AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1267900


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1267900 MtrunA17_Chr8g0379151 93.902 82 5 0 14 95 299 380 3.45e-52 167
Msa1267900 MtrunA17_Chr2g0283741 53.086 81 36 1 8 88 281 359 1.10e-22 89.7
Msa1267900 MtrunA17_Chr2g0283901 43.023 86 46 2 3 88 274 356 1.39e-17 75.9
Msa1267900 MtrunA17_Chr3g0142071 58.621 58 22 1 33 90 303 358 1.59e-16 72.8
Msa1267900 MtrunA17_Chr7g0248371 45.455 88 38 5 1 87 53 131 1.14e-13 62.4
Msa1267900 MtrunA17_Chr4g0041481 43.902 82 40 3 5 86 263 338 4.57e-13 63.2
Msa1267900 MtrunA17_Chr4g0053311 48.387 62 31 1 33 94 288 348 6.87e-13 62.4
Msa1267900 MtrunA17_Chr4g0041521 43.902 82 40 3 5 86 263 338 1.25e-12 61.6
Msa1267900 MtrunA17_Chr3g0116551 42.857 84 41 4 8 90 270 347 1.39e-12 61.6
Msa1267900 MtrunA17_Chr7g0248391 45.455 88 38 4 1 87 259 337 1.73e-12 61.2
Msa1267900 MtrunA17_Chr2g0325121 42.683 82 41 3 7 88 262 337 2.26e-12 60.8
Msa1267900 MtrunA17_Chr7g0248661 43.210 81 39 4 8 87 2 76 5.90e-12 56.2
Msa1267900 MtrunA17_Chr1g0208261 49.091 55 27 1 34 88 343 396 7.44e-12 59.7
Msa1267900 MtrunA17_Chr4g0040851 39.506 81 43 3 7 87 285 359 8.95e-12 59.3
Msa1267900 MtrunA17_Chr1g0153011 51.852 54 25 1 35 88 291 343 9.37e-12 59.3
Msa1267900 MtrunA17_Chr4g0041471 42.857 84 41 4 5 87 264 341 9.90e-12 59.3
Msa1267900 MtrunA17_Chr3g0134231 53.704 54 24 1 35 88 310 362 1.21e-11 58.9
Msa1267900 MtrunA17_Chr4g0060251 40.506 79 42 2 8 86 270 343 2.46e-11 58.2
Msa1267900 MtrunA17_Chr4g0041061 40.506 79 42 2 8 86 270 343 5.50e-11 57.0
Msa1267900 MtrunA17_Chr4g0040881 40.506 79 42 2 8 86 270 343 7.38e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1267900 AT1G29110.1 44.444 81 40 2 7 87 258 333 1.33e-18 78.6
Msa1267900 AT4G23520.1 56.452 62 25 2 33 94 297 356 8.99e-17 73.9
Msa1267900 AT1G09850.1 45.977 87 41 3 3 89 255 335 2.51e-16 72.8
Msa1267900 AT3G19400.1 46.032 63 32 1 33 95 296 356 2.55e-15 69.7
Msa1267900 AT2G27420.1 43.182 88 43 4 1 87 266 347 2.60e-14 67.0
Msa1267900 AT4G35350.1 56.364 55 22 1 35 89 302 354 4.95e-14 66.2
Msa1267900 AT3G43960.1 51.562 64 28 2 33 95 293 354 6.72e-14 65.9
Msa1267900 AT1G29080.1 41.975 81 41 3 8 87 270 345 1.01e-13 65.1
Msa1267900 AT4G11320.2 41.935 93 45 4 4 93 279 365 1.06e-13 65.1
Msa1267900 AT4G11320.1 41.935 93 45 4 4 93 279 365 1.06e-13 65.1
Msa1267900 AT1G20850.1 56.364 55 22 1 35 89 303 355 3.32e-13 63.9
Msa1267900 AT5G43060.1 51.786 56 25 1 33 88 301 354 4.28e-13 63.5
Msa1267900 AT4G11310.1 40.698 86 45 3 8 93 279 358 6.08e-13 63.2
Msa1267900 AT3G48340.1 49.180 61 29 1 35 95 293 351 9.61e-13 62.4
Msa1267900 AT1G47128.1 53.571 56 24 1 33 88 300 353 1.13e-12 62.4
Msa1267900 AT2G34080.1 43.210 81 39 4 8 87 270 344 2.38e-12 61.2
Msa1267900 AT3G19390.1 46.552 58 29 1 33 90 293 348 4.05e-12 60.8
Msa1267900 AT4G36880.1 40.741 81 43 3 8 88 287 362 4.28e-12 60.8
Msa1267900 AT5G50260.1 44.828 58 29 2 34 90 290 345 8.44e-12 59.7
Msa1267900 AT3G48350.1 49.123 57 26 2 33 88 290 344 1.75e-11 58.9
Msa1267900 AT1G29090.1 40.741 81 42 3 8 87 279 354 2.21e-11 58.5
Msa1267900 AT3G49340.1 35.632 87 51 2 1 87 259 340 3.72e-11 58.2
Msa1267900 AT5G60360.1 35.802 81 46 1 7 87 282 356 5.80e-11 57.4
Msa1267900 AT1G06260.1 47.273 55 27 1 34 88 291 343 7.50e-11 57.0

Find 23 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGTGAATGGTGAAGACTAT+TGG 0.304070 8_3:+239371 Msa1267900:CDS
GTTATTGAAGATTGGCCTTA+TGG 0.314718 8_3:+239457 Msa1267900:CDS
ATCTTTCAAAAGGTTGAATC+TGG 0.348902 8_3:+239178 Msa1267900:CDS
AAAGGAATGTTATTGAAGAT+TGG 0.377330 8_3:+239449 Msa1267900:CDS
TGACTAAAATAGGGAGTCTA+TGG 0.386085 8_3:+239283 Msa1267900:intron
AAACTCATGGGGAAAAGATT+GGG 0.397399 8_3:+239402 Msa1267900:CDS
AAGGTTACTTGTTTATCAAA+AGG 0.408095 8_3:+239431 Msa1267900:CDS
TCAAGGAATGCAAATCTTGT+TGG 0.438289 8_3:+239328 Msa1267900:CDS
AAAACTCATGGGGAAAAGAT+TGG 0.442756 8_3:+239401 Msa1267900:CDS
TCTAATCGTGGGTTATGATT+CGG 0.463790 8_3:+239351 Msa1267900:CDS
GTGTCGATATATCTTTCAAA+AGG 0.514366 8_3:+239168 Msa1267900:CDS
GGAAAAGATTGGGGAGAGAA+AGG 0.518533 8_3:+239412 Msa1267900:CDS
CTTTATCTTCAGCCATGTAG+TGG 0.532318 8_3:+239524 Msa1267900:CDS
TTGATTGCACATACACCATA+AGG 0.533070 8_3:-239472 None:intergenic
GTGGGTTATGATTCGGTGAA+TGG 0.534697 8_3:+239358 Msa1267900:CDS
TGCTCTAACCAACCACTACA+TGG 0.537247 8_3:-239536 None:intergenic
AATCTTGTTGGTCTAATCGT+GGG 0.553988 8_3:+239340 Msa1267900:CDS
AAATCTTGTTGGTCTAATCG+TGG 0.581439 8_3:+239339 Msa1267900:CDS
CTAAAATAGGGAGTCTATGG+TGG 0.604269 8_3:+239286 Msa1267900:intron
AACTCATGGGGAAAAGATTG+GGG 0.629466 8_3:+239403 Msa1267900:CDS
TGTGCAATCAATGCTGCGGT+TGG 0.646120 8_3:+239484 Msa1267900:CDS
TAAAGTTTAGTGCTAATGCG+AGG 0.703141 8_3:+239146 None:intergenic
TGTATGTGCAATCAATGCTG+CGG 0.774207 8_3:+239480 Msa1267900:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGTTGTTTTGACTAAAAT+AGG + chr8_3:239273-239292 Msa1267900:intron 20.0%
!!! TTGTTGTTTTGACTAAAATA+GGG + chr8_3:239274-239293 Msa1267900:intron 20.0%
! AAGGTTACTTGTTTATCAAA+AGG + chr8_3:239431-239450 Msa1267900:CDS 25.0%
! AAAGGAATGTTATTGAAGAT+TGG + chr8_3:239449-239468 Msa1267900:CDS 25.0%
!!! AAGATAAAGCTGTTTTGATT+GGG - chr8_3:239513-239532 None:intergenic 25.0%
! GTGTCGATATATCTTTCAAA+AGG + chr8_3:239168-239187 Msa1267900:CDS 30.0%
ATCTTTCAAAAGGTTGAATC+TGG + chr8_3:239178-239197 Msa1267900:CDS 30.0%
AGAACTGCAAAAAAGATTCA+AGG + chr8_3:239311-239330 Msa1267900:CDS 30.0%
ATTGGATTGTGAAAAACTCA+TGG + chr8_3:239389-239408 Msa1267900:CDS 30.0%
TTGGATTGTGAAAAACTCAT+GGG + chr8_3:239390-239409 Msa1267900:CDS 30.0%
!!! GAAGATAAAGCTGTTTTGAT+TGG - chr8_3:239514-239533 None:intergenic 30.0%
TGACTAAAATAGGGAGTCTA+TGG + chr8_3:239283-239302 Msa1267900:intron 35.0%
TCAAGGAATGCAAATCTTGT+TGG + chr8_3:239328-239347 Msa1267900:CDS 35.0%
! AAATCTTGTTGGTCTAATCG+TGG + chr8_3:239339-239358 Msa1267900:CDS 35.0%
! AATCTTGTTGGTCTAATCGT+GGG + chr8_3:239340-239359 Msa1267900:CDS 35.0%
! TCTAATCGTGGGTTATGATT+CGG + chr8_3:239351-239370 Msa1267900:CDS 35.0%
TGGATTGTGAAAAACTCATG+GGG + chr8_3:239391-239410 Msa1267900:CDS 35.0%
AAAACTCATGGGGAAAAGAT+TGG + chr8_3:239401-239420 Msa1267900:CDS 35.0%
AAACTCATGGGGAAAAGATT+GGG + chr8_3:239402-239421 Msa1267900:CDS 35.0%
!! GTTATTGAAGATTGGCCTTA+TGG + chr8_3:239457-239476 Msa1267900:CDS 35.0%
TTGATTGCACATACACCATA+AGG - chr8_3:239475-239494 None:intergenic 35.0%
CTAAAATAGGGAGTCTATGG+TGG + chr8_3:239286-239305 Msa1267900:intron 40.0%
AACTCATGGGGAAAAGATTG+GGG + chr8_3:239403-239422 Msa1267900:CDS 40.0%
TGTATGTGCAATCAATGCTG+CGG + chr8_3:239480-239499 Msa1267900:CDS 40.0%
!! GTGGGTTATGATTCGGTGAA+TGG + chr8_3:239358-239377 Msa1267900:CDS 45.0%
CGGTGAATGGTGAAGACTAT+TGG + chr8_3:239371-239390 Msa1267900:CDS 45.0%
GGAAAAGATTGGGGAGAGAA+AGG + chr8_3:239412-239431 Msa1267900:CDS 45.0%
TGTGCAATCAATGCTGCGGT+TGG + chr8_3:239484-239503 Msa1267900:CDS 50.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 239161 239543 239161 ID=Msa1267900;Name=Msa1267900
chr8_3 mRNA 239161 239543 239161 ID=Msa1267900-mRNA-1;Parent=Msa1267900;Name=Msa1267900-mRNA-1;_AED=0.11;_eAED=0.19;_QI=0|0|0|0.5|1|1|2|0|95
chr8_3 exon 239161 239199 239161 ID=Msa1267900-mRNA-1:exon:45;Parent=Msa1267900-mRNA-1
chr8_3 exon 239295 239543 239295 ID=Msa1267900-mRNA-1:exon:46;Parent=Msa1267900-mRNA-1
chr8_3 CDS 239161 239199 239161 ID=Msa1267900-mRNA-1:cds;Parent=Msa1267900-mRNA-1
chr8_3 CDS 239295 239543 239295 ID=Msa1267900-mRNA-1:cds;Parent=Msa1267900-mRNA-1
Gene Sequence

>Msa1267900

ATGCGAGGTGTCGATATATCTTTCAAAAGGTTGAATCTGGGAGTCTATGGTGGTGAGAACTGCAAAAAAGATTCAAGGAATGCAAATCTTGTTGGTCTAATCGTGGGTTATGATTCGGTGAATGGTGAAGACTATTGGATTGTGAAAAACTCATGGGGAAAAGATTGGGGAGAGAAAGGTTACTTGTTTATCAAAAGGAATGTTATTGAAGATTGGCCTTATGGTGTATGTGCAATCAATGCTGCGGTTGGTTACCCAATCAAAACAGCTTTATCTTCAGCCATGTAG

Protein sequence

>Msa1267900

MRGVDISFKRLNLGVYGGENCKKDSRNANLVGLIVGYDSVNGEDYWIVKNSWGKDWGEKGYLFIKRNVIEDWPYGVCAINAAVGYPIKTALSSAM*