AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1466920


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1466920 MtrunA17_Chr7g0273351 100.000 66 0 0 1 66 1 66 1.11e-40 129
Msa1466920 MtrunA17_Chr7g0273331 100.000 66 0 0 1 66 57 122 7.77e-40 129
Msa1466920 MtrunA17_Chr7g0268831 92.754 69 2 1 1 66 1 69 3.59e-37 120
Msa1466920 MtrunA17_Chr7g0273341 95.082 61 3 0 6 66 45 105 4.59e-35 116
Msa1466920 MtrunA17_Chr7g0259151 83.607 61 10 0 6 66 9 69 2.37e-31 105
Msa1466920 MtrunA17_Chr2g0319191 83.607 61 6 1 10 66 3 63 2.49e-27 95.5
Msa1466920 MtrunA17_Chr2g0319181 82.540 63 3 2 10 66 3 63 7.64e-27 94.4
Msa1466920 MtrunA17_Chr7g0272611 72.414 58 16 0 9 66 16 73 5.50e-24 87.4
Msa1466920 MtrunA17_Chr2g0327951 79.592 49 10 0 18 66 58 106 1.05e-22 85.1
Msa1466920 MtrunA17_Chr4g0031041 79.592 49 10 0 18 66 17 65 2.35e-22 83.2
Msa1466920 MtrunA17_Chr4g0031001 79.167 48 10 0 19 66 24 71 8.65e-22 82.0
Msa1466920 MtrunA17_Chr4g0031651 79.167 48 10 0 19 66 24 71 8.74e-22 82.0
Msa1466920 MtrunA17_Chr4g0035621 79.167 48 10 0 19 66 24 71 8.93e-22 82.0
Msa1466920 MtrunA17_Chr4g0032301 79.167 48 10 0 19 66 24 71 1.20e-21 81.6
Msa1466920 MtrunA17_Chr2g0327931 79.167 48 10 0 19 66 23 70 1.20e-21 81.6
Msa1466920 MtrunA17_Chr8g0379311 79.167 48 10 0 19 66 24 71 1.31e-21 81.6
Msa1466920 MtrunA17_Chr6g0449661 67.442 43 14 0 19 61 85 127 7.77e-17 70.5
Msa1466920 MtrunA17_Chr3g0119971 69.048 42 13 0 20 61 81 122 8.18e-17 70.1
Msa1466920 MtrunA17_Chr1g0148961 55.385 65 28 1 3 66 10 74 1.55e-14 62.8
Msa1466920 MtrunA17_Chr1g0148951 55.385 65 28 1 3 66 10 74 1.55e-14 62.8
Msa1466920 MtrunA17_Chr1g0211651 55.932 59 24 2 10 66 13 71 6.32e-13 58.9
Msa1466920 MtrunA17_Chr1g0184901 65.957 47 15 1 21 66 28 74 1.12e-12 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1466920 AT1G54690.1 92.754 69 2 1 1 66 1 69 2.80e-37 121
Msa1466920 AT1G08880.1 95.238 63 3 0 4 66 7 69 7.92e-37 120
Msa1466920 AT4G27230.2 85.246 61 5 1 10 66 3 63 1.68e-27 96.3
Msa1466920 AT4G27230.1 85.246 61 5 1 10 66 3 63 1.68e-27 96.3
Msa1466920 AT5G54640.1 78.788 66 11 1 1 66 1 63 4.47e-27 95.1
Msa1466920 AT1G51060.1 80.328 61 8 1 10 66 3 63 4.73e-26 92.4
Msa1466920 AT3G20670.1 81.967 61 7 1 10 66 3 63 9.72e-26 91.7
Msa1466920 AT5G59870.1 69.014 71 17 2 1 66 1 71 1.12e-24 89.7
Msa1466920 AT5G27670.1 68.571 70 17 1 2 66 3 72 1.08e-22 84.7
Msa1466920 AT5G02560.1 77.551 49 11 0 18 66 23 71 2.06e-21 81.3
Msa1466920 AT5G02560.2 77.551 49 11 0 18 66 23 71 4.43e-21 81.3
Msa1466920 AT2G38810.1 64.000 50 17 1 18 66 27 76 2.11e-13 60.5
Msa1466920 AT2G38810.4 64.000 50 17 1 18 66 27 76 2.11e-13 60.5
Msa1466920 AT2G38810.3 64.000 50 17 1 18 66 27 76 2.11e-13 60.5
Msa1466920 AT2G38810.2 64.000 50 17 1 18 66 27 76 2.11e-13 60.5
Msa1466920 AT1G52740.1 62.500 48 17 1 20 66 27 74 4.13e-13 59.7
Msa1466920 AT3G54560.1 60.417 48 18 1 20 66 29 76 1.29e-11 55.8
Msa1466920 AT3G54560.2 60.417 48 18 1 20 66 29 76 1.29e-11 55.8

Find 29 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACTGATTTGGCAGCCTT+TGG 0.309618 tig0049272:+168 Msa1466920:intergenic
TGAAGATCTTGAAACTGATT+TGG 0.311823 tig0049272:+158 Msa1466920:intergenic
AACACGTTCGGCGTATTTAC+CGG 0.321195 tig0049272:+86 Msa1466920:intergenic
GAGTTCCACTGGCACAACTA+AGG 0.456467 tig0049272:-205 Msa1466920:CDS
AGGACGGCGGAGAGGTAGAC+AGG 0.463287 tig0049272:+51 Msa1466920:intergenic
AGCACCGGCACCAACACGTT+CGG 0.480590 tig0049272:+74 Msa1466920:intergenic
GTTTCAAGATCTTCAAAGGC+AGG 0.496108 tig0049272:-150 Msa1466920:CDS
CTGGCACAACTAAGGGCGGA+AGG 0.502885 tig0049272:-197 Msa1466920:CDS
ATCAGTTTCAAGATCTTCAA+AGG 0.503488 tig0049272:-154 Msa1466920:CDS
GGCACAACTAAGGGCGGAAG+GGG 0.525782 tig0049272:-195 Msa1466920:CDS
TGGCACAACTAAGGGCGGAA+GGG 0.543224 tig0049272:-196 Msa1466920:CDS
AGTTCCACTGGCACAACTAA+GGG 0.549244 tig0049272:-204 Msa1466920:CDS
TACGCCGAACGTGTTGGTGC+CGG 0.556200 tig0049272:-78 Msa1466920:CDS
GGGCGGAAGGGGTAAGCCAA+AGG 0.573881 tig0049272:-184 Msa1466920:CDS
CGGCGTATTTACCGGCCTTG+AGG 0.577173 tig0049272:+94 Msa1466920:intergenic
ATCGCGAGATTCCTCAAGGC+CGG 0.578871 tig0049272:-105 Msa1466920:CDS
TCCGCCCTTAGTTGTGCCAG+TGG 0.580065 tig0049272:+200 Msa1466920:intergenic
GAATCTCGCGATTCTTCCGA+CGG 0.592664 tig0049272:+116 Msa1466920:intergenic
GGTAAATACGCCGAACGTGT+TGG 0.593895 tig0049272:-84 Msa1466920:CDS
CCTTGAGTATCTCGCTGCTG+AGG 0.612329 tig0049272:-31 None:intergenic
AATCTCGCGATTCTTCCGAC+GGG 0.626786 tig0049272:+117 Msa1466920:intergenic
CCTCAGCAGCGAGATACTCA+AGG 0.638807 tig0049272:+31 Msa1466920:intergenic
GGAGAGGTAGACAGGAGCAC+CGG 0.644805 tig0049272:+59 Msa1466920:intergenic
TCCACTGGCACAACTAAGGG+CGG 0.647888 tig0049272:-201 Msa1466920:CDS
AAGAATCGCGAGATTCCTCA+AGG 0.651972 tig0049272:-109 Msa1466920:CDS
GCAGGTCTTCAATTTCCCGT+CGG 0.653866 tig0049272:-132 Msa1466920:CDS
GATACTCAAGGACGGCGGAG+AGG 0.669935 tig0049272:+43 Msa1466910:intergenic
AGCAGCGAGATACTCAAGGA+CGG 0.684917 tig0049272:+35 Msa1466910:intergenic
AGCGAGATACTCAAGGACGG+CGG 0.736224 tig0049272:+38 Msa1466910:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TGAAGATCTTGAAACTGATT+TGG + tig0049272:84-103 Msa1466920:intergenic 30.0%
ATCAGTTTCAAGATCTTCAA+AGG - tig0049272:85-104 Msa1466920:CDS 30.0%
GTTTCAAGATCTTCAAAGGC+AGG - tig0049272:89-108 Msa1466920:CDS 40.0%
AGTTCCACTGGCACAACTAA+GGG - tig0049272:35-54 Msa1466920:CDS 45.0%
!! GAAACTGATTTGGCAGCCTT+TGG + tig0049272:74-93 Msa1466920:intergenic 45.0%
AAGAATCGCGAGATTCCTCA+AGG - tig0049272:130-149 Msa1466920:CDS 45.0%
AACACGTTCGGCGTATTTAC+CGG + tig0049272:156-175 Msa1466920:intergenic 45.0%
GAGTTCCACTGGCACAACTA+AGG - tig0049272:34-53 Msa1466920:CDS 50.0%
GCAGGTCTTCAATTTCCCGT+CGG - tig0049272:107-126 Msa1466920:CDS 50.0%
AATCTCGCGATTCTTCCGAC+GGG + tig0049272:125-144 Msa1466920:intergenic 50.0%
GAATCTCGCGATTCTTCCGA+CGG + tig0049272:126-145 Msa1466920:intergenic 50.0%
GGTAAATACGCCGAACGTGT+TGG - tig0049272:155-174 Msa1466920:CDS 50.0%
AGCAGCGAGATACTCAAGGA+CGG + tig0049272:207-226 Msa1466920:intergenic 50.0%
TCCACTGGCACAACTAAGGG+CGG - tig0049272:38-57 Msa1466920:CDS 55.0%
TGGCACAACTAAGGGCGGAA+GGG - tig0049272:43-62 Msa1466920:CDS 55.0%
ATCGCGAGATTCCTCAAGGC+CGG - tig0049272:134-153 Msa1466920:CDS 55.0%
AGCGAGATACTCAAGGACGG+CGG + tig0049272:204-223 Msa1466920:intergenic 55.0%
TCCGCCCTTAGTTGTGCCAG+TGG + tig0049272:42-61 Msa1466920:intergenic 60.0%
CTGGCACAACTAAGGGCGGA+AGG - tig0049272:42-61 Msa1466920:CDS 60.0%
GGCACAACTAAGGGCGGAAG+GGG - tig0049272:44-63 Msa1466920:CDS 60.0%
CGGCGTATTTACCGGCCTTG+AGG + tig0049272:148-167 Msa1466920:intergenic 60.0%
! TACGCCGAACGTGTTGGTGC+CGG - tig0049272:161-180 Msa1466920:CDS 60.0%
!! AGCACCGGCACCAACACGTT+CGG + tig0049272:168-187 Msa1466920:intergenic 60.0%
! GGAGAGGTAGACAGGAGCAC+CGG + tig0049272:183-202 Msa1466920:intergenic 60.0%
GATACTCAAGGACGGCGGAG+AGG + tig0049272:199-218 Msa1466920:intergenic 60.0%
GGGCGGAAGGGGTAAGCCAA+AGG - tig0049272:55-74 Msa1466920:CDS 65.0%
AGGACGGCGGAGAGGTAGAC+AGG + tig0049272:191-210 Msa1466920:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0049272 gene 32 229 32 ID=Msa1466920;Name=Msa1466920
tig0049272 mRNA 32 229 32 ID=Msa1466920-mRNA-1;Parent=Msa1466920;Name=Msa1466920-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|-1|0|1|-1|1|1|0|66
tig0049272 exon 32 229 32 ID=Msa1466920-mRNA-1:exon:25168;Parent=Msa1466920-mRNA-1
tig0049272 CDS 32 229 32 ID=Msa1466920-mRNA-1:cds;Parent=Msa1466920-mRNA-1
Gene Sequence

>Msa1466920

ATGAGTTCCACTGGCACAACTAAGGGCGGAAGGGGTAAGCCAAAGGCTGCCAAATCAGTTTCAAGATCTTCAAAGGCAGGTCTTCAATTTCCCGTCGGAAGAATCGCGAGATTCCTCAAGGCCGGTAAATACGCCGAACGTGTTGGTGCCGGTGCTCCTGTCTACCTCTCCGCCGTCCTTGAGTATCTCGCTGCTGAG

Protein sequence

>Msa1466920

MSSTGTTKGGRGKPKAAKSVSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLSAVLEYLAAE