AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0614550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0614550 MtrunA17_Chr4g0050591 98.837 774 9 0 1 774 1 774 0.0 1592
Msa0614550 MtrunA17_Chr2g0278761 85.161 775 114 1 1 774 1 775 0.0 1367
Msa0614550 MtrunA17_Chr1g0194841 57.332 757 311 5 19 772 8 755 0.0 895
Msa0614550 MtrunA17_Chr7g0269871 57.235 774 306 9 8 772 4 761 0.0 894
Msa0614550 MtrunA17_Chr2g0294751 57.778 765 300 9 14 772 7 754 0.0 879
Msa0614550 MtrunA17_Chr1g0207281 55.757 773 302 12 13 764 22 775 0.0 843
Msa0614550 MtrunA17_Chr5g0411571 47.943 778 373 11 20 774 14 782 0.0 753
Msa0614550 MtrunA17_Chr3g0115601 47.247 781 379 15 14 774 9 776 0.0 718
Msa0614550 MtrunA17_Chr5g0425471 46.164 782 391 13 14 774 10 782 0.0 715
Msa0614550 MtrunA17_Chr5g0425431 46.183 786 394 14 10 774 3 780 0.0 714
Msa0614550 MtrunA17_Chr5g0425451 46.164 782 391 13 14 774 10 782 0.0 703
Msa0614550 MtrunA17_Chr5g0425531 45.812 788 398 14 9 774 5 785 0.0 697
Msa0614550 MtrunA17_Chr6g0461191 26.875 640 332 27 54 612 28 612 2.72e-40 157
Msa0614550 MtrunA17_Chr6g0461231 27.328 655 341 29 38 612 13 612 2.73e-40 157
Msa0614550 MtrunA17_Chr3g0118971 27.625 619 354 24 54 612 28 612 5.42e-39 153
Msa0614550 MtrunA17_Chr6g0461211 26.101 636 342 24 54 612 28 612 1.05e-37 149
Msa0614550 MtrunA17_Chr6g0461221 25.693 685 373 29 38 641 14 643 2.73e-37 149
Msa0614550 MtrunA17_Chr3g0104081 25.041 603 354 21 54 593 22 589 2.36e-35 142
Msa0614550 MtrunA17_Chr2g0292341 27.068 665 364 26 54 648 30 643 1.11e-34 140
Msa0614550 MtrunA17_Chr7g0253561 25.875 657 351 28 54 637 4 597 6.89e-34 138
Msa0614550 MtrunA17_Chr5g0429581 26.677 626 354 26 54 612 5 592 1.56e-33 137
Msa0614550 MtrunA17_Chr5g0429541 25.723 622 351 24 54 612 5 578 9.86e-32 131
Msa0614550 MtrunA17_Chr7g0253551 27.009 448 224 18 54 437 29 437 1.24e-27 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0614550 AT5G64570.1 71.483 782 204 4 5 774 10 784 0.0 1189
Msa0614550 AT5G09730.1 68.229 768 232 3 12 774 13 773 0.0 1125
Msa0614550 AT5G64570.2 73.046 627 162 2 154 774 1 626 0.0 995
Msa0614550 AT5G64570.3 73.898 590 141 3 5 588 10 592 0.0 907
Msa0614550 AT3G19620.1 56.275 757 317 7 18 764 6 758 0.0 884
Msa0614550 AT1G02640.1 54.809 759 336 4 15 772 6 758 0.0 868
Msa0614550 AT5G49360.1 57.723 764 305 10 19 772 15 770 0.0 868
Msa0614550 AT5G10560.1 47.656 768 361 9 39 772 30 790 0.0 743
Msa0614550 AT1G78060.1 47.340 752 370 11 40 774 25 767 0.0 723
Msa0614550 AT5G09700.1 66.598 482 155 2 299 774 45 526 0.0 688
Msa0614550 AT3G47050.1 26.942 605 363 23 51 603 7 584 2.75e-39 154
Msa0614550 AT3G47000.1 27.169 611 368 24 54 612 10 595 1.77e-38 152
Msa0614550 AT3G47040.4 24.733 655 374 24 54 643 10 610 1.07e-36 147
Msa0614550 AT3G47010.1 25.926 621 371 23 50 612 7 596 2.07e-36 146
Msa0614550 AT3G47010.2 25.645 620 373 22 50 612 7 595 7.81e-36 144
Msa0614550 AT5G04885.3 26.518 626 359 24 54 616 30 617 2.23e-35 143
Msa0614550 AT5G04885.1 26.518 626 359 24 54 616 30 617 2.23e-35 143
Msa0614550 AT5G04885.2 26.518 626 359 24 54 616 47 634 2.75e-35 143
Msa0614550 AT3G47040.1 24.478 670 382 25 54 643 10 635 2.02e-34 140
Msa0614550 AT3G62710.1 25.731 684 366 28 17 612 11 640 1.13e-33 138
Msa0614550 AT5G20940.1 26.572 636 363 26 41 612 18 613 3.50e-32 133
Msa0614550 AT3G47000.3 29.885 435 235 20 54 437 10 425 2.22e-31 128
Msa0614550 AT5G20950.3 26.034 653 377 28 38 625 90 701 1.05e-30 129
Msa0614550 AT5G20950.2 26.060 637 367 27 54 625 26 623 2.09e-30 127
Msa0614550 AT5G20950.1 26.060 637 367 27 54 625 26 623 2.09e-30 127
Msa0614550 AT3G47040.3 26.786 448 232 18 54 437 10 425 3.69e-30 125
Msa0614550 AT3G47010.4 26.915 457 252 18 50 449 7 438 1.06e-27 117
Msa0614550 AT3G47040.2 23.348 681 384 26 54 643 10 643 1.44e-25 113
Msa0614550 AT3G47050.2 28.713 303 161 11 51 310 7 297 2.14e-23 103
Msa0614550 AT3G47010.3 26.150 413 266 15 215 612 29 417 6.79e-23 102
Msa0614550 AT3G47000.2 26.225 408 273 15 215 612 29 418 2.10e-21 98.2

Find 200 sgRNAs with CRISPR-Local

Find 288 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGACCACCATTCATATTT+TGG 0.140699 4_2:-63614338 None:intergenic
CACCAAACATTAACATATTT+AGG 0.157012 4_2:+63614647 Msa0614550:CDS
ATTAAATCATTGGCAGTTAT+TGG 0.184597 4_2:+63616142 Msa0614550:CDS
CGTGGCAAATGGAAACTAAA+TGG 0.204423 4_2:+63615104 Msa0614550:CDS
TCACTTCTAAAACTTATTTC+TGG 0.205705 4_2:-63617878 None:intergenic
GCTGACCCTGATCTTCTTAA+AGG 0.226486 4_2:+63615074 Msa0614550:CDS
CTATTATATGTAGTTGCTTT+TGG 0.238316 4_2:-63613875 None:intergenic
TGTTCCTACAATCAAGTAAA+TGG 0.245995 4_2:+63615038 Msa0614550:CDS
GCTGAAGTGCAGACATCTTT+TGG 0.254948 4_2:-63616031 None:intergenic
TCTTGACCTCTTCCCCATCT+TGG 0.278328 4_2:-63614673 None:intergenic
TGCAGCTTCCTCAGGGGTCT+TGG 0.289885 4_2:-63615648 None:intergenic
GCCATAGCTGATGTCATCTT+TGG 0.300033 4_2:+63616699 Msa0614550:CDS
TGCCAATGATTTAATAGCTT+TGG 0.304831 4_2:-63616134 None:intergenic
CATGCCTCCTCCAGACATTA+TGG 0.305665 4_2:-63616598 None:intergenic
ATTCGCCAATGCTGGATTCT+TGG 0.315695 4_2:-63614189 None:intergenic
GGGGAAACTGTCTTCTCATT+TGG 0.319056 4_2:+63617339 Msa0614550:CDS
TCAATCACACAGCTCTTAAA+AGG 0.325703 4_2:-63614993 None:intergenic
ATTGTAGACGGCATTGTTAA+TGG 0.328849 4_2:-63615936 None:intergenic
ACCAAACATTAACATATTTA+GGG 0.344637 4_2:+63614648 Msa0614550:CDS
TTAAATCATTGGCAGTTATT+GGG 0.348888 4_2:+63616143 Msa0614550:CDS
AATCCAGCATTGGCGAATTA+CGG 0.359386 4_2:+63614194 Msa0614550:CDS
TCAAATTCTCAAGGTCATAA+TGG 0.369386 4_2:+63614040 Msa0614550:exon
ATCCAGCATTGGCGAATTAC+GGG 0.371963 4_2:+63614195 Msa0614550:CDS
GACTTGGCTGCAGCTTCCTC+AGG 0.373722 4_2:-63615656 None:intergenic
TTTAAGAGCTGTGTGATTGA+TGG 0.379593 4_2:+63614996 Msa0614550:CDS
GAGAATAGGCATAGGAAAAC+TGG 0.380578 4_2:-63614426 None:intergenic
AGTAGCCCGTGATGGGTGTT+AGG 0.382481 4_2:-63613916 None:intergenic
GCTGTCAAGCAAGGGCTTAT+AGG 0.388682 4_2:+63615905 Msa0614550:CDS
ATGCGTCTAGGTTTCTTTGA+TGG 0.391137 4_2:+63615974 Msa0614550:CDS
ATTCTGTGGGTTGGCTATCC+TGG 0.392669 4_2:+63616660 Msa0614550:CDS
GATCCTAGCAAGCAACCTTA+TGG 0.393133 4_2:+63615998 Msa0614550:CDS
CCATAGCTGATGTCATCTTT+GGG 0.402935 4_2:+63616700 Msa0614550:CDS
AGCAGCAACCTTAAGCTTGT+TGG 0.404848 4_2:-63614779 None:intergenic
TGCCAAAGCTATTAAATCAT+TGG 0.406948 4_2:+63616132 Msa0614550:CDS
TTTCCATAAGGTTGCTTGCT+AGG 0.410934 4_2:-63616001 None:intergenic
ATGAGTAATGAGTAGAAAAC+TGG 0.411220 4_2:-63617970 None:intergenic
TGGAGATTCCAACAAGCTTA+AGG 0.418052 4_2:+63614771 Msa0614550:CDS
AATTATATATGTCATATTAG+AGG 0.426795 4_2:-63613969 None:intergenic
ACTAACTGTTGCTGCTGTCC+TGG 0.428145 4_2:-63616534 None:intergenic
TGACAGCACCTTCTGTATAT+CGG 0.431143 4_2:-63615889 None:intergenic
ATTAGTTAAAGCACCACAAT+TGG 0.431784 4_2:+63617395 Msa0614550:CDS
ATAACACCTTTAAGAAGATC+AGG 0.432414 4_2:-63615080 None:intergenic
ACCTTCGTAGTTTCCAATCA+TGG 0.434772 4_2:-63616188 None:intergenic
ATACATTACCACAGCATTGA+AGG 0.443167 4_2:-63614860 None:intergenic
TTGTGGGAACTAAGGCTGTT+AGG 0.443745 4_2:-63616359 None:intergenic
AGGCGAAAACTGCAGATGTT+TGG 0.443791 4_2:-63614157 None:intergenic
GGCCCAATGCTAATGCTACT+AGG 0.444209 4_2:+63616164 Msa0614550:CDS
AACCCGTAATTCGCCAATGC+TGG 0.444375 4_2:-63614197 None:intergenic
GTGATTCTTGCCATAATGTC+TGG 0.445969 4_2:+63616588 Msa0614550:CDS
GCATTGGTGCATTGAACATC+AGG 0.448023 4_2:-63616399 None:intergenic
TGCGCCTCAGAAACACTTGT+TGG 0.449736 4_2:+63617596 Msa0614550:CDS
GTTGGCTATCCTGGAGAAGC+TGG 0.450796 4_2:+63616669 Msa0614550:CDS
GGTTGGCTGGTTTAACATAT+TGG 0.452673 4_2:+63614623 Msa0614550:CDS
CTCATGTTCATATTGGTCAT+TGG 0.453094 4_2:-63617267 None:intergenic
GTTCCCACAAGCTTTGCTGC+TGG 0.455205 4_2:+63616372 Msa0614550:CDS
ATGCACAACGTCGGGTTGGC+TGG 0.455328 4_2:+63614610 Msa0614550:CDS
TCCCTAAATATGTTAATGTT+TGG 0.457701 4_2:-63614649 None:intergenic
CAGCTTCACGAGCTAGCTCC+TGG 0.458313 4_2:-63616057 None:intergenic
CAAGAGCAATGCACAACGTC+GGG 0.459504 4_2:+63614602 Msa0614550:CDS
AGGATTAACATACTTCTTCC+AGG 0.461097 4_2:+63616516 Msa0614550:CDS
GACATTATGGCAAGAATCAC+AGG 0.461878 4_2:-63616585 None:intergenic
ACATATTTAGGGATCCAAGA+TGG 0.461963 4_2:+63614659 Msa0614550:CDS
ATCCGGCCTCATGTTCATAT+TGG 0.462557 4_2:-63617274 None:intergenic
AAAGAAGCTGCAATGAGAAT+AGG 0.464509 4_2:-63614440 None:intergenic
TCCCTTTGTTCTTGATCCTG+AGG 0.465656 4_2:-63617518 None:intergenic
CGGTGCAAATCTTGCAATAG+AGG 0.465750 4_2:+63616479 Msa0614550:CDS
GGGTAGCCTGTAGCAGGATC+CGG 0.467218 4_2:-63617291 None:intergenic
CTTGCTAGCAAATATGCAGC+AGG 0.468402 4_2:+63614718 Msa0614550:CDS
TGTAAGTCACTTGATGTTGT+TGG 0.469882 4_2:+63617462 Msa0614550:CDS
CATATTTAGGGATCCAAGAT+GGG 0.473111 4_2:+63614660 Msa0614550:CDS
CTTCATGGAGTCTCTAACAT+TGG 0.474694 4_2:+63614365 Msa0614550:CDS
GCTGTTGATGTAAGCAGACT+TGG 0.476218 4_2:+63614311 Msa0614550:CDS
GAGAAACCTTACCTTTCCTA+TGG 0.476556 4_2:-63614483 None:intergenic
TGGTGGTCTGAAGCACTTCA+TGG 0.476761 4_2:+63614350 Msa0614550:CDS
AGCAAAGCTTGTGGGAACTA+AGG 0.478113 4_2:-63616367 None:intergenic
ACCTCAGGATCAAGAACAAA+GGG 0.481557 4_2:+63617517 Msa0614550:CDS
CATATTTGCTAGCAAGTAAA+GGG 0.484061 4_2:-63614711 None:intergenic
TTTGGACCAAGGTTTCCATA+AGG 0.489120 4_2:-63616013 None:intergenic
GCATATTTGCTAGCAAGTAA+AGG 0.490578 4_2:-63614712 None:intergenic
TTGAGTGTGGTTGATGAACT+TGG 0.490808 4_2:+63617690 Msa0614550:CDS
AAGGTGTTATCCGTGGCAAA+TGG 0.492172 4_2:+63615093 Msa0614550:CDS
CTTTCCTATGGTTTGAAACA+AGG 0.492728 4_2:-63614471 None:intergenic
GGCATCGTCTAGTGCAGCAT+TGG 0.492888 4_2:-63616415 None:intergenic
CTACCAGGGTAGCCTGTAGC+AGG 0.493617 4_2:-63617297 None:intergenic
ATGAGGCCGGATCCTGCTAC+AGG 0.493861 4_2:+63617285 Msa0614550:CDS
TGAGGCGCATTGTGCACTGC+AGG 0.496274 4_2:-63617582 None:intergenic
AGAAGTTGCAAATGTAGCTA+AGG 0.498193 4_2:+63616560 Msa0614550:CDS
CTTTAAATCATCACGCTCAA+AGG 0.498511 4_2:-63617768 None:intergenic
AATGCTACTAGGGCCATGAT+TGG 0.501422 4_2:+63616175 Msa0614550:CDS
GGTCTACAACAAACTGATGA+TGG 0.502433 4_2:+63614751 Msa0614550:CDS
TTCGAGAAAGTACACTTGAC+AGG 0.502478 4_2:+63617621 Msa0614550:CDS
TAGCCTTTGATATTCACCTC+AGG 0.509602 4_2:+63617502 Msa0614550:CDS
TGTCTGCACTTCAGCAAACC+AGG 0.511708 4_2:+63616039 Msa0614550:CDS
GCTTCCTTGTTTCAAACCAT+AGG 0.512430 4_2:+63614467 Msa0614550:CDS
GTGGCAAATGGAAACTAAAT+GGG 0.513107 4_2:+63615105 Msa0614550:CDS
GCTCGTGAAGCTGCAAGGCA+AGG 0.513237 4_2:+63616067 Msa0614550:CDS
ACTGTCTTCTCATTTGGAGA+TGG 0.513776 4_2:+63617345 Msa0614550:CDS
GCAGCTCCACCAGCTTCTCC+AGG 0.518655 4_2:-63616678 None:intergenic
CTTGTTTCAAACCATAGGAA+AGG 0.519277 4_2:+63614472 Msa0614550:CDS
ATAGGAAAACTGGTTGCTCC+AGG 0.520085 4_2:-63614416 None:intergenic
TAAAATCTGTATGTTCTACC+AGG 0.524873 4_2:-63617312 None:intergenic
CAAACATGATCCTCAGCTAA+AGG 0.526437 4_2:-63617426 None:intergenic
CTCGTGAAGCTGCAAGGCAA+GGG 0.532764 4_2:+63616068 Msa0614550:CDS
GCCCTAGTAGCATTAGCATT+GGG 0.533958 4_2:-63616166 None:intergenic
TTGGGATTCCAAAATATGAA+TGG 0.535603 4_2:+63614330 Msa0614550:CDS
GCAAGTAAAGGGTCTTCTCC+TGG 0.538903 4_2:-63614700 None:intergenic
ATCACAAGCATTCTGTGGGT+TGG 0.539372 4_2:+63616651 Msa0614550:CDS
GGAGTCTCTAACATTGGTCC+AGG 0.541530 4_2:+63614371 Msa0614550:CDS
TGGATTCTTGGCAACATCGC+AGG 0.541711 4_2:-63614177 None:intergenic
GACAGGAAAATCAGAAGCAC+TGG 0.541961 4_2:+63617638 Msa0614550:CDS
CAGCCAGCAGCAAAGCTTGT+GGG 0.542585 4_2:-63616375 None:intergenic
CAGAGGAAAACAGAGACGTT+AGG 0.545414 4_2:-63614083 None:intergenic
GGCTATCCTGGAGAAGCTGG+TGG 0.546561 4_2:+63616672 Msa0614550:CDS
TGTCGCAAAATTATTGTAGA+CGG 0.546588 4_2:-63615948 None:intergenic
GCAGCCAAGTCTATTCTAGC+AGG 0.547377 4_2:+63615668 Msa0614550:CDS
ACTTGGCTGCAGCTTCCTCA+GGG 0.548953 4_2:-63615655 None:intergenic
GGTGTCTGTTCCTTTAGCTG+AGG 0.552719 4_2:+63617416 Msa0614550:CDS
AGCTAGCTCGTGAAGCTGCA+AGG 0.553456 4_2:+63616062 Msa0614550:CDS
GGCCCTAGTAGCATTAGCAT+TGG 0.555454 4_2:-63616167 None:intergenic
TTTACCTGCTAGAATAGACT+TGG 0.558324 4_2:-63615672 None:intergenic
GTGTTAGGAGTGAAAGAAAG+AGG 0.558538 4_2:-63613901 None:intergenic
ACAGAAGGTGCTGTCAAGCA+AGG 0.561917 4_2:+63615896 Msa0614550:CDS
TTAATAGCTTTGGCATTCAG+AGG 0.562815 4_2:-63616124 None:intergenic
TTCACTCCTAACACCCATCA+CGG 0.567877 4_2:+63613910 Msa0614550:five_prime_UTR
AAAAGATCACAAGCATTCTG+TGG 0.568704 4_2:+63616646 Msa0614550:CDS
GATCCTGAGGTGAATATCAA+AGG 0.569135 4_2:-63617505 None:intergenic
AGGGATCCAAGATGGGGAAG+AGG 0.570000 4_2:+63614667 Msa0614550:CDS
AAGGTTGACATTGCAAGAGA+AGG 0.572395 4_2:+63614268 Msa0614550:CDS
AGCAATGCACAACGTCGGGT+TGG 0.573033 4_2:+63614606 Msa0614550:CDS
AAGCAACCTTATGGAAACCT+TGG 0.575163 4_2:+63616007 Msa0614550:CDS
GCCCAATGCTAATGCTACTA+GGG 0.577253 4_2:+63616165 Msa0614550:CDS
TTGACATTGCAAGAGAAGGT+TGG 0.577392 4_2:+63614272 Msa0614550:CDS
CACAGCTCTTAAAAGGTGGT+TGG 0.578720 4_2:-63614986 None:intergenic
CAGAAGGTGCTGTCAAGCAA+GGG 0.579821 4_2:+63615897 Msa0614550:CDS
GGAAAACATATGCTTCATGT+AGG 0.580882 4_2:+63617732 Msa0614550:CDS
TTTGCGACATTAATGCGTCT+AGG 0.581567 4_2:+63615962 Msa0614550:CDS
GGCGCATTGTGCACTGCAGG+AGG 0.582778 4_2:-63617579 None:intergenic
CAGCATATGATGTTGATGAC+TGG 0.584387 4_2:+63614818 Msa0614550:CDS
AGATAGTGAACAACATGGGA+AGG 0.585387 4_2:-63613851 None:intergenic
CACCTCAGGATCAAGAACAA+AGG 0.587124 4_2:+63617516 Msa0614550:CDS
CATTTAGTTTCCATTTGCCA+CGG 0.587656 4_2:-63615103 None:intergenic
TTTCTTGGCCGATATACAGA+AGG 0.588531 4_2:+63615881 Msa0614550:CDS
AAAGATCACAAGCATTCTGT+GGG 0.588555 4_2:+63616647 Msa0614550:CDS
CCCAAAGATGACATCAGCTA+TGG 0.590854 4_2:-63616700 None:intergenic
GACCAATATGAACATGAGGC+CGG 0.590969 4_2:+63617272 Msa0614550:CDS
AAAGCCAACAAGTGTTTCTG+AGG 0.594263 4_2:-63617600 None:intergenic
AGAAATTGAGTGTGGATGCA+AGG 0.597818 4_2:+63614228 Msa0614550:CDS
CTGTTGATGTAAGCAGACTT+GGG 0.597835 4_2:+63614312 Msa0614550:CDS
TGTTGCCAAGAATCCAGCAT+TGG 0.599346 4_2:+63614184 Msa0614550:CDS
TCACTCCTAACACCCATCAC+GGG 0.599970 4_2:+63613911 Msa0614550:five_prime_UTR
TATGCAGCAGGGTACGTGAA+AGG 0.600652 4_2:+63614730 Msa0614550:CDS
TAGAGGCAGAGAGTCATGAC+AGG 0.601562 4_2:+63616496 Msa0614550:CDS
GGGTGAAAGACCTTGTGAGA+AGG 0.601719 4_2:+63614249 Msa0614550:CDS
ATTTGCACCGACTACAATCA+CGG 0.602874 4_2:-63616466 None:intergenic
ACTTTGTCTACATATGATTG+TGG 0.603286 4_2:-63617243 None:intergenic
CATCAACAGCAGAATTCACA+AGG 0.603921 4_2:-63614298 None:intergenic
GGTTGCTCCAGGTATAACAT+TGG 0.604127 4_2:-63614405 None:intergenic
GAAGTTGCAAATGTAGCTAA+GGG 0.606098 4_2:+63616561 Msa0614550:CDS
CTTCTTAAAGGTGTTATCCG+TGG 0.607358 4_2:+63615086 Msa0614550:CDS
ACAGCCAGCAGCAAAGCTTG+TGG 0.607399 4_2:-63616376 None:intergenic
GCGTTACACCTTCAATGCTG+TGG 0.608802 4_2:+63614852 Msa0614550:CDS
CATAATGTCTGGAGGAGGCA+TGG 0.611672 4_2:+63616599 Msa0614550:CDS
CTTGCCATAATGTCTGGAGG+AGG 0.615660 4_2:+63616594 Msa0614550:CDS
AAAGGTGATGTATATTTGCA+TGG 0.623811 4_2:-63616330 None:intergenic
GAAATTGAGTGTGGATGCAA+GGG 0.626342 4_2:+63614229 Msa0614550:CDS
GCAAGAGCAATGCACAACGT+CGG 0.629064 4_2:+63614601 Msa0614550:CDS
GATGCAACCGTGATTGTAGT+CGG 0.629741 4_2:+63616459 Msa0614550:CDS
TGTTTGTAATGATTTGAGTG+TGG 0.631283 4_2:+63617677 Msa0614550:CDS
GGCAACAGAAAAGTTGCCCT+AGG 0.635515 4_2:+63617711 Msa0614550:CDS
TGATTGTGGATACCATGTCA+TGG 0.635666 4_2:-63617229 None:intergenic
GCTGCAATGAGAATAGGCAT+AGG 0.636909 4_2:-63614434 None:intergenic
AAATATACATCACCTTTGCA+AGG 0.638543 4_2:+63616336 Msa0614550:CDS
ATCACACAGCTCTTAAAAGG+TGG 0.639170 4_2:-63614990 None:intergenic
AAAGGAACAGACACCAATTG+TGG 0.639642 4_2:-63617408 None:intergenic
TGGAAGATAGTGAACAACAT+GGG 0.639798 4_2:-63613855 None:intergenic
ACAGGATTGGATTTGAACTG+TGG 0.643755 4_2:+63615854 Msa0614550:intron
GATTGTGGATACCATGTCAT+GGG 0.644060 4_2:-63617228 None:intergenic
AAGGCTGTTAGGCCTTGCAA+AGG 0.647052 4_2:-63616348 None:intergenic
TAACACCTTTAAGAAGATCA+GGG 0.647148 4_2:-63615079 None:intergenic
GGATTCCAAAATATGAATGG+TGG 0.648983 4_2:+63614333 Msa0614550:CDS
GATCCTGCTACAGGCTACCC+TGG 0.649738 4_2:+63617294 Msa0614550:CDS
CAATGACCAATATGAACATG+AGG 0.652360 4_2:+63617268 Msa0614550:CDS
CTTGGCTGCAGCTTCCTCAG+GGG 0.657652 4_2:-63615654 None:intergenic
ACCACAACTCAGCAAAAGTG+TGG 0.657846 4_2:-63614123 None:intergenic
GCAATGTCAACCTTCTCACA+AGG 0.660006 4_2:-63614259 None:intergenic
CAAACAGAACAAAGAAACAG+AGG 0.661188 4_2:-63614100 None:intergenic
AGATCAGGGTCAGCACAAGT+CGG 0.668758 4_2:-63615065 None:intergenic
TTGGAAGATAGTGAACAACA+TGG 0.669298 4_2:-63613856 None:intergenic
AAAATCTGTATGTTCTACCA+GGG 0.669451 4_2:-63617311 None:intergenic
GGAAGAGGTCAAGAAACACC+AGG 0.670289 4_2:+63614682 Msa0614550:CDS
ATATTTAGGGATCCAAGATG+GGG 0.672629 4_2:+63614661 Msa0614550:CDS
GTGGAAGGCTACCCATGACA+TGG 0.674570 4_2:+63617217 Msa0614550:CDS
TATGATGTTGATGACTGGAA+AGG 0.681867 4_2:+63614823 Msa0614550:CDS
CTGCAACAAGAAATTGAGTG+TGG 0.685493 4_2:+63614220 Msa0614550:CDS
GCCATGATTGGAAACTACGA+AGG 0.687224 4_2:+63616187 Msa0614550:CDS
AATCACGGTTGCATCTGCTG+AGG 0.690719 4_2:-63616451 None:intergenic
TTGCTAGCAAATATGCAGCA+GGG 0.692959 4_2:+63614719 Msa0614550:CDS
ATTCTTGCCATAATGTCTGG+AGG 0.713271 4_2:+63616591 Msa0614550:CDS
AACATGGGAAGGAATCTCTG+TGG 0.741639 4_2:-63613840 None:intergenic
ACATTACACCAAGACCCCTG+AGG 0.763610 4_2:+63615640 Msa0614550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTTTTAAATATTGTTTTA+TGG + chr4_2:63614550-63614569 Msa0614550:intron 10.0%
!!! ATATTTTGTTTATATTTGAA+TGG - chr4_2:63615173-63615192 None:intergenic 10.0%
!!! CATATAAAAGATAATTTTTT+TGG - chr4_2:63615339-63615358 None:intergenic 10.0%
!! AATTATATATGTCATATTAG+AGG - chr4_2:63613972-63613991 None:intergenic 15.0%
!! AAAACACAAAAATAAGTAAA+AGG - chr4_2:63614021-63614040 None:intergenic 15.0%
!!! AATTTTTTTGGATGTAAATT+GGG - chr4_2:63615327-63615346 None:intergenic 15.0%
!!! TAATTTTTTTGGATGTAAAT+TGG - chr4_2:63615328-63615347 None:intergenic 15.0%
!!! ATTTTTCCTATAAAAGTTAT+AGG - chr4_2:63616230-63616249 None:intergenic 15.0%
!! AATGATAATTATGTAATATG+AGG - chr4_2:63616765-63616784 None:intergenic 15.0%
!! CATATATTATTGAGAATTAT+TGG - chr4_2:63617085-63617104 None:intergenic 15.0%
!!! CAACTTAATTTTTTTTATTC+AGG + chr4_2:63617195-63617214 Msa0614550:intron 15.0%
!!! ATTTTTTAAGGAAATCAATA+AGG - chr4_2:63617823-63617842 None:intergenic 15.0%
!! TGATTTCCTTAAAAAATTTA+AGG + chr4_2:63617826-63617845 Msa0614550:three_prime_UTR 15.0%
!! GATTTCCTTAAAAAATTTAA+GGG + chr4_2:63617827-63617846 Msa0614550:three_prime_UTR 15.0%
!! ACCAAACATTAACATATTTA+GGG + chr4_2:63614648-63614667 Msa0614550:CDS 20.0%
!!! TAATTGTTGTTGATTTTGTT+AGG + chr4_2:63614935-63614954 Msa0614550:intron 20.0%
!!! TGTACAAATTTTGATTTTAC+AGG + chr4_2:63615836-63615855 Msa0614550:intron 20.0%
!!! AAATTTTGATTTTACAGGAT+TGG + chr4_2:63615841-63615860 Msa0614550:intron 20.0%
!!! CTTTTTATTAGTTTTAGCAA+AGG - chr4_2:63616631-63616650 None:intergenic 20.0%
!! TAATTATCATTCCAATAACT+TGG + chr4_2:63616774-63616793 Msa0614550:intron 20.0%
!!! AACAACTTTGATTTTAAACT+CGG + chr4_2:63616858-63616877 Msa0614550:intron 20.0%
!!! CTTAATTTTTTTTATTCAGG+TGG + chr4_2:63617198-63617217 Msa0614550:intron 20.0%
!!! AGAACATACAGATTTTATAA+AGG + chr4_2:63617318-63617337 Msa0614550:CDS 20.0%
!!! GAACATACAGATTTTATAAA+GGG + chr4_2:63617319-63617338 Msa0614550:CDS 20.0%
!!! AACATACAGATTTTATAAAG+GGG + chr4_2:63617320-63617339 Msa0614550:CDS 20.0%
!!! CAATTTTTTAATGCAAGTTA+TGG - chr4_2:63617855-63617874 None:intergenic 20.0%
!!! AAAAAAAAATCAGTATTGAC+TGG + chr4_2:63617946-63617965 Msa0614550:three_prime_UTR 20.0%
!!! TCTTTTATTTCAGCTTTTCA+CGG + chr4_2:63613808-63613827 Msa0614550:five_prime_UTR 25.0%
!!! CTATTATATGTAGTTGCTTT+TGG - chr4_2:63613878-63613897 None:intergenic 25.0%
!! TTTGTGTTTTCAAATTCTCA+AGG + chr4_2:63614031-63614050 Msa0614550:five_prime_UTR 25.0%
! CACCAAACATTAACATATTT+AGG + chr4_2:63614647-63614666 Msa0614550:CDS 25.0%
! TCCCTAAATATGTTAATGTT+TGG - chr4_2:63614652-63614671 None:intergenic 25.0%
!! TTTTCCATTTACTTGATTGT+AGG - chr4_2:63615045-63615064 None:intergenic 25.0%
! AGTGAGATAAAAGAATTAAG+AGG - chr4_2:63615150-63615169 None:intergenic 25.0%
! AAATCGAAAACAGAAAAGAA+TGG - chr4_2:63615527-63615546 None:intergenic 25.0%
! TTACACAATCACATATTCAT+AGG + chr4_2:63615710-63615729 Msa0614550:intron 25.0%
!!! AGCTTCTTTAGAATTATACA+AGG + chr4_2:63615790-63615809 Msa0614550:intron 25.0%
! ATTAAATCATTGGCAGTTAT+TGG + chr4_2:63616142-63616161 Msa0614550:CDS 25.0%
! TTAAATCATTGGCAGTTATT+GGG + chr4_2:63616143-63616162 Msa0614550:CDS 25.0%
! TCACTAGGATCTAATTATAT+GGG + chr4_2:63617161-63617180 Msa0614550:intron 25.0%
!!! ATTTTTTTTATTCAGGTGGA+AGG + chr4_2:63617202-63617221 Msa0614550:intron 25.0%
!!! TGGTTCCCTTAAATTTTTTA+AGG - chr4_2:63617835-63617854 None:intergenic 25.0%
!!! TCACTTCTAAAACTTATTTC+TGG - chr4_2:63617881-63617900 None:intergenic 25.0%
TCAAATTCTCAAGGTCATAA+TGG + chr4_2:63614040-63614059 Msa0614550:exon 30.0%
TTGGGATTCCAAAATATGAA+TGG + chr4_2:63614330-63614349 Msa0614550:CDS 30.0%
! CATTTTTCCAATGTTATACC+TGG + chr4_2:63614398-63614417 Msa0614550:CDS 30.0%
TATCATATCCTGCATGTTAA+TGG + chr4_2:63614507-63614526 Msa0614550:intron 30.0%
TATTTACACCATTAACATGC+AGG - chr4_2:63614518-63614537 None:intergenic 30.0%
!! GTTTTATGGCTATTTTGTGT+AGG + chr4_2:63614564-63614583 Msa0614550:intron 30.0%
! CATATTTGCTAGCAAGTAAA+GGG - chr4_2:63614714-63614733 None:intergenic 30.0%
!! AGCTAGTTAATGTGTTTTTG+AGG + chr4_2:63614905-63614924 Msa0614550:intron 30.0%
TGTTCCTACAATCAAGTAAA+TGG + chr4_2:63615038-63615057 Msa0614550:CDS 30.0%
! TAACACCTTTAAGAAGATCA+GGG - chr4_2:63615082-63615101 None:intergenic 30.0%
! ATAACACCTTTAAGAAGATC+AGG - chr4_2:63615083-63615102 None:intergenic 30.0%
TTGTTCACTGTAATTCTATC+CGG + chr4_2:63615263-63615282 Msa0614550:intron 30.0%
CAAAATAAACTCATGTTTGC+TGG - chr4_2:63615468-63615487 None:intergenic 30.0%
TGTCGCAAAATTATTGTAGA+CGG - chr4_2:63615951-63615970 None:intergenic 30.0%
TGCCAAAGCTATTAAATCAT+TGG + chr4_2:63616132-63616151 Msa0614550:CDS 30.0%
TGCCAATGATTTAATAGCTT+TGG - chr4_2:63616137-63616156 None:intergenic 30.0%
CCTATAAAAGTTATAGGAGT+GGG - chr4_2:63616224-63616243 None:intergenic 30.0%
TCCTATAAAAGTTATAGGAG+TGG - chr4_2:63616225-63616244 None:intergenic 30.0%
!! AACAAGTTCTAAAACTTTGC+AGG + chr4_2:63616303-63616322 Msa0614550:intron 30.0%
AAAGGTGATGTATATTTGCA+TGG - chr4_2:63616333-63616352 None:intergenic 30.0%
AAATATACATCACCTTTGCA+AGG + chr4_2:63616336-63616355 Msa0614550:CDS 30.0%
CAAAAGGAAATCCAAGTTAT+TGG - chr4_2:63616788-63616807 None:intergenic 30.0%
TGCTTGAAAATTGTGACAAA+AGG - chr4_2:63616804-63616823 None:intergenic 30.0%
!! ATTTCAAGTTTGTAGCAACT+TGG - chr4_2:63616942-63616961 None:intergenic 30.0%
CATATAATTAGATCCTAGTG+AGG - chr4_2:63617162-63617181 None:intergenic 30.0%
CTCACTAGGATCTAATTATA+TGG + chr4_2:63617160-63617179 Msa0614550:intron 30.0%
AGGATCTAATTATATGGGTA+TGG + chr4_2:63617166-63617185 Msa0614550:intron 30.0%
ACTTTGTCTACATATGATTG+TGG - chr4_2:63617246-63617265 None:intergenic 30.0%
AAAATCTGTATGTTCTACCA+GGG - chr4_2:63617314-63617333 None:intergenic 30.0%
TAAAATCTGTATGTTCTACC+AGG - chr4_2:63617315-63617334 None:intergenic 30.0%
! ATTAGTTAAAGCACCACAAT+TGG + chr4_2:63617395-63617414 Msa0614550:CDS 30.0%
! ATCAAGTGACTTACATTTTG+AGG - chr4_2:63617457-63617476 None:intergenic 30.0%
TAGAGAACAAAAACACTGTT+TGG - chr4_2:63617557-63617576 None:intergenic 30.0%
TGTTTGTAATGATTTGAGTG+TGG + chr4_2:63617677-63617696 Msa0614550:CDS 30.0%
! ATGAAGCATATGTTTTCCTA+GGG - chr4_2:63617730-63617749 None:intergenic 30.0%
ATGAGTAATGAGTAGAAAAC+TGG - chr4_2:63617973-63617992 None:intergenic 30.0%
TGGAAGATAGTGAACAACAT+GGG - chr4_2:63613858-63613877 None:intergenic 35.0%
TTGGAAGATAGTGAACAACA+TGG - chr4_2:63613859-63613878 None:intergenic 35.0%
CAAACAGAACAAAGAAACAG+AGG - chr4_2:63614103-63614122 None:intergenic 35.0%
GGATTCCAAAATATGAATGG+TGG + chr4_2:63614333-63614352 Msa0614550:CDS 35.0%
! TCAGACCACCATTCATATTT+TGG - chr4_2:63614341-63614360 None:intergenic 35.0%
ACATTGGAAAAATGAGTTCC+TGG - chr4_2:63614392-63614411 None:intergenic 35.0%
AAAGAAGCTGCAATGAGAAT+AGG - chr4_2:63614443-63614462 None:intergenic 35.0%
! CTTTCCTATGGTTTGAAACA+AGG - chr4_2:63614474-63614493 None:intergenic 35.0%
CTTGTTTCAAACCATAGGAA+AGG + chr4_2:63614472-63614491 Msa0614550:CDS 35.0%
TTCAAACCATAGGAAAGGTA+AGG + chr4_2:63614477-63614496 Msa0614550:intron 35.0%
!! TTATGGCTATTTTGTGTAGG+TGG + chr4_2:63614567-63614586 Msa0614550:intron 35.0%
ACATATTTAGGGATCCAAGA+TGG + chr4_2:63614659-63614678 Msa0614550:CDS 35.0%
CATATTTAGGGATCCAAGAT+GGG + chr4_2:63614660-63614679 Msa0614550:CDS 35.0%
ATATTTAGGGATCCAAGATG+GGG + chr4_2:63614661-63614680 Msa0614550:CDS 35.0%
! GCATATTTGCTAGCAAGTAA+AGG - chr4_2:63614715-63614734 None:intergenic 35.0%
!! TATGATGTTGATGACTGGAA+AGG + chr4_2:63614823-63614842 Msa0614550:CDS 35.0%
! ATACATTACCACAGCATTGA+AGG - chr4_2:63614863-63614882 None:intergenic 35.0%
TCAATCACACAGCTCTTAAA+AGG - chr4_2:63614996-63615015 None:intergenic 35.0%
TTTAAGAGCTGTGTGATTGA+TGG + chr4_2:63614996-63615015 Msa0614550:CDS 35.0%
! CATTTAGTTTCCATTTGCCA+CGG - chr4_2:63615106-63615125 None:intergenic 35.0%
GTGGCAAATGGAAACTAAAT+GGG + chr4_2:63615105-63615124 Msa0614550:CDS 35.0%
TGTTCACTGTAATTCTATCC+GGG + chr4_2:63615264-63615283 Msa0614550:intron 35.0%
CAAAAACTAATTCCTCAACC+CGG - chr4_2:63615285-63615304 None:intergenic 35.0%
TTTACCTGCTAGAATAGACT+TGG - chr4_2:63615675-63615694 None:intergenic 35.0%
! TTGAACTGTGGAAGTTTTCT+TGG + chr4_2:63615866-63615885 Msa0614550:CDS 35.0%
ATTGTAGACGGCATTGTTAA+TGG - chr4_2:63615939-63615958 None:intergenic 35.0%
!! TTAATAGCTTTGGCATTCAG+AGG - chr4_2:63616127-63616146 None:intergenic 35.0%
! CCCACTCCTATAACTTTTAT+AGG + chr4_2:63616221-63616240 Msa0614550:intron 35.0%
AGGATTAACATACTTCTTCC+AGG + chr4_2:63616516-63616535 Msa0614550:CDS 35.0%
AGAAGTTGCAAATGTAGCTA+AGG + chr4_2:63616560-63616579 Msa0614550:CDS 35.0%
GAAGTTGCAAATGTAGCTAA+GGG + chr4_2:63616561-63616580 Msa0614550:CDS 35.0%
AAAAGATCACAAGCATTCTG+TGG + chr4_2:63616646-63616665 Msa0614550:CDS 35.0%
AAAGATCACAAGCATTCTGT+GGG + chr4_2:63616647-63616666 Msa0614550:CDS 35.0%
GTCGTTCAATCTTGATCTAA+TGG + chr4_2:63616914-63616933 Msa0614550:intron 35.0%
TTGCTAAATGCATGAATGTG+TGG + chr4_2:63616996-63617015 Msa0614550:intron 35.0%
TGAATGTGTGGAAATTGTGA+CGG + chr4_2:63617008-63617027 Msa0614550:intron 35.0%
ACGGCTTACAACCTAAATAA+TGG + chr4_2:63617027-63617046 Msa0614550:intron 35.0%
CATGAGCGAAACCATTATTT+AGG - chr4_2:63617041-63617060 None:intergenic 35.0%
AGAATTATTGGAACCTTGTG+GGG - chr4_2:63617073-63617092 None:intergenic 35.0%
GAGAATTATTGGAACCTTGT+GGG - chr4_2:63617074-63617093 None:intergenic 35.0%
TGAGAATTATTGGAACCTTG+TGG - chr4_2:63617075-63617094 None:intergenic 35.0%
CTCATGTTCATATTGGTCAT+TGG - chr4_2:63617270-63617289 None:intergenic 35.0%
CAATGACCAATATGAACATG+AGG + chr4_2:63617268-63617287 Msa0614550:CDS 35.0%
TGTAAGTCACTTGATGTTGT+TGG + chr4_2:63617462-63617481 Msa0614550:CDS 35.0%
! CATGAAGCATATGTTTTCCT+AGG - chr4_2:63617731-63617750 None:intergenic 35.0%
GGAAAACATATGCTTCATGT+AGG + chr4_2:63617732-63617751 Msa0614550:CDS 35.0%
CTTTAAATCATCACGCTCAA+AGG - chr4_2:63617771-63617790 None:intergenic 35.0%
AGATAGTGAACAACATGGGA+AGG - chr4_2:63613854-63613873 None:intergenic 40.0%
GTGTTAGGAGTGAAAGAAAG+AGG - chr4_2:63613904-63613923 None:intergenic 40.0%
!! ATTTTTCAGTAGCCCGTGAT+GGG - chr4_2:63613926-63613945 None:intergenic 40.0%
! AGGTGTTCTGTTTTCAACAG+AGG - chr4_2:63614066-63614085 None:intergenic 40.0%
!! AATCCAGCATTGGCGAATTA+CGG + chr4_2:63614194-63614213 Msa0614550:CDS 40.0%
CTGCAACAAGAAATTGAGTG+TGG + chr4_2:63614220-63614239 Msa0614550:CDS 40.0%
AGAAATTGAGTGTGGATGCA+AGG + chr4_2:63614228-63614247 Msa0614550:CDS 40.0%
GAAATTGAGTGTGGATGCAA+GGG + chr4_2:63614229-63614248 Msa0614550:CDS 40.0%
AAGGTTGACATTGCAAGAGA+AGG + chr4_2:63614268-63614287 Msa0614550:CDS 40.0%
TTGACATTGCAAGAGAAGGT+TGG + chr4_2:63614272-63614291 Msa0614550:CDS 40.0%
CATCAACAGCAGAATTCACA+AGG - chr4_2:63614301-63614320 None:intergenic 40.0%
CTGTTGATGTAAGCAGACTT+GGG + chr4_2:63614312-63614331 Msa0614550:CDS 40.0%
CTTCATGGAGTCTCTAACAT+TGG + chr4_2:63614365-63614384 Msa0614550:CDS 40.0%
GAGAATAGGCATAGGAAAAC+TGG - chr4_2:63614429-63614448 None:intergenic 40.0%
! GCTTCCTTGTTTCAAACCAT+AGG + chr4_2:63614467-63614486 Msa0614550:CDS 40.0%
GAGAAACCTTACCTTTCCTA+TGG - chr4_2:63614486-63614505 None:intergenic 40.0%
! TTTTGTGTAGGTGGTTTCGA+CGG + chr4_2:63614576-63614595 Msa0614550:intron 40.0%
!! GGTTGGCTGGTTTAACATAT+TGG + chr4_2:63614623-63614642 Msa0614550:CDS 40.0%
TTGCTAGCAAATATGCAGCA+GGG + chr4_2:63614719-63614738 Msa0614550:CDS 40.0%
GGTCTACAACAAACTGATGA+TGG + chr4_2:63614751-63614770 Msa0614550:CDS 40.0%
TGGAGATTCCAACAAGCTTA+AGG + chr4_2:63614771-63614790 Msa0614550:CDS 40.0%
!! CAGCATATGATGTTGATGAC+TGG + chr4_2:63614818-63614837 Msa0614550:CDS 40.0%
ATCACACAGCTCTTAAAAGG+TGG - chr4_2:63614993-63615012 None:intergenic 40.0%
!! CTTCTTAAAGGTGTTATCCG+TGG + chr4_2:63615086-63615105 Msa0614550:CDS 40.0%
CGTGGCAAATGGAAACTAAA+TGG + chr4_2:63615104-63615123 Msa0614550:CDS 40.0%
ACAGGATTGGATTTGAACTG+TGG + chr4_2:63615854-63615873 Msa0614550:intron 40.0%
TTTCTTGGCCGATATACAGA+AGG + chr4_2:63615881-63615900 Msa0614550:CDS 40.0%
TGACAGCACCTTCTGTATAT+CGG - chr4_2:63615892-63615911 None:intergenic 40.0%
TTTGCGACATTAATGCGTCT+AGG + chr4_2:63615962-63615981 Msa0614550:CDS 40.0%
ATGCGTCTAGGTTTCTTTGA+TGG + chr4_2:63615974-63615993 Msa0614550:CDS 40.0%
TTTCCATAAGGTTGCTTGCT+AGG - chr4_2:63616004-63616023 None:intergenic 40.0%
AAGCAACCTTATGGAAACCT+TGG + chr4_2:63616007-63616026 Msa0614550:CDS 40.0%
TTTGGACCAAGGTTTCCATA+AGG - chr4_2:63616016-63616035 None:intergenic 40.0%
!! GTGTTGCTTAAAAACTGTGC+AGG + chr4_2:63616094-63616113 Msa0614550:CDS 40.0%
ACCTTCGTAGTTTCCAATCA+TGG - chr4_2:63616191-63616210 None:intergenic 40.0%
!! ATTTGCACCGACTACAATCA+CGG - chr4_2:63616469-63616488 None:intergenic 40.0%
GACATTATGGCAAGAATCAC+AGG - chr4_2:63616588-63616607 None:intergenic 40.0%
GTGATTCTTGCCATAATGTC+TGG + chr4_2:63616588-63616607 Msa0614550:CDS 40.0%
ATTCTTGCCATAATGTCTGG+AGG + chr4_2:63616591-63616610 Msa0614550:CDS 40.0%
CCATAGCTGATGTCATCTTT+GGG + chr4_2:63616700-63616719 Msa0614550:CDS 40.0%
! GTCAGTGAGATTGACTTACT+TGG - chr4_2:63616735-63616754 None:intergenic 40.0%
ATGCAGTAAGACGAAGTTGT+TGG + chr4_2:63616831-63616850 Msa0614550:intron 40.0%
GCCATTAGATCAAGATCGAA+CGG - chr4_2:63616970-63616989 None:intergenic 40.0%
GATCTTGATCTAATGGCTTC+AGG + chr4_2:63616973-63616992 Msa0614550:intron 40.0%
AATTATTGGAACCTTGTGGG+GGG - chr4_2:63617071-63617090 None:intergenic 40.0%
GAATTATTGGAACCTTGTGG+GGG - chr4_2:63617072-63617091 None:intergenic 40.0%
GATTGTGGATACCATGTCAT+GGG - chr4_2:63617231-63617250 None:intergenic 40.0%
TGATTGTGGATACCATGTCA+TGG - chr4_2:63617232-63617251 None:intergenic 40.0%
ACTGTCTTCTCATTTGGAGA+TGG + chr4_2:63617345-63617364 Msa0614550:CDS 40.0%
AAAGGAACAGACACCAATTG+TGG - chr4_2:63617411-63617430 None:intergenic 40.0%
CAAACATGATCCTCAGCTAA+AGG - chr4_2:63617429-63617448 None:intergenic 40.0%
TAGCCTTTGATATTCACCTC+AGG + chr4_2:63617502-63617521 Msa0614550:CDS 40.0%
GATCCTGAGGTGAATATCAA+AGG - chr4_2:63617508-63617527 None:intergenic 40.0%
ACCTCAGGATCAAGAACAAA+GGG + chr4_2:63617517-63617536 Msa0614550:CDS 40.0%
! AAAGCCAACAAGTGTTTCTG+AGG - chr4_2:63617603-63617622 None:intergenic 40.0%
! TTCGAGAAAGTACACTTGAC+AGG + chr4_2:63617621-63617640 Msa0614550:CDS 40.0%
!! TTGAGTGTGGTTGATGAACT+TGG + chr4_2:63617690-63617709 Msa0614550:CDS 40.0%
AACATGGGAAGGAATCTCTG+TGG - chr4_2:63613843-63613862 None:intergenic 45.0%
TTCACTCCTAACACCCATCA+CGG + chr4_2:63613910-63613929 Msa0614550:five_prime_UTR 45.0%
!! CATTTTTCAGTAGCCCGTGA+TGG - chr4_2:63613927-63613946 None:intergenic 45.0%
CAGAGGAAAACAGAGACGTT+AGG - chr4_2:63614086-63614105 None:intergenic 45.0%
ACCACAACTCAGCAAAAGTG+TGG - chr4_2:63614126-63614145 None:intergenic 45.0%
AGGCGAAAACTGCAGATGTT+TGG - chr4_2:63614160-63614179 None:intergenic 45.0%
TGTTGCCAAGAATCCAGCAT+TGG + chr4_2:63614184-63614203 Msa0614550:CDS 45.0%
ATTCGCCAATGCTGGATTCT+TGG - chr4_2:63614192-63614211 None:intergenic 45.0%
!! ATCCAGCATTGGCGAATTAC+GGG + chr4_2:63614195-63614214 Msa0614550:CDS 45.0%
GCAATGTCAACCTTCTCACA+AGG - chr4_2:63614262-63614281 None:intergenic 45.0%
GCTGTTGATGTAAGCAGACT+TGG + chr4_2:63614311-63614330 Msa0614550:CDS 45.0%
GGTTGCTCCAGGTATAACAT+TGG - chr4_2:63614408-63614427 None:intergenic 45.0%
ATAGGAAAACTGGTTGCTCC+AGG - chr4_2:63614419-63614438 None:intergenic 45.0%
GCTGCAATGAGAATAGGCAT+AGG - chr4_2:63614437-63614456 None:intergenic 45.0%
CTTGCTAGCAAATATGCAGC+AGG + chr4_2:63614718-63614737 Msa0614550:CDS 45.0%
AGCAGCAACCTTAAGCTTGT+TGG - chr4_2:63614782-63614801 None:intergenic 45.0%
CACAGCTCTTAAAAGGTGGT+TGG - chr4_2:63614989-63615008 None:intergenic 45.0%
GCTGACCCTGATCTTCTTAA+AGG + chr4_2:63615074-63615093 Msa0614550:CDS 45.0%
! AAGGTGTTATCCGTGGCAAA+TGG + chr4_2:63615093-63615112 Msa0614550:CDS 45.0%
CTGTAATTCTATCCGGGTTG+AGG + chr4_2:63615270-63615289 Msa0614550:intron 45.0%
GATCCTAGCAAGCAACCTTA+TGG + chr4_2:63615998-63616017 Msa0614550:CDS 45.0%
! TGCAGACATCTTTTGGACCA+AGG - chr4_2:63616027-63616046 None:intergenic 45.0%
! GCTGAAGTGCAGACATCTTT+TGG - chr4_2:63616034-63616053 None:intergenic 45.0%
GCCCAATGCTAATGCTACTA+GGG + chr4_2:63616165-63616184 Msa0614550:CDS 45.0%
GCCCTAGTAGCATTAGCATT+GGG - chr4_2:63616169-63616188 None:intergenic 45.0%
AATGCTACTAGGGCCATGAT+TGG + chr4_2:63616175-63616194 Msa0614550:CDS 45.0%
GCCATGATTGGAAACTACGA+AGG + chr4_2:63616187-63616206 Msa0614550:CDS 45.0%
TTGTGGGAACTAAGGCTGTT+AGG - chr4_2:63616362-63616381 None:intergenic 45.0%
AGCAAAGCTTGTGGGAACTA+AGG - chr4_2:63616370-63616389 None:intergenic 45.0%
! GCATTGGTGCATTGAACATC+AGG - chr4_2:63616402-63616421 None:intergenic 45.0%
! TGCTGAGGCTGCAATCTTTT+TGG - chr4_2:63616439-63616458 None:intergenic 45.0%
GATGCAACCGTGATTGTAGT+CGG + chr4_2:63616459-63616478 Msa0614550:CDS 45.0%
CGGTGCAAATCTTGCAATAG+AGG + chr4_2:63616479-63616498 Msa0614550:CDS 45.0%
ATCACAAGCATTCTGTGGGT+TGG + chr4_2:63616651-63616670 Msa0614550:CDS 45.0%
GCCATAGCTGATGTCATCTT+TGG + chr4_2:63616699-63616718 Msa0614550:CDS 45.0%
CCCAAAGATGACATCAGCTA+TGG - chr4_2:63616703-63616722 None:intergenic 45.0%
GCCGTTCGATCTTGATCTAA+TGG + chr4_2:63616966-63616985 Msa0614550:intron 45.0%
ATATGCTCAGTGACCTCACT+AGG + chr4_2:63617146-63617165 Msa0614550:intron 45.0%
GACCAATATGAACATGAGGC+CGG + chr4_2:63617272-63617291 Msa0614550:CDS 45.0%
ATCCGGCCTCATGTTCATAT+TGG - chr4_2:63617277-63617296 None:intergenic 45.0%
GGGGAAACTGTCTTCTCATT+TGG + chr4_2:63617339-63617358 Msa0614550:CDS 45.0%
CACCTCAGGATCAAGAACAA+AGG + chr4_2:63617516-63617535 Msa0614550:CDS 45.0%
TCCCTTTGTTCTTGATCCTG+AGG - chr4_2:63617521-63617540 None:intergenic 45.0%
! GACAGGAAAATCAGAAGCAC+TGG + chr4_2:63617638-63617657 Msa0614550:CDS 45.0%
TCACTCCTAACACCCATCAC+GGG + chr4_2:63613911-63613930 Msa0614550:five_prime_UTR 50.0%
! GCCACACTTTTGCTGAGTTG+TGG + chr4_2:63614122-63614141 Msa0614550:CDS 50.0%
TGGATTCTTGGCAACATCGC+AGG - chr4_2:63614180-63614199 None:intergenic 50.0%
AACCCGTAATTCGCCAATGC+TGG - chr4_2:63614200-63614219 None:intergenic 50.0%
GGGTGAAAGACCTTGTGAGA+AGG + chr4_2:63614249-63614268 Msa0614550:CDS 50.0%
! TGGTGGTCTGAAGCACTTCA+TGG + chr4_2:63614350-63614369 Msa0614550:CDS 50.0%
GGAGTCTCTAACATTGGTCC+AGG + chr4_2:63614371-63614390 Msa0614550:CDS 50.0%
GCAAGAGCAATGCACAACGT+CGG + chr4_2:63614601-63614620 Msa0614550:CDS 50.0%
CAAGAGCAATGCACAACGTC+GGG + chr4_2:63614602-63614621 Msa0614550:CDS 50.0%
TCTTGACCTCTTCCCCATCT+TGG - chr4_2:63614676-63614695 None:intergenic 50.0%
GGAAGAGGTCAAGAAACACC+AGG + chr4_2:63614682-63614701 Msa0614550:CDS 50.0%
GCAAGTAAAGGGTCTTCTCC+TGG - chr4_2:63614703-63614722 None:intergenic 50.0%
TATGCAGCAGGGTACGTGAA+AGG + chr4_2:63614730-63614749 Msa0614550:CDS 50.0%
GCGTTACACCTTCAATGCTG+TGG + chr4_2:63614852-63614871 Msa0614550:CDS 50.0%
AGATCAGGGTCAGCACAAGT+CGG - chr4_2:63615068-63615087 None:intergenic 50.0%
ACATTACACCAAGACCCCTG+AGG + chr4_2:63615640-63615659 Msa0614550:CDS 50.0%
GCAGCCAAGTCTATTCTAGC+AGG + chr4_2:63615668-63615687 Msa0614550:CDS 50.0%
!! ACAGAAGGTGCTGTCAAGCA+AGG + chr4_2:63615896-63615915 Msa0614550:CDS 50.0%
!! CAGAAGGTGCTGTCAAGCAA+GGG + chr4_2:63615897-63615916 Msa0614550:CDS 50.0%
GCTGTCAAGCAAGGGCTTAT+AGG + chr4_2:63615905-63615924 Msa0614550:CDS 50.0%
TGTCTGCACTTCAGCAAACC+AGG + chr4_2:63616039-63616058 Msa0614550:CDS 50.0%
GGCCCAATGCTAATGCTACT+AGG + chr4_2:63616164-63616183 Msa0614550:CDS 50.0%
GGCCCTAGTAGCATTAGCAT+TGG - chr4_2:63616170-63616189 None:intergenic 50.0%
AAGGCTGTTAGGCCTTGCAA+AGG - chr4_2:63616351-63616370 None:intergenic 50.0%
AATCACGGTTGCATCTGCTG+AGG - chr4_2:63616454-63616473 None:intergenic 50.0%
TAGAGGCAGAGAGTCATGAC+AGG + chr4_2:63616496-63616515 Msa0614550:CDS 50.0%
ACTAACTGTTGCTGCTGTCC+TGG - chr4_2:63616537-63616556 None:intergenic 50.0%
CTTGCCATAATGTCTGGAGG+AGG + chr4_2:63616594-63616613 Msa0614550:CDS 50.0%
CATGCCTCCTCCAGACATTA+TGG - chr4_2:63616601-63616620 None:intergenic 50.0%
CATAATGTCTGGAGGAGGCA+TGG + chr4_2:63616599-63616618 Msa0614550:CDS 50.0%
!! ATTCTGTGGGTTGGCTATCC+TGG + chr4_2:63616660-63616679 Msa0614550:CDS 50.0%
CATGAAAATTGCCCCCCACA+AGG + chr4_2:63617057-63617076 Msa0614550:intron 50.0%
GGTGTCTGTTCCTTTAGCTG+AGG + chr4_2:63617416-63617435 Msa0614550:CDS 50.0%
TGCGCCTCAGAAACACTTGT+TGG + chr4_2:63617596-63617615 Msa0614550:CDS 50.0%
GGCAACAGAAAAGTTGCCCT+AGG + chr4_2:63617711-63617730 Msa0614550:CDS 50.0%
!! AGTAGCCCGTGATGGGTGTT+AGG - chr4_2:63613919-63613938 None:intergenic 55.0%
AGCAATGCACAACGTCGGGT+TGG + chr4_2:63614606-63614625 Msa0614550:CDS 55.0%
AGGGATCCAAGATGGGGAAG+AGG + chr4_2:63614667-63614686 Msa0614550:CDS 55.0%
ACTTGGCTGCAGCTTCCTCA+GGG - chr4_2:63615658-63615677 None:intergenic 55.0%
AGCTAGCTCGTGAAGCTGCA+AGG + chr4_2:63616062-63616081 Msa0614550:CDS 55.0%
CTCGTGAAGCTGCAAGGCAA+GGG + chr4_2:63616068-63616087 Msa0614550:CDS 55.0%
GTTCCCACAAGCTTTGCTGC+TGG + chr4_2:63616372-63616391 Msa0614550:CDS 55.0%
CAGCCAGCAGCAAAGCTTGT+GGG - chr4_2:63616378-63616397 None:intergenic 55.0%
ACAGCCAGCAGCAAAGCTTG+TGG - chr4_2:63616379-63616398 None:intergenic 55.0%
GGCATCGTCTAGTGCAGCAT+TGG - chr4_2:63616418-63616437 None:intergenic 55.0%
GTTGGCTATCCTGGAGAAGC+TGG + chr4_2:63616669-63616688 Msa0614550:CDS 55.0%
GTGGAAGGCTACCCATGACA+TGG + chr4_2:63617217-63617236 Msa0614550:CDS 55.0%
!! ATGCACAACGTCGGGTTGGC+TGG + chr4_2:63614610-63614629 Msa0614550:CDS 60.0%
TGCAGCTTCCTCAGGGGTCT+TGG - chr4_2:63615651-63615670 None:intergenic 60.0%
CTTGGCTGCAGCTTCCTCAG+GGG - chr4_2:63615657-63615676 None:intergenic 60.0%
GACTTGGCTGCAGCTTCCTC+AGG - chr4_2:63615659-63615678 None:intergenic 60.0%
CAGCTTCACGAGCTAGCTCC+TGG - chr4_2:63616060-63616079 None:intergenic 60.0%
GCTCGTGAAGCTGCAAGGCA+AGG + chr4_2:63616067-63616086 Msa0614550:CDS 60.0%
GGCTATCCTGGAGAAGCTGG+TGG + chr4_2:63616672-63616691 Msa0614550:CDS 60.0%
ATGAGGCCGGATCCTGCTAC+AGG + chr4_2:63617285-63617304 Msa0614550:CDS 60.0%
GGGTAGCCTGTAGCAGGATC+CGG - chr4_2:63617294-63617313 None:intergenic 60.0%
GATCCTGCTACAGGCTACCC+TGG + chr4_2:63617294-63617313 Msa0614550:CDS 60.0%
CTACCAGGGTAGCCTGTAGC+AGG - chr4_2:63617300-63617319 None:intergenic 60.0%
TGAGGCGCATTGTGCACTGC+AGG - chr4_2:63617585-63617604 None:intergenic 60.0%
GCAGCTCCACCAGCTTCTCC+AGG - chr4_2:63616681-63616700 None:intergenic 65.0%
GGCGCATTGTGCACTGCAGG+AGG - chr4_2:63617582-63617601 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 63613798 63618000 63613798 ID=Msa0614550;Name=Msa0614550
chr4_2 mRNA 63613798 63618000 63613798 ID=Msa0614550-mRNA-1;Parent=Msa0614550;Name=Msa0614550-mRNA-1;_AED=0.07;_eAED=0.07;_QI=261|1|1|1|1|1|7|212|774
chr4_2 exon 63613798 63614493 63613798 ID=Msa0614550-mRNA-1:exon:17780;Parent=Msa0614550-mRNA-1
chr4_2 exon 63614586 63614873 63614586 ID=Msa0614550-mRNA-1:exon:17781;Parent=Msa0614550-mRNA-1
chr4_2 exon 63614957 63615126 63614957 ID=Msa0614550-mRNA-1:exon:17782;Parent=Msa0614550-mRNA-1
chr4_2 exon 63615592 63615689 63615592 ID=Msa0614550-mRNA-1:exon:17783;Parent=Msa0614550-mRNA-1
chr4_2 exon 63615858 63616208 63615858 ID=Msa0614550-mRNA-1:exon:17784;Parent=Msa0614550-mRNA-1
chr4_2 exon 63616325 63616735 63616325 ID=Msa0614550-mRNA-1:exon:17785;Parent=Msa0614550-mRNA-1
chr4_2 exon 63617217 63618000 63617217 ID=Msa0614550-mRNA-1:exon:17786;Parent=Msa0614550-mRNA-1
chr4_2 five_prime_UTR 63613798 63614058 63613798 ID=Msa0614550-mRNA-1:five_prime_utr;Parent=Msa0614550-mRNA-1
chr4_2 CDS 63614059 63614493 63614059 ID=Msa0614550-mRNA-1:cds;Parent=Msa0614550-mRNA-1
chr4_2 CDS 63614586 63614873 63614586 ID=Msa0614550-mRNA-1:cds;Parent=Msa0614550-mRNA-1
chr4_2 CDS 63614957 63615126 63614957 ID=Msa0614550-mRNA-1:cds;Parent=Msa0614550-mRNA-1
chr4_2 CDS 63615592 63615689 63615592 ID=Msa0614550-mRNA-1:cds;Parent=Msa0614550-mRNA-1
chr4_2 CDS 63615858 63616208 63615858 ID=Msa0614550-mRNA-1:cds;Parent=Msa0614550-mRNA-1
chr4_2 CDS 63616325 63616735 63616325 ID=Msa0614550-mRNA-1:cds;Parent=Msa0614550-mRNA-1
chr4_2 CDS 63617217 63617788 63617217 ID=Msa0614550-mRNA-1:cds;Parent=Msa0614550-mRNA-1
chr4_2 three_prime_UTR 63617789 63618000 63617789 ID=Msa0614550-mRNA-1:three_prime_utr;Parent=Msa0614550-mRNA-1
Gene Sequence

>Msa0614550

ATGGCCTCTGTTGAAAACAGAACACCTAACGTCTCTGTTTTCCTCTGTTTCTTTGTTCTGTTTGCCACACTTTTGCTGAGTTGTGGTCGAGTTTCGAGCCAAACATCTGCAGTTTTCGCCTGCGATGTTGCCAAGAATCCAGCATTGGCGAATTACGGGTTCTGCAACAAGAAATTGAGTGTGGATGCAAGGGTGAAAGACCTTGTGAGAAGGTTGACATTGCAAGAGAAGGTTGGTAACCTTGTGAATTCTGCTGTTGATGTAAGCAGACTTGGGATTCCAAAATATGAATGGTGGTCTGAAGCACTTCATGGAGTCTCTAACATTGGTCCAGGAACTCATTTTTCCAATGTTATACCTGGAGCAACCAGTTTTCCTATGCCTATTCTCATTGCAGCTTCTTTCAATGCTTCCTTGTTTCAAACCATAGGAAAGGTGGTTTCGACGGAAGCAAGAGCAATGCACAACGTCGGGTTGGCTGGTTTAACATATTGGTCACCAAACATTAACATATTTAGGGATCCAAGATGGGGAAGAGGTCAAGAAACACCAGGAGAAGACCCTTTACTTGCTAGCAAATATGCAGCAGGGTACGTGAAAGGTCTACAACAAACTGATGATGGAGATTCCAACAAGCTTAAGGTTGCTGCTTGTTGCAAACACTACACAGCATATGATGTTGATGACTGGAAAGGTGTTCAGCGTTACACCTTCAATGCTGTGGTGACACAACAAGATTTAGATGACACATACCAACCACCTTTTAAGAGCTGTGTGATTGATGGAAATGTTGCTAGTGTTATGTGTTCCTACAATCAAGTAAATGGAAAACCGACTTGTGCTGACCCTGATCTTCTTAAAGGTGTTATCCGTGGCAAATGGAAACTAAATGGATATATAGTCTCTGATTGTGACTCAGTAGATGTGCTTTTCAAAAATCAACATTACACCAAGACCCCTGAGGAAGCTGCAGCCAAGTCTATTCTAGCAGGATTGGATTTGAACTGTGGAAGTTTTCTTGGCCGATATACAGAAGGTGCTGTCAAGCAAGGGCTTATAGGTGAAGCATCCATTAACAATGCCGTCTACAATAATTTTGCGACATTAATGCGTCTAGGTTTCTTTGATGGCGATCCTAGCAAGCAACCTTATGGAAACCTTGGTCCAAAAGATGTCTGCACTTCAGCAAACCAGGAGCTAGCTCGTGAAGCTGCAAGGCAAGGGATTGTGTTGCTTAAAAACTGTGCAGGATCATTGCCTCTGAATGCCAAAGCTATTAAATCATTGGCAGTTATTGGGCCCAATGCTAATGCTACTAGGGCCATGATTGGAAACTACGAAGGCATTCCATGCAAATATACATCACCTTTGCAAGGCCTAACAGCCTTAGTTCCCACAAGCTTTGCTGCTGGCTGTCCTGATGTTCAATGCACCAATGCTGCACTAGACGATGCCAAAAAGATTGCAGCCTCAGCAGATGCAACCGTGATTGTAGTCGGTGCAAATCTTGCAATAGAGGCAGAGAGTCATGACAGGATTAACATACTTCTTCCAGGACAGCAGCAACAGTTAGTTACAGAAGTTGCAAATGTAGCTAAGGGTCCTGTGATTCTTGCCATAATGTCTGGAGGAGGCATGGATGTTTCCTTTGCTAAAACTAATAAAAAGATCACAAGCATTCTGTGGGTTGGCTATCCTGGAGAAGCTGGTGGAGCTGCCATAGCTGATGTCATCTTTGGGTATCATAATCCAAGTGGAAGGCTACCCATGACATGGTATCCACAATCATATGTAGACAAAGTACCAATGACCAATATGAACATGAGGCCGGATCCTGCTACAGGCTACCCTGGTAGAACATACAGATTTTATAAAGGGGAAACTGTCTTCTCATTTGGAGATGGAATAAGCTACTCAACTTTCGAGCATAAATTAGTTAAAGCACCACAATTGGTGTCTGTTCCTTTAGCTGAGGATCATGTTTGTCGTTCCTCAAAATGTAAGTCACTTGATGTTGTTGGCGAACATTGTCAAAACTTAGCCTTTGATATTCACCTCAGGATCAAGAACAAAGGGAAAATGAGCAGTAGCCAAACAGTGTTTTTGTTCTCTACTCCTCCTGCAGTGCACAATGCGCCTCAGAAACACTTGTTGGCTTTCGAGAAAGTACACTTGACAGGAAAATCAGAAGCACTGGTCAGTTTCAAAGTAGATGTTTGTAATGATTTGAGTGTGGTTGATGAACTTGGCAACAGAAAAGTTGCCCTAGGAAAACATATGCTTCATGTAGGAGACTTGAAGCATCCTTTGAGCGTGATGATTTAA
Protein sequence

>Msa0614550

MASVENRTPNVSVFLCFFVLFATLLLSCGRVSSQTSAVFACDVAKNPALANYGFCNKKLSVDARVKDLVRRLTLQEKVGNLVNSAVDVSRLGIPKYEWWSEALHGVSNIGPGTHFSNVIPGATSFPMPILIAASFNASLFQTIGKVVSTEARAMHNVGLAGLTYWSPNINIFRDPRWGRGQETPGEDPLLASKYAAGYVKGLQQTDDGDSNKLKVAACCKHYTAYDVDDWKGVQRYTFNAVVTQQDLDDTYQPPFKSCVIDGNVASVMCSYNQVNGKPTCADPDLLKGVIRGKWKLNGYIVSDCDSVDVLFKNQHYTKTPEEAAAKSILAGLDLNCGSFLGRYTEGAVKQGLIGEASINNAVYNNFATLMRLGFFDGDPSKQPYGNLGPKDVCTSANQELAREAARQGIVLLKNCAGSLPLNAKAIKSLAVIGPNANATRAMIGNYEGIPCKYTSPLQGLTALVPTSFAAGCPDVQCTNAALDDAKKIAASADATVIVVGANLAIEAESHDRINILLPGQQQQLVTEVANVAKGPVILAIMSGGGMDVSFAKTNKKITSILWVGYPGEAGGAAIADVIFGYHNPSGRLPMTWYPQSYVDKVPMTNMNMRPDPATGYPGRTYRFYKGETVFSFGDGISYSTFEHKLVKAPQLVSVPLAEDHVCRSSKCKSLDVVGEHCQNLAFDIHLRIKNKGKMSSSQTVFLFSTPPAVHNAPQKHLLAFEKVHLTGKSEALVSFKVDVCNDLSVVDELGNRKVALGKHMLHVGDLKHPLSVMI*