AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0985290


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 76 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGAGTAGACGGGTGGAGG+TGG 0.300627 6_4:-31623265 Msa0985290:three_prime_UTR
GGTGAGTAGACGGGTGGAGG+TGG 0.300627 6_4:-31623367 Msa0985290:three_prime_UTR
GGTGAGTAGACGGGTGGAGG+AGG 0.319166 6_4:-31623316 Msa0985290:three_prime_UTR
GGTGAGTAGACGGGTGGAGG+TGG 0.322204 6_4:-31623418 Msa0985290:three_prime_UTR
GGTGACTTGTACTTGTAAAC+TGG 0.357024 6_4:-31623550 Msa0985290:three_prime_UTR
TCCTCCTTACCACTACTAAA+TGG 0.365236 6_4:+31623678 None:intergenic
GGTGAGTAAGCAGGAGGAGG+AGG 0.413816 6_4:-31623622 Msa0985290:exon
GGTGGTGAGTAGACGGGTGG+AGG 0.432340 6_4:-31623319 Msa0985290:three_prime_UTR
GGTGGTGAGTAGACGGGTGG+AGG 0.441074 6_4:-31623268 Msa0985290:three_prime_UTR
GGTGGTGAGTAGACGGGTGG+AGG 0.441074 6_4:-31623370 Msa0985290:three_prime_UTR
GGAGACTTGTATTTGTAAAG+TGG 0.445599 6_4:-31623448 Msa0985290:three_prime_UTR
GGAGACTTATACTTGTAAAC+TGG 0.445660 6_4:-31623601 Msa0985290:three_prime_UTR
GGTGAGTAGACAGGTGGAGG+AGG 0.447436 6_4:-31623469 Msa0985290:intron
GGGGACTTGTACTTGTAAAC+TGG 0.458453 6_4:-31623295 Msa0985290:three_prime_UTR
GGGGACTTGTACTTGTAAAC+TGG 0.460087 6_4:-31623244 None:intergenic
GGGGACTTGTACTTGTAAAC+TGG 0.460087 6_4:-31623346 Msa0985290:three_prime_UTR
GGGGACTTGTACTTGTAAAC+TGG 0.460087 6_4:-31623397 Msa0985290:three_prime_UTR
GGTGGAGGTGGTGAGTAGAC+GGG 0.461122 6_4:-31623427 Msa0985290:three_prime_UTR
GTGAGTAGACGGGTGGAGGT+GGG 0.465366 6_4:-31623264 Msa0985290:three_prime_UTR
GTGAGTAGACGGGTGGAGGT+GGG 0.465366 6_4:-31623366 Msa0985290:three_prime_UTR
GTGAGTAGACGGGTGGAGGT+GGG 0.465366 6_4:-31623417 Msa0985290:three_prime_UTR
GGTGGTGAGTAGACGGGTGG+AGG 0.466798 6_4:-31623421 Msa0985290:three_prime_UTR
GGTGGAGGTGGTGAGTAAGC+AGG 0.475649 6_4:-31623631 Msa0985290:exon
GGTGGTGAGTAAGCAGGAGG+AGG 0.480025 6_4:-31623625 Msa0985290:exon
TACTTGTAAACTGGTGGTGG+TGG 0.483654 6_4:-31623286 Msa0985290:three_prime_UTR
TACTTGTAAACTGGTGGTGG+TGG 0.483654 6_4:-31623388 Msa0985290:three_prime_UTR
GGTGAATAGACAGGAGGAGG+TGG 0.493408 6_4:-31623571 Msa0985290:three_prime_UTR
GACTTGTATTTGTAAAGTGG+TGG 0.505674 6_4:-31623445 Msa0985290:three_prime_UTR
TGGTGGAGGTGGTGAGTAGA+CGG 0.505748 6_4:-31623428 Msa0985290:three_prime_UTR
TGAGTAGACGGGTGGAGGTG+GGG 0.516214 6_4:-31623263 Msa0985290:three_prime_UTR
TGAGTAGACGGGTGGAGGTG+GGG 0.516214 6_4:-31623365 Msa0985290:three_prime_UTR
TGAGTAGACGGGTGGAGGTG+GGG 0.516214 6_4:-31623416 Msa0985290:three_prime_UTR
TACTTGTAAACTGGTGGAGG+TGG 0.518842 6_4:-31623337 Msa0985290:three_prime_UTR
TAGATATAATGTGGTGGAGG+TGG 0.521642 6_4:-31623643 Msa0985290:exon
GTGACTTGTACTTGTAAACT+GGG 0.523791 6_4:-31623549 Msa0985290:three_prime_UTR
GGTGGTGAATAGACAGGAGG+AGG 0.525145 6_4:-31623574 Msa0985290:three_prime_UTR
GTGAGTAGACGGGTGGAGGA+GGG 0.525831 6_4:-31623315 Msa0985290:three_prime_UTR
TGAGTAGACGGGTGGAGGAG+GGG 0.526670 6_4:-31623314 Msa0985290:three_prime_UTR
GGTGGTGGTGGTGAGTAGAC+GGG 0.529501 6_4:-31623274 Msa0985290:three_prime_UTR
GGTGGTGGTGGTGAGTAGAC+GGG 0.529501 6_4:-31623376 Msa0985290:three_prime_UTR
GGTGGAGGTGGTGAGTAGAC+GGG 0.530290 6_4:-31623325 Msa0985290:three_prime_UTR
TTGTACTTGTAAACTGGTGG+TGG 0.532190 6_4:-31623289 Msa0985290:three_prime_UTR
TTGTACTTGTAAACTGGTGG+TGG 0.532190 6_4:-31623391 Msa0985290:three_prime_UTR
TAGTAGTGGTAAGGAGGAGG+TGG 0.537644 6_4:-31623673 Msa0985290:five_prime_UTR
TGGTGGTGGTGGTGAGTAGA+CGG 0.545412 6_4:-31623275 Msa0985290:three_prime_UTR
TGGTGGTGGTGGTGAGTAGA+CGG 0.545412 6_4:-31623377 Msa0985290:three_prime_UTR
TACTTGTAAACTGGTGGAGG+TGG 0.545632 6_4:-31623592 Msa0985290:three_prime_UTR
ACCACCATTTAGTAGTGGTA+AGG 0.552807 6_4:-31623682 Msa0985290:five_prime_UTR
TGGTGGAGGTGGTGAGTAGA+CGG 0.560893 6_4:-31623326 Msa0985290:three_prime_UTR
GGTGGAGGTGGTGAATAGAC+AGG 0.569201 6_4:-31623580 Msa0985290:three_prime_UTR
TATTTGTAAAGTGGTGGAGG+TGG 0.570617 6_4:-31623439 Msa0985290:three_prime_UTR
GACTTGTACTTGTAAACTGG+TGG 0.575994 6_4:-31623394 Msa0985290:three_prime_UTR
GACTTATACTTGTAAACTGG+TGG 0.580181 6_4:-31623598 Msa0985290:three_prime_UTR
GGTGGTGGTGAGTAGACGGG+TGG 0.586082 6_4:-31623271 Msa0985290:three_prime_UTR
GGTGGTGGTGAGTAGACGGG+TGG 0.586082 6_4:-31623373 Msa0985290:three_prime_UTR
TTATACTTGTAAACTGGTGG+AGG 0.588467 6_4:-31623595 Msa0985290:three_prime_UTR
TTGTATTTGTAAAGTGGTGG+AGG 0.594079 6_4:-31623442 Msa0985290:three_prime_UTR
GAGTAGATATAATGTGGTGG+AGG 0.594587 6_4:-31623646 Msa0985290:exon
GGAGGTGGTGAGTAAGCAGG+AGG 0.598069 6_4:-31623628 Msa0985290:exon
TTGTACTTGTAAACTGGTGG+AGG 0.598373 6_4:-31623340 Msa0985290:three_prime_UTR
GACTTGTACTTGTAAACTGG+TGG 0.601010 6_4:-31623343 Msa0985290:three_prime_UTR
ATTTAGTAGTGGTAAGGAGG+AGG 0.602889 6_4:-31623676 Msa0985290:five_prime_UTR
GGAGGTGGTGAGTAGACGGG+TGG 0.603335 6_4:-31623322 Msa0985290:three_prime_UTR
GGAGGTGGTGAGTAGACGGG+TGG 0.603335 6_4:-31623424 Msa0985290:three_prime_UTR
ACCATTTAGTAGTGGTAAGG+AGG 0.618262 6_4:-31623679 Msa0985290:five_prime_UTR
CGTAAACCACCATTTAGTAG+TGG 0.625237 6_4:-31623687 Msa0985290:five_prime_UTR
GGTGAAGAGTAGATATAATG+TGG 0.631465 6_4:-31623652 Msa0985290:exon
GGAGGTGGTGAATAGACAGG+AGG 0.632836 6_4:-31623577 Msa0985290:three_prime_UTR
GAAGAGTAGATATAATGTGG+TGG 0.646587 6_4:-31623649 Msa0985290:exon
TCCTTACCACTACTAAATGG+TGG 0.648183 6_4:+31623681 None:intergenic
GACTTGTACTTGTAAACTGG+TGG 0.657109 6_4:-31623292 Msa0985290:three_prime_UTR
CGTAGGAAGAAGTATACCGT+GGG 0.663811 6_4:-31623717 None:intergenic
GACTTGTACTTGTAAACTGG+GGG 0.672160 6_4:-31623547 Msa0985290:three_prime_UTR
ACGTAGGAAGAAGTATACCG+TGG 0.705296 6_4:-31623718 None:intergenic
TGGTTTACGATAGTATCCCA+CGG 0.713514 6_4:+31623701 None:intergenic
TGACTTGTACTTGTAAACTG+GGG 0.718901 6_4:-31623548 Msa0985290:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GGTGAAGAGTAGATATAATG+TGG - chr6_4:31623306-31623325 Msa0985290:three_prime_UTR 35.0%
GAAGAGTAGATATAATGTGG+TGG - chr6_4:31623309-31623328 Msa0985290:three_prime_UTR 35.0%
GGAGACTTATACTTGTAAAC+TGG - chr6_4:31623357-31623376 Msa0985290:three_prime_UTR 35.0%
GACTTATACTTGTAAACTGG+TGG - chr6_4:31623360-31623379 Msa0985290:three_prime_UTR 35.0%
TTATACTTGTAAACTGGTGG+AGG - chr6_4:31623363-31623382 Msa0985290:three_prime_UTR 35.0%
! GTGACTTGTACTTGTAAACT+GGG - chr6_4:31623409-31623428 Msa0985290:three_prime_UTR 35.0%
! TGACTTGTACTTGTAAACTG+GGG - chr6_4:31623410-31623429 Msa0985290:three_prime_UTR 35.0%
! GTGACTTGTACTTGTAAACT+GGG - chr6_4:31623409-31623428 Msa0985290:three_prime_UTR 35.0%
! TGACTTGTACTTGTAAACTG+GGG - chr6_4:31623410-31623429 Msa0985290:three_prime_UTR 35.0%
GGAGACTTGTATTTGTAAAG+TGG - chr6_4:31623510-31623529 Msa0985290:intron 35.0%
GACTTGTATTTGTAAAGTGG+TGG - chr6_4:31623513-31623532 Msa0985290:intron 35.0%
TTGTATTTGTAAAGTGGTGG+AGG - chr6_4:31623516-31623535 Msa0985290:intron 35.0%
TGGTTTACGATAGTATCCCA+CGG + chr6_4:31623260-31623279 None:intergenic 40.0%
CGTAAACCACCATTTAGTAG+TGG - chr6_4:31623271-31623290 Msa0985290:three_prime_UTR 40.0%
ACCACCATTTAGTAGTGGTA+AGG - chr6_4:31623276-31623295 Msa0985290:three_prime_UTR 40.0%
TCCTTACCACTACTAAATGG+TGG + chr6_4:31623280-31623299 None:intergenic 40.0%
ACCATTTAGTAGTGGTAAGG+AGG - chr6_4:31623279-31623298 Msa0985290:three_prime_UTR 40.0%
TCCTCCTTACCACTACTAAA+TGG + chr6_4:31623283-31623302 None:intergenic 40.0%
ATTTAGTAGTGGTAAGGAGG+AGG - chr6_4:31623282-31623301 Msa0985290:three_prime_UTR 40.0%
GAGTAGATATAATGTGGTGG+AGG - chr6_4:31623312-31623331 Msa0985290:three_prime_UTR 40.0%
! TAGATATAATGTGGTGGAGG+TGG - chr6_4:31623315-31623334 Msa0985290:three_prime_UTR 40.0%
! GGTGACTTGTACTTGTAAAC+TGG - chr6_4:31623408-31623427 Msa0985290:three_prime_UTR 40.0%
GACTTGTACTTGTAAACTGG+GGG - chr6_4:31623411-31623430 Msa0985290:three_prime_UTR 40.0%
! GGTGACTTGTACTTGTAAAC+TGG - chr6_4:31623408-31623427 Msa0985290:three_prime_UTR 40.0%
GACTTGTACTTGTAAACTGG+GGG - chr6_4:31623411-31623430 Msa0985290:three_prime_UTR 40.0%
! TATTTGTAAAGTGGTGGAGG+TGG - chr6_4:31623519-31623538 Msa0985290:intron 40.0%
GACTTGTACTTGTAAACTGG+TGG - chr6_4:31623411-31623430 Msa0985290:three_prime_UTR 40.0%
TTGTACTTGTAAACTGGTGG+TGG - chr6_4:31623567-31623586 Msa0985290:three_prime_UTR 40.0%
GACTTGTACTTGTAAACTGG+TGG - chr6_4:31623411-31623430 Msa0985290:three_prime_UTR 40.0%
TTGTACTTGTAAACTGGTGG+AGG - chr6_4:31623567-31623586 Msa0985290:three_prime_UTR 40.0%
GACTTGTACTTGTAAACTGG+TGG - chr6_4:31623411-31623430 Msa0985290:three_prime_UTR 40.0%
TTGTACTTGTAAACTGGTGG+TGG - chr6_4:31623567-31623586 Msa0985290:three_prime_UTR 40.0%
TACTTGTAAACTGGTGGAGG+TGG - chr6_4:31623366-31623385 Msa0985290:three_prime_UTR 45.0%
TTGTACTTGTAAACTGGGGG+AGG - chr6_4:31623414-31623433 Msa0985290:three_prime_UTR 45.0%
TTGTACTTGTAAACTGGGGG+AGG - chr6_4:31623414-31623433 Msa0985290:three_prime_UTR 45.0%
GGGGACTTGTACTTGTAAAC+TGG - chr6_4:31623561-31623580 Msa0985290:three_prime_UTR 45.0%
!! TACTTGTAAACTGGTGGTGG+TGG - chr6_4:31623570-31623589 Msa0985290:three_prime_UTR 45.0%
GGGGACTTGTACTTGTAAAC+TGG - chr6_4:31623561-31623580 Msa0985290:three_prime_UTR 45.0%
TACTTGTAAACTGGTGGAGG+TGG - chr6_4:31623366-31623385 Msa0985290:three_prime_UTR 45.0%
GGGGACTTGTACTTGTAAAC+TGG - chr6_4:31623561-31623580 Msa0985290:three_prime_UTR 45.0%
!! TACTTGTAAACTGGTGGTGG+TGG - chr6_4:31623570-31623589 Msa0985290:three_prime_UTR 45.0%
TAGTAGTGGTAAGGAGGAGG+TGG - chr6_4:31623285-31623304 Msa0985290:three_prime_UTR 50.0%
TACTTGTAAACTGGGGGAGG+TGG - chr6_4:31623417-31623436 Msa0985290:three_prime_UTR 50.0%
TACTTGTAAACTGGGGGAGG+TGG - chr6_4:31623417-31623436 Msa0985290:three_prime_UTR 50.0%
GGTGGAGGTGGTGAATAGAC+AGG - chr6_4:31623378-31623397 Msa0985290:three_prime_UTR 55.0%
GGAGGTGGTGAATAGACAGG+AGG - chr6_4:31623381-31623400 Msa0985290:three_prime_UTR 55.0%
GGTGGTGAATAGACAGGAGG+AGG - chr6_4:31623384-31623403 Msa0985290:three_prime_UTR 55.0%
GGTGAATAGACAGGAGGAGG+TGG - chr6_4:31623387-31623406 Msa0985290:three_prime_UTR 55.0%
TGGTGGAGGTGGTGAGTAGA+CGG - chr6_4:31623530-31623549 Msa0985290:intron 55.0%
!! TGGTGGTGGTGGTGAGTAGA+CGG - chr6_4:31623581-31623600 Msa0985290:three_prime_UTR 55.0%
TGGTGGAGGTGGTGAGTAGA+CGG - chr6_4:31623530-31623549 Msa0985290:intron 55.0%
!! TGGTGGTGGTGGTGAGTAGA+CGG - chr6_4:31623581-31623600 Msa0985290:three_prime_UTR 55.0%
GGTGGAGGTGGTGAGTAAGC+AGG - chr6_4:31623327-31623346 Msa0985290:three_prime_UTR 60.0%
GGAGGTGGTGAGTAAGCAGG+AGG - chr6_4:31623330-31623349 Msa0985290:three_prime_UTR 60.0%
GGTGGTGAGTAAGCAGGAGG+AGG - chr6_4:31623333-31623352 Msa0985290:three_prime_UTR 60.0%
GGTGAGTAAGCAGGAGGAGG+AGG - chr6_4:31623336-31623355 Msa0985290:three_prime_UTR 60.0%
TGGGGGAGGTGGTGAGTAGA+CGG - chr6_4:31623428-31623447 Msa0985290:three_prime_UTR 60.0%
GGAGGTGGTGAGTAGACAGG+TGG - chr6_4:31623483-31623502 Msa0985290:intron 60.0%
GGTGGTGAGTAGACAGGTGG+AGG - chr6_4:31623486-31623505 Msa0985290:intron 60.0%
GGTGAGTAGACAGGTGGAGG+AGG - chr6_4:31623489-31623508 Msa0985290:intron 60.0%
GGTGGAGGTGGTGAGTAGAC+GGG - chr6_4:31623531-31623550 Msa0985290:intron 60.0%
GTGAGTAGACGGGTGGAGGT+GGG - chr6_4:31623541-31623560 Msa0985290:intron 60.0%
TGAGTAGACGGGTGGAGGTG+GGG - chr6_4:31623542-31623561 Msa0985290:three_prime_UTR 60.0%
!! GGTGGTGGTGGTGAGTAGAC+GGG - chr6_4:31623582-31623601 Msa0985290:three_prime_UTR 60.0%
GTGAGTAGACGGGTGGAGGT+GGG - chr6_4:31623541-31623560 Msa0985290:intron 60.0%
TGAGTAGACGGGTGGAGGTG+GGG - chr6_4:31623542-31623561 Msa0985290:three_prime_UTR 60.0%
GGTGGAGGTGGTGAGTAGAC+GGG - chr6_4:31623531-31623550 Msa0985290:intron 60.0%
GTGAGTAGACGGGTGGAGGA+GGG - chr6_4:31623643-31623662 Msa0985290:exon 60.0%
TGAGTAGACGGGTGGAGGAG+GGG - chr6_4:31623644-31623663 Msa0985290:exon 60.0%
!! GGTGGTGGTGGTGAGTAGAC+GGG - chr6_4:31623582-31623601 Msa0985290:three_prime_UTR 60.0%
GTGAGTAGACGGGTGGAGGT+GGG - chr6_4:31623541-31623560 Msa0985290:intron 60.0%
TGAGTAGACGGGTGGAGGTG+GGG - chr6_4:31623542-31623561 Msa0985290:three_prime_UTR 60.0%
GGGGGAGGTGGTGAGTAGAC+GGG - chr6_4:31623429-31623448 Msa0985290:three_prime_UTR 65.0%
GGAGGTGGTGAGTAGACGGG+AGG - chr6_4:31623432-31623451 Msa0985290:three_prime_UTR 65.0%
GGTGGTGAGTAGACGGGAGG+AGG - chr6_4:31623435-31623454 Msa0985290:three_prime_UTR 65.0%
GGTGAGTAGACGGGAGGAGG+TGG - chr6_4:31623438-31623457 Msa0985290:three_prime_UTR 65.0%
GGGGGAGGTGGTGAGTAGAC+AGG - chr6_4:31623429-31623448 Msa0985290:three_prime_UTR 65.0%
GGAGGTGGTGAGTAGACGGG+TGG - chr6_4:31623432-31623451 Msa0985290:three_prime_UTR 65.0%
GGTGGTGAGTAGACGGGTGG+AGG - chr6_4:31623537-31623556 Msa0985290:intron 65.0%
GGTGAGTAGACGGGTGGAGG+TGG - chr6_4:31623540-31623559 Msa0985290:intron 65.0%
! GGTGGTGGTGAGTAGACGGG+TGG - chr6_4:31623585-31623604 Msa0985290:three_prime_UTR 65.0%
GGTGGTGAGTAGACGGGTGG+AGG - chr6_4:31623537-31623556 Msa0985290:intron 65.0%
GGTGAGTAGACGGGTGGAGG+TGG - chr6_4:31623540-31623559 Msa0985290:intron 65.0%
GGAGGTGGTGAGTAGACGGG+TGG - chr6_4:31623432-31623451 Msa0985290:three_prime_UTR 65.0%
GGTGGTGAGTAGACGGGTGG+AGG - chr6_4:31623537-31623556 Msa0985290:intron 65.0%
GGTGAGTAGACGGGTGGAGG+AGG - chr6_4:31623540-31623559 Msa0985290:intron 65.0%
! GGTGGTGGTGAGTAGACGGG+TGG - chr6_4:31623585-31623604 Msa0985290:three_prime_UTR 65.0%
GGTGGTGAGTAGACGGGTGG+AGG - chr6_4:31623537-31623556 Msa0985290:intron 65.0%
GGTGAGTAGACGGGTGGAGG+TGG - chr6_4:31623540-31623559 Msa0985290:intron 65.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 31623246 31623734 31623246 ID=Msa0985290;Name=Msa0985290
chr6_4 mRNA 31623246 31623734 31623246 ID=Msa0985290-mRNA-1;Parent=Msa0985290;Name=Msa0985290-mRNA-1;_AED=0.28;_eAED=0.52;_QI=77|0|0|1|0|0|2|331|5
chr6_4 exon 31623542 31623734 31623542 ID=Msa0985290-mRNA-1:exon:8454;Parent=Msa0985290-mRNA-1
chr6_4 exon 31623246 31623478 31623246 ID=Msa0985290-mRNA-1:exon:8453;Parent=Msa0985290-mRNA-1
chr6_4 five_prime_UTR 31623658 31623734 31623658 ID=Msa0985290-mRNA-1:five_prime_utr;Parent=Msa0985290-mRNA-1
chr6_4 CDS 31623640 31623657 31623640 ID=Msa0985290-mRNA-1:cds;Parent=Msa0985290-mRNA-1
chr6_4 three_prime_UTR 31623542 31623639 31623542 ID=Msa0985290-mRNA-1:three_prime_utr;Parent=Msa0985290-mRNA-1
chr6_4 three_prime_UTR 31623246 31623478 31623246 ID=Msa0985290-mRNA-1:three_prime_utr;Parent=Msa0985290-mRNA-1
Gene Sequence

>Msa0985290

ATGTGGTGGAGGTGGTGA

Protein sequence

>Msa0985290

MWWRW*