710791e-49 | -8.615850e-47 |
| Msa0236700 | Msa0249560 | -0.806964 | 6.369627e-50 | -8.615850e-47 |
| Msa0236700 | Msa0250670 | -0.800726 | 1.258832e-48 | -8.615850e-47 |
| Msa0236700 | Msa0253340 | -0.826849 | 2.163994e-54 | -8.615850e-47 |
| Msa0236700 | Msa0266240 | -0.819500 | 1.124238e-52 | -8.615850e-47 |
| Msa0236700 | Msa0268090 | -0.809244 | 2.082532e-50 | -8.615850e-47 |
| Msa0236700 | Msa0277420 | -0.849047 | 4.155675e-60 | -8.615850e-47 |
| Msa0236700 | Msa0288730 | -0.804780 | 1.832513e-49 | -8.615850e-47 |
| Msa0236700 | Msa0289400 | -0.806525 | 7.886480e-50 | -8.615850e-47 |
| Msa0236700 | Msa0292170 | -0.830527 | 2.792934e-55 | -8.615850e-47 |
| Msa0236700 | Msa0306210 | 0.810870 | 9.296896e-51 | -8.615850e-47 |
| Msa0236700 | Msa0317270 | -0.815444 | 9.215152e-52 | -8.615850e-47 |
| Msa0236700 | Msa0326520 | -0.812133 | 4.941269e-51 | -8.615850e-47 |
| Msa0236700 | Msa0344690 | 0.816816 | 4.550579e-52 | -8.615850e-47 |
| Msa0236700 | Msa0356890 | 0.803381 | 3.581847e-49 | -8.615850e-47 |
| Msa0236700 | Msa0365490 | -0.804680 | 1.923230e-49 | -8.615850e-47 |
| Msa0236700 | Msa0381600 | 0.814685 | 1.358581e-51 | -8.615850e-47 |
| Msa0236700 | Msa0388030 | 0.829429 | 5.172899e-55 | -8.615850e-47 |
| Msa0236700 | Msa0392040 | 0.820539 | 6.503502e-53 | -8.615850e-47 |
| Msa0236700 | Msa0394690 | -0.802634 | 5.110968e-49 | -8.615850e-47 |
| Msa0236700 | Msa0410300 | -0.807927 | 3.978788e-50 | -8.615850e-47 |
| Msa0236700 | Msa0420590 | 0.800539 | 1.374796e-48 | -8.615850e-47 |
| Msa0236700 | Msa0420650 | 0.801759 | 7.737776e-49 | -8.615850e-47 |
| Msa0236700 | Msa0422180 | -0.804613 | 1.986162e-49 | -8.615850e-47 |
| Msa0236700 | Msa0433410 | 0.816184 | 6.304400e-52 | -8.615850e-47 |
| Msa0236700 | Msa0436370 | -0.826776 | 2.253680e-54 | -8.615850e-47 |
| Msa0236700 | Msa0437400 | -0.801314 | 9.544499e-49 | -8.615850e-47 |
| Msa0236700 | Msa0457510 | -0.801843 | 7.435189e-49 | -8.615850e-47 |
| Msa0236700 | Msa0458440 | -0.800563 | 1.358875e-48 | -8.615850e-47 |
| Msa0236700 | Msa0461590 | -0.808940 | 2.418751e-50 | -8.615850e-47 |
| Msa0236700 | Msa0464900 | -0.814280 | 1.669217e-51 | -8.615850e-47 |
| Msa0236700 | Msa0469940 | 0.824303 | 8.682567e-54 | -8.615850e-47 |
| Msa0236700 | Msa0473240 | -0.800513 | 1.391077e-48 | -8.615850e-47 |
| Msa0236700 | Msa0484780 | -0.811509 | 6.757703e-51 | -8.615850e-47 |
| Msa0236700 | Msa0506790 | -0.809524 | 1.813267e-50 | -8.615850e-47 |
| Msa0236700 | Msa0517090 | -0.836531 | 8.863179e-57 | -8.615850e-47 |
| Msa0236700 | Msa0517490 | -0.812618 | 3.871785e-51 | -8.615850e-47 |
| Msa0236700 | Msa0526070 | 0.800952 | 1.132237e-48 | -8.615850e-47 |
| Msa0236700 | Msa0526940 | -0.808511 | 2.987212e-50 | -8.615850e-47 |
| Msa0236700 | Msa0530650 | -0.800472 | 1.418548e-48 | -8.615850e-47 |
| Msa0236700 | Msa0532930 | -0.828458 | 8.888827e-55 | -8.615850e-47 |
| Msa0236700 | Msa0554380 | 0.820574 | 6.383378e-53 | -8.615850e-47 |
| Msa0236700 | Msa0559500 | -0.811563 | 6.577094e-51 | -8.615850e-47 |
| Msa0236700 | Msa0572480 | -0.815122 | 1.086847e-51 | -8.615850e-47 |
| Msa0236700 | Msa0573040 | -0.810923 | 9.054955e-51 | -8.615850e-47 |
| Msa0236700 | Msa0579660 | -0.802136 | 6.474220e-49 | -8.615850e-47 |
| Msa0236700 | Msa0587190 | -0.807398 | 5.154839e-50 | -8.615850e-47 |
| Msa0236700 | Msa0598160 | -0.802617 | 5.153951e-49 | -8.615850e-47 |
| Msa0236700 | Msa0613460 | -0.806957 | 6.392342e-50 | -8.615850e-47 |
| Msa0236700 | Msa0617410 | 0.802913 | 4.477546e-49 | -8.615850e-47 |
| Msa0236700 | Msa0624960 | -0.801711 | 7.916973e-49 | -8.615850e-47 |
| Msa0236700 | Msa0626220 | -0.823772 | 1.157160e-53 | -8.615850e-47 |
| Msa0236700 | Msa0626990 | -0.801007 | 1.102884e-48 | -8.615850e-47 |
| Msa0236700 | Msa0641730 | -0.824872 | 6.378135e-54 | -8.615850e-47 |
| Msa0236700 | Msa0648240 | 0.802823 | 4.672089e-49 | -8.615850e-47 |
| Msa0236700 | Msa0654010 | -0.812549 | 4.010097e-51 | -8.615850e-47 |
| Msa0236700 | Msa0654930 | 0.806765 | 7.016079e-50 | -8.615850e-47 |
| Msa0236700 | Msa0677080 | -0.805983 | 1.025567e-49 | -8.615850e-47 |
| Msa0236700 | Msa0679550 | -0.810727 | 9.981619e-51 | -8.615850e-47 |
| Msa0236700 | Msa0689420 | -0.803762 | 2.986507e-49 | -8.615850e-47 |
| Msa0236700 | Msa0691050 | -0.815777 | 7.770519e-52 | -8.615850e-47 |
| Msa0236700 | Msa0715410 | -0.821208 | 4.563123e-53 | -8.615850e-47 |
| Msa0236700 | Msa0721280 | -0.804789 | 1.825144e-49 | -8.615850e-47 |
| Msa0236700 | Msa0735160 | -0.802881 | 4.545092e-49 | -8.615850e-47 |
| Msa0236700 | Msa0749440 | -0.812466 | 4.181044e-51 | -8.615850e-47 |
| Msa0236700 | Msa0750240 | -0.801142 | 1.034991e-48 | -8.615850e-47 |
| Msa0236700 | Msa0751850 | -0.816657 | 4.939163e-52 | -8.615850e-47 |
| Msa0236700 | Msa0756630 | 0.807552 | 4.779699e-50 | -8.615850e-47 |
| Msa0236700 | Msa0784380 | -0.800092 | 1.695056e-48 | -8.615850e-47 |
| Msa0236700 | Msa0793140 | 0.802065 | 6.694899e-49 | -8.615850e-47 |
| Msa0236700 | Msa0799060 | -0.800865 | 1.179250e-48 | -8.615850e-47 |
| Msa0236700 | Msa0831150 | -0.818511 | 1.886870e-52 | -8.615850e-47 |
| Msa0236700 | Msa0832090 | -0.800036 | 1.740139e-48 | -8.615850e-47 |
| Msa0236700 | Msa0861830 | -0.802220 | 6.221253e-49 | -8.615850e-47 |
| Msa0236700 | Msa0864470 | 0.800963 | 1.126082e-48 | -8.615850e-47 |
| Msa0236700 | Msa0866400 | -0.831027 | 2.106948e-55 | -8.615850e-47 |
| Msa0236700 | Msa0866410 | -0.818377 | 2.023052e-52 | -8.615850e-47 |
| Msa0236700 | Msa0873860 | -0.808569 | 2.904182e-50 | -8.615850e-47 |
| Msa0236700 | Msa0892570 | -0.824417 | 8.164512e-54 | -8.615850e-47 |
| Msa0236700 | Msa0924310 | -0.811749 | 5.991740e-51 | -8.615850e-47 |
| Msa0236700 | Msa0942550 | 0.817481 | 3.225261e-52 | -8.615850e-47 |
| Msa0236700 | Msa0960740 | -0.825662 | 4.147824e-54 | -8.615850e-47 |
| Msa0236700 | Msa1001390 | -0.802027 | 6.817274e-49 | -8.615850e-47 |
| Msa0236700 | Msa1006850 | -0.802265 | 6.089335e-49 | -8.615850e-47 |
| Msa0236700 | Msa1008750 | -0.829844 | 4.101436e-55 | -8.615850e-47 |
| Msa0236700 | Msa1012590 | 0.818774 | 1.644440e-52 | -8.615850e-47 |
| Msa0236700 | Msa1021510 | -0.839455 | 1.568973e-57 | -8.615850e-47 |
| Msa0236700 | Msa1029080 | 0.806268 | 8.934086e-50 | -8.615850e-47 |
| Msa0236700 | Msa1031770 | -0.830889 | 2.277343e-55 | -8.615850e-47 |
| Msa0236700 | Msa1031820 | -0.811280 | 7.576503e-51 | -8.615850e-47 |
| Msa0236700 | Msa1032170 | -0.813295 | 2.752097e-51 | -8.615850e-47 |
| Msa0236700 | Msa1037650 | 0.841350 | 5.012140e-58 | -8.615850e-47 |
| Msa0236700 | Msa1039710 | 0.811535 | 6.669779e-51 | -8.615850e-47 |
| Msa0236700 | Msa1040520 | -0.822625 | 2.143270e-53 | -8.615850e-47 |
| Msa0236700 | Msa1050740 | -0.811654 | 6.283866e-51 | -8.615850e-47 |
| Msa0236700 | Msa1054150 | 0.804599 | 1.999425e-49 | -8.615850e-47 |
| Msa0236700 | Msa1070840 | -0.845948 | 2.952583e-59 | -8.615850e-47 |
| Msa0236700 | Msa1073070 | 0.800204 | 1.608641e-48 | -8.615850e-47 |
| Msa0236700 | Msa1075220 | 0.815618 | 8.429696e-52 | -8.615850e-47 |
| Msa0236700 | Msa1076810 | 0.815046 | 1.129652e-51 | -8.615850e-47 |
| Msa0236700 | Msa1079430 | -0.833119 | 6.402398e-56 | -8.615850e-47 |
| Msa0236700 | Msa1080160 | -0.800452 | 1.432014e-48 | -8.615850e-47 |
| Msa0236700 | Msa1087510 | 0.807867 | 4.097391e-50 | -8.615850e-47 |
| Msa0236700 | Msa1098270 | -0.803358 | 3.622121e-49 | -8.615850e-47 |
| Msa0236700 | Msa1105500 | -0.844898 | 5.683941e-59 | -8.615850e-47 |
| Msa0236700 | Msa1118760 | -0.854912 | 8.973211e-62 | -8.615850e-47 |
| Msa0236700 | Msa1127770 | -0.850920 | 1.242360e-60 | -8.615850e-47 |
| Msa0236700 | Msa1130270 | 0.804265 | 2.346771e-49 | -8.615850e-47 |
| Msa0236700 | Msa1133570 | -0.803841 | 2.875700e-49 | -8.615850e-47 |
| Msa0236700 | Msa1139000 | 0.800646 | 1.307408e-48 | -8.615850e-47 |
| Msa0236700 | Msa1144720 | 0.830410 | 2.982803e-55 | -8.615850e-47 |
| Msa0236700 | Msa1152950 | -0.803648 | 3.153179e-49 | -8.615850e-47 |
| Msa0236700 | Msa1156170 | 0.804042 | 2.611323e-49 | -8.615850e-47 |
| Msa0236700 | Msa1158660 | -0.840554 | 8.108277e-58 | -8.615850e-47 |
| Msa0236700 | Msa1162550 | 0.804138 | 2.494302e-49 | -8.615850e-47 |
| Msa0236700 | Msa1163650 | 0.805666 | 1.195786e-49 | -8.615850e-47 |
| Msa0236700 | Msa1168550 | -0.800164 | 1.638329e-48 | -8.615850e-47 |
| Msa0236700 | Msa1168590 | -0.808826 | 2.559300e-50 | -8.615850e-47 |
| Msa0236700 | Msa1174310 | -0.805741 | 1.152885e-49 | -8.615850e-47 |
| Msa0236700 | Msa1177780 | -0.817469 | 3.244280e-52 | -8.615850e-47 |
| Msa0236700 | Msa1204100 | 0.827452 | 1.552500e-54 | -8.615850e-47 |
| Msa0236700 | Msa1207470 | 0.806469 | 8.101644e-50 | -8.615850e-47 |
| Msa0236700 | Msa1211050 | 0.800008 | 1.762593e-48 | -8.615850e-47 |
| Msa0236700 | Msa1257800 | 0.807734 | 4.374065e-50 | -8.615850e-47 |
| Msa0236700 | Msa1262990 | 0.804832 | 1.787884e-49 | -8.615850e-47 |
| Msa0236700 | Msa1263290 | 0.812626 | 3.857574e-51 | -8.615850e-47 |
| Msa0236700 | Msa1270660 | -0.808209 | 3.464799e-50 | -8.615850e-47 |
| Msa0236700 | Msa1286550 | -0.825418 | 4.739240e-54 | -8.615850e-47 |
| Msa0236700 | Msa1296460 | 0.820585 | 6.345234e-53 | -8.615850e-47 |
| Msa0236700 | Msa1307550 | -0.801662 | 8.101527e-49 | -8.615850e-47 |
| Msa0236700 | Msa1314770 | -0.803139 | 4.019478e-49 | -8.615850e-47 |
| Msa0236700 | Msa1319820 | -0.804408 | 2.191210e-49 | -8.615850e-47 |
| Msa0236700 | Msa1329050 | 0.823189 | 1.583930e-53 | -8.615850e-47 |
| Msa0236700 | Msa1335710 | -0.812492 | 4.125766e-51 | -8.615850e-47 |
| Msa0236700 | Msa1340040 | 0.800751 | 1.244021e-48 | -8.615850e-47 |
| Msa0236700 | Msa1352540 | 0.815952 | 7.102316e-52 | -8.615850e-47 |
| Msa0236700 | Msa1357890 | -0.803165 | 3.970020e-49 | -8.615850e-47 |
| Msa0236700 | Msa1359920 | -0.811587 | 6.499622e-51 | -8.615850e-47 |
| Msa0236700 | Msa1371940 | -0.811875 | 5.625198e-51 | -8.615850e-47 |
| Msa0236700 | Msa1372150 | -0.846784 | 1.747537e-59 | -8.615850e-47 |
| Msa0236700 | Msa1376130 | -0.807725 | 4.392972e-50 | -8.615850e-47 |
| Msa0236700 | Msa1376160 | 0.806054 | 9.911223e-50 | -8.615850e-47 |
| Msa0236700 | Msa1376890 | -0.808377 | 3.190639e-50 | -8.615850e-47 |
| Msa0236700 | Msa1377570 | -0.814891 | 1.222678e-51 | -8.615850e-47 |
| Msa0236700 | Msa1379590 | -0.833062 | 6.618499e-56 | -8.615850e-47 |
| Msa0236700 | Msa1379850 | -0.828247 | 9.998483e-55 | -8.615850e-47 |
| Msa0236700 | Msa1392700 | -0.818167 | 2.258138e-52 | -8.615850e-47 |
| Msa0236700 | Msa1394310 | -0.806790 | 6.933630e-50 | -8.615850e-47 |
| Msa0236700 | Msa1400070 | -0.804702 | 1.902619e-49 | -8.615850e-47 |
| Msa0236700 | Msa1404240 | 0.823632 | 1.247703e-53 | -8.615850e-47 |
| Msa0236700 | Msa1417810 | 0.830072 | 3.608823e-55 | -8.615850e-47 |
| Msa0236700 | Msa1421990 | -0.808268 | 3.366979e-50 | -8.615850e-47 |
| Msa0236700 | Msa1422840 | -0.811431 | 7.024240e-51 | -8.615850e-47 |
| Msa0236700 | Msa1424640 | -0.832234 | 1.061860e-55 | -8.615850e-47 |
| Msa0236700 | Msa1431060 | -0.803110 | 4.075323e-49 | -8.615850e-47 |
| Msa0236700 | Msa1433220 | -0.800117 | 1.675461e-48 | -8.615850e-47 |
| Msa0236700 | Msa1435400 | -0.836108 | 1.135061e-56 | -8.615850e-47 |
| Msa0236700 | Msa1440940 | -0.828216 | 1.016765e-54 | -8.615850e-47 |
| Msa0236700 | Msa1445220 | -0.802971 | 4.355800e-49 | -8.615850e-47 |
| Msa0236700 | Msa1445390 | 0.803227 | 3.854124e-49 | -8.615850e-47 |
| Msa0236700 | Msa1445400 | 0.829832 | 4.127594e-55 | -8.615850e-47 |
| Msa0236700 | Msa1449340 | 0.801677 | 8.042946e-49 | -8.615850e-47 |
| Msa0236700 | Msa1449350 | 0.837024 | 6.634507e-57 | -8.615850e-47 |
| Msa0236700 | Msa1451620 | 0.820338 | 7.229220e-53 | -8.615850e-47 |
| Msa0236700 | Msa1465570 | -0.805191 | 1.504083e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0236700 | MtrunA17_Chr2g0285781 | 95.575 | 113 | 5 | 0 | 1 | 113 | 1 | 113 | 3.00e-76 | 220 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0236700 | AT4G18372.1 | 72.321 | 112 | 31 | 0 | 1 | 112 | 1 | 112 | 1.31e-57 | 174 |
| Msa0236700 | AT4G18372.2 | 72.321 | 112 | 31 | 0 | 1 | 112 | 1 | 112 | 1.31e-57 | 174 |
Find 28 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAAGGGAATATTATTCTTC+AGG | 0.307866 | 2_2:-9629891 | Msa0236700:CDS |
| CAAAGATGGAAGATTCTTCT+TGG | 0.344436 | 2_2:-9629936 | Msa0236700:CDS |
| CTTTGATCCCAACCAACATT+TGG | 0.349113 | 2_2:+9629954 | None:intergenic |
| AGGAGGAAGCTCAGACAGTT+TGG | 0.365709 | 2_2:-9630017 | Msa0236700:CDS |
| CTTCCTCCTTGAACTGGAAC+AGG | 0.385459 | 2_2:+9630031 | None:intergenic |
| TCTGAGCTTCCTCCTTGAAC+TGG | 0.448060 | 2_2:+9630025 | None:intergenic |
| CTGTTTCGCCAAATGTTGGT+TGG | 0.454179 | 2_2:-9629962 | Msa0236700:CDS |
| TGTTTCGCCAAATGTTGGTT+GGG | 0.476344 | 2_2:-9629961 | Msa0236700:CDS |
| GAACAACCTGTTCCAGTTCA+AGG | 0.477353 | 2_2:-9630037 | Msa0236700:CDS |
| AAAGATGGAAGATTCTTCTT+GGG | 0.482991 | 2_2:-9629935 | Msa0236700:CDS |
| CCAATGCACCTCTGTTCCAT+TGG | 0.504636 | 2_2:+9629827 | None:intergenic |
| CTTGGGTAACTTCTATTGCA+TGG | 0.505520 | 2_2:-9629918 | Msa0236700:CDS |
| ATGTTGGTTGGGATCAAAGA+TGG | 0.511544 | 2_2:-9629950 | Msa0236700:CDS |
| GGAATCAGATCGAGTCAGTC+GGG | 0.518634 | 2_2:-9629996 | Msa0236700:CDS |
| CCAATGGAACAGAGGTGCAT+TGG | 0.527315 | 2_2:-9629827 | Msa0236700:CDS |
| CATGACGTGCGGCAAGAAGA+AGG | 0.531105 | 2_2:+9629791 | None:intergenic |
| CAGATCGAGTCAGTCGGGTA+AGG | 0.543804 | 2_2:-9629991 | Msa0236700:CDS |
| ACGTTCGTCTCCTTCTCCAA+TGG | 0.549517 | 2_2:-9629843 | Msa0236700:CDS |
| CACCTCTGTTCCATTGGAGA+AGG | 0.557642 | 2_2:+9629833 | None:intergenic |
| TTCTATTGCATGGACAAGCA+AGG | 0.561274 | 2_2:-9629908 | Msa0236700:CDS |
| GCTGCTGTTTCGCCAAATGT+TGG | 0.583498 | 2_2:-9629966 | Msa0236700:CDS |
| TTGCCGCACGTCATGTCACG+TGG | 0.590384 | 2_2:-9629783 | Msa0236700:CDS |
| TGGAATCAGATCGAGTCAGT+CGG | 0.591561 | 2_2:-9629997 | Msa0236700:CDS |
| TCTATTGCATGGACAAGCAA+GGG | 0.616552 | 2_2:-9629907 | Msa0236700:CDS |
| TATTATTCTTCAGGATACTG+TGG | 0.619228 | 2_2:-9629882 | Msa0236700:CDS |
| CTCCTTCTCCAATGGAACAG+AGG | 0.633744 | 2_2:-9629835 | Msa0236700:CDS |
| AATCCACGTGACATGACGTG+CGG | 0.739516 | 2_2:+9629780 | None:intergenic |
| CAACCTGTTCCAGTTCAAGG+AGG | 0.743459 | 2_2:-9630034 | Msa0236700:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGATGGAAGATTCTTCTT+GGG | - | chr2_2:9629850-9629869 | Msa0236700:CDS | 30.0% | |
| TATTATTCTTCAGGATACTG+TGG | - | chr2_2:9629903-9629922 | Msa0236700:CDS | 30.0% | |
| CAAAGATGGAAGATTCTTCT+TGG | - | chr2_2:9629849-9629868 | Msa0236700:CDS | 35.0% | |
| GCAAGGGAATATTATTCTTC+AGG | - | chr2_2:9629894-9629913 | Msa0236700:CDS | 35.0% | |
| ! | TGTTTCGCCAAATGTTGGTT+GGG | - | chr2_2:9629824-9629843 | Msa0236700:CDS | 40.0% |
| CTTTGATCCCAACCAACATT+TGG | + | chr2_2:9629834-9629853 | None:intergenic | 40.0% | |
| ! | ATGTTGGTTGGGATCAAAGA+TGG | - | chr2_2:9629835-9629854 | Msa0236700:CDS | 40.0% |
| CTTGGGTAACTTCTATTGCA+TGG | - | chr2_2:9629867-9629886 | Msa0236700:CDS | 40.0% | |
| TTCTATTGCATGGACAAGCA+AGG | - | chr2_2:9629877-9629896 | Msa0236700:CDS | 40.0% | |
| TCTATTGCATGGACAAGCAA+GGG | - | chr2_2:9629878-9629897 | Msa0236700:CDS | 40.0% | |
| GAACAACCTGTTCCAGTTCA+AGG | - | chr2_2:9629748-9629767 | Msa0236700:CDS | 45.0% | |
| TGGAATCAGATCGAGTCAGT+CGG | - | chr2_2:9629788-9629807 | Msa0236700:CDS | 45.0% | |
| CTGTTTCGCCAAATGTTGGT+TGG | - | chr2_2:9629823-9629842 | Msa0236700:CDS | 45.0% | |
| CAACCTGTTCCAGTTCAAGG+AGG | - | chr2_2:9629751-9629770 | Msa0236700:CDS | 50.0% | |
| CTTCCTCCTTGAACTGGAAC+AGG | + | chr2_2:9629757-9629776 | None:intergenic | 50.0% | |
| TCTGAGCTTCCTCCTTGAAC+TGG | + | chr2_2:9629763-9629782 | None:intergenic | 50.0% | |
| AGGAGGAAGCTCAGACAGTT+TGG | - | chr2_2:9629768-9629787 | Msa0236700:CDS | 50.0% | |
| GGAATCAGATCGAGTCAGTC+GGG | - | chr2_2:9629789-9629808 | Msa0236700:CDS | 50.0% | |
| !! | GCTGCTGTTTCGCCAAATGT+TGG | - | chr2_2:9629819-9629838 | Msa0236700:CDS | 50.0% |
| ACGTTCGTCTCCTTCTCCAA+TGG | - | chr2_2:9629942-9629961 | Msa0236700:CDS | 50.0% | |
| CTCCTTCTCCAATGGAACAG+AGG | - | chr2_2:9629950-9629969 | Msa0236700:CDS | 50.0% | |
| CACCTCTGTTCCATTGGAGA+AGG | + | chr2_2:9629955-9629974 | None:intergenic | 50.0% | |
| CCAATGCACCTCTGTTCCAT+TGG | + | chr2_2:9629961-9629980 | None:intergenic | 50.0% | |
| ! | CCAATGGAACAGAGGTGCAT+TGG | - | chr2_2:9629958-9629977 | Msa0236700:CDS | 50.0% |
| AATCCACGTGACATGACGTG+CGG | + | chr2_2:9630008-9630027 | None:intergenic | 50.0% | |
| CAGATCGAGTCAGTCGGGTA+AGG | - | chr2_2:9629794-9629813 | Msa0236700:CDS | 55.0% | |
| CATGACGTGCGGCAAGAAGA+AGG | + | chr2_2:9629997-9630016 | None:intergenic | 55.0% | |
| TTGCCGCACGTCATGTCACG+TGG | - | chr2_2:9630002-9630021 | Msa0236700:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_2 | gene | 9629733 | 9630074 | 9629733 | ID=Msa0236700;Name=Msa0236700 |
| chr2_2 | mRNA | 9629733 | 9630074 | 9629733 | ID=Msa0236700-mRNA-1;Parent=Msa0236700;Name=Msa0236700-mRNA-1;_AED=0.32;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|113 |
| chr2_2 | exon | 9629733 | 9630074 | 9629733 | ID=Msa0236700-mRNA-1:exon:4208;Parent=Msa0236700-mRNA-1 |
| chr2_2 | CDS | 9629733 | 9630074 | 9629733 | ID=Msa0236700-mRNA-1:cds;Parent=Msa0236700-mRNA-1 |
| Gene Sequence |
| Protein sequence |
